Research Article |
Corresponding author: Ning Jiang ( n.jiang@caf.ac.cn ) Corresponding author: Yong Li ( lylx@caf.ac.cn ) Academic editor: Nattawut Boonyuen
© 2022 Ning Jiang, Hermann Voglmayr, Chun-Yan Ma, Han Xue, Chun-Gen Piao, Yong Li.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Jiang N, Voglmayr H, Ma C-Y, Xue H, Piao C-G, Li Y (2022) A new Arthrinium-like genus of Amphisphaeriales in China. MycoKeys 92: 27-43. https://doi.org/10.3897/mycokeys.92.86521
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Species of Arthrinium s. l. are usually known as endophytes, pathogens or saprobes occurring on various hosts and substrates and are characterised by globose to subglobose, sometimes irregular, dark brown and smooth-walled or finely verruculose conidia, always with a truncate basal scar. Currently, Arthrinium s. l. contains two phylogenetically distinct clades, namely, Apiospora and Arthrinium s. s. However, Arthrinium trachycarpi and Ar. urticae have still not been properly classified. With new isolates from diseased leaves of Lithocarpus glaber collected in China, we propose the new Arthrinium-like genus Neoarthrinium in Amphisphaeriales. Based on the morphology and phylogeny of multiple loci, the new genus is established with the type species, N. lithocarpicola and three new combinations, N. moseri (syn. Wardomyces moseri), N. trachycarpi (syn. Ar. trachycarpi) and N. urticae (syn. Ar. urticae) are added to this genus.
Apiospora, Arthrinium, Neoarthrinium, phylogeny, taxonomy
Apiosporaceae, including Arthrinium-like taxa, was proposed to accommodate genera with apiosporous hyaline ascospores and a basauxic, Arthrinium-like conidiogenesis (
Until the study of
Arthrinium-like species are globally distributed, inhabiting various substrates, mainly associated with plant tissues as endophytes, pathogens and saprobes (
In the present study, new Arthrinium-like isolates were collected and morphologically examined and their phylogenetic affiliation was determined by analyses of a combined matrix of ITS, LSU, tef1 and tub2 sequences. The aim of this study was to determine the phylogenetic placement of Ar. trachycarpi, Ar. urticae and our new isolates within Amphisphaeriales, which resulted in the identification of a new phylogenetic lineage with isolates belonging to neither Arthrinium nor Apiospora. As a result, a new genus is established for these isolates.
Diseased leaves of Lithocarpus glaber were observed and collected in Guangdong Province of China (39 m elevation; 23°8'52"N, 113°27'18"E), packed in paper bags and transferred to the laboratory for pure culture isolation. The samples were first surface-sterilised for 1 min in 75% ethanol, 3 min in 1.25% sodium hypochlorite and 1 min in 75% ethanol, rinsed for 2 min in distilled water and blotted on dry sterile filter paper. Then, the diseased areas of the leaves were cut into 0.5 × 0.5 cm pieces using an aseptic razor blade, transferred on to the surface of potato dextrose agar plates (PDA; 200 g potatoes, 20 g dextrose, 20 g agar per litre) and incubated at 25 °C to obtain pure cultures. The cultures were deposited in the China Forestry Culture Collection Center (CFCC; http://cfcc.caf.ac.cn/) and the specimen was deposited in the Herbarium of the Chinese Academy of Forestry (
The morphology of the isolates was studied, based on sporulating axenic cultures grown on PDA in the dark at 25 °C. The conidiomata were observed and photographed under a dissecting microscope (M205 C, Leica, Wetzlar, Germany). The conidiogenous cells and conidia were immersed in tap water and then the microscopic photographs were captured with an Axio Imager 2 microscope (Zeiss, Oberkochen, Germany), equipped with an Axiocam 506 colour camera using differential interference contrast (DIC) illumination. For measurements, 50 conidiogenous cells and conidia were randomly selected. Culture characteristics were recorded from PDA after 10 d of incubation at 25 °C in the dark.
Genomic DNA was extracted from colonies grown on cellophane-covered PDA using a cetyltrimethylammonium bromide (CTAB) method (
The quality of the chromatograms obtained was checked and the nucleotide sequences were assembled using SeqMan v.7.1.0, the DNASTAR lasergene core suite software (DNASTAR Inc, Madison, WI, USA). Reference sequences were retrieved from the National Center for Biotechnology Information (NCBI; https://www.ncbi.nlm.nih.gov), based on related publications (
The phylogenetic analyses of the combined loci were performed using Maximum Likelihood (ML) and Bayesian Inference (BI) methods. The ML was implemented on the CIPRES Science Gateway portal (https://www.phylo.org) using RAxML-HPC BlackBox 8.2.10 (
Isolates and GenBank accession numbers used in the phylogenetic analyses.
Species | Strain | Host | Origin | GenBank accession numbers | |||
---|---|---|---|---|---|---|---|
ITS | LSU | tub2 | tef1 | ||||
Allelochaeta acuta | CPC 16629 | Eucalyptus dives | Australia | MH554086 | MH554297 | MH554758 | MH554519 |
Allelochaeta neoacuta | CBS 115131 | Eucalyptus smithii | South Africa | JN871200 | JN871209 | MH704627 | MH704602 |
Amphisphaeria micheliae | MFLUCC 20-0121 | Michelia alba | China | MT756626 | MT756620 | MT774371 | NA |
Apiospora acutiapica | KUMCC 20-0209 | Bambusa bambos | China | MT946342 | MT946338 | MT947365 | MT947359 |
Apiospora acutiapica | KUMCC 20-0210 | Bambusa bambos | China | MT946343 | MT946339 | MT947366 | MT947360 |
Apiospora arundinis | CBS 114316 | Hordeum vulgare | Iran | KF144884 | KF144928 | KF144974 | KF145016 |
Apiospora aurea | CBS 244.83 | Air | Spain | AB220251 | KF144935 | KF144981 | KF145023 |
Apiospora balearica | CBS 145129 | Poaceae | Spain | MK014869 | MK014836 | MK017975 | NA |
Apiospora biserialis | CGMCC 3.20135 | Bamboo | China | MW481708 | MW478885 | MW522955 | MW522938 |
Apiospora camelliae-sinensis | LC8181 | Brassica campestris | China | KY494761 | KY494837 | KY705229 | NA |
Apiospora camelliae-sinensis | CGMCC 3.18333 | Camellia sinensis | China | KY494704 | KY494780 | KY705173 | KY705103 |
Apiospora cyclobalanopsidis | CGMCC 3.20136 | Cyclobalanopsis glauca | China | MW481713 | MW478892 | MW522962 | MW522945 |
Apiospora descalsii | CBS 145130 | Ampelodesmos mauritanicus | Spain | MK014870 | MK014837 | MK017976 | NA |
Apiospora dichotomanthi | LC8175 | Dichotomanthes tristaniiaecarpa | China | KY494755 | KY494831 | KY705223 | KY705151 |
Apiospora dichotomanthi | CGMCC 3.18332 | Dichotomanthes tristaniiaecarpa | China | KY494697 | KY494773 | KY705167 | KY705096 |
Apiospora esporlensis | CBS 145136 | Phyllostachys aurea | Spain | MK014878 | MK014845 | MK017983 | NA |
Apiospora gelatinosa | GZAAS 20-0107 | Bamboo | China | MW481707 | MW478889 | MW522959 | MW522942 |
Apiospora guizhouensis | LC5318 | Air | China | KY494708 | KY494784 | KY705177 | KY705107 |
Apiospora guizhouensis | CGMCC 3.18334 | Air | China | KY494709 | KY494785 | KY705178 | KY705108 |
Apiospora hydei | CBS 114990 | Bamboo | China | KF144890 | KF144936 | KF144982 | KF145024 |
Apiospora iberica | CBS 145137 | Arundo donax | Portugal | MK014879 | MK014846 | MK017984 | NA |
Apiospora intestini | CBS 135835 | Gut of a grasshopper | India | KR011352 | MH877577 | KR011350 | NA |
Apiospora italica | CBS 145138 | Arundo donax | Italy | MK014880 | MK014847 | MK017985 | NA |
Apiospora jiangxiensis | CGMCC 3.18381 | Maesa sp. | China | KY494693 | KY494769 | KY705163 | KY705092 |
Apiospora kogelbergensis | CBS 113332 | Cannomois virgata | South Africa | KF144891 | KF144937 | KF144983 | KF145025 |
Apiospora kogelbergensis | CBS 113333 | Restionaceae | South Africa | KF144892 | KF144938 | KF144984 | KF145026 |
Apiospora malaysiana | CBS 102053 | Macaranga hullettii | Malaysia | KF144896 | KF144942 | KF144988 | KF145030 |
Apiospora marii | CBS 497.90 | Air | Spain | AB220252 | KF144947 | KF144993 | KF145035 |
Apiospora neobambusae | CGMCC 3.18335 | Bamboo | China | KY494718 | KY494794 | KY705186 | KY806204 |
Apiospora neobambusae | LC7107 | Bamboo | China | KY494719 | KY494795 | KY705187 | KY705117 |
Apiospora obovata | CGMCC 3.18331 | Lithocarpus sp. | China | KY494696 | KY494772 | KY705166 | KY705095 |
Apiospora obovata | LC8177 | Lithocarpus sp. | China | KY494757 | KY494833 | KY705225 | KY705153 |
Apiospora ovata | CBS 115042 | Arundinaria hindsii | China | KF144903 | KF144950 | KF144995 | KF145037 |
Apiospora phragmitis | CBS 135458 | Phragmites australis | Italy | KF144909 | KF144956 | KF145001 | KF145043 |
Apiospora phyllostachydis | MFLUCC 18-1101 | Phyllostachys heteroclada | China | MK351842 | MH368077 | MK291949 | MK340918 |
Apiospora pseudoparenchymatica | CGMCC 3.18336 | Bamboo | China | KY494743 | KY494819 | KY705211 | KY705139 |
Apiospora pseudospegazzinii | CBS 102052 | Macaranga hullettii | Malaysia | KF144911 | KF144958 | KF145002 | KF145045 |
Apiospora pterosperma | CBS 134000 | Machaerina sinclairii | Australia | KF144913 | KF144960 | KF145004 | KF145046 |
Apiospora saccharicola | CBS 191.73 | Air | Netherlands | KF144920 | KF144966 | KF145009 | KF145051 |
Apiospora septata | CGMCC 3.20134 | Bamboo | China | MW481711 | MW478890 | MW522960 | MW522943 |
Apiospora serenensis | IMI 326869 | NA | Spain | AB220250 | AB220344 | AB220297 | NA |
Apiospora subrosea | LC7291 | Bamboo | China | KY494751 | KY494827 | KY705219 | KY705147 |
Apiospora subrosea | CGMCC 3.18337 | Bamboo | China | KY494752 | KY494828 | KY705220 | KY705148 |
Apiospora xenocordella | CBS 595.66 | Soil | Austria | KF144926 | KF144971 | KF145013 | KF145055 |
Arthrinium caricicola | CBS 145127 | Carex ericetorum | Germany | MK014871 | MK014838 | MK017977 | NA |
Arthrinium crenatum | CBS 146353 | Grass | France | MW208931 | MW208861 | MW221923 | MW221917 |
Arthrinium curvatum | CBS 145131 | Carex sp. | Germany | MK014872 | MK014839 | MK017978 | NA |
Arthrinium japonicum | IFO 30500 | Carex despalata | Japan | AB220262 | AB220356 | AB220309 | NA |
Arthrinium japonicum | IFO 31098 | Carex despalata | Japan | AB220264 | AB220358 | AB220311 | NA |
Arthrinium luzulae | AP7619-3 | Luzula sylvatica | Spain | MW208937 | MW208863 | MW221925 | MW221919 |
Arthrinium morthieri | GZU 345043 | Carex digitata | Austria | MW208938 | MW208864 | MW221926 | MW221920 |
Arthrinium puccinioides | CBS 549.86 | Lepidosperma gladiatum | Germany | AB220253 | AB220347 | AB220300 | NA |
Arthrinium sphaerospermum | CBS 146355 | Poaceae | Norway | MW208943 | MW208865 | NA | NA |
Arthrinium sporophleum | CBS 145154 | Juncus sp. | Spain | MK014898 | MK014865 | MK018001 | NA |
Bartalinia bella | CBS 125525 | Maytenus abbottii | South Africa | GU291796 | MH554214 | MH554663 | MH554421 |
Bartalinia pini | CBS 143891 | Pinus patula | Uganda | MH554125 | MH554330 | MH554797 | MH554559 |
Beltrania pseudorhombica | CBS 138003 | Pinus tabulaeformis | China | MH554124 | KJ869215 | NA | MH554558 |
Beltrania rhombica | CBS 123.58 | Sand near mangrove swamp | Mozambique | MH553990 | MH554209 | MH704631 | MH704606 |
Beltraniopsis neolitseae | CPC 22168 | Neolitsea australiensis | Australia | KJ869126 | KJ869183 | NA | NA |
Broomella vitalbae | HPC 1154 | NA | NA | MH554173 | MH554367 | MH554846 | MH554608 |
Castanediella cagnizarii | CBS 542.96 | Leaf litter | Cuba | MH862597 | MH874222 | NA | NA |
Ciliochorella phanericola | MFLUCC 12-0310 | Dead leaves | Thailand | KF827444 | KF827445 | KF827478 | KF827477 |
Clypeophysalospora latitans | CBS 141463 | Eucalyptus sp. | Portugal | NR_153929 | NG_058958 | NA | NA |
Clypeosphaeria mamillana | CBS 140735 | Cornus alba | France | KT949897 | MH554225 | MH704637 | MH704610 |
Cylindrium elongatum | CBS 115974 | Fagus sp. | The Netherlands | KM231853 | KM231733 | KM232123 | KM231989 |
Diploceras hypericinum | CBS 109058 | Hypericum sp. | New Zealand | MH553955 | MH554178 | MH554614 | MH554373 |
Disaeta arbuti | CBS 143903 | Acacia pycnantha | Australia | MH554148 | MH554346 | MH554821 | MH554583 |
Discosia artocreas | CBS 124848 | Fagus sylvatica | Germany | MH553994 | MH554213 | MH554662 | MH554420 |
Discosia brasiliensis | MFLUCC 12-0429 | Dead leaf | Thailand | KF827432 | KF827436 | KF827469 | KF827465 |
Distononappendiculata banksiae | CBS 131308 | Banksia marginata | Australia | JQ044422 | JQ044442 | MH554670 | MH554428 |
Distononappendiculata casuarinae | CBS 143884 | Casuarina sp. | Australia | MH554093 | MH554303 | MH554766 | MH554527 |
Diversimediispora humicola | CBS 302.86 | Soil | USA | MH554028 | MH554247 | MH554705 | MH554463 |
Heterotruncatella acacigena | CBS 143880 | Acacia pedina | Australia | MH554084 | MH554295 | MH554756 | MH554517 |
Heterotruncatella aspera | CBS 144140 | Acacia glaucoptera | Australia | MH554156 | MH554352 | MH554829 | MH554591 |
Hyalotiella spartii | MFLUCC 13-0397 | Spartium junceum | Italy | KP757756 | KP757752 | NA | NA |
Hyalotiella transvalensis | CBS 303.65 | Leaf litter and topsoil of Acacia karroo community | South Africa | MH554029 | MH554248 | MH554706 | MH554464 |
Hymenopleella austroafricana | CBS 143886 | Gleditsia triacanthos | South Africa | MH554115 | MH554320 | MH554788 | MH554549 |
Hymenopleella hippophaëicola | CBS 113687 | Hippophaë rhamnoides | Sweden | MH553969 | MH554188 | MH554628 | MH554387 |
Immersidiscosia eucalypti | NBRC 104195 | Quercus myrsinifolia | Japan | AB594790 | AB593722 | NA | NA |
Lepteutypa fuckelii | CBS 140409 | Tilia cordata | Belgium | NR_154123 | KT949902 | MH554677 | MH554435 |
Lepteutypa sambuci | CBS 131707 | Sambucus nigra | UK | NR_154124 | MH554219 | MH704632 | MH704612 |
Monochaetia monochaeta | CBS 115004 | Quercus robur | Netherlands | AY853243 | MH554198 | MH554639 | MH554398 |
Monochaetia quercus | CBS 144034 | Quercus eduardi | Mexico | MH554171 | MH554365 | MH554844 | MH554606 |
Morinia acaciae | CBS 137994 | Acacia melanoxylon | France | MH554002 | MH554221 | MH554673 | MH554431 |
Morinia crini | CBS 143888 | Crinum bulbispermum | South Africa | MH554118 | MH554323 | MH554791 | MH554552 |
Neoarthrinium lithocarpicola | CFCC 54456 | Lithocarpus glaber | China | ON427580 | ON427582 | ON456914 | NA |
Neoarthrinium lithocarpicola | CFCC 55883 | Lithocarpus glaber | China | ON427581 | ON427583 | ON456915 | NA |
Noarthrinium moseri | CBS 164.80 | Dead petiole | Colombia | LN850995 | LN851049 | LN851154 | NA |
Neoarthrinium trachycarpi | CFCC 53038 | Trachycarpus fortunei | China | MK301098 | NA | MK303394 | MK303396 |
Neoarthrinium trachycarpi | CFCC 53039 | Trachycarpus fortunei | China | MK301099 | NA | MK303395 | MK303397 |
Neoarthrinium urticae | IMI 326344 | Leaf litter | India | AB220245 | AB220339 | NA | NA |
Neopestalotiopsis cubana | CBS 600.96 | Leaf litter | Cuba | KM199347 | KM116253 | KM199438 | KM199521 |
Neophysalospora eucalypti | CBS 138864 | Corymbia henryi | Mozambique | KP004462 | MH878627 | NA | NA |
Nigrospora aurantiaca | CGMCC 3.18130 | Nelumbo sp. | China | KX986064 | KX986098 | KY019465 | KY019295 |
Nigrospora camelliae-sinensis | CGMCC 3.18125 | Camellia sinensis | China | KX985986 | KX986103 | KY019460 | KY019293 |
Nigrospora chinensis | CGMCC 3.18127 | Machilus breviflora | China | KX986023 | KX986107 | KY019462 | KY019422 |
Nigrospora gorlenkoana | CBS 480.73 | Vitis vinifera | Kazakhstan | KX986048 | KX986109 | KY019456 | KY019420 |
Nigrospora guilinensis | CGMCC 3.18124 | Camellia sinensis | China | KX985983 | KX986113 | KY019459 | KY019292 |
Nigrospora hainanensis | CGMCC 3.18129 | Musa paradisiaca | China | KX986091 | KX986112 | KY019464 | KY019415 |
Nigrospora lacticolonia | CGMCC 3.18123 | Camellia sinensis | China | KX985978 | KX986105 | KY019458 | KY019291 |
Nigrospora musae | CBS 319.34 | Musa sp. | Australia | MH855545 | KX986110 | KY019455 | KY019419 |
Nigrospora oryzae | LC2693 | Neolitsea sp. | China | KX985944 | KX986101 | KY019471 | KY019299 |
Nigrospora osmanthi | CGMCC 3.18126 | Osmanthus sp. | China | KX986010 | KX986106 | KY019461 | KY019421 |
Nigrospora pyriformis | CGMCC 3.18122 | Citrus sinensis | China | KX985940 | KX986100 | KY019457 | KY019290 |
Nigrospora rubi | LC2698 | Rubus sp. | China | KX985948 | KX986102 | KY019475 | KY019302 |
Nigrospora sphaerica | LC7298 | Nelumbo sp. | China | KX985937 | KX986097 | KY019606 | KY019401 |
Nigrospora vesicularis | CGMCC 3.18128 | Musa paradisiaca | China | KX986088 | KX986099 | KY019463 | KY019294 |
Nonappendiculata quercina | CBS 116061 | Quercus suber | Italy | MH553982 | MH554199 | MH554641 | MH554400 |
Parabartalinia lateralis | CBS 399.71 | Acacia karroo | South Africa | MH554043 | MH554256 | MH554719 | MH554478 |
Parapleurotheciopsis inaequiseptata | MUCL 41089 | Rotten leaf | Brazil | EU040235 | EU040235 | NA | NA |
Parapleurotheciopsis caespitosa | CBS 519.93 | Syzygium cordatum | South Africa | MH862437 | NG_066263 | NA | NA |
Pestalotiopsis adusta | CBS 263.33 | Rhododendron ponticum | Netherlands | KM199316 | KM116198 | KM199414 | KM199489 |
Pestalotiopsis australasiae | CBS 114126 | Knightia sp. | New Zealand | KM199297 | KM116218 | KM199409 | KM199499 |
Phlogicylindrium eucalypti | CBS 120080 | Eucalyptus globulus | Australia | NR_132813 | DQ923534 | MH704633 | MH704607 |
Phlogicylindrium eucalyptorum | CBS 120221 | Eucalyptus globus | Australia | EU040223 | MH554204 | MH704635 | MH704608 |
Pseudopestalotiopsis ampullacea | LC6618 | Camellia sinensis | China | KX895025 | KX895039 | KX895358 | KX895244 |
Pseudopestalotiopsis camelliae-sinensis | LC3009 | Camellia sinensis | China | KX894935 | KX895050 | KX895267 | KX895152 |
Pseudosarcostroma osyridicola | CBS 103.76 | Osyris alba | France | MH553954 | MH554177 | MH554613 | MH554372 |
Pseudosporidesmium knawiae | CBS 123529 | NA | NA | MH863299 | MH874823 | NA | NA |
Robillarda africana | CBS 122.75 | NA | South Africa | KR873253 | KR873281 | MH554656 | MH554414 |
Robillarda terrae | CBS 587.71 | Soil | India | KJ710484 | KJ710459 | MH554734 | MH554493 |
Sarcostroma africanum | CBS 143879 | Pelargonium cucullatum | South Africa | MH554078 | MH554289 | MH554752 | MH554513 |
Sarcostroma australiense | CBS 144160 | Daviesia latifolia | Australia | MH554138 | MH554340 | MH554811 | MH554573 |
Seimatosporium germanicum | CBS 437.87 | NA | Germany | MH554047 | MH554259 | MH554723 | MH554482 |
Seimatosporium luteosporum | CBS 142599 | Vitis vinifera | USA | KY706284 | KY706309 | KY706259 | KY706334 |
Seiridium cancrinum | CBS 226.55 | Cupressus macrocarpa | Kenya | LT853089 | MH554241 | LT853236 | LT853186 |
Seiridium cupressi | CBS 224.55 | Cupressus macrocarpa | Kenya | LT853083 | MH554240 | LT853230 | LT853180 |
Sporocadus biseptatus | CBS 110324 | NA | NA | MH553956 | MH554179 | MH554615 | MH554374 |
Sporocadus cornicola | CBS 143889 | Cornus sanguinea | Germany | MH554121 | MH554326 | MH554794 | MH554555 |
Sporocadus trimorphus | CBS 114203 | Rosa canina | Sweden | MH553977 | MH554196 | MH554636 | MH554395 |
Strickeria kochii | CBS 140411 | Robinia pseudoacacia | Austria | NR_154423 | KT949918 | MH554679 | MH554437 |
Subramaniomyces fusisaprophyticus | CBS 418.95 | Leaf litter | Cuba | EU040241 | EU040241 | NA | NA |
Synnemapestaloides juniperi | CBS 477.77 | Juniperus phoenicea | France | MH554053 | MH554266 | MH554729 | MH554488 |
Truncatella angustata | CBS 113.11 | Picea abies | Germany | MH553966 | MH554185 | MH554625 | MH554384 |
Xenoseimatosporium quercinum | CBS 129171 | Rhododendron sp. | Latvia | MH553997 | MH554216 | MH554666 | MH554424 |
Xyladictyochaeta lusitanica | CBS 143502 | Eucalyptus sp. | Australia | MH107926 | MH107972 | MH108053 | MH108033 |
The combined sequence dataset (ITS, LSU, tef1 and tub2) was analysed to infer the phylogenetic placement of our new isolates within Amphisphaeriales. The dataset consisted of 136 sequences, including two outgroup taxa, Clypeosphaeria mamillana (CBS 140735) and Pseudosporidesmium knawiae (CBS 123529). A total of 3526 characters, including gaps (793 for ITS, 859 for LSU, 762 for tef1 and 1112 for tub2), were included in the phylogenetic analysis. Of these characters, 1543 were constant, 284 were variable, but parsimony-uninformative and 1699 were parsimony-informative. The best ML tree (lnL = - 72640.48) revealed by RAxML is shown in Fig.
Phylogram of Amphisphaeriales resulting from a Maximum Likelihood analysis, based on a combined matrix of ITS, LSU, tef1 and tub2. Numbers above the branches indicate ML bootstraps (left, ML BS ≥ 50%) and Bayesian Posterior Probabilities (right, BPP ≥ 0.90). The tree is rooted with Clypeosphaeria mamillana (CBS 140735) and Pseudosporidesmium knawiae (CBS 123529). New species and combinations proposed in the present study are marked in blue.
Named after its morphological similarity to Arthrinium.
Neoarthrinium lithocarpicola Ning Jiang
Hyphae formed on PDA hyaline, branched, septate. Asexual morph: Conidiophores cylindrical, septate, verrucose, flexuous, sometimes reduced to conidiogenous cells. Conidiogenous cells erect, blastic, aggregated in clusters on hyphae, hyaline to pale brown, smooth, doliiform, subglobose to lageniform, branched. Conidia brown to dark brown, smooth to finely roughened, subglobose, ellipsoid to lenticular, with a longitudinal germ slit, occasionally elongated to ellipsoidal. Sexual morph: Undetermined.
Named for its host genus “Lithocarpus” and “-cola” = inhabiting.
Hyphae 1.5–4.5 μm diam., hyaline, branched, septate. Asexual morph: Conidiophores cylindrical, septate, verrucose, flexuous, sometimes reduced to conidiogenous cells. Conidiogenous cells erect, blastic, aggregated in clusters on hyphae, hyaline to pale brown, smooth, globose to subglobose, branched, (4–)5.5–8 × 2.5–3.5(–4) μm, mean ± SD = 6.6 ± 1.3 × 3.1 ± 0.4 μm, n = 50. Conidia brown to dark brown, smooth to finely roughened, subglobose to lenticular, with a longitudinal germ slit, occasionally elongated to ellipsoidal, (5–)6–8(–8.5) × (4.5–)5–5.5(–6) μm, mean ± SD = 7 ± 0.8 × 5.3 ± 0.5 μm, L/W = 1.1–1.8, n = 50. Sexual morph: Undetermined.
Colonies on PDA flat, spreading, with flocculent aerial mycelium forming concentric rings, edge entire, mouse grey to greyish-green, reaching 60 mm diam. after 10 d at 25 °C, forming abundant conidiomata.
China. Guangdong Province, Guangzhou City, on leaf spots of Lithocarpus glaber (Thunb.) Nakai, Shang Sun (holotype CAF800050 = JNH0046; ex-type living culture: CFCC 54456; other living culture: CFCC 55883).
Two isolates of Neoarthrinium lithocarpicola from Lithocarpus glaber (Thunb.) Nakai formed a well-supported monophyletic clade, distinct from N. moseri, N. trachycarpi and N. urticae (Fig.
Wardomyces moseri W. Gams, Beih. Sydowia 10: 67 (1995)
Based on a placement within Xylariales in phylogenetic analyses,
Arthrinium trachycarpi C.M. Tian & H. Yan [as ‘trachycarpum’], Phytotaxa 400(3): 208 (2019)
Arthrinium urticae M.B. Ellis, Mycol. Pap. 103: 16 (1965)
The possibility that Apiosporella urticae (Rehm) Höhn. is the sexual morph of Arthrinium urticae is raised by the fact that both share the same host (Urtica) and are classified as members of the Apiosporaceae (Index Fungorum, accessed 4 July 2022). This evidence would have far reaching nomenclatural consequences not only for species, but also for generic classification, as Apiosporella (
No sequence data are available for isolates from the type host Urtica dioica L. (Urticaceae). The single culture sequenced (IMI 326344) was isolated from unidentified leaf litter collected in India. Additional molecular studies on verified isolates from Urtica collected in Europe are necessary to reveal whether IMI 326344 represents true N. urticae. However, N. urticae appears to be very rare and we are unaware of any additional collections with the exception of the type.
Arthrinium and related genera are important fungal taxa whose concepts and classification have undergone many changes and additions (e.g.
Morphologically, Apiospora, Arthrinium and Neoarthrinium are similar in having basauxic conidiogenesis. Conidia of Apiospora and Neoarthrinium are generally more or less rounded in face view and lenticular in side view, while those of Arthrinium are variously shaped, viz. globose, angular, polygonal, curved, fusiform or navicular (
Regarding their hosts, there are some tendencies in host preferences, while Arthrinium species are predominantly found in Cyperaceae and Juncaceae (
Compared to species, generic delimitation is much more subjective. However, there is a broad agreement that genera, along with all taxonomic classification units at all ranks, should be monophyletic. As morphology is frequently insufficient for phylogenetic classification, molecular evidence is regarded as significant data or even an essential characteristic in the classification and identification of fungal taxa. In the present study, Neoarthrinium is proposed as a new genus for a group of species phylogenetically distinct from Apiospora, Arthrinium and Nigrospora to maintain monophyletic Arthrinium-like genera. Using morphological and phylogenetic data, however, we need more samples to improve our understanding of Arthrinium-like taxa and genera in the Amphisphaeriales.
This research was funded by the National Microbial Resource Center of the Ministry of Science and Technology of the People’s Republic of China (NMRC-2021-7). The three anonymous reviewers are also acknowledged for their useful comments.