Research Article |
Corresponding author: Lei Cai ( cail@im.ac.cn ) Academic editor: Imke Schmitt
© 2019 Junmin Liang, Guangshuo Li, Shiyue Zhou, Meiqi Zhao, Lei Cai.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Liang J, Li G, Zhou S, Zhao M, Cai L (2019) Myrothecium-like new species from turfgrasses and associated rhizosphere. MycoKeys 51: 29-53. https://doi.org/10.3897/mycokeys.51.31957
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Myrothecium sensu lato includes a group of fungal saprophytes and weak pathogens with a worldwide distribution. Myrothecium s.l. includes 18 genera, such as Myrothecium, Septomyrothecium, Myxospora, all currently included in the family Stachybotryaceae. In this study, we identified 84 myrothecium-like strains isolated from turfgrasses and their rhizosphere. Five new species, i.e., Alfaria poae, Alf. humicola, Dimorphiseta acuta, D. obtusa, and Paramyrothecium sinense, are described based on their morphological and phylogenetic distinctions. Phylogenies were inferred based on the analyses of sequences from four DNA loci (ITS, cmdA, rpb2 and tub2). The generic concept of Dimorphiseta is broadened to include a third type of seta, i.e. thin-walled, straight with obtuse apices.
Stachybotryaceae, soil fungi, turfgrass disease, multi-locus phylogeny, cup-shaped sporodochia
Myrothecium was first introduced by Tode (1790) based on M. inundatum. The typical characters of these fungi are cup-shaped sporodochia covered by a mass of slimy, green to black conidia. The generic concept of Myrothecium has been emended several times (
Most myrothecium-like species are saprobes in soils (
In a survey of turfgrass diseases from 2017, a number of myrothecium-like strains were collected from leaves and roots of turfgrasses and their rhizosphere. The aim of this study was to characterize these strains based on morphology and molecular phylogenetic analyses.
From May 2017 to March 2018, turfgrass diseases were investigated on cold-season species in Beijing and on warm-season species in Hainan Province. Atotal of 130 samples were collected. Each sample was treated as an underground part of soil sample and a ground part of diseased grasses. Soil samples were isolated following the modified dilution plate method (
Species | Isolate no. a | Host/Substrate | Country | NCBI accession numbers | |||
---|---|---|---|---|---|---|---|
cmdA | ITS | tub2 | rpb2 | ||||
Myrothecium simplex |
|
Decaying agaric | Japan | KU846439 | NR145079 | KU846537 | – |
|
Russula nigricans | Japan | KU846440 | KU846457 | KU846538 | – | |
M. inundatum |
|
Russula adusta | England | KU846435 | KU846452 | KU846533 | – |
|
Agaric | Canada | KU846437 | KU846454 | KU846535 | – | |
Albifimbria lateralis | CBS117712T | Unknown | USA | KU845865 | KU845881 | KU845957 | KU845919 |
Alb. terrestris |
|
Soil in mopane woodlands | Namibia | KU845867 | KU845883 | KU845959 | KU845921 |
|
Dead hardwood | USA | KU845866 | KU845882 | KU845958 | KU845920 | |
|
Soil | Namibia | KU845868 | KU845884 | KU845960 | KU845922 | |
LC12196 | rhizosphere soils of Poa sp. | China | MK500260 | MK478879 | MK500277 | – | |
Alb. verrucaria |
|
deteriorated baled cotton | USA | KU845875 | KU845893 | KU845969 | KU845931 |
|
Solanum tubersum | Cyprus | KU845872 | KU845889 | KU845965 | KU845927 | |
LC12191 | Rhizosphere soils of Poa sp. | China | MK500255 | MK478874 | MK500272 | MK500264 | |
LC12192 | Rhizosphere soils of Poa sp. | China | MK500256 | MK478875 | MK500273 | MK500265 | |
LC12193 | Rhizosphere soils of Poa sp. | China | MK500257 | MK478876 | MK500274 | MK500266 | |
LC12194 | Rhizosphere soils of Poa sp. | China | MK500258 | MK478877 | MK500276 | MK500267 | |
LC12195 | Rhizosphere soils of Poa sp. | China | MK500259 | MK478878 | MK500275 | MK500268 | |
Alb. viridis |
|
Unknown | India | KU845879 | KU845898 | KU845974 | KU845936 |
|
Soil | USA | KU845880 | KU845899 | KU845975 | KU845937 | |
Alfaria. ossiformis |
|
Prairie soil | USA | KU845977 | KU845984 | KU846015 | KU846002 |
Alf. humicola sp. nov. | CGMCC3.19213T = LC12143 | Rhizosphere soils of Poa sp. | Beijing, China | MH885432 | MH793291 | MH793317 | MH818829 |
LC12144 | Rhizosphere soils of Poa sp. | Beijing, China | MH885434 | MH793293 | MH793318 | MH818830 | |
Alf. poae sp. nov. | CGMCC3.19198T = LC12140 | Leaves of Poa sp. | Hainan, China | MH885419 | MH793278 | MH793314 | MH818826 |
LC12141 | Rhizosphere soils of Poa sp. | Hainan, China | MH885420 | MH793279 | MH793315 | MH818828 | |
LC12142 | Rhizosphere soils of Poa sp. | Hainan, China | MH885421 | MH793280 | MH793316 | MH818827 | |
Alf. putrefolia |
|
Rotten leaf | Brazil | – | KU845985 | KU846016 | KU846003 |
|
Rotten leaf | Brazil | – | KU845986 | KU846017 | KU846004 | |
Alf. terrestris |
|
Soil | Turkey | KU845979 | KU845988 | KU846019 | KU846006 |
Alf. thymi |
|
Thymus serpyllum | The Netherlands | KU845981 | KU845990 | KU846021 | – |
Capitofimbria compacta |
|
Decaying leaf | Brazil | KU846261 | KU846287 | KU846404 | KU846349 |
|
Bark | Zimbabwe | – | KU878556 | KU878559 | KU878558 | |
Dimorphiseta terrestris |
|
Soil collected in tallgrass prairie | USA | KU846284 | KU846314 | KU846431 | KU846375 |
D. acuta sp. nov. | CGMCC3.19208T = LC12122 | Rhizosphere soils of Poa pratensis | Beijing, China | MH885429 | MH793288 | – | MH818815 |
LC12123 | Leaves of Digitaria sanguinalis | Beijing, China | MH885417 | MH793276 | MH793300 | MH818811 | |
LC12124 | Leaves of Poa pratensis | Beijing, China | MH885418 | MH793277 | MH793297 | MH818812 | |
D. acuta sp. nov. | LC12125 | Rhizosphere soils of Poa pratensis | Beijing, China | MH885427 | MH793286 | MH793298 | MH818813 |
LC12126 | Rhizosphere soils of Poa pratensis | Beijing, China | MH885428 | MH793287 | MH793299 | MH818814 | |
LC12127 | Rhizosphere soils of Poa pratensis | Beijing, China | MH885430 | MH793289 | MH793301 | MH818820 | |
D. obtusa sp. nov. | CGMCC3.19206T = LC12128 | Poa pratensis | Beijing, China | MH885426 | MH793285 | MH793307 | MH818816 |
LC12129 | Rhizosphere soils of Agrostis stolonifera | Beijing, China | MH885415 | MH793274 | MH793303 | MH818821 | |
LC12130 | Rhizosphere soils of Poa pratensis | Beijing, China | MH885431 | MH793290 | MH793308 | MH818817 | |
LC12131 | rhizosphere soils of Poa sp. | Beijing, China | MH885416 | MH793275 | MH793304 | – | |
LC12132 | Rhizosphere soils of Festuca arundinacea | Beijing, China | MH885422 | MH793281 | MH793305 | MH818818 | |
LC12133 | Rhizosphere soils of Poa pratensis | Beijing, China | MH885423 | MH793282 | MH793306 | MH818819 | |
LC12134 | Roots of Poa pratensis | Beijing, China | MH885424 | MH793283 | MH793309 | – | |
LC12135 | Roots of Poa pratensis | Beijing, China | MH885425 | MH793284 | MH793302 | – | |
Gregatothecium humicola |
|
Soil | Papua New Guinea | KU846285 | KU846315 | KU846432 | KU846376 |
Peethambara sundara |
|
Dead twig | India | – | KU846471 | KU846551 | KU846509 |
|
Dead twig | Nepal | – | KU846470 | KU846550 | KU846508 | |
Inaequalispora prestonii |
|
Forest soil | Malaysia | KU846286 | KU846316 | KU846433 | KU846377 |
|
rhizoplane and roots of plants | Ecuador | – | KY389317 | KY366447 | KY389355 | |
Myxospora masonii |
|
Leaves of Glyceria sp. | England | KU846445 | KU846462 | KU846543 | KU846500 |
My. graminicola |
|
Decaying grass leaf | USA | KU846444 | KU846461 | KU846542 | KU846499 |
My. aptrootii |
|
Leaf litter | China | KU846441 | KU846458 | KU846539 | KU846496 |
My. musae |
|
Musa sp. | Madagascar | – | KU846463 | KU846544 | KU846501 |
CPC 25150 | Tarspot lesion | South Africa | KU846446 | KU846464 | KU846545 | KU846502 | |
My. crassiseta |
|
Dead twig | Japan | KU846442 | KU846459 | KU846540 | KU846497 |
|
Pyrenomycete | Hawaii | KU846443 | KU846460 | KU846541 | KU846498 | |
Paramyrothecium humicola |
|
Soil collected in tallgrass prairie | USA | – | KU846295 | KU846412 | KU846356 |
P. parvum |
|
Viola sp. | United Kingdom | – | KU846298 | KU846415 | KU846359 |
|
Dune sand | France | KU846268 | KU846297 | KU846414 | KU846358 | |
P. foeniculicola |
|
Foeniculum vulgare leaf sheath | The Netherlands | – | KU846292 | KU846409 | KU846354 |
P. nigrum |
|
Soil | Spain | KU846267 | KU846296 | KU846413 | KU846357 |
LC12188 | Rhizosphere soils of Poa sp. | China | MK500252 | MK478871 | MK500269 | MK500261 | |
P. cupuliforme |
|
Surface soil in desert | Namibia | KU846264 | KU846291 | KU846408 | KU846353 |
P. viridisporum |
|
Soil | Turkey | KU846278 | KU846308 | KU846425 | KU846369 |
|
Soil | USA | KU846280 | KU846310 | KU846427 | KU846371 | |
P. acadiense |
|
Tussilago farfara | Canada | – | KU846288 | KU846405 | KU846350 |
P. terrestris |
|
Soil | Turkey | KU846273 | KU846303 | KU846420 | KU846364 |
|
Soil | Turkey | KU846275 | KU846305 | KU846422 | KU846366 | |
P. tellicola |
|
Soil | Turkey | KU846272 | KU846302 | KU846419 | KU846363 |
P. foliicola |
|
Decaying leaf | Brazil | KU846266 | KU846294 | KU846411 | – |
|
Air | Cuba | KU846265 | KU846293 | KU846410 | KU846355 | |
P. breviseta |
|
Unknown | India | KU846262 | KU846289 | KU846406 | KU846351 |
P. roridum |
|
Gardenia sp. | Italy | KU846270 | KU846300 | KU846417 | KU846361 |
|
Water | The Netherlands | KU846269 | KU846299 | KU846416 | KU846360 | |
|
Coffea sp. | Colombia | KU846271 | KU846301 | KU846418 | KU846362 | |
P. guiyangense | GUCC 201608S01T | Soil | Guiyang, China | KY196193 | KY126418 | KY196201 | – |
HGUP 2016-8001 | Soil | Guiyang, China | KY196192 | KY126417 | KY196200 | – | |
P. verruridum | |||||||
HGUP 2016-8006T | Soil | Guizhou, China | KY196197 | KY126422 | KY196205 | – | |
P. sinense sp. nov. | CGMCC3.19212T = LC12136 | Rhizosphere soils of Poa sp. | Beijing, China | MH885437 | MH793296 | MH793313 | MH818824 |
LC12137 | Rhizosphere soils of Poa sp. | Beijing, China | MH885436 | MH793295 | MH793312 | MH818822 | |
LC12138 | Rhizosphere soils of Poa sp. | Beijing, China | MH885433 | MH793292 | MH793310 | MH818823 | |
LC12139 | Rhizosphere soils of Poa sp. | Beijing, China | MH885435 | MH793294 | MH793311 | MH818825 | |
Parvothecium terrestre |
|
Soil in virgin forest | Brazil | KU846449 | KU846468 | KU846548 | KU846506 |
Neomyrothecium humicola |
|
Soil | Papua New Guinea | KU846448 | KU846467 | – | KU846505 |
Gregatothecium humicola |
|
Soi | Papua New Guinea | KU846285 | KU846315 | KU846432 | KU846376 |
Xepicula crassiseta |
|
Soil | Spain | KU847222 | KU847247 | KU847337 | KU847296 |
X. jollymannii |
|
Nicotiana tabacum | Malawi | KU847223 | KU847248 | KU847338 | KU847297 |
|
Soil | Namibia | KU847224 | KU847250 | KU847340 | KU847298 | |
X. leucotricha |
|
Soil | India | KU847225 | KU847251 | KU847341 | KU847299 |
|
Soil | Colombia | KU847228 | KU847254 | KU847344 | KU847302 | |
Smaragdiniseta bisetosa |
|
Rotten bark | India | KU847206 | KU847229 | KU847319 | KU847281 |
Striaticonidium brachysporum |
|
Dune sand | Iran | KU847209 | KU847232 | KU847322 | KU847284 |
S. brachysporum |
|
Soil | Ukrain | KU847207 | KU847230 | KU847320 | KU847282 |
LC12189 | Rhizosphere soils of Poa sp. | Beijing, China | MK500253 | MK478872 | MK500270 | MK500262 | |
LC12190 | Rhizosphere soils of Poa sp. | Beijing, China | MK500254 | MK478873 | MK500271 | MK500263 | |
S. synnematum |
|
Palm leaf | Japan | KU847218 | KU847242 | KU847332 | KU847292 |
S. cinctum |
|
Soil | The Netherlands | KU847216 | KU847239 | KU847329 | KU847290 |
|
Soil | New Zealand | KU847213 | KU847236 | KU847326 | KU847288 | |
S. humicola |
|
Soil | Papua New Guinea | KU847217 | KU847241 | KU847331 | KU847291 |
Tangerinosporium thalictricola |
|
Thalictrum flavum | UK | KU847219 | KU847243 | KU847333 | – |
Xenomyrothecium tongaense |
|
Halimeda sp. | Tonga | KU847221 | KU847246 | KU847336 | KU847295 |
Virgatospora echinofibrosa |
|
Theobroma cacao | Ecuador | KU847220 | KU847244 | KU847334 | KU847293 |
|
Trewia nudiflora | Nepal | – | KU847245 | KU847335 | KU847294 | |
Fusarium sambucinum |
|
Solanum tuberosum | UK | KM231391 | KM231813 | KM232078 | KM232381 |
Descriptions of macromorphological features are based on 7-d old materials incubated in the dark at room temperature (20–25 °C) and grown on potato dextrose agar (2% w/w; PDA), oatmeal agar (OA), cornmeal agar (CMA) and synthetic low-nutrient agar (SNA;
Genomic DNA was extracted from 1–2 weeks’ old cultures grown on potato dextrose agar (2% w/w; PDA) incubated at room temperature using a modified Cetyltrimethyl Ammonium Bromide (CTAB) method (
The purified PCR products were sequenced in both forward and reverse directions on an ABI-3730 XL DNA Analyzer (Applied Biosystems, California, USA). The sequences were checked and manually corrected where necessary. A consensus contig was assembled with BioEdit v. 7.0.9 (
Phylogenetic analyses were based on Bayesian inference (BI) and Maximum Likelihood (ML). For BI analysis, the optimal evolutionary model was estimated in MrModeltest v. 2.3 (
In this study, 603 fungal strains were isolated. Based on colony morphologies and preliminary sequence comparison of ITS via BLASTn in GenBank, 84 myrothecium-like strains were selected. Phylogenetic analyses of above 84 strains were performed on single locus and concatenated datasets (ITS, cmdA, tub2 and rpb2), with 70 strains in Myrothecium s.l. as reference and Fusarium sambucinum (
The ML consensus tree inferred from a four-locus concatenated alignment (ITS, cmdA, rpb2 and tub2). Bootstrap values (1,000 replicates) over 70% for ML and posterior probability (PP) over 0.95 are added to the left of a node (ML/PP). The type strains are labeled with “T”. Strains obtained from this study are in red. The tree is rooted using Fusarium sambucinum (
Characteristics of the different datasets and statistics of phylogenetic analyses used in this study.
Locus† | Number of sites* | Evolutionary model‡ | Number of tree sampled in B | Maximum-likelihood statistics | ||||
---|---|---|---|---|---|---|---|---|
Total | Conserved | Phylogenetically informative | B unique patterns | Best tree optimised likelihood | Tree length | |||
ITS | 569 | 334 | 193 | 247 | GTR+I+G | 7501 | -32666.73 | 5.36 |
tub2 | 318 | 168 | 140 | 159 | HKY+I+G | |||
cmdA | 732 | 258 | 381 | 490 | HKY+I+G | |||
rpb2 | 724 | 360 | 367 | 367 | GTR+I+G |
Dimorphiseta terrestris L. Lombard & Crous. Persoonia. 36: 188. 2016. (Type species)
Dimorphiseta was a monotypic genus, introduced based on D. terrestris, which showed both type I (thin-walled, flexuous to circinate, narrowing to a sharp apex) and type II (thick-walled, straight to slightly curved, narrowing to a sharp apex) setae. Our study demonstrated that there is a third type of setae (type III: thin-walled, straight, terminating in an obtuse apex) in the genus.
China, Beijing, isolated from rhizosphere soils of Poa pratensis, 26 Aug 2017, J.M. Liang, holotype HMAS 247957, dried culture on PDA, ex-holotype culture CGMCC3.19208 = LC12122.
Colonies on PDA, CMA and OA approx. 7–8 cm diam. after 7 d at room temperature (approx. 25 °C), mycelium white and abundant, with conidiophores forming on the aerial mycelium, carrying slimy olivaceous green to black conidial masses, reverse on PDA buff. Conidiomata sporodochial, stromatic, superficial, cupulate to discoid, scattered, rarely gregarious, irregular in outline, 50–300 μm diam., 60–150 μm deep, consisting of bundles of parallel, longitudinal, closely compacted hyphae, terminating in whorls of 3–5 conidiogenous cells, covered by an olivaceous green to black slimy mass of conidia without marginal hyphae. Stroma poorly developed, hyaline, of a textura angularis. Setae arising from the conidial mass, thick-walled, subhyaline, smooth, 5–15-septate, tapering to sharp apices, 120–370 μm long, 10–13 μm wide at the broadest part, 2–4 μm wide at the apex. Conidiophores macronematous, irregularly, unbranched, smooth to lightly verrucose, arising from the basal stroma. Conidiogenous cells phialidic, subcylindrical, hyaline, smooth, 10–20 μm long, 2–3 μm wide. Conidia aseptate, smooth, hyaline, ellipsoidal, rounded at the base, pointed at the apex with a funnel-shaped appendage, 7–12 × 2–3 μm (av. 10 ± 0.7 × 3 ± 1.3 μm, n = 50).
China.
Name refers to the setae with tapered and sharp apices.
China, Beijing, from leaves of Digitaria sanguinalis, 21 Aug 2017, J.M. Liang, LC12123; China, Beijing, from leaves of Poa pratensis, 21 Aug 2017, J.M. Liang, LC12124; China, Beijing, from rhizosphere soils of P. pratensis, 21 Aug 2017, J.M. Liang & G.S. Li, LC12125, 21 Jul 2017, J.M. Liang, LC12126, 25 Jul 2017, J.M. Liang, LC12127.
The multi-locus phylogenetic analyses indicated that D. acuta formed a sister clade to D. terrestris, but differs from the latter in the type and size of setae. Dimorphiseta terrestris produces both types of setae, the thin-walled and circinate type (Type I) and the thick-walled sharp-edged type (Type II), whereas D. acuta only produces the type I setae. In addition, the setae of D. acuta are much longer and wider than that in D. terrestris (120–370 μm × 10–13 μm vs. 70–95 × 3–4 μm) (
China, Beijing, isolated from rhizosphere soils of P. pratensis, 23 Jun 2017, J.M. Liang, holotype HMAS 247954, ex-holotype culture CGMCC3.19206 = LC12128.
Colonies on PDA, OA and CMA approx. 5–6 cm diam. after 7 d at room temperature (approx. 25 °C), mycelium white and abundant, with conidiophores forming on the aerial mycelium, carrying slimy olivaceous green to black conidial masses, reverse on PDA pale luteous to buff. Conidiomata sporodochial, stromatic, superficial, scattered, rarely gregarious, oval to elongate or irregular in outline, 60–280 µm diam., 40–120 µm deep, with a setose fringe surrounding green to black slimy mass of conidia. Stroma poorly developed, hyaline, smooth to verrucose, of textura angularis. Setae arising from the basal stroma, thin-walled, 3–6-septate, unbranched, hyaline, smooth, 80–250 µm long, 2–4 µm wide at the broadest, terminating in a blunt apex. Conidiophores macronematous, irregularly, unbranched, smooth to lightly verrucose, arising from the basal stroma, up to 18 μm long. Conidiogenous cells phialidic, hyaline, smooth to verrucose, cylindrical, 7–19 × 2–3 μm, becoming narrowed at the tip with collarette. Conidia aseptate, ellipsoidal or cylindrical, hyaline, smooth, rounded both ends, with a funnel-shaped apical appendage, 9–11 × 2–4 μm (av. 10 ± 0.5 × 3 ± 0.3 μm, n = 50).
China.
Named refers the setae with obtuse apices.
China, Beijing, from rhizosphere soils of Agrostis stolonifera, 24 Jul 2017, J.M. Liang, LC12129; China, Beijing, from rhizosphere soils of P. pratensis, 25 Aug 2017, J.M. Liang & G.S. Li, LC12130, 19 Jul 2017, J.M. Liang, LC12133; China, Beijing, from rhizosphere soils of Poa sp., 19 Jul 2017, J.M. Liang, LC12131; China, Beijing, from rhizosphere soils of Festuca arundinacea, 19 Jul 2017, J.M. Liang, LC12132; China, Beijing, from leaves of P. pratensis, 23 Jun 2017, J.M. Liang, LC12134, LC12135.
Dimorphiseta obtusa formed a highly supported cluster with D. terrestris and D. acuta, but can be distinguished from the latter two by having setae with erect and obtuse apices. In addition, D. obtusa is also morphologically similar to two old un-sequenced Myrothecium taxa, i.e. M. biforme and M. dimorphum, but both of these two taxa have two types of conidia. Myrothecium biforme produces short cylindrical and ellipsoidal to navicular conidia (
China, Beijing, Olympic Park, from rhizosphere soil of Poa sp., 13 Dec 2017, S.Y. Zhou, holotype HMAS 247955, ex-holotype culture CGMCC3.19213 = LC12143.
Colonies on PDA, CMA and OA approx. 7–8 cm diam. after 7 d at 25 °C. Hyphae hyaline, smooth, branched, 1–2 μm wide. Conidiomata sporodochial, stromatic, superficial, cupulate to discoid, scattered to gregarious, oval to elongate or irregular in outline, 50–200 μm diam., 70–150 μm deep, without setose hyphae, covered by a green to black agglutinated slimy mass of conidia. Stroma well-developed, hyaline, of textura globulose or textura angularis. Setae absent. Conidiophores arising from the basal stroma, unbranched or branched, initially hyaline and smooth, becoming pigmented and verrucose with age, 11–25 µm long. Conidiogenous cells phialidic, cylindrical to allantoid, initially hyaline and smooth becoming pigmented and verrucose with age, 14–33 × 2–3 µm. Conidia aseptate, smooth, hyaline, elongated ellipsoidal to limoniform, straight, 7–9(–10) × 2–3 µm (av. 8 ± 0.6 × 3 ± 0.2 µm, n = 50).
China.
Name refers the substrate, soil, from which this fungus was isolated.
China, Beijing, Olympic Park, from rhizosphere soil of Poa sp., 13 Dec 2017, S.Y. Zhou, LC12144.
Alfaria humicola represents another distinct lineage in Alfaria (Fig.
China, Hainan Province, Haikou, isolated from leaves of Imperata cylindrica, 10 Mar 2018, J.M. Liang and L. Cai, holotype HMAS 247953, ex-holotype culture CGMCC3.19198 = LC12140.
Colonies on PDA, CMA and OA with white aerial mycelium, approx. 6–7 cm diam. after 7 d at 25 °C, giving rise to dark green or blank sporodochia scattered or gregarious on the surface, covered by olivaceous green pillars of conidia, reverse on PDA sienna. Hyphae hyaline, smooth, branched, 1–2 μm wide. Conidiomata synnematous, solitary, 60–250 μm high, 30–80 μm wide at the base, 60–150 μm at the apex, with setose hyphae surrounding a green agglutinated mass of conidia. Stroma well developed, hyaline, of textura angularis. Setae absent. Conidiophores arising from the basal stroma, branched, initially hyaline and becoming pigmented and verrucose with age covered by an olivaceous green mucoid layer, up to 30 µm long. Conidiogenous cell phialidic, clavate to cylindrical, hyaline, smooth, 5–10 × 1–2 µm, becoming pigmented and verrucose with age, with conspicuous collarettes and periclinal thickenings. Conidia aseptate, smooth, hyaline, ellipsoidal to fusiform, 6–8 × 2–3 µm (av. 7 ± 0.4× 2 ± 0.2 µm, n = 50).
China.
Name refers the host, Poa sp., from which this fungus was isolated.
China, Hainan, from leaves of Imperata cylindrica, 10 Mar 2018, J.M. Liang & Lei Cai, LC12141, LC12142.
Alfaria poae formed a well-supported clade in Alfaria (Fig.
China, Beijing, Olympic Park, from rhizosphere soil of Poa sp., 13 Dec 2017, S.Y. Zhou, holotype HMAS 247956, ex-holotype culture CGMCC3.19212 = LC12136.
Colonies on PDA, CMA and OA approx. 5–6 cm diam. after 7 d at 25 °C. Hyphae white, hyaline, smooth, branched, 1–2 μm wide, reverse on PDA pale luteous. Conidiomata sporodochial, stromatic, cupulate, superficial, scattered or gregarious, oval or irregular in outline, 80–600 μm diam., 50–150 μm deep, with a white setose fringe surrounding an olivaceous green to black agglutinated slimy mass of conidia. Stroma poorly developed, hyaline, of textura angularis. Setae arising from stroma, thin-walled, hyaline, 1–3-septate, straight to flexuous, 45–90 μm long, 1–3 μm wide, tapering to an acutely rounded apex. Conidiophores arising from the basal stroma, consisting of a stipe and a penicillately branched conidiogenous apparatus; stipes unbranched, hyaline, septate, smooth, 20–30 × 2–3 μm; primary branches aseptate, unbranched, smooth, 13–40 × 2–3 μm; secondary branches aseptate, unbranched, smooth, 8–15 × 2–3 μm; terminating in a whorl of 3–6 conidiogenous cells; conidiogenous cell phialidic, cylindrical to subcylindrical, hyaline, smooth, straight to slightly curved, 7–16 × 1–3 μm, with conspicuous collarettes and periclinal thickenings. Conidia aseptate, hyaline, smooth, cylindrical, 6–7 × 2–3 μm (av. 7 ± 0.3 × 2 ± 0.2 μm, n = 40), rounded at both ends.
China.
Named after the country of collection, China.
China, Beijing, Olympic Park, from rhizosphere soils of Poa sp., 13 Dec 2017, S.Y. Zhou, LC12137, LC12138, LC12139.
Paramyrothecium sinense formed a highly supported distinct clade closely related to P. humicola. The setae of this species are terminated with obtuse apices, dissimilar to the acute apices in P. humicola. In addition, the conidiophore stipes (20–30 μm long) and primary branches (13–40 μm long) of P. sinense are much longer than those of P. humicola (stipe, 12–22 μm long; primary branches, 7–17 μm long) (
The ITS has been shown to be insufficient to delineate the myrothecium-like species. With the additions of partial sequences of rpb2, cmdA and tub2, phylogenetic relationships within Stachybotryaceae could be better resolved (
By comparing the topologies of the four single-locus trees, incomplete lineage sorting was discovered in Dimorphiseta. Based on the single-locus trees of ITS and rpb2, D. acuta, D. obtusa and D. terrestris grouped together (Supp. materials
In the multi-locus sequence analysis of Myrothecium s.l. by
This study was financially supported by National Natural Science Foundation of China (NSFC 31600405).
Figure S1. The ML consensus tree inferred based on ITS partial sequence with bootstrap values for ML (> 70%) and posterior probability (PP) (PP > 0.95) labeled to the left of a node (ML/PP)
Data type: phylogenetic data
Explanation note: The type strains were labeled with “T”. Strains obtained from this study are in red.
Figure S2. The ML consensus tree inferred based on tub2 partial sequence with bootstrap values for ML (> 70%) and posterior probability (PP) (PP > 0.95) labeled to the left of a node (ML/PP)
Data type: phylogenetic data
Explanation note: The type strains were labeled with “T”. Strains obtained from this study are in red.
Figure S3. The ML consensus tree inferred based on cmdA partial sequence with bootstrap values for ML (> 70%) and posterior probability (PP) (PP > 0.95) labeled to the left of a node (ML/PP)
Data type: phylogenetic data
Explanation note: The type strains were labeled with “T”. Strains obtained from this study are in red.
Figure S4. The ML consensus tree inferred based on rpb2 partial sequence with bootstrap values for ML (> 70%) and posterior probability (PP) (PP > 0.95) labeled to the left of a node (ML/PP)
Data type: phylogenetic data
Explanation note: The type strains were labeled with “T”. Strains obtained from this study are in red.
Figure S5. The ML consensus tree inferred based on LSU and rpb2 partial sequences with bootstrap values for ML (> 70%) and posterior probability (PP) (PP > 0.95) labeled to the left of a node (ML/PP)
Data type: phylogenetic data
Explanation note: The type strains were labeled with “T”. Strains obtained from this study are in red.
Table S1. NCBI GenBank accessions of 28S ribosomal DNA large-subunit sequences (LSU) used in the phylogenetic analyses
Data type: phylogenetic data