New species in Dictyosporium, new combinations in Dictyocheirospora and an updated backbone tree for Dictyosporiaceae

Jing Yang; Jian-Kui Liu; Kevin D. Hyde; E.B. Gareth Jones; Zuo-Yi Liu

doi:10.3897/mycokeys.36.27051

Research Article

New species in Dictyosporium, new combinations in Dictyocheirospora and an updated backbone tree for Dictyosporiaceae

Jing Yang^‡§, Jian-Kui Liu^‡, Kevin D. Hyde^|§¶, E.B. Gareth Jones^#, Zuo-Yi Liu^‡

‡ Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang, China

§ Mae Fah Luang University, Chiang Rai, Thailand

| World Agroforestry Centre, Kunming, China

¶ Kunming Institute of Botany, Chinese Academy of Science, Kunming, China

# Chiang Mai University, Chiang Mai, Thailand

Corresponding author: Jing Yang ( yangjing5633@gmail.com )

Academic editor: Paul Kirk

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Yang J, Liu JK, Hyde KD, Jones EBG, Liu ZY (2018) New species in Dictyosporium, new combinations in Dictyocheirospora and an updated backbone tree for Dictyosporiaceae. MycoKeys 36: 83-105. https://doi.org/10.3897/mycokeys.36.27051

Abstract

A survey of freshwater fungi on submerged wood in China and Thailand resulted in the collection of three species in Dictyocheirospora and four species in Dictyosporium including two new species in the latter genus. Morphological characters and phylogenetic analyses based on ITS, LSU and TEF1α sequence data support their placement in Dictyocheirospora and Dictyosporium (Dictyosporiaceae). An updated backbone tree is provided for the family Dictyosporiaceae. Descriptions and illustrations of the new taxa and re-collections are provided. Four new combinations are proposed for Dictyocheirospora.

Keywords

2 new taxa, asexual morph, Dothideomycetes , phylogeny, taxonomy

Introduction

The family Dictyosporiaceae was introduced by Boonmee et al. (2016) to accommodate mostly aquatic lignicolous species with cheiroid, digitate, palmate and/or dictyosporous conidia and their sexual morphs that form a monophyletic clade in the class Dothideomycetes.

Dictyosporium, the type genus of the family, has been reported worldwide from dead wood and plant litter in terrestrial and aquatic habitats (Hyde and Goh 1998, Ho et al. 2002, Pinnoi et al. 2006, Pinruan et al. 2007). Corda (1836) established the genus with D. elegans Corda as the type species. The holomorph genus is characterised by dark brown, subglobose superficial ascomata, bitunicate cylindrical asci and hyaline, fusiform uniseptate ascospores with or without a sheath; sporodochial colonies, micronematous to macronematous conidiophores and cheiroid, digitate complanate conidia with several parallel rows of cells. Goh et al. (1999) reviewed the genus accepting 22 species and the remaining 16 species were doubtful or excluded. Tsui et al. (2006) first considered that the genus is closely related to Massarinaceae (Pleosporales) based on phylogenetic analysis using SSU and LSU sequence data. Tanaka et al. (2015) and Boonmee et al. (2016) confirmed the phylogenetic placement of Dictyosporium in Dictyosporiaceae (Massarineae, Pleosporales). Recent comparisons of Dictyosporium species were provided by Whitton et al. (2012), Prasher and Verma (2015) and Silva et al. (2015) with up to 48 accepted species. Since Silva et al. (2015), D. araucariae S.S. Silva, R.F. Castañeda & Gusmão, D. hydei I.B. Prasher & R.K. Verma, D. indicum I.B. Prasher & R.K. Verma, D. olivaceosporum Kaz. Tanaka, K. Hiray., Boonmee & K.D. Hyde, D. palmae Abdel-Aziz, D. pseudomusae Kaz. Tanaka, G. Sato & K. Hiray., D. sexualis Boonmee & K.D. Hyde, D. splendidum Alves-Barb., Malosso & R.F. Castañeda and D. wuyiense Y. Zhang & G.Z. Zhao were newly introduced to the genus (Prasher and Verma 2015, Tanaka et al. 2015, Abdel-Aziz 2016, Boonmee et al. 2016, da Silva et al. 2016, Alves-Barbosa et al. 2017, Zhang et al. 2017) and nine species were re-assigned to Dictyocheirospora, Jalapriya and Vikalpa (Boonmee et al. 2016). Wijayawardene et al. (2017a) provided information on the availability of cultures and references to accessible sequence data.

Dictyocheirospora was introduced by Boonmee et al. (2016) with Di. rotunda D’souza, Bhat & K.D. Hyde as the type species. Dictyocheirospora is morphologically similar to Dictyosporium except in having cheiroid, non-complanate or cylindrical conidia, mostly with conidial arms closely gathered together at the apex. Ten species are accepted in the genus including four species transferred from Dictyosporium (Boonmee et al. 2016, Wang et al. 2016, Hyde et al. 2017, Li et al. 2017).

During a survey of freshwater fungi on submerged wood along a north/south gradient in the Asian/Australasian region (Hyde et al. 2016), two new freshwater species and five previously described species were collected and identified based on phylogenetic analyses and morphological characters. We therefore introduce Dictyosporium tubulatum and Dictyosporium tratense as new species, with an illustrated account and phylogenetic evidence for the new taxa. An updated backbone tree based on the combined ITS, LSU and TEF1α sequence data is provided for Dictyosporiaceae. Four new combinations are proposed in Dictyocheirospora.

Materials and methods

Collection and examination of specimens

Specimens of submerged, decaying wood were collected from streams in Chiang Rai, Prachuap Khiri Khan, Phang Nga and Trat Provinces, Thailand, in December 2014, 2015, April 2016 and Guizhou Province, China, in October 2016. Specimens were brought to the laboratory in plastic bags and incubated in plastic boxes lined with moistened tissue paper at room temperature for one week. Morphological observations were made using a Motic SMZ 168 Series dissecting microscope for fungal structures on natural substrate. The fungal structures were collected using a syringe needle and transferred to a small drop of distilled water on a clean slide and covered with a cover glass. The fungi were examined using a Nikon ECLIPSE 80i compound microscope and photographed with a Canon 550D, 600D or 70D digital camera fitted to the microscope. Measurements were made with the TAROSOFT (R) IMAGE FRAME WORK programme and images used for figures were processed with ADOBE PHOTOSHOP CS6 software. Single spore isolations were made on to potato dextrose agar (PDA) or water agar (WA) and later transferred on to malt extract agar (MEA) or PDA following the method of Chomnunti et al. (2014). Specimens (dry wood with fungal material) are deposited in the herbarium of Mae Fah Luang University (MFLU), Chiang Rai, Thailand and Kunming Institute of Botany, Academia Sinica (HKAS), China. Axenic cultures are deposited in Mae Fah Luang University Culture Collection (MFLUCC). Facesoffungi and Index Fungorum numbers are registered as outlined in Jayasiri et al. (2015) and Index Fungorum (2018).

DNA extraction, PCR amplification and sequencing

Isolates were grown on PDA and/or MEA medium at 25 °C for one month. Fungal mycelium was scraped off and transferred to a 1.5-ml microcentrifuge tube using a sterilised lancet for genomic DNA extraction. Ezup Column Fungi Genomic DNA Purification Kit (Sangon Biotech, China) was used to extract DNA following the manufacturer’s instructions. ITS, LSU and TEF1α gene regions were amplified using the primer pairs ITS5 or ITS1 with ITS4 (Vilgalys and Hester 1990), LROR with LR5 or LR7 (White et al. 1990) and EF1-983F with EF1-2218R (Rehner 2001). The amplifications were performed in a 25 μl reaction volume containing 9.5 μl ddH₂O, 12.5 μl 2 × Taq PCR Master Mix with blue dye (Sangon Biotech, China), 1 μl of DNA template and 1 μl of each primer (10 μM). The amplification condition for ITS, LSU and TEF1α consisted of initial denaturation at 94 °C for 3 min; followed by 40 cycles of 45 s at 94 °C, 50 s at 56 °C and 1 min at 72 °C and a final extension period of 10 min at 72 °C. Purification and sequencing of PCR products were carried out using the above-mentioned PCR primers at Sangon Biotech (Shanghai) Co. Ltd. in China.

Phylogenetic analyses

The taxa included in the phylogenetic analyses were selected and obtained from previous studies and GenBank (Boonmee et al. 2016, Wang et al. 2016, Li et al. 2017). Three gene regions (ITS, LSU and TEF1α) were used for the combined sequence data analyses. SEQMAN v. 7.0.0 (DNASTAR, Madison, WI) was used to assemble consensus sequences. The sequences were aligned using the online multiple alignment programme MAFFT v.7 (http://mafft.cbrc.jp/alignment/server/) (Katoh and Standley 2013). The alignments were checked visually and improved manually where necessary.

Phylogenetic analysis of the sequence data consisted of maximum likelihood (ML) using RAxML-HPC v.8 (Stamatakis 2006, Stamatakis et al. 2008) on the XSEDE Teragrid of the CIPRES science Gateway (https://www.phylo.org) (Miller et al. 2010) with rapid bootstrap analysis, followed by 1000 bootstrap replicates. The final tree was selected amongst suboptimal trees from each run by comparing likelihood scores under the GTRGAMMA substitution model.

Maximum parsimony (MP) analyses were performed with PAUP v. 4.0b10 (Swofford 2003) using the heuristic search option with 1000 random taxa addition and tree bisection and reconnection (TBR) as the branch swapping algorithm. All characters were unordered and of equal weight and gaps were treated as missing data. Maxtrees were unlimited, branches of zero length were collapsed and all multiple, equally parsimonious trees were saved. Clade stability was assessed using a bootstrap (BT) analysis with 1000 replicates, each with 10 replicates of random stepwise addition of taxa (Hillis and Bull 1993).

The programme MRMODELTEST2 v. 2.3 (Nylander 2008) was used to infer the appropriate substitution model that would best fit the model of DNA evolution for the combined datasets for Bayesian inference analysis with GTR+G+I substitution model selected. Posterior probabilities (PP) (Rannala and Yang 1996, Zhaxybayeva and Gogarten 2002) were determined by Markov Chain Monte Carlo sampling (MCMC) in MRBAYES v. 3.0b4 (Huelsenbeck and Ronquist 2001). Six simultaneous Markov chains were run for 1 million generations, with trees sampled every 100 generations (resulting in 10000 trees). The first 2000 trees, representing the burn-in phase of the analyses were discarded and the remaining 8000 trees were used for calculating posterior probabilities (PP) in the majority rule consensus tree (Larget and Simon 1999).

The resulting trees were printed with FIGTREE v. 1.4.0 (http://tree.bio.ed.ac.uk/software/figtree/) and the layout was created in MICROSOFT POWERPOINT for Mac v. 15.19.1. The alignment of phylogenetic analyses and resultant tree were deposited in TreeBASE (www.treebase.org, submission number 22802). Sequences generated in this study were submitted to GenBank (Table 1).

Table 1.

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Isolates and sequences used in this study (newly generated sequences are indicated in bold, ex-type strains are indicated with ^T after strain number).

Species	Source	GenBank accession number
Species	Source	ITS	LSU	TEF1α
Aquadictyospora lignicola	MFLUCC 17-1318^T	MF948621	MF948629	MF953164
Aquaticheirospora lignicola	HKUCC 10304^T	AY864770	AY736378	–
Cheirosporium triseriale	HMAS 180703^T	EU413953	EU413954	–
Dendryphiella eucalyptorum	CBS 137987^T	KJ869139	KJ869196	–
Dendryphiella fasciculata	MFLUCC 17-1074^T	MF399213	MF399214	–
Dendryphiella paravinosa	CBS 141286^T	KX228257	KX228309	–
Dictyocheirospora aquatica	KUMCC 15-0305^T	KY320508	KY320513	–
Dictyocheirospora bannica	KH 332^T	LC014543	AB807513	AB808489
*Dictyocheirospora bannica*	MFLUCC 16-0874	MH381765	MH381774	–
Dictyocheirospora garethjonesii	MFLUCC 16-0909^T	KY320509	KY320514	–
Dictyocheirospora garethjonesii	DLUCC 0848	MF948623	MF948631	MF953166
Dictyocheirospora gigantica	BCC 11346	DQ018095	–	–
Dictyocheirospora heptaspora	CBS 396.59	DQ018090	–	–
*Dictyocheirospora indica*	MFLUCC 15-0056	MH381763	MH381772	MH388817
Dictyocheirospora pseudomusae	yone 234^T	LC014550	AB807520	AB808496
Dictyocheirospora rotunda	MFLUCC 14-02⁹3T	KU179099	KU179100	–
*Dictyocheirospora rotunda*	MFLUCC 17-0222	MH381764	MH381773	MH388818
Dictyocheirospora rotunda	MFLUCC 17-1313	MF948625	MF948633	MF953168
Dictyocheirospora subramanianii	BCC 3503	DQ018094	–	–
Dictyocheirospora vinaya	MFLUCC 14-0294^T	KU179102	KU179103	–
Dictyosporium alatum	ATCC 34953^T	NR_077171	DQ018101	–
Dictyosporium aquaticum	MF 1318^T	KM610236	–	–
Dictyosporium bulbosum	yone 221	LC014544	AB807511	AB808487
Dictyosporium digitatum	KH 401	LC014545	AB807515	AB808491
Dictyosporium digitatum	yone 280	LC014547	AB807512	AB808488
Dictyosporium elegans	NBRC 32502^T	DQ018087	DQ018100	–
Dictyosporium hughesii	KT 1847	LC014548	AB807517	AB808493
Dictyosporium meiosporum	MFLUCC 10-0131^T	KP710944	KP710945	–
Dictyosporium nigroapice	BCC 3555	DQ018085	–	–
*Dictyosporium nigroapice*	MFLUCC 17-2053	MH381768	MH381777	MH388821
Dictyosporium olivaceosporum	KH 375^T	LC014542	AB807514	AB808490
Dictyosporium sexualis	MFLUCC 10-0127^T	KU179105	KU179106	–
Dictyosporium sp.	MFLUCC 15-0629	MH381766	MH381775	MH388819
Dictyosporium stellatum	CCFC 241241^T	NR_154608	JF951177	–
Dictyosporium strelitziae	CBS 123359^T	NR_156216	FJ839653	–
Dictyosporium tetrasporum	KT 2865	LC014551	AB807519	AB808495
Dictyosporium thailandicum	MFLUCC 13-0773^T	KP716706	KP716707	–
*Dictyosporium tratense*	MFLUCC 17-2052^T	MH381767	MH381776	MH388820
*Dictyosporium tubulatum*	MFLUCC 15-0631^T	MH381769	MH381778	MH388822
*Dictyosporium tubulatum*	MFLUCC 17-2056	MH381770	MH381779	–
Dictyosporium wuyiense	CGMCC 3.18703^T	KY072977	–	–
Dictyosporium zhejiangense	MW-2009a^T	FJ456893	–	–
Digitodesmium bambusicola	CBS 110279^T	DQ018091	DQ018103	–
Gregarithecium curvisporum	KT 922^T	AB809644	AB807547	–
Jalapriya inflata	NTOU 3855	JQ267362	JQ267363	–
Jalapriya pulchra	MFLUCC 15-0348^T	KU179108	KU179109	–
Jalapriya pulchra	MFLUCC 17-1683	MF948628	MF948636	MF953171
Jalapriya toruloides	CBS 209.65	DQ018093	DQ018104	–
Periconia igniaria	CBS 379.86	LC014585	AB807566	AB808542
Periconia igniaria	CBS 845.96	LC014586	AB807567	AB808543
Pseudocoleophoma calamagrostidis	KT 3284^T	LC014592	LC014609	LC014614
Pseudocoleophoma polygonicola	KT 731^T	AB809634	AB807546	AB808522
Pseudocoleophoma typhicola	MFLUCC 16-0123^T	KX576655	KX576656	–
Pseudodictyosporium elegans	CBS 688.93^T	DQ018099	DQ018106	–
Pseudodictyosporium indicum	CBS 471.95	DQ018097	–	–
Pseudodictyosporium thailandica	MFLUCC 16-0029^T	KX259520	KX259522	KX259526
Pseudodictyosporium wauense	NBRC 30078	DQ018098	DQ018105	–
Pseudodictyosporium wauense	DLUCC 0801	MF948622	MF948630	MF953165
Vikalpa australiensis	HKUCC 8797^T	DQ018092	–	–

Phylogenetic results

The analysed dataset consisted of combined ITS (557 bp), LSU (803 bp) and TEF1α (918 bp) sequence data (a total of 2278 characters including gaps) for 59 taxa in Dictyosporiaceae with Periconia igniaria E.W. Mason & M.B. Ellis (CBS 379.86, CBS 845.96) as the outgroup taxon. The best scoring RAxML tree is shown in Figure 1.

Figure 1.

Maximum likelihood majority rule consensus tree for the analysed Dictyosporiaceae isolates based on a dataset of combined ITS, LSU and TEF1α sequence data. Bootstrap support values for maximum likelihood (ML) and maximum parsimony (MP) greater than 75% and Bayesian posterior probabilities greater than 0.95 are indicated above the nodes as MLBS/MPBS/PP. The scale bar represents the expected number of changes per site. The tree is rooted with Periconia igniaria (CBS 379.86, CBS 845.96). The strain numbers are noted after the species names with ex-type strains indicated with ^T. The new collections are in bold with new taxa in red. Branches with 100% ML BS, 100% MP BS and 1.0 PP are shown as black nodes. Genera are indicated as coloured blocks.

Phylogenetic analyses indicated the placement of three isolates (MFLUCC 15-0056, MFLLUCC 16-0874 and MFLUCC 17-0222) within the genus Dictyocheirospora. Five isolates (MFLUCC 15-0629, MFLUCC 17-2052, MFLUCC 17-2056, MFLUCC 15-0631 and MFLUCC 17-2053) nested in Dictyosporium. Phylogenetic results showed that Dictyocheirospora indica (MFLUCC 15-0056) clustered with Dictyocheirospora subramanianii (B. Sutton) D'souza, Boonmee & K.D. Hyde (BCC 3503) with good support. Dictyocheirospora bannica (MFLUCC 16-0874) was placed as sister taxon to the ex-type strain Dictyocheirospora bannica (KH 332). Dictyocheirospora rotunda (MFLUCC 17-0222) grouped together with Dictyocheirospora rotunda (MFLUCC 17-1313) and the ex-type strain Dictyocheirospora rotunda (MFLUCC 14-0293) with strong support. The strain Dictyosporium sp. (MFLUCC 15-0629) clustered as sister taxon to Dictyosporium digitatum J.L. Chen, C.H. Hwang & Tzean (KH 401), Dictyosporium aquaticum Abdel-Aziz (MF 1318) and Dictyosporium stellatum G.P. White & Seifert (CCFC 241241). The new taxon Dictyosporium tratense (MFLUCC 17-2052) formed a single clade within Dictyosporium which is distinct from other species in the genus. The new collection Dictyosporium nigroapice (MFLUCC 17-2053) was placed as sister taxon to a previous isolate Dictyosporium nigroapice (BCC 3555). Two isolates of the new taxon Dictyosporium tubulatum (MFLUCC 15-0631 and MFLUCC 17-2056) nested in Dictyosporium as sister clade to Dictyosporium nigroapice (MFLUCC 17-2053 and BCC 3555).

Taxonomy

Dictyocheirospora species

Dictyocheirospora bannica Kaz. Tanaka, K. Hiray., Boonmee & K.D. Hyde, Fungal Diversity 80: 467 (2016)

Index Fungorum number: IF551997

Facesoffungi number: FoF02014

Figure 2

Material examined

THAILAND. Phang Nga Province, Bann Tom Thong Khang, on decaying wood submerged in a freshwater stream, 17 Dec 2015, J. Yang, Site 7-70-1 (MFLU 18-1040, HKAS 102131), living culture MFLUCC 16-0874 (Additional SSU sequence GenBank MH381759).

Notes

The phylogenetic result showed the strain MFLUCC 16-0874 clustered with the ex-type (KH 332) of Dictyocheirospora bannica. The morphological examination of this collection matched well with the holotype of Dictyocheirospora bannica (Boonmee et al. 2016). Dictyocheirospora bannica was previously collected in Japan, while this is a new record for Thailand.

Figure 2.

Dictyocheirospora bannica (MFLU 18-1040) a Colonies on submerged wood bConidia and conidiophores c–fConidiag Germinated conidium h, i Culture, h from above, i from reverse. Scale bars: a = 200 μm, b, g = 50 μm, c–f = 30 μm.

Dictyocheirospora hydei (I.B. Prasher & R.K. Verma) J. Yang & K.D. Hyde, comb. nov.

Index Fungorum number: IF554773

Facesoffungi number: FoF04679

Basionym

Dictyosporium hydei I.B. Prasher & R.K. Verma, Phytotaxa 204 (3): 196 (2015).

Holotype

INDIA. Himachal Pradesh, Bilaspur, on bark of Tecoma stans, 17 September 2013, I.B. Prasher and R.K. Verma (PAN 30364).

Notes

Considering the latest generic concept of Dictyocheirospora and Dictyosporium, we suggest that Dictyosporium hydei should be referred to Dictyocheirospora with the key character of non-complanate or cylindrical conidia with conidial arms closely gathered together at the apex. We have not examined the holotype of Dictyocheirospora hydei. The details provided by Prasher and Verma (2015) are adequate being illustrative and descriptive.

Dictyocheirospora indica (I.B. Prasher & R.K. Verma) J. Yang & K.D. Hyde, comb. nov.

Index Fungorum number: IF554774

Facesoffungi number: FoF04680

Figure 3

Basionym

Dictyosporium indicum I.B. Prasher & R.K. Verma, Phytotaxa 204 (3): 194 (2015).

Holotype

INDIA. Himachal Pradesh, Mandi, on petiole of Phoenix rupicola, 19 November 2012, I.B. Prasher and R.K. Verma (PAN 30313).

Material examined

THAILAND. Chiang Rai, stream flowing in Tham Luang Nang Non Cave, on decaying submerged wood, 25 November 2014, J. Yang, YJ-3 (MFLU 15-1169 reference specimen designated here, HKAS 102135), living culture MFLUCC 15-0056 (Additional SSU sequence GenBank MH381757).

Notes

Collection MFLU 15-1169 was identified as Dictyocheirospora indica (Dictyosporium indicum) based on morphological examination. Phylogenetic analyses indicated the placement of this taxon within Dictyocheirospora and sister to Di. subramanianii (BCC 3503). Dictyocheirospora subramanianii differs from Di. indica in lacking appendages. Dictyocheirospora indica resembles Di. musae in having non-complanate, cylindrical conidia with globose to subglobose appendages. However, conidial appendages of Di. indica are attached at the subapical cells, while appendages of Di. musae are attached at the central cells of the outer cell-row. The conidial size of Di. indica (33–48 × 13–18 µm) is smaller than that of Di. musae (45–65 × 20–27 µm) (Photita et al. 2002, Prasher and Verma 2015). In this study, sequence data of our collection Dictyocheirospora indica (MFLUCC 15-0056) was generated and, as there is no sequence data available for the previous collection (Dictyosporium indicum), we therefore designated our collection as the reference specimen (sensuAriyawansa et al. 2014) for Dictyocheirospora indica.

Figure 3.

Dictyocheirospora indica (MFLU 15-1169, reference specimen). a Substrate b, c Colonies on woody substrate d, e Conidial formation f–iConidia with partial conidiophores j–oConidiap Germinated conidium q–r Culture, q from above, r from reverse. Scale bars: b = 200 μm, c = 100 μm, d–i, l–o = 20 μm, j = 10 μm, k = 15 μm, p = 30 μm.

Dictyocheirospora musae (Photita) J. Yang, K.D. Hyde & Z.Y. Liu, comb. nov.

Index Fungorum number: IF554775

Facesoffungi number: FoF04681

Basionym

Dictyosporium musae Photita, Mycotaxon 82: 416 (2002)

Holotype

THAILAND. Mae Hong Son Province, Sob Mei, Huay Thicha Village, on decaying petioles of Musa acuminata, 23 November 2000, W. Photita (PDD 74135).

Notes. Dictyocheirospora musae is morphologically similar to Di. hydei in having non-complanate, cylindrical conidia with globose to subglobose appendages. However, Dictyocheirospora musae differs in having appendages in the middle cells while Di. hydei has appendages on the basal cells (Photita et al. 2002, Prasher and Verma 2015).

Dictyocheirospora rotunda D’souza, Bhat & K.D. Hyde, Fungal Diversity 80: 465 (2016)

Index Fungorum number: IF551581

Facesoffungi number: FoF01262

Figure 4

Material examined

CHINA. Guizhou Province, Anshun city, Gaodang village, 26°4.267'N, 105°41.883'E, on decaying wood submerged in Suoluo river, 19 October 2016, J. Yang, GD 2-3 (MFLU 18-1041, HKAS 102132), living culture MFLUCC 17-0222 (Additional SSU sequence GenBank MH381758).

Notes

This species is known in China and Thailand from freshwater habitats (Boonmee et al. 2016, Wang et al. 2016).

Figure 4.

Dictyocheirospora rotunda (MFLU 18-1041). a Colonies on submerged wood b, c Germinated conidia dConidiae, f Culture, e from above, f from reverse. Scale bars: a = 200 μm, b, c = 20 μm, d = 50 μm.

Dictyocheirospora tetraploides (L. Cai & K.D. Hyde) J. Yang & K.D. Hyde, comb. nov.

Index Fungorum number: IF554776

Facesoffungi number: FoF04682

Basionym

Dictyosporium tetraploides L. Cai & K.D. Hyde, Sydowia 55 (2): 132 (2003)

Holotype

CHINA. Yunnan, Xishuangbanna, Menglun, a small stream, on submerged wood, 21 June 2002, L. Cai (HKUM 17146).

Notes

Dictyocheirospora tetraploides is morphologically similar to Di. musae in conidial shape, size, colour and appendages. However, conidia of Di. tetraploides have 5-rowed cells, while those of Di. musae are 7-rowed cells (Photita et al. 2002, Cai et al. 2003).

Dictyosporium species

Dictyosporium tubulatum J. Yang, K.D. Hyde & Z.Y. Liu, sp. nov.

Index Fungorum number: IF554771

Facesoffungi number: FoF04677

Figure 5

Etymology

Referring to the tubular conidial appendages.

Description

Saprobic on decaying plant substrates. Asexual morph: Colonies punctiform, sporodochial, scattered, dark brown to black, glistening. Mycelium mostly immersed, composed of smooth, septate, branched, hyaline to pale brown hyphae. Conidiophores micronematous, mononematous, septate, cylindrical, hyaline to pale brown, smooth-walled, 6.5–15 × 3.5–6 μm, sometimes reduced to conidiogenous cells. Conidiogenous cells monoblastic, integrated, terminal, determinate, hyaline to pale brown. Conidia acrogenous, solitary, cheiroid, smooth-walled, complanate, yellowish-brown to medium brown, mostly consisting of four arms closely compact with side arms lower than middle arms, rarely with five arms, 5–7-euseptate in each arm, guttulate, (22–)29–35(–38) × (14–)17–19(–22) μm (x¯ = 32.5 × 18 μm, n = 40), with hyaline, tubular, elongated appendages which are 19–24 × 3.5–7 μm and mostly attached at the apical part of two outer arms. Sexual morph: Undetermined.

Cultural characteristics

Conidia germinating on PDA within 24 h and germ tubes produced from the basal cell. Colonies on MEA reaching 5–10 mm diam. in a week at 25 °C, in natural light, circular, with fluffy, dense, white mycelium on the surface with entire margin; in reverse yellow in the middle and white at the margin.

Material examined

THAILAND. Prachuap Khiri Khan Province, near 12°30.19'N, 99°31.35'E, on decaying wood submerged in a freshwater stream, 25 December 2014, J. van Strien, Site 5-11-1 (MFLU 15-1166 holotype, HKAS 102136 isotype), ex-type living culture MFLUCC 15-0631; ibid. Trat Province, Amphoe Ko Chang, 12°08'N, 102°38'E, on decaying wood submerged in a freshwater stream, 27 April 2017, Y.Z. Lu, YJT 22-2 (MFLU 18-1044, HKAS 102137 paratype), living culture MFLUCC 17-2056.

Notes

Phylogenetic analyses showed that Dictyosporium tubulatum nested in Dictyosporium and sister to D. nigroapice. Dictyosporium tubulatum morphologically resembles D. alatum Emden, D. canisporum L. Cai & K.D. Hyde and D. thailandicum D’ souza, D.J. Bhat & K.D. Hyde in conidial ontogeny and conidial shape, colour and appendages. Dictyosporium tubulatum differs from the three species in the number of conidial cell rows. There are mostly four conidial columns in D. tubulatum while mostly five columns in the others. Dictyosporium tubulatum has smaller conidia (25–38 × 14–22 μm) than those in D. canisporum (32.5–47.5 × 20–25 μm) but has similar conidial size with D. alatum (26–32 × 15–24 μm) and D. thailandicum (15.4–34.5 × 14.5–20.6 μm) (Cai et al. 2003, Liu et al. 2015). Based on the molecular phylogeny, D. tubulatum is distinct from D. thailandicum and D. alatum. Unfortunately, molecular data are unavailable for D. canisporum.

Figure 5.

Dictyosporium tubulatum (MFLU 15-1166, holotype). a, b Colonies on woody substrate c Squash mount of a sporodochium d–gConidiah–iConidia with conidiophores j–lConidia with appendages m lateral view of a conidium n Germinated conidium o, p Culture, o from above p from reverse. Scale bars: a = 1000 μm, b = 200 μm, c, n = 30 μm, d, e = 10 μm, f–m = 15 μm.

Dictyosporium tratense J. Yang & K.D. Hyde, sp. nov.

Index Fungorum number: IF554772

Facesoffungi number: FoF04678

Figure 6

Etymology

Referring to the collecting site in Trat province, Thailand.

Description

Saprobic> on decaying plant substrates. Asexual morph: Colonies punctiform, sporodochial, scattered, black, glistening. Mycelium mostly immersed, composed of smooth, septate, branched, hyaline to pale brown hyphae. Conidiophores micronematous, mononematous, septate, cylindrical, hyaline to pale brown, smooth-walled, sometimes reduced to conidiogenous cells. Conidiogenous cells monoblastic, integrated, terminal, determinate, hyaline to pale brown. Conidia (40–)43–54(–57) × (20–)23–32(–36) μm (x¯ = 49.5 × 26 μm, n = 40), acrogenous, solitary, cheiroid, smooth-walled, complanate, yellowish-brown to light brown, consisting of 39–68 cells arranged in 4–6 (mostly 5) closely compact columns, 9–11-euseptate in each column, guttulate; the inner columns nested within the outer columns, the outer columns derived from the basal cell of the conidium; the intermediate columns are derived from the first or second cell of the outer columns; the inner columns derived from the first or second cell of the intermediate columns; usually with 2–3 central columns longest and of equal length, 2–3 peripheral columns shorter and of equal length; sometimes with hyaline globose appendages at the apical cells of outer columns with hyaline cloud-shaped mucilaginous sheath. Sexual morph: Undetermined.

Cultural characteristics

Conidia germinating on PDA within 24 h and germ tubes produced from basal cell. Colonies on MEA reaching 5–10 mm diam. in a week at 25 °C, in natural light, circular, with fluffy, dense, pale yellow mycelium in the middle and sparse mycelium in the outer ring on the surface with irregular margin; in reverse, dark yellow to brown in the middle and pale yellow at the margin.

Material examined

THAILAND. Trat Province, Amphoe Ko Chang, 12°08'N, 102°38'E, on decaying wood submerged in a freshwater stream, 27 April 2017, Y.Z. Lu, YJT 6-2 (MFLU 18-1042 holotype, HKAS 102133 isotype), ex-type living culture MFLUCC 17-2052 (Additional SSU sequence GenBank MH381761).

Notes

Phylogenetic analyses indicated Dictyosporium tratense nested within Dictyosporium and close to D. wuyiense. It is distinguished from the other species in the genus in having a mucilaginous sheath. Morphologically, D. tratense is most comparable to D. elegans in conidial colour and shape, but conidia of the new taxon (40-57 × 20-36 μm) are smaller than those of D. elegans (40-80 × 24-36 μm) (Goh et al. 1999).

Figure 6.

Dictyosporium tratense (MFLU 18-1042, holotype). a Colonies on submerged wood b Squash mount of a sporodochium c Germinated conidium d–iConidiaj, k Culture j from above k from reverse. Scale bars: a = 200 μm, b = 50 μm, c = 30 μm, d–i = 20 μm.

Dictyosporium sp.

Figure 7

Material examined

THAILAND. Prachuap Khiri Khan Province, near 12°30.19'N, 99°31.35'E, on decaying wood submerged in a freshwater stream, 25 December 2014, J. van Strien, Site 5-5-1 (MFLU 15-1164), living culture MFLUCC 15-0629 (Additional SSU sequence GenBank MH381760).

Notes

Phylogenetic analyses indicated the isolate Dictyosporium sp. (MFLUCC 15-0629) was placed as sister taxon to D. digitatum (KH 401), D. aquaticum (MF 1318) and D. stellatum (CCFC 241241) with good support. The strain D. digitatum (KH 401), D. aquaticum (MF 1318) and our strain MFLUCC 15-0629, showed the same nucleotide (490 bp) between them for ITS gene regions, while there is only one nucleotide difference between our strain and D. stellatum (CCFC 241241). However, the strain Dictyosporium sp. (MFLUCC 15-0629) showed seven nucleotides different from D. digitatum (yone 280) for ITS gene regions. Morphologically, D. digitatum and D. aquaticum share the character in having appendages borne at the terminal cells of each conidial arm (Chen et al. 1991, Liu et al. 2015). Dictyosporium stellatum differs from D. digitatum and D. aquaticum in lacking conidial appendages (Crous et al. 2011). In this case, it is difficult to identify our collection based on the recommendations advocated by Jeewon and Hyde (2016) for differentiating species or establishing new species. Thus, we recommend designating this collection as unknown species until enough evidence is available for its identification.

Figure 7.

Dictyosporium sp. (MFLU 15-1164). a Colonies on submerged wood b Squash mount of a sporodochium; c Germinated conidium b–e, h, iConidiaf Germinated conidium gConidia with conidiophores j, k Culture, j from above, k from reverse. Scale bars: a = 200 μm, b, f–i = 30 μm, c = 50 μm d, e = 20 μm.

Dictyosporium nigroapice Goh, W.H. Ho & K.D. Hyde, Fungal Diversity 2: 83 (1999)

Index Fungorum number: IF450470

Facesoffungi number: FoF04683

Figure 8

Material examined

THAILAND. Trat Province, Amphoe Ko Chang, 12°08'N, 102°38'E, on decaying wood submerged in a freshwater stream, 27 April 2017, Y.Z. Lu, YJT 7-1 (MFLU 18-1043, HKAS 102134), living culture MFLUCC 17-2053 (Additional SSU sequence GenBank MH381762).

Notes

Conidia in Dictyosporium nigroapice are characterised by conspicuously darker apical cells of the two inner arms, rarely darker at the apex of the outer arms. Morphological characters of this collection well agree with the original diagnosis of the holotype of D. nigroapice (Goh et al. 1999).

Figure 8.

Dictyosporium nigroapice (MFLU18-1043). a Colonies on submerged wood b, cConidia and conidiophores d–jConidiak Germinated conidium l, m Culture, l from above, m from reverse. Scale bars: a = 100 μm, b, c, j = 20 μm, d–i = 10 μm, k = 30 μm.

Discussion

Dictyosporiaceae accommodates a holomorphic group of Dothideomycetes, including 12 genera with nine being dictyosporous (Wijayawardene et al. 2017b, Wijayawardene et al. 2018). Dictyocheirospora and Dictyosporium are the two largest genera in the family. Dictyosporium has cheiroid, digitate and complanate conidia without separating arms, while Dictyocheirospora is characterised by non-complanate conidia with arms arising from the basal cell and closely gathered at the apex and compact. Thus, Dictyosporium hydei, D. indicum, D. musae and D. tetraploides are transferred to Dictyocheirospora based on the clear morphological characters. Phylogenetic analyses revealed the placement of Dictyocheirospora indica (MFLUCC 15-0056 reference specimen) within Dictyocheirospora. We believe that the other three species belong to Dictyocheirospora in having similar conidia and appendages to Dictyocheirospora indica, although molecular data are unavailable for them.

Acknowledgement

We would like to thank The Research of Featured Microbial Resources and Diversity Investigation in the Southwest Karst area (project no. 2014FY120100) for its financial support. Kevin D. Hyde would like to thank the Thailand Research grants entitled “The future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants, Rhododendron species and Dracaena species” (grant no: DBG6080013) and “Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion” (grant no: RDG6130001) for supporting this study. Jing Yang thanks Shaun Pennycook for the corrections to the Latin names. Zong-Long Luo is acknowledged for the help with phylogenetic analyses.

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