Latest Articles from MycoKeys Latest 28 Articles from MycoKeys https://mycokeys.pensoft.net/ Fri, 29 Mar 2024 09:04:28 +0200 Pensoft FeedCreator https://mycokeys.pensoft.net/i/logo.jpg Latest Articles from MycoKeys https://mycokeys.pensoft.net/ Corrigendum: Hu H et al. (2023) Taxonomic and phylogenetic characterisations of six species of Pleosporales (in Didymosphaeriaceae, Roussoellaceae and Nigrogranaceae) from China. MycoKeys 100: 123–151. https://doi.org/10.3897/mycokeys.100.109423 https://mycokeys.pensoft.net/article/116896/ MycoKeys 102: 317-322

DOI: 10.3897/mycokeys.102.116896

Authors: Hongmin Hu, Minghui He, Youpeng Wu, Sihan Long, Xu Zhang, Lili Liu, Xiangchun Shen, Nalin N. Wijayawardene, Zebin Meng, Qingde Long, Jichuan Kang, Qirui Li

Abstract: Four new species, Xynobius azonius sp. nov., X. brevifemora sp. nov., X. duoferus sp. nov., and X. stipitoides sp. nov., are described and illustrated, and one species X. geniculatus (Thomson, 1895) is newly reported from South Korea. Xynobius geniculatus (Thomson, 1895) is redescribed and illustrated, and a new combination, Xynobius (Stigmatopoea) cubitalis (Fischer, 1959), comb. nov. is suggested. An identification key to the Xynobius species known from South Korea is provided.

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Corrigenda Thu, 7 Mar 2024 18:54:40 +0200
Taxonomic novelties and global biogeography of Montagnula (Ascomycota, Didymosphaeriaceae) https://mycokeys.pensoft.net/article/113259/ MycoKeys 101: 191-232

DOI: 10.3897/mycokeys.101.113259

Authors: Dhanushka N. Wanasinghe, Thilina S. Nimalrathna, Li Qin Xian, Turki Kh. Faraj, Jianchu Xu, Peter E. Mortimer

Abstract: Whilst conducting surveys of lignicolous microfungi in Yunnan Province, we collected a large number of taxa that resemble Montagnula (Didymosphaeriaceae, Pleosporales). Our phylogenetic study on Montagnula involved analysing sequence data from ribosomal RNA genes (nc18S, nc28S, ITS) and protein-coding genes (rpb2, tef1-α). We present a biphasic approach (morphological and molecular phylogenetic evidence) that supports the recognition of four new species in Montagnula viz., M. lijiangensis, M. menglaensis, M. shangrilana and M. thevetiae. The global diversity of Montagnula is also inferred from metabarcoding data and published records based on field observations. Metabarcoding data from GlobalFungi and field observations provided insights into the global diversity and distribution patterns of Montagnula. Studies conducted in Asia, Australia, Europe, and North America revealed a concentration of Montagnula species, suggesting regional variations in ecological preferences and distribution. Montagnula species were found on various substrates, with sediments yielding a high number of sequences. Poaceae emerged as a significant contributor, indicating a potential association between Montagnula species and grasses. Culture-based investigations from previously published data revealed Montagnula species associations with 105 plant genera (in 45 plant families), across 55 countries, highlighting their wide ecological range and adaptability. This study enhances our understanding of the taxonomy, distribution, and ecological preferences of Montagnula species. It emphasizes their role in the decomposition of organic matter in grasslands and savannah systems and suggests further investigation into their functional roles in ecosystem processes. The global distribution patterns and ecological interactions of Montagnula species underscore the need for continued research and conservation efforts.

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Research Article Fri, 19 Jan 2024 10:12:55 +0200
Morphological characteristics and phylogenetic evidence reveal two new species and the first report of Comoclathris (Pleosporaceae, Pleosporales) on dicotyledonous plants from China https://mycokeys.pensoft.net/article/113040/ MycoKeys 101: 95-112

DOI: 10.3897/mycokeys.101.113040

Authors: Rong Xu, Wenxin Su, Yang Wang, Shangqing Tian, Yu Li, Chayanard Phukhamsakda

Abstract: Two novel Comoclathris species were identified from dicotyledonous plants (Clematis sp. and Xanthoceras sorbifolium) in China. The results were supported by morphological characters and Maximum Likelihood (ML) and Bayesian Inference (BI) analyses. Multi-gene phylogenetic analyses of the ITS, LSU, SSU and rpb2 sequences revealed two new species Comoclathris clematidis and C. xanthoceratis, which are phylogenetically distinct. The new species are phylogenetically closely related to C. arrhenatheri. However, they are distinguishable from C. arrhenatheri by having comparatively larger asci and ascospores. This study improves our knowledge of Comoclathris as no species has been previously described from China. This suggests such taxa may be rare and it is likely that new taxa will be discovered from hosts and environments that have not yet been extensively investigated.

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Research Article Fri, 12 Jan 2024 11:20:08 +0200
Seven new species of Alternaria (Pleosporales, Pleosporaceae) associated with Chinese fir, based on morphological and molecular evidence https://mycokeys.pensoft.net/article/115370/ MycoKeys 101: 1-44

DOI: 10.3897/mycokeys.101.115370

Authors: Jiao He, De-Wei Li, Wen-Li Cui, Lin Huang

Abstract: Chinese fir (Cunninghamia lanceolata) is a special fast-growing commercial tree species in China and has significant ecological and economic value. However, it experienced damage from leaf blight caused by pathogenic fungi of the genus Alternaria. To determine the diversity of Alternaria species associated with leaf blight of Chinese fir in China, infected leaves were collected from five major cultivation provinces (Fujian, Henan, Hunan, Jiangsu and Shandong provinces). A total of 48 fungal strains of Alternaria were obtained. Comparison of morphology and phylogenetic analyses, based on nine loci (ITS, SSU, LSU, GAPDH, RPB2, TEF1, Alt a1, endoPG and OPA10-2) of the representative isolates as well as the pairwise homoplasy index tests, revealed that the fungal strains belonged to seven undescribed taxa of Alternaria, which are described here and named as Alternaria cunninghamiicola sp. nov., A. dongshanqiaoensis sp. nov., A. hunanensis sp. nov., A. kunyuensis sp. nov., А. longqiaoensis sp. nov., A. shandongensis sp. nov. and A. xinyangensis sp. nov. In order to prove Koch’s postulates, pathogenicity tests on detached Chinese fir leaves revealed significant pathogenicity amongst these species, of which A. hunanensis is the most pathogenic to Chinese fir. This study represents the first report of A. cunninghamiicola, A. dongshanqiaoensis, A. hunanensis, A. kunyuensis, A. longqiaoensis, A. shandongensis and A. xinyangensis causing leaf blight on Chinese fir. Knowledge obtained in this study enhanced our understanding of Alternaria species causing leaf blight on Chinese fir and was crucial for the disease management and the further studies in the future.

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Research Article Fri, 5 Jan 2024 14:17:42 +0200
Morphophylogenetic evidence reveals four new fungal species within Tetraplosphaeriaceae (Pleosporales, Ascomycota) from tropical and subtropical forest in China https://mycokeys.pensoft.net/article/113141/ MycoKeys 100: 171-204

DOI: 10.3897/mycokeys.100.113141

Authors: Xia Tang, Rajesh Jeewon, Yong-Zhong Lu, Abdulwahed Fahad Alrefaei, Ruvishika S. Jayawardena, Rong-Ju Xu, Jian Ma, Xue-Mei Chen, Ji-Chuan Kang

Abstract: Tetraplosphaeriaceae (Pleosporales, Ascomycota) is a family with many saprobes recorded from various hosts, especially bamboo and grasses. During a taxonomic investigation of microfungi in tropical and subtropical forest regions of Guizhou, Hainan and Yunnan provinces, China, several plant samples were collected and examined for fungi. Four newly discovered species are described based on morphology and evolutionary relationships with their allies inferred from phylogenetic analyses derived from a combined dataset of LSU, ITS, SSU, and tub2 DNA sequence data. Detailed illustrations, descriptions and taxonomic notes are provided for each species. The four new species of Tetraplosphaeriaceae reported herein are Polyplosphaeria guizhouensis, Polyplosphaeria hainanensis, Pseudotetraploa yunnanensis, and Tetraploa hainanensis. A checklist of Tetraplosphaeriaceae species with available details on their ecology is also provided.

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Research Article Wed, 6 Dec 2023 14:40:37 +0200
Taxonomic and phylogenetic characterisations of six species of Pleosporales (in Didymosphaeriaceae, Roussoellaceae and Nigrogranaceae) from China https://mycokeys.pensoft.net/article/109423/ MycoKeys 100: 123-151

DOI: 10.3897/mycokeys.100.109423

Authors: Hongmin Hu, Minghui He, Youpeng Wu, Sihan Long, Xu Zhang, Lili Liu, Xiangchun Shen, Nalin N. Wijayawardene, Zebin Meng, Qingde Long, Jichuan Kang, Qirui Li

Abstract: Pleosporales comprise a diverse group of fungi with a global distribution and significant ecological importance. A survey on Pleosporales (in Didymosphaeriaceae, Roussoellaceae and Nigrogranaceae) in Guizhou Province, China, was conducted. Specimens were identified, based on morphological characteristics and phylogenetic analyses using a dataset composed of ITS, LSU, SSU, tef1 and rpb2 loci. Maximum Likelihood (ML) and Bayesian analyses were performed. As a result, three new species (Neokalmusia karka, Nigrograna schinifolium and N. trachycarpus) have been discovered, along with two new records for China (Roussoella neopustulans and R. doimaesalongensis) and a known species (Roussoella pseudohysterioides). Morphologically similar species and phylogenetically close taxa are compared and discussed. This study provides detailed information and descriptions of all newly-identified taxa.

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Research Article Wed, 29 Nov 2023 18:10:36 +0200
Morphological and phylogenetic analyses reveal three new species and one new record of Tubeufia (Tubeufiales, Tubeufiaceae) from southern China https://mycokeys.pensoft.net/article/107606/ MycoKeys 99: 87-108

DOI: 10.3897/mycokeys.99.107606

Authors: Jian Ma, Li-Juan Zhang, Saranyaphat Boonmee, Xing-Juan Xiao, Ning-Guo Liu, Yuan-Pin Xiao, Zong-Long Luo, Yong-Zhong Lu

Abstract: During an investigation of helicosporous fungi in China, a total of seven helicosporous hyphomycetes were obtained from decaying wood in the southern region of the country. Based on phylogenetic analyses using a combined LSU, ITS, tef1α, and rpb2 sequence matrix, in conjunction with morphological comparisons, these taxa were classified within Tubeufia (Tubeufiaceae, Tubeufiales) and were recognized as three new species, viz. Tubeufia guttulata, T. hainanensis, and T. muriformis, as well as one new distribution record, viz. T. cocois. Evidence for these new taxa and the new record, descriptions, illustrations, notes, and phylogenetic evidence are provided for the newly collected helicosporous species.

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Research Article Mon, 14 Aug 2023 17:50:32 +0300
Two novel species and three new records of Torulaceae from Yunnan Province, China https://mycokeys.pensoft.net/article/106699/ MycoKeys 99: 1-24

DOI: 10.3897/mycokeys.99.106699

Authors: Wen-Peng Wang, Hong-Wei Shen, Dan-Feng Bao, Yong-Zhong Lu, Qiu-Xia Yang, Xi-Jun Su, Zong-Long Luo

Abstract: While investigating the diversity of lignicolous fungi in Yunnan Province, China, six fresh collections of Torulaceae were collected and identified based on morphological examination and phylogenetic analyses of combined LSU, ITS, SSU, tef1-α, and rpb2 sequence data. Two new species, viz. Neopodoconis yunnanensis and Torula suae, and three new records, viz. T. canangae (new freshwater habitat record), T. masonii (new host record), and T. sundara (new freshwater habitat record) are reported. Detailed descriptions, illustrations, and a phylogenetic tree to show the placement of these species are provided.

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Research Article Mon, 7 Aug 2023 15:55:26 +0300
Pleomorphic Dematiomelanomma yunnanense gen. et sp. nov. (Ascomycota, Melanommataceae) from grassland vegetation in Yunnan, China https://mycokeys.pensoft.net/article/107093/ MycoKeys 98: 273-297

DOI: 10.3897/mycokeys.98.107093

Authors: Ying Gao, Tingfang Zhong, Jayarama D. Bhat, Antonio Roberto Gomes de Farias, Turki M. Dawoud, Kevin D. Hyde, Weiqiang Xiong, Yunju Li, Heng Gui, Xuefei Yang, Shixi Wu, Dhanushka N. Wanasinghe

Abstract: During a survey of microfungi associated with grasslands and related vegetation types from Yunnan Province in China, various ascomycetous and coelomycetous fungi were isolated. This study reports the discovery of four strains of ascomycetous and coelomycetous fungi from dead stalks of Hypericum monogynum L. (Hypericaceae) and Rubus parvifolius L. (Rosaceae) in the Zhaotong region of Yunnan Province, China. The isolates were characterized using multi-locus phylogenetic analyses and were found to represent a new monophyletic lineage in Melanommataceae (Pleosporales, Dothideomycetes). This new clade was named as Dematiomelanomma yunnanense gen. et sp. nov. which consists of both sexual and asexual morphs. The sexual morph is characterized by globose to subglobose ascomata with a central ostiole, cylindrical asci with a pedicel and ocular chamber, and muriform, ellipsoidal to fusiform ascospores. The asexual morph has synanamorphs including both brown, muriform macroconidia and hyaline, round to oblong or ellipsoidal microconidia. These findings contribute to the understanding of fungal diversity in grasslands and related vegetation types in Yunnan Province, China.

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Research Article Tue, 25 Jul 2023 11:57:13 +0300
Three novel species of Helminthosporium (Massarinaceae, Pleosporales) from China https://mycokeys.pensoft.net/article/95888/ MycoKeys 94: 73-89

DOI: 10.3897/mycokeys.94.95888

Authors: Jingwen Liu, Yafen Hu, Xingxing Luo, Rafael F. Castañeda-Ruíz, Jian Ma

Abstract: Three new species of Helminthosporium, H. nabanhensis, H. sinensis and H. yunnanensis collected on dead branches of unidentified plants in Xishuangbanna, China, were proposed by morphological and molecular phylogenetic analysis. Phylogenetic analysis of the combined data of ITS-SSU-LSU-TEF1-RPB2 sequences was performed using Maximum-Likelihood and Bayesian Inference, although H. nabanhensis and H. sinensis lack the RPB2 sequences. Both molecular analyses and morphological data supported H. nabanhensis, H. sinensis and H. yunnanensis as three independent taxa within the Massarinaceae.

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Research Article Wed, 21 Dec 2022 10:30:30 +0200
Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China https://mycokeys.pensoft.net/article/95751/ MycoKeys 94: 51-71

DOI: 10.3897/mycokeys.94.95751

Authors: Li Lu, Samantha C. Karunarathna, Dong-qin Dai, Ruvishika S. Jayawardena, Nakarin Suwannarach, Saowaluck Tibpromma

Abstract: Coffee is one of the most important cash crops in Yunnan Province, China. Yunnan is ranked as the biggest producer of high-quality coffee in China. During surveys of microfungi from coffee plantations in Yunnan, six fungal strains that resemble Nigrogranaceae were collected. Multi-gene analyses of a combined SSU-LSU-ITS-rpb2-tef1-α sequence data matrix were used to infer the phylogenetic position of the new species in Nigrograna while morphological characteristics were used to deduce the taxonomic position of the new species. Six fungal strains isolated from decaying branches of Coffea arabica represent three new saprobic species in Nigrograna. The three new species, N. asexualis, N. coffeae, and N. puerensis, are described with full (macro and micro characteristics) descriptions, illustrations, and a phylogenetic tree that shows the phylogenetic position of new taxa.

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Research Article Mon, 12 Dec 2022 10:40:56 +0200
Pleocatenata chiangraiensis gen. et. sp. nov. (Pleosporales, Dothideomycetes) from medicinal plants in northern Thailand https://mycokeys.pensoft.net/article/79433/ MycoKeys 87: 77-98

DOI: 10.3897/mycokeys.87.79433

Authors: Ya-Ru Sun, Ning-Guo Liu, Kevin D. Hyde, Ruvishika S. Jayawardena, Yong Wang

Abstract: Pleocatenata, a new genus, is introduced with its type species, Pleocatenata chiangraiensis, which was isolated from withered twigs of two medicinal plants, Clerodendrum quadriloculare (Blanco) Merr (Verbenaceae) and Tarenna stellulata (Hook.f.) Ridl (Rubiaceae) in northern Thailand. The genus is characterized by mononematous, septate, brown or dark brown conidiophores, monotretic conidiogenous cells and catenate, obclavate, olivaceous to blackish brown conidia. Phylogenetic analysis of combined LSU, SSU, tef1-α, rpb2 and ITS sequence data showed Pleocatenata forms a distinct phylogenetic lineage in Pleosporales, Dothideomycetes. Therefore, we treat Pleocatenata as Pleosporales genera incertae sedis based on morphology and phylogenetic analyses. Descriptions and illustrations of the new taxa are provided, and it is compared with morphologically similar genera.

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Research Article Fri, 11 Feb 2022 10:19:34 +0200
Novel saprobic Hermatomyces species (Hermatomycetaceae, Pleosporales) from China (Yunnan Province) and Thailand https://mycokeys.pensoft.net/article/67973/ MycoKeys 82: 57-79

DOI: 10.3897/mycokeys.82.67973

Authors: Guang-Cong Ren, Dhanushka N. Wanasinghe, Jutamart Monkai, Peter E. Mortimer, Kevin D. Hyde, Jian-Chu Xu, Aimin Pang, Heng Gui

Abstract: During our survey of the diversity of woody litter fungi in China and Thailand, three Hermatomyces species were collected from dead woody twigs of Dipterocarpus sp. (Dipterocarpaceae) and Ehretia acuminata (Boraginaceae). Both morphology and multigene analyses revealed two taxa as new species (Hermatomyces turbinatus and H. jinghaensis) and the remaining collections as new records of H. sphaericus. Hermatomyces turbinatus is characterized by 1) dimorphic conidia, having circular to oval lenticular conidia and 2) turbinate conidia consisting of two columns with two septa composed of 2–3 cells in each column. Hermatomyces jinghaensis is characterized by dimorphic conidia, having circular to oval lenticular conidia and clavate or subcylindrical to cylindrical conidia and consisting of one or two columns with 6–8 cells in each column. Phylogenetic analyses of combined LSU, ITS, tub2, tef1-α and rpb2 sequence data supports the placement of these new taxa within Hermatomycetaceae with high statistical support.

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Research Article Mon, 9 Aug 2021 15:36:43 +0300
Morphological and molecular identification of two new Alternaria species (Ascomycota, Pleosporaceae) in section Radicina from China https://mycokeys.pensoft.net/article/64853/ MycoKeys 78: 187-198

DOI: 10.3897/mycokeys.78.64853

Authors: Lin He, Hong Cheng, Lin Zhao, Aye Aye Htun, Zhi He Yu, Jian Xin Deng, Qi Li Li

Abstract: The fungal genus Alternaria was distributed widely and found in different habitats such as plant or indoor environment. During an investigation into this genus in China, two new Alternaria species, Alternaria vulgarae and A. divaricatae were respectively isolated from diseased leaves of Foeniculum vulgare and Saposhnikovia divaricata, which both belonged to Umbelliferae. Phylogenetically, they were determined as new species belonging in the section Radicina of Alternaria based on the combined four gene fragments of ITS, TEF1, GAPDH and RPB2. Morphologically, the two species were illustrated and compared with other relevant Alternaria species in section Radicina.

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Research Article Fri, 9 Apr 2021 10:05:35 +0300
Cryptic diversity found in Didymellaceae from Australian native legumes https://mycokeys.pensoft.net/article/60063/ MycoKeys 78: 1-20

DOI: 10.3897/mycokeys.78.60063

Authors: Elizabeth C. Keirnan, Yu Pei Tan, Matthew H. Laurence, Allison A. Mertin, Edward C. Y. Liew, Brett A. Summerell, Roger G. Shivas

Abstract: Ascochyta koolunga (Didymellaceae, Pleosporales) was first described in 2009 (as Phoma koolunga) and identified as the causal agent of Ascochyta blight of Pisum sativum (field pea) in South Australia. Since then A. koolunga has not been reported anywhere else in the world, and its origins and occurrence on other legume (Fabaceae) species remains unknown. Blight and leaf spot diseases of Australian native, pasture and naturalised legumes were studied to investigate a possible native origin of A. koolunga. Ascochyta koolunga was not detected on native, naturalised or pasture legumes that had leaf spot symptoms, in any of the studied regions in southern Australia, and only one isolate was recovered from P. sativum. However, we isolated five novel species in the Didymellaceae from leaf spots of Australian native legumes from commercial field pea regions throughout southern Australia. The novel species were classified on the basis of morphology and phylogenetic analyses of the internal transcribed spacer region and part of the RNA polymerase II subunit B gene region. Three of these species, Nothophoma garlbiwalawarda sp. nov., Nothophoma naiawu sp. nov. and Nothophoma ngayawang sp. nov., were isolated from Senna artemisioides. The other species described here are Epicoccum djirangnandiri sp. nov. from Swainsona galegifolia and Neodidymelliopsis tinkyukuku sp. nov. from Hardenbergia violacea. In addition, we report three new host-pathogen associations in Australia, namely Didymella pinodes on S. artemisioides and Vicia cracca, and D. lethalis on Lathyrus tingitanus. This is also the first report of Didymella prosopidis in Australia.

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Research Article Mon, 8 Feb 2021 16:14:10 +0200
Multi-gene phylogenetic evidence suggests Dictyoarthrinium belongs in Didymosphaeriaceae (Pleosporales, Dothideomycetes) and Dictyoarthrinium musae sp. nov. on Musa from Thailand https://mycokeys.pensoft.net/article/55493/ MycoKeys 71: 101-118

DOI: 10.3897/mycokeys.71.55493

Authors: Binu C. Samarakoon, Dhanushka N. Wanasinghe, Milan C. Samarakoon, Rungtiwa Phookamsak, Eric H.C. McKenzie, Putarak Chomnunti, Kevin D. Hyde, Saisamorn Lumyong, Samantha C. Karunarathna

Abstract: Dead leaves of Musa sp. (banana) were collected in northern Thailand during an investigation of saprobic fungi. Preliminary morphological observations revealed that three specimens belong to Dictyoarthrinium. Phylogenetic analyses of combined SSU, LSU, ITS and tef1-α sequence data revealed that Dictyoarthrinium forms a clade in Didymosphaeriaceae (Massarineae, Pleosporales, Dothideomycetes) sister to Spegazzinia. Based on contrasting morphological features with the extant taxa of Dictyoarthrinium, coupled with the multigene analyses, Dictyoarthrinium musae sp. nov. is introduced herein. Our study provides the first detailed molecular investigation for Dictyoarthrinium and supports its placement in Didymosphaeriaceae (Massarineae, Pleosporales, Dothideomycetes). Previously, Dictyoarthrinium was classified in Apiosporaceae (Xylariales, Sordariomycetes).

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Research Article Wed, 5 Aug 2020 08:19:27 +0300
Additions to Phaeosphaeriaceae (Pleosporales): Elongaticollum gen. nov., Ophiosphaerella taiwanensis sp. nov., Phaeosphaeriopsis beaucarneae sp. nov. and a new host record of Neosetophoma poaceicola from Musaceae https://mycokeys.pensoft.net/article/53674/ MycoKeys 70: 59-88

DOI: 10.3897/mycokeys.70.53674

Authors: Danushka S. Tennakoon, Kasun M. Thambugala, Dhanushka N. Wanasinghe, Eleni Gentekaki, Itthayakorn Promputtha, Chang-Hsin Kuo, Kevin D. Hyde

Abstract: A novel ascomycetous genus, Elongaticollum, occurring on leaf litter of Hedychium coronarium (Zingiberaceae) in Taiwan, is described and illustrated. Elongaticollum is characterized by dark brown to black, superficial, obpyriform, pycnidial conidiomata with a distinct elongate neck, and oval to oblong, hyaline, aseptate conidia. Phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian) of combined ITS, LSU, SSU and tef1-α sequence data revealed Elongaticollum as a distinct genus within the family Phaeosphaeriaceae with high statistical support. In addition, Ophiosphaerella taiwanensis and Phaeosphaeriopsis beaucarneae are described as new species from dead leaves of Agave tequilana and Beaucarnea recurvata (Asparagaceae), respectively. Neosetophoma poaceicola is reported as a new host record from dead leaves of Musa acuminata (Musaceae). Newly described taxa are compared with other similar species and comprehensive descriptions and micrographs are provided.

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Research Article Mon, 27 Jul 2020 08:22:50 +0300
Taxonomy and phylogenetic appraisal of Spegazzinia musae sp. nov. and S. deightonii (Didymosphaeriaceae, Pleosporales) on Musaceae from Thailand https://mycokeys.pensoft.net/article/52043/ MycoKeys 70: 19-37

DOI: 10.3897/mycokeys.70.52043

Authors: Binu C. Samarakoon, Rungtiwa Phookamsak, Dhanushka N. Wanasinghe, Putarak Chomnunti, Kevin D. Hyde, Eric H. C. Mckenzie, Itthayakorn Promputtha, Jian-Chu Xu, Yun-Ju Li

Abstract: Tropical plants host a range of fungal niches including endophytes, pathogens, epiphytes and saprobes. A study undertaken to discover the saprobic fungal species associated with Musa sp. (banana) from northern Thailand found two hyphomycetous taxa of Spegazzinia (Didymosphaeriaceae, Pleosporales). These were collected during the dry season and their morpho-molecular taxonomic relationships were investigated. Based on phylogenetic analysis of combined SSU, LSU, ITS and TEF1-α sequence data (77% ML, 0.99 BYPP) and contrasting morphological features to the sister taxon, we introduce Spegazzinia musae as a novel species from a decaying leaf of Musa sp. Details on the taxonomy, ecology and geographical distribution of Spegazzinia species are provided. In addition, we report S. deightonii as a new host record from Musa sp. Our data further validate the taxonomic placement of Spegazzinia in Didymosphaeriaceae.

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Research Article Tue, 21 Jul 2020 17:01:04 +0300
Lignicolous freshwater ascomycota from Thailand: Phylogenetic and morphological characterisation of two new freshwater fungi: Tingoldiago hydei sp. nov. and T. clavata sp. nov. from Eastern Thailand https://mycokeys.pensoft.net/article/49769/ MycoKeys 65: 119-138

DOI: 10.3897/mycokeys.65.49769

Authors: Li Xu, Dan-Feng Bao, Zong-Long Luo, Xi-Jun Su, Hong-Wei Shen, Hong-Yan Su

Abstract: Lignicolous freshwater fungi represent one of the largest groups of Ascomycota. This taxonomically highly diverse group plays an important role in nutrient and carbon cycling, biological diversity and ecosystem functioning. The diversity of lignicolous freshwater fungi along a north-south latitudinal gradient is currently being studied in Asia. In this paper, we introduce two novel freshwater taxa viz. Tingoldiago hydei sp. nov. and T. clavata sp. nov. which were collected from freshwater substrates in Eastern Thailand. Morphological comparison based on the size of ascomata, asci and ascospores, as well as multi-gene phylogenetic analyses based on LSU, SSU, ITS and TEF1-α DNA sequences, supports their placement in Tingoldiago (Lentitheciaceae). Descriptions and illustrations of these two new species are provided.

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Research Article Thu, 26 Mar 2020 15:15:58 +0200
Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes) https://mycokeys.pensoft.net/article/47704/ MycoKeys 65: 49-99

DOI: 10.3897/mycokeys.65.47704

Authors: Lingwei Hou, Margarita Hernández-Restrepo, Johannes Zacharias Groenewald, Lei Cai, Pedro W. Crous

Abstract: Fungal communities play a crucial role in maintaining the health of managed and natural soil environments, which directly or indirectly affect the properties of plants and other soil inhabitants. As part of a Citizen Science Project initiated by the Westerdijk Fungal Biodiversity Institute and the Utrecht University Museum, which aimed to describe novel fungal species from Dutch garden soil, the diversity of Didymellaceae, which is one of the largest families in the Dothideomycetes was investigated. A preliminary analysis of the ITS and LSU sequences from the obtained isolates allowed the identification of 148 strains belonging to the family. Based on a multi-locus phylogeny of a combined ITS, LSU, rpb2 and tub2 alignment, and morphological characteristics, 20 different species were identified in nine genera, namely Ascochyta, Calophoma, Didymella, Juxtiphoma, Nothophoma, Paraboeremia, Phomatodes, Stagonosporopsis, and Xenodidymella. Several isolates confirmed to be ubiquitous plant pathogens or endophytes were for the first time identified from soil, such as Ascochyta syringae, Calophoma clematidis-rectae, and Paraboeremia litseae. Furthermore, one new genus and 12 novel species were described from soil: Ascochyta benningiorum sp. nov., Didymella degraaffiae sp. nov., D. kooimaniorum sp. nov., Juxtiphoma kolkmaniorum sp. nov., Nothophoma brennandiae sp. nov., Paraboeremia rekkeri sp. nov., P. truiniorum sp. nov., Stagonosporopsis stuijvenbergii sp. nov., S. weymaniae sp. nov., Vandijckomycella joseae gen. nov. et sp. nov., V. snoekiae sp. nov., and Xenodidymella weymaniae sp. nov. From the results of this study, soil was revealed to be a rich substrate for members of Didymellaceae, several of which were previously known only from diseased or apparently healthy plant hosts.

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Research Article Tue, 10 Mar 2020 11:18:56 +0200
Neptunomyces aureus gen. et sp. nov. (Didymosphaeriaceae, Pleosporales) isolated from algae in Ria de Aveiro, Portugal https://mycokeys.pensoft.net/article/37931/ MycoKeys 60: 31-44

DOI: 10.3897/mycokeys.60.37931

Authors: Micael F. M. Gonçalves, Tânia F. L. Vicente, Ana C. Esteves, Artur Alves

Abstract: A collection of fungi was isolated from macroalgae of the genera Gracilaria, Enteromorpha and Ulva in the estuary Ria de Aveiro in Portugal. These isolates were characterized through a multilocus phylogeny based on ITS region of the ribosomal DNA, beta-tubulin (tub2) and translation elongation factor 1 alpha (tef1-α) sequences, in conjunction with morphological and physiological data. These analyses showed that the isolates represented an unknown fungus for which a new genus, Neptunomyces gen. nov. and a new species, Neptunomyces aureus sp. nov. are proposed. Phylogenetic analyses supported the affiliation of this new taxon to the family Didymosphaeriaceae.

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Research Article Thu, 31 Oct 2019 13:41:45 +0200
Rubroshiraia gen. nov., a second hypocrellin-producing genus in Shiraiaceae (Pleosporales) https://mycokeys.pensoft.net/article/36723/ MycoKeys 58: 1-26

DOI: 10.3897/mycokeys.58.36723

Authors: Dong-Qin Dai, Nalin N. Wijayawardene, Li-Zhou Tang, Chao Liu, Li-Hong Han, Hong-Long Chu, Hai-Bo Wang, Chun-Fang Liao, Er-Fu Yang, Rui-Fang Xu, Yun-Min Li, Kevin D. Hyde, D. Jayarama Bhat, Paul F. Cannon

Abstract: Shiraiaceae is an important family in Pleosporales (Dothideomycetes), which includes medical fungi and plant pathogens. Two hypocrellin-producing taxa, Shiraia bambusicola and a novel genus Rubroshiraia gen. nov., typified by Rubroshiraia bambusae are treated in this article. Maximum likelihood analysis, generated via RAxML (GTR+G model), using a combined SSU, LSU, TEF1 and RPB2 sequence dataset, shows that Rubroshiraia is close to Shiraia and belongs to the family Shiraiaceae. Descriptions, illustrations and a taxonomic key are provided for the genera in Shiraiaceae. Rubroshiraia morphologically differs from Shiraia in having small and dark ascostromata and filiform ascospores. Production of the ascostromatal metabolites, hypocrellin A and B, were examined by HPLC and spectrophotometer. The content of hypocrellin A and B of specimen HKAS 102255 (R. bambusae) is twice that produced by HKAS 102253 (S. bambusicola). To clarify the relationship between R. bambusae and Hypocrella bambusae, type material of the latter was examined and provided the illustration.

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Research Article Wed, 28 Aug 2019 13:40:50 +0300
Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica https://mycokeys.pensoft.net/article/35682/ MycoKeys 55: 59-86

DOI: 10.3897/mycokeys.55.35682

Authors: Martin Vohník, Ondřej Borovec, Zuzana Kolaříková, Radka Sudová, Martina Réblová

Abstract: Seagrasses provide invaluable ecosystem services yet very little is known about their root mycobiont diversity and distribution. Here we focused on the dominant Mediterranean seagrass Posidonia oceanica and assessed its root mycobiome at 32 localities covering most of the ecoregions in the NW Mediterranean Sea using light and scanning electron microscopy and tag-encoded 454-pyrosequencing. Microscopy revealed that the recently discovered dark septate endophytic association specific for P. oceanica is present at all localities and pyrosequencing confirmed that the P. oceanica root mycobiome is dominated by a single undescribed pleosporalean fungus, hitherto unknown from other hosts and ecosystems. Its numerous slow-growing isolates were obtained from surface-sterilised root segments at one locality and after prolonged cultivation, several of them produced viable sterile mycelium. To infer their phylogenetic relationships we sequenced and analysed the large (LSU) and small (SSU) subunit nrDNA, the ITS nrDNA and the DNA-directed RNA polymerase II (RPB2). The fungus represents an independent marine biotrophic lineage in the Aigialaceae (Pleosporales) and is introduced here as Posidoniomyces atricolor gen. et sp. nov. Its closest relatives are typically plant-associated saprobes from marine, terrestrial and freshwater habitats in Southeast Asia and Central America. This study expands our knowledge and diversity of the Aigialaceae, adds a new symbiotic lifestyle to this family and provides a formal name for the dominant root mycobiont of the dominant Mediterranean seagrass.

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Research Article Wed, 26 Jun 2019 11:37:54 +0300
Striatiguttulaceae, a new pleosporalean family to accommodate Longicorpus and Striatiguttula gen. nov. from palms https://mycokeys.pensoft.net/article/30886/ MycoKeys 49: 99-129

DOI: 10.3897/mycokeys.49.30886

Authors: Sheng-Nan Zhang, Kevin D. Hyde, E.B. Gareth Jones, Rajesh Jeewon, Ratchadawan Cheewangkoon, Jian-Kui Liu

Abstract: Palms represent the most morphological diverse monocotyledonous plants and support a vast array of fungi. Recent examinations of palmicolous fungi in Thailand led to the discovery of a group of morphologically similar and interesting taxa. A polyphasic approach based on morphology, multi-gene phylogenetic analyses and divergence time estimates supports the establishment of a novel pleosporalean family Striatiguttulaceae, which diversified approximately 39 (20–63) MYA (crown age) and 60 (35–91) MYA (stem age). Striatiguttulaceae is characterized by stromata or ascomata with a short to long neck, trabeculate pseudoparaphyses and fusiform to ellipsoidal, 1–3-septate ascospores, with longitudinal striations and paler end cells, surrounded by a mucilaginous sheath. Multi-gene phylogenetic analysis showed that taxa of Striatiguttulaceae form a well-supported and distinct monophyletic clade in Pleosporales, and related to Ligninsphaeriaceae and Pseudoastrosphaeriellaceae. However, these families can be morphologically demarcated by the slit-like ascomata and extremely large ascospores in Ligninsphaeriaceae and the rather narrow fusiform ascospores in Pseudoastrosphaeriellaceae. Eight strains of Striatiguttulaceae formed two monophyletic sub-clades, which can be recognized as Longicorpus gen. nov. and Striatiguttula gen. nov. Morphologically, the genus Longicorpus can be differentiated from Striatiguttula by its elongated immersed ascomata and fusiform ascospores with relatively larger middle cells and paler end cells. Two new species Striatiguttula nypae and S. phoenicis, and one new combination, Longicorpus striataspora are introduced with morphological details, and phylogenetic relationships are discussed based on DNA sequence data.

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Research Article Mon, 1 Apr 2019 10:50:54 +0300
Neostagonosporella sichuanensis gen. et sp. nov. (Phaeosphaeriaceae, Pleosporales) on Phyllostachys heteroclada (Poaceae) from Sichuan Province, China https://mycokeys.pensoft.net/article/32458/ MycoKeys 46: 119-150

DOI: 10.3897/mycokeys.46.32458

Authors: Chun-Lin Yang, Xiu-Lan Xu, Dhanushka N. Wanasinghe, Rajesh Jeewon, Rungtiwa Phookamsak, Ying-Gao Liu, Li-Juan Liu, Kevin D. Hyde

Abstract: Neostagonosporella sichuanensis sp. nov. was found on Phyllostachys heteroclada collected from Sichuan Province in China and is introduced in a new genus Neostagonosporella gen. nov. in this paper. Evidence for the placement of the new taxon in the family Phaeosphaeriaceae is supported by morphology and phylogenetic analysis of a combined LSU, SSU, ITS and TEF 1-α DNA sequence dataset. Maximum-likelihood, maximum-parsimony and Bayesian inference phylogenetic analyses support Neostagonosporella as a distinct genus within this family. The new genus is compared with related genera of Phaeosphaeriaceae and full descriptions and illustrations are provided. Neostagonosporella is characterised by its unique suite of characters, such as multiloculate ascostromata and cylindrical to fusiform, transversely multiseptate, straight or curved ascospores, which are widest at the central cells. Conidiostromata are multiloculate, fusiform to long fusiform or rhomboid, with two types conidia; macroconidia vermiform or subcylindrical to cylindrical, transversely multiseptate, sometimes curved, almost equidistant between septa and microconidia oval, ellipsoidal or long ellipsoidal, aseptate, rounded at both ends. An updated phylogeny of the Phaeosphaeriaceae based on multigene analysis is provided.

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Research Article Mon, 18 Feb 2019 09:18:44 +0200
Sulcispora supratumida sp. nov. (Phaeosphaeriaceae, Pleosporales) on Anthoxanthum odoratum from Italy https://mycokeys.pensoft.net/article/27729/ MycoKeys 38: 35-46

DOI: 10.3897/mycokeys.38.27729

Authors: Indunil C. Senanayake, Rajesh Jeewon, Erio Camporesi, Kevin D. Hyde, Yu-Jia Zeng, Sheng-Li Tian, Ning Xie

Abstract: Sulcispora is typified by S. pleurospora. We collected a sulcispora-like taxon on leaves of Anthoxanthum odoratum L. in Italy and obtained single ascospore isolates. Combined ITS, LSU, SSU and tef1 sequence analyses suggested that Sulcispora is placed in the family Phaeosphaeriaceae and a newly collected Sulcispora species is introduced here as S. supratumida sp. nov. Detailed descriptions and illustrations are provided for Sulcispora supratumida and it is compared with the type species, S. pleurospora.

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Research Article Tue, 7 Aug 2018 11:25:48 +0300
Neodendryphiella, a novel genus of the Dictyosporiaceae (Pleosporales) https://mycokeys.pensoft.net/article/27275/ MycoKeys 37: 19-38

DOI: 10.3897/mycokeys.37.27275

Authors: Isabel Iturrieta-González, Josepa Gené, Josep Guarro, Rafael F. Castañeda-Ruiz, Dania García

Abstract: In a survey of soil and herbivore dung microfungi in Mexico and Spain, several dendryphiella-like species were found. Phylogenetic analyses based on ITS and LSU sequences showed that these fungi belonged to the family Dictyosporiaceae (Pleosporales) and represent an undescribed monophyletic lineage distant from Dendryphiella. Therefore, the genus Neodendryphiella is proposed to accommodate three new species, N. mali, N. michoacanensis and N. tarraconensis. The novel genus shares morphological features with Dendryphiella such as differentiated conidiophores and polytretic integrated conidiogenous cells, that produce acropetal branched chains of conidia. Neodendryphiella differs in the absence of nodulose conidiophores bearing conidiogenous cells with pores surrounded by a thickened and darkened wall, typical features in the conidiogenous apparatus of Dendryphiella. In addition, the phylogenetic and morphological analysis of several reference strains of different Dendryphiella species, available for comparison, support the proposal of D. variabilis sp. nov., which mainly differs from the other species of the genus by having conidia up to 7 septa and highlight that D. vinosa and D. infuscans are obscure species that require further taxonomic review.

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Research Article Thu, 26 Jul 2018 08:52:32 +0300
Two novel species of Neoaquastroma (Parabambusicolaceae, Pleosporales) with their phoma-like asexual morphs https://mycokeys.pensoft.net/article/25124/ MycoKeys 34: 47-62

DOI: 10.3897/mycokeys.34.25124

Authors: Chayanard Phukhamsakda, Darbhe J. Bhat, Sinang Hongsanan, Jian-Chu Xu, Marc Stadler, Kevin D. Hyde

Abstract: The monotypic genus Neoaquastroma (Parabambusicolaceae, Pleosporales) was introduced for a microfungus isolated from a collection of dried stems of a dicotyledonous plant in Thailand. In this paper, we introduce two novel species, N. bauhiniae and N. krabiense, in this genus. Their asexual morphs comprise conidiomata with aseptate and hyaline conidia. Neoaquastroma bauhiniae has ascomata, asci and ascospores that are smaller than those of N. krabiense. Descriptions and illustrations of N. bauhiniae and N. krabiense are provided and the two species compared with the type species of the genus, N. guttulatum. Evidence for the introduction of the new taxa is also provided from phylogenetic analysis of a combined dataset of partial LSU, SSU, ITS and tef1 sequence data. The phylogenetic analysis revealed a distinct lineage for N. bauhiniae and N. krabiense within the family Parabambusicolaceae.

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Research Article Wed, 23 May 2018 10:08:03 +0300