Latest Articles from MycoKeys Latest 4 Articles from MycoKeys https://mycokeys.pensoft.net/ Fri, 29 Mar 2024 10:08:07 +0200 Pensoft FeedCreator https://mycokeys.pensoft.net/i/logo.jpg Latest Articles from MycoKeys https://mycokeys.pensoft.net/ Two new Trichoderma species (Hypocreales, Hypocreaceae) isolated from decaying tubers of Gastrodia elate https://mycokeys.pensoft.net/article/109404/ MycoKeys 99: 187-207

DOI: 10.3897/mycokeys.99.109404

Authors: Chuwen Ye, Tingting Jing, Yuru Sha, Minghe Mo, Zefen Yu

Abstract: Species of Trichoderma are widely distributed around the world. In this study, two new species in Trichoderma, named as T. albidum and T. variegatum, were introduced and illustrated. These species were isolated from diseased tubers of Gastrodia elata in China and identified based on morphological characteristics and multi-gene sequence analyses of three loci that is the internal transcribed spacer regions of the ribosomal DNA (ITS), the translation elongation factor 1-α encoding gene (tef1-α) and the gene encoding the second largest nuclear RNA polymerase subunit (rpb2). Distinctions between the new species and their close relatives were discussed. According to results of the phylogenetic analyses, T. albidum belonged to the Harzianum clade and T. variegatum are grouped with species of the Spirale clade. The expansion of two clades provided research foundations for the prevention and control of tuber diseases in G. elata.

HTML

XML

PDF

]]>
Research Article Fri, 8 Sep 2023 08:30:42 +0300
Three new species of Trichoderma (Hypocreales, Hypocreaceae) from soils in China https://mycokeys.pensoft.net/article/101635/ MycoKeys 97: 21-40

DOI: 10.3897/mycokeys.97.101635

Authors: Rui Zhao, Li-Juan Mao, Chu-Long Zhang

Abstract: Trichoderma spp. are diverse fungi with wide distribution. In this study, we report on three new species of Trichoderma, namely T. nigricans, T. densissimum and T. paradensissimum, collected from soils in China. Their phylogenetic position of these novel species was determined by analyzing the concatenated sequences of the second largest nuclear RNA polymerase subunit encoding gene (rpb2) and the translation elongation factor 1– alpha encoding gene (tef1). The results of the phylogenetic analysis showed that each new species formed a distinct clade: T. nigricans is a new member of the Atroviride Clade, and T. densissimum and T. paradensissimum belong to the Harzianum Clade. A detailed description of the morphology and cultural characteristics of the newly discovered Trichoderma species is provided, and these characteristics were compared with those of closely related species to better understand the taxonomic relationships within the Trichoderma.

HTML

XML

PDF

]]>
Research Article Tue, 2 May 2023 11:44:55 +0300
Five new species of Trichoderma from moist soils in China https://mycokeys.pensoft.net/article/76085/ MycoKeys 87: 133-157

DOI: 10.3897/mycokeys.87.76085

Authors: Guang-Zhi Zhang, He-Tong Yang, Xin-Jian Zhang, Fang-Yuan Zhou, Xiao-Qing Wu, Xue-Ying Xie, Xiao-Yan Zhao, Hong-Zi Zhou

Abstract: Trichoderma isolates were collected from moist soils near a water source in different areas of China. ITS sequences were submitted to MIST (Multiloci Identification System for Trichoderma) and meets the Trichoderma [ITS76] standard. Combined analyses of phylogenetic analyses of both phylograms (tef1-α and rpb2) and morphological characteristics, revealed five new species of Trichoderma, namely Trichoderma hailarense, T. macrofasciculatum, T. nordicum, T. shangrilaense and T. vadicola. Phylogenetic analyses showed T. macrofasciculatum and T. shangrilaense belong to the Polysporum clade, T. hailarense, while T. nordicum and T. vadicola belong to the Viride clade. Each new taxon formed a distinct clade in phylogenetic analysis and have unique sequences of tef1-α and rpb2 that meet the Trichoderma new species standard. The conidiation of T. macrofasciculatum typically appeared in white pustules in concentric rings on PDA or MEA and its conidia had one or few distinctly verrucose. Conidiophores of T. shangrilaense are short and rarely branched, phialides usually curved and irregularly disposed. The aerial mycelium of T. hailarense and T. vadicola formed strands to floccose mat, conidiation tardy and scattered in tufts, conidiophores repeatedly rebranching in dendriform structure. The phialides of T. nordicum lageniform are curved on PDA and its conidia are globose to obovoidal and large.

HTML

XML

PDF

]]>
Research Article Thu, 17 Feb 2022 11:58:07 +0200
More pieces to a huge puzzle: Two new Escovopsis species from fungus gardens of attine ants https://mycokeys.pensoft.net/article/30951/ MycoKeys 46: 97-118

DOI: 10.3897/mycokeys.46.30951

Authors: Quimi Vidaurre Montoya, Maria Jesus Sutta Martiarena, Danilo Augusto Polezel, Sérgio Kakazu, Andre Rodrigues

Abstract: Escovopsis (Ascomycota: Hypocreales, Hypocreaceae) is the only known parasite of the mutualistic fungi cultivated by fungus-growing ants (Formicidae: Myrmicinae: Attini: Attina, the “attines”). Despite its ecological role, the taxonomy and systematics of Escovopsis have been poorly addressed. Here, based on morphological and phylogenetic analyses with three molecular markers (internal transcribed spacer, large subunit ribosomal RNA and the translation elongation factor 1-alpha), we describe Escovopsis clavatus and E. multiformis as new species isolated from fungus gardens of Apterostigma ant species. Our analysis shows that E. clavatus and E. multiformis belong to the most derived Escovopsis clade, whose main character is the presence of conidiophores with vesicles. Nevertheless, the most outstanding feature of both new species is the presence of a swollen region in the central hypha of the conidiophore named swollen cell, which is absent in all previously described Escovopsis species. The less derived Escovopsis clades lack vesicles and their phylogenetic position within the Hypocreaceae still remains unclear. Considering the high genetic diversity in Escovopsis, the description of these new species adds barely two pieces to a huge taxonomic puzzle; however, this discovery is an important piece for building the systematics of this group of fungi.

HTML

XML

PDF

]]>
Research Article Mon, 18 Feb 2019 09:48:30 +0200