Latest Articles from MycoKeys Latest 9 Articles from MycoKeys https://mycokeys.pensoft.net/ Fri, 29 Mar 2024 08:14:16 +0200 Pensoft FeedCreator https://mycokeys.pensoft.net/i/logo.jpg Latest Articles from MycoKeys https://mycokeys.pensoft.net/ Four new endophytic species of Diaporthe (Diaporthaceae, Diaporthales) isolated from Cameroon https://mycokeys.pensoft.net/article/110043/ MycoKeys 99: 319-362

DOI: 10.3897/mycokeys.99.110043

Authors: Christopher Lambert, Lena Schweizer, Blondelle Matio Kemkuignou, Elodie Gisèle M. Anoumedem, Simeon F. Kouam, Yasmina Marin-Felix

Abstract: The genus Diaporthe (Diaporthaceae, Diaporthales) is a large group of fungi frequently reported as phytopathogens, with ubiquitous distribution across the globe. Diaporthe have traditionally been characterized by the morphology of their ana- and teleomorphic state, revealing a high degree of heterogeneity as soon as DNA sequencing was utilized across the different members of the group. Their relevance for biotechnology and agriculture attracts the attention of taxonomists and natural product chemists alike in context of plant protection and exploitation for their potential to produce bioactive secondary metabolites. While more than 1000 species are described to date, Africa, as a natural habitat, has so far been under-sampled. Several endophytic fungi belonging to Diaporthe were isolated from different plant hosts in Cameroon over the course of this study. Phylogenetic analyses based on DNA sequence data of the internal transcribed spacer region and intervening 5.8S nrRNA gene, and partial fragments of the calmodulin, beta-tubulin, histone and the translation elongation factor 1-α genes, demonstrated that these isolates represent four new species, i.e. D. brideliae, D. cameroonensis, D. pseudoanacardii and D. rauvolfiae. Moreover, the description of D. isoberliniae is here emended, now incorporating the morphology of beta and gamma conidia produced by two of our endophytic isolates, which had never been documented in previous records. Moreover, the paraphyletic nature of the genus is discussed and suggestions are made for future revision of the genus.

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Research Article Mon, 23 Oct 2023 18:19:57 +0300
Studies of Diaporthe (Diaporthaceae, Diaporthales) species associated with plant cankers in Beijing, China, with three new species described https://mycokeys.pensoft.net/article/104156/ MycoKeys 98: 59-86

DOI: 10.3897/mycokeys.98.104156

Authors: Yukun Bai, Lu Lin, Meng Pan, Xinlei Fan

Abstract: The genus Diaporthe (Diaporthaceae, Diaporthales) comprises endophytes, pathogens and saprophytes, inhabiting a wide range of woody hosts and resulting in serious canker disease. To determine the diversity of Diaporthe species associated with canker disease of host plants in Beijing, China, a total of 35 representative strains were isolated from 18 host genera. Three novel species (D. changpingensis, D. diospyrina and D. ulmina) and four known species (D. corylicola, D. donglingensis, D. eres and D. rostrata) were identified, based on morphological comparison and phylogenetic analyses using partial ITS, cal, his3, tef1-α and tub2 loci. These results provide an understanding of the taxonomy of Diaporthe species associated with canker diseases in Beijing, China.

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Research Article Mon, 29 May 2023 17:57:22 +0300
Two new species of Diaporthe (Diaporthaceae, Diaporthales) in China https://mycokeys.pensoft.net/article/98969/ MycoKeys 95: 209-228

DOI: 10.3897/mycokeys.95.98969

Authors: Ya-Quan Zhu, Chun-Yan Ma, Han Xue, Chun-Gen Piao, Yong Li, Ning Jiang

Abstract: Species of Diaporthe have been reported as plant endophytes, pathogens and saprobes on a wide range of plant hosts. Strains of Diaporthe were isolated from leaf spots of Smilax glabra and dead culms of Xanthium strumarium in China, and identified based on morphology and molecular phylogenetic analyses of combined internal transcribed spacer region (ITS), calmodulin (cal), histone H3 (his3), translation elongation factor 1-alpha (tef1) and β-tubulin (tub2) loci. As a result, two new species named Diaporthe rizhaoensis and D. smilacicola are identified, described and illustrated in the present study.

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Research Article Wed, 1 Mar 2023 14:17:38 +0200
Four new species of Diaporthe (Diaporthaceae, Diaporthales) from forest plants in China https://mycokeys.pensoft.net/article/84970/ MycoKeys 91: 25-47

DOI: 10.3897/mycokeys.91.84970

Authors: Lingxue Cao, Dun Luo, Wu Lin, Qin Yang, Xiaojun Deng

Abstract: Species of Diaporthe inhabit a wide range of plant hosts as plant pathogens, endophytes and saprobes. During trips to collect forest pathogens in Beijing, Jiangxi, Shaanxi and Zhejiang Provinces in China, 16 isolates of Diaporthe were obtained from branch cankers and leaf spots. These isolates were studied by applying a polyphasic approach including morphological, cultural data, and phylogenetic analyses of the nuclear ribosomal internal transcribed spacer (ITS), calmodulin (cal), histone H3 (his3), partial translation elongation factor-1α (tef-1α) and β-tubulin (tub2) loci. Results revealed four new taxa, D. celticola, D. meliae, D. quercicola, D. rhodomyrti spp. nov. and two known species, D. eres and D. multiguttulata.

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Research Article Wed, 6 Jul 2022 11:12:42 +0300
Characterization of Diaporthe species on Camellia oleifera in Hunan Province, with descriptions of two new species https://mycokeys.pensoft.net/article/71701/ MycoKeys 84: 15-33

DOI: 10.3897/mycokeys.84.71701

Authors: Qin Yang, Jie Tang, Guo Y. Zhou

Abstract: Tea-oil tree (Camellia oleifera Abel.) is an important edible oil woody plant with a planting area over 3,800,000 hectares in southern China. Species of Diaporthe inhabit a wide range of plant hosts as plant pathogens, endophytes and saprobes. At present, relatively little is known about the taxonomy and genetic diversity of Diaporthe on C. oleifera. Here, we conducted an extensive field survey in Hunan Province in China to identify and characterise Diaporthe species associated with tea-oil leaf spots. As a result, eleven isolates of Diaporthe were obtained from symptomatic C. oleifera leaves. These isolates were studied by applying a polyphasic approach including morphological and phylogenetic analyses of partial ITS, cal, his3, tef1 and tub2 gene regions. Two new Diaporthe species (D. camelliae-oleiferae and D. hunanensis) were proposed and described herein, and C. oleifera was revealed to be new host records of D. hubeiensis and D. sojae. This study indicated there is a potential of more undiscovered Diaporthe species from C. oleifera in China.

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Research Article Mon, 18 Oct 2021 11:45:37 +0300
Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China https://mycokeys.pensoft.net/article/63816/ MycoKeys 80: 77-90

DOI: 10.3897/mycokeys.80.63816

Authors: Xianhong Wang, Yashuang Guo, Yamin Du, Ziling Yang, Xinzhong Huang, Ni Hong, Wenxing Xu, Guoping Wang

Abstract: Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

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Research Article Tue, 18 May 2021 08:11:22 +0300
Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China https://mycokeys.pensoft.net/article/60878/ MycoKeys 78: 49-77

DOI: 10.3897/mycokeys.78.60878

Authors: Shengting Huang, Jiwen Xia, Xiuguo Zhang, WenXiu Sun

Abstract: Species of Diaporthe have often been reported as plant pathogens, endophytes or saprobes, commonly isolated from a wide range of plant hosts. Sixteen strains isolated from species of ten host genera in Yunnan Province, China, represented three new species of Diaporthe, D. chrysalidocarpi, D. machili and D. pometiae as well as five known species D. arecae, D. hongkongensis, D. middletonii, D. osmanthi and D. pandanicola. Morphological comparisons with known species and DNA-based phylogenies based on the analysis of a multigene (ITS, TUB, TEF, CAL and HIS) dataset support the establishment of the new species. This study reveals that a high species diversity of Diaporthe with wide host ranges occur in tropical rainforest in Yunnan Province, China.

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Research Article Fri, 19 Feb 2021 15:35:25 +0200
Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species https://mycokeys.pensoft.net/article/59852/ MycoKeys 77: 65-95

DOI: 10.3897/mycokeys.77.59852

Authors: Wenxiu Sun, Shengting Huang, Jiwen Xia, Xiuguo Zhang, Zhuang Li

Abstract: Diaporthe species have often been reported as plant pathogens, endophytes and saprophytes, commonly isolated from a wide range of infected plant hosts. In the present study, twenty strains obtained from leaf spots of twelve host plants in Yunnan Province of China were isolated. Based on a combination of morphology, culture characteristics and multilocus sequence analysis of the rDNA internal transcribed spacer region (ITS), translation elongation factor 1-α (TEF), β-tubulin (TUB), calmodulin (CAL), and histone (HIS) genes, these strains were identified as eight new species: Diaporthe camelliae-sinensis, D. grandiflori, D. heliconiae, D. heterostemmatis, D. litchii, D. lutescens, D. melastomatis, D. pungensis and two previously described species, D. subclavata and D. tectonendophytica. This study showed high species diversity of Diaporthe in tropical rain forests and its hosts in south-western China.

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Review Article Thu, 14 Jan 2021 16:58:23 +0200
High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described https://mycokeys.pensoft.net/article/26914/ MycoKeys 39: 97-149

DOI: 10.3897/mycokeys.39.26914

Authors: Qin Yang, Xin-Lei Fan, Vladimiro Guarnaccia, Cheng-Ming Tian

Abstract: Diaporthe species have often been reported as important plant pathogens, saprobes and endophytes on a wide range of plant hosts. Although several Diaporthe species have been recorded in China, little is known about species able to infect forest trees. Therefore, extensive surveys were recently conducted in Beijing, Heilongjiang, Jiangsu, Jiangxi, Shaanxi and Zhejiang Provinces. The current results emphasised on 15 species from 42 representative isolates involving 16 host genera using comparisons of DNA sequence data for the nuclear ribosomal internal transcribed spacer (ITS), calmodulin (cal), histone H3 (his3), partial translation elongation factor-1α (tef1) and β-tubulin (tub2) gene regions, as well as their morphological features. Three known species, D. biguttulata, D. eres and D. unshiuensis, were identified. In addition, twelve novel taxa were collected and are described as D. acerigena, D. alangii, D. betulina, D. caryae, D. cercidis, D. chensiensis, D. cinnamomi, D. conica, D. fraxinicola, D. kadsurae, D. padina and D. ukurunduensis. The current study improves the understanding of species causing diebacks on ecological and economic forest trees and provides useful information for the effective disease management of these hosts in China.

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Research Article Mon, 17 Sep 2018 09:24:14 +0300