Latest Articles from MycoKeys Latest 12 Articles from MycoKeys https://mycokeys.pensoft.net/ Fri, 29 Mar 2024 01:47:54 +0200 Pensoft FeedCreator https://mycokeys.pensoft.net/i/logo.jpg Latest Articles from MycoKeys https://mycokeys.pensoft.net/ Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China https://mycokeys.pensoft.net/article/95751/ MycoKeys 94: 51-71

DOI: 10.3897/mycokeys.94.95751

Authors: Li Lu, Samantha C. Karunarathna, Dong-qin Dai, Ruvishika S. Jayawardena, Nakarin Suwannarach, Saowaluck Tibpromma

Abstract: Coffee is one of the most important cash crops in Yunnan Province, China. Yunnan is ranked as the biggest producer of high-quality coffee in China. During surveys of microfungi from coffee plantations in Yunnan, six fungal strains that resemble Nigrogranaceae were collected. Multi-gene analyses of a combined SSU-LSU-ITS-rpb2-tef1-α sequence data matrix were used to infer the phylogenetic position of the new species in Nigrograna while morphological characteristics were used to deduce the taxonomic position of the new species. Six fungal strains isolated from decaying branches of Coffea arabica represent three new saprobic species in Nigrograna. The three new species, N. asexualis, N. coffeae, and N. puerensis, are described with full (macro and micro characteristics) descriptions, illustrations, and a phylogenetic tree that shows the phylogenetic position of new taxa.

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Research Article Mon, 12 Dec 2022 10:40:56 +0200
Pleocatenata chiangraiensis gen. et. sp. nov. (Pleosporales, Dothideomycetes) from medicinal plants in northern Thailand https://mycokeys.pensoft.net/article/79433/ MycoKeys 87: 77-98

DOI: 10.3897/mycokeys.87.79433

Authors: Ya-Ru Sun, Ning-Guo Liu, Kevin D. Hyde, Ruvishika S. Jayawardena, Yong Wang

Abstract: Pleocatenata, a new genus, is introduced with its type species, Pleocatenata chiangraiensis, which was isolated from withered twigs of two medicinal plants, Clerodendrum quadriloculare (Blanco) Merr (Verbenaceae) and Tarenna stellulata (Hook.f.) Ridl (Rubiaceae) in northern Thailand. The genus is characterized by mononematous, septate, brown or dark brown conidiophores, monotretic conidiogenous cells and catenate, obclavate, olivaceous to blackish brown conidia. Phylogenetic analysis of combined LSU, SSU, tef1-α, rpb2 and ITS sequence data showed Pleocatenata forms a distinct phylogenetic lineage in Pleosporales, Dothideomycetes. Therefore, we treat Pleocatenata as Pleosporales genera incertae sedis based on morphology and phylogenetic analyses. Descriptions and illustrations of the new taxa are provided, and it is compared with morphologically similar genera.

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Research Article Fri, 11 Feb 2022 10:19:34 +0200
Novel saprobic Hermatomyces species (Hermatomycetaceae, Pleosporales) from China (Yunnan Province) and Thailand https://mycokeys.pensoft.net/article/67973/ MycoKeys 82: 57-79

DOI: 10.3897/mycokeys.82.67973

Authors: Guang-Cong Ren, Dhanushka N. Wanasinghe, Jutamart Monkai, Peter E. Mortimer, Kevin D. Hyde, Jian-Chu Xu, Aimin Pang, Heng Gui

Abstract: During our survey of the diversity of woody litter fungi in China and Thailand, three Hermatomyces species were collected from dead woody twigs of Dipterocarpus sp. (Dipterocarpaceae) and Ehretia acuminata (Boraginaceae). Both morphology and multigene analyses revealed two taxa as new species (Hermatomyces turbinatus and H. jinghaensis) and the remaining collections as new records of H. sphaericus. Hermatomyces turbinatus is characterized by 1) dimorphic conidia, having circular to oval lenticular conidia and 2) turbinate conidia consisting of two columns with two septa composed of 2–3 cells in each column. Hermatomyces jinghaensis is characterized by dimorphic conidia, having circular to oval lenticular conidia and clavate or subcylindrical to cylindrical conidia and consisting of one or two columns with 6–8 cells in each column. Phylogenetic analyses of combined LSU, ITS, tub2, tef1-α and rpb2 sequence data supports the placement of these new taxa within Hermatomycetaceae with high statistical support.

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Research Article Mon, 9 Aug 2021 15:36:43 +0300
Cryptic diversity found in Didymellaceae from Australian native legumes https://mycokeys.pensoft.net/article/60063/ MycoKeys 78: 1-20

DOI: 10.3897/mycokeys.78.60063

Authors: Elizabeth C. Keirnan, Yu Pei Tan, Matthew H. Laurence, Allison A. Mertin, Edward C. Y. Liew, Brett A. Summerell, Roger G. Shivas

Abstract: Ascochyta koolunga (Didymellaceae, Pleosporales) was first described in 2009 (as Phoma koolunga) and identified as the causal agent of Ascochyta blight of Pisum sativum (field pea) in South Australia. Since then A. koolunga has not been reported anywhere else in the world, and its origins and occurrence on other legume (Fabaceae) species remains unknown. Blight and leaf spot diseases of Australian native, pasture and naturalised legumes were studied to investigate a possible native origin of A. koolunga. Ascochyta koolunga was not detected on native, naturalised or pasture legumes that had leaf spot symptoms, in any of the studied regions in southern Australia, and only one isolate was recovered from P. sativum. However, we isolated five novel species in the Didymellaceae from leaf spots of Australian native legumes from commercial field pea regions throughout southern Australia. The novel species were classified on the basis of morphology and phylogenetic analyses of the internal transcribed spacer region and part of the RNA polymerase II subunit B gene region. Three of these species, Nothophoma garlbiwalawarda sp. nov., Nothophoma naiawu sp. nov. and Nothophoma ngayawang sp. nov., were isolated from Senna artemisioides. The other species described here are Epicoccum djirangnandiri sp. nov. from Swainsona galegifolia and Neodidymelliopsis tinkyukuku sp. nov. from Hardenbergia violacea. In addition, we report three new host-pathogen associations in Australia, namely Didymella pinodes on S. artemisioides and Vicia cracca, and D. lethalis on Lathyrus tingitanus. This is also the first report of Didymella prosopidis in Australia.

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Research Article Mon, 8 Feb 2021 16:14:10 +0200
Multi-gene phylogenetic evidence suggests Dictyoarthrinium belongs in Didymosphaeriaceae (Pleosporales, Dothideomycetes) and Dictyoarthrinium musae sp. nov. on Musa from Thailand https://mycokeys.pensoft.net/article/55493/ MycoKeys 71: 101-118

DOI: 10.3897/mycokeys.71.55493

Authors: Binu C. Samarakoon, Dhanushka N. Wanasinghe, Milan C. Samarakoon, Rungtiwa Phookamsak, Eric H.C. McKenzie, Putarak Chomnunti, Kevin D. Hyde, Saisamorn Lumyong, Samantha C. Karunarathna

Abstract: Dead leaves of Musa sp. (banana) were collected in northern Thailand during an investigation of saprobic fungi. Preliminary morphological observations revealed that three specimens belong to Dictyoarthrinium. Phylogenetic analyses of combined SSU, LSU, ITS and tef1-α sequence data revealed that Dictyoarthrinium forms a clade in Didymosphaeriaceae (Massarineae, Pleosporales, Dothideomycetes) sister to Spegazzinia. Based on contrasting morphological features with the extant taxa of Dictyoarthrinium, coupled with the multigene analyses, Dictyoarthrinium musae sp. nov. is introduced herein. Our study provides the first detailed molecular investigation for Dictyoarthrinium and supports its placement in Didymosphaeriaceae (Massarineae, Pleosporales, Dothideomycetes). Previously, Dictyoarthrinium was classified in Apiosporaceae (Xylariales, Sordariomycetes).

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Research Article Wed, 5 Aug 2020 08:19:27 +0300
Additions to Phaeosphaeriaceae (Pleosporales): Elongaticollum gen. nov., Ophiosphaerella taiwanensis sp. nov., Phaeosphaeriopsis beaucarneae sp. nov. and a new host record of Neosetophoma poaceicola from Musaceae https://mycokeys.pensoft.net/article/53674/ MycoKeys 70: 59-88

DOI: 10.3897/mycokeys.70.53674

Authors: Danushka S. Tennakoon, Kasun M. Thambugala, Dhanushka N. Wanasinghe, Eleni Gentekaki, Itthayakorn Promputtha, Chang-Hsin Kuo, Kevin D. Hyde

Abstract: A novel ascomycetous genus, Elongaticollum, occurring on leaf litter of Hedychium coronarium (Zingiberaceae) in Taiwan, is described and illustrated. Elongaticollum is characterized by dark brown to black, superficial, obpyriform, pycnidial conidiomata with a distinct elongate neck, and oval to oblong, hyaline, aseptate conidia. Phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian) of combined ITS, LSU, SSU and tef1-α sequence data revealed Elongaticollum as a distinct genus within the family Phaeosphaeriaceae with high statistical support. In addition, Ophiosphaerella taiwanensis and Phaeosphaeriopsis beaucarneae are described as new species from dead leaves of Agave tequilana and Beaucarnea recurvata (Asparagaceae), respectively. Neosetophoma poaceicola is reported as a new host record from dead leaves of Musa acuminata (Musaceae). Newly described taxa are compared with other similar species and comprehensive descriptions and micrographs are provided.

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Research Article Mon, 27 Jul 2020 08:22:50 +0300
Taxonomy and phylogenetic appraisal of Spegazzinia musae sp. nov. and S. deightonii (Didymosphaeriaceae, Pleosporales) on Musaceae from Thailand https://mycokeys.pensoft.net/article/52043/ MycoKeys 70: 19-37

DOI: 10.3897/mycokeys.70.52043

Authors: Binu C. Samarakoon, Rungtiwa Phookamsak, Dhanushka N. Wanasinghe, Putarak Chomnunti, Kevin D. Hyde, Eric H. C. Mckenzie, Itthayakorn Promputtha, Jian-Chu Xu, Yun-Ju Li

Abstract: Tropical plants host a range of fungal niches including endophytes, pathogens, epiphytes and saprobes. A study undertaken to discover the saprobic fungal species associated with Musa sp. (banana) from northern Thailand found two hyphomycetous taxa of Spegazzinia (Didymosphaeriaceae, Pleosporales). These were collected during the dry season and their morpho-molecular taxonomic relationships were investigated. Based on phylogenetic analysis of combined SSU, LSU, ITS and TEF1-α sequence data (77% ML, 0.99 BYPP) and contrasting morphological features to the sister taxon, we introduce Spegazzinia musae as a novel species from a decaying leaf of Musa sp. Details on the taxonomy, ecology and geographical distribution of Spegazzinia species are provided. In addition, we report S. deightonii as a new host record from Musa sp. Our data further validate the taxonomic placement of Spegazzinia in Didymosphaeriaceae.

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Research Article Tue, 21 Jul 2020 17:01:04 +0300
Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes) https://mycokeys.pensoft.net/article/47704/ MycoKeys 65: 49-99

DOI: 10.3897/mycokeys.65.47704

Authors: Lingwei Hou, Margarita Hernández-Restrepo, Johannes Zacharias Groenewald, Lei Cai, Pedro W. Crous

Abstract: Fungal communities play a crucial role in maintaining the health of managed and natural soil environments, which directly or indirectly affect the properties of plants and other soil inhabitants. As part of a Citizen Science Project initiated by the Westerdijk Fungal Biodiversity Institute and the Utrecht University Museum, which aimed to describe novel fungal species from Dutch garden soil, the diversity of Didymellaceae, which is one of the largest families in the Dothideomycetes was investigated. A preliminary analysis of the ITS and LSU sequences from the obtained isolates allowed the identification of 148 strains belonging to the family. Based on a multi-locus phylogeny of a combined ITS, LSU, rpb2 and tub2 alignment, and morphological characteristics, 20 different species were identified in nine genera, namely Ascochyta, Calophoma, Didymella, Juxtiphoma, Nothophoma, Paraboeremia, Phomatodes, Stagonosporopsis, and Xenodidymella. Several isolates confirmed to be ubiquitous plant pathogens or endophytes were for the first time identified from soil, such as Ascochyta syringae, Calophoma clematidis-rectae, and Paraboeremia litseae. Furthermore, one new genus and 12 novel species were described from soil: Ascochyta benningiorum sp. nov., Didymella degraaffiae sp. nov., D. kooimaniorum sp. nov., Juxtiphoma kolkmaniorum sp. nov., Nothophoma brennandiae sp. nov., Paraboeremia rekkeri sp. nov., P. truiniorum sp. nov., Stagonosporopsis stuijvenbergii sp. nov., S. weymaniae sp. nov., Vandijckomycella joseae gen. nov. et sp. nov., V. snoekiae sp. nov., and Xenodidymella weymaniae sp. nov. From the results of this study, soil was revealed to be a rich substrate for members of Didymellaceae, several of which were previously known only from diseased or apparently healthy plant hosts.

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Research Article Tue, 10 Mar 2020 11:18:56 +0200
Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica https://mycokeys.pensoft.net/article/35682/ MycoKeys 55: 59-86

DOI: 10.3897/mycokeys.55.35682

Authors: Martin Vohník, Ondřej Borovec, Zuzana Kolaříková, Radka Sudová, Martina Réblová

Abstract: Seagrasses provide invaluable ecosystem services yet very little is known about their root mycobiont diversity and distribution. Here we focused on the dominant Mediterranean seagrass Posidonia oceanica and assessed its root mycobiome at 32 localities covering most of the ecoregions in the NW Mediterranean Sea using light and scanning electron microscopy and tag-encoded 454-pyrosequencing. Microscopy revealed that the recently discovered dark septate endophytic association specific for P. oceanica is present at all localities and pyrosequencing confirmed that the P. oceanica root mycobiome is dominated by a single undescribed pleosporalean fungus, hitherto unknown from other hosts and ecosystems. Its numerous slow-growing isolates were obtained from surface-sterilised root segments at one locality and after prolonged cultivation, several of them produced viable sterile mycelium. To infer their phylogenetic relationships we sequenced and analysed the large (LSU) and small (SSU) subunit nrDNA, the ITS nrDNA and the DNA-directed RNA polymerase II (RPB2). The fungus represents an independent marine biotrophic lineage in the Aigialaceae (Pleosporales) and is introduced here as Posidoniomyces atricolor gen. et sp. nov. Its closest relatives are typically plant-associated saprobes from marine, terrestrial and freshwater habitats in Southeast Asia and Central America. This study expands our knowledge and diversity of the Aigialaceae, adds a new symbiotic lifestyle to this family and provides a formal name for the dominant root mycobiont of the dominant Mediterranean seagrass.

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Research Article Wed, 26 Jun 2019 11:37:54 +0300
Neostagonosporella sichuanensis gen. et sp. nov. (Phaeosphaeriaceae, Pleosporales) on Phyllostachys heteroclada (Poaceae) from Sichuan Province, China https://mycokeys.pensoft.net/article/32458/ MycoKeys 46: 119-150

DOI: 10.3897/mycokeys.46.32458

Authors: Chun-Lin Yang, Xiu-Lan Xu, Dhanushka N. Wanasinghe, Rajesh Jeewon, Rungtiwa Phookamsak, Ying-Gao Liu, Li-Juan Liu, Kevin D. Hyde

Abstract: Neostagonosporella sichuanensis sp. nov. was found on Phyllostachys heteroclada collected from Sichuan Province in China and is introduced in a new genus Neostagonosporella gen. nov. in this paper. Evidence for the placement of the new taxon in the family Phaeosphaeriaceae is supported by morphology and phylogenetic analysis of a combined LSU, SSU, ITS and TEF 1-α DNA sequence dataset. Maximum-likelihood, maximum-parsimony and Bayesian inference phylogenetic analyses support Neostagonosporella as a distinct genus within this family. The new genus is compared with related genera of Phaeosphaeriaceae and full descriptions and illustrations are provided. Neostagonosporella is characterised by its unique suite of characters, such as multiloculate ascostromata and cylindrical to fusiform, transversely multiseptate, straight or curved ascospores, which are widest at the central cells. Conidiostromata are multiloculate, fusiform to long fusiform or rhomboid, with two types conidia; macroconidia vermiform or subcylindrical to cylindrical, transversely multiseptate, sometimes curved, almost equidistant between septa and microconidia oval, ellipsoidal or long ellipsoidal, aseptate, rounded at both ends. An updated phylogeny of the Phaeosphaeriaceae based on multigene analysis is provided.

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Research Article Mon, 18 Feb 2019 09:18:44 +0200
Sulcispora supratumida sp. nov. (Phaeosphaeriaceae, Pleosporales) on Anthoxanthum odoratum from Italy https://mycokeys.pensoft.net/article/27729/ MycoKeys 38: 35-46

DOI: 10.3897/mycokeys.38.27729

Authors: Indunil C. Senanayake, Rajesh Jeewon, Erio Camporesi, Kevin D. Hyde, Yu-Jia Zeng, Sheng-Li Tian, Ning Xie

Abstract: Sulcispora is typified by S. pleurospora. We collected a sulcispora-like taxon on leaves of Anthoxanthum odoratum L. in Italy and obtained single ascospore isolates. Combined ITS, LSU, SSU and tef1 sequence analyses suggested that Sulcispora is placed in the family Phaeosphaeriaceae and a newly collected Sulcispora species is introduced here as S. supratumida sp. nov. Detailed descriptions and illustrations are provided for Sulcispora supratumida and it is compared with the type species, S. pleurospora.

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Research Article Tue, 7 Aug 2018 11:25:48 +0300
Neodendryphiella, a novel genus of the Dictyosporiaceae (Pleosporales) https://mycokeys.pensoft.net/article/27275/ MycoKeys 37: 19-38

DOI: 10.3897/mycokeys.37.27275

Authors: Isabel Iturrieta-González, Josepa Gené, Josep Guarro, Rafael F. Castañeda-Ruiz, Dania García

Abstract: In a survey of soil and herbivore dung microfungi in Mexico and Spain, several dendryphiella-like species were found. Phylogenetic analyses based on ITS and LSU sequences showed that these fungi belonged to the family Dictyosporiaceae (Pleosporales) and represent an undescribed monophyletic lineage distant from Dendryphiella. Therefore, the genus Neodendryphiella is proposed to accommodate three new species, N. mali, N. michoacanensis and N. tarraconensis. The novel genus shares morphological features with Dendryphiella such as differentiated conidiophores and polytretic integrated conidiogenous cells, that produce acropetal branched chains of conidia. Neodendryphiella differs in the absence of nodulose conidiophores bearing conidiogenous cells with pores surrounded by a thickened and darkened wall, typical features in the conidiogenous apparatus of Dendryphiella. In addition, the phylogenetic and morphological analysis of several reference strains of different Dendryphiella species, available for comparison, support the proposal of D. variabilis sp. nov., which mainly differs from the other species of the genus by having conidia up to 7 septa and highlight that D. vinosa and D. infuscans are obscure species that require further taxonomic review.

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Research Article Thu, 26 Jul 2018 08:52:32 +0300