Latest Articles from MycoKeys Latest 100 Articles from MycoKeys https://mycokeys.pensoft.net/ Fri, 29 Mar 2024 03:23:25 +0200 Pensoft FeedCreator https://mycokeys.pensoft.net/i/logo.jpg Latest Articles from MycoKeys https://mycokeys.pensoft.net/ Rostrupomyces, a new genus to accommodate Xerocomus sisongkhramensis, and a new Hemileccinum species (Xerocomoideae, Boletaceae) from Thailand https://mycokeys.pensoft.net/article/107935/ MycoKeys 103: 129-165

DOI: 10.3897/mycokeys.103.107935

Authors: Santhiti Vadthanarat, Bhavesh Raghoonundon, Saisamorn Lumyong, Olivier Raspé

Abstract: A new genus, Rostrupomyces is established to accommodate Xerocomus sisongkhramensis based on multiple protein-coding genes (atp6, cox3, tef1, and rpb2) analyses of a wide taxon sampling of Boletaceae. In our phylogeny, the new genus was sister to Rubinosporus in subfamily Xerocomoideae, phylogenetically distant from Xerocomus, which was highly supported as sister to Phylloporus in the same subfamily Xerocomoideae. Rostrupomyces is different from other genera in Boletaceae by the following combination of characters: rugulose to subrugulose pileus surface, white pores when young becoming pale yellow in age, subscabrous stipe surface scattered with granulose squamules, white basal mycelium, unchanging color in any parts, yellowish brown spore print, and broadly ellipsoid to ellipsoid, smooth basidiospores. In addition, Hemileccinum inferius, also from subfamily Xerocomoideae, is newly described. Detailed descriptions and illustrations of the new genus and new species are presented.

HTML

XML

PDF

]]>
Research Article Thu, 28 Mar 2024 18:08:02 +0200
Species diversity and taxonomy of Vararia (Russulales, Basidiomycota) with descriptions of six species from Southwestern China https://mycokeys.pensoft.net/article/118980/ MycoKeys 103: 97-128

DOI: 10.3897/mycokeys.103.118980

Authors: Yinglian Deng, Sana Jabeen, Changlin Zhao

Abstract: Vararia is a species-rich genus in the family Peniophoraceae and has been shown to be polyphyletic. In this study, sequences of ITS and LSU rRNA markers of the studied samples were generated and phylogenetic analyses were performed with the maximum likelihood, maximum parsimony, and Bayesian inference methods. Seventeen lineages including six new species from China, i.e., V. fissurata, V. lincangensis, V. punctata, V. isabellina, V. sinensis, and V. yaoshanensis were recognized, in which V. fissurata is characterized by the brittle basidiomata with pruinose and cracking hymenophore having white to olivaceous buff hymenial surface, the clamped generative hyphae, presence of the two types gloeocystidia; V. lincangensis is characterized by the simple-septa generative hyphae, and thick-walled skeletal hyphae, and ellipsoid basidiospores; V. punctata is delimited by its thin to slightly thick-walled generative hyphae, and thick-walled skeletal hyphae, present thick-walled, clavate to cylindrical gloeocystidia; V. isabellina is characterized by having the cream to isabelline to slightly brown hymenial surface, thin to slightly thick-walled generative hyphae, and sub-fusiform to navicular basidiospores; V. sinensis is distinguishable by its white to slightly pink hymenial surface, thick-walled skeletal hyphae, and sub-fusiform to navicular basidiospores; V. yaoshanensis is characterized by cream to pinkish buff to cinnamon-buff hymenial surface, slightly thick-walled generative hyphae, the presence of two types gloeocystidia, and slightly thick-walled, ellipsoid basidiospores. Phylogram based on the ITS+nLSU rDNA gene regions included nine genera within the family Peniophoraceae as Amylostereum, Asterostroma, Baltazaria, Dichostereum, Michenera, Peniophora, Scytinostroma and Vararia, in which the six new wood-inhabiting fungi species were grouped into genus Vararia. The phylogenetic tree inferred from the combined ITS and LSU tree sequences highlighted that V. fissurata was found to be the sister to V. ellipsospora with strong supports. Additionally, V. lincangensis was clustered with V. fragilis. Furthermore, V. punctata was retrieved as a sister to V. ambigua. Moreover, V. sinensis was grouped with five taxa as V. breviphysa, V. pirispora, V. fusispora, V. abortiphysa and V. insolita. The new species V. isabellina formed a monophyletic lineage, in which it was then grouped closely with V. daweishanensis, and V. gracilispora. In addition, V. yaoshanensis was found to be the sister to V. gallica with strong supports. The present results increased the knowledge of Vararia species diversity and taxonomy of corticioid fungi in China. An identification key to 17 species of Vararia in China is provided.

HTML

XML

PDF

]]>
Research Article Fri, 22 Mar 2024 10:29:13 +0200
Two novel species of arctic-alpine lichen-forming fungi (Ascomycota, Megasporaceae) from the Deosai Plains, Pakistan https://mycokeys.pensoft.net/article/113310/ MycoKeys 102: 285-299

DOI: 10.3897/mycokeys.102.113310

Authors: Muhammad Usman, Paul S. Dyer, Matthias Brock, Christopher M. Wade, Abdul Nasir Khalid

Abstract: Members of the lichen-forming fungal genus Oxneriaria are known to occur in cold polar and high altitudinal environments. Two new species, Oxneriaria crittendenii and O. deosaiensis, are now described from the high altitude Deosai Plains, Pakistan, based on phenotypic, multigene phylogenetic and chemical evidence. Phenotypically, O. crittendenii is characterised by orbicular light-brown thalli 1.5–5 cm across, spot tests (K, C, KC) negative, apothecia pruinose, hymenium initially blue then dark orange in response to Lugol’s solution. Oxneriaria deosaiensis is characterised by irregular areolate grey thalli 1.5–2 cm across, K test (light brown), KC test (dark brown), apothecia epruinose, hymenium initially blue then dark blue in response to Lugol’s solution. Both species share the same characters of thalli with black margins and polarilocular ascospores. The closest previously reported species, O. pruinosa, differs from O. crittendenii and O. deosaiensis in having non-lobate margins, thin thalline exciple (45–80 μm thick), short asci (55–80 × 25–42 μm) and K positive (yellow) and KC negative tests and divergent DNA sequence in the ITS, LSU and mt SSU regions. The newly-described Oxneriaria species add to growing evidence of the Deosai Plains as a region of important arctic-alpine biodiversity.

HTML

XML

PDF

]]>
Research Article Fri, 1 Mar 2024 18:21:56 +0200
Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora https://mycokeys.pensoft.net/article/116196/ MycoKeys 102: 155-181

DOI: 10.3897/mycokeys.102.116196

Authors: Łucja Ptach-Styn, Beata Guzow-Krzemińska, James C. Lendemer, Tor Tønsberg, Martin Kukwa

Abstract: Loxospora is a genus of crustose lichens containing 13 accepted species that can be separated into two groups, based on differences in secondary chemistry that correlate with differences in characters of the sexual reproductive structures (asci and ascospores). Molecular phylogenetic analyses recovered these groups as monophyletic and support their recognition as distinct genera that differ in phenotypic characters. Species containing 2’-O-methylperlatolic acid are transferred to the new genus, Chicitaea Guzow-Krzem., Kukwa & Lendemer and four new combinations are proposed: C. assateaguensis (Lendemer) Guzow-Krzem., Kukwa & Lendemer, C. confusa (Lendemer) Guzow-Krzem., Kukwa & Lendemer, C. cristinae (Guzow-Krzem., Łubek, Kubiak & Kukwa) Guzow-Krzem., Kukwa & Lendemer and C. lecanoriformis (Lumbsch, A.W. Archer & Elix) Guzow-Krzem., Kukwa & Lendemer. The remaining species produce thamnolic acid and represent Loxospora s.str. Haplotype analyses recovered sequences of L. elatina in two distinct groups, one corresponding to L. elatina s.str. and one to Pertusaria chloropolia, the latter being resurrected from synonymy of L. elatina and, thus, requiring the combination, L. chloropolia (Erichsen) Ptach-Styn, Guzow-Krzem., Tønsberg & Kukwa. Sequences of L. ochrophaea were found to be intermixed within the otherwise monophyletic L. elatina s.str. These two taxa, which differ in contrasting reproductive mode and overall geographic distributions, are maintained as distinct, pending further studies with additional molecular loci. Lectotypes are selected for Lecanora elatina, Pertusaria chloropolia and P. chloropolia f. cana. The latter is a synonym of Loxospora chloropolia. New primers for the amplification of mtSSU are also presented.

HTML

XML

PDF

]]>
Research Article Mon, 19 Feb 2024 16:32:21 +0200
Identification of two new species and a new host record of Distoseptispora (Distoseptisporaceae, Distoseptisporales, Sordariomycetes) from terrestrial and freshwater habitats in Southern China https://mycokeys.pensoft.net/article/115452/ MycoKeys 102: 83-105

DOI: 10.3897/mycokeys.102.115452

Authors: Xue-Mei Chen, Xia Tang, Jian Ma, Ning-Guo Liu, Saowaluck Tibpromma, Samantha C. Karunarathna, Yuan-Pin Xiao, Yong-Zhong Lu

Abstract: During our investigation of saprophytic fungi in Guizhou and Hainan provinces, China, three hyphomycetes were collected from terrestrial and freshwater habitats. Based on morphological characteristics and phylogenetic analyses of combined ITS, LSU, tef1-α, and rpb2 sequence data, two new species are introduced: Distoseptispora hainanensis and D. lanceolatispora. Additionally, one known species, D. tectonae, previously unreported from Edgeworthia chrysantha, is newly reported. Detailed descriptions, illustrations, and a phylogenetic tree to show the two new species and the new host record of Distoseptispora are provided. In addition, a checklist of Distoseptispora species with their locations, lifestyles, habitats, and hosts is provided.

HTML

XML

PDF

]]>
Research Article Fri, 9 Feb 2024 18:17:29 +0200
New species of Tropicoporus (Basidiomycota, Hymenochaetales, Hymenochaetaceae) from India, with a key to Afro-Asian Tropicoporus species https://mycokeys.pensoft.net/article/117067/ MycoKeys 102: 29-54

DOI: 10.3897/mycokeys.102.117067

Authors: Sugantha Gunaseelan, Kezhocuyi Kezo, Samantha C. Karunarathna, Erfu Yang, Changlin Zhao, Abdallah M. Elgorban, Saowaluck Tibpromma, Malarvizhi Kaliyaperumal

Abstract: The Inonotus linteus complex, predominantly reported from East Asia, Mesoamerica and Caribbean countries, was circumscribed into Tropicoporus as one of the new genera, based on morphological and phylogenetic data. The present paper describes four new species of Tropicoporus from India. Morphological characteristics and phylogenetic analyses, based on ITS and nLSU data, delimited the new species, which are named T. cleistanthicola, T. indicus, T. pseudoindicus and T. tamilnaduensis. The pairwise homoplasy index (PHI) test was done to confirm the distinctive nature of the new species. The traits of Indian species remain distinct from one another, except for the pileate basidiome with the mono-dimitic hyphal system, cystidioles and broadly ellipsoid basidiospores. Descriptions, illustrations, PHI test results and a phylogenetic tree to show the position of the new species are provided. In addition, an identification key to Tropicoporus in Asia and an African species is given.

HTML

XML

PDF

]]>
Research Article Mon, 5 Feb 2024 10:44:13 +0200
Diversity of Distoseptispora (Distoseptisporaceae) taxa on submerged decaying wood from the Red River in Yunnan, China https://mycokeys.pensoft.net/article/116096/ MycoKeys 102: 1-28

DOI: 10.3897/mycokeys.102.116096

Authors: Hong-Wei Shen, Dan-Feng Bao, Saranyaphat Boonmee, Yong-Zhong Lu, Xi-Jun Su, Yun-Xia Li, Zong-Long Luo

Abstract: The Red River Basin is located in the Indo-Burma biodiversity hotspot and is rich in lignicolous freshwater fungi, but no systematic research has been conducted. A systematic study on the species diversity of lignicolous freshwater fungi in the basin is ongoing. Seven distoseptispora-like specimens were collected from the Red River Basin in Yunnan. Phylogenetic analysis of ITS, LSU, tef1-α, and rpb2 genes and combined morphological data indicate that there are six distinct species of Distoseptispora, including two new species and four known species. Two new species were named D. suae and D. xinpingensis, and the four known species were D. bambusae, D. euseptata, D. obpyriformis and D. pachyconidia. This study provides detailed descriptions and illustrations of these six species and an updated phylogenetic backbone tree of Distoseptispora.

HTML

XML

PDF

]]>
Research Article Mon, 5 Feb 2024 10:43:53 +0200
Three novel species and new records of Kirschsteiniothelia (Kirschsteiniotheliales) from northern Thailand https://mycokeys.pensoft.net/article/115286/ MycoKeys 101: 347-370

DOI: 10.3897/mycokeys.101.115286

Authors: Antonio Roberto Gomes de Farias, Naghmeh Afshari, Veenavee S. Hittanadurage Silva, Johnny Louangphan, Omid Karimi, Saranyaphat Boonmee

Abstract: Kirschsteiniothelia (Kirschsteiniotheliales, Pleosporomycetidae) includes 39 saprobic species recorded from dead or decaying wood in terrestrial and freshwater habitats. This study focuses on exploring Kirschsteiniothelia diversity in woody litter in Thailand. Wood samples were collected from forest areas in Chiang Rai and Chiang Mai Provinces in Thailand and examined for fungal fructifications. Fungal isolates were obtained and their morphological and sequence data were characterised. Micromorphology associated with multilocus phylogeny of ITS, LSU and SSU sequence data identified three isolates as novel species (Kirschsteiniothelia inthanonensis, K. saprophytica and K. zizyphifolii) besides new host records for K. tectonae and K. xishuangbannaensis. The placement of the new taxa and records are supported by morphological illustrations, descriptions and molecular phylogenies and the implications of these findings are discussed. Our findings provide information for understanding Kirschsteiniothelia diversity and ecology.

HTML

XML

PDF

]]>
Research Article Fri, 2 Feb 2024 17:51:26 +0200
Hidden diversity of Pestalotiopsis and Neopestalotiopsis (Amphisphaeriales, Sporocadaceae) species allied with the stromata of entomopathogenic fungi in Taiwan https://mycokeys.pensoft.net/article/113090/ MycoKeys 101: 275-312

DOI: 10.3897/mycokeys.101.113090

Authors: Sheng-Yu Hsu, Yuan-Cheng Xu, Yu-Chen Lin, Wei-Yu Chuang, Shiou-Ruei Lin, Marc Stadler, Narumon Tangthirasunun, Ratchadawan Cheewangkoon, Hind A. AL-Shwaiman, Abdallah M. Elgorban, Hiran A. Ariyawansa

Abstract: Pestalotiopsis sensu lato, commonly referred to as pestalotiopsis-like fungi, exhibit a broad distribution and are frequently found as endophytes, saprobes and pathogens across various plant hosts. The taxa within pestalotiopsis-like fungi are classified into three genera viz. Pestalotiopsis, Pseudopestalotiopsis and Neopestalotiopsis, based on the conidial colour of their median cells and multi-locus molecular phylogenies. In the course of a biodiversity investigation focusing on pestalotiopsis-like fungi, a total of 12 fungal strains were identified. These strains were found to be associated with stromata of Beauveria, Ophiocordyceps and Tolypocladium in various regions of Taiwan from 2018 to 2021. These strains were evaluated morphologically and multi-locus phylogenetic analyses of the ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α) and tub2 (beta-tubulin) gene regions were conducted for genotyping. The results revealed seven well-classified taxa and one tentative clade in Pestalotiopsis and Neopestalotiopsis. One novel species, Pestalotiopsis manyueyuanani and four new records, N. camelliae-oleiferae, N. haikouensis, P. chamaeropis and P. hispanica, were reported for the first time in Taiwan. In addition, P. formosana and an unclassified strain of Neopestalotiopsis were identified, based on similarities of phylogeny and morphology. However, the data obtained in the present study suggest that the currently recommended loci for species delimitation of pestalotiopsis-like fungi do not deliver reliable or adequate resolution of tree topologies. The in-vitro mycelial growth rates of selected strains from these taxa had an optimum temperature of 25 °C, but growth ceased at 5 °C and 35 °C, while all the strains grew faster under alkaline than acidic or neutral pH conditions. This study provides the first assessment of pestalotiopsis-like fungi, associated with entomopathogenic taxa.

HTML

XML

PDF

]]>
Research Article Wed, 31 Jan 2024 11:56:12 +0200
Morphophylogenetic evidence reveals four new fungal species within Tetraplosphaeriaceae (Pleosporales, Ascomycota) from tropical and subtropical forest in China https://mycokeys.pensoft.net/article/113141/ MycoKeys 100: 171-204

DOI: 10.3897/mycokeys.100.113141

Authors: Xia Tang, Rajesh Jeewon, Yong-Zhong Lu, Abdulwahed Fahad Alrefaei, Ruvishika S. Jayawardena, Rong-Ju Xu, Jian Ma, Xue-Mei Chen, Ji-Chuan Kang

Abstract: Tetraplosphaeriaceae (Pleosporales, Ascomycota) is a family with many saprobes recorded from various hosts, especially bamboo and grasses. During a taxonomic investigation of microfungi in tropical and subtropical forest regions of Guizhou, Hainan and Yunnan provinces, China, several plant samples were collected and examined for fungi. Four newly discovered species are described based on morphology and evolutionary relationships with their allies inferred from phylogenetic analyses derived from a combined dataset of LSU, ITS, SSU, and tub2 DNA sequence data. Detailed illustrations, descriptions and taxonomic notes are provided for each species. The four new species of Tetraplosphaeriaceae reported herein are Polyplosphaeria guizhouensis, Polyplosphaeria hainanensis, Pseudotetraploa yunnanensis, and Tetraploa hainanensis. A checklist of Tetraplosphaeriaceae species with available details on their ecology is also provided.

HTML

XML

PDF

]]>
Research Article Wed, 6 Dec 2023 14:40:37 +0200
New species and new combinations in the genus Paraisaria (Hypocreales, Ophiocordycipitaceae) from the U.S.A., supported by polyphasic analysis https://mycokeys.pensoft.net/article/110959/ MycoKeys 100: 69-94

DOI: 10.3897/mycokeys.100.110959

Authors: Richard M. Tehan, Connor B. Dooley, Edward G. Barge, Kerry L. McPhail, Joseph W. Spatafora

Abstract: Molecular phylogenetic and chemical analyses, and morphological characterization of collections of North American Paraisaria specimens support the description of two new species and two new combinations for known species. P. cascadensis sp. nov. is a pathogen of Cyphoderris (Orthoptera) from the Pacific Northwest USA and P. pseudoheteropoda sp. nov. is a pathogen of cicadae (Hemiptera) from the Southeast USA. New combinations are made for Ophiocordyceps insignis and O. monticola based on morphological, ecological, and chemical study. A new cyclopeptide family proved indispensable in providing chemotaxonomic markers for resolving species in degraded herbarium specimens for which DNA sequencing is intractable. This approach enabled the critical linkage of a 142-year-old type specimen to a phylogenetic clade. The diversity of Paraisaria in North America and the utility of chemotaxonomy for the genus are discussed.

HTML

XML

PDF

]]>
Research Article Mon, 13 Nov 2023 19:00:03 +0200
Taxonomy and phylogeny of the genus Ganoderma (Polyporales, Basidiomycota) in Costa Rica https://mycokeys.pensoft.net/article/106810/ MycoKeys 100: 5-47

DOI: 10.3897/mycokeys.100.106810

Authors: Melissa Mardones, Julieta Carranza-Velázquez, Milagro Mata-Hidalgo, Xaviera Amador-Fernández, Hector Urbina

Abstract: Ganoderma species are well recognised by their significant role in the recycling of nutrients in ecosystems and by their production of secondary metabolites of medical and biotechnological importance. Ganoderma spp. are characterised by laccate and non-laccate, woody basidiocarps, polypore hymenophores and double-walled basidiospores generally with truncate apex. Despite the importance of this genus, its taxonomy is unclear and it includes several species’ complexes with few circumscribed species and incorrect geographic distributions. The aim of this work was to provide detailed morphological descriptions together with phylogenetic analyses using ITS sequences to confirm the presence of seven species of Ganoderma in Costa Rica: G. amazonense, G. applanatum s.l., G. australe, G. curtisii, G. ecuadorense, G. oerstedii and G. parvulum. This is the first study that integrates morphological and phylogenetic data of Ganoderma from Central America and a key of the neotropical species. Besides, the distribution range of G. curtisii, previously reported from North America and G. ecuadorense from South America, is expanded to Central America.

HTML

XML

PDF

]]>
Research Article Mon, 13 Nov 2023 17:55:12 +0200
Morphology, phylogeny and host specificity of two new Ophiocordyceps species belonging to the “zombie-ant fungi” clade (Ophiocordycipitaceae, Hypocreales) https://mycokeys.pensoft.net/article/107565/ MycoKeys 99: 269-296

DOI: 10.3897/mycokeys.99.107565

Authors: Dexiang Tang, Jing Zhao, Yingling Lu, Zhiqin Wang, Tao Sun, Zuoheng Liu, Hong Yu

Abstract: Species of the genus Ophiocordyceps, which include species able to manipulate the behaviour of ants, are known as the “zombie-ant fungi” and have attracted much attention over the last decade. They are widespread within tropical, subtropical and even temperate forests worldwide, with relatively few reports from subtropical monsoon evergreen broad-leaved forest. Fungal specimens have been collected from China, occurring on ants and producing hirsutella-like anamorphs. Based on a combination of morphological characters, phylogenetic analyses (LSU, SSU, TEF1a, RPB1 and RPB2) and ecological data, two new species, Ophiocordyceps tortuosa and O. ansiformis, are identified and proposed herein. Ophiocordyceps tortuosa and O. ansiformis are recorded on the same species of Colobopsis ant, based on phylogenetic analyses (COI), which may be sharing the same host. Ophiocordyceps tortuosa and O. ansiformis share the morphological character of producing lanceolate ascospores. They have typical characteristics distinguished from other species. The ascospore of O. tortuosa are tortuously arranged in the ascus and the ascospore of O. ansiformis have a structure like a handle-shape in the middle. Our molecular data also indicate that O. tortuosa and O. ansiformis are clearly distinct from other species.

HTML

XML

PDF

]]>
Research Article Mon, 16 Oct 2023 18:22:52 +0300
Phylogeny and species delimitations in the economically, medically, and ecologically important genus Samsoniella (Cordycipitaceae, Hypocreales) https://mycokeys.pensoft.net/article/106474/ MycoKeys 99: 227-250

DOI: 10.3897/mycokeys.99.106474

Authors: Yao Wang, Zhi-Qin Wang, Chinnapan Thanarut, Van-Minh Dao, Yuan-Bing Wang, Hong Yu

Abstract: Samsoniella is a ubiquitous genus of cosmopolitan arthropod-pathogenic fungi in the family Cordycipitaceae. The fungi have economic, medicinal, and ecological importance. Prior taxonomic studies of these fungi relied predominantly on phylogenetic inferences from five loci, namely, the nuclear ribosomal small and large subunits (nr SSU and nr LSU), the 3’ portion of translation elongation factor 1 alpha (3P_TEF), and RNA polymerase II subunits 1 and 2 (RPB1 and RPB2). Despite many new species being described, not all of the recognized species inside this group formed well-supported clades. Thus, the search for new markers appropriate for molecular phylogenetic analysis of Samsoniella remains a challenging problem. In our study, we selected the internal transcribed spacer regions of the rDNA (ITS rDNA) and seven gene regions, namely, 3P_TEF, the 5’ portion of translation elongation factor 1 alpha (5P_TEF), RPB1, RPB2, γ-actin (ACT), β-tubulin (TUB), and a gene encoding a minichromosome maintenance protein (MCM7), as candidate markers for species identification. Genetic divergence comparisons showed that the ITS, RPB2, ACT, and TUB sequences provided little valuable information with which to separate Samsoniella spp. In contrast, sequence data for 3P_TEF, 5P_TEF, RPB1, and MCM7 provided good resolution of Samsoniella species. The phylogenetic tree inferred from combined data (5P_TEF + 3P_TEF + RPB1 + MCM7) showed well-supported clades for Samsoniella and allowed for the delimitation of 26 species in this genus. The other two species (S. formicae and S. lepidopterorum) were not evaluated, as they had abundant missing data.

HTML

XML

PDF

]]>
Research Article Tue, 3 Oct 2023 20:42:19 +0300
New species of Mallocybe (Agaricales, Inocybaceae) from Pakistan, based on morphological and molecular evidence https://mycokeys.pensoft.net/article/86844/ MycoKeys 99: 171-186

DOI: 10.3897/mycokeys.99.86844

Authors: Malka Saba, Abdul Nasir Khalid, Samina Sarwar

Abstract: Within the family Inocybaceae, many species of Mallocybe have been reported, but there are only a few reports of this genus from Pakistan. In this study, six collections of Mallocybe were studied by morphological and phylogenetic methods. Phylogenetic analyses, based on sequence data from two different loci (ITS and LSU) using Maximum Likelihood and Maximum Parsimony methods, have been performed to infer species relationships within Mallocybe. Results indicated that these six collections encompass two new species of Mallocybe i.e. M. pakistanica and M. pinicola, from Pakistan. Their detailed morphological descriptions and illustrations are also provided. In addition, comparison with morphologically closely-related taxa is also discussed. Previously, only two species of this genus have been recorded from Pakistan and, with this addition, the total number of reported taxa of Mallocybe has been raised to four from Pakistan. A key to the described taxa of Mallocybe from Pakistan is also provided.

HTML

XML

PDF

]]>
Research Article Thu, 7 Sep 2023 17:15:52 +0300
Additions to Hohenbuehelia (Basidiomycota, Pleurotaceae): two new species and notes on H. tristis from northern Thailand https://mycokeys.pensoft.net/article/105317/ MycoKeys 99: 109-130

DOI: 10.3897/mycokeys.99.105317

Authors: Monthien Phonemany, Santhiti Vadthanarat, Bhavesh Raghoonundon, Naritsada Thongklang, Olivier Raspé

Abstract: Two new species and a first geographical record of Hohenbuehelia are described from Thailand. Macroscopic and microscopic descriptions with photoplates, as well as a multigene phylogeny are provided. Hohenbuehelia flabelliformis sp. nov. is recognised by large flabelliform basidiomata, densely villose yellowish-white pileus with white hairs near the point of attachment, basidiospores that mostly are ellipsoid in front view and phaseoliform in side view, the absence of cheilocystidia, and a trichoderm pileipellis. Hohenbuehelia lageniformis sp. nov. is characterised by fleshy basidiomata, velutinous pileus with whitish hairs near the point of attachment and the margin, elsewhere pale greyish-yellow and with only sparse white hairs, pale brown to light brown and mucilaginous context, subglobose basidiospores, lageniform cheilocystidia, an ixotrichoderm pileipellis, and the absence of pileoleptocystidia. Hohenbuehelia tristis is characterised by small creamy-white, spathuliform basidiomata that are larger than the type subspecies, minutely pubescent pileus with tiny greyish hairs that disappear when mature, leaving the surface glutinous, faintly translucent and shiny, ellipsoid to sub-ellipsoid basidiospores, lecythiform to sublageniform cheilocystidia, and an ixotrichoderm pileipellis. Hohenbuehelia tristis is recorded for Thailand for the first time. Based on the polymorphism observed in part of the nrLSU gene, the presence of two divergent lineages within H. tristis is discussed.

HTML

XML

PDF

]]>
Research Article Mon, 21 Aug 2023 09:41:22 +0300
Introduction of two novel species of Hymenopellis (Agaricales, Physalacriaceae) from Thailand https://mycokeys.pensoft.net/article/104517/ MycoKeys 98: 253-271

DOI: 10.3897/mycokeys.98.104517

Authors: Allen Grace T. Niego, Naritsada Thongklang, Kevin D. Hyde, Olivier Raspé

Abstract: Hymenopellis is the most diverse genus in the group of oudemansielloid/xeruloid taxa (Physalacriaceae). This genus has a worldwide distribution with records mostly from Europe and America. Asian taxa are least represented. In this paper on Hymenopellis from Thailand, two novel species are introduced, and a Hymenopellis collection affine to H. orientalis is described. Macro and micromorphological characters are described. Maximum likelihood and Bayesian phylogenetic analyses were performed on combined ITS and nrLSU regions to confirm taxonomical placement and infer the phylogenetic affinities of the studied species. Hymenopellis straminea sp. nov. is straw-yellow, with medium-sized basidiomata, abundant and diverse in form cheilocystidia, few, narrowly lageniform to fusiform pleurocystidia, and clamp connections at the lower part of the stipe. Hymenopellis utriformis sp. nov. has mostly utriform pleurocystidia and 2-spored basidia. In the inferred phylogenies, the new species from this study formed distinct clades well supported by bootstrap proportions and posterior probabilities. The studied specimen affine to H. orientalis produced 2-spored basidia whereas published descriptions of other specimens mention 4-spored basidia. Moreover, the genetic distance between ITS sequences of this specimen and that of a Hymenopellis orientalis specimen from GenBank was 1.30–2.57%. Therefore, the conspecificity of our specimen with H. orientalis is uncertain, and additional specimens are needed to fully confirm its identity.

HTML

XML

PDF

]]>
Research Article Mon, 24 Jul 2023 10:24:00 +0300
Outline, phylogenetic and divergence times analyses of the genus Haploporus (Polyporales, Basidiomycota): two new species are proposed https://mycokeys.pensoft.net/article/105684/ MycoKeys 98: 233-252

DOI: 10.3897/mycokeys.98.105684

Authors: Heng Zhao, Josef Vlasák, Yuan Yuan

Abstract: Haploporus species have a worldwide distribution and 27 species have been accepted. In this study, two new species, Haploporus crystallinus and H. dextrinoideus, are proposed from South America, based on the molecular fragments (ITS, LSU and mtSSU) and morphological evidence. Molecular clock analysis was performed and the result suggests that the ancestor of Polyporales originated between the Late Jurassic and Early Cretaceous period, with a mean stem of 159.8 Mya [95% higher posterior density (HPD) of 142.4–184.1 Mya] and the genus Haploporus occurred at a mean stem of 108.3 Mya (95% HPD of 88.5–128.2 Mya). In addition, most species of the genus are diversified between 60.5 Mya and 1.8 Mya, during the Paleogene to Neogene. A key to the accepted species of the genus Haploporus is provided.

HTML

XML

PDF

]]>
Research Article Mon, 24 Jul 2023 10:20:20 +0300
Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes) https://mycokeys.pensoft.net/article/102816/ MycoKeys 98: 167-220

DOI: 10.3897/mycokeys.98.102816

Authors: Zhi-Yuan Zhang, Xin Li, Wan-Hao Chen, Jian-Dong Liang, Yan-Feng Han

Abstract: As China’s urbanisation continues to advance, more people are choosing to live in cities. However, this trend has a significant impact on the natural ecosystem. For instance, the accumulation of keratin-rich substrates in urban habitats has led to an increase in keratinophilic microbes. Despite this, there is still a limited amount of research on the prevalence of keratinophilic fungi in urban areas. Fortunately, our group has conducted in-depth investigations into this topic since 2015. Through our research, we have discovered a significant amount of keratinophilic fungi in soil samples collected from various urban areas in China. In this study, we have identified and characterised 18 new species through the integration of morphological and phylogenetic analyses. These findings reveal the presence of numerous unexplored fungal taxa in urban habitats, emphasising the need for further taxonomic research in urban China.

HTML

XML

PDF

]]>
Research Article Thu, 29 Jun 2023 18:29:46 +0300
Diversity of Cladosporium (Cladosporiales, Cladosporiaceae) species in marine environments and report on five new species https://mycokeys.pensoft.net/article/101918/ MycoKeys 98: 87-111

DOI: 10.3897/mycokeys.98.101918

Authors: Wonjun Lee, Ji Seon Kim, Chang Wan Seo, Jun Won Lee, Sung Hyun Kim, Yoonhee Cho, Young Woon Lim

Abstract: Cladosporium species are cosmopolitan fungi, characterized by olivaceous or dark colonies with coronate conidiogenous loci and conidial hila with a central convex dome surrounded by a raised periclinal rim. Cladosporium species have also been discovered in marine environments. Although many studies have been performed on the application of marine originated Cladosporium species, taxonomic studies on these species are scarce. We isolated Cladosporium species from three under-studied habitats (sediment, seawater, and seaweed) in two districts including an intertidal zone in the Republic of Korea and the open sea in the Western Pacific Ocean. Based on multigenetic marker analyses (for the internal transcribed spacer, actin, and translation elongation factor 1), we identified fourteen species, of which five were found to represent new species. These five species were C. lagenariiforme sp. nov., C. maltirimosum sp. nov., C. marinum sp. nov. in the C. cladosporioides species complex, C. snafimbriatum sp. nov. in the C. herbarum species complex, and C. marinisedimentum sp. nov. in the C. sphaerospermum species complex. Morphological characteristics of the new species and aspects of differences with the already known species are described herein together with molecular data.

HTML

XML

PDF

]]>
Research Article Fri, 2 Jun 2023 16:13:54 +0300
Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China https://mycokeys.pensoft.net/article/103484/ MycoKeys 98: 19-35

DOI: 10.3897/mycokeys.98.103484

Authors: Feng Liu, Yu Deng, Fei-Hu Wang, Rajesh Jeewon, Qian Zeng, Xiu-Lan Xu, Ying-Gao Liu, Chun-Lin Yang

Abstract: The fungal genus Microcera consists of species mostly occurring as parasites of scale insects, but are also commonly isolated from soil or lichens. In the present study, we surveyed the diversity and assess the taxonomy of entomopathogenic fungi in Sichuan Province, China. Two new species of Microcera, viz. M. chrysomphaludis and M. pseudaulacaspidis, were isolated from scale insects colonising walnut (Juglans regia). Maximum Likelihood and Bayesian Inference analyses of ITS, LSU, tef1-α, rpb1, rpb2, acl1, act, tub2, cmdA and his3 sequence data provide evidence for the validity of the two species and their placement in Nectriaceae (Hypocreales). Microcera pseudaulacaspidis primarily differs from similar species by having more septate and smaller cylindrical macroconidia, as well as DNA sequence data. Meanwhile, Microcera chrysomphaludis has elliptical, one-septate ascospores with acute ends and cylindrical, slightly curved with 4–6 septate macroconidia up to 78 µm long. Morphological descriptions with illustrations of the novel species and DNA-based phylogeny generated from analyses of multigene dataset are also provided to better understand species relationships.

HTML

XML

PDF

]]>
Research Article Mon, 29 May 2023 17:55:50 +0300
Morphology and molecular analyses reveal three new species of Botryosphaeriales isolated from diseased plant branches in China https://mycokeys.pensoft.net/article/102653/ MycoKeys 97: 1-19

DOI: 10.3897/mycokeys.97.102653

Authors: Lu Lin, Yukun Bai, Meng Pan, Chengming Tian, Xinlei Fan

Abstract: The Botryosphaeriales represents an ecologically diverse group of fungi, comprising endophytes, saprobes, and plant pathogens. In this study, taxonomic analyses were conducted based on morphological characteristics and phylogenetic analyses of multi-gene sequence data from four loci (ITS, LSU, tef1-α, and tub2). Thirteen isolates obtained from Beijing and Yunnan Province were identified as seven species of Botryosphaeriales, including Aplosporella javeedii, Dothiorella alpina, Phaeobotryon aplosporum and Ph. rhois, and three previously undescribed species, namely Aplosporella yanqingensis, Dothiorella baihuashanensis, and Phaeobotryon platycladi. Additionally, the new records of Dothiorella alpina from the host species Populus szechuanica, Phaeobotryon aplosporum from Juglans mandshurica, and Phaeobotryon rhois from Populus alba var. pyramidalis are included.

HTML

XML

PDF

]]>
Research Article Wed, 26 Apr 2023 21:35:43 +0300
How, not if, is the question mycologists should be asking about DNA-based typification https://mycokeys.pensoft.net/article/102669/ MycoKeys 96: 143-157

DOI: 10.3897/mycokeys.96.102669

Authors: R. Henrik Nilsson, Martin Ryberg, Christian Wurzbacher, Leho Tedersoo, Sten Anslan, Sergei Põlme, Viacheslav Spirin, Vladimir Mikryukov, Sten Svantesson, Martin Hartmann, Charlotte Lennartsdotter, Pauline Belford, Maryia Khomich, Alice Retter, Natàlia Corcoll, Daniela Gómez Martinez, Tobias Jansson, Masoomeh Ghobad-Nejhad, Duong Vu, Marisol Sanchez-Garcia, Erik Kristiansson, Kessy Abarenkov

Abstract: Fungal metabarcoding of substrates such as soil, wood, and water is uncovering an unprecedented number of fungal species that do not seem to produce tangible morphological structures and that defy our best attempts at cultivation, thus falling outside the scope of the International Code of Nomenclature for algae, fungi, and plants. The present study uses the new, ninth release of the species hypotheses of the UNITE database to show that species discovery through environmental sequencing vastly outpaces traditional, Sanger sequencing-based efforts in a strongly increasing trend over the last five years. Our findings challenge the present stance of some in the mycological community – that the current situation is satisfactory and that no change is needed to “the code” – and suggest that we should be discussing not whether to allow DNA-based descriptions (typifications) of species and by extension higher ranks of fungi, but what the precise requirements for such DNA-based typifications should be. We submit a tentative list of such criteria for further discussion. The present authors hope for a revitalized and deepened discussion on DNA-based typification, because to us it seems harmful and counter-productive to intentionally deny the overwhelming majority of extant fungi a formal standing under the International Code of Nomenclature for algae, fungi, and plants.

HTML

XML

PDF

]]>
Forum Paper Mon, 10 Apr 2023 10:59:55 +0300
Pseudolepraria, a new leprose genus revealed in Ramalinaceae (Ascomycota, Lecanoromycetes, Lecanorales) to accommodate Lepraria stephaniana https://mycokeys.pensoft.net/article/98029/ MycoKeys 96: 97-112

DOI: 10.3897/mycokeys.96.98029

Authors: Martin Kukwa, Magdalena Kosecka, Agnieszka Jabłońska, Adam Flakus, Pamela Rodriguez-Flakus, Beata Guzow-Krzemińska

Abstract: The new genus Pseudolepraria Kukwa, Jabłońska, Kosecka & Guzow-Krzemińska is introduced to accommodate Lepraria stephaniana Elix, Flakus & Kukwa. Phylogenetic analyses of nucITS, nucLSU, mtSSU and RPB2 markers recovered the new genus in the family Ramalinaceae with strong support. The genus is characterised by its thick, unstratified thallus composed entirely of soredia-like granules, the presence of 4-O-methylleprolomin, salazinic acid, zeorin and unknown terpenoid, and its phylogenetic position. The new combination, P. stephaniana (Elix, Flakus & Kukwa) Kukwa, Jabłońska, Kosecka & Guzow-Krzemińska, is proposed.

HTML

XML

PDF

]]>
Research Article Fri, 24 Mar 2023 15:54:04 +0200
Five new species of Schizoporaceae (Basidiomycota, Hymenochaetales) from East Asia https://mycokeys.pensoft.net/article/99327/ MycoKeys 96: 25-56

DOI: 10.3897/mycokeys.96.99327

Authors: Qian-Xin Guan, Jing Huang, Jian Huang, Chang-Lin Zhao

Abstract: Five new wood-inhabiting fungi, Lyomyces albopulverulentus, L. yunnanensis, Xylodon daweishanensis, X. fissuratus, and X. puerensis spp. nov., are proposed based on a combination of morphological features and molecular evidence. Lyomyces albopulverulentus is characterized by brittle basidiomata, pruinose hymenophore with a white hymenial surface, a monomitic hyphal system with clamped generative hyphae, and ellipsoid basidiospores. Lyomyces yunnanensis is characterized by a grandinioid hymenial surface, the presence of capitate cystidia, and ellipsoid basidiospores. Xylodon daweishanensis is characterized by an odontioid hymenial surface, a monomitic hyphal system with clamped generative hyphae, and broad ellipsoid-to-subglobose basidiospores. Xylodon fissuratus is characterized by a cracking basidiomata with a grandinioid hymenial surface, and ellipsoid basidiospores. Xylodon puerensis is characterized by a poroid hymenophore with an angular or slightly daedaleoid configuration, and ellipsoid-to-broad-ellipsoid basidiospores. Sequences of ITS and nLSU rRNA markers of the studied samples were generated and phylogenetic analyses were performed with the maximum likelihood, maximum parsimony, and Bayesian inference methods. The phylogram based on the ITS+nLSU rDNA gene regions (Fig. 1) included six genera within the families Chaetoporellaceae, Hyphodontiaceae, Hymenochaetaceae, and Schizoporaceae (Hymenochaetales)—Fasciodontia, Hastodontia, Hyphodontia, Kneifiella, Lyomyces, and Xylodon—in which the five new species were grouped into genera Lyomyces and Xylodon. The phylogenetic tree inferred from the ITS sequences highlighted that Lyomyces albopulverulentus formed a monophyletic lineage and was then grouped closely with L. bambusinus, L. orientalis, and L. sambuci; additionally, L. yunnanensis was sister to L. niveus with strong supports. The topology, based on the ITS sequences, revealed that Xylodon daweishanensis was retrieved as a sister to X. hyphodontinus; X. fissuratus was grouped with the four taxa X. montanus, X. subclavatus, X. wenshanensis, and X. xinpingensis; and X. puerensis was clustered with X. flaviporus, X. ovisporus, X. subflaviporus, X. subtropicus, and X. taiwanianus.

HTML

XML

PDF

]]>
Research Article Tue, 14 Mar 2023 18:30:47 +0200
Segregation of the genus Parahypoxylon (Hypoxylaceae, Xylariales) from Hypoxylon by a polyphasic taxonomic approach https://mycokeys.pensoft.net/article/98125/ MycoKeys 95: 131-162

DOI: 10.3897/mycokeys.95.98125

Authors: Marjorie Cedeño-Sanchez, Esteban Charria-Girón, Christopher Lambert, J. Jennifer Luangsa-ard, Cony Decock, Raimo Franke, Mark Brönstrup, Marc Stadler

Abstract: During a mycological survey of the Democratic Republic of the Congo, a fungal specimen that morphologically resembled the American species Hypoxylon papillatum was encountered. A polyphasic approach including morphological and chemotaxonomic together with a multigene phylogenetic study (ITS, LSU, tub2, and rpb2) of Hypoxylon spp. and representatives of related genera revealed that this strain represents a new species of the Hypoxylaceae. However, the multi-locus phylogenetic inference indicated that the new fungus clustered with H. papillatum in a separate clade from the other species of Hypoxylon. Studies by ultrahigh performance liquid chromatography coupled to diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS) were carried out on the stromatal extracts. In particular, the MS/MS spectra of the major stromatal metabolites of these species indicated the production of hitherto unreported azaphilone pigments with a similar core scaffold to the cohaerin-type metabolites, which are exclusively found in the Hypoxylaceae. Based on these results, the new genus Parahypoxylon is introduced herein. Aside from P. papillatum, the genus also includes P. ruwenzoriense sp. nov., which clustered together with the type species within a basal clade of the Hypoxylaceae together with its sister genus Durotheca.

HTML

XML

PDF

]]>
Research Article Mon, 20 Feb 2023 14:05:41 +0200
Chaenothecopsis (Mycocaliciales, Ascomycota) from exudates of endemic New Zealand Podocarpaceae https://mycokeys.pensoft.net/article/97601/ MycoKeys 95: 101-129

DOI: 10.3897/mycokeys.95.97601

Authors: Christina Beimforde, Alexander R. Schmidt, Hanna Tuovila, Uwe Kaulfuss, Juliane Germer, William G. Lee, Jouko Rikkinen

Abstract: The order Mycocaliciales (Ascomycota) comprises fungal species with diverse, often highly specialized substrate ecologies. Particularly within the genus Chaenothecopsis, many species exclusively occur on fresh and solidified resins or other exudates of vascular plants. In New Zealand, the only previously known species growing on plant exudate is Chaenothecopsis schefflerae, found on several endemic angiosperms in the family Araliaceae. Here we describe three new species; Chaenothecopsis matai Rikkinen, Beimforde, Tuovila & A.R. Schmidt, C. nodosa Beimforde, Tuovila, Rikkinen & A.R. Schmidt, and C. novae-zelandiae Rikkinen, Beimforde, Tuovila & A.R. Schmidt, all growing on exudates of endemic New Zealand conifers of the Podocarpaceae family, particularly on Prumnopitys taxifolia. Phylogenetic analyses based on ribosomal DNA regions (ITS and LSU) grouped them into a distinct, monophyletic clade. This, as well as the restricted host range, suggests that all three taxa are endemic to New Zealand. Copious insect frass between the ascomata contain ascospores or show an early stage of ascomata development, indicating that the fungi are spread by insects. The three new species represent the first evidence of Chaenothecopsis from any Podocarpaceae species and the first from any gymnosperm exudates in New Zealand.

HTML

XML

PDF

]]>
Research Article Thu, 16 Feb 2023 17:36:21 +0200
Two new species of Astrothelium from Sud Yungas in Bolivia and the first discovery of vegetative propagules in the family Trypetheliaceae (lichen-forming Dothideomycetes, Ascomycota) https://mycokeys.pensoft.net/article/98986/ MycoKeys 95: 83-100

DOI: 10.3897/mycokeys.95.98986

Authors: Martin Kukwa, Pamela Rodriguez-Flakus, André Aptroot, Adam Flakus

Abstract: Two new species of Astrothelium are described from the Yungas forest in Bolivian Andes. Astrothelium chulumanense is characterised by pseudostromata concolorous with the thallus, perithecia immersed for the most part, with the upper portion elevated above the thallus and covered, except the tops, with orange pigment, apical and fused ostioles, the absence of lichexanthone (but thallus UV+ orange-yellow), clear hamathecium, 8-spored asci and amyloid, large, muriform ascospores with median septa. Astrothelium isidiatum is known only in a sterile state and produces isidia that develop in groups on areoles, but easily break off to reveal a medulla that resembles soralia. Both species, according to the two-locus phylogeny, belong to Astrothelium s.str. The production of isidia is reported from the genus Astrothelium and the family Trypetheliaceae for the first time.

HTML

XML

PDF

]]>
Research Article Wed, 8 Feb 2023 17:10:29 +0200
Additions to Thelebolales (Leotiomycetes, Ascomycota): Pseudogeomyces lindneri gen. et sp. nov. and Pseudogymnoascus campensis sp. nov. https://mycokeys.pensoft.net/article/97474/ MycoKeys 95: 47-60

DOI: 10.3897/mycokeys.95.97474

Authors: Zhi-Yuan Zhang, Yan-Feng Han, Wan-Hao Chen, Gang Tao

Abstract: Thelebolales are globally distributed fungi with diverse ecological characteristics. The classification of Thelebolales remains controversial to date and this study introduces two new taxa, based on morphological and phylogenetic analyses. The results of phylogenetic analyses indicated that the new taxa formed distinct lineages with strong support that were separated from the other members of Thelebolales. The new taxa described herein did not form sexual structures. The phylogenetic relationships of the new taxa and the morphological differences between these taxa and the other species under Thelebolales are also discussed.

HTML

XML

PDF

]]>
Research Article Mon, 6 Feb 2023 17:59:55 +0200
Morphological and phylogenetic analyses reveal two new species and a new record of Apiospora (Amphisphaeriales, Apiosporaceae) in China https://mycokeys.pensoft.net/article/96400/ MycoKeys 95: 27-45

DOI: 10.3897/mycokeys.95.96400

Authors: Rongyu Liu, Duhua Li, Zhaoxue Zhang, Shubin Liu, Xinye Liu, Yixin Wang, Heng Zhao, Xiaoyong Liu, Xiuguo Zhang, Jiwen Xia, Yujiao Wang

Abstract: The genus Apiospora includes endophytes, pathogens and saprobes, with a wide host range and geographic distribution. In this paper, six Apiospora strains isolated from diseased and healthy tissues of bamboo leaves from Hainan and Shandong provinces in China were classified using a multi-locus phylogeny based on a combined dataset of ITS, LSU, tef1 and tub2, in conjunction with morphological characters, host association and ecological distribution. Two new species, Apiospora dongyingensis and A. hainanensis, and a new record of A. pseudosinensis in China, are described based on their distinct phylogenetic relationships and morphological analyses. Illustrations and descriptions of the three taxa are provided, along with comparisons with closely related taxa in the genus.

HTML

XML

PDF

]]>
Research Article Fri, 27 Jan 2023 17:48:01 +0200
Phaeotubakia lithocarpicola gen. et sp. nov. (Tubakiaceae, Diaporthales) from leaf spots in China https://mycokeys.pensoft.net/article/98384/ MycoKeys 95: 15-25

DOI: 10.3897/mycokeys.95.98384

Authors: Ning Jiang, Ya-Quan Zhu, Han Xue, Chun-Gen Piao, Yong Li

Abstract: Tubakiaceae represents a distinct lineage of Diaporthales, including its type genus Tubakia and nine additional known genera. Tubakiaceous species are commonly known as endophytes in leaves and twigs of many tree species, but can also be plant pathogens causing conspicuous leaf symptoms. In the present study, isolates were obtained from diseased leaves of Lithocarpus glaber collected in Guangdong Province, China. The identification was conducted based on morphology and phylogeny of combined loci of 28S nrRNA gene (LSU), internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS) of the nrDNA operon, translation elongation factor 1-alpha (tef1) and beta tubulin (tub2). As a result, a distinct clade in Tubakiaceae was revealed named Phaeotubakia lithocarpicola gen. et sp. nov., which was distinguished from the other tubakiaceous taxa by its dark brown conidiogenous cells and conidia.

HTML

XML

PDF

]]>
Research Article Mon, 9 Jan 2023 10:01:28 +0200
Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern China https://mycokeys.pensoft.net/article/89425/ MycoKeys 94: 91-108

DOI: 10.3897/mycokeys.94.89425

Authors: Tao Sun, Weiqiu Zou, Quanying Dong, Ou Huang, Dexiang Tang, Hong Yu

Abstract: Ophiocordyceps contains the largest number of Cordyceps sensu lato, various species of which are of great medicinal value. In this study, a new entomopathogenic fungus, Ophiocordyceps nujiangensis, from Yunnan in southwestern China, was described using morphological, phylogenetic, and mitogenomic evidence, and its fungal community composition was identified. It was morphologically characterized by a solitary, woody, and dark brown stromata, smooth-walled and septate hyphae, solitary and gradually tapering conidiogenous cells with plenty of warty protrusions, and oval or fusiform conidia (6.4–11.2 × 3.7–6.4 µm) with mucinous sheath. The phylogenetic location of O. nujiangensis was determined based on the Bayesian inference (BI) and the maximum likelihood (ML) analyses by concatenating nrSSU, nrLSU, tef-1a, rpb1, and rpb2 datasets, and ten mitochondrial protein-coding genes (PCGs) datasets (atp6, atp9, cob, cox2, nad1, nad2, nad3, nad4, nad4L, and nad5). Phylogenetic analyses revealed that O. nujiangensis belonged to the Hirsutella sinensis subclade within the Hirsutella clade of Ophiocordyceps. And O. nujiangensis was phylogenetically clustered with O. karstii, O. liangshanensis, and O. sinensis. Simultaneously, five fungal phyla and 151 fungal genera were recognized in the analysis of the fungal community of O. nujiangensis. The fungal community composition differed from that of O. sinensis, and differences in the microbial community composition of closely related species might be appropriate as further evidence for taxonomy.

HTML

XML

PDF

]]>
Research Article Wed, 21 Dec 2022 11:30:43 +0200
Three new species of Nigrograna (Dothideomycetes, Pleosporales) associated with Arabica coffee from Yunnan Province, China https://mycokeys.pensoft.net/article/95751/ MycoKeys 94: 51-71

DOI: 10.3897/mycokeys.94.95751

Authors: Li Lu, Samantha C. Karunarathna, Dong-qin Dai, Ruvishika S. Jayawardena, Nakarin Suwannarach, Saowaluck Tibpromma

Abstract: Coffee is one of the most important cash crops in Yunnan Province, China. Yunnan is ranked as the biggest producer of high-quality coffee in China. During surveys of microfungi from coffee plantations in Yunnan, six fungal strains that resemble Nigrogranaceae were collected. Multi-gene analyses of a combined SSU-LSU-ITS-rpb2-tef1-α sequence data matrix were used to infer the phylogenetic position of the new species in Nigrograna while morphological characteristics were used to deduce the taxonomic position of the new species. Six fungal strains isolated from decaying branches of Coffea arabica represent three new saprobic species in Nigrograna. The three new species, N. asexualis, N. coffeae, and N. puerensis, are described with full (macro and micro characteristics) descriptions, illustrations, and a phylogenetic tree that shows the phylogenetic position of new taxa.

HTML

XML

PDF

]]>
Research Article Mon, 12 Dec 2022 10:40:56 +0200
New species and records of Neomassaria, Oxydothis and Roussoella (Pezizomycotina, Ascomycota) associated with palm and bamboo from China https://mycokeys.pensoft.net/article/89888/ MycoKeys 93: 165-191

DOI: 10.3897/mycokeys.93.89888

Authors: Hong Min Hu, Li Li Liu, Xu Zhang, Yan Lin, Xiang Chun Shen, Si Han Long, Ji Chuan Kang, Nalin N. Wijayawardene, Qi Rui Li, Qing De Long

Abstract: Several micro fungi were gathered from bamboo and palm in Guizhou Province, China. In morphology, these taxa resemble Neomassaria, Roussoella and Oxydothis. Multi-gene phylogenetic analyses based on combined ITS, LSU, SSU, rpb2 and tef1 loci confirmed that two are new geographical records for China, (viz. Roussoella siamensis, Neomassaria fabacearum), while two of them are new to science (viz. Oxydothis fortunei sp. nov. and Roussoella bambusarum sp. nov.). The stromata of Roussoella bambusarum are similar to those of R. thailandica, but its ascospores are larger. In addition, multi-gene phylogenetic analyses show that Oxydothis fortunei is closely related to O. inaequalis, but the J- ascus subapical ring as well as the ascospores of O. inaequalis are smaller. Morphological descriptions and illustrations of all species are provided.

HTML

XML

PDF

]]>
Research Article Thu, 10 Nov 2022 18:08:50 +0200
Note on the genus Nemania (Xylariaceae) – first records and a new species of the genus from Iran https://mycokeys.pensoft.net/article/94148/ MycoKeys 93: 81-105

DOI: 10.3897/mycokeys.93.94148

Authors: Mohammad Javad Pourmoghaddam, Christopher Lambert, Hermann Voglmayr, Seyed Akbar Khodaparast, Irmgard Krisai-Greilhuber, Marc Stadler

Abstract: In a survey of xylarialean fungi in northern Iran, some specimens attributable to the genus Nemania were collected, cultured and sequenced. Morphological evidence and phylogenetic analyses of a combined ITS, LSU, RPB2 and TUB2 gene dataset confirmed the presence of Nemania diffusa and N. serpens in Iran for the first time. Furthermore, the new species N. hyrcana, which shows similarities to N. subaenea and its putative synonym N. plumbea, but significantly differs from the latter in its DNA sequences, was encountered. All species are illustrated, described and discussed. In the phylogenetic analyses, for the first time, the overlooked ex-type ITS sequences of the neotype of the generic type, N. serpens and that of the holotype of N. prava, were added to a multi-gene matrix of Nemania. This revealed that the two accessions of N. serpens (HAST 235 and CBS 679.86), for which multigene data are available in GenBank, are misidentified, while the Iranian accession of N. serpens has an almost identical ITS sequence to the neotype, confirming its morphological species identification. The two previously accepted species of Euepixylon, E. udum and E. sphaeriostomum, are embedded within Nemania and are revealed as close relatives of N. serpens, supporting the inclusion of Euepixylon in Nemania.

HTML

XML

PDF

]]>
Research Article Fri, 7 Oct 2022 17:51:18 +0300
Multigene phylogeny and morphology reveal Ophiocordyceps hydrangea sp. nov. and Ophiocordyceps bidoupensis sp. nov. (Ophiocordycipitaceae) https://mycokeys.pensoft.net/article/86160/ MycoKeys 92: 109-130

DOI: 10.3897/mycokeys.92.86160

Authors: Weiqiu Zou, Dexiang Tang, Zhihong Xu, Ou Huang, Yuanbing Wang, Ngoc-Lan Tran, Hong Yu

Abstract: Ophiocordyceps species have a wide range of insect hosts, from solitary beetle larva to social insects. However, among the species of Ophiocordyceps, only a few attack cicada nymphs. These species are mainly clustered in the Ophiocordyceps sobolifera clade in Ophiocordyceps. A new entomopathogenic fungus parasitic on cicada nymphs, and another fungus parasitic on the larva of Coleoptera, are described in this study. The two new species viz. Ophiocordyceps hydrangea and Ophiocordyceps bidoupensis were introduced based on morphology and multigene phylogenetic evidence. The phylogenetic framework of Ophiocordyceps was reconstructed using a multigene (nrSSU, nr LSU, tef-1α, rpb1, and rpb2) dataset. The phylogenetic analyses results showed that O. hydrangea and O. bidoupensis were statistically well-supported in the O. sobolifera clade, forming two separate subclades from other species of Ophiocordyceps. The distinctiveness of these two new species was strongly supported by both molecular phylogeny and morphology.

HTML

XML

PDF

]]>
Research Article Tue, 30 Aug 2022 09:47:25 +0300
Comprehensive treatise of Hevansia and three new genera Jenniferia, Parahevansia and Polystromomyces on spiders in Cordycipitaceae from Thailand https://mycokeys.pensoft.net/article/83091/ MycoKeys 91: 113-149

DOI: 10.3897/mycokeys.91.83091

Authors: Suchada Mongkolsamrit, Wasana Noisripoom, Kanoksri Tasanathai, Noppol Kobmoo, Donnaya Thanakitpipattana, Artit Khonsanit, Booppa Petcharad, Baramee Sakolrak, Winanda Himaman

Abstract: Collections of pathogenic fungi found on spiders from Thailand were selected for a detailed taxonomic study. Morphological comparison and phylogenetic analyses of the combined ITS, LSU, tef1, rpb1 and rpb2 sequence data indicated that these specimens formed new independent lineages within the Cordycipitaceae, containing two new genera occurring on spiders, i.e. Jenniferia gen. nov. and Polystromomyces gen. nov. Two new species in Jenniferia, J. griseocinerea sp. nov. and J. thomisidarum sp. nov., are described. Two strains, NHJ 03510 and BCC 2191, initially named as Akanthomyces cinereus (Hevansia cinerea), were shown to be part of Jenniferia. By including sequences of putative Hevansia species from GenBank, we also revealed Parahevansia as a new genus with the ex-type strain NHJ 666.01 of Pa. koratensis, accommodating specimens previously named as Akanthomyces koratensis (Hevansia koratensis). One species of Polystromomyces, Po. araneae sp. nov., is described. We established an asexual-sexual morph connection for Hevansia novoguineensis (Cordycipitaceae) with ex-type CBS 610.80 and proposed a new species, H. minuta sp. nov. Based on characteristics of the sexual morph, Hevansia and Polystromomyces share phenotypic traits by producing stipitate ascoma with fertile terminal heads; however, they differ in the shape and colour of the stipes. Meanwhile, Jenniferia produces non-stipitate ascoma with aggregated superficial perithecia forming a cushion. A new morphology of ascospores in Jenniferia is described, illustrated and compared with other species in Cordycipitaceae.

HTML

XML

PDF

]]>
Research Article Tue, 26 Jul 2022 10:49:18 +0300
Four new species of Diaporthe (Diaporthaceae, Diaporthales) from forest plants in China https://mycokeys.pensoft.net/article/84970/ MycoKeys 91: 25-47

DOI: 10.3897/mycokeys.91.84970

Authors: Lingxue Cao, Dun Luo, Wu Lin, Qin Yang, Xiaojun Deng

Abstract: Species of Diaporthe inhabit a wide range of plant hosts as plant pathogens, endophytes and saprobes. During trips to collect forest pathogens in Beijing, Jiangxi, Shaanxi and Zhejiang Provinces in China, 16 isolates of Diaporthe were obtained from branch cankers and leaf spots. These isolates were studied by applying a polyphasic approach including morphological, cultural data, and phylogenetic analyses of the nuclear ribosomal internal transcribed spacer (ITS), calmodulin (cal), histone H3 (his3), partial translation elongation factor-1α (tef-1α) and β-tubulin (tub2) loci. Results revealed four new taxa, D. celticola, D. meliae, D. quercicola, D. rhodomyrti spp. nov. and two known species, D. eres and D. multiguttulata.

HTML

XML

PDF

]]>
Research Article Wed, 6 Jul 2022 11:12:42 +0300
Morphological and phylogenetic analyses reveal two new species and a new record of Phyllosticta (Botryosphaeriales, Phyllostictaceae) from Hainan, China https://mycokeys.pensoft.net/article/84803/ MycoKeys 91: 1-23

DOI: 10.3897/mycokeys.91.84803

Authors: Zhaoxue Zhang, Xiaoyong Liu, Xiuguo Zhang, Zhe Meng

Abstract: The fungal genus Phyllosticta has been reported from all around the world and accommodates numerous pathogenic and endophytic species isolated from a wide range of plant hosts. Based on multilocus phylogenies from a combined dataset of genes encoding internal transcribed spacer (ITS), large subunit of ribosomal RNA (LSU rDNA), translation elongation factor 1 alpha (TEF1α), actin (ACT) and glycerol-3-phosphate dehydrogenase (GPDH), in conjunction with morphological characteristics, we describe two new species P. oblongifoliae sp. nov. and P. pterospermi sp. nov., as well as a new Chinese record P. capitalensis. Their similarity and dissimilarity to morphologically-allied and phylogenetically-related species are also annotated and discussed.

HTML

XML

PDF

]]>
Research Article Mon, 4 Jul 2022 11:33:46 +0300
Not (only) poison pies – Hebeloma (Agaricales, Hymenogastraceae) in Mexico https://mycokeys.pensoft.net/article/85267/ MycoKeys 90: 163-202

DOI: 10.3897/mycokeys.90.85267

Authors: Ursula Eberhardt, Alejandro Kong, Adriana Montoya, Nicole Schütz, Peter Bartlett, Henry J. Beker

Abstract: The species of Hebeloma have been little studied in Mexico, but have received attention as edibles and in trials to enhance production of edible fungi and tree growth through inoculation of seedlings with ectomycorrhizal fungi. Here we describe three new species of Hebeloma that are currently known only from Mexico. These species belong to separate sections of the genus: H. ambustiterranum is a member of H. sect. Hebeloma, H. cohaerens belongs to H. sect. Theobromina, while H. magnicystidiatum belongs to H. sect. Denudata. All three species were collected from subtropical pine-oak woodland; all records of H. cohaerens came from altitudes above 2500 m. Hebeloma ambustiterranum is commonly sold in the local markets of Tlaxcala as a prized edible mushroom. An additional nine species are reported from Mexico, of which eight are new records for the country: H. aanenii, H. eburneum, H. excedens, H. ingratum, H. neurophyllum, H. sordidulum, H. subaustrale and H. velutipes. First modern descriptions of H. neurophyllum and H. subaustrale, originally described from the USA, are given here.

HTML

XML

PDF

]]>
Research Article Thu, 30 Jun 2022 00:05:50 +0300
Taxonomic studies of bluish Mycena (Mycenaceae, Agaricales) with two new species from northern China https://mycokeys.pensoft.net/article/78880/ MycoKeys 90: 119-145

DOI: 10.3897/mycokeys.90.78880

Authors: Qin Na, Zewei Liu, Hui Zeng, Binrong Ke, Zhizhong Song, Xianhao Cheng, Yupeng Ge

Abstract: Bluish Mycena are rare, but constitute a taxonomically complex group. A total of eight bluish species in four sections have previously been reported from North America, Europe, Oceania and Asia. Two species with a blue pileus, collected in China during our taxonomic study of Mycena s.l., are described here as new to science: Mycena caeruleogrisea sp. nov. and M. caeruleomarginata sp. nov. Detailed descriptions, line drawings and a morphological comparison with closely-related species, especially herbarium specimens of M. subcaerulea from the USA, are provided. The results of Bayesian Inference and Maximum Likelihood phylogenetic analyses of a dataset of 96 nuclear rDNA ITS and 20 nLSU sequences of 43 Mycena species are also presented. The morphological data and the results of the phylogenetic analyses support the introduction of M. caeruleogrisea and M. caeruleomarginata as new species. A taxonomic key to bluish Mycena species of sections Amictae, Cyanocephalae, Sacchariferae and Viscipelles is provided.

HTML

XML

PDF

]]>
Research Article Fri, 17 Jun 2022 12:31:30 +0300
Two new species of Phylloporia (Hymenochaetales) from the Neotropics https://mycokeys.pensoft.net/article/84767/ MycoKeys 90: 71-83

DOI: 10.3897/mycokeys.90.84767

Authors: Meng Zhou, Fang Wu, Yu-Cheng Dai, Josef Vlasák

Abstract: Two new species of Phylloporia, P. crystallina and P. sumacoensis, are described based on 28S ribosomal RNA phylogeny, morphology, host, and geographic distribution. Phylloporia crystallina is characterized by pileate, perennial basidiomata with a duplex context, small pores 9–10 per mm, a monomitic hyphal system, absence of cystidia and cystidioles, presence of large rhomboid crystals in tube trama, broadly ellipsoid to subglobose basidiospores measuring 2.8–3 × 2–2.3 μm, and growth on angiosperm stump. Phylloporia sumacoensis is characterized by pileate, perennial basidiomata with a duplex context, very small pores 10–12 per mm, a monomitic hyphal system, hyphae at dissepiment edges bearing fine crystals, presence of cystidioles, broadly ellipsoid to subglobose basidiospores measuring 3–3.7 × 2.1–2.8 μm, and growth on living liana.

HTML

XML

PDF

]]>
Research Article Tue, 7 Jun 2022 17:25:14 +0300
Taxonomy and molecular phylogeny of Trametopsis (Polyporales, Basidiomycota) with descriptions of two new species https://mycokeys.pensoft.net/article/84717/ MycoKeys 90: 31-51

DOI: 10.3897/mycokeys.90.84717

Authors: Shun Liu, Yi-Fei Sun, Yan Wang, Tai-Min Xu, Chang-Ge Song, Yuan-Yuan Chen, Bao-Kai Cui

Abstract: Trametopsis is a worldwide genus belonging to Irpicaceae in the phlebioid clade, which can cause a white decay of wood. Previously, only three species were ascribed to the genus. In this study, we performed a morphological and phylogenetic study of Trametopsis. Molecular phylogenetic analyses of multiple loci included the internal transcribed spacer (ITS) regions, the large subunit nuclear ribosomal RNA gene (nLSU), the largest subunit of RNA polymerase II (RPB1), the second largest subunit of RNA polymerase II (RPB2) and the translation elongation factor 1-α gene (TEF1). Phylogenetic trees were inferred from the combined datasets of ITS+nLSU sequences and ITS+nLSU+RPB1+RPB2+TEF1 sequences by using maximum parsimony, maximum likelihood and Bayesian inference analyses. Combined with molecular data, morphological characters and ecological traits, two new species of Trametopsis are discovered. Trametopsis abieticola is characterised by its pileate, solitary or imbricate basidiomata, buff to buff-yellow pileal surface when fresh, becoming pinkish buff to clay-buff when dry, cream to buff pore surface when fresh, becoming pinkish buff to greyish brown upon drying, round to angular and large pores (0.5–1 per mm), cylindrical basidiospores (5.8–7.2 × 1.9–2.6 μm), distributed in the high altitude of mountains and grows on Abies sp. Trametopsis tasmanica is characterised by its resupinate basidiomata, cream to pinkish-buff pore surface when fresh, becoming honey-yellow to snuff brown upon drying, cylindrical basidiospores (5.2–6.3 × 1.8–2.2 μm), and by growing on Eucalyptus sp. Detailed descriptions and illustrations of the two novel species are provided.

HTML

XML

PDF

]]>
Research Article Tue, 31 May 2022 18:44:36 +0300
Dendrocorticiopsis orientalis gen. et sp. nov. of the Punctulariaceae (Corticiales, Basidiomycota) revealed by molecular data https://mycokeys.pensoft.net/article/84562/ MycoKeys 90: 19-30

DOI: 10.3897/mycokeys.90.84562

Authors: Chia-Ling Wei, Che-Chih Chen, Shuang-Hui He, Sheng-Hua Wu

Abstract: Dendrocorticiopsis orientalis is presented in this study as a new genus and new species based on morphological and phylogenetic evidence. This new taxon is characterized by resupinate, smooth and membranaceous basidiomata, monomitic hyphal system with clamps, colorless dendrohyphidia, variable presence of cystidia, and ellipsoid to ovoid basidiospores measuring 5–7 × 3.2–5.2 μm. The phylogenetic analyses based on the ITS1-5.8S-ITS2 (ITS) + nuclear 28S rDNA (28S) dataset of Corticiales indicated that the new taxon is nested in Punctulariaceae, separated from other genera with strong support values. Descriptions, specimen photo, and illustrations of the new taxon are provided in this study. A morphological comparison of the four genera of Punctulariaceae is given.

HTML

XML

PDF

]]>
Research Article Tue, 31 May 2022 09:45:56 +0300
Two new species in Capillidium (Ancylistaceae, Entomophthorales) from China, with a proposal for a new combination https://mycokeys.pensoft.net/article/79537/ MycoKeys 89: 139-153

DOI: 10.3897/mycokeys.89.79537

Authors: Yong Nie, Heng Zhao, ZiMin Wang, ZhengYu Zhou, XiaoYong Liu, Bo Huang

Abstract: A taxonomic revision of Conidiobolus s.l. (Ancylistaceae, Entomophthorales) delimited all members that form capilliconidia into the genus Capillidium. In this study, we report two new species of Capillidium that were isolated in China. Capillidium macrocapilliconidium sp. nov. is characterised by large capilliconidia. Capillidium jiangsuense sp. nov. is differentiated by large capilliconidia and long, slender secondary conidiophores. Phylogenetic analyses were performed using sequences from the nuclear large subunit of rDNA (nucLSU), the mitochondrial small subunit of rDNA (mtSSU) and elongation-factor-like (EFL). The analyses revealed sister relationships between Ca. macrocapilliconidium sp. nov. and Ca. globuliferus / Ca. pumilum and between Ca. jiangsuense sp. nov. and Ca. denaeosporum. Additionally, a new combination of Ca. rugosum (Drechsler) B. Huang & Y. Nie comb. nov. is proposed herein. An identification key is provided for the ten accepted Capillidium species.

HTML

XML

PDF

]]>
Research Article Fri, 29 Apr 2022 10:29:45 +0300
Morphological and phylogenetic analyses reveal two new species of Sporocadaceae from Hainan, China https://mycokeys.pensoft.net/article/82229/ MycoKeys 88: 171-192

DOI: 10.3897/mycokeys.88.82229

Authors: Zhaoxue Zhang, Rongyu Liu, Shubin Liu, Taichang Mu, Xiuguo Zhang, Jiwen Xia

Abstract: Species of Sporocadaceae have often been reported as plant pathogens, endophytes or saprophytes and are commonly isolated from a wide range of plant hosts. The isolated fungi were studied through a complete examination, based on multilocus phylogenies from combined datasets of ITS/tub2/tef1, in conjunction with morphological characteristics. Nine strains were isolated from Ficus microcarpa, Ilex chinensis and Schima superba in China which represented four species, viz., Monochaetia schimae sp. nov., Neopestalotiopsis haikouensis sp. nov., Neopestalotiopsis piceana and Pestalotiopsis licualicola. Neopestalotiopsis piceana was a new country record for China and first host record from Ficus macrocarpa. Pestalotiopsis licualicola was first report from Ilex chinensis in China.

HTML

XML

PDF

]]>
Research Article Thu, 14 Apr 2022 18:17:15 +0300
Three new species of Candolleomyces (Agaricomycetes, Agaricales, Psathyrellaceae) from the Yanshan Mountains in China https://mycokeys.pensoft.net/article/81437/ MycoKeys 88: 109-121

DOI: 10.3897/mycokeys.88.81437

Authors: Hao Zhou, GuiQiang Cheng, XiMei Sun, RuiYi Cheng, HongLiang Zhang, YanMin Dong, ChengLin Hou

Abstract: Three new species, Candolleomyces incanus, C. subcandolleanus and C. yanshanensis, were found and described from Yanshan Mountains in China. The identification is based on morphological observation combined with phylogenetic analysis of ITS-LSU-Tef1α-TUB2. This study enriched the species diversity of Candolleomyces in Yanshan Mountains and provided important data support for the systematic study of Candolleomyces in the future.

HTML

XML

PDF

]]>
Research Article Wed, 13 Apr 2022 14:54:13 +0300
Hemiaustroboletus, a new genus in the subfamily Austroboletoideae (Boletaceae, Boletales) https://mycokeys.pensoft.net/article/73951/ MycoKeys 88: 55-78

DOI: 10.3897/mycokeys.88.73951

Authors: Olivia Ayala-Vásquez, Jesús García-Jiménez, Elvira Aguirre-Acosta, Rigoberto Castro-Rivera, Rodolfo Enrique Ángeles-Argáiz, Ángel Emmanuel Saldivar, Roberto Garibay-Orijel

Abstract: The present study describes Hemiaustroboletus gen. nov. in the subfamily Austroboletoideae (Boletaceae). Hemiaustroboletus is supported by morphological and molecular data using LSU and RPB2 regions. Additionally, its geographic distribution and intraspecific variation were inferred using ITS sequences. The genus is characterised by pileate-stipitate basidiomata; purple, brown, reddish-brown, orange-brown to dark brown vinaceous pileus; whitish or lilac to vinaceous context and a subclavate stipe. Microscopically, it is characterised by ornamented, slightly verrucose, cracked to perforated brown basidiospores. Two species are described within the genus, Hemiaustroboletus vinaceobrunneus sp. nov. and H. vinaceus sp. nov. Hemiaustroboletus vinaceus sp. nov. is morphologically similar to Austroboletus gracilis, which suggests they may have been confused in the past. This study presents the phylogenetic placement, microscopic structures, detailed morphological descriptions and illustrations of both new species.

HTML

XML

PDF

]]>
Research Article Wed, 30 Mar 2022 09:53:17 +0300
Three novel species of Distoseptispora (Distoseptisporaceae) isolated from bamboo in Jiangxi Province, China https://mycokeys.pensoft.net/article/79346/ MycoKeys 88: 35-54

DOI: 10.3897/mycokeys.88.79346

Authors: Zhi-Jun Zhai, Jun-Qing Yan, Wei-Wu Li, Yang Gao, Hai-Jing Hu, Jian-Ping Zhou, Hai-Yan Song, Dian-Ming Hu

Abstract: Decaying bamboo in freshwater is a unique eco-environment for fungi. Three new Distoseptispora (Distoseptisporaceae) species, D. meilingensis, D. yongxiuensis and D. yunjushanensis from submerged decaying bamboo culms in Jiangxi Province, China, were discovered, based on phylogenetic analyses and morphological characters. The combined data of ITS-LSU-SSU-Tef1 sequences were used to infer the phylogenetic relationship between D. meilingensis, D. yongxiuensis, D. yunjushanensis and related species. Both molecular analyses and morphological data supported D. meilingensis, D. yongxiuensis and D. yunjushanensis as three independent taxa.

HTML

XML

PDF

]]>
Research Article Tue, 22 Mar 2022 10:14:01 +0200
Revision of Immersaria and a new lecanorine genus in Lecideaceae (lichenised Ascomycota, Lecanoromycetes) https://mycokeys.pensoft.net/article/72614/ MycoKeys 87: 99-132

DOI: 10.3897/mycokeys.87.72614

Authors: Cong-Miao Xie, Li-Song Wang, Zun-Tian Zhao, Yan-Yun Zhang, Xin-Yu Wang, Lu-Lu Zhang

Abstract: The species Immersaria cupreoatra has been included in Bellemerea. This caused us to reconsider the relationships between Bellemerea and the lecanorine species of Immersaria and to question the monophyly of Immersaria. Amongst 25 genera of the family Lecideaceae, most have lecideine apothecia, the exceptions being Bellemerea and Koerberiella, which have lecanorine apothecia. According to previous classifications, Immersaria included species with both lecanorine and lecideine apothecia. A five-loci phylogenetic tree (nrITS, nrLSU, RPB1, RPB2, and mtSSU) for Lecideaceae showed that Immersaria was split into two clades: firstly, all the lecideine apotheciate species and secondly, all the lecanorine apotheciate species. The latter clade was closely related to the remaining lecanorine apotheciate genera: Bellemerea and Koerberiella. Therefore, the genus concept of Immersaria is revised accordingly and a new lecanorine genus Lecaimmeria is proposed. Furthermore, four new species for Immersaria and seven new species and three new combinations for the new genus Lecaimmeria are proposed. Keys to Immersaria and the new genus Lecaimmeria are provided.

HTML

XML

PDF

]]>
Research Article Tue, 15 Feb 2022 11:09:19 +0200
Diversity of Fusarium associated banana wilt in northern Viet Nam https://mycokeys.pensoft.net/article/72941/ MycoKeys 87: 53-76

DOI: 10.3897/mycokeys.87.72941

Authors: Loan Le Thi, Arne Mertens, Dang Toan Vu, Tuong Dang Vu, Pham Le Anh Minh, Huy Nguyen Duc, Sander de Backer, Rony Swennen, Filip Vandelook, Bart Panis, Mario Amalfi, Cony Decock, Sofia I. F. Gomes, Vincent S. F. T. Merckx, Steven B. Janssens

Abstract: Fusarium is one of the most important fungal genera of plant pathogens that affect the cultivation of a wide range of crops. Agricultural losses caused by Fusarium oxysporum f. sp. cubense (Foc) directly affect the income, subsistence, and nourishment of thousands of farmers worldwide. For Viet Nam, predictions on the impact of Foc for the future are dramatic, with an estimated loss in the banana production area of 8% within the next five years and up to 71% within the next 25 years. In the current study, we applied a combined morphological-molecular approach to assess the taxonomic identity and phylogenetic position of the different Foc isolates collected in northern Viet Nam. In addition, we aimed to estimate the proportion of the different Fusarium races infecting bananas in northern Viet Nam. The morphology of the isolates was investigated by growing the collected Fusarium isolates on four distinct nutritious media (PDA, SNA, CLA, and OMA). Molecular phylogenetic relationships were inferred by sequencing partial rpb1, rpb2, and tef1a genes and adding the obtained sequences into a phylogenetic framework. Molecular characterization shows that c. 74% of the Fusarium isolates obtained from infected banana pseudostem tissue belong to F. tardichlamydosporum. Compared to F. tardichlamydosporum, F. odoratissimum accounts for c.10% of the Fusarium wilt in northern Viet Nam, demonstrating that Foc TR4 is not yet a dominant strain in the region. Fusarium cugenangense – considered to cause Race 2 infections among bananas – is only found in c. 10% of the tissue material that was obtained from infected Vietnamese bananas. Additionally, one of the isolates cultured from diseased bananas was phylogenetically not positioned within the F. oxysporum species complex (FOSC), but in contrast, fell within the Fusarium fujikuroi species complex (FFSC). As a result, a possible new pathogen for bananas may have been found. Besides being present on several ABB ‘Tay banana’, F. tardichlamydosporum was also derived from infected tissue of a wild Musa lutea, showing the importance of wild bananas as a possible sink for Foc.

HTML

XML

PDF

]]>
Research Article Thu, 10 Feb 2022 16:07:41 +0200
The curse of the uncultured fungus https://mycokeys.pensoft.net/article/76053/ MycoKeys 86: 177-194

DOI: 10.3897/mycokeys.86.76053

Authors: Kessy Abarenkov, Erik Kristiansson, Martin Ryberg, Sandra Nogal-Prata, Daniela Gómez-Martínez, Katrin Stüer-Patowsky, Tobias Jansson, Sergei Põlme, Masoomeh Ghobad-Nejhad, Natàlia Corcoll, Ruud Scharn, Marisol Sánchez-García, Maryia Khomich, Christian Wurzbacher, R. Henrik Nilsson

Abstract: The international DNA sequence databases abound in fungal sequences not annotated beyond the kingdom level, typically bearing names such as “uncultured fungus”. These sequences beget low-resolution mycological results and invite further deposition of similarly poorly annotated entries. What do these sequences represent? This study uses a 767,918-sequence corpus of public full-length fungal ITS sequences to estimate what proportion of the 95,055 “uncultured fungus” sequences that represent truly unidentifiable fungal taxa – and what proportion of them that would have been straightforward to annotate to some more meaningful taxonomic level at the time of sequence deposition. Our results suggest that more than 70% of these sequences would have been trivial to identify to at least the order/family level at the time of sequence deposition, hinting that factors other than poor availability of relevant reference sequences explain the low-resolution names. We speculate that researchers’ perceived lack of time and lack of insight into the ramifications of this problem are the main explanations for the low-resolution names. We were surprised to find that more than a fifth of these sequences seem to have been deposited by mycologists rather than researchers unfamiliar with the consequences of poorly annotated fungal sequences in molecular repositories. The proportion of these needlessly poorly annotated sequences does not decline over time, suggesting that this problem must not be left unchecked.

HTML

XML

PDF

]]>
Research Article Wed, 2 Feb 2022 10:49:31 +0200
Thyridium revised: Synonymisation of Phialemoniopsis under Thyridium and establishment of a new order, Thyridiales https://mycokeys.pensoft.net/article/78989/ MycoKeys 86: 147-176

DOI: 10.3897/mycokeys.86.78989

Authors: Ryosuke Sugita, Kazuaki Tanaka

Abstract: The genus Thyridium, previously known as a saprobic or hemibiotrophic ascomycete on various plants, was revised taxonomically and phylogenetically. Sequences of the following six regions, that is, the nuclear ribosomal internal transcribed spacer (ITS) region, the large subunit (LSU) of rDNA, the second largest RNA polymerase II subunit (rpb2) gene, translation elongation factor 1-alpha (tef1) gene, the actin (act) gene, and the beta-tubulin (tub2) gene, were generated for molecular phylogenetic analyses of species of this genus. Phialemoniopsis, a genus encompassing medically important species, is synonymised with Thyridium based on molecular evidence and morphological similarities in their asexual characters. The generic concept for Thyridium is expanded to include species possessing both coelomycetous and hyphomycetous complex asexual morphs. In addition to type species of Thyridium, T. vestitum, nine species were accepted in Thyridium upon morphological comparison and molecular phylogenetic analyses in this study. All seven species of Phialemoniopsis were treated as members of the genus Thyridium and new combinations were proposed. A bambusicolous fungus, Pleospora punctulata, was transferred to Thyridium, and an epitype is designated for this species. A new species, T. flavostromatum, was described from Phyllostachys pubescens. The family Phialemoniopsidaceae, proposed as a familial placement for Phialemoniopsis, was regarded as a synonym of Thyridiaceae. A new order, Thyridiales, was established to accommodate Thyridiaceae; it forms a well-supported, monophyletic clade in Sordariomycetes.

HTML

XML

PDF

]]>
Research Article Tue, 1 Feb 2022 10:14:43 +0200
Redelimitation of Heteroradulum (Auriculariales, Basidiomycota) with H. australiense sp. nov. https://mycokeys.pensoft.net/article/76425/ MycoKeys 86: 87-101

DOI: 10.3897/mycokeys.86.76425

Authors: Qian-Zhu Li, Shi-Liang Liu, Xue-Wei Wang, Tom W. May, Li-Wei Zhou

Abstract: Auriculariales accommodates species with diverse basidiomes and hymenophores. From morphological and phylogenetic perspectives, we perform a taxonomic study on Heteroradulum, a recently validated genus within the Auriculariales. The genus Grammatus is merged into Heteroradulum, and thus its generic type G. labyrinthinus is combined with Heteroradulum and G. semis is reaccepted as a member of Heteroradulum. Heteroradulum australiense is newly described on the basis of three Australian specimens. Heteroradulum yunnanense is excluded from this genus and its taxonomic position at the generic level is considered uncertain. Accordingly, the circumscription of Heteroradulum is re-delimited and the concept of this genus is adjusted by including irpicoid to poroid hymenophores and a hyphal system with clamp connections or simple septa. A key to all nine accepted species of Heteroradulum is presented.

HTML

XML

PDF

]]>
Research Article Wed, 19 Jan 2022 16:51:08 +0200
Taxonomy and phylogeny of the novel rhytidhysteron-like collections in the Greater Mekong Subregion https://mycokeys.pensoft.net/article/70668/ MycoKeys 86: 65-85

DOI: 10.3897/mycokeys.86.70668

Authors: Guang-Cong Ren, Dhanushka N. Wanasinghe, Rajesh Jeewon, Jutamart Monkai, Peter E. Mortimer, Kevin D. Hyde, Jian-Chu Xu, Heng Gui

Abstract: During our survey into the diversity of woody litter fungi across the Greater Mekong Subregion, three rhytidhysteron-like taxa were collected from dead woody twigs in China and Thailand. These were further investigated based on morphological observations and multi-gene phylogenetic analyses of a combined DNA data matrix containing SSU, LSU, ITS, and tef1-α sequence data. A new species of Rhytidhysteron, R. xiaokongense sp. nov. is introduced with its asexual morph, and it is characterized by semi-immersed, subglobose to ampulliform conidiomata, dark brown, oblong to ellipsoidal, 1-septate, conidia, which are granular in appearance when mature. In addition to the new species, two new records from Thailand are reported viz. Rhytidhysteron tectonae on woody litter of Betula sp. (Betulaceae) and Fabaceae sp. and Rhytidhysteron neorufulum on woody litter of Tectona grandis (Lamiaceae). Morphological descriptions, illustrations, taxonomic notes and phylogenetic analyses are provided for all entries.

HTML

XML

PDF

]]>
Research Article Wed, 12 Jan 2022 15:14:34 +0200
 Azygosporus gen. nov., a synapmorphic clade in the family Ancylistaceae https://mycokeys.pensoft.net/article/73405/ MycoKeys 85: 161-172

DOI: 10.3897/mycokeys.85.73405

Authors: Yue Cai, Yong Nie, Heng Zhao, ZiMin Wang, ZhengYu Zhou, XiaoYong Liu, Bo Huang

Abstract: The fungal genus Conidiobolus sensu lato was delimited into four genera based on morphology and phylogeny. However, the taxonomic placement of C. parvus has not been determined until now. Here, we show that C. parvus belongs to a distinct lineage based on mitochondrial (mtSSU) and nuclear (TEF1 and nrLSU) phylogenetic analyses. Phylogenetic analyses further revealed a new species as sister to C. parvus. We identified a synapomorphy uniting these lineages (azygospore production) that was not observed in other allied genera of the family Ancylistaceae, and erected a new genus Azygosporus gen. nov. for this monophyletic group, with a new combination, A. parvus comb. nov. as the type species. Within Azygosporus, the novel species A. macropapillatus sp. nov. was introduced from China based on morphological characteristics and molecular evidence, which is characterized by its prominent basal papilla, in comparison to other closely related species, measuring 7.5–10.0×5.0–10.0 µm. Our study resolved the phylogenetic placement of C. parvus and improved the taxonomic system of the Ancylistaceae family.

HTML

XML

PDF

]]>
Research Article Fri, 31 Dec 2021 12:03:34 +0200
Infraspecific variation of some brown Parmeliae (in Poland) – a comparison of ITS rDNA and non-molecular characters https://mycokeys.pensoft.net/article/70552/ MycoKeys 85: 127-160

DOI: 10.3897/mycokeys.85.70552

Authors: Katarzyna Szczepańska, Beata Guzow-Krzemińska, Jacek Urbaniak

Abstract: Infraspecific variation of the ITS rDNA region of some brown Parmeliae occurring in Poland is studied and compared with non-molecular characters. Haplotype networks are used to illustrate the variability within the species. Both newly-produced sequences from Central Europe and from all over the world, downloaded from the GenBank, are used. The number of haplotypes found for each taxon ranged from five in Melanelia stygia to 12 in Melanelia hepatizon and Montanelia disjuncta; however, their numbers correlate with the number of specimens tested. New haplotypes for Melanelia agnata, M. hepatizon and Cetraria commixta are found. Based on our 169-sample dataset, we could not infer any geographical correlation, either locally or world-wide. Many of the analysed haplotypes were widely distributed and the same haplotype was often shared between temperate and polar populations. A comparison of molecular, morphological, anatomical and chemical characters also shows no correlation.

HTML

XML

PDF

]]>
Research Article Wed, 22 Dec 2021 14:13:04 +0200
Morphological and molecular phylogenetic analyses reveal three species of Colletotrichum in Shandong province, China https://mycokeys.pensoft.net/article/75944/ MycoKeys 85: 57-71

DOI: 10.3897/mycokeys.85.75944

Authors: Taichang Mu, Zhaoxue Zhang, Rongyu Liu, Shubin Liu, Zhuang Li, Xiuguo Zhang, Jiwen Xia

Abstract: Colletotrichum has numerous host range and distribution. Its species are important plant pathogens, endophytes and saprobes. Colletotrichum can cause regular or irregular depressions and necrotic lesions in the epidermal tissues of plants. During this research Colletotrichum specimens were collected from Mengyin County, Shandong Province, China. A multi-locus phylogenetic analysis of ITS, GAPDH, CHS-1, ACT, TUB2, CAL and GS sequence data combined with morphology, revealed a new species and two known species, viz. C. mengyinense sp. nov., C. gloeosporioides and C. pandanicola, belonging to the C. gloeosporioides species complex. The new species is described and illustrated in this paper and compared with taxa in the C. gloeosporioides species complex.

HTML

XML

PDF

]]>
Research Article Wed, 8 Dec 2021 11:37:18 +0200
Two new asexual genera and six new asexual species in the family Microthyriaceae (Dothideomycetes, Ascomycota) from China https://mycokeys.pensoft.net/article/70829/ MycoKeys 85: 1-30

DOI: 10.3897/mycokeys.85.70829

Authors: Min Qiao, Hua Zheng, Ji-Shu Guo, Rafael F. Castañeda-Ruiz, Jian-Ping Xu, Jie Peng, Ke-Qin Zhang, Ze-Fen Yu

Abstract: The family Microthyriaceae is represented by relatively few mycelial cultures and DNA sequences; as a result, the taxonomy and classification of this group of organisms remain poorly understood. During the investigation of the diversity of aquatic hyphomycetes from southern China, several isolates were collected. These isolates were cultured and sequenced and a BLAST search of its LSU sequences against data in GenBank revealed that the closest related taxa are in the genus Microthyrium. Phylogenetic analyses, based on the combined sequence data from the internal transcribed spacers (ITS) and the large subunit (LSU), revealed that these isolates represent eight new taxa in Microthyriaceae, including two new genera, Antidactylaria gen. nov. and Isthmomyces gen. nov. and six new species, Antidactylaria minifimbriata sp. nov., Isthmomyces oxysporus sp. nov., I. dissimilis sp. nov., I. macrosporus sp. nov., Triscelophorus anisopterioideus sp. nov. and T. sinensis sp. nov. These new taxa are described, illustrated for their morphologies and compared with similar taxa. In addition, two new combinations are proposed in this family.

HTML

XML

PDF

]]>
Research Article Mon, 29 Nov 2021 15:45:41 +0200
Morphological and phylogenetic analyses reveal a new genus and two new species of Tubakiaceae from China https://mycokeys.pensoft.net/article/73940/ MycoKeys 84: 185-201

DOI: 10.3897/mycokeys.84.73940

Authors: Zhaoxue Zhang, Taichang Mu, Shubin Liu, Rongyu Liu, Xiuguo Zhang, Jiwen Xia

Abstract: Species of Tubakiaceae have often been reported as plant pathogens or endophytes, commonly isolated from a wide range of plant hosts. The isolated fungi were studied through a complete examination, based on multilocus phylogenies from combined datasets of ITS/LSU/rpb2 and ITS/tef1/tub2, in conjunction with morphological characteristics. Five strains isolated from Lithocarpus fohaiensis and Quercus palustris in China represented a new genus of Tubakiaceae, Obovoideisporodochium and three species, viz. Obovoideisporodochium lithocarpi sp. nov., Tubakia lushanensis sp. nov. and T. dryinoides.

HTML

XML

PDF

]]>
Research Article Mon, 22 Nov 2021 10:01:20 +0200
Novel Mucor species (Mucoromycetes, Mucoraceae) from northern Thailand https://mycokeys.pensoft.net/article/71530/ MycoKeys 84: 57-78

DOI: 10.3897/mycokeys.84.71530

Authors: Vedprakash G. Hurdeal, Eleni Gentekaki, Kevin D. Hyde, Thuong T. T. Nguyen, Hyang Burm Lee

Abstract: Mucor species are common fast-growing fungi found in soil. Two new species of Mucor and one new geographical record of M. nederlandicus were collected from northern Thailand and are described in this study. Evidence from morphophysiological data and phylogenetic analysis supports the introduction of the new taxa. Phylogenetic analysis based on the internal transcribed spacer (ITS) and large subunit of the nuclear ribosomal RNA (LSU) data showed that the new isolates cluster distinctly from other Mucor species with high or maximum bootstrap support. Mucor aseptatophorus is characterized by aseptate sporangiophores, globose columella, resistant and deliquescent sporangia, has sympodial, and monopodial branches and shows growth at 37 °C. It also differs from M. irregularis in having smaller sporangiospores, and larger sporangia. Mucor chiangraiensis has subglobose or slightly elongated globose columella, produces hyaline sporangiospores, and resistant and deliquescent sporangia. Furthermore, this species has wider sporangiophore, smaller sporangia and lower growth than M. nederlandicus. A detailed description of the species and illustrations are provided for the novel species.

HTML

XML

PDF

]]>
Research Article Mon, 1 Nov 2021 09:31:53 +0200
Characterization of Diaporthe species on Camellia oleifera in Hunan Province, with descriptions of two new species https://mycokeys.pensoft.net/article/71701/ MycoKeys 84: 15-33

DOI: 10.3897/mycokeys.84.71701

Authors: Qin Yang, Jie Tang, Guo Y. Zhou

Abstract: Tea-oil tree (Camellia oleifera Abel.) is an important edible oil woody plant with a planting area over 3,800,000 hectares in southern China. Species of Diaporthe inhabit a wide range of plant hosts as plant pathogens, endophytes and saprobes. At present, relatively little is known about the taxonomy and genetic diversity of Diaporthe on C. oleifera. Here, we conducted an extensive field survey in Hunan Province in China to identify and characterise Diaporthe species associated with tea-oil leaf spots. As a result, eleven isolates of Diaporthe were obtained from symptomatic C. oleifera leaves. These isolates were studied by applying a polyphasic approach including morphological and phylogenetic analyses of partial ITS, cal, his3, tef1 and tub2 gene regions. Two new Diaporthe species (D. camelliae-oleiferae and D. hunanensis) were proposed and described herein, and C. oleifera was revealed to be new host records of D. hubeiensis and D. sojae. This study indicated there is a potential of more undiscovered Diaporthe species from C. oleifera in China.

HTML

XML

PDF

]]>
Research Article Mon, 18 Oct 2021 11:45:37 +0300
Three new species of Rhytidhysteron (Dothideomycetes, Ascomycota) from Mexico https://mycokeys.pensoft.net/article/68582/ MycoKeys 83: 123-144

DOI: 10.3897/mycokeys.83.68582

Authors: Aurora Cobos-Villagrán, Ricardo Valenzuela, César Hernández-Rodríguez, Rosa Paulina Calvillo-Medina, Lourdes Villa-Tanaca, Luz Elena Mateo-Cid, Abigail Pérez-Valdespino, César Ramiro Martínez-González, Tania Raymundo

Abstract: The genus Rhytidhysteron is characterised by forming navicular to apothecial hysterothecia, exposing the green, yellow, orange, red, vinaceous or black colours of the hymenium which generally releases pigments in the presence of KOH. The exciple is smooth or striated, the asci bitunicate and ascospores have 1–5 transverse septa. To date, twenty-six Rhytidhysteron species have been described from the Tropics. The present study aims to describe three new species in the Neotropics of Mexico based on molecular methods and morphological features. Illustrations and a taxonomic key are provided for all known species of this genus. Rhytidhysteron cozumelense from the Isla Cozumel Biosphere Reserve, R. esperanzae from the Sierra Juárez, Oaxaca and R. mesophilum from the Sierra Madre Oriental, Hidalgo are described as new species. With the present study, the number of species of Rhytidhysteron known from Mexico is now increased to eight.

HTML

XML

PDF

]]>
Review Article Tue, 14 Sep 2021 10:56:44 +0300
Phylogenetic and morphological analyses of Coniochaeta isolates recovered from Inner Mongolia and Yunnan revealed three new endolichenic fungal species https://mycokeys.pensoft.net/article/71140/ MycoKeys 83: 105-121

DOI: 10.3897/mycokeys.83.71140

Authors: Hong-Li Si, Yue-Min Su, Xiao-Xiao Zheng, Meng-Yao Ding, Tanay Bose, Run-Lei Chang

Abstract: Lichens are the result of a symbiotic interaction between fungi (mycobionts) and algae (phycobionts). Aside from mycobionts, lichen thalli can also contain non-lichenised fungal species, such as lichenicolous and endolichenic fungi. For this study, three surveys were conducted in China’s Yunnan Province and Inner Mongolia Autonomous Region between 2017 and 2020. Several samples of four lichen species were collected during these surveys: Candelaria fibrosa, Flavoparmelia caperata, Flavopunctelia flaventior and Ramalina sinensis. Six isolates of Coniochaeta were recovered from these four lichen species. The phylogenetic and morphological analyses revealed that two of these isolates were previously identified species, Coniochaeta velutinosa and C. acaciae. Those remaining were from potentially unknown species. We used molecular and morphological data to describe these previously-unknown species as Coniochaeta fibrosae sp. nov., C. mongoliae sp. nov. and C. sinensis sp. nov. The findings of this study significantly improve our understanding of the variety and habitat preferences of Coniochaeta in China and globally.

HTML

XML

PDF

]]>
Research Article Thu, 9 Sep 2021 16:09:43 +0300
Greetings from belowground: two new species of truffles in the genus Pachyphlodes (Pezizaceae, Pezizales) from México https://mycokeys.pensoft.net/article/67685/ MycoKeys 82: 159-171

DOI: 10.3897/mycokeys.82.67685

Authors: Carolina Piña Páez, Rosanne A. Healy, Gonzalo Guevara, Roberto Garibay Orijel, Michael A. Castellano, Efrén Cázares, James M. Trappe

Abstract: Pachyphlodes is a lineage of ectomycorrhizal, hypogeous, sequestrate ascomycete fungi native to temperate and subtropical forests in the Northern Hemisphere. Pachyphlodes species form ectomycorrhizae mainly with Fagales hosts. Here we describe two new species of Pachyphlodes, P. brunnea, and P. coalescens, based on morphological and phylogenetic analysis. Pachyphlodes brunnea is distributed in the states of Tamaulipas and Nuevo León in northern México, occurring with Quercus and Juglans species. It is characterized by its dark brown peridium, white gleba, and spores with capitate columns. Pachyphlodes coalescens is distributed in the states of Michoacán and Tlaxcala in central and southwestern México co-occurring with Quercus and is distinguished by its reddish-brown peridium, light yellow gleba, and spore ornamentation. Both species, along with P. marronina, constitute the Marronina clade. This clade contains North American species characterized by a brown peridium and spores ornamented with capitate spines to coalesced spine tips that form a partial perispore.

HTML

XML

PDF

]]>
Research Article Tue, 17 Aug 2021 12:51:57 +0300
Novel saprobic Hermatomyces species (Hermatomycetaceae, Pleosporales) from China (Yunnan Province) and Thailand https://mycokeys.pensoft.net/article/67973/ MycoKeys 82: 57-79

DOI: 10.3897/mycokeys.82.67973

Authors: Guang-Cong Ren, Dhanushka N. Wanasinghe, Jutamart Monkai, Peter E. Mortimer, Kevin D. Hyde, Jian-Chu Xu, Aimin Pang, Heng Gui

Abstract: During our survey of the diversity of woody litter fungi in China and Thailand, three Hermatomyces species were collected from dead woody twigs of Dipterocarpus sp. (Dipterocarpaceae) and Ehretia acuminata (Boraginaceae). Both morphology and multigene analyses revealed two taxa as new species (Hermatomyces turbinatus and H. jinghaensis) and the remaining collections as new records of H. sphaericus. Hermatomyces turbinatus is characterized by 1) dimorphic conidia, having circular to oval lenticular conidia and 2) turbinate conidia consisting of two columns with two septa composed of 2–3 cells in each column. Hermatomyces jinghaensis is characterized by dimorphic conidia, having circular to oval lenticular conidia and clavate or subcylindrical to cylindrical conidia and consisting of one or two columns with 6–8 cells in each column. Phylogenetic analyses of combined LSU, ITS, tub2, tef1-α and rpb2 sequence data supports the placement of these new taxa within Hermatomycetaceae with high statistical support.

HTML

XML

PDF

]]>
Research Article Mon, 9 Aug 2021 15:36:43 +0300
Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota) https://mycokeys.pensoft.net/article/63241/ MycoKeys 82: 33-56

DOI: 10.3897/mycokeys.82.63241

Authors: Long-Fei Fan, Renato Lúcio Mendes Alvarenga, Tatiana Baptista Gibertoni, Fang Wu, Yu-Cheng Dai

Abstract: Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species (T. cheejenii, T. fibulifera s.s., T. “neofibulifera”, T. lloydiae-candidae and T. olens) and four new species (T. australe, T. guangxiensis, T. latispora and T. subfibulifera) in the complex are recognized based on morphological characteristics, molecular evidence, and geographic distribution. Sequences of eight species of the complex were included in the phylogenetic analyses because T. olens lacks molecular data. The phylogenetic analyses were performed by a combined sequence dataset of the internal transcribed spacer (ITS) and the partial nuclear large subunit rDNA (nLSU), and a combined sequence dataset of the ITS, partial nLSU, the small subunit mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α (TEF1), the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2). The eight species formed eight independent lineages with robust support in phylogenies based on both datasets. Illustrated description of the six species including Tremella fibulifera s.s., T. “neofibulifera” and four new species, and discussions with their related species, are provided. A table of the comparison of the important characteristics of nine species in the T. fibulifera complex and a key to the whitish species in Tremella s.s. are provided.

HTML

XML

PDF

]]>
Research Article Wed, 4 Aug 2021 15:09:13 +0300
Unravelling unexplored diversity of cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) in tropical Africa https://mycokeys.pensoft.net/article/67850/ MycoKeys 81: 69-138

DOI: 10.3897/mycokeys.81.67850

Authors: Yalemwork Meswaet, Ralph Mangelsdorff, Nourou S. Yorou, Meike Piepenbring

Abstract: Cercosporoid fungi (Mycosphaerellaceae, Mycosphaerellales, Ascomycota) are one of the largest and most diverse groups of hyphomycetes causing a wide range of diseases of economically important plants as well as of plants in the wild. Although more than 6000 species are known for this group, the documentation of this fungal group is far from complete. Especially in the tropics, the diversity of cercosporoid fungi is poorly known. The present study aims to identify and characterise cercosporoid fungi collected on host plants belonging to Fabaceae in Benin, West Africa. Information on their morphology, host species and DNA sequence data (18S rDNA, 28S rDNA, ITS and tef1) is provided. DNA sequence data were obtained by a simple and non-culture-based method for DNA isolation which has been applied for cercosporoid fungi for the first time in the context of the present study. Among the loci used for the phylogenetic analysis, tef1 provided the best resolution together with the multigene dataset. Species delimitation in many cases, however, was only possible by combining molecular sequence data with morphological characteristics. Based on forty specimens recently collected in Benin, 18 species are presented with morphological descriptions, illustrations and sequence data. Among these, six species in the genus Cercospora and two species in Pseudocercospora are proposed as species new to science. The newly described species are Cercospora (C.) beninensis on Crotalaria macrocalyx, C. parakouensis on Desmodium tortuosum, C. rhynchophora on Vigna unguiculata, C. vignae-subterraneae on Vigna subterranea, C. tentaculifera on Vigna unguiculata, C. zorniicola on Zornia glochidiata, Pseudocercospora sennicola on Senna occidentalis and Pseudocercospora tabei on Vigna unguiculata. Eight species of cercosporoid fungi are reported for Benin for the first time, three of them, namely C. cf. canscorina, C. cf. fagopyri and C. phaseoli-lunati are new for West Africa. The presence of two species of cercosporoid fungi on Fabaceae previously reported from Benin, namely Nothopassalora personata and Passalora arachidicola, is confirmed.

HTML

XML

PDF

]]>
Research Article Thu, 17 Jun 2021 08:13:43 +0300
Phylogeny and taxonomy of Catenularia and similar fungi with catenate conidia https://mycokeys.pensoft.net/article/67785/ MycoKeys 81: 1-44

DOI: 10.3897/mycokeys.81.67785

Authors: Martina Réblová, Jana Nekvindová, Andrew N. Miller

Abstract: The genus Catenularia (Chaetosphaeriaceae) was reviewed, and its relationships with morphologically similar fungi were evaluated using molecular and morphological data. Eleven species are accepted, four of which have been verified with molecular DNA data. The correct epithet ‘cupulifera’ is proposed for the type species C. cupulifera comb. nov. Four other combinations are proposed, namely C. catenulata comb. nov., C. elsikii comb. nov., C. minor comb. nov. and C. novae-zelandiae comb. nov. Catenularia is an uncommon fungus inhabiting mainly decaying bark, wood and bamboo culms of various hosts and shows a widespread geographical distribution. It is circumscribed for fungi with mononematous, macronematous, simple conidiophores with terminal monophialides, usually accompanied with capitate hyphae. The conidia are aseptate, brown, cuneiform to rounded-obconic with an angular outline, adhering in chains. The diagnostic values of taxonomic characteristics of capitate hyphae and conidia (i.e. colour, shape in transverse section, setulae and formation) at the generic level were evaluated. An account of morphology, taxonomy and phylogeny of species accepted in Catenularia is provided. Based on ribosomal DNA sequences, Chalarodes obpyramidata sp. nov., characterised by catenate, angular, hyaline conidia with apical setulae, is revealed as closely related to Catenularia. The new genus Fuscocatenula gen. nov. is proposed for catenularia-like fungi having pigmented conidia with protracted maturation and round outline, with two species accepted, F. submersa comb. nov. and F. variegata comb. nov. A new species Nawawia antennata sp. nov. is introduced and Nawawia is compared with morphologically similar taxa.

HTML

XML

PDF

]]>
Research Article Fri, 11 Jun 2021 08:43:53 +0300
Three new Melanoleuca species (Agaricales, Basidiomycota) from north-eastern China, supported by morphological and molecular data https://mycokeys.pensoft.net/article/64369/ MycoKeys 80: 133-148

DOI: 10.3897/mycokeys.80.64369

Authors: Ying Pei, Hong-Bo Guo, Tie-Zhi Liu, Wei-Qiang Qin, Di Zhao, Xiao-Jian Qi, Xiao-Dan Yu

Abstract: Three new Melanoleuca species, M. chifengense, M. griseoflava and M. pallidorosea, were discovered in the northeast of China. Melanoleuca chifengense is morphologically characterised by its grey to yellowish-grey pileus, decurrent lamellae, grey to yellowish-brown stipe, yellowish-grey context, ellipsoid basidiospores with irregular warts and lack of hymenial cystidia. Melanoleuca griseoflava is mainly characterised by its greyish-brown pileus, adnexed to adnate lamellae, greyish-yellow context, fusiform cystidia and almost reticulate basidiospores. Melanoleuca pallidorosea is characterised by its pinkish-white pileus, white and decurrent lamellae, ellipsoid basidiospores with round and scattered warts and lack of hymenial cystidia. The phylogenetic relationship of the three species was determined by the analyses of the ITS region and the combined data matrix (ITS-nrLSU-RPB2), respectively. The results showed that the three species formed three independent lineages. Based on the combination of both morphological and molecular data, M. chifengense, M. griseoflava and M. pallidorosea were confirmed to be new species. The morphological similarities of the three new species is also discussed.

HTML

XML

PDF

]]>
Research Article Thu, 3 Jun 2021 13:10:56 +0300
Two new species and a new record of yellow Cantharellus from tropical Quercus forests in eastern Mexico with the proposal of a new name for the replacement of Craterellus confluens https://mycokeys.pensoft.net/article/61443/ MycoKeys 80: 91-114

DOI: 10.3897/mycokeys.80.61443

Authors: Leticia Montoya, Mariana Herrera, Victor M. Bandala, Antero Ramos

Abstract: Two new species of yellow Cantharellus and a new record of Cantharellus tabernensis associated with tropical species of Quercus are presented, based on the taxonomic study of fresh specimens and in a phylogenetic analysis of transcription elongation factor 1-alpha (tef-1α) and the large subunit of the ribosome (nLSU) sequences. One of the new species proposed here, corresponds to a choice edible mushroom, which, in our molecular phylogeny, resulted in it being related to the group of species around C. lateritius and sister with Craterellus confluens type specimen. This latter is here formally transferred to Cantharellus and consequently a new name, Cantharellus furcatus, is proposed to replace the homonym Cantharellus confluens (Schwein.) Schwein. 1834 a later synonym of Byssomerulius corium. Detailed macroscopic and microscopic descriptions accompanied with illustrations and a taxonomic discussion are presented for each species.

HTML

XML

PDF

]]>
Research Article Thu, 20 May 2021 15:33:00 +0300
Multi-gene phylogenetic evidence indicates that Pleurodesmospora belongs in Cordycipitaceae (Hypocreales, Hypocreomycetidae) and Pleurodesmospora lepidopterorum sp. nov. on pupa from China https://mycokeys.pensoft.net/article/66794/ MycoKeys 80: 45-55

DOI: 10.3897/mycokeys.80.66794

Authors: Wan-Hao Chen, Yan-Feng Han, Jian-Dong Liang, Wei-Yi Tian, Zong-Qi Liang

Abstract: A new species, Pleurodesmospora lepidopterorum, isolated from a pupa, is introduced. Morphological comparisons and phylogenetic analyses based on multigene datasets (ITS+RPB1+RPB2+TEF) support the establishment of the new species. Pleurodesmospora lepidopterorum is distinguished from P. coccorum by its longer conidiogenous pegs located in the terminal or lateral conidiophores, and smaller subglobose or ellipsoidal conidia. A combined dataset of RPB1, RPB2, and TEF confirmed the taxonomic placement of Pleurodesmospora in Cordycipitaceae for the first time.

HTML

XML

PDF

]]>
Research Article Fri, 7 May 2021 12:52:08 +0300
Morpho-molecular characterization of Discosia ravennica sp. nov. and a new host record for Sporocadus rosigena https://mycokeys.pensoft.net/article/60662/ MycoKeys 79: 173-192

DOI: 10.3897/mycokeys.79.60662

Authors: Digvijayini Bundhun, Rajesh Jeewon, Indunil C. Senanayake, Erio Camporesi, Janith V. S. Aluthmuhandiram, Alvin M. C. Tang, Ji-Chuan Kang, Vishwakalyan Bhoyroo, Kevin D. Hyde

Abstract: Collections of fungal samples from two dead leaf specimens from Italy were subjected to morphological examination and phylogenetic analyses. Two coelomycetous taxa belonging to two different genera in Xylariomycetidae, Sordariomycetes, namely Discosia and Sporocadus, were identified. The Discosia taxon is revealed as a new species and is herein introduced as Discosia ravennica sp. nov. while the Sporocadus taxon is identified as Sporocadus rosigena. Multi-locus phylogeny based on DNA sequence data of the large subunit (LSU) and internal transcribed spacer (ITS) of nuclear ribosomal genes, β-tubulin (β-tub) and RNA polymerase II second largest subunit (rpb2) showed that D. ravennica is related to D. neofraxinea but it forms an independent lineage that supports its new species status. The new taxon also differs from other Discosia species by its unilocular to bilocular, superficial and applanate conidiomata with basal stroma composed of cells of textura angularis, elongate-ampulliform conidiogenous cells and conidia smaller in size. Sporocadus rosigena is here reported as a new host record from Quercus ilex from Italy. Descriptions, illustrations and molecular data for both species are provided in this paper.

HTML

XML

PDF

]]>
Research Article Tue, 27 Apr 2021 07:54:51 +0300
Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China https://mycokeys.pensoft.net/article/60878/ MycoKeys 78: 49-77

DOI: 10.3897/mycokeys.78.60878

Authors: Shengting Huang, Jiwen Xia, Xiuguo Zhang, WenXiu Sun

Abstract: Species of Diaporthe have often been reported as plant pathogens, endophytes or saprobes, commonly isolated from a wide range of plant hosts. Sixteen strains isolated from species of ten host genera in Yunnan Province, China, represented three new species of Diaporthe, D. chrysalidocarpi, D. machili and D. pometiae as well as five known species D. arecae, D. hongkongensis, D. middletonii, D. osmanthi and D. pandanicola. Morphological comparisons with known species and DNA-based phylogenies based on the analysis of a multigene (ITS, TUB, TEF, CAL and HIS) dataset support the establishment of the new species. This study reveals that a high species diversity of Diaporthe with wide host ranges occur in tropical rainforest in Yunnan Province, China.

HTML

XML

PDF

]]>
Research Article Fri, 19 Feb 2021 15:35:25 +0200
Notes on Trochila (Ascomycota, Leotiomycetes), with new species and combinations https://mycokeys.pensoft.net/article/62046/ MycoKeys 78: 21-47

DOI: 10.3897/mycokeys.78.62046

Authors: Paula Andrea Gómez-Zapata, Danny Haelewaters, Luis Quijada, Donald H. Pfister, M. Catherine Aime

Abstract: Studies of Trochila (Leotiomycetes, Helotiales, Cenangiaceae) are scarce. Here, we describe two new species based on molecular phylogenetic data and morphology. Trochila bostonensis was collected at the Boston Harbor Islands National Recreation Area, Massachusetts. It was found on the stem of Asclepias syriaca, representing the first report of any Trochila species from a plant host in the family Apocynaceae. Trochila urediniophila is associated with the uredinia of the rust fungus Cerotelium fici. It was discovered during a survey for rust hyperparasites conducted at the Arthur Fungarium, in a single sample from 1912 collected in Trinidad. Macro- and micromorphological descriptions, illustrations, and molecular phylogenetic analyses are presented. The two new species are placed in Trochila with high support in both our six-locus (SSU, ITS, LSU, rpb1, rpb2, tef1) and two-locus (ITS, LSU) phylogenetic reconstructions. In addition, two species are combined in Trochila: Trochila colensoi (formerly placed in Pseudopeziza) and T. xishuangbanna (originally described as the only species in Calycellinopsis). This study reveals new host plant families, a new ecological strategy, and a new country record for the genus Trochila. Finally, our work emphasizes the importance of specimens deposited in biological collections such as fungaria.

HTML

XML

PDF

]]>
Research Article Thu, 11 Feb 2021 07:53:03 +0200
Hebeloma in the Malay Peninsula: Masquerading within Psathyrella https://mycokeys.pensoft.net/article/57394/ MycoKeys 77: 117-141

DOI: 10.3897/mycokeys.77.57394

Authors: Ursula Eberhardt, Nicole Schütz, Henry J. Beker, Su See Lee, Egon Horak

Abstract: In 1994 Corner published five new species within the genus Psathyrella, all having been collected on the Malay Peninsula between 1929 and 1930. Three of these species belong to the genus Hebeloma and with their vinaceous colored lamellae and spore print, when fresh, they belong to H. sect. Porphyrospora. Of these three species, only one, P. flavidifolia, was validly published and thus we herewith recombine it as H. flavidifolium. The other two species, P. splendens and P. verrucispora, are synonyms of H. parvisporum and H. lactariolens, respectively. We also describe a new Malayan species, H. radicans, which also belongs to H. sect. Porphyrospora. These findings confirm the western Pacific Rim as a diversity hotspot for H. sect. Porphyrospora. The records described within this paper, represent the first recognition that the genus Hebeloma, and indeed that members of the ectomycorrhizal Hymenogastraceae, are present on the Malay Peninsula.

HTML

XML

PDF

]]>
Research Article Thu, 28 Jan 2021 13:36:21 +0200
Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species https://mycokeys.pensoft.net/article/59852/ MycoKeys 77: 65-95

DOI: 10.3897/mycokeys.77.59852

Authors: Wenxiu Sun, Shengting Huang, Jiwen Xia, Xiuguo Zhang, Zhuang Li

Abstract: Diaporthe species have often been reported as plant pathogens, endophytes and saprophytes, commonly isolated from a wide range of infected plant hosts. In the present study, twenty strains obtained from leaf spots of twelve host plants in Yunnan Province of China were isolated. Based on a combination of morphology, culture characteristics and multilocus sequence analysis of the rDNA internal transcribed spacer region (ITS), translation elongation factor 1-α (TEF), β-tubulin (TUB), calmodulin (CAL), and histone (HIS) genes, these strains were identified as eight new species: Diaporthe camelliae-sinensis, D. grandiflori, D. heliconiae, D. heterostemmatis, D. litchii, D. lutescens, D. melastomatis, D. pungensis and two previously described species, D. subclavata and D. tectonendophytica. This study showed high species diversity of Diaporthe in tropical rain forests and its hosts in south-western China.

HTML

XML

PDF

]]>
Review Article Thu, 14 Jan 2021 16:58:23 +0200
New species and records of Diaporthe from Jiangxi Province, China https://mycokeys.pensoft.net/article/59999/ MycoKeys 77: 41-64

DOI: 10.3897/mycokeys.77.59999

Authors: Qin Yang, Ning Jiang, Cheng-Ming Tian

Abstract: Diaporthe species have often been reported as important plant pathogens, saprobes and endophytes on a wide range of plant hosts. Although several Diaporthe species have been recorded, little is known about species able to infect forest trees in Jiangxi Province. Hence, extensive surveys were recently conducted in Jiangxi Province, China. A total of 24 isolates were identified and analysed using comparisons of DNA sequence data for the nuclear ribosomal internal transcribed spacer (ITS), calmodulin (cal), histone H3 (his3), partial translation elongation factor-1α (tef1) and β-tubulin (tub2) gene regions, as well as their morphological features. Results revealed five novel taxa, D. bauhiniae, D. ganzhouensis, D. schimae, D. verniciicola, D. xunwuensis spp. nov. and three known species, D. apiculatum, D. citri and D. multigutullata.

HTML

XML

PDF

]]>
Research Article Thu, 14 Jan 2021 15:07:17 +0200
Phialolunulospora vermispora (Chaetosphaeriaceae, Sordariomycetes), a novel asexual genus and species from freshwater in southern China https://mycokeys.pensoft.net/article/57410/ MycoKeys 76: 17-30

DOI: 10.3897/mycokeys.76.57410

Authors: Hua Zheng, Yake Wan, Jie Li, Rafael F. Castañeda-Ruiz, Zefen Yu

Abstract: The asexual taxon Phialolunulospora vermispora gen. et sp. nov., collected from submerged dicotyledonous leaves in Hainan, China, is described and illustrated herein. Phialolunulospora gen. nov. is characterized by macronematous, semimacronematous, septate and pigmented conidiophores and acrogenous, long lunate, vermiform to sigmoid, hyaline conidia with an eccentric basal appendage. Complete sequences of internal transcribed spacer (ITS) and partial sequences of nuclear large subunits ribosomal DNA (LSU) genes are provided. Phylogenetic analyses of combined ITS and LSU sequences revealed its placement in the Chaetosphaeriaceae. The new fungus is compared with morphologically similar genera.

HTML

XML

PDF

]]>
Research Article Tue, 22 Dec 2020 13:56:18 +0200
The taxonomy of the model filamentous fungus Podospora anserina https://mycokeys.pensoft.net/article/55968/ MycoKeys 75: 51-69

DOI: 10.3897/mycokeys.75.55968

Authors: S. Lorena Ament-Velásquez, Hanna Johannesson, Tatiana Giraud, Robert Debuchy, Sven J. Saupe, Alfons J. M. Debets, Eric Bastiaans, Fabienne Malagnac, Pierre Grognet, Leonardo Peraza-Reyes, Pierre Gladieux, Åsa Kruys, Philippe Silar, Sabine M. Huhndorf, Andrew N. Miller, Aaron A. Vogan

Abstract: The filamentous fungus Podospora anserina has been used as a model organism for more than 100 years and has proved to be an invaluable resource in numerous areas of research. Throughout this period, P. anserina has been embroiled in a number of taxonomic controversies regarding the proper name under which it should be called. The most recent taxonomic treatment proposed to change the name of this important species to Triangularia anserina. The results of past name changes of this species indicate that the broader research community is unlikely to accept this change, which will lead to nomenclatural instability and confusion in literature. Here, we review the phylogeny of the species closely related to P. anserina and provide evidence that currently available marker information is insufficient to resolve the relationships amongst many of the lineages. We argue that it is not only premature to propose a new name for P. anserina based on current data, but also that every effort should be made to retain P. anserina as the current name to ensure stability and to minimise confusion in scientific literature. Therefore, we synonymise Triangularia with Podospora and suggest that either the type species of Podospora be moved to P. anserina from P. fimiseda or that all species within the Podosporaceae be placed in the genus Podospora.

HTML

XML

PDF

]]>
Research Article Wed, 25 Nov 2020 10:16:39 +0200
Cortinarius pakistanicus and C. pseudotorvus: two new species in oak forests in the Pakistan Himalayas https://mycokeys.pensoft.net/article/49734/ MycoKeys 74: 91-108

DOI: 10.3897/mycokeys.74.49734

Authors: Arooj Naseer, Isaac Garrido-Benavent, Junaid Khan, Josep Ballarà, Rafael Mahiques, Abdul Nasir Khalid, Hassan Sher

Abstract: The genus of basidiomycetous fungi Cortinarius occurs worldwide, from subtropical to boreal latitudes. Although molecular systematics has triggered the study of these fungi in the Americas and Europe in the last two decades, there is still limited research on its diversity in large portions of the planet, such as the high mountain ranges of Asia. Several collections of Cortinarius were made during mycological field trips conducted between 2014 and 2018 in pure oak forests in the Pakistan Himalayas. An integrative framework combining morphological and phylogenetic data was employed for their study. As a result, the two species C. pakistanicus and C. pseudotorvus are here described as new to science. Detailed macro- and micro-morphological descriptions, including SEM images of spores, and a molecular phylogenetic reconstruction based on nrITS sequence data are provided and used to discriminate the new species from morphologically and phylogenetically close taxa. Whereas our phylogenetic tree inference gave unequivocal support for the inclusion of C. pseudotorvus within C. sect. Telamonia, the assignment of C. pakistanicus to any known sections remained elusive. These species likely establish ectomycorrhizal associations with trees in the genus Quercus, making this type of forest in the Pakistan Himalayas a promising focus for future research on the diversity of Cortinarius.

HTML

XML

PDF

]]>
Research Article Fri, 30 Oct 2020 16:34:43 +0200
Corrigendum: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1–68. https://doi.org/10.3897/mycokeys.73.47287 https://mycokeys.pensoft.net/article/59175/ MycoKeys 74: 109-110

DOI: 10.3897/mycokeys.74.59175

Authors: Richard Spjut, Antoine Simon, Martin Guissard, Nicolas Magain, Emmanuël Sérusiaux

Abstract: None

HTML

XML

PDF

]]>
Corrigenda Fri, 30 Oct 2020 08:55:29 +0200
Delimitation, new species and teleomorph-anamorph relationships in Codinaea, Dendrophoma, Paragaeumannomyces and Striatosphaeria (Chaetosphaeriaceae) https://mycokeys.pensoft.net/article/57824/ MycoKeys 74: 17-74

DOI: 10.3897/mycokeys.74.57824

Authors: Martina Réblová, Jana Nekvindová, Jacques Fournier, Andrew N. Miller

Abstract: The Chaetosphaeriaceae are a diverse group of pigmented, predominantly phialidic hyphomycetes comprised of several holomorphic genera including Chaetosphaeria, the most prominent genus of the family. Although the morphology of the teleomorphs of the majority of Chaetosphaeria is rather uniform, their associated anamorphs primarily exhibit the variability and evolutionary change observed in the genus. An exception from the morphological monotony among Chaetosphaeria species is a group characterised by scolecosporous, hyaline to light pink, multiseptate, asymmetrical ascospores and a unique three-layered ascomatal wall. Paragaeumannomyces sphaerocellularis, the type species of the genus, exhibits these morphological traits and is compared with similar Chaetosphaeria with craspedodidymum- and chloridium-like synanamorphs. Morphological comparison and phylogenetic analyses of the combined ITS-28S sequences of 35 isolates and vouchers with these characteristics revealed a strongly-supported, morphologically well-delimited clade in the Chaetosphaeriaceae containing 16 species. The generic name Paragaeumannomyces is applied to this monophyletic clade; eight new combinations and five new species, i.e. P. abietinus sp. nov., P. elegans sp. nov., P. granulatus sp. nov., P. sabinianus sp. nov. and P. smokiensis sp. nov., are proposed. A key to Paragaeumannomyces is provided. Using morphology, cultivation studies and phylogenetic analyses of ITS and 28S rDNA, two additional new species from freshwater and terrestrial habitats, Codinaea paniculata sp. nov. and Striatosphaeria castanea sp. nov., are described in the family. A codinaea-like anamorph of S. castanea forms conidia with setulae at each end in axenic culture; this feature expands the known morphology of Striatosphaeria. A chaetosphaeria-like teleomorph is experimentally linked to Dendrophoma cytisporoides, a sporodochial hyphomycete and type species of Dendrophoma, for the first time.

HTML

XML

PDF

]]>
Research Article Mon, 19 Oct 2020 16:50:49 +0300
Morphological and phylogenetic characterisations reveal three new species of Samsoniella (Cordycipitaceae, Hypocreales) from Guizhou, China https://mycokeys.pensoft.net/article/56655/ MycoKeys 74: 1-15

DOI: 10.3897/mycokeys.74.56655

Authors: Wan-Hao Chen, Yan-Feng Han, Jian-Dong Liang, Wei-Yi Tian, Zong-Qi Liang

Abstract: Samsoniella species have been found on lepidopteran larvae or pupae buried in soil or leaf litter. Three new species, Samsoniella hymenopterorum, S. coleopterorum and S. lepidopterorum, parasitic on hymenopteran larvae, coleopteran larvae and lepidopteran pupae, respectively, are reported. Morphological comparisons with extant species and DNA-based phylogenies from analysis of a multigene (ITS, RPB1, RPB2 and TEF) dataset supported the establishment of the new species. Unusually, all three new species have mononematous conidiophores. The new species are clearly distinct from other species in Samsoniella occurring in separate subclades.

HTML

XML

PDF

]]>
Research Article Mon, 19 Oct 2020 16:42:34 +0300
Three new species of Conidiobolus sensu stricto from plant debris in eastern China https://mycokeys.pensoft.net/article/56905/ MycoKeys 73: 133-149

DOI: 10.3897/mycokeys.73.56905

Authors: Yong Nie, Yue Cai, Yang Gao, De-Shui Yu, Zi-Min Wang, Xiao-Yong Liu, Bo Huang

Abstract: The genus Conidiobolus Bref. is widely distributed and the Conidiobolus sensu lato contained three other genera, Capillidium, Microconidiobolus and Neoconidiobolus. A molecular phylogeny based on the nuclear large subunit of rDNA (nucLSU), the mitochondrial small subunit of rDNA (mtSSU) and the translation elongation factor 1-alpha gene (TEF1) revealed three novel species within the clade of Conidiobolus s.s., i.e. C. bifurcatus sp. nov., C. taihushanensis sp. nov. and C. variabilis sp. nov. These three species were isolated from plant debris in eastern China. Morphologically, C. bifurcatus sp. nov. is characterised by its secondary conidiophores often branched at the tip to form two short stipes each bearing a secondary conidium. C. taihushanensis sp. nov. is different from the others in its straight apical mycelia and the production of 2–5 conidia. C. variabilis sp. nov. is distinctive because of its various shapes of primary conidia. All these three new taxa are illustrated herein with an update key to the species of the genus Conidiobolus s.s.

HTML

XML

PDF

]]>
Research Article Thu, 8 Oct 2020 11:11:34 +0300
The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history https://mycokeys.pensoft.net/article/47287/ MycoKeys 73: 1-68

DOI: 10.3897/mycokeys.73.47287

Authors: Richard Spjut, Antoine Simon, Martin Guissard, Nicolas Magain, Emmanuël Sérusiaux

Abstract: We present phylogenetic analyses of the fruticose Ramalinaceae based on extensive collections from many parts of the world, with a special focus on the Vizcaíno deserts in north-western Mexico and the coastal desert in Namibia. We generate a four-locus DNA sequence dataset for accessions of Ramalina and two additional loci for Niebla and Vermilacinia. Four genera are strongly supported: the subcosmopolitan Ramalina, the new genus Namibialina endemic to SW Africa, and a duo formed by Niebla and Vermilacinia, endemic to the New World except the sorediate V. zebrina that disjunctly occurs in Namibia. The latter three genera are restricted to coastal desert and chaparral where vegetation depends on moisture from ocean fog. Ramalina is subcosmopolitan and much more diverse in its ecology. We show that Ramalina and its sister genus Namibialina diverged from each other at c. 48 Myrs, whereas Vermilacinia and Niebla split at c. 30 Myrs. The phylogeny of the fruticose genera remains unresolved to their ancestral crustose genera. Species delimitation within Namibialina and Ramalina is rather straightforward. The phylogeny and taxonomy of Vermilacinia are fully resolved, except for the two youngest clades of corticolous taxa, and support current taxonomy, including four new taxa described here. Secondary metabolite variation in Niebla generally coincides with major clades which are comprised of species complexes with still unresolved phylogenetic relationships. A micro-endemism pattern of allopatric species is strongly suspected for both genera, except for the corticolous taxa within Vermilacinia. Both Niebla and saxicolous Vermilacinia have chemotypes unique to species clades that are largely endemic to the Vizcaíno deserts. The following new taxa are described: Namibialina gen. nov. with N. melanothrix (comb. nov.) as type species, a single new species of Ramalina (R. krogiae) and four new species of Vermilacinia (V. breviloba, V. lacunosa, V. pustulata and V. reticulata). The new combination V. granulans is introduced. Two epithets are re-introduced for European Ramalina species: R. crispans (= R. peruviana auct. eur.) and R. rosacea (= R. bourgeana auct. p.p). A lectotype is designated for Vermilacinia procera. A key to saxicolous species of Vermilacinia is presented.

HTML

XML

PDF

]]>
Research Article Fri, 11 Sep 2020 14:49:22 +0300
Neodactylariales, Neodactylariaceae (Dothideomycetes, Ascomycota): new order and family, with a new species from China https://mycokeys.pensoft.net/article/54054/ MycoKeys 73: 69-85

DOI: 10.3897/mycokeys.73.54054

Authors: Min Qiao, Hua Zheng, Ruili Lv, Zefen Yu

Abstract: During a mycological survey of aquatic hyphomycetes on submerged decaying leaves in southwest China, a distinct new fungus was collected. The collection was cultured and sequenced and a BLAST search of its ITS and LSU sequence against data in GenBank revealed a dothideomycetous affiliation, with the closest related taxa in the genus Neodactylaria. Phylogenetic analyses of a multigene matrix containing sequences from four genes (LSU, SSU, rpb2, and tef1), representing broad groups of Dothideomycetes, revealed its placement within Dothideomycetes, but without a supported familial or ordinal affiliation. Based on further phylogenetic analyses and morphological investigations, the new fungus is described here as a new species of Neodactylaria, N. simaoensis sp. nov., and placed in a new family Neodactylariaceae fam. nov. and a new order Neodactylariales ord. nov.

HTML

XML

PDF

]]>
Research Article Fri, 11 Sep 2020 08:57:22 +0300
Phylogeny- and morphology-based recognition of new species in the spider-parasitic genus Gibellula (Hypocreales, Cordycipitaceae) from Thailand https://mycokeys.pensoft.net/article/55088/ MycoKeys 72: 17-42

DOI: 10.3897/mycokeys.72.55088

Authors: Wilawan Kuephadungphan, Kanoksri Tasanathai, Booppa Petcharad, Artit Khonsanit, Marc Stadler, J. Jennifer Luangsa-ard

Abstract: Thailand is known to be a part of what is called the Indo-Burma biodiversity hotspot, hosting a vast array of organisms across its diverse ecosystems. This is reflected by the increasing number of new species described over time, especially fungi. However, a very few fungal species from the specialized spider-parasitic genus Gibellula have ever been reported from this region. A survey of invertebrate-pathogenic fungi in Thailand over several decades has led to the discovery of a number of fungal specimens with affinities to this genus. Integration of morphological traits into multi-locus phylogenetic analysis uncovered four new species: G. cebrennini, G. fusiformispora, G. pigmentosinum, and G. scorpioides. All these appear to be exclusively linked with torrubiella-like sexual morphs with the presence of granulomanus-like asexual morph in G. pigmentosinum and G. cebrennini. A remarkably high host specificity of these new species towards their spider hosts was revealed, and for the first time, evidence is presented for manipulation of host behavior in G. scorpioides.

HTML

XML

PDF

]]>
Research Article Wed, 2 Sep 2020 08:23:13 +0300
Three new species of Junghuhnia (Polyporales, Basidiomycota) from China https://mycokeys.pensoft.net/article/51872/ MycoKeys 72: 1-16

DOI: 10.3897/mycokeys.72.51872

Authors: Ping Du, Wu Fang, Xue-Mei Tian

Abstract: In this study, taxonomic and phylogenetic analyses of Junghuhnia were performed. Three new species were characterised according to morphological characteristics and molecular phylogenetic analysis using ITS and nLSU sequences. They are J. austrosinensis sp. nov., J. nandinae sp. nov. and J. subcollabens sp. nov. Junghuhnia austrosinensis is characterised by resupinate, thin basidiomata with white to buff-yellow hymenophore, small pores (9–11 per mm), clamped generative hyphae possessing hymenial cystidia, ellipsoid basidiospores (2.5–3 × 1.7–2 µm) and growth on fallen bamboo or angiosperm branch. Junghuhnia nandinae is characterised by resupinate basidiomata with pink to salmon pores and a distinct white margin, clamp generative hyphae, interwoven tramal hyphae, ellipsoid basidiospores measuring 2.6–3.2 × 1.8–2 µm and growth on Nandina domestica. Junghuhnia subcollabens is characterised by resupinate basidiomata with pale salmon to brownish vinaceous hymenophore, small pores (10–12 per mm), generative hyphae with simple septa and clamp connections, interwoven tramal hyphae, lunate basidiospores measuring 2.9–3.4 × 1.6–1.8 µm and thriving on rotten wood of angiosperms.

HTML

XML

PDF

]]>
Research Article Fri, 14 Aug 2020 08:46:56 +0300
Multi-gene phylogenetic evidence suggests Dictyoarthrinium belongs in Didymosphaeriaceae (Pleosporales, Dothideomycetes) and Dictyoarthrinium musae sp. nov. on Musa from Thailand https://mycokeys.pensoft.net/article/55493/ MycoKeys 71: 101-118

DOI: 10.3897/mycokeys.71.55493

Authors: Binu C. Samarakoon, Dhanushka N. Wanasinghe, Milan C. Samarakoon, Rungtiwa Phookamsak, Eric H.C. McKenzie, Putarak Chomnunti, Kevin D. Hyde, Saisamorn Lumyong, Samantha C. Karunarathna

Abstract: Dead leaves of Musa sp. (banana) were collected in northern Thailand during an investigation of saprobic fungi. Preliminary morphological observations revealed that three specimens belong to Dictyoarthrinium. Phylogenetic analyses of combined SSU, LSU, ITS and tef1-α sequence data revealed that Dictyoarthrinium forms a clade in Didymosphaeriaceae (Massarineae, Pleosporales, Dothideomycetes) sister to Spegazzinia. Based on contrasting morphological features with the extant taxa of Dictyoarthrinium, coupled with the multigene analyses, Dictyoarthrinium musae sp. nov. is introduced herein. Our study provides the first detailed molecular investigation for Dictyoarthrinium and supports its placement in Didymosphaeriaceae (Massarineae, Pleosporales, Dothideomycetes). Previously, Dictyoarthrinium was classified in Apiosporaceae (Xylariales, Sordariomycetes).

HTML

XML

PDF

]]>
Research Article Wed, 5 Aug 2020 08:19:27 +0300
The genus Clavariadelphus (Clavariadelphaceae, Gomphales) in China https://mycokeys.pensoft.net/article/54149/ MycoKeys 70: 89-121

DOI: 10.3897/mycokeys.70.54149

Authors: Hong-Yan Huang, Jie Zhao, Ping Zhang, Zai-Wei Ge, Xian Li, Li-Ping Tang

Abstract: Clavariadelphus species (Clavariadelphaceae, Gomphales) in China were examined using morphology, molecular phylogenetic analyses of ITS data and chemical reactions. Eleven taxa were identified in China, including four species known previously to occur in China (C. griseoclavus, C. ligula, C. sachalinensis and C. yunnanensis), two new record species from China (C. elongatus and C. himalayensis), four novel species (C. alpinus, C. amplus, C. gansuensis and C. khinganensis) and one species that could not be described due to the paucity of material. Finally, we also provided a taxonomic key for the identification of Clavariadelphus species in China.

HTML

XML

PDF

]]>
Research Article Wed, 29 Jul 2020 08:39:25 +0300
New species of Pseudosperma (Agaricales, Inocybaceae) from Pakistan revealed by morphology and multi-locus phylogenetic reconstruction https://mycokeys.pensoft.net/article/33563/ MycoKeys 69: 1-31

DOI: 10.3897/mycokeys.69.33563

Authors: Malka Saba, Danny Haelewaters, Donald H. Pfister, Abdul Nasir Khalid

Abstract: During fungal surveys between 2012 and 2014 in pine-dominated forests of the western Himalayas in Pakistan, several collections of Pseudosperma (Agaricales, Inocybaceae) were made. These were documented, based on morphological and molecular data. During this work, three new species came to light, which are here formally described as Pseudosperma brunneoumbonatum, P. pinophilum and P. triacicularis. These species belong in the genus Pseudosperma fide Matheny et al. (2019) = Pseudosperma clade fide Matheny (2005) = Inocybe sect. Rimosae s.s. fide Larsson et al. (2009). Macro- and micro-morphological descriptions, illustrations and molecular phylogenetic reconstructions of the studied taxa are provided. The new species are differentiated from their close relatives by basidiospore size and colouration of basidiomata. Molecular phylogenetic relationships are inferred using ITS (ITS1–5.8S–ITS2), nrLSU and mtSSU sequence data. All three newly-described taxa likely share an ectomycorrhizal association with trees in the genus Pinus. In addition, five names are recombined in Inosperma, Mallocybe and Pseudosperma. These are Inosperma vinaceobrunneum, Mallocybe erratum, Pseudosperma alboflavellum, Pseudosperma friabile and Pseudosperma neglectum.

HTML

XML

PDF

]]>
Research Article Fri, 10 Jul 2020 15:52:10 +0300
Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture https://mycokeys.pensoft.net/article/50946/ MycoKeys 66: 105-133

DOI: 10.3897/mycokeys.66.50946

Authors: Mohammad Javad Pourmoghaddam, Christopher Lambert, Frank Surup, Seyed Akbar Khodaparast, Irmgard Krisai-Greilhuber, Hermann Voglmayr, Marc Stadler

Abstract: During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coupled with diode array detection and mass spectrometry (HPLC-DAD/MS) and a multi-locus phylogeny based on ITS, LSU, rbp2 and tub2 DNA sequences, revealed a new species here described as Hypoxylon guilanense. In addition, Hypoxylon rubiginosum sensu stricto was also encountered. Concurrently, an endophytic isolate of the latter species showed strong antagonistic activities against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in a dual culture assay in our laboratory. Therefore, we decided to test the new Iranian fungi for antagonistic activities against the pathogen, along with several cultures of other Hypoxylon species that are related to H. rubiginosum. Our results suggest that the antagonistic effects of Hypoxylon spp. against Hym. fraxineus are widespread and that they are due to the production of antifungal phomopsidin derivatives in the presence of the pathogen.

HTML

XML

PDF

]]>
Research Article Fri, 24 Apr 2020 14:27:15 +0300
A taxonomic revision of the genus Conidiobolus (Ancylistaceae, Entomophthorales): four clades including three new genera https://mycokeys.pensoft.net/article/46575/ MycoKeys 66: 55-81

DOI: 10.3897/mycokeys.66.46575

Authors: Yong Nie, De-Shui Yu, Cheng-Fang Wang, Xiao-Yong Liu, Bo Huang

Abstract: The genus Conidiobolus is an important group in entomophthoroid fungi and is considered to be polyphyletic in recent molecular phylogenies. To re-evaluate and delimit this genus, multi-locus phylogenetic analyses were performed using the large and small subunits of nuclear ribosomal DNA (nucLSU and nucSSU), the small subunit of the mitochondrial ribosomal DNA (mtSSU) and the translation elongation factor 1-alpha (EF-1α). The results indicated that the Conidiobolus is not monophyletic, being grouped into a paraphyletic grade with four clades. Consequently, the well-known Conidiobolus is revised and three new genera Capillidium, Microconidiobolus and Neoconidiobolus are proposed along with one new record and 22 new combinations. In addition, the genus Basidiobolus is found to be basal to the other entomophthoroid taxa and the genus Batkoa locates in the Entomophthoraceae clade.

HTML

XML

PDF

]]>
Research Article Mon, 30 Mar 2020 16:27:43 +0300
Ochraceocephala foeniculi gen. et sp. nov., a new pathogen causing crown rot of fennel in Italy https://mycokeys.pensoft.net/article/48389/ MycoKeys 66: 1-22

DOI: 10.3897/mycokeys.66.48389

Authors: Dalia Aiello, Alessandro Vitale, Giancarlo Polizzi, Hermann Voglmayr

Abstract: A new disease of fennel is described from Sicily (southern Italy). Surveys of the disease and sampling were conducted during spring 2017 and 2018 in Adrano and Bronte municipalities (Catania province) where this crop is widely cultivated. Isolations from the margin of symptomatic tissues resulted in fungal colonies with the same morphology. Pathogenicity tests with one isolate of the fungus on 6-month-old plants of fennel reproduced similar symptoms to those observed in nature. Inoculation experiments to assess the susceptibility of six different fennel cultivars to infection by the pathogen showed that the cultivars ‘Narciso’, ‘Apollo’, and ‘Pompeo’ were more susceptible than ‘Aurelio’, ‘Archimede’, and ‘Pegaso’. Phylogenetic analyses based on a matrix of the internal transcribed spacer (ITS), the large subunit (LSU), and the small subunit (SSU) rDNA regions revealed that the isolates represent a new genus and species within the Leptosphaeriaceae, which is here described as Ochraceocephala foeniculi gen. et sp. nov. This study improves the understanding of this new fennel disease, but further studies are needed for planning effective disease management strategies. According to the results of the phylogenetic analyses, Subplenodomus iridicola is transferred to the genus Alloleptosphaeria and Acicuseptoria rumicis to Paraleptosphaeria.

HTML

XML

PDF

]]>
Research Article Mon, 30 Mar 2020 08:36:43 +0300
Diversity of Trametes (Polyporales, Basidiomycota) in tropical Benin and description of new species Trametes parvispora https://mycokeys.pensoft.net/article/47574/ MycoKeys 65: 25-47

DOI: 10.3897/mycokeys.65.47574

Authors: Boris Armel Olou, Franz-Sebastian Krah, Meike Piepenbring, Nourou Soulemane Yorou, Ewald Langer

Abstract: Trametes is a globally distributed genus of white-rot polypores and well sampled in temperate and boreal areas. However, the diversity, taxonomy, and phylogenetic positions of Trametes spp. are poorly known in tropical Africa. This study aims at documenting the diversity of Trametes species in Benin (tropical Africa) and their phylogenetic positions with a focus on the T. elegans species complex. Therefore, we collected specimens of Trametes from different forest types across Benin. To infer phylogenetic relationships between Trametes species, we investigated sequences of five gene regions and added available sequences from GenBank. Using Maximum likelihood and Bayesian phylogeny inference methods, we found eight supported species clades. For the T. elegans species complex, we re-establish the name Trametes palisotii for species previously known as T. elegans in tropical Africa. Furthermore, we propose Trametes parvispora as a species new to science and provide the description of this species. Our molecular phylogeny of Trametes with a focus on tropical Benin contributes to taxonomic clarity of an important wood-decay fungal genus, which is the basis for biodiversity assessments of Trametes in the tropics.

HTML

XML

PDF

]]>
Research Article Tue, 10 Mar 2020 10:12:52 +0200
Arboricolonus simplex gen. et sp. nov. and novelties in Cadophora, Minutiella and Proliferodiscus from Prunus wood in Germany https://mycokeys.pensoft.net/article/46836/ MycoKeys 63: 119-161

DOI: 10.3897/mycokeys.63.46836

Authors: Steffen Bien, Ulrike Damm

Abstract: During a survey on fungi associated with wood necroses of Prunus trees in Germany, strains belonging to the Leotiomycetes and Eurotiomycetes were detected by preliminary analyses of ITS sequences. Multi-locus phylogenetic analyses (LSU, ITS, TUB, EF-1α, depending on genus) of 31 of the 45 strains from Prunus and reference strains revealed several new taxa, including Arboricolonus gen. nov., a new genus in the Helotiales (Leotiomycetes) with a collophorina-like asexual morph. Seven Cadophora species (Helotiales, Leotiomycetes) were treated. The 29 strains from Prunus belonged to five species, of which C. luteo-olivacea and C. novi-eboraci were dominating; C. africana sp. nov., C. prunicola sp. nov. and C. ramosa sp. nov. were revealed as new species. The genus Cadophora was reported from Prunus for the first time. Phialophora bubakii was combined in Cadophora and differentiated from C. obscura, which was resurrected. Asexual morphs of two Proliferodiscus species (Helotiales, Leotiomycetes) were described, including one new species, Pr. ingens sp. nov. Two Minutiella species (Phaeomoniellales, Eurotiomycetes) were detected, including the new species M. pruni-avium sp. nov. Prunus avium and P. domestica are reported as host plants of Minutiella.

HTML

XML

PDF

]]>
Research Article Mon, 2 Mar 2020 16:26:54 +0200
The genus Melanconis (Diaporthales) https://mycokeys.pensoft.net/article/49054/ MycoKeys 63: 69-117

DOI: 10.3897/mycokeys.63.49054

Authors: Walter M. Jaklitsch, Hermann Voglmayr

Abstract: The genus Melanconis (Melanconidaceae, Diaporthales) in the strict sense is here re-evaluated regarding phylogenetic structure, taxonomy, distribution and ecology. Using a matrix of sequences from ITS, LSU, ms204, rpb2, tef1 and tub2, eight species are recognised and their phylogenetic positions are determined. Based on phylogenetic, morphological and geographical differentiation, Melanconis marginalis is subdivided into four subspecies. Melanconis italica is reduced to a subspecies of Melanconis marginalis. The two species Melanconis larissae from Betula sp. and M. pacifica from Alnus rubra are described as new. Melanconis alni and M. stilbostoma are lectotypified and M. alni, M. marginalis and M. stilbostoma are epitypified. All GenBank sequences deposited as Melanconis alni are shown to actually represent M. marginalis and those as M. marginalis belong to the newly described M. pacifica. Currently, Alnus and Betula are the sole host genera of Melanconis. All species and subspecies are (re-)described and illustrated. In addition, the neotypification of Melanconium pterocaryae is here validated.

HTML

XML

PDF

]]>
Research Article Mon, 2 Mar 2020 08:44:12 +0200
Diseases of Cymbopogon citratus (Poaceae) in China: Curvularia nanningensis sp. nov. https://mycokeys.pensoft.net/article/49264/ MycoKeys 63: 49-67

DOI: 10.3897/mycokeys.63.49264

Authors: Qian Zhang, Zai-Fu Yang, Wei Cheng, Nalin N. Wijayawardene, Kevin D. Hyde, Zhuo Chen, Yong Wang

Abstract: Five Curvularia strains isolated from diseased leaves of lemongrass (Cymbopogon citratus) in Guangxi Province, China, were examined. NCBI-Blast searches of ITS sequences suggested a high degree of similarity (99–100%) to Curvularia akaii, C. akaiiensis, C. bothriochloae, C. heteropogonis and C. sichuanensis. To accurately identify these strains, we further analysed their morphology and phylogenetic relationships based on combinations of ITS, GAPDH, and tef1 gene sequences. Morphological observations indicated that the key character differing from similar species was conidial size, whereas phylogenetic analyses indicated that the five strains represent one species that is also distinct from C. akaii, C. akaiiensis and C. bothriochloae by conidial size and conidiophore length. Thus, the strains examined are found to represent a new species described herein as Curvularia nanningensis. The pathogenicity test on the host and detached leaves confirmed the new species to be pathogenic on Cymbopogon citratus leaves. Standardised requirements for reliable identification of Curvularia pathogens are also proposed.

HTML

XML

PDF

]]>
Research Article Thu, 13 Feb 2020 08:30:13 +0200
Longistriata flava (Boletaceae, Basidiomycota) – a new monotypic sequestrate genus and species from Brazilian Atlantic Forest https://mycokeys.pensoft.net/article/39699/ MycoKeys 62: 53-73

DOI: 10.3897/mycokeys.62.39699

Authors: Marcelo A. Sulzbacher, Takamichi Orihara, Tine Grebenc, Felipe Wartchow, Matthew E. Smith, María P. Martín, Admir J. Giachini, Iuri G. Baseia

Abstract: A new monotypic sequestrate genus, Longistriata is described based on collections from the Neotropical forest of Atlantic forest in Paraíba, Northeast Brazil – an area known for its high degree of endemism. The striking features of this new fungus are the hypogeous habit, the vivid yellow peridium in mature basidiomes, broadly ellipsoid basidiospores with a distinct wall that is ornamented with longitudinal striations and lageniform cystidia with rounded apices. Phylogenetic analysis, based on LSU and tef-1α regions, showed that the type species, Longistriata flava, is phylogenetically sister to the monotypic sequestrate African genus Mackintoshia in Boletaceae. Together these two species formed the earliest diverging lineage in the subfamily Zangioideae. Longistriata flava is found in nutrient-poor white sand habitats where plants in the genera Coccoloba (Polygonaceae) and Guapira (Nyctaginaceae) are the only potential ectomycorrhizal host symbionts.

HTML

XML

PDF

]]>
Research Article Mon, 3 Feb 2020 16:39:34 +0200