Latest Articles from MycoKeys Latest 53 Articles from MycoKeys https://mycokeys.pensoft.net/ Thu, 28 Mar 2024 20:56:25 +0200 Pensoft FeedCreator https://mycokeys.pensoft.net/i/logo.jpg Latest Articles from MycoKeys https://mycokeys.pensoft.net/ Corrigendum: Hu H et al. (2023) Taxonomic and phylogenetic characterisations of six species of Pleosporales (in Didymosphaeriaceae, Roussoellaceae and Nigrogranaceae) from China. MycoKeys 100: 123–151. https://doi.org/10.3897/mycokeys.100.109423 https://mycokeys.pensoft.net/article/116896/ MycoKeys 102: 317-322

DOI: 10.3897/mycokeys.102.116896

Authors: Hongmin Hu, Minghui He, Youpeng Wu, Sihan Long, Xu Zhang, Lili Liu, Xiangchun Shen, Nalin N. Wijayawardene, Zebin Meng, Qingde Long, Jichuan Kang, Qirui Li

Abstract: Four new species, Xynobius azonius sp. nov., X. brevifemora sp. nov., X. duoferus sp. nov., and X. stipitoides sp. nov., are described and illustrated, and one species X. geniculatus (Thomson, 1895) is newly reported from South Korea. Xynobius geniculatus (Thomson, 1895) is redescribed and illustrated, and a new combination, Xynobius (Stigmatopoea) cubitalis (Fischer, 1959), comb. nov. is suggested. An identification key to the Xynobius species known from South Korea is provided.

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Corrigenda Thu, 7 Mar 2024 18:54:40 +0200
Two novel species of arctic-alpine lichen-forming fungi (Ascomycota, Megasporaceae) from the Deosai Plains, Pakistan https://mycokeys.pensoft.net/article/113310/ MycoKeys 102: 285-299

DOI: 10.3897/mycokeys.102.113310

Authors: Muhammad Usman, Paul S. Dyer, Matthias Brock, Christopher M. Wade, Abdul Nasir Khalid

Abstract: Members of the lichen-forming fungal genus Oxneriaria are known to occur in cold polar and high altitudinal environments. Two new species, Oxneriaria crittendenii and O. deosaiensis, are now described from the high altitude Deosai Plains, Pakistan, based on phenotypic, multigene phylogenetic and chemical evidence. Phenotypically, O. crittendenii is characterised by orbicular light-brown thalli 1.5–5 cm across, spot tests (K, C, KC) negative, apothecia pruinose, hymenium initially blue then dark orange in response to Lugol’s solution. Oxneriaria deosaiensis is characterised by irregular areolate grey thalli 1.5–2 cm across, K test (light brown), KC test (dark brown), apothecia epruinose, hymenium initially blue then dark blue in response to Lugol’s solution. Both species share the same characters of thalli with black margins and polarilocular ascospores. The closest previously reported species, O. pruinosa, differs from O. crittendenii and O. deosaiensis in having non-lobate margins, thin thalline exciple (45–80 μm thick), short asci (55–80 × 25–42 μm) and K positive (yellow) and KC negative tests and divergent DNA sequence in the ITS, LSU and mt SSU regions. The newly-described Oxneriaria species add to growing evidence of the Deosai Plains as a region of important arctic-alpine biodiversity.

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Research Article Fri, 1 Mar 2024 18:21:56 +0200
Additional four species of Tatraea (Leotiomycetes, Helotiales) in Yunnan Province, China https://mycokeys.pensoft.net/article/112565/ MycoKeys 102: 127-154

DOI: 10.3897/mycokeys.102.112565

Authors: Cui-Jin-Yi Li, Kandawatte Wedaralalage Thilini Chethana, Prapassorn Damrongkool Eungwanichayapant, De-Qun Zhou, Qi Zhao

Abstract: During the investigations of discomycetes in Yunnan, China, five species of Tatraea were discovered on decayed, decorticated oak trees or unidentified wood. All species have typical disc-like, large fruiting bodies with grey, brown or greyish-green colors. The ITS sequence analysis showed that they belong to Tatraea (Helotiaceae, Helotiales) and the LSU and ITS combination revealed a different topology within the genus. Four species, T. clepsydriformis, T. griseoturcoisina, T. yunnanensis and T. yuxiensis were established as new species, and T. aseptata was collected and described on oak woods. The pairwise homoplasy index (PHI) test results indicated that there is no significant genetic recombination (Φw = 1.0) between all related species pairs. All the species described here are supported by descriptions, illustrations and multi-gene analyses.

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Research Article Wed, 14 Feb 2024 12:04:40 +0200
Diversity of Distoseptispora (Distoseptisporaceae) taxa on submerged decaying wood from the Red River in Yunnan, China https://mycokeys.pensoft.net/article/116096/ MycoKeys 102: 1-28

DOI: 10.3897/mycokeys.102.116096

Authors: Hong-Wei Shen, Dan-Feng Bao, Saranyaphat Boonmee, Yong-Zhong Lu, Xi-Jun Su, Yun-Xia Li, Zong-Long Luo

Abstract: The Red River Basin is located in the Indo-Burma biodiversity hotspot and is rich in lignicolous freshwater fungi, but no systematic research has been conducted. A systematic study on the species diversity of lignicolous freshwater fungi in the basin is ongoing. Seven distoseptispora-like specimens were collected from the Red River Basin in Yunnan. Phylogenetic analysis of ITS, LSU, tef1-α, and rpb2 genes and combined morphological data indicate that there are six distinct species of Distoseptispora, including two new species and four known species. Two new species were named D. suae and D. xinpingensis, and the four known species were D. bambusae, D. euseptata, D. obpyriformis and D. pachyconidia. This study provides detailed descriptions and illustrations of these six species and an updated phylogenetic backbone tree of Distoseptispora.

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Research Article Mon, 5 Feb 2024 10:43:53 +0200
Two novel freshwater hyphomycetes, in Acrogenospora (Minutisphaerales, Dothideomycetes) and Conioscypha (Conioscyphales, Sordariomycetes) from Southwestern China https://mycokeys.pensoft.net/article/115209/ MycoKeys 101: 249-273

DOI: 10.3897/mycokeys.101.115209

Authors: Lu Li, Hong-Zhi Du, Vinodhini Thiyagaraja, Darbhe Jayarama Bhat, Rungtiwa Phookamsak, Ratchadawan Cheewangkoon

Abstract: Freshwater fungi are highly diverse in China and frequently reported from submerged wood, freshwater insects, herbaceous substrates, sediments, leaves, foams, and living plants. In this study, we investigated two freshwater species that were collected from Yunnan and Guizhou provinces in China. Detailed morphological analysis complemented by multi-gene phylogenetic analyses based on LSU, SSU, ITS, RPB2 and TEF1-α sequences data revealed them to be two new saprobic species, namely Acrogenospora alangii sp. nov. and Conioscypha yunnanensis sp. nov. in their asexual morphs. Additionally, Acrogenospora alangii sp. nov. is reported for the first time as a freshwater ascomycete associated with the medicinal plant Alangium chinense (Alangiaceae). Detailed morphological descriptions, illustrations and updated phylogenetic relationships of the new taxa are provided herein.

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Research Article Wed, 31 Jan 2024 11:54:26 +0200
Species diversity and major host/substrate associations of the genus Akanthomyces (Hypocreales, Cordycipitaceae) https://mycokeys.pensoft.net/article/109751/ MycoKeys 101: 113-141

DOI: 10.3897/mycokeys.101.109751

Authors: Yao Wang, Zhi-Qin Wang, Run Luo, Sisommay Souvanhnachit, Chinnapan Thanarut, Van-Minh Dao, Hong Yu

Abstract: Akanthomyces, a group of fungi with rich morphological and ecological diversity in Cordycipitaceae (Ascomycota, Hypocreales), has a wide distribution amongst diverse habitats. By surveying arthropod-pathogenic fungi in China and Southeast Asia over the last six years, nine Akanthomyces spp. were found and identified. Five of these were shown to represent four known species and an undetermined species of Akanthomyces. Four of these were new species and they were named A. kunmingensis and A. subaraneicola from China, A. laosensis from Laos and A. pseudonoctuidarum from Thailand. The new species were described and illustrated according to the morphological characteristics and molecular data. Akanthomyces araneogenus, which was isolated from spiders from different regions in China, Thailand and Vietnam, was described as a newly-recorded species from Thailand and Vietnam. The phylogenetic positions of the nine species were evaluated, based on phylogenetic inferences according to five loci, namely, ITS, nrLSU, TEF, RPB1 and RPB2. In this study, we reviewed the research progress achieved for Akanthomyces regarding its taxonomy, species diversity, geographic distribution and major host/substrate associations. The morphological characteristics of 35 species in Akanthomyces, including four novel species and 31 known taxa, were also compared.

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Research Article Mon, 15 Jan 2024 10:35:59 +0200
Identification and fungicide sensitivity of Microdochium chrysopogonis (Ascomycota, Amphisphaeriaceae), a new species causing tar spot of Chrysopogon zizanioides in southern China https://mycokeys.pensoft.net/article/112128/ MycoKeys 100: 205-232

DOI: 10.3897/mycokeys.100.112128

Authors: Xiang Lu, Mengxian Mai, Wenhui Tan, Muyan Zhang, Jie Xie, Yi Lu, Xue Li Niu, Wu Zhang

Abstract: Vetiver grass (Chrysopogon zizanioides) has received extensive attention in recent years due to its diverse applications in soil and water conservation, heavy metal remediation, as well as essential oil and phenolic acids extraction. In 2019, the emergence of tar spot disease on C. zizanioides was documented in Zhanjiang, Guangdong Province, China. Initially, the disease manifested as black ascomata embedded within leaf tissue, either scattered or clustered on leaf surfaces. Subsequently, these ascomata became surrounded by fisheye lesions, characterised by brown, elliptical, necrotic haloes, which eventually coalesced, resulting in leaf withering. Koch’s postulates demonstrated that the fungus isolated from these lesions was the causal agent. Microscopic examination showed that the pathogen morphologically belonged to Microdochium. The phylogenetic tree inferred from the combined ITS, LSU, tub2 and rpb2 sequences revealed the three isolates including GDMCC 3.683, LNU-196 and LNU-197 to be a novel species of Microdochium. Combining the results of phylogenetic, pathogenicity and morphological analyses, we propose a new species named M. chrysopogonis as the causal agent of C. zizanioides in southern China. The optimum growth temperature for M. chrysopogonis was determined to be 30 °C. The in vitro fungicide sensitivity of M. chrysopogonis was determined using a mycelial growth assay. Four demethylation-inhibiting (DMI) fungicides, including difenoconazole, flusilazole, propiconazole and tebuconazole and one methyl benzimidazole carbamate (MBC) fungicide, carbendazim, were effective against M. chrysopogonis, with mean 50% effective concentration (EC50) values of 0.077, 0.011, 0.004, 0.024 and 0.007 μg/ml, respectively. These findings provide essential references for the precise diagnosis and effective management of M. chrysopogonis.

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Research Article Wed, 6 Dec 2023 14:43:01 +0200
Morphology, phylogeny and host specificity of two new Ophiocordyceps species belonging to the “zombie-ant fungi” clade (Ophiocordycipitaceae, Hypocreales) https://mycokeys.pensoft.net/article/107565/ MycoKeys 99: 269-296

DOI: 10.3897/mycokeys.99.107565

Authors: Dexiang Tang, Jing Zhao, Yingling Lu, Zhiqin Wang, Tao Sun, Zuoheng Liu, Hong Yu

Abstract: Species of the genus Ophiocordyceps, which include species able to manipulate the behaviour of ants, are known as the “zombie-ant fungi” and have attracted much attention over the last decade. They are widespread within tropical, subtropical and even temperate forests worldwide, with relatively few reports from subtropical monsoon evergreen broad-leaved forest. Fungal specimens have been collected from China, occurring on ants and producing hirsutella-like anamorphs. Based on a combination of morphological characters, phylogenetic analyses (LSU, SSU, TEF1a, RPB1 and RPB2) and ecological data, two new species, Ophiocordyceps tortuosa and O. ansiformis, are identified and proposed herein. Ophiocordyceps tortuosa and O. ansiformis are recorded on the same species of Colobopsis ant, based on phylogenetic analyses (COI), which may be sharing the same host. Ophiocordyceps tortuosa and O. ansiformis share the morphological character of producing lanceolate ascospores. They have typical characteristics distinguished from other species. The ascospore of O. tortuosa are tortuously arranged in the ascus and the ascospore of O. ansiformis have a structure like a handle-shape in the middle. Our molecular data also indicate that O. tortuosa and O. ansiformis are clearly distinct from other species.

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Research Article Mon, 16 Oct 2023 18:22:52 +0300
Three coralloid species of the genus Trechispora (Trechisporales, Basidiomycota) in China: two newly discovered taxa and one reported for the first time https://mycokeys.pensoft.net/article/109375/ MycoKeys 99: 153-170

DOI: 10.3897/mycokeys.99.109375

Authors: Peng-Tao Deng, Jun Yan, Xiang-Fen Liu, Zheng-Mi He, Yuan Lin, Ming-Xin Lu, Ping Zhang

Abstract: Two new species of Trechispora indigenous to southern China, T. laxa and T. tongdaoensis, are described and illustrated, and the first record of T. khokpasiensis in China is reported. Molecular phylogenetic analyses of the concatenated nuclear rDNA ITS1–5.8S–ITS2 and nuclear large subunit sequences supported the inclusion of the three species within the Trechispora clade, together with species formerly classified in Scytinopogon. The new species are similar in micromorphology to species of Trechispora (as traditionally circumscribed) but are distinguished by having coralloid basidiomata. A key to the known coralloid Trechispora species in China is provided.

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Research Article Thu, 7 Sep 2023 12:40:21 +0300
Morphological and phylogenetic analyses reveal three new species and one new record of Tubeufia (Tubeufiales, Tubeufiaceae) from southern China https://mycokeys.pensoft.net/article/107606/ MycoKeys 99: 87-108

DOI: 10.3897/mycokeys.99.107606

Authors: Jian Ma, Li-Juan Zhang, Saranyaphat Boonmee, Xing-Juan Xiao, Ning-Guo Liu, Yuan-Pin Xiao, Zong-Long Luo, Yong-Zhong Lu

Abstract: During an investigation of helicosporous fungi in China, a total of seven helicosporous hyphomycetes were obtained from decaying wood in the southern region of the country. Based on phylogenetic analyses using a combined LSU, ITS, tef1α, and rpb2 sequence matrix, in conjunction with morphological comparisons, these taxa were classified within Tubeufia (Tubeufiaceae, Tubeufiales) and were recognized as three new species, viz. Tubeufia guttulata, T. hainanensis, and T. muriformis, as well as one new distribution record, viz. T. cocois. Evidence for these new taxa and the new record, descriptions, illustrations, notes, and phylogenetic evidence are provided for the newly collected helicosporous species.

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Research Article Mon, 14 Aug 2023 17:50:32 +0300
The phylogeny and taxonomy of Glypholecia (Acarosporaceae, lichenized Ascomycota), including a new species from northwestern China https://mycokeys.pensoft.net/article/104314/ MycoKeys 98: 153-165

DOI: 10.3897/mycokeys.98.104314

Authors: An-cheng Yin, Qiu-yi Zhong, Christoph Scheidegger, Ji-zhen Jin, Fiona R. Worthy, Li-song Wang, Xin-yu Wang

Abstract: Glypholecia qinghaiensis An C. Yin, Q. Y. Zhong & Li S. Wang is described as new to science. It is characterized by its squamulose thallus, compound apothecia, ellipsoid ascospores, and the presence of rhizines on the lower surface of the thallus. A phylogenetic tree of Glypholecia species was constructed based on nrITS and mtSSU sequences. Two species G. qinghaiensis and G. scabra are confirmed in China.

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Research Article Wed, 21 Jun 2023 10:12:07 +0300
Morphological and molecular analyses reveal two new species of Microcera (Nectriaceae, Hypocreales) associated with scale insects on walnut in China https://mycokeys.pensoft.net/article/103484/ MycoKeys 98: 19-35

DOI: 10.3897/mycokeys.98.103484

Authors: Feng Liu, Yu Deng, Fei-Hu Wang, Rajesh Jeewon, Qian Zeng, Xiu-Lan Xu, Ying-Gao Liu, Chun-Lin Yang

Abstract: The fungal genus Microcera consists of species mostly occurring as parasites of scale insects, but are also commonly isolated from soil or lichens. In the present study, we surveyed the diversity and assess the taxonomy of entomopathogenic fungi in Sichuan Province, China. Two new species of Microcera, viz. M. chrysomphaludis and M. pseudaulacaspidis, were isolated from scale insects colonising walnut (Juglans regia). Maximum Likelihood and Bayesian Inference analyses of ITS, LSU, tef1-α, rpb1, rpb2, acl1, act, tub2, cmdA and his3 sequence data provide evidence for the validity of the two species and their placement in Nectriaceae (Hypocreales). Microcera pseudaulacaspidis primarily differs from similar species by having more septate and smaller cylindrical macroconidia, as well as DNA sequence data. Meanwhile, Microcera chrysomphaludis has elliptical, one-septate ascospores with acute ends and cylindrical, slightly curved with 4–6 septate macroconidia up to 78 µm long. Morphological descriptions with illustrations of the novel species and DNA-based phylogeny generated from analyses of multigene dataset are also provided to better understand species relationships.

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Research Article Mon, 29 May 2023 17:55:50 +0300
Botryosphaerialean fungi associated with woody oil plants cultivated in Sichuan Province, China https://mycokeys.pensoft.net/article/103118/ MycoKeys 97: 71-116

DOI: 10.3897/mycokeys.97.103118

Authors: Wen-Li Li, Rui-Ru Liang, Asha J. Dissanayake, Jian-Kui Liu

Abstract: Woody oil plants are important economic trees which are widely cultivated and distributed throughout China. Surveys conducted during 2020 and 2021 on several woody oil plantations from five regions of Sichuan Province, China, revealed a high diversity of Botryosphaerialean fungi. The identification of 50 botryosphaeriaceous isolates was carried out based on both morphology and multi-gene phylogenetic analysis of internal transcribed spacer region (ITS), translation elongation factor 1-alpha gene (tef1) and β-tubulin gene (tub2). This allowed the identification of twelve previously known Botryosphaeriales species: Aplosporella prunicola, A. ginkgonis, Barriopsis tectonae, Botryosphaeria dothidea, Bo. fabicerciana, Diplodia mutila, Di. seriata, Dothiorella sarmentorum, Neofusicoccum parvum, Sardiniella guizhouensis, Sphaeropsis citrigena, and Sp. guizhouensis, and four novel species belonging to the genera Diplodia and Dothiorella, viz. Di. acerigena, Di. pistaciicola, Do. camelliae and Do. zanthoxyli. The dominant species isolated across the surveyed regions were Botryosphaeria dothidea, Sardiniella guizhouensis and Diplodia mutila, representing 20%, 14% and 12% of the total isolates, respectively. In addition, most isolates were obtained from Pistacia chinensis (14 isolates), followed by Camellia oleifera (10 isolates). The present study enhances the understanding of Botryosphaeriales species diversity on woody oil plants in Sichuan Province, China.

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Research Article Tue, 23 May 2023 14:38:16 +0300
Three new species of Trichoderma (Hypocreales, Hypocreaceae) from soils in China https://mycokeys.pensoft.net/article/101635/ MycoKeys 97: 21-40

DOI: 10.3897/mycokeys.97.101635

Authors: Rui Zhao, Li-Juan Mao, Chu-Long Zhang

Abstract: Trichoderma spp. are diverse fungi with wide distribution. In this study, we report on three new species of Trichoderma, namely T. nigricans, T. densissimum and T. paradensissimum, collected from soils in China. Their phylogenetic position of these novel species was determined by analyzing the concatenated sequences of the second largest nuclear RNA polymerase subunit encoding gene (rpb2) and the translation elongation factor 1– alpha encoding gene (tef1). The results of the phylogenetic analysis showed that each new species formed a distinct clade: T. nigricans is a new member of the Atroviride Clade, and T. densissimum and T. paradensissimum belong to the Harzianum Clade. A detailed description of the morphology and cultural characteristics of the newly discovered Trichoderma species is provided, and these characteristics were compared with those of closely related species to better understand the taxonomic relationships within the Trichoderma.

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Research Article Tue, 2 May 2023 11:44:55 +0300
Endophytic Colletotrichum (Sordariomycetes, Glomerellaceae) species associated with Citrus grandis cv. “Tomentosa” in China https://mycokeys.pensoft.net/article/87121/ MycoKeys 95: 163-188

DOI: 10.3897/mycokeys.95.87121

Authors: Jia-Wei Liu, Ishara S. Manawasinghe, Xuan-Ni Liao, Jin Mao, Zhang-Yong Dong, Ruvishika S. Jayawardena, Dhanushka N. Wanasinghe, Yong-Xin Shu, Mei Luo

Abstract: Colletotrichum species are well-known plant pathogens, saprobes, endophytes, human pathogens and entomopathogens. However, little is known about Colletotrichum as endophytes of plants and cultivars including Citrus grandis cv. “Tomentosa”. In the present study, 12 endophytic Colletotrichum isolates were obtained from this host in Huazhou, Guangdong Province (China) in 2019. Based on morphology and combined multigene phylogeny [nuclear ribosomal internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (gapdh), chitin synthase 1 (chs-1), histone H3 (his3) actin (act), beta-tubulin (β-tubulin) and glutamine synthetase (gs)], six Colletotrichum species were identified, including two new species, namely Colletotrichum guangdongense and C. tomentosae. Colletotrichum asianum, C. plurivorum, C. siamense and C. tainanense are identified as being the first reports on C. grandis cv. “Tomentosa” worldwide. This study is the first comprehensive study on endophytic Colletotrichum species on C. grandis cv. “Tomentosa” in China.

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Research Article Thu, 23 Feb 2023 18:14:23 +0200
Iugisporipsathyra reticulopilea gen. et sp. nov. (Agaricales, Psathyrellaceae) from tropical China produces unique ridge-ornamented spores with an obvious suprahilar plage https://mycokeys.pensoft.net/article/85690/ MycoKeys 90: 147-162

DOI: 10.3897/mycokeys.90.85690

Authors: Sheng-Nan Wang, Yu-Guang Fan, Jun-Qing Yan

Abstract: Iugisporipsathyra, a new psathyrelloid genus from tropical red soil of China, is established with I. reticulopilea as the type species. The new genus is characterised by basidiomata psathyrelloid, pileus rugose to appearing reticulate ridged, covered by persistent, but inconspicuous villus, pleurocystidia absent and ridge-ornamented spores with an obvious suprahilar plage. The genus is unique amongst Psathyrellaceae in producing ridge-ornamented spores with an obvious suprahilar plage and forms a distinct lineage within Psathyrellaceae, based on the Maximum Likelihood and Bayesian Inference analyses of a combined three-gene sequence dataset (ITS, LSU and β-tub). Full descriptions and photographs of the new genus and species are presented.

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Research Article Wed, 22 Jun 2022 16:34:05 +0300
Dendrocorticiopsis orientalis gen. et sp. nov. of the Punctulariaceae (Corticiales, Basidiomycota) revealed by molecular data https://mycokeys.pensoft.net/article/84562/ MycoKeys 90: 19-30

DOI: 10.3897/mycokeys.90.84562

Authors: Chia-Ling Wei, Che-Chih Chen, Shuang-Hui He, Sheng-Hua Wu

Abstract: Dendrocorticiopsis orientalis is presented in this study as a new genus and new species based on morphological and phylogenetic evidence. This new taxon is characterized by resupinate, smooth and membranaceous basidiomata, monomitic hyphal system with clamps, colorless dendrohyphidia, variable presence of cystidia, and ellipsoid to ovoid basidiospores measuring 5–7 × 3.2–5.2 μm. The phylogenetic analyses based on the ITS1-5.8S-ITS2 (ITS) + nuclear 28S rDNA (28S) dataset of Corticiales indicated that the new taxon is nested in Punctulariaceae, separated from other genera with strong support values. Descriptions, specimen photo, and illustrations of the new taxon are provided in this study. A morphological comparison of the four genera of Punctulariaceae is given.

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Research Article Tue, 31 May 2022 09:45:56 +0300
Three new species of Candolleomyces (Agaricomycetes, Agaricales, Psathyrellaceae) from the Yanshan Mountains in China https://mycokeys.pensoft.net/article/81437/ MycoKeys 88: 109-121

DOI: 10.3897/mycokeys.88.81437

Authors: Hao Zhou, GuiQiang Cheng, XiMei Sun, RuiYi Cheng, HongLiang Zhang, YanMin Dong, ChengLin Hou

Abstract: Three new species, Candolleomyces incanus, C. subcandolleanus and C. yanshanensis, were found and described from Yanshan Mountains in China. The identification is based on morphological observation combined with phylogenetic analysis of ITS-LSU-Tef1α-TUB2. This study enriched the species diversity of Candolleomyces in Yanshan Mountains and provided important data support for the systematic study of Candolleomyces in the future.

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Research Article Wed, 13 Apr 2022 14:54:13 +0300
Revision of Immersaria and a new lecanorine genus in Lecideaceae (lichenised Ascomycota, Lecanoromycetes) https://mycokeys.pensoft.net/article/72614/ MycoKeys 87: 99-132

DOI: 10.3897/mycokeys.87.72614

Authors: Cong-Miao Xie, Li-Song Wang, Zun-Tian Zhao, Yan-Yun Zhang, Xin-Yu Wang, Lu-Lu Zhang

Abstract: The species Immersaria cupreoatra has been included in Bellemerea. This caused us to reconsider the relationships between Bellemerea and the lecanorine species of Immersaria and to question the monophyly of Immersaria. Amongst 25 genera of the family Lecideaceae, most have lecideine apothecia, the exceptions being Bellemerea and Koerberiella, which have lecanorine apothecia. According to previous classifications, Immersaria included species with both lecanorine and lecideine apothecia. A five-loci phylogenetic tree (nrITS, nrLSU, RPB1, RPB2, and mtSSU) for Lecideaceae showed that Immersaria was split into two clades: firstly, all the lecideine apotheciate species and secondly, all the lecanorine apotheciate species. The latter clade was closely related to the remaining lecanorine apotheciate genera: Bellemerea and Koerberiella. Therefore, the genus concept of Immersaria is revised accordingly and a new lecanorine genus Lecaimmeria is proposed. Furthermore, four new species for Immersaria and seven new species and three new combinations for the new genus Lecaimmeria are proposed. Keys to Immersaria and the new genus Lecaimmeria are provided.

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Research Article Tue, 15 Feb 2022 11:09:19 +0200
Two new species of Phallus (Phallaceae) with a white indusium from China https://mycokeys.pensoft.net/article/75309/ MycoKeys 85: 109-125

DOI: 10.3897/mycokeys.85.75309

Authors: Ting Li, Wang-Qiu Deng, Bin Song, Ming Zhang, Mu Wang, Tai-Hui Li

Abstract: Two new Phallus species, P. cremeo-ochraceus and P. rigidiindusiatus were discovered in southwestern and southern China, respectively. Phallus cremeo-ochraceus is morphologically characterized by its cream to ochraceous receptacle, white to very slightly pinkish indusium, white to pinkish pseudostipe and white to slightly purplish pink volva. Phallus rigidiindusiatus is characterized by a white to yellowish white receptacle, a strongly rigid indusium usually without serrated margin and smaller basidiospores than those of P. serratus. Phylogenetic positions of the two species are located in two independent lineages respectively. Detailed descriptions, color photographs, illustrations and a key to the related species are presented.

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Research Article Thu, 16 Dec 2021 10:53:45 +0200
Two novel species and two new records of Distoseptispora from freshwater habitats in China and Thailand https://mycokeys.pensoft.net/article/71905/ MycoKeys 84: 79-101

DOI: 10.3897/mycokeys.84.71905

Authors: Hong-Wei Shen, Dan-Feng Bao, Kevin D. Hyde, Hong-Yan Su, Darbhe J. Bhat, Zong-Long Luo

Abstract: During investigations into freshwater fungi from the Great Mekong Subregion, four Distoseptispora taxa were collected from China and Thailand. Based on morphological characteristics, and phylogenetic analyses of combined LSU, ITS, SSU, TEF1-α, and RPB2 sequence data, two new species Distoseptispora bangkokensis and D. lancangjiangensis are introduced, and two known species D. clematidis and D. thysanolaenae were first reported in freshwater habitat. Illustrations and descriptions of these taxa are provided, along with comparisons with extant taxa in the genus.

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Research Article Mon, 8 Nov 2021 19:49:36 +0200
Three new Russula species in sect. Ingratae (Russulales, Basidiomycota) from southern China https://mycokeys.pensoft.net/article/68750/ MycoKeys 84: 103-139

DOI: 10.3897/mycokeys.84.68750

Authors: Guo-Jie Li, Shou-Mian Li, Bart Buyck, Shi-Yi Zhao, Xue-Jiao Xie, Lu-Yao Shi, Chun-Ying Deng, Qing-Feng Meng, Qi-Biao Sun, Jun-Qing Yan, Jing Wang, Ming Li

Abstract: Three new species of Russulasection Ingratae, found in Guizhou and Jiangsu Provinces, southern China, are proposed: R. straminella, R. subpectinatoides and R. succinea. Photographs, line drawings and detailed morphological descriptions for these species are provided with comparisons against closely-related taxa. Phylogenetic analysis of the internal transcribed spacer (ITS) region supported the recognition of these specimens as new species. Additionally, R. indocatillus is reported for the first time from China and morphological and phylogenetic data are provided for the Chinese specimens.

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Research Article Mon, 8 Nov 2021 12:56:59 +0200
Characterization of Diaporthe species on Camellia oleifera in Hunan Province, with descriptions of two new species https://mycokeys.pensoft.net/article/71701/ MycoKeys 84: 15-33

DOI: 10.3897/mycokeys.84.71701

Authors: Qin Yang, Jie Tang, Guo Y. Zhou

Abstract: Tea-oil tree (Camellia oleifera Abel.) is an important edible oil woody plant with a planting area over 3,800,000 hectares in southern China. Species of Diaporthe inhabit a wide range of plant hosts as plant pathogens, endophytes and saprobes. At present, relatively little is known about the taxonomy and genetic diversity of Diaporthe on C. oleifera. Here, we conducted an extensive field survey in Hunan Province in China to identify and characterise Diaporthe species associated with tea-oil leaf spots. As a result, eleven isolates of Diaporthe were obtained from symptomatic C. oleifera leaves. These isolates were studied by applying a polyphasic approach including morphological and phylogenetic analyses of partial ITS, cal, his3, tef1 and tub2 gene regions. Two new Diaporthe species (D. camelliae-oleiferae and D. hunanensis) were proposed and described herein, and C. oleifera was revealed to be new host records of D. hubeiensis and D. sojae. This study indicated there is a potential of more undiscovered Diaporthe species from C. oleifera in China.

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Research Article Mon, 18 Oct 2021 11:45:37 +0300
Morphology and molecular study of three new Cordycipitoid fungi and its related species collected from Jilin Province, northeast China https://mycokeys.pensoft.net/article/72325/ MycoKeys 83: 161-180

DOI: 10.3897/mycokeys.83.72325

Authors: Jia-Jun Hu, Gui-Ping Zhao, Yong-Lan Tuo, Dan Dai, Di-Zhe Guo, Gu Rao, Zheng-Xiang Qi, Zhen-Hao Zhang, Yu Li, Bo Zhang

Abstract: Cordyceps species are notable medicinal fungi in China, which are pathogenic on insects and exhibit high biodiversity in tropical and subtropical regions. Recently, three new Cordyceps species, Cordyceps changchunensis and Cordyceps jingyuetanensis growing on pupae of Lepidoptera and Cordyceps changbaiensis growing on larvae of Lepidoptera, were found in Jilin Province, China and are described, based on morphological and ecological characteristics. These three new species are similar to the Cordyceps militaris group, but are distinctly distinguishable from the known species. Cordyceps changchunensis, characterised by its small and light yellow to orange stromata which is occasionally forked, covered with white mycelium at the base of stipe, globose to ovoid perithecia, is macroscopically similar to Cordyceps militaris. Cordyceps changbaiensis is clearly discriminated from other Cordyceps species by its white to orange and branched stromata, clavate to cylindrical fertile apical portion, immersed and globose to ovoid perithecia. Moreover, unbranched, clavate and orange to light red stromata, almond-shaped to ovoid and immersed perithecia separate Cordyceps jingyuetanensis from other Cordyceps species. nrITS, nrLSU and EF-1α sequences were undertaken and phylogenetic trees, based on Maximum Likelihood and Bayesian Inference analysis showed that the three new species clustered with Cordyceps militaris, but formed individual clades, as well as confirmed the results of our morphological study.

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Research Article Mon, 27 Sep 2021 17:57:48 +0300
Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota) https://mycokeys.pensoft.net/article/63241/ MycoKeys 82: 33-56

DOI: 10.3897/mycokeys.82.63241

Authors: Long-Fei Fan, Renato Lúcio Mendes Alvarenga, Tatiana Baptista Gibertoni, Fang Wu, Yu-Cheng Dai

Abstract: Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species (T. cheejenii, T. fibulifera s.s., T. “neofibulifera”, T. lloydiae-candidae and T. olens) and four new species (T. australe, T. guangxiensis, T. latispora and T. subfibulifera) in the complex are recognized based on morphological characteristics, molecular evidence, and geographic distribution. Sequences of eight species of the complex were included in the phylogenetic analyses because T. olens lacks molecular data. The phylogenetic analyses were performed by a combined sequence dataset of the internal transcribed spacer (ITS) and the partial nuclear large subunit rDNA (nLSU), and a combined sequence dataset of the ITS, partial nLSU, the small subunit mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α (TEF1), the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2). The eight species formed eight independent lineages with robust support in phylogenies based on both datasets. Illustrated description of the six species including Tremella fibulifera s.s., T. “neofibulifera” and four new species, and discussions with their related species, are provided. A table of the comparison of the important characteristics of nine species in the T. fibulifera complex and a key to the whitish species in Tremella s.s. are provided.

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Research Article Wed, 4 Aug 2021 15:09:13 +0300
Cyanescent Gyroporus (Gyroporaceae, Boletales) from China https://mycokeys.pensoft.net/article/65660/ MycoKeys 81: 165-183

DOI: 10.3897/mycokeys.81.65660

Authors: Cong Huang, Ming Zhang, Xing-Liang Wu, Gang Wu, Jian-Ping Xu, Zhu L. Yang, Yan-Chun Li

Abstract: Gyroporus species with cyanescent oxidation reactions were investigated, based on morphology and phylogenetic analysis of DNA sequences from the nuclear ribosomal large subunit (nrLSU), the nuclear ribosomal internal transcribed spacer (ITS) and the mitochondrial adenosine triphosphate ATP synthase subunit 6 (atp6). Three species, including two new species, namely G. alpinus and G. flavocyanescens and one previously-described species, namely G. brunneofloccosus, are revealed from China. Collections formerly reported from China as “G. cyanescens” are either G. alpinus or G. flavocyanescens. The new species are documented and illustrated in detail, while the concept of G. brunneofloccosus is refined with additional recently-collected materials. Additionally, the cyanescent species G. pseudomicrosporus, previously described from China, is shown to be a member of the genus Gyrodon, based on re-examination of the type specimen. A key to the cyanescent Gyroporus species from China is provided.

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Research Article Mon, 26 Jul 2021 09:44:46 +0300
Two new rare species of Candolleomyces with pale spores from China https://mycokeys.pensoft.net/article/67166/ MycoKeys 80: 149-161

DOI: 10.3897/mycokeys.80.67166

Authors: Tolgor Bau, Jun-Qing Yan

Abstract: Most species of Candolleomyces have brown or dark brown spores. Although pale-spored members are rare in the genus we frequently collected two such species from many Provinces during our investigations in subtropical China from 2016–2020. As revealed by morphological characterisation and multigene phylogenetic analyses (ITS LSU β-tub and tef-1α) these species which we have named C. subcacao and C. subminutisporus are unique and distinct from known taxa. In addition a new combination C. cladii-marisci is proposed on the basis of ITS sequence analysis of the type specimen. Detailed descriptions colour photos illustrations and a key to related species are presented.

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Research Article Thu, 3 Jun 2021 13:43:51 +0300
Characterization of Diaporthe species associated with peach constriction canker, with two novel species from China https://mycokeys.pensoft.net/article/63816/ MycoKeys 80: 77-90

DOI: 10.3897/mycokeys.80.63816

Authors: Xianhong Wang, Yashuang Guo, Yamin Du, Ziling Yang, Xinzhong Huang, Ni Hong, Wenxing Xu, Guoping Wang

Abstract: Species of Diaporthe infect a wide range of plants and live in vivo as endophytes, saprobes or pathogens. However, those in peach plants are poorly characterized. In this study, 52 Diaporthe strains were isolated from peach branches with buds, showing constriction canker symptoms. Phylogenetic analyses were conducted using five gene regions: internal transcribed spacer of the ribosomal DNA (ITS), translation elongation factor 1-α (TEF), ß-tubulin (TUB), histone (HIS), and calmodulin (CAL). These results coupled with morphology revealed seven species of Diaporthe, including five known species (D. caryae, D. cercidis, D. eres, D. hongkongensis, and D. unshiuensis). In addition, two novel species D. jinxiu and D. zaofenghuang are introduced. Except for the previously reported D. eres, this study represents the first characterization of Diaporthe species associated with peach constriction canker in China, and contributes useful data for practicable disease management.

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Research Article Tue, 18 May 2021 08:11:22 +0300
Diversity of Plectosphaerella within aquatic plants from southwest China, with P. endophytica and P. sichuanensis spp. nov. https://mycokeys.pensoft.net/article/64624/ MycoKeys 80: 57-75

DOI: 10.3897/mycokeys.80.64624

Authors: Xiao Qian Yang, Shi Yun Ma, Ze Xiang Peng, Zhong Qiao Wang, Min Qiao, Zefen Yu

Abstract: Members of Plectosphaerella inhabit different substrates, including plants, soil and insects, and most species are pathogens causing large losses in agriculture. During a survey of endophytic fungi in aquatic plants in southwest China, 112 strains of Plectosphaerella were isolated, representing two new species, P. endophytica sp. nov. and P. sichuanensis sp. nov., as well as two known species, P. cucumerina and P. pauciseptata. The novel taxa are described and illustrated here using combined morphological and multi-locus phylogenetic (LSU-ITS-TEF-1α-TUB2) analyses. Our result revealed Plectosphaerella species inhabiting within aquatic plants in southwest China, and the separation frequency of each species was presented.

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Research Article Tue, 11 May 2021 15:31:59 +0300
The insights into the evolutionary history of Translucidithyrium: based on a newly-discovered species https://mycokeys.pensoft.net/article/58628/ MycoKeys 76: 1-16

DOI: 10.3897/mycokeys.76.58628

Authors: Xinhao Li, Hai-Xia Wu, Jinchen Li, Hang Chen, Wei Wang

Abstract: During the field studies, a Translucidithyrium-like taxon was collected in Xishuangbanna of Yunnan Province, during an investigation into the diversity of microfungi in the southwest of China. Morphological observations and phylogenetic analysis of combined LSU and ITS sequences revealed that the new taxon is a member of the genus Translucidithyrium and it is distinct from other species. Therefore, Translucidithyrium chinense sp. nov. is introduced here. The Maximum Clade Credibility (MCC) tree from LSU rDNA of Translucidithyrium and related species indicated the divergence time of existing and new species of Translucidithyrium was crown age at 16 (4–33) Mya. Combining the estimated divergence time, paleoecology and plate tectonic movements with the corresponding geological time scale, we proposed a hypothesis that the speciation (estimated divergence time) of T. chinense was earlier than T. thailandicum. Our findings provided new insights into the species of Translucidithyrium about ecological adaptation and speciation in two separate areas.

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Research Article Thu, 17 Dec 2020 08:48:31 +0200
Three new species from Guangdong Province of China, and a molecular assessment of Hygrocybe subsection Hygrocybe https://mycokeys.pensoft.net/article/59600/ MycoKeys 75: 145-161

DOI: 10.3897/mycokeys.75.59600

Authors: Chao-Qun Wang, Ming Zhang, Tai-Hui Li

Abstract: Blackening waxcaps (Hygrocybe subsect. Hygrocybe) are a group of colorful and attractive mushrooms. However, the species diversity of subsect. Hygrocybe in China is still poorly known due to the limited sampling. In this study, three new species of this group from Guangdong Province, China are described and illustrated based on their morphological characteristics and molecular analyses of the internal transcribed spacer and large subunit ribosomal DNA regions. Hygrocybe debilipes from grasslands of South China Sea islands is mainly characterized by its orange red to vivid red pileus, fragile stipe, and ellipsoid to oblong basidiospores; H. griseonigricans from woodlands is characterized by its whitish to dull yellow pileus, quick black discoloration and the globose, subglobose to broadly ovoid basidiospores; H. rubroconica from woodlands is characterized by the hemispheric to plano-convex pileus when mature, semitranslucent fibrose stipe, and globose to ellipsoid basidiospores.

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Research Article Wed, 9 Dec 2020 14:08:06 +0200
Three new species of Cortinarius subgenus Telamonia (Cortinariaceae, Agaricales) from China https://mycokeys.pensoft.net/article/49437/ MycoKeys 69: 91-109

DOI: 10.3897/mycokeys.69.49437

Authors: Meng-Le Xie, Tie-Zheng Wei, Yong-Ping Fu, Dan Li, Liang-Liang Qi, Peng-Jie Xing, Guo-Hui Cheng, Rui-Qing Ji, Yu Li

Abstract: Cortinarius is an important ectomycorrhizal genus that forms a symbiotic relationship with certain trees, shrubs and herbs. Recently, we began studying Cortinarius in China and here we describe three new species of Cortinarius subg. Telamonia based on morphological and ecological characteristics, together with phylogenetic analyses. Cortinarius laccariphyllus sp. nov. (section Colymbadini) is associated with broadleaf trees, with strongly hygrophanous basidiomata, special Laccaria-like lamellae and white and extremely sparse universal veil. Cortinarius neotorvus sp. nov. (section Telamonia) is associated with broadleaf trees and is easily confused with C. torvus, but can be distinguished by the colour of the fresh basidiomes and the stipe usually somewhat tapering towards the base. Cortinarius subfuscoperonatus sp. nov. (section Fuscoperonati) is associated with coniferous trees, with subglobose to broadly ellipsoid spores and is closely related to C. fuscoperonatus. A key to the new species and similar species in sections Colymbadini, Telamonia and Fuscoperonati is provided.

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Research Article Tue, 14 Jul 2020 16:37:26 +0300
Morphological and molecular identification of four new resupinate species of Lyomyces (Hymenochaetales) from southern China https://mycokeys.pensoft.net/article/48660/ MycoKeys 65: 101-118

DOI: 10.3897/mycokeys.65.48660

Authors: Jun-Zhu Chen, Chang-Lin Zhao

Abstract: Four new wood-inhabiting fungal species, Lyomyces bambusinus, L. cremeus, L. macrosporus and L. wuliangshanensis, are proposed based on a combination of morphological and molecular evidence. Lyomyces bambusinus is characterized by resupinate basidiomata with colliculose to tuberculate hymenial surface and broadly ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. Lyomyces cremeus is characterised by resupinate basidiomata with smooth, cream hymenial surface and ellipsoid, hyaline, thin-walled to slightly thick-walled basidiospores. Lyomyces macrosporus is characterized by pruinose basidiomata with reticulate hymenial surface, presence of three kinds of cystidia and larger basidiospores (6.7–8.9 × 4.4–5.4 µm). Lyomyces wuliangshanensis is characterized by coriaceous basidiomata and ellipsoid, hyaline, slightly thick-walled, smooth basidiospores. The phylogenetic analyses based on molecular data of the internal transcribed spacer (ITS) region sequences revealed that the four new species belonged to Lyomyces. Lyomyces bambusinus grouped with L. sambuci. Lyomyces cremeus clade was sister to a clade comprised of L. microfasciculatus. Lyomyces macrosporus was sister to L. allantosporus. Lyomyces wuliangshanensis was closely related to L. mascarensis.

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Research Article Thu, 26 Mar 2020 14:24:46 +0200
Diversity and community of culturable endophytic fungi from stems and roots of desert halophytes in northwest China https://mycokeys.pensoft.net/article/38923/ MycoKeys 62: 75-95

DOI: 10.3897/mycokeys.62.38923

Authors: Jia-Long Li, Xiang Sun, Yong Zheng, Peng-Peng Lü, Yong-Long Wang, Liang-Dong Guo

Abstract: Halophytes have high species diversity and play important roles in ecosystems. However, endophytic fungi of halophytes in desert ecosystems have been less investigated. In this study, we examined endophytic fungi associated with the stem and root of ten halophytic species colonizing the Gurbantonggut desert. A total of 36 endophytic fungal taxa were obtained, dominated by Alternaria eichhorniae, Monosporascus ibericus, and Pezizomycotina sp.1. The colonization rate and species richness of endophytic fungi varied in the ten plant species, with higher rates in roots than in stems. The endophytic fungal community composition was significantly affected by plant identity and tissue type. Some endophytic fungi showed significant host and tissue preferences. This finding suggests that host identity and tissue type structure endophytic fungal community in a desert ecosystem.

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Research Article Mon, 3 Feb 2020 14:14:09 +0200
A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species https://mycokeys.pensoft.net/article/38186/ MycoKeys 60: 45-67

DOI: 10.3897/mycokeys.60.38186

Authors: Xin-Cun Wang, Tie-Zhi Liu, Shuang-Lin Chen, Yi Li, Wen-Ying Zhuang

Abstract: Helvella species are ascomycetous macrofungi with saddle-shaped or cupulate apothecia. They are distributed worldwide and play an important ecological role as ectomycorrhizal symbionts. A recent multi-locus phylogenetic study of the genus suggested that the cupulate group of Helvella was in need of comprehensive revision. In this study, all the specimens of cupulate Helvella sensu lato with ribbed stipes deposited in HMAS were examined morphologically and molecularly. A four-locus phylogeny was reconstructed using partial sequences of the heat shock protein 90, nuclear rDNA internal transcribed spacer region 2, nuclear large subunit ribosomal DNA and translation elongation factor 1-α genes. Three clades were revealed in Helvella sensu stricto. Twenty species were included in the analysis, of which 13 are distributed in China. Three new species, H. acetabuloides, H. sichuanensis and H. tianshanensis, are described and illustrated in detail. A neotype was designated for H. taiyuanensis. Helvella calycina is a new record for China, while Dissingia leucomelaena should be excluded from Chinese mycota. Hsp90 and ITS2 are recommended as useful supplementary barcodes for species identifications of the genus.

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Research Article Thu, 31 Oct 2019 11:42:46 +0200
Three new species and a new combination of Triblidium https://mycokeys.pensoft.net/article/46645/ MycoKeys 60: 1-15

DOI: 10.3897/mycokeys.60.46645

Authors: Tu Lv, Cheng-Lin Hou, Peter R. Johnston

Abstract: Triblidiaceae (Rhytismatales) currently consists of two genera: Triblidium and Huangshania. Triblidium is the type genus and is characterised by melanized apothecia that occur scattered or in small clusters on the substratum, cleistohymenial (opening in the mesohymenial phase), inamyloid thin-walled asci and hyaline muriform ascospores. Before this study, only the type species, Triblidium caliciiforme, had DNA sequences in the NCBI GenBank. In this study, six specimens of Triblidium were collected from China and France and new ITS, mtSSU, LSU and RPB2 sequences were generated. Our molecular phylogenetic analysis and morphological study demonstrated three new species of Triblidium, which are formally described here: T. hubeiense, T. rostriforme and T. yunnanense. Additionally, our results indicated that Huangshania that was considered to be distinct from Triblidium because of its elongated, transversely-septate ascospores, is congeneric with Triblidium. Therefore, we have placed Huangshania in synonymy under Triblidium, rendering Triblidiaceae a monotypic family.

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Research Article Thu, 31 Oct 2019 08:55:42 +0200
Two new agaricoid species of the family Clavariaceae (Agaricales, Basidiomycota) from China, representing two newly recorded genera to the country https://mycokeys.pensoft.net/article/36416/ MycoKeys 57: 85-100

DOI: 10.3897/mycokeys.57.36416

Authors: Ming Zhang, Chao-Qun Wang, Tai-Hui Li

Abstract: Two new lamellar species, Camarophyllopsis olivaceogrisea and Hodophilus glaberripes, of the family Clavariaceae were discovered in the subtropical zone of China. Camarophyllopsis olivaceogrisea is morphologically characterized by its hygrophanous basidiomata, greenish gray to dull green pileus, shortly decurrent lamellae, broadly elliptic basidiospores 4–5.5 × 3.5–4.5 μm in size, and cutis-like pileipellis composed of cylindrical cells. Hodophilus glaberripes is mainly characterized by its white to brownish pileus, glabrous stipe, slight yam bean smell, broadly elliptic basidiospores 5–6.5 × 4–5 μm in size, and epithelium-like pileipellis composed of inflated cells. Phylogenetic placement of the two species was determined by the combined analyses of a DNA data matrix containing ITS and LSU, and showed that collections of the two species formed two independent lineages in the Camarophyllopsis and Hodophilus clades respectively. The delimitation of C. olivaceogrisea and H. glaberripes were evaluated using molecular, morphological, and ecological methods. This is the first report of the genera Camarophyllopsis and Hodophilus in China.

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Research Article Wed, 21 Aug 2019 16:43:10 +0300
Three new species of Dicephalospora from China as revealed by morphological and molecular evidences https://mycokeys.pensoft.net/article/33859/ MycoKeys 55: 87-99

DOI: 10.3897/mycokeys.55.33859

Authors: Huan-Di Zheng, Wen-Ying Zhuang

Abstract: Three new species of Dicephalospora are introduced based on morphological characters and DNA sequence analyses (maximum parsimony and neighbor-joining methods), viz. D. albolutea, D. shennongjiana, and D. yunnanica. All of them lack mucilaginous caps at ascospore poles. Dicephalospora albolutea is distinguished by cream to yellowish white apothecia and slightly curved ascospores. Dicephalospora shennongjiana is characterized by yellow apothecia, elliptical-fusoid ascospores 19−22 × 7−8.8 μm, and J+ asci 130−150 × 14−16.5 μm. Dicephalospora yunnanica is distinguished by orange apothecia and fusoid ascospores 16.5−25.3 × 3.3−3.5 μm. Descriptions and illustrations of the new species as well as a key to the known species in the genus are provided.

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Research Article Mon, 1 Jul 2019 09:22:45 +0300
Erysiphe deutziicola sp. nov. (Erysiphaceae, Ascomycota), a powdery mildew species found on Deutzia parviflora (Hydrangeaceae) with unusual appendages https://mycokeys.pensoft.net/article/34956/ MycoKeys 51: 97-106

DOI: 10.3897/mycokeys.51.34956

Authors: Peng-Lei Qiu, Uwe Braun, Yu Li, Shu-Yan Liu

Abstract: A powdery mildew (Erysiphales) has recently been collected on leaves of an ornamental shrub Deutzia parviflora in Baihua Mountain, Beijing, China. Microscopic examination of the chasmothecia suggested a species belonging to Erysiphe sect. Erysiphe, above all due to mycelioid chasmothecial appendages, although circinate apices of the appendages were rather in favour of Erysiphe sect. Uncinula, which is a fairly rare combination of appendage characteristics in Erysiphe. Phylogenetic analyses of ITS and 28S rDNA sequences demonstrated that the two examined powdery mildew collections on D. parviflora clustered together as an independent lineage within Erysiphe with 100% bootstrap support, representing a species of its own, which is phylogenetically allied to, but clearly distinct from Erysiphe deutziae and, in addition, morphologically quite different from all known Erysiphe species on hosts belonging to the Hydrangeaceae. The new species on D. parviflora is described as Erysiphe deutziicola.

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Research Article Wed, 8 May 2019 11:32:03 +0300
Four new species of Tremella (Tremellales, Basidiomycota) based on morphology and DNA sequence data https://mycokeys.pensoft.net/article/29180/ MycoKeys 47: 75-95

DOI: 10.3897/mycokeys.47.29180

Authors: Ying Zhao, Xin-zhan Liu, Feng-yan Bai

Abstract: In the present study, a total of 33 Tremella specimens in China were collected and examined using molecular phylogenetic analysis based on a combined sequence dataset of the nuc rDNA internal transcribed spacer (ITS) region and nuc 28S rDNA D1/D2 domain in conjunction with the morphological characters. Four new species, namely Tremella basidiomaticola, T. cheejenii, T. erythrina, and T. salmonea, are newly described based on their distinct phylogenetic relationships and the comparison of morphological characters with known Tremella species. Our results indicate a high species diversity of Tremella waiting to be discovered.

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Research Article Wed, 27 Feb 2019 10:44:31 +0200
Neostagonosporella sichuanensis gen. et sp. nov. (Phaeosphaeriaceae, Pleosporales) on Phyllostachys heteroclada (Poaceae) from Sichuan Province, China https://mycokeys.pensoft.net/article/32458/ MycoKeys 46: 119-150

DOI: 10.3897/mycokeys.46.32458

Authors: Chun-Lin Yang, Xiu-Lan Xu, Dhanushka N. Wanasinghe, Rajesh Jeewon, Rungtiwa Phookamsak, Ying-Gao Liu, Li-Juan Liu, Kevin D. Hyde

Abstract: Neostagonosporella sichuanensis sp. nov. was found on Phyllostachys heteroclada collected from Sichuan Province in China and is introduced in a new genus Neostagonosporella gen. nov. in this paper. Evidence for the placement of the new taxon in the family Phaeosphaeriaceae is supported by morphology and phylogenetic analysis of a combined LSU, SSU, ITS and TEF 1-α DNA sequence dataset. Maximum-likelihood, maximum-parsimony and Bayesian inference phylogenetic analyses support Neostagonosporella as a distinct genus within this family. The new genus is compared with related genera of Phaeosphaeriaceae and full descriptions and illustrations are provided. Neostagonosporella is characterised by its unique suite of characters, such as multiloculate ascostromata and cylindrical to fusiform, transversely multiseptate, straight or curved ascospores, which are widest at the central cells. Conidiostromata are multiloculate, fusiform to long fusiform or rhomboid, with two types conidia; macroconidia vermiform or subcylindrical to cylindrical, transversely multiseptate, sometimes curved, almost equidistant between septa and microconidia oval, ellipsoidal or long ellipsoidal, aseptate, rounded at both ends. An updated phylogeny of the Phaeosphaeriaceae based on multigene analysis is provided.

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Research Article Mon, 18 Feb 2019 09:18:44 +0200
Lecanicillium cauligalbarum sp. nov. (Cordycipitaceae, Hypocreales), a novel fungus isolated from a stemborer in the Yao Ren National Forest Mountain Park, Guizhou https://mycokeys.pensoft.net/article/30203/ MycoKeys 43: 59-74

DOI: 10.3897/mycokeys.43.30203

Authors: Ye-Ming Zhou, Jun-Rui Zhi, Mao Ye, Zhi-Yuan Zhang, Wen-Bo Yue, Xiao Zou

Abstract: A new species of entomopathogenic fungi, Lecanicillium cauligalbarum, was discovered from a survey of invertebrate-associated fungi in the Yao Ren National Forest Mountain Park in China. The synnemata of this species emerged from the corpse of a stemborer (Lepidoptera), which was hidden amongst pieces of wood on the forest floor. It differs from morphologically similar Lecanicillium species mainly in its short conidiogenous cells and ellipsoid to ovoid and aseptate conidia. Phylogenetic analysis of a combined data set comprising ITS, SSU, LSU, TEF, RPB1 and RPB2 sequence data supported the inclusion of L. cauligalbarum in the Lecanicillium genus and its recognition as a distinct species.

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Research Article Tue, 4 Dec 2018 13:55:06 +0200
Two new species of Geejayessia (Hypocreales) from Asia as evidenced by morphology and multi-gene analyses https://mycokeys.pensoft.net/article/27664/ MycoKeys 42: 7-19

DOI: 10.3897/mycokeys.42.27664

Authors: Zhao-Qing Zeng, Wen-Ying Zhuang

Abstract: Two new species of Geejayessia are introduced, based on materials collected from central China. Geejayessia clavata sp. nov. is characterised by gregarious, red brownish to dark red, oval-subglobose to globose perithecia that are formed on a basal stroma; (4–7-)8-spored cylindrical asci; ellipsoidal or rarely broadly ellipsoidal, uniseptate, smooth or finely verruculose ascospores; clavate, aseptate microconidia and absence of macroconidia. Geejayessia sinica sp. nov. is characterised by red to bright red, pyriform, subglobose to globose, perithecia on a basal stroma, collapsing laterally when dry; subcylindrical to clavate asci with a rounded apex; ellipsoidal, uniseptate ascospores; and falcate, multiseptate macroconidia with an arcuate tip. Morphological distinctions of the new species from the related fungi are discussed. This is the first report of Geejayessia from Asia.

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Research Article Tue, 6 Nov 2018 11:59:27 +0200
The genus Coprinellus (Basidiomycota; Agaricales) in Pakistan with the description of four new species https://mycokeys.pensoft.net/article/26743/ MycoKeys 39: 41-61

DOI: 10.3897/mycokeys.39.26743

Authors: Shah Hussain, Muhammad Usman, Najam-ul-Sehar Afshan, Habib Ahmad, Junaid Khan, Abdul Nasir Khalid

Abstract: Mushrooms with a thin-fleshed pileus that becomes plicate on opening, deliquescent lamellae and dark brown to blackish basidiospores are commonly called coprinoid mushrooms. The genus Coprinellus is one of the important lineages of coprinoid mushroom in the family Psathyrellaceae. Species-level taxonomy in Coprinellus is based mainly on the presence or absence and the structure of veil and cystidia on the pileus, of cystidia on the lamellae and on basidiospore morphology. In this study, four new species of Coprinellus (Co. campanulatus, Co. disseminatus-similis, Co. pakistanicus and Co. tenuis) are described from Pakistan. Species descriptions are based on morphological and molecular data. Phylogenetic analyses based on nuc rDNA ITS region show that the new species Co. campanulatus and Co. disseminatus-similis are clustered in a clade including members of section Micacei; Co. tenuis falls in a clade with members of section Domestici; and Co. pakistanicus recovered in a separate clade adjacent to other recently described clades of genus Coprinellus. Morpho-anatomical descriptions of the new species and comparison with closely allied taxa are provided. With this study, the number of known species of Coprinellus in Pakistan has reached eight.

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Research Article Tue, 11 Sep 2018 09:41:45 +0300
The Northeast Chinese species of Psathyrella (Agaricales, Psathyrellaceae) https://mycokeys.pensoft.net/article/24704/ MycoKeys 33: 85-102

DOI: 10.3897/mycokeys.33.24704

Authors: Jun-Qing Yan, Tolgor Bau

Abstract: Twenty seven species of Psathyrella have been found in Northeast China. Amongst them, P. conica, P. jilinensis, P. mycenoides and P. subsingeri are described as new species, based on studying morphological characteristics and phylogenetic analyses. Detailed morphological descriptions, line drawings and photographs of the new species are presented. Phylogenetic analysis of the nuclear ribosomal internal transcribed spacer (ITS) region and an identification key to the 27 Psathyrella species occuring in Northeast China are provided.

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Research Article Fri, 13 Apr 2018 10:00:58 +0300
The genus Parasola in Pakistan with the description of two new species https://mycokeys.pensoft.net/article/21430/ MycoKeys 30: 41-60

DOI: 10.3897/mycokeys.30.21430

Authors: Shah Hussain, Habib Ahmad, Sadiq Ullah, Najam-Ul-Sehar Afshan, Donald H. Pfister, Hassan Sher, Haidar Ali, Abdul N. Khalid

Abstract: Parasola is a genus of small, veil-less coprinoid mushrooms in the family Psathyrellaceae (Agaricales). The genus is not well documented in Asia, specifically in Pakistan. In this study we describe two new species Parasola glabra and P. pseudolactea from Pakistan, based on morphological and molecular data. Phylogeny based on three DNA regions: nuc rDNA region encompassing the internal transcribed spacers 1 and 2 along with the 5.8S rDNA (ITS), nuc 28S rDNA D1-D2 domains (28S) and translation elongation factor 1α gene (TEF1α) show that the new taxa are clustered in a clade formed by the members of section Parasola of genus Parasola. Parasola glabra with grayish pileus, slightly depressed pileal disc, lamellae separated from the stipe by pseudocollarium, basidiospores 14.5–16.5 × 9.5–11.5 × 8.0–10.5 µm, in front view broadly ovoid to oblong, some with rhomboidal outline, in side view ellipsoid, with eccentric germ-pore of 1.5 µm diameter. Parasola pseudolactea with yellowish brown to dull brown pileus, disc indistinctly umbonate, lamellae free, pseudocollarium absent, basidiospores 13.5–14.5 × 10.5–12.0 × 9.5–10.5 µm, in face view rounded triangular to heart shaped, rarely ovoid to subglobose, in side view ellipsoid to oblong, with eccentric germ-pore of 1.5 µm diam. In addition to these new species, P. auricoma and P. lilatincta were also studied. Morphological descriptions for the new species and comparison with known Parasola species are provided. Our observations highlight the diversity of Parasola in northern Pakistan and further document the need for additional systematic focus on the region’s fungi.

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Research Article Tue, 27 Feb 2018 09:59:56 +0200
Elaphroporia ailaoshanensis gen. et sp. nov. in Polyporales (Basidiomycota) https://mycokeys.pensoft.net/article/22086/ MycoKeys 29: 81-95

DOI: 10.3897/mycokeys.29.22086

Authors: Zi-Qiang Wu, Tai-Min Xu, Shan Shen, Xiang-Fu Liu, Kai-Yue Luo, Chang-Lin Zhao

Abstract: A new poroid wood-inhabiting fungal genus, Elaphroporia, typified by E. ailaoshanensis sp. nov., is proposed based on a combination of morphological features and molecular evidence. The genus is characterised by an annual growth habit, resupinate basidiocarps, becoming rigid and light-weight up on drying, a monomitic hyphal system with thick-walled generative hyphae bearing both clamp connections and simple septa, slightly amyloid, CB+ and ellipsoid, hyaline, thin-walled, smooth and IKI–, CB– basidiospores. Sequences of ITS and LSU nrRNA gene regions of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analysis based on molecular data of ITS+nLSU sequences showed that Elaphroporia belonged to the residual polyporoid clade and was closely related to Junghuhnia crustacea. Further investigation was obtained for more representative taxa in the Meruliaceae based on ITS+nLSU sequences, in which the result demonstrated that the genus Elaphroporia formed a monophyletic lineage with a strong support (100 % BS, 100 % BP, 1.00 BPP) and then grouped with Flaviporus and Steccherinum.

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Research Article Tue, 30 Jan 2018 10:31:41 +0200
Two new species of Xanthagaricus and some notes on Heinemannomyces from Asia https://mycokeys.pensoft.net/article/21029/ MycoKeys 28: 1-18

DOI: 10.3897/mycokeys.28.21029

Authors: Md. Iqbal Hosen, Zong-ping Song, Genevieve Gates, Samantha C. Karunarathna, M. Salahuddin M. Chowdhury, Tai-Hui Li

Abstract: Xanthagaricus flavosquamosus and X. necopinatus, two new species of Agaricaceae, are described and illustrated from Asia. Macroscopically, both species are closely related to each other, but there are obvious micromorphological and molecular differences between them. Morphological and phylogenetic data showed that the two new species are distinct from other known species of the genus Xanthagaricus. Xanthagaricus flavosquamosus from China is characterized by its small, yellow basidiomata, short clavate cheilocystidia, epithelial pileipellis, and verrucose basidiospores measuring 5–5.5 × 3–3.5 μm. Xanthagaricus necopinatus from Bangladesh is characterized by having small, yellow basidiomata, a fugacious annulus, clavate to narrowly clavate cheilocystidia, epithelial pileipellis, and rugulose-rough basidiospores measuring 4–5 × 2.7–3.2 μm. In addition to the new species, a Heinemannomyces collection from China is reported. Morphological data and molecular phylogenetic analyses fully support the Chinese collection being Heinemannomyces splendidissimus, a species of Agaricaceae, originally described from Southeast Asia. Detailed descriptions, color photos and illustrations of the three species are presented. A key to the genus Xanthagaricus occurring in Bangladesh and China is provided.

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Research Article Tue, 5 Dec 2017 11:20:20 +0200
Chlorovibrissea korfii sp. nov. from northern hemisphere and Vibrissea flavovirens new to China https://mycokeys.pensoft.net/article/14506/ MycoKeys 26: 1-11

DOI: 10.3897/mycokeys.26.14506

Authors: Huan-Di Zheng, Wen-Ying Zhuang

Abstract: A new species, Chlorovibrissea korfii, is described and illustrated, which is distinct from other members of the genus by the overall pale greenish apothecia 0.8–2.0 mm in diam. and 0.6–1.5 mm high, J+ asci 70–83 × 4.5–5.5 μm, and non-septate ascospores 44–52 × 1.2–1.5 μm. This is the first species of Chlorovibrissea reported from northern hemisphere. Vibrissea flavovirens is reported from China for the first time. Sequence analyses of the internal transcribed spacer of nuclear ribosomal DNA are used to confirm the affinity of the two taxa.

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Research Article Fri, 4 Aug 2017 09:24:48 +0300
Resolving the species of the lichen genus Graphina Müll. Arg. in China, with some new combinations https://mycokeys.pensoft.net/article/13154/ MycoKeys 25: 13-29

DOI: 10.3897/mycokeys.25.13154

Authors: Ze-feng Jia, Robert Lücking

Abstract: In the framework of continuing studies on the Graphidaceae in China, the status of all taxa traditionally assigned to the genus Graphina reported from China are resolved in the present paper. Five new combinations are made, namely Diorygma isabellinum (Zahlbr.) Z.F. Jia & Lücking, comb. nov., Fissurina adscribens (Nyl.) Z.F. Jia & Lücking, comb. nov., Graphis lecanactiformis (Zahlbr.) Z.F. Jia & Lücking, comb. nov., Phaeographis haloniata (Zahlbr.) Z.F. Jia & Lücking, comb. nov. and Platygramme taiwanensis (J.C. Wei) Z.F. Jia & Lucking, comb. nov. Five new synonymies were found: Graphina olivascens Zahlbr. (= Fissurina adscribens), Graphina plumbicolor Zahlbr. (= Phaeographis haloniata), Graphina roridula Zahlbr. and its variety platypoda Zahlbr. [= Diorygma pachygraphum (Nyl.) Kalb, Staiger & Elix], and Graphina taiwanensis f. obscurata J.C. Wei (= Platygramme taiwanensis).

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Checklist Mon, 10 Jul 2017 08:58:49 +0300
Resolving the genus Phaeographina Müll. Arg. in China https://mycokeys.pensoft.net/article/11986/ MycoKeys 21: 13-32

DOI: 10.3897/mycokeys.21.11986

Authors: Ze-Feng Jia, Robert Lücking

Abstract: As part of ongoing studies of the lichen family Graphidaceae in China, the status of all taxa traditionally assigned to the genus Phaeographina reported from China is resolved in the present paper. Five new combinations are proposed: Phaeographis pleiospora (Zahlbr.) Z.F. Jia & Lücking, comb. nov., Platygramme elaeoplaca (Zahlbr.) Z.F. Jia & Lücking, comb. nov., Platythecium maximum (Groenh.) Z.F. Jia & Lücking, comb. nov., P. pyrrhochroa (Mont. & Bosch) Z.F. Jia & Lücking, comb. nov., and Sarcographina heterospora (Nyl.) Z.F. Jia & Lücking, comb. nov. Six new synonyms are established: Phaeographina callospora Zahlbr. [= Diorygma hieroglyphicum (Pers.) Staiger & Kalb], P. fukiensis Zahlbr. [= Pallidogramme chrysenteron (Mont.) Staiger, Kalb & Lücking], P. fukiensis var. substriata Zahlbr. [= Pallidogramme chrysenteron (Mont.) Staiger, Kalb & Lücking], P. granulans Zahlbr. [= Platygramme platyloma (Müll. Arg.) M. Nakan. & Kashiw.], P. pluvisilvarum Zahlbr. [= Graphis alpestris (Zahlbr.) Staiger], and P. valida Zahlbr. [= Thecographa prosiliens (Mont. & Bosch) A. Massal.]. Two additional synonyms are reported: Phaeographina subrigida (Nyl.) Zahlbr. is synonymized under Platygramme platyloma (Müll. Arg.) M. Nakan. & Kashiw., and Platythecium dimorphodes (Nyl.) Staiger under Platythecium pyrrhochroum (Mont. & Bosch) Z.F. Jia & Lücking.

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Checklist Wed, 22 Mar 2017 12:25:20 +0200
Psora altotibetica (Psoraceae, Lecanorales), a new lichen species from the Tibetan part of the Himalayas https://mycokeys.pensoft.net/article/8824/ MycoKeys 13: 35-48

DOI: 10.3897/mycokeys.13.8824

Authors: Einar Timdal, Walter Obermayer, Mika Bendiksby

Abstract: In the present study, we describe the new species, Psora altotibetica, from nine localities in China (Tibetan area) and Nepal. The study includes analyses of anatomy, secondary chemistry, and DNA sequence data of P. altotibetica and presumed close relatives. Psora altotibetica resembles P. indigirkae morphologically, but is phylogenetically closer to P. tenuifolia and P. vallesiaca. It differs from P. indigirkae in the colour of the apothecia, the size of the ascospores, and in the secondary chemistry. The species is terricolous and was collected in the alpine zone of the Great Himalayas between 4230 and 5000 m altitude. Psora tenuifolia and P. vallesiaca are here reported as new to China and the Himalayas.

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Research Article Fri, 13 May 2016 09:57:35 +0300
DNA barcode identification of lichen-forming fungal species in the Rhizoplaca melanophthalma species-complex (Lecanorales, Lecanoraceae), including five new species https://mycokeys.pensoft.net/article/1201/ MycoKeys 7: 1-22

DOI: 10.3897/mycokeys.7.4508

Authors: Steven Leavitt, Fernando Fernández-Mendoza, Sergio Pérez-Ortega, Mohammad Sohrabi, Pradeep Divakar, Thorsten Lumbsch, Larry St. Clair

Abstract: Recent studies using sequence data from multiple loci and coalescent-based species delimitation have revealed several species-level lineages within the phenotypically circumscribed taxon Rhizoplaca melanophthalma sensu lato. Here, we formally describe five new species within this group, R. occulta, R. parilis, R. polymorpha, R. porterii, and R. shushanii, using support from the coalescent-based species delimitation method implemented in the program Bayesian Phylogenetics and Phylogeography (BPP) as the diagnostic feature distinguishing new species. We provide a reference DNA sequence database using the ITS marker as a DNA barcode for identifying species within this complex. We also assessed intraspecific genetic distances within the six R. melanophthalma sensu lato species. While intraspecific genetic distances within the five new species were less than or equal to the lowest interspecific pairwise comparison values, an overlap in genetic distances within the R. melanophthalma sensu stricto clade suggests the potential for additional phenotypically cryptic lineages within this broadly distributed lineage. Overall, our results demonstrate the potential for accurately identifying species within the R. melanophthalma group by using molecular-based identification methods.

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Research Article Thu, 9 May 2013 00:00:00 +0300