Latest Articles from MycoKeys Latest 88 Articles from MycoKeys https://mycokeys.pensoft.net/ Fri, 29 Mar 2024 12:10:34 +0200 Pensoft FeedCreator https://mycokeys.pensoft.net/i/logo.jpg Latest Articles from MycoKeys https://mycokeys.pensoft.net/ The Dolichens database: the lichen biota of the Dolomites https://mycokeys.pensoft.net/article/115462/ MycoKeys 103: 25-35

DOI: 10.3897/mycokeys.103.115462

Authors: Luana Francesconi, Matteo Conti, Gabriele Gheza, Stefano Martellos, Pier Luigi Nimis, Chiara Vallese, Juri Nascimbene

Abstract: The Dolichens project provides the first dynamic inventory of the lichens of the Dolomites (Eastern Alps, Italy). Occurrence records were retrieved from published and grey literature, reviewed herbaria, unpublished records collected by the authors, and new sampling campaigns, covering a period from 1820 to 2022. Currently, the dataset contains 56,251 records, referring to 1,719 infrageneric taxa, reported from 1820 to 2022, from hilly to nival belts, and corresponding to about half of the species known for the whole Alpine chain. Amongst them, 98% are georeferenced, although most of them were georeferenced a posteriori. The dataset is available through the Global Biodiversity Information Facility (GBIF; https://www.gbif.org/es/dataset/cea3ee2c-1ff1-4f8e-bb37-a99600cb4134) and through the Dolichens website (https://italic.units.it/dolichens/). We expect that this open floristic inventory will contribute to tracking the lichen diversity of the Dolomites over the past 200 years, and providing the basis for future taxonomic, biogeographical, and ecological studies.

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Data Paper Mon, 11 Mar 2024 17:55:40 +0200
Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora https://mycokeys.pensoft.net/article/116196/ MycoKeys 102: 155-181

DOI: 10.3897/mycokeys.102.116196

Authors: Łucja Ptach-Styn, Beata Guzow-Krzemińska, James C. Lendemer, Tor Tønsberg, Martin Kukwa

Abstract: Loxospora is a genus of crustose lichens containing 13 accepted species that can be separated into two groups, based on differences in secondary chemistry that correlate with differences in characters of the sexual reproductive structures (asci and ascospores). Molecular phylogenetic analyses recovered these groups as monophyletic and support their recognition as distinct genera that differ in phenotypic characters. Species containing 2’-O-methylperlatolic acid are transferred to the new genus, Chicitaea Guzow-Krzem., Kukwa & Lendemer and four new combinations are proposed: C. assateaguensis (Lendemer) Guzow-Krzem., Kukwa & Lendemer, C. confusa (Lendemer) Guzow-Krzem., Kukwa & Lendemer, C. cristinae (Guzow-Krzem., Łubek, Kubiak & Kukwa) Guzow-Krzem., Kukwa & Lendemer and C. lecanoriformis (Lumbsch, A.W. Archer & Elix) Guzow-Krzem., Kukwa & Lendemer. The remaining species produce thamnolic acid and represent Loxospora s.str. Haplotype analyses recovered sequences of L. elatina in two distinct groups, one corresponding to L. elatina s.str. and one to Pertusaria chloropolia, the latter being resurrected from synonymy of L. elatina and, thus, requiring the combination, L. chloropolia (Erichsen) Ptach-Styn, Guzow-Krzem., Tønsberg & Kukwa. Sequences of L. ochrophaea were found to be intermixed within the otherwise monophyletic L. elatina s.str. These two taxa, which differ in contrasting reproductive mode and overall geographic distributions, are maintained as distinct, pending further studies with additional molecular loci. Lectotypes are selected for Lecanora elatina, Pertusaria chloropolia and P. chloropolia f. cana. The latter is a synonym of Loxospora chloropolia. New primers for the amplification of mtSSU are also presented.

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Research Article Mon, 19 Feb 2024 16:32:21 +0200
New nephridiophagid genera (Fungi, Chytridiomycota) in a mallow beetle and an earwig https://mycokeys.pensoft.net/article/111298/ MycoKeys 100: 245-260

DOI: 10.3897/mycokeys.100.111298

Authors: Renate Radek, Christian Wurzbacher, Jürgen F. H. Strassert

Abstract: Nephridiophagids are unicellular fungi (Chytridiomycota) that infect the Malpighian tubules of insects. Most species have been found in cockroach hosts and belong to the genus Nephridiophaga. Three additional genera have been described from beetles and an earwig. Here, we characterise morphologically and molecular phylogenetically the nephridiophagids of the European earwig Forficula auricularia and the mallow beetle Podagrica malvae. Their morphology and life cycle stages resemble those of other nephridiophagids, but their rRNA gene sequences support the existence of two additional genera. Whereas the earwig nephridiophagid (Nephridiochytrium forficulae gen. nov. et sp. nov.) forms a sister lineage of the Nephridiophaga cluster, the mallow beetle nephridiophagid (Malpighivinco podagricae gen. nov. et sp. nov.) represents the earliest divergent lineage within the nephridiophagids, being sister to all other species. Our results corroborate the hypothesis that different insect groups harbour distinct nephridiophagid lineages.

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Research Article Fri, 22 Dec 2023 18:54:48 +0200
Exploring diversity within the genus Tulostoma (Basidiomycota, Agaricales) in the Pannonian sandy steppe: four fascinating novel species from Hungary https://mycokeys.pensoft.net/article/112458/ MycoKeys 100: 153-170

DOI: 10.3897/mycokeys.100.112458

Authors: Péter Finy, Mikael Jeppson, Dániel G. Knapp, Viktor Papp, László Albert, István Ölvedi, Károly Bóka, Dóra Varga, Gábor M. Kovács, Bálint Dima

Abstract: Steppe vegetation on sandy soil in Hungary has recently been revealed as one of the hot spots in Europe for the stalked puffballs (genus Tulostoma). In the framework of the taxonomic revision of gasteroid fungi in Hungary, four Tulostoma species are described here as new to science: T. dunense, T. hungaricum, T. sacchariolens and T. shaihuludii. The study is based on detailed macro- and micromorphological investigations (including light and scanning electron microscopy), as well as a three-locus phylogeny of nrDNA ITS, nrDNA LSU and tef1-α sequences. The ITS and LSU sequences generated from the type specimen of T. cretaceum are provided and this resolved partly the taxonomy of the difficult species complex of T. aff. cretaceum.

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Research Article Wed, 29 Nov 2023 18:17:34 +0200
A new genus Neobelonopsis and two new species of Trichobelonium (Helotiales, Ascomycota) discovered mainly from poaceous grasses native to Asia in Japan https://mycokeys.pensoft.net/article/90117/ MycoKeys 99: 45-85

DOI: 10.3897/mycokeys.99.90117

Authors: Hiyori Itagaki, Tsuyoshi Hosoya

Abstract: Mollisioid fungi, represented by Mollisia (Fr.) P. Karst., are characterized by soft, sessile apothecia with globose, dark-celled excipula, hyaline ascospores, and worldwide distribution in temperate regions. Their generic and species delimitation is difficult due to the lack of distinct features, and studies based on DNA sequences are urgently required. Two genera of mollisioid fungi, Belonopsis and Trichobelonium, comprise relatively few species and are recognized by (0–)1–3-septate ascospores, medullary excipulum composed of loosely interwoven hyphae, and calcium oxalate crystals in the excipulum. Specimens of undescribed species that are morphologically assignable to Belonopsis or Trichobelonium were collected from various sites in Japan and their assignment to the proper genera was attempted. According to a molecular phylogenetic analysis involving members of Mollisiaceae based on concatenated sequences of ITS, LSU, and RPB1, eight taxonomic entities were placed in a strongly supported single clade with Mollisia diesbachiana, separated from the type species of Belonopsis, B. excelsior. A new genus Neobelonopsis was thus proposed to accommodate the undescribed species. In this study, eight new species of Neobelonopsis and two new species of Trichobelonium were described. A new combination was also proposed for M. diesbachiana. The generic distinction of Neobelonopsis and Trichobelonium was supported by molecular analysis. Some additional characteristics to delimit Trichobelonium were identified, such as the presence of anchoring hyphae between the base of the apothecium and subiculum, and the production of abundant crystals and soluble pigments on the colonies. Derivative species of Neobelonopsis were found to have multi-septa in ascospores.

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Research Article Mon, 14 Aug 2023 17:49:26 +0300
Soil-borne Ophiostomatales species (Sordariomycetes, Ascomycota) in beech, oak, pine, and spruce stands in Poland with descriptions of Sporothrix roztoczensis sp. nov., S. silvicola sp. nov., and S. tumida sp. nov. https://mycokeys.pensoft.net/article/97416/ MycoKeys 97: 41-69

DOI: 10.3897/mycokeys.97.97416

Authors: Piotr Bilański, Robert Jankowiak, Halvor Solheim, Paweł Fortuna, Łukasz Chyrzyński, Paulina Warzecha, Stephen Joshua Taerum

Abstract: Ophiostomatales (Ascomycota) contains many species, most of which are associated with bark beetles. Some members of this order are plant or animal pathogens, while others colonize soil, different plant tissues, or even carpophores of some Basidiomycota. However, little is known about soil-inhabiting Ophiostomatales fungi. A survey of these fungi associated with soil under beech, oak, pine, and spruce stands in Poland yielded 623 isolates, representing 10 species: Heinzbutinia grandicarpa, Leptographium procerum, L. radiaticola, Ophiostoma piliferum, O. quercus, Sporothrix brunneoviolacea, S. dentifunda, S. eucastaneae, and two newly described taxa, namely Sporothrix roztoczensis sp. nov. and S. silvicola sp. nov. In addition, isolates collected from fallen shoots of Pinus sylvestris that were pruned by Tomicus sp. are described as Sporothrix tumida sp. nov. The new taxa were morphologically characterized and phylogenetically analyzed based on multi-loci sequence data (ITS, β-tubulin, calmodulin, and translation elongation factor 1-α genes). The Ophiostomatales species were especially abundant in soil under pine and oak stands. Leptographium procerum, S. silvicola, and S. roztoczensis were the most frequently isolated species from soil under pine stands, while S. brunneoviolacea was the most abundant in soil under oak stands. The results highlight that forest soil in Poland has a wide diversity of Ophiostomatales taxa, but further studies are required to uncover the molecular diversity and phylogenetic relationships of these fungi, as well as their roles in soil fungal communities.

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Research Article Tue, 16 May 2023 17:50:23 +0300
Mycobiont-specific primers facilitate the amplification of mitochondrial small subunit ribosomal DNA: a focus on the lichenized fungal genus Melanelia (Ascomycota, Parmeliaceae) in Iceland https://mycokeys.pensoft.net/article/100037/ MycoKeys 96: 57-75

DOI: 10.3897/mycokeys.96.100037

Authors: Maonian Xu, Yingkui Liu, Erik Möller, Scott LaGreca, Patricia Moya, Xinyu Wang, Einar Timdal, Hugo de Boer, Eva Barreno, Lisong Wang, Holger Thüs, Ólafur Andrésson, Kristinn Pétur Magnússon, Elín Soffia Ólafsdóttir, Starri Heiðmarsson

Abstract: The fungal mitochondrial small subunit (mtSSU) ribosomal DNA is one of the most commonly used loci for phylogenetic analysis of lichen-forming fungi, but their primer specificity to mycobionts has not been evaluated. The current study aimed to design mycobiont-specific mtSSU primers and highlights their utility with an example from the saxicolous lichen-forming fungal genus Melanelia Essl. in Iceland. The study found a 12.5% success rate (3 out of 24 specimens with good-quality mycobiont mtSSU sequences) using universal primers (i.e. mrSSU1 and mrSSU3R), not including off-target amplification of environmental fungi, e.g. Cladophialophora carrionii and Lichenothelia convexa. New mycobiont-specific primers (mt-SSU-581-5’ and mt-SSU-1345-3’) were designed by targeting mycobiont-specific nucleotide sites in comparison with environmental fungal sequences, and assessed for mycobiont primer specificity using in silico PCR. The new mycobiont-specific mtSSU primers had a success rate of 91.7% (22 out of 24 specimens with good-quality mycobiont mtSSU sequences) on the studied Melanelia specimens. Additional testing confirmed the specificity and yielded amplicons from 79 specimens of other Parmeliaceae mycobiont lineages. This study highlights the effectiveness of designing mycobiont-specific primers for studies on lichen identification, barcoding and phylogenetics.

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Research Article Tue, 21 Mar 2023 19:12:22 +0200
Taxonomy of Thelidium auruntii and T. incavatum complexes (lichenized Ascomycota, Verrucariales) in Finland https://mycokeys.pensoft.net/article/98738/ MycoKeys 96: 1-23

DOI: 10.3897/mycokeys.96.98738

Authors: Juha Pykälä, Annina Kantelinen, Leena Myllys

Abstract: The taxonomy of lichen species morphologically similar to Thelidium auruntii and T. incavatum in Finland is being revised. Based on ITS and morphology, ten species occur in Finland. All species are restricted to calcareous rocks. The Thelidium auruntii morphocomplex includes six species: T. auruntii, T. huuskonenii sp. nov., T. pseudoauruntii sp. nov., T. sallaense sp. nov, T. toskalharjiense sp. nov. and T. sp. 1. In the ITS phylogeny, T. auruntii, T. pseudoauruntii and T. sallaense group together, but the remaining species are placed outside of this clade. All the species have northern distribution in Finland, occurring on fells in NW Finland and/or in gorges in the Oulanka area in NE Finland. The Thelidium incavatum morphocomplex includes four species: T. declivum sp. nov., T. incavatum, T. mendax sp. nov. and T. sp. 2. This morphogroup is not resolved as monophyletic in the ITS phylogeny, with only T. declivum and T. mendax forming a strongly supported group. Thelidium incavatum is rather common in SW Finland, with one separate locality in eastern Finland. Thelidium declivum occurs only in the Oulanka area. Thelidium mendax occurs in the Oulanka area, but one locality is known from eastern central Finland. Thelidium sp. 2 is known from one locality in SW Lapland.

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Research Article Wed, 8 Mar 2023 21:14:50 +0200
A hotspot of lichen diversity and lichenological research in the Alps: the Paneveggio-Pale di San Martino Natural Park (Italy) https://mycokeys.pensoft.net/article/95858/ MycoKeys 94: 37-50

DOI: 10.3897/mycokeys.94.95858

Authors: Juri Nascimbene, Gabriele Gheza, Peter O. Bilovitz, Luana Francesconi, Josef Hafellner, Helmut Mayrhofer, Maurizio Salvadori, Chiara Vallese, Pier Luigi Nimis

Abstract: A checklist of 916 lichenised taxa is reported from the Paneveggio-Pale di San Martino Natural Park and its surroundings (Trentino-Alto Adige, N Italy), based on 7351 records from: (a) 72 literature sources, (b) eight public and private herbaria and (c) field observations by some of the authors. The study area appears as a hotspot of lichen diversity, hosting 30.1% of the lichen biota of the Alps in a territory that has 0.064% of their total surface area. This is mainly due to its high climatical, geological and orographic heterogeneity, but also to the long history of lichenological exploration, that started in the 19th century with Ferdinand Arnold and is still ongoing. The present work highlights the importance of detailed species inventories to support knowledge of biodiversity patterns, taxonomy and ecology and to properly address conservation issues. Fuscidea mollis var. caesioalbescens, Hydropunctaria scabra, Protoparmelia badia var. cinereobadia and Variospora paulii are new to Italy, 18 other taxa are new to Trentino-Alto Adige.

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Research Article Tue, 6 Dec 2022 15:00:01 +0200
New studies on Apiospora (Amphisphaeriales, Apiosporaceae): epitypification of Sphaeria apiospora, proposal of Ap. marianiae sp. nov. and description of the asexual morph of Ap. sichuanensis https://mycokeys.pensoft.net/article/87593/ MycoKeys 92: 63-78

DOI: 10.3897/mycokeys.92.87593

Authors: Ángel Pintos, Pablo Alvarado

Abstract: In the present work, an epitype for Sphaeria apiospora, the basionym of the type species of the genus Apiospora, Apiospora montagnei, is selected among collections growing in the host plant species reported in the original protologue, Arundo micrantha. Most samples obtained from localities near that of the lectotype (Perpignan, France) belong to the same species, which is not significantly different from the clade previously named Ap. phragmitis, suggesting that this name is a later synonym of Ap. montagnei. In addition, the name Ap. marianiae is here proposed to accommodate a newly discovered species found in the Balearic Islands (Spain), and the asexual state of Ap. sichuanensis is described for the first time from samples growing in the same islands.

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Research Article Tue, 23 Aug 2022 18:54:18 +0300
Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU) rRNA markers https://mycokeys.pensoft.net/article/77106/ MycoKeys 88: 1-33

DOI: 10.3897/mycokeys.88.77106

Authors: Angelina Ceballos-Escalera, John Richards, Maria Belen Arias, Daegan J. G. Inward, Alfried P. Vogler

Abstract: Full taxonomic characterisation of fungal communities is necessary for establishing ecological associations and early detection of pathogens and invasive species. Complex communities of fungi are regularly characterised by metabarcoding using the Internal Transcribed Spacer (ITS) and the Large-Subunit (LSU) gene of the rRNA locus, but reliance on a single short sequence fragment limits the confidence of identification. Here we link metabarcoding from the ITS2 and LSU D1-D2 regions to characterise fungal communities associated with bark beetles (Scolytinae), the likely vectors of several tree pathogens. Both markers revealed similar patterns of overall species richness and response to key variables (beetle species, forest type), but identification against the respective reference databases using various taxonomic classifiers revealed poor resolution towards lower taxonomic levels, especially the species level. Thus, Operational Taxonomic Units (OTUs) could not be linked via taxonomic classifiers across ITS and LSU fragments. However, using phylogenetic trees (focused on the epidemiologically important Sordariomycetes) we placed OTUs obtained with either marker relative to reference sequences of the entire rRNA cistron that includes both loci and demonstrated the largely similar phylogenetic distribution of ITS and LSU-derived OTUs. Sensitivity analysis of congruence in both markers suggested the biologically most defensible threshold values for OTU delimitation in Sordariomycetes to be 98% for ITS2 and 99% for LSU D1-D2. Studies of fungal communities using the canonical ITS barcode require corroboration across additional loci. Phylogenetic analysis of OTU sequences aligned to the full rRNA cistron shows higher success rate and greater accuracy of species identification compared to probabilistic taxonomic classifiers.

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Research Article Tue, 8 Mar 2022 10:28:13 +0200
New and interesting species of Penicillium (Eurotiomycetes, Aspergillaceae) in freshwater sediments from Spain https://mycokeys.pensoft.net/article/73861/ MycoKeys 86: 103-145

DOI: 10.3897/mycokeys.86.73861

Authors: Daniel Torres-Garcia, Josepa Gené, Dania García

Abstract: Penicillium species are common fungi found worldwide from diverse substrates, including soil, plant debris, food products and air. Their diversity in aquatic environments is still underexplored. With the aim to explore the fungal diversity in Spanish freshwater sediments, numerous Penicillium strains were isolated using various culture-dependent techniques. A preliminary sequence analysis of the β-tubulin (tub2) gene marker allowed us to identify several interesting species of Penicillium, which were later characterized phylogenetically with the barcodes recommended for species delimitation in the genus. Based on the multi-locus phylogeny of the internal transcribed spacer region (ITS) of the ribosomal DNA, and partial fragments of tub2, calmodulin (cmdA), and the RNA polymerase II largest subunit (rpb2) genes, in combination with phenotypic analyses, five novel species are described. These are P. ausonanum in section Lanata-Divaricata, P. guarroi in sect. Gracilenta, P. irregulare in sect. Canescentia, P. sicoris in sect. Paradoxa and P. submersum in sect. Robsamsonia. The study of several isolates from samples collected in different locations resulted in the reinstatement of P. vaccaeorum into section Citrina. Finally, P. heteromorphum (sect. Exilicaulis) and P. tardochrysogenum (sect. Chrysogena) are reported, previously only known from Antarctica and China, respectively.

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Research Article Tue, 1 Feb 2022 10:14:11 +0200
Two new species of Diaporthe (Diaporthaceae, Diaporthales) associated with tree cankers in the Netherlands https://mycokeys.pensoft.net/article/73107/ MycoKeys 85: 31-56

DOI: 10.3897/mycokeys.85.73107

Authors: Ning Jiang, Hermann Voglmayr, Chun-Gen Piao, Yong Li

Abstract: Diaporthe (Diaporthaceae, Diaporthales) is a common fungal genus inhabiting plant tissues as endophytes, pathogens and saprobes. Some species are reported from tree branches associated with canker diseases. In the present study, Diaporthe samples were collected from Alnus glutinosa, Fraxinus excelsior and Quercus robur in Utrecht, the Netherlands. They were identified to species based on a polyphasic approach including morphology, pure culture characters, and phylogenetic analyses of a combined matrix of partial ITS, cal, his3, tef1 and tub2 gene regions. As a result, four species (viz. Diaporthe pseudoalnea sp. nov. from Alnus glutinosa, Diaporthe silvicola sp. nov. from Fraxinus excelsior, D. foeniculacea and D. rudis from Quercus robur) were revealed from tree branches in the Netherlands. Diaporthe pseudoalnea differs from D. eres (syn. D. alnea) by its longer conidiophores. Diaporthe silvicola is distinguished from D. fraxinicola and D. fraxini-angustifoliae by larger alpha conidia.

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Research Article Mon, 29 Nov 2021 12:00:12 +0200
Lack of knowledge on ecological determinants and cryptic lifestyles hinder our understanding of Terfezia diversity https://mycokeys.pensoft.net/article/71372/ MycoKeys 84: 1-14

DOI: 10.3897/mycokeys.84.71372

Authors: Celeste Santos-Silva, Rogério Louro, Bruno Natário, Tânia Nobre

Abstract: Developing below the soil surface desert, truffles are hard to find. Within Terfezia genus, at least 18 species are described and many are endemic to the Mediterranean basin. Ecological and geographic information are key factors for species diagnosis, and so far Terfezia species are believed to be linked to either acidic or basic soils or to specific plant hosts. Thus, we have looked at Terfezia diversity within a relatively homogeneous geographical area in Portugal that is suitable for these species and that covered different soils and different dominant host species. We analyzed the observed intraspecific variability within the context of species ecological preferences (e. g. edaphic and putative host). One of our major findings was the discovery of T. grisea in acid soils in association with Tuberaria guttata, a puzzling information since, until now, this species was only found in alkaline soils. We also report on the linkage of different Terfezia lineages within species and ecologic parameters such as soil texture, soil pH and plant host. Additionally, by placing the collected specimens on the most recent genus phylogeny based on the ITS region, we also updated the number of known Terfezia species occurring in Portugal from three to ten. Terfezia dunensis is here reported for the first time for Portugal. Overall, our results show that the exploration of undersampled sites reveals itself as a good strategy to disclose unknown aspects of desert truffle diversity and ecology. These aspects are of prime importance when considering the economic value of the desert truffles for rural populations in the Mediterranean basin.

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Research Article Mon, 18 Oct 2021 11:44:56 +0300
Refining the picture: new records to the lichen biota of Italy https://mycokeys.pensoft.net/article/69027/ MycoKeys 82: 97-137

DOI: 10.3897/mycokeys.82.69027

Authors: Juri Nascimbene, Gabriele Gheza, Josef Hafellner, Helmut Mayrhofer, Lucia Muggia, Walter Obermayer, Göran Thor, Pier Luigi Nimis

Abstract: Based on the analysis of both historical and recent collections, this paper reports an annotated list of taxa which are new to the lichen biota of Italy or of its administrative regions. Specimens were identified using a dissecting and a compound microscope; routine chemical spot tests and standardized thin-layer chromatography (TLC or HPTLC). The list includes 225 records of 153 taxa. Twenty taxa are new to Italy, the others are new to one or more administrative regions, with 15 second records and 5 third records for Italy. Some of the species belong to recently-described taxa, others are poorly known, sterile or ephemeral lichens which were largely overlooked in Italy. Several species are actually rare, either because of the rarity of their habitats (e.g. old-growth forests), or because in Italy they are at the margins of their bioclimatic distribution. The picture of the lichen biota of Italy has now new pixels, but its grain is still coarse. Further analysis of historical collections, increased efforts in the exploration of some areas, and the taxonomic revision of critical groups are still necessary to provide more complete distributional data for new biogeographic hypotheses, taxonomic and ecological research, and biodiversity conservation.

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Research Article Wed, 11 Aug 2021 18:28:24 +0300
Six new species of Sporothrix from hardwood trees in Poland https://mycokeys.pensoft.net/article/66603/ MycoKeys 82: 1-32

DOI: 10.3897/mycokeys.82.66603

Authors: Agnieszka Ostafińska, Robert Jankowiak, Piotr Bilański, Halvor Solheim, Michael J. Wingfield

Abstract: Sporothrix (Sordariales, Ascomycota) is a well-supported monophyletic lineage within the Ophiostomatales, species of which occur in a diverse range of habitats including on forest trees, in the soil, associated with bark beetles and mites as well as on the fruiting bodies of some Basidiomycota. Several species have also been reported as important human and animal pathogens. During surveys of insect- and wound-associated Ophiostomatales from hardwood trees in Poland, many isolates with affinity to Sporothrix were recovered. In the present study, six undescribed Sporothrix spp. collected during these surveys are characterized based on their morphological characteristics and multi-locus phylogenenetic inference. They are described as Sporothrix cavum, Sporothrix cracoviensis, S. cryptarchum, S. fraxini, S. resoviensis, and S. undulata. Two of the Sporothrix spp. reside in the S. gossypina-complex, while one forms part of the S. stenoceras-complex. One Sporothrix sp. is a member of lineage F, and two other species grouped outside any of the currently defined species complexes. All the newly described species were recovered from hardwood habitats in association with sub-cortical insects, wounds or woodpecker cavities. These species were morphologically similar, with predominantly asexual states having hyaline or lightly pigmented conidia, which produce holoblastically on denticulate conidiogenous cells. Five of the new taxa produce ascomata with necks terminating in long ostiolar hyphae and allantoid ascospores without sheaths. The results suggest that Sporothrix species are common members of the Ophiostomatales in hardwood ecosystems of Poland.

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Research Article Wed, 4 Aug 2021 15:08:55 +0300
Corrigendum: Pykälä J, Kantelinen A, Myllys L (2020) Taxonomy of Verrucaria species characterised by large spores, perithecia leaving pits in the rock and a pale thin thallus in Finland. MycoKeys 72: 43–92. https://doi.org/10.3897/mycokeys.72.56223 https://mycokeys.pensoft.net/article/67870/ MycoKeys 80: 163-164

DOI: 10.3897/mycokeys.80.67870

Authors: Juha Pykälä, Annina Kantelinen, Leena Myllys

Abstract: Corrigendum

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Corrigenda Thu, 3 Jun 2021 12:01:33 +0300
The lichens of the Majella National Park (Central Italy): an annotated checklist https://mycokeys.pensoft.net/article/62362/ MycoKeys 78: 119-168

DOI: 10.3897/mycokeys.78.62362

Authors: Gabriele Gheza, Luca Di Nuzzo, Chiara Vallese, Renato Benesperi, Elisabetta Bianchi, Valter Di Cecco, Luciano Di Martino, Paolo Giordani, Josef Hafellner, Helmut Mayrhofer, Pier Luigi Nimis, Mauro Tretiach, Juri Nascimbene

Abstract: The botanical exploration of the Majella National Park has a long tradition dating back to the eighteenth century. However, the lichen biota of this area is still poorly investigated. To provide a baseline for future investigations, in this annotated checklist, we summarised all available information on the occurrence of lichens in the Majella National Park, retrieved from previous literature, herbarium material and original data produced by recent research. The checklist includes 342 infrageneric taxa. However, seven taxa are considered as dubious, thus setting the number of accepted taxa at 335, i.e. 45.8% of those currently known to occur in the Abruzzo Region. This checklist provides a baseline of the lichens known to occur in the Majella National Park, highlighting the potential of this area as a hotspot of lichen biodiversity, especially from a biogeographical point of view as indicated by the occurrence of several arctic-alpine species that form disjunct populations in the summit area of the massif.

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Checklist Mon, 29 Mar 2021 09:10:30 +0300
Novel species of Cladosporium from environmental sources in Spain https://mycokeys.pensoft.net/article/60862/ MycoKeys 77: 1-25

DOI: 10.3897/mycokeys.77.60862

Authors: Isabel Iturrieta-González, Dania García, Josepa Gené

Abstract: Cladosporium is a monophyletic genus in Cladosporiaceae (Cladosporiales, Dothideomycetes) whose species are mainly found as saprobes and endophytes, but it also includes fungi pathogenic for plants, animals and human. Species identification is currently based on three genetic markers, viz., the internal transcribed spacer regions (ITS) of the rDNA, and partial fragments of actin (act) and the translation elongation factor 1-α (tef1) genes. Using this phylogenetic approach and from morphological differences, we have recognized six new species originating from soil, herbivore dung and plant material collected at different Spanish locations. They are proposed as Cladosporium caprifimosum, C. coprophilum, C. fuscoviride and C. lentulum belonging in the C. cladosporioides species complex, and C. pseudotenellum and C. submersum belonging in the C. herbarum species complex. This study revealed that herbivore dung represented a reservoir of novel lineages in the genus Cladosporium.

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Research Article Tue, 5 Jan 2021 08:15:22 +0200
Corrigendum: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1–68. https://doi.org/10.3897/mycokeys.73.47287 https://mycokeys.pensoft.net/article/59175/ MycoKeys 74: 109-110

DOI: 10.3897/mycokeys.74.59175

Authors: Richard Spjut, Antoine Simon, Martin Guissard, Nicolas Magain, Emmanuël Sérusiaux

Abstract: None

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Corrigenda Fri, 30 Oct 2020 08:55:29 +0200
Delimitation, new species and teleomorph-anamorph relationships in Codinaea, Dendrophoma, Paragaeumannomyces and Striatosphaeria (Chaetosphaeriaceae) https://mycokeys.pensoft.net/article/57824/ MycoKeys 74: 17-74

DOI: 10.3897/mycokeys.74.57824

Authors: Martina Réblová, Jana Nekvindová, Jacques Fournier, Andrew N. Miller

Abstract: The Chaetosphaeriaceae are a diverse group of pigmented, predominantly phialidic hyphomycetes comprised of several holomorphic genera including Chaetosphaeria, the most prominent genus of the family. Although the morphology of the teleomorphs of the majority of Chaetosphaeria is rather uniform, their associated anamorphs primarily exhibit the variability and evolutionary change observed in the genus. An exception from the morphological monotony among Chaetosphaeria species is a group characterised by scolecosporous, hyaline to light pink, multiseptate, asymmetrical ascospores and a unique three-layered ascomatal wall. Paragaeumannomyces sphaerocellularis, the type species of the genus, exhibits these morphological traits and is compared with similar Chaetosphaeria with craspedodidymum- and chloridium-like synanamorphs. Morphological comparison and phylogenetic analyses of the combined ITS-28S sequences of 35 isolates and vouchers with these characteristics revealed a strongly-supported, morphologically well-delimited clade in the Chaetosphaeriaceae containing 16 species. The generic name Paragaeumannomyces is applied to this monophyletic clade; eight new combinations and five new species, i.e. P. abietinus sp. nov., P. elegans sp. nov., P. granulatus sp. nov., P. sabinianus sp. nov. and P. smokiensis sp. nov., are proposed. A key to Paragaeumannomyces is provided. Using morphology, cultivation studies and phylogenetic analyses of ITS and 28S rDNA, two additional new species from freshwater and terrestrial habitats, Codinaea paniculata sp. nov. and Striatosphaeria castanea sp. nov., are described in the family. A codinaea-like anamorph of S. castanea forms conidia with setulae at each end in axenic culture; this feature expands the known morphology of Striatosphaeria. A chaetosphaeria-like teleomorph is experimentally linked to Dendrophoma cytisporoides, a sporodochial hyphomycete and type species of Dendrophoma, for the first time.

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Research Article Mon, 19 Oct 2020 16:50:49 +0300
Caliciopsis moriondi, a new species for a fungus long confused with the pine pathogen C. pinea https://mycokeys.pensoft.net/article/53028/ MycoKeys 73: 87-108

DOI: 10.3897/mycokeys.73.53028

Authors: Duccio Migliorini, Nicola Luchi, Alessia Lucia Pepori, Francesco Pecori, Chiara Aglietti, Fabio Maccioni, Isabel Munck, Stephen Wyka, Kirk Broders, Michael J. Wingfield, Alberto Santini

Abstract: The genus Caliciopsis (Eurotiomycetes, Coryneliales) includes saprobic and plant pathogenic species. Caliciopsis canker is caused by Caliciopsis pinea Peck, a species first reported in the 19th century in North America. In recent years, increasing numbers of outbreaks of Caliciopsis canker have been reported on different Pinus spp. in the eastern USA. In Europe, the disease has only occasionally been reported causing cankers, mostly on Pinus radiata in stressed plantations. The aim of this study was to clarify the taxonomy of Caliciopsis specimens collected from infected Pinus spp. in Europe and North America using an integrative approach, combining morphology and phylogenetic analyses of three loci. The pathogenicity of the fungus was also considered. Two distinct groups were evident, based on morphology and multilocus phylogenetic analyses. These represent the known pathogen Caliciopsis pinea that occurs in North America and a morphologically similar, but phylogenetically distinct, species described here as Caliciopsis moriondi sp. nov., found in Europe and at least one location in eastern North America. Caliciopsis moriondi differs from C. pinea in various morphological features including the length of the ascomata, as well as their distribution on the stromata.

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Research Article Fri, 25 Sep 2020 08:31:16 +0300
The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history https://mycokeys.pensoft.net/article/47287/ MycoKeys 73: 1-68

DOI: 10.3897/mycokeys.73.47287

Authors: Richard Spjut, Antoine Simon, Martin Guissard, Nicolas Magain, Emmanuël Sérusiaux

Abstract: We present phylogenetic analyses of the fruticose Ramalinaceae based on extensive collections from many parts of the world, with a special focus on the Vizcaíno deserts in north-western Mexico and the coastal desert in Namibia. We generate a four-locus DNA sequence dataset for accessions of Ramalina and two additional loci for Niebla and Vermilacinia. Four genera are strongly supported: the subcosmopolitan Ramalina, the new genus Namibialina endemic to SW Africa, and a duo formed by Niebla and Vermilacinia, endemic to the New World except the sorediate V. zebrina that disjunctly occurs in Namibia. The latter three genera are restricted to coastal desert and chaparral where vegetation depends on moisture from ocean fog. Ramalina is subcosmopolitan and much more diverse in its ecology. We show that Ramalina and its sister genus Namibialina diverged from each other at c. 48 Myrs, whereas Vermilacinia and Niebla split at c. 30 Myrs. The phylogeny of the fruticose genera remains unresolved to their ancestral crustose genera. Species delimitation within Namibialina and Ramalina is rather straightforward. The phylogeny and taxonomy of Vermilacinia are fully resolved, except for the two youngest clades of corticolous taxa, and support current taxonomy, including four new taxa described here. Secondary metabolite variation in Niebla generally coincides with major clades which are comprised of species complexes with still unresolved phylogenetic relationships. A micro-endemism pattern of allopatric species is strongly suspected for both genera, except for the corticolous taxa within Vermilacinia. Both Niebla and saxicolous Vermilacinia have chemotypes unique to species clades that are largely endemic to the Vizcaíno deserts. The following new taxa are described: Namibialina gen. nov. with N. melanothrix (comb. nov.) as type species, a single new species of Ramalina (R. krogiae) and four new species of Vermilacinia (V. breviloba, V. lacunosa, V. pustulata and V. reticulata). The new combination V. granulans is introduced. Two epithets are re-introduced for European Ramalina species: R. crispans (= R. peruviana auct. eur.) and R. rosacea (= R. bourgeana auct. p.p). A lectotype is designated for Vermilacinia procera. A key to saxicolous species of Vermilacinia is presented.

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Research Article Fri, 11 Sep 2020 14:49:22 +0300
Taxonomy of Verrucaria species characterised by large spores, perithecia leaving pits in the rock and a pale thin thallus in Finland https://mycokeys.pensoft.net/article/56223/ MycoKeys 72: 43-92

DOI: 10.3897/mycokeys.72.56223

Authors: Juha Pykälä, Annina Kantelinen, Leena Myllys

Abstract: Species of Verrucaria, characterised by large spores (at least some spores exceeding 25 µm in length), perithecia leaving pits in the rock and a pale thin thallus, form a taxonomically-difficult and poorly-known group. In this study, such species occurring in Finland are revised, based on ITS sequences and morphology. Maximum likelihood analysis of ITS sequence data was used to examine if the species belong to the Thelidium group, as suggested by BLAST search. Twelve species are accepted in Finland: Verrucaria bifurcata sp. nov., V. cavernarum sp. nov., V. devergens, V. difficilis sp. nov., V. foveolata, V. fuscozonata sp. nov., V. karelica, V. kuusamoensis sp. nov., V. subdevergens sp. nov., V. subjunctiva, V. subtilis and V. vacillans sp. nov. Verrucaria foveolata is nested in V. subjunctiva in the phylogeny, but due to morphological and ecogeographical differences, the two taxa are treated as separate species pending further studies. Based on the analysis, the study species belong to the Thelidium group. The studied species show a rather high infraspecific morphological, but a low genetic variation. Furthermore, they show considerable overlap in their morphology and many specimens cannot be reliably identified, based on morphology only. All species are restricted to calcareous rocks. Verrucaria alpigena, V. cinereorufa and V. hochstetteri are excluded from the lichen flora of Finland. Verrucaria grossa is considered a species with unresolved identity. Verrucaria foveolata and V. subtilis are rather common on calcareous rocks of Finland while V. devergens and V. kuusamoensis are restricted to northern Finland. Verrucaria subjunctiva occurs mainly in northern Finland. Verrucaria bifurcata has been found only from southern Finland. Verrucaria difficilis has few localities both in SW and NE Finland. Verrucaria vacillans is restricted to calcareous rocks (dolomite) on the mountains of the NW corner of Finland. Verrucaria fuscozonata, V. karelica and V. subdevergens occur only in the Oulanka area in NE Finland. A lectotype is designated for V. subjunctiva. The morphology of the Finnish species was compared with 51 European species of Verrucaria presumably belonging to the Thelidium group.

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Research Article Wed, 2 Sep 2020 08:51:52 +0300
Checklist of thallus-forming Laboulbeniomycetes from Belgium and the Netherlands, including Hesperomyces halyziae and Laboulbenia quarantenae spp. nov. https://mycokeys.pensoft.net/article/53421/ MycoKeys 71: 23-86

DOI: 10.3897/mycokeys.71.53421

Authors: Danny Haelewaters, André De Kesel

Abstract: In this paper we present an updated checklist of thallus-forming Laboulbeniomycetes (Ascomycota, Pezizomycotina), that is, the orders Herpomycetales and Laboulbeniales, from Belgium and the Netherlands. Two species are newly described based on morphology, molecular data (ITS, LSU ribosomal DNA) and ecology (host association). These are Hesperomyces halyziae on Halyzia sedecimguttata (Coleoptera, Coccinellidae) from both countries and Laboulbenia quarantenae on Bembidion biguttatum (Coleoptera, Carabidae) from Belgium. In addition, nine new country records are presented. For Belgium: Laboulbenia aubryi on Amara aranea (Coleoptera, Carabidae) and Rhachomyces spinosus on Syntomus foveatus (Coleoptera, Carabidae). For the Netherlands: Chitonomyces melanurus on Laccophilus minutus (Coleoptera, Dytiscidae), Euphoriomyces agathidii on Agathidium laevigatum (Coleoptera, Leiodidae), Laboulbenia fasciculata on Omophron limbatum (Coleoptera, Carabidae), Laboulbenia metableti on Syntomus foveatus and S. truncatellus (Coleoptera, Carabidae), Laboulbenia pseudomasei on Pterostichus melanarius (Coleoptera, Carabidae), Rhachomyces canariensis on Trechus obtusus (Coleoptera, Carabidae), and Stigmatomyces hydrelliae on Hydrellia albilabris (Diptera, Ephydridae). Finally, an identification key to 140 species of thallus-forming Laboulbeniomycetes in Belgium and the Netherlands is provided. Based on the combined data, we are able to identify mutual gaps that need to be filled as well as weigh the impact of chosen strategies (fieldwork, museum collections) and techniques in these neighboring countries. The aim of this work is to serve as a reference for studying Laboulbeniomycetes fungi in Europe.

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Research Article Thu, 30 Jul 2020 09:01:52 +0300
Corrigendum: Bien S, Damm U (2020) Arboricolonus simplex gen. et sp. nov. and novelties in Cadophora, Minutiella and Proliferodiscus from Prunus wood in Germany. MycoKeys 63: 163–172. https://doi.org/10.3897/mycokeys.63.46836 https://mycokeys.pensoft.net/article/55264/ MycoKeys 69: 111-112

DOI: 10.3897/mycokeys.69.55264

Authors: Steffen Bien, Ulrike Damm

Abstract: In the original article the herbarium code of the Senckenberg Mu­seum of Natural History Görlitz, Germany, was wrongly cited as GLMC. The correct herbarium code of the institution is GLM.We combined Margarinomyces bubakii in the genus Cadophora. This was not compliant with the International Code of Nomen­clature for algae, fungi, and plants article F.5.1 (Turland et al. 2018), because a registration identifier was lacking, which is compulsory since 1 January 2013. The combination is now validated by providing the MycoBank number below.

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Corrigenda Tue, 14 Jul 2020 10:47:46 +0300
Novel species of Huntiella from naturally-occurring forest trees in Greece and South Africa https://mycokeys.pensoft.net/article/53205/ MycoKeys 69: 33-52

DOI: 10.3897/mycokeys.69.53205

Authors: FeiFei Liu, Seonju Marincowitz, ShuaiFei Chen, Michael Mbenoun, Panaghiotis Tsopelas, Nikoleta Soulioti, Michael J. Wingfield

Abstract: Huntiella species are wood-infecting, filamentous ascomycetes that occur in fresh wounds on a wide variety of tree species. These fungi are mainly known as saprobes although some have been associated with disease symptoms. Six fungal isolates with typical culture characteristics of Huntiella spp. were collected from wounds on native forest trees in Greece and South Africa. The aim of this study was to identify these isolates, using morphological characters and multigene phylogenies of the rRNA internal transcribed spacer (ITS) region, portions of the β-tubulin (BT1) and translation elongation factor 1α (TEF-1α) genes. The mating strategies of these fungi were also determined through PCR amplification of mating type genes. The study revealed two new species; one from Platanus orientalis in Greece and one from Colophospermum mopane and Senegalia nigrescens in South Africa. These novel taxa have been provided with the names, H. hellenica sp. nov. and H. krugeri sp. nov., respectively. The former species was found to have a homothallic and the latter a heterothallic mating system.

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Research Article Fri, 10 Jul 2020 08:39:02 +0300
Two new species of Ophiostomatales (Sordariomycetes) associated with the bark beetle Dryocoetes alni from Poland https://mycokeys.pensoft.net/article/50035/ MycoKeys 68: 23-48

DOI: 10.3897/mycokeys.68.50035

Authors: Beata Strzałka, Robert Jankowiak, Piotr Bilański, Nikita Patel, Georg Hausner, Riikka Linnakoski, Halvor Solheim

Abstract: Bark beetles belonging to the genus Dryocoetes (Coleoptera, Curculionidae, Scolytinae) are known vectors of fungi, such as the pathogenic species Grosmannia dryocoetidis involved in alpine fir (Abies lasiocarpa) mortality. Associations between hardwood-infesting Dryocoetes species and fungi in Europe have received very little research attention. Ectosymbiotic fungi residing in Ceratocystiopsis and Leptographium (Ophiostomatales, Sordariomycetes, Ascomycota) were commonly detected in previous surveys of the Dryocoetes alni-associated mycobiome in Poland. The aim of this study was to accurately identify these isolates and to provide descriptions of the new species. The identification was conducted based on morphology and DNA sequence data for six loci (ITS1-5.8S, ITS2-28S, ACT, CAL, TUB2, and TEF1-α). This revealed two new species, described here as Ceratocystiopsis synnemata sp. nov. and Leptographium alneum sp. nov. The host trees for the new species included Alnus incana and Populus tremula. Ceratocystiopsis synnemata can be distinguished from its closely related species, C. pallidobrunnea, based on conidia morphology and conidiophores that aggregate in loosely arranged synnemata. Leptographium alneum is closely related to Grosmannia crassivaginata and differs from this species in having a larger ascomatal neck, and the presence of larger club-shaped cells.

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Research Article Wed, 17 Jun 2020 15:55:59 +0300
First record of Harpellales, Orphellales (Kickxellomycotina) and Amoebidiales (Mesomycetozoea) from Bulgaria, including a new species of Glotzia https://mycokeys.pensoft.net/article/52055/ MycoKeys 67: 55-80

DOI: 10.3897/mycokeys.67.52055

Authors: Laia Guàrdia Valle, Desislava Stoianova

Abstract: This paper presents the results obtained from a short survey performed in Bulgaria, southeast Europe, where the trichomycetes (sensu lato), an ecological group of arthropod gut endosymbionts, were previously completely unknown. The present study initiates the comprehension of these cryptic organisms, members of the Kickxellomycotina (Harpellales, Orphellales) and the Mesomycetozoea (Amoebidiales), in this Balkan country. Eighteen new geographic records for Bulgaria are reported, including 10 species of Harpellales, three species of Orphellales and five species of Amoebidiales. Within the Harpellales, the species Glotzia balkanensis sp. nov. is described. This new species is most related to the rare species G. centroptili Gauthier ex Manier & Lichtw. and G. stenospora White & Lichtw., but is differentiated by spore and thallial characteristics. Photographs are provided and biogeographic implications of these records are discussed.

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Research Article Wed, 27 May 2020 09:09:38 +0300
Discovery of a new species of the Hypoxylon rubiginosum complex from Iran and antagonistic activities of Hypoxylon spp. against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in dual culture https://mycokeys.pensoft.net/article/50946/ MycoKeys 66: 105-133

DOI: 10.3897/mycokeys.66.50946

Authors: Mohammad Javad Pourmoghaddam, Christopher Lambert, Frank Surup, Seyed Akbar Khodaparast, Irmgard Krisai-Greilhuber, Hermann Voglmayr, Marc Stadler

Abstract: During a survey of xylarialean fungi in Northern Iran, several specimens that showed affinities to the Hypoxylon rubiginosum complex were collected and cultured. A comparison of their morphological characters, combined with a chemotaxonomic study based on high performance liquid chromatography, coupled with diode array detection and mass spectrometry (HPLC-DAD/MS) and a multi-locus phylogeny based on ITS, LSU, rbp2 and tub2 DNA sequences, revealed a new species here described as Hypoxylon guilanense. In addition, Hypoxylon rubiginosum sensu stricto was also encountered. Concurrently, an endophytic isolate of the latter species showed strong antagonistic activities against the Ash Dieback pathogen, Hymenoscyphus fraxineus, in a dual culture assay in our laboratory. Therefore, we decided to test the new Iranian fungi for antagonistic activities against the pathogen, along with several cultures of other Hypoxylon species that are related to H. rubiginosum. Our results suggest that the antagonistic effects of Hypoxylon spp. against Hym. fraxineus are widespread and that they are due to the production of antifungal phomopsidin derivatives in the presence of the pathogen.

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Research Article Fri, 24 Apr 2020 14:27:15 +0300
Ochraceocephala foeniculi gen. et sp. nov., a new pathogen causing crown rot of fennel in Italy https://mycokeys.pensoft.net/article/48389/ MycoKeys 66: 1-22

DOI: 10.3897/mycokeys.66.48389

Authors: Dalia Aiello, Alessandro Vitale, Giancarlo Polizzi, Hermann Voglmayr

Abstract: A new disease of fennel is described from Sicily (southern Italy). Surveys of the disease and sampling were conducted during spring 2017 and 2018 in Adrano and Bronte municipalities (Catania province) where this crop is widely cultivated. Isolations from the margin of symptomatic tissues resulted in fungal colonies with the same morphology. Pathogenicity tests with one isolate of the fungus on 6-month-old plants of fennel reproduced similar symptoms to those observed in nature. Inoculation experiments to assess the susceptibility of six different fennel cultivars to infection by the pathogen showed that the cultivars ‘Narciso’, ‘Apollo’, and ‘Pompeo’ were more susceptible than ‘Aurelio’, ‘Archimede’, and ‘Pegaso’. Phylogenetic analyses based on a matrix of the internal transcribed spacer (ITS), the large subunit (LSU), and the small subunit (SSU) rDNA regions revealed that the isolates represent a new genus and species within the Leptosphaeriaceae, which is here described as Ochraceocephala foeniculi gen. et sp. nov. This study improves the understanding of this new fennel disease, but further studies are needed for planning effective disease management strategies. According to the results of the phylogenetic analyses, Subplenodomus iridicola is transferred to the genus Alloleptosphaeria and Acicuseptoria rumicis to Paraleptosphaeria.

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Research Article Mon, 30 Mar 2020 08:36:43 +0300
Citizen science project reveals high diversity in Didymellaceae (Pleosporales, Dothideomycetes) https://mycokeys.pensoft.net/article/47704/ MycoKeys 65: 49-99

DOI: 10.3897/mycokeys.65.47704

Authors: Lingwei Hou, Margarita Hernández-Restrepo, Johannes Zacharias Groenewald, Lei Cai, Pedro W. Crous

Abstract: Fungal communities play a crucial role in maintaining the health of managed and natural soil environments, which directly or indirectly affect the properties of plants and other soil inhabitants. As part of a Citizen Science Project initiated by the Westerdijk Fungal Biodiversity Institute and the Utrecht University Museum, which aimed to describe novel fungal species from Dutch garden soil, the diversity of Didymellaceae, which is one of the largest families in the Dothideomycetes was investigated. A preliminary analysis of the ITS and LSU sequences from the obtained isolates allowed the identification of 148 strains belonging to the family. Based on a multi-locus phylogeny of a combined ITS, LSU, rpb2 and tub2 alignment, and morphological characteristics, 20 different species were identified in nine genera, namely Ascochyta, Calophoma, Didymella, Juxtiphoma, Nothophoma, Paraboeremia, Phomatodes, Stagonosporopsis, and Xenodidymella. Several isolates confirmed to be ubiquitous plant pathogens or endophytes were for the first time identified from soil, such as Ascochyta syringae, Calophoma clematidis-rectae, and Paraboeremia litseae. Furthermore, one new genus and 12 novel species were described from soil: Ascochyta benningiorum sp. nov., Didymella degraaffiae sp. nov., D. kooimaniorum sp. nov., Juxtiphoma kolkmaniorum sp. nov., Nothophoma brennandiae sp. nov., Paraboeremia rekkeri sp. nov., P. truiniorum sp. nov., Stagonosporopsis stuijvenbergii sp. nov., S. weymaniae sp. nov., Vandijckomycella joseae gen. nov. et sp. nov., V. snoekiae sp. nov., and Xenodidymella weymaniae sp. nov. From the results of this study, soil was revealed to be a rich substrate for members of Didymellaceae, several of which were previously known only from diseased or apparently healthy plant hosts.

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Research Article Tue, 10 Mar 2020 11:18:56 +0200
Arboricolonus simplex gen. et sp. nov. and novelties in Cadophora, Minutiella and Proliferodiscus from Prunus wood in Germany https://mycokeys.pensoft.net/article/46836/ MycoKeys 63: 119-161

DOI: 10.3897/mycokeys.63.46836

Authors: Steffen Bien, Ulrike Damm

Abstract: During a survey on fungi associated with wood necroses of Prunus trees in Germany, strains belonging to the Leotiomycetes and Eurotiomycetes were detected by preliminary analyses of ITS sequences. Multi-locus phylogenetic analyses (LSU, ITS, TUB, EF-1α, depending on genus) of 31 of the 45 strains from Prunus and reference strains revealed several new taxa, including Arboricolonus gen. nov., a new genus in the Helotiales (Leotiomycetes) with a collophorina-like asexual morph. Seven Cadophora species (Helotiales, Leotiomycetes) were treated. The 29 strains from Prunus belonged to five species, of which C. luteo-olivacea and C. novi-eboraci were dominating; C. africana sp. nov., C. prunicola sp. nov. and C. ramosa sp. nov. were revealed as new species. The genus Cadophora was reported from Prunus for the first time. Phialophora bubakii was combined in Cadophora and differentiated from C. obscura, which was resurrected. Asexual morphs of two Proliferodiscus species (Helotiales, Leotiomycetes) were described, including one new species, Pr. ingens sp. nov. Two Minutiella species (Phaeomoniellales, Eurotiomycetes) were detected, including the new species M. pruni-avium sp. nov. Prunus avium and P. domestica are reported as host plants of Minutiella.

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Research Article Mon, 2 Mar 2020 16:26:54 +0200
The genus Catathelasma (Catathelasmataceae, Basidiomycota) in China https://mycokeys.pensoft.net/article/36633/ MycoKeys 62: 123-138

DOI: 10.3897/mycokeys.62.36633

Authors: Zai-Wei Ge, Jian-Yun Wu, Yan-Jia Hao, Qingying Zhang, Yi-Feng An, Martin Ryberg

Abstract: Two new species, Catathelasma laorentou and C. subalpinum, are described on the basis of morphological characters, phylogenetic evidence, host preferences and geographic distributions. A taxonomic key to the known species in China is also provided to facilitate identification. Based on samples from temperate Asia, Europe and North America, the phylogeny of Catathelasma was reconstructed using the internal transcribed spacer (ITS) region, the large subunit (LSU) of the ribosomal DNA and the translation elongation factor 1-α (TEF1).The phylogenetic results showed that Catathelasma contains two monophyletic clades: the /subalpinum clade and the /imperiale clade. The Asian species C. laorentou and C. subalpinum are closely related to the North American C. sp. (labelled as C. ventricosum in GenBank) in the /subalpinum clade, whereas C. imperiale and C. singeri are closely related in the /imperiale clade.

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Research Article Mon, 3 Feb 2020 15:21:48 +0200
Integrative taxonomy confirms three species of Coniocarpon (Arthoniaceae) in Norway https://mycokeys.pensoft.net/article/48480/ MycoKeys 62: 27-51

DOI: 10.3897/mycokeys.62.48480

Authors: Andreas Frisch, Victoria Stornes Moen, Martin Grube, Mika Bendiksby

Abstract: We have studied the highly oceanic genus Coniocarpon in Norway. Our aim has been to delimit species of Coniocarpon in Norway based on an integrative taxonomic approach. The material studied comprises 120 specimens of Coniocarpon, obtained through recent collecting efforts (2017 and 2018) or received from major fungaria in Denmark, Finland, Norway and Sweden, as well as from private collectors. We have assessed (1) species delimitations and relationships based on Bayesian and maximum likelihood phylogenetic analyses of three genetic markers (mtSSU, nucITS and RPB2), (2) morphology and anatomy using standard light microscopy, and (3) secondary lichen chemistry using high-performance thin-layer chromatography. The results show three genetically distinct lineages of Coniocarpon, representing C. cinnabarinum, C. fallax and C. cuspidans comb. nov. The latter was originally described as Arthonia cinnabarina f. cuspidans and is herein raised to species level. All three species are supported by morphological, anatomical and chemical data.

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Research Article Thu, 23 Jan 2020 08:33:38 +0200
Morphology and secondary chemistry in species recognition of Parmelia omphalodes group – evidence from molecular data with notes on the ecological niche modelling and genetic variability of photobionts https://mycokeys.pensoft.net/article/38175/ MycoKeys 61: 39-74

DOI: 10.3897/mycokeys.61.38175

Authors: Emilia Ossowska, Beata Guzow-Krzemińska, Marta Kolanowska, Katarzyna Szczepańska, Martin Kukwa

Abstract: To evaluate the importance of morphological and chemical characters used in the recognition of species within the Parmelia omphalodes group, we performed phylogenetic, morphological and chemical analyses of 335 specimens, of which 34 were used for molecular analyses. Phylogenetic analyses, based on ITS rDNA sequences, show that P. pinnatifida is distinct from P. omphalodes and the most important difference between those species is the development of pseudocyphellae. In P. pinnatifida, they are mostly marginal and form white rims along lobes margins, but laminal pseudocyphellae can develop in older parts of thalli and are predominantly connected with marginal pseudocyphellae. In contrast, in P. omphalodes laminal pseudocyphellae are common and are predominantly not connected to marginal pseudocyphellae. Chemical composition of secondary lichen metabolites in both analysed species is identical and therefore this feature is not diagnostic in species recognition. Few samples of P. discordans, species morphologically similar to P. omphalodes and P. pinnatifida, were also included in the analyses and they are nested within the clade of P. omphalodes, despite the different chemistry (protocetraric acid present versus salazinic acid in P. omphalodes). All taxa of the P. omphalodes group occupy similar niches, but their potential distributions are wider than those currently known. The absence of specimens in some localities may be limited by the photobiont availability. Parmelia omphalodes and P. pinnatifida are moderately selective in photobiont choice as they form associations with at least two or three lineages of Trebouxia clade S. Parmelia pinnatifida, as well as P. discordans are associated with Trebouxia OTU S02 which seems to have a broad ecological amplitude. Other lineages of Trebouxia seem to be rarer, especially Trebouxia sp. OTU S04, which is sometimes present in P. pinnatifida. This study indicates the importance of extensive research including morphology, chemistry and analysis of molecular markers of both bionts in taxonomical studies of lichens.

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Research Article Wed, 11 Dec 2019 08:47:09 +0200
Neptunomyces aureus gen. et sp. nov. (Didymosphaeriaceae, Pleosporales) isolated from algae in Ria de Aveiro, Portugal https://mycokeys.pensoft.net/article/37931/ MycoKeys 60: 31-44

DOI: 10.3897/mycokeys.60.37931

Authors: Micael F. M. Gonçalves, Tânia F. L. Vicente, Ana C. Esteves, Artur Alves

Abstract: A collection of fungi was isolated from macroalgae of the genera Gracilaria, Enteromorpha and Ulva in the estuary Ria de Aveiro in Portugal. These isolates were characterized through a multilocus phylogeny based on ITS region of the ribosomal DNA, beta-tubulin (tub2) and translation elongation factor 1 alpha (tef1-α) sequences, in conjunction with morphological and physiological data. These analyses showed that the isolates represented an unknown fungus for which a new genus, Neptunomyces gen. nov. and a new species, Neptunomyces aureus sp. nov. are proposed. Phylogenetic analyses supported the affiliation of this new taxon to the family Didymosphaeriaceae.

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Research Article Thu, 31 Oct 2019 13:41:45 +0200
European species of Dendrostoma (Diaporthales) https://mycokeys.pensoft.net/article/37966/ MycoKeys 59: 1-26

DOI: 10.3897/mycokeys.59.37966

Authors: Walter M. Jaklitsch, Hermann Voglmayr

Abstract: European species of the genus Dendrostoma (Erythrogloeaceae, Diaporthales) occurring on Castanea sativa and Quercus spp. based on freshly collected material are presented. Using a matrix of sequences from ITS, LSU, rpb2, and tef1, five species are recognized, and their phylogenetic positions are determined. Four species are added to the 14 described species of Dendrostoma. Dendrostoma atlanticum on Castanea sativa, D. creticum on Quercus coccifera and D. istriacum on Q. ilex are described as new species, Valsa castanea is combined in Dendrostoma, and D. leiphaemia is redescribed and illustrated. A key to the European species of Dendrostoma is provided.

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Research Article Wed, 16 Oct 2019 09:13:31 +0300
Behind the veil – exploring the diversity in Phallus indusiatus s.l. (Phallomycetidae, Basidiomycota) https://mycokeys.pensoft.net/article/35324/ MycoKeys 58: 103-127

DOI: 10.3897/mycokeys.58.35324

Authors: Tiara S. Cabral, Bianca DB. Silva, María P. Martín, Charles R. Clement, Kentaro Hosaka, Iuri G. Baseia

Abstract: Studies have demonstrated that many cosmopolitan species actually consist of divergent clades that present high levels of morphological stasis throughout their evolutionary histories. Phallus indusiatus s.l. has been described as a circum-tropical species. However, this distribution may actually reflect the lack of taxonomic resolution due to the small number of diagnostic morphological characters, which leads to the identification of new records as populations of P. indusiatus. Here, we examine the diversity of P. indusiatus-like species in Brazilian Amazonia. We show a clear congruence between detailed morphological data and ITS, nuc-LSU and atp6 based phylogenetic analyses and three new species are described within the Brazilian indusiate clade. These results highlight the importance of more detailed investigation, with the inclusion of molecular information, in Neotropical fungi.

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Research Article Wed, 2 Oct 2019 08:46:06 +0300
Microsatellite based genetic diversity of the widespread epiphytic lichen Usnea subfloridana (Parmeliaceae, Ascomycota) in Estonia: comparison of populations from the mainland and an island https://mycokeys.pensoft.net/article/36557/ MycoKeys 58: 27-45

DOI: 10.3897/mycokeys.58.36557

Authors: Polina Degtjarenko, Inga Jüriado, Tiina Mandel, Tiiu Tõrra, Andres Saag, Christoph Scheidegger, Tiina Randlane

Abstract: Understanding the distribution of genetic patterns and structure is an essential target in population genetics and, thereby, important for conservation genetics. The main aim of our study was to investigate the population genetics of Usnea subfloridana, a widespread lichenised fungus, focusing on a comparison of genetic variation of its populations amongst three geographically remote and disconnected regions, in order to determine relationships amongst environmental data, variation in lichen secondary chemistry and microsatellite data in genotyped populations. In all, 928 Usnea thalli from 17 populations were genotyped using seven specific fungal microsatellite markers. Different measures of genetic diversity (allelic richness, private allelic richness, Nei’s unbiased genetic diversity and clonal diversity) were calculated and compared between lichen populations. Our results revealed a low genetic differentiation of U. subfloridana populations amongst three distant areas in Estonia and also a high level of gene flow. The results support suggestion of the long-range vegetative dispersal of subpendulous U. subfloridana via symbiotic propagules (soralia, isidia or fragments of thalli). Our study has also provided evidence that environmental variables, including mean annual temperature and geographical longitude, shape the genetic structure of U. subfloridana populations in Estonia. Additionally, a weak but statistically significant correlation between lichen chemotypes and microsatellite allele distribution was found in genotyped specimens.

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Research Article Fri, 30 Aug 2019 10:43:57 +0300
Neotypification of Protoparmeliopsis garovaglii and molecular evidence of its occurrence in Poland and South America https://mycokeys.pensoft.net/article/34501/ MycoKeys 57: 31-46

DOI: 10.3897/mycokeys.57.34501

Authors: Katarzyna Szczepańska, Pamela Rodriguez-Flakus, Jacek Urbaniak, Lucyna Śliwa

Abstract: Protoparmeliopsis garovaglii is a widely distributed placodioid lichen, which develops a distinctly rosette thallus, composed of elongated and strongly inflated to sinuous-plicate lobes. The taxon is characterised by high morphological plasticity and varied composition of secondary metabolites. However, the epithet was never typified. As such, the identity of P. garovaglii, in its strict sense, was unknown for a long time. Our phylogenetic ITS rDNA analyses, including newly generated sequences, show that European (Austria, Poland), North American (USA) and South American (Bolivia, Peru) specimens of P. garovaglii are placed in a strongly supported monophyletic clade, sister to P. muralis. We provide the first molecular evidence of the occurrence of P. garovaglii in South America (Bolivia and Peru) and the second record in Central Europe (Poland) was also provided. Furthermore, we neotypify P. garovaglii and it is reported here for the first time from Poland.

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Research Article Thu, 1 Aug 2019 10:07:23 +0300
Understanding the evolution of phenotypical characters in the Micarea prasina group (Pilocarpaceae) and descriptions of six new species within the group https://mycokeys.pensoft.net/article/33267/ MycoKeys 57: 1-30

DOI: 10.3897/mycokeys.57.33267

Authors: Beata Guzow-Krzemińska, Emmanuël Sérusiaux, Pieter P. G. van den Boom, A. Maarten Brand, Annina Launis, Anna Łubek, Martin Kukwa

Abstract: Six new Micarea species are described from Europe. Phylogenetic analyses, based on three loci, i.e. mtSSU rDNA, Mcm7 and ITS rDNA and ancestral state reconstructions, were used to evaluate infra-group divisions and the role of secondary metabolites and selected morphological characters on the taxonomy in the M. prasina group. Two main lineages were found within the group. The Micarea micrococca clade consists of twelve species, including the long-known M. micrococca and the newly described M. microsorediata, M. nigra and M. pauli. Within this clade, most species produce methoxymicareic acid, with the exceptions of M. levicula and M. viridileprosa producing gyrophoric acid. The M. prasina clade includes the newly described M. azorica closely related to M. prasina s.str., M. aeruginoprasina sp. nov. and M. isidioprasina sp. nov. The species within this clade are characterised by the production of micareic acid, with the exception of M. herbarum which lacks any detectable substances and M. subviridescens that produces prasinic acid. Based on our reconstructions, it was concluded that the ancestor of the M. prasina group probably had a thallus consisting of goniocysts, which were lost several times during evolution, while isidia and soredia evolved independently at multiple times. Our research supported the view that the ancestor of M. prasina group did not produce any secondary substances, but they were gained independently in different lineages, such as methoxymicareic acid which is restricted to M. micrococca and allied species or micareic acid present in the M. prasina clade.

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Research Article Wed, 31 Jul 2019 09:35:11 +0300
Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica https://mycokeys.pensoft.net/article/35682/ MycoKeys 55: 59-86

DOI: 10.3897/mycokeys.55.35682

Authors: Martin Vohník, Ondřej Borovec, Zuzana Kolaříková, Radka Sudová, Martina Réblová

Abstract: Seagrasses provide invaluable ecosystem services yet very little is known about their root mycobiont diversity and distribution. Here we focused on the dominant Mediterranean seagrass Posidonia oceanica and assessed its root mycobiome at 32 localities covering most of the ecoregions in the NW Mediterranean Sea using light and scanning electron microscopy and tag-encoded 454-pyrosequencing. Microscopy revealed that the recently discovered dark septate endophytic association specific for P. oceanica is present at all localities and pyrosequencing confirmed that the P. oceanica root mycobiome is dominated by a single undescribed pleosporalean fungus, hitherto unknown from other hosts and ecosystems. Its numerous slow-growing isolates were obtained from surface-sterilised root segments at one locality and after prolonged cultivation, several of them produced viable sterile mycelium. To infer their phylogenetic relationships we sequenced and analysed the large (LSU) and small (SSU) subunit nrDNA, the ITS nrDNA and the DNA-directed RNA polymerase II (RPB2). The fungus represents an independent marine biotrophic lineage in the Aigialaceae (Pleosporales) and is introduced here as Posidoniomyces atricolor gen. et sp. nov. Its closest relatives are typically plant-associated saprobes from marine, terrestrial and freshwater habitats in Southeast Asia and Central America. This study expands our knowledge and diversity of the Aigialaceae, adds a new symbiotic lifestyle to this family and provides a formal name for the dominant root mycobiont of the dominant Mediterranean seagrass.

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Research Article Wed, 26 Jun 2019 11:37:54 +0300
Elbamycella rosea gen. et sp. nov. (Juncigenaceae, Torpedosporales) isolated from the Mediterranean Sea https://mycokeys.pensoft.net/article/35522/ MycoKeys 55: 15-28

DOI: 10.3897/mycokeys.55.35522

Authors: Anna Poli, Elena Bovio, Gerard Verkley, Valeria Prigione, Giovanna Cristina Varese

Abstract: Elbamycella rosea sp. nov., introduced in the new genus Elbamycella, was collected in the Mediterranean Sea in association with the seagrass Posidonia oceanica and with the brown alga Padina pavonica. The affiliation of the new taxon to the family Juncigenaceae is supported by both morphology and phylogenetic inference based on a combined nrSSU and nrLSU sequence dataset. Maximum-likelihood and Bayesian phylogeny proved Elbamycella gen. nov. as a distinct genus within Juncigenaceae. The new genus has been compared with closely related genera and is characterised by a unique suite of characters, such as ascospores with polar appendages and peculiar shape and dimension of ascomata and asci.

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Research Article Wed, 19 Jun 2019 12:54:47 +0300
Placement of Triblidiaceae in Rhytismatales and comments on unique ascospore morphologies in Leotiomycetes (Fungi, Ascomycota) https://mycokeys.pensoft.net/article/35697/ MycoKeys 54: 99-133

DOI: 10.3897/mycokeys.54.35697

Authors: Jason M. Karakehian, Luis Quijada, Gernot Friebes, Joey B. Tanney, Donald H. Pfister

Abstract: Triblidiaceae is a family of uncommonly encountered, non-lichenized discomycetes. A recent classification circumscribed the family to include Triblidium (4 spp. and 1 subsp.), Huangshania (2 spp.) and Pseudographis (2 spp. and 1 var.). The apothecia of these fungi are persistent and drought-tolerant; they possess stromatic, highly melanized covering layers that open and close with fluctuations of humidity. Triblidialean fungi occur primarily on the bark of Quercus, Pinaceae and Ericaceae, presumably as saprobes. Though the type species of Huangshania is from China, these fungi are mostly known from collections originating from Western Hemisphere temperate and boreal forests. The higher-rank classification of triblidialean fungi has been in flux due in part to an overemphasis on ascospore morphology. Muriform ascospores are observed in species of Triblidium and in Pseudographis elatina. An intense, dark blue/purple ascospore wall reaction in iodine-based reagents is observed in species of Pseudographis. These morphologies have led, in part, to these genera being shuffled among unrelated taxa in Hysteriaceae (Dothideomycetes, Hysteriales) and Graphidaceae (Lecanoromycetes, Ostropales). Triblidiaceae has been placed within the monofamilial order Triblidiales (affinity Lecanoromycetes). Here, we demonstrate with a three-gene phylogenetic approach that triblidialean fungi are related to taxa in Rhytismatales (Leotiomycetes). We synonymize Triblidiales under Rhytismatales and emend Triblidiaceae to include Triblidium and Huangshania, with Pseudographis placed within Rhytismataceae. A history of Triblidiaceae is provided along with a description of the emended family. We discuss how the inclusion of triblidialean fungi in Rhytismatales brings some rarely observed or even unique ascospore morphologies to the order and to Leotiomycetes.

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Research Article Tue, 18 Jun 2019 09:24:07 +0300
Reassessment of the generic limits for Hydnellum and Sarcodon (Thelephorales, Basidiomycota) https://mycokeys.pensoft.net/article/35386/ MycoKeys 54: 31-47

DOI: 10.3897/mycokeys.54.35386

Authors: Karl-Henrik Larsson, Sten Svantesson, Diana Miscevic, Urmas Kõljalg, Ellen Larsson

Abstract: DNA sequences from the nuclear LSU and ITS regions were used for phylogenetic analyses of Thelephorales with a focus on the stipitate hydnoid genera Hydnellum and Sarcodon. Analyses showed that Hydnellum and Sarcodon are distinct genera but that the current division, based on basidioma texture, makes Sarcodon paraphyletic with respect to Hydnellum. In order to make genera monophyletic several species are moved from Sarcodon to Hydnellum and the following new combinations are made: Hydnellum amygdaliolens, H. fennicum, H. fuligineoviolaceum, H. fuscoindicum, H. glaucopus, H. joeides, H. lepidum, H. lundellii, H. martioflavum, H. scabrosum, H. underwoodii, and H. versipelle. Basidiospore size seems to separate the genera in most cases. Hydnellum species have basidiospore lengths in the range 4.45−6.95 µm while the corresponding range for Sarcodon is 7.4−9 µm. S. quercinofibulatus deviates from this pattern with an average spore length around 6 µm. Neotropical Sarcodon species represent a separate evolutionary lineage.

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Research Article Mon, 10 Jun 2019 09:52:24 +0300
A new species of Psathyrella (Psathyrellaceae, Agaricales) from Italy https://mycokeys.pensoft.net/article/31415/ MycoKeys 52: 89-102

DOI: 10.3897/mycokeys.52.31415

Authors: Giovanni Sicoli, Nicodemo G. Passalacqua, Antonio B. De Giuseppe, Anna Maria Palermo, Giuseppe Pellegrino

Abstract: Sporophores of a new Psathyrella species have been reported for the first time as growing at the base of Cladium mariscus culms in the Botanical Garden of the University of Calabria, Rende, Cosenza, southern Italy. The fungus was initially identified as P. thujina (= P. almerensis) by means of both ecology and macro- and microscopic characteristics of the basidiomes, then referred to P. cladii-marisci sp. nov. after extraction, amplification, purification and analysis of the rDNA ITS region. We came to this conclusion after comparing our specimen with the descriptions of the taxa available in the literature for the genus Psathyrella.

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Research Article Thu, 16 May 2019 11:33:53 +0300
How useful is the current species recognition concept for the determination of true morels? Insights from the Czech Republic https://mycokeys.pensoft.net/article/32335/ MycoKeys 52: 17-43

DOI: 10.3897/mycokeys.52.32335

Authors: Irena Petrželová, Michal Sochor

Abstract: The phylogentic diversity of the genus Morchella has only been sporadically studied in Central Europe. In this study, a molecular taxonomic revision of the Morchella species of the Czech Republic was performed using available fungarium specimens, fresh collections, and axenic cultures. Molecular phylogenetic analyses based on either ITS or five-locus (ITS, LSU, RPB1, RPB2, and EF-1α) sequencing and the application of principles of the genealogical concordance phylogenetic species recognition (GCPSR) have revealed the occurrence of 11 phylogenetic species in the region, but only six of them could be assigned unequivocally to the previously published phylospecies: Mel-3 (M. semilibera), Mel-10 (M. importuna), Mel-19 (M. eohespera), Mes-4 (M. americana), Mes-5 and Mes-8 (M. esculenta). One lineage was identified as a new phylospecies and is designated as Mel-39. Four lineages grouped together with two or more previously published phylospecies: Mel-13/26 (M. deliciosa), Mel-15/16 (M. angusticeps / M. eximioides), Mel-20/34 (M. purpurascens), and Mel-23/24/31/32 (M. pulchella). Our phylogenetic analyses and literature review shed light on the pitfalls of current molecular taxonomy of morels and highlight the ambiguities of present species recognition concepts. The main source of the problems seems to be rooted in the application of different methods (multigene vs single-gene sequencing, phenotypic determination) and approaches (monophyly vs paraphyly, the application or not of GCPSR, degree of differentiation between accepted species, etc.) by various authors for the delimitation of new phylospecies. Therefore, we propose five criteria for distinguishing new phylospecies in the genus Morchella based on molecular data, and recommend a more conservative approach in species delimitation.

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Research Article Thu, 9 May 2019 09:11:02 +0300
Looking for Lepiota psalion Huijser & Vellinga (Agaricales, Agaricaceae) https://mycokeys.pensoft.net/article/34021/ MycoKeys 52: 45-69

DOI: 10.3897/mycokeys.52.34021

Authors: Alfredo Vizzini, Alessia Tatti, Henk A. Huijser, Jun F. Liang, Enrico Ercole

Abstract: Lepiota psalion is fully described based on a recent collection from Sardinia (Italy) and the holotype. NrITS- and nrLSU-based phylogeny demonstrates that sequences deposited in GenBank as “L. psalion” and generated from two Dutch and one Chinese collections are not conspecific with the holotype and represent two distinct, undescribed species. These species are here proposed as Lepiota recondita sp. nov. and Lepiota sinorecondita ad int.

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Research Article Thu, 9 May 2019 09:10:16 +0300
Solving the taxonomic identity of Pseudotomentella tristis s.l. (Thelephorales, Basidiomycota) – a multi-gene phylogeny and taxonomic review, integrating ecological and geographical data https://mycokeys.pensoft.net/article/32432/ MycoKeys 50: 1-77

DOI: 10.3897/mycokeys.50.32432

Authors: Sten Svantesson, Karl-Henrik Larsson, Urmas Kõljalg, Tom W. May, Patrik Cangren, R. Henrik Nilsson, Ellen Larsson

Abstract: P. tristis is an ectomycorrhizal, corticioid fungus whose name is frequently assigned to collections of basidiomata as well as root tip and soil samples from a wide range of habitats and hosts across the northern hemisphere. Despite this, its identity is unclear; eight heterotypic taxa have in major reviews of the species been considered synonymous with or morphologically similar to P. tristis, but no sequence data from type specimens have been available. With the aim to clarify the taxonomy, systematics, morphology, ecology and geographical distribution of P. tristis and its morphologically similar species, we studied their type specimens as well as 147 basidiomata collections of mostly North European material. We used gene trees generated in BEAST 2 and PhyML and species trees estimated in STACEY and ASTRAL to delimit species based on the ITS, LSU, Tef1α and mtSSU regions. We enriched our sampling with environmental ITS sequences from the UNITE database. We found the P. tristis group to contain 13 molecularly and morphologically distinct species. Three of these, P. tristis, P. umbrina and P. atrofusca, are already known to science, while ten species are here described as new: P. sciastra sp. nov., P. tristoides sp. nov., P. umbrinascens sp. nov., P. pinophila sp. nov., P. alnophila sp. nov., P. alobata sp. nov., P. pluriloba sp. nov., P. abundiloba sp. nov., P. rotundispora sp. nov. and P. media sp. nov. We discovered P. rhizopunctata and P. atrofusca to form a sister clade to all other species in P. tristis s.l. These two species, unlike all other species in the P. tristis complex, are dimitic. In this study, we designate epitypes for P. tristis, P. umbrina and Hypochnopsis fuscata and lectotypes for Auricularia phylacteris and Thelephora biennis. We show that the holotype of Hypochnus sitnensis and the lectotype of Hypochnopsis fuscata are conspecific with P. tristis, but in the absence of molecular information we regard Pseudotomentella longisterigmata and Hypochnus rhacodium as doubtful taxa due to their aberrant morphology. We confirm A. phylacteris, Tomentella biennis and Septobasidium arachnoideum as excluded taxa, since their morphology clearly show that they belong to other genera. A key to the species of the P. tristis group is provided. We found P. umbrina to be a common species with a wide, Holarctic distribution, forming ectomycorrhiza with a large number of host species in habitats ranging from tropical forests to the Arctic tundra. The other species in the P. tristis group were found to be less common and have narrower ecological niches.

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Research Article Thu, 4 Apr 2019 09:18:27 +0300
Six new species of Arthrinium from Europe and notes about A. caricicola and other species found in Carex spp. hosts https://mycokeys.pensoft.net/article/32115/ MycoKeys 49: 15-48

DOI: 10.3897/mycokeys.49.32115

Authors: Ángel Pintos, Pablo Alvarado, Juan Planas, Rene Jarling

Abstract: Several new Arthrinium specimens were collected from various locations in Mediterranean and temperate Europe. A collection of the type species, A. caricicola, was obtained from dead leaves of Carex ericetorum in Berlin. Sequences of four genetic markers, ITS, 28S rDNA, tef1 and tub2 were produced from almost all collections and analyzed with those available in public databases. Results are employed to support six new species: A. balearicum, A. descalsii, A. esporlense, A. ibericum, A. italicum and A. piptatheri. The type species, A. caricicola, is related to other species occurring on Carex sp.; these might represent an independent lineage from Apiospora and the remaining species of Arthrinium. Finally, the sexual morph of A. marii is described and illustrated for the first time.

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Research Article Tue, 12 Mar 2019 13:28:50 +0200
Species identification of European forest pathogens of the genus Milesina (Pucciniales) using urediniospore morphology and molecular barcoding including M. woodwardiana sp. nov. https://mycokeys.pensoft.net/article/30350/ MycoKeys 48: 1-40

DOI: 10.3897/mycokeys.48.30350

Authors: Ben Bubner, Ramona Buchheit, Frank Friedrich, Volker Kummer, Markus Scholler

Abstract: Species of rust fungi of the genus Milesina (Pucciniastraceae, Pucciniales) are distributed mainly in northern temperate regions. They host-alternate between needles of fir (Abies spp.) and fronds of ferns (species of Polypodiales). Milesina species are distinguished based on host taxonomy and urediniospore morphology. In this study, 12 species of Milesina from Europe were revised. Specimens were examined by light and scanning electron microscopy for urediniospore morphology with a focus on visualising germ pores (number, size and position) and echinulation. In addition, barcode loci (ITS, nad6, 28S) were used for species delimitation and for molecular phylogenetic analyses. Barcodes of 72 Milesina specimens were provided, including 11 of the 12 species. Whereas urediniospore morphology features were sufficient to distinguish all 12 Milesina species except for 2 (M. blechni and M. kriegeriana), ITS sequences separated only 4 of 11 species. Sequencing with 28S and nad6 did not improve species resolution. Phylogenetic analysis, however, revealed four phylogenetic groups within Milesina that also correlate with specific urediniospore characters (germ pore number and position and echinulation). These groups are proposed as new sections within Milesina (sections Milesina, Vogesiacae M. Scholler & Bubner, sect. nov., Scolopendriorum M. Scholler & Bubner, sect. nov. and Carpaticae M. Scholler & Bubner, sect. nov.). In addition, Milesina woodwardiana Buchheit & M. Scholler, sp. nov. on Woodwardia radicans, a member of the type section Milesina, is newly described. An identification key for European Milesina species, based on urediniospore features, is provided.

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Research Article Tue, 5 Mar 2019 09:53:53 +0200
Tuber pulchrosporum sp. nov., a black truffle of the Aestivum clade (Tuberaceae, Pezizales) from the Balkan peninsula https://mycokeys.pensoft.net/article/32085/ MycoKeys 47: 35-51

DOI: 10.3897/mycokeys.47.32085

Authors: Elias Polemis, Georgios Konstantinidis, Vassiliki Fryssouli, Monica Slavova, Triantafyllos Tsampazis, Vasileios Nakas, Boris Assyov, Vasileios Kaounas, Georgios I. Zervakis

Abstract: Knowledge on the diversity of hypogeous sequestrate ascomycetes is still limited in the Balkan Peninsula. A new species of truffle, Tuber pulchrosporum, is described from Greece and Bulgaria. Specimens were collected from habitats dominated by various oak species (i.e. Quercus ilex, Q. coccifera, Q. robur) and other angiosperms. They are morphologically characterised by subglobose, ovoid to irregularly lobed, yellowish-brown to dark brown ascomata, usually with a shallow basal cavity and surface with fissures and small, dense, almost flat, trihedral to polyhedral warts. Ascospores are ellipsoid to subfusiform, uniquely ornamented, crested to incompletely reticulate and are produced in (1–)2–8-spored asci. Hair-like, hyaline to light yellow hyphae protrude from the peridium surface. According to the outcome of ITS rDNA sequence analysis, this species forms a distinct well-supported group in the Aestivum clade, with T. panniferum being the closest phylogenetic taxon.

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Research Article Wed, 20 Feb 2019 16:26:31 +0200
Description of Aeminiaceae fam. nov., Aeminium gen. nov. and Aeminium ludgeri sp. nov. (Capnodiales), isolated from a biodeteriorated art-piece in the Old Cathedral of Coimbra, Portugal https://mycokeys.pensoft.net/article/31799/ MycoKeys 45: 57-73

DOI: 10.3897/mycokeys.45.31799

Authors: João Trovão, Igor Tiago, Fabiana Soares, Diana Sofia Paiva, Nuno Mesquita, Catarina Coelho, Lídia Catarino, Francisco Gil, António Portugal

Abstract: When colonizing stone monuments, microcolonial black fungi are considered one of the most severe and resistant groups of biodeteriorating organisms, posing a very difficult challenge to conservators and biologists working with cultural heritage preservation. During an experimental survey aimed to isolate fungi from a biodeteriorated limestone art piece in the Old Cathedral of Coimbra, Portugal (a UNESCO World Heritage Site), an unknown microcolonial black fungus was retrieved. The isolated fungus was studied through a complete examination based on multilocus phylogeny of a combined dataset of ITS rDNA, LSU and rpb2, in conjunction with morphological, physiological, and ecological characteristics. This integrative analysis allows for the description of a new family, Aeminiaceae fam. nov., a new genus Aeminium gen. nov., and a new species, Aeminium ludgeri sp. nov., in the order Capnodiales.

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Research Article Mon, 28 Jan 2019 16:02:41 +0200
Bacidia albogranulosa (Ramalinaceae, lichenized Ascomycota), a new sorediate lichen from European old-growth forests https://mycokeys.pensoft.net/article/30199/ MycoKeys 44: 51-62

DOI: 10.3897/mycokeys.44.30199

Authors: Jiří Malíček, Zdeněk Palice, Jan Vondrák, Anna Łubek, Martin Kukwa

Abstract: A sterile sorediate member of the genus Bacidia s.str., B. albogranulosa, is described here as a new species. It is characterised by its very thin, pale grey thallus, white, farinose to granular soredia, the production of atranorin and the absence of ascomata and pycnidia. It grows on slightly acidic to subneutral bark of broad-leaved trees in old-growth forests in the Czech Republic, Poland, Ukraine and Russia (European part of the Caucasus). The new species is well characterised by its morphology, secondary chemistry and molecular (nrITS, mtSSU) traits. It is closely related to other atranorin-containing species in the genus, Bacidia diffracta, B. polychroa and B. suffusa.

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Research Article Fri, 14 Dec 2018 08:50:55 +0200
Additions to the taxonomy of Lagarobasidium and Xylodon (Hymenochaetales, Basidiomycota) https://mycokeys.pensoft.net/article/28987/ MycoKeys 41: 65-90

DOI: 10.3897/mycokeys.41.28987

Authors: Ilya Viner, Viacheslav Spirin, Lucie Zíbarová, Karl-Henrik Larsson

Abstract: Lagarobasidium is a small genus of wood-decaying basidiomycetes in the order Hymenochaetales. Molecular phylogenetic analyses have either supported Lagarobasidium as a distinct taxon or indicated that it should be subsumed under Xylodon, a genus that covers the majority of species formerly placed in Hyphodontia. We used sequences from the ITS and nuclear LSU regions to infer the phylogenetic position of the type species L. detriticum. Analyses confirm Lagarobasidium as a synonym of Xylodon. Molecular and morphological information show that the traditional concept of L. detriticum covers at least two species, Xylodon detriticus from Europe and X. pruinosus with known distribution in Europe and North America. Three species currently placed in Lagarobasidium are transferred to Xylodon, viz. X. magnificus, X. pumilius and X. rickii. Three new Xylodon species are described and illustrated, X. ussuriensis and X. crystalliger from East Asia and X. attenuatus from the Pacific Northwest America. The identity of X. nongravis, described from Sri Lanka, is discussed.

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Research Article Tue, 23 Oct 2018 14:04:08 +0300
The first smut fungus, Thecaphora anthemidis sp. nov. (Glomosporiaceae), described from Anthemis (Asteraceae) https://mycokeys.pensoft.net/article/28454/ MycoKeys 41: 39-50

DOI: 10.3897/mycokeys.41.28454

Authors: Julia Kruse, Volker Kummer, Roger G. Shivas, Marco Thines

Abstract: There are 63 known species of Thecaphora (Glomosporiaceae, Ustilaginomycotina), a third of which occur on Asteraceae. These smut fungi produce yellowish-brown to reddish-brown masses of spore balls in specific, mostly regenerative, plant organs. A species of Thecaphora was collected in the flower heads of Anthemis chia (Anthemideae, Asteraceae) on Rhodes Island, Greece, in 2015 and 2017, which represents the first smut record of a smut fungus on a host plant species in this tribe. Based on its distinctive morphology, host species and genetic divergence, this species is described as Thecaphora anthemidis sp. nov. Molecular barcodes of the ITS region are provided for this and several other species of Thecaphora. A phylogenetic and morphological comparison to closely related species showed that Th. anthemidis differed from other species of Thecaphora. Thecaphora anthemidis produced loose spore balls in the flower heads and peduncles of Anthemis chia unlike other flower-infecting species.

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Research Article Thu, 11 Oct 2018 10:49:29 +0300
Liberomyces pistaciae sp. nov., the causal agent of pistachio cankers and decline in Italy https://mycokeys.pensoft.net/article/28636/ MycoKeys 40: 29-51

DOI: 10.3897/mycokeys.40.28636

Authors: Salvatore Vitale, Dalia Aiello, Vladimiro Guarnaccia, Laura Luongo, Massimo Galli, Pedro W. Crous, Giancarlo Polizzi, Alessandra Belisario, Hermann Voglmayr

Abstract: A new canker and decline disease of pistachio (Pistacia vera) is described from Sicily (Italy). Observations of the disease and sampling of the causal agent started in spring 2010, in the area where this crop is typically cultivated, Bronte and Adrano (Catania province) and later extended to the Agrigento and Caltanissetta provinces. Isolations from the margins of twig, branch and stem cankers of declining plants resulted in fungal colonies with the same morphology. Pathogenicity tests on 5-year-old potted plants of Pistacia vera grafted on P. terebinthus reproduced similar symptoms to those observed in nature and the pathogen was confirmed to be a coloniser of woody plant tissue. Comparison of our isolates with the type of the apparently similar Asteromella pistaciarum showed that our isolates are morphologically and ecologically different from A. pistaciarum, the latter being a typical member of Mycosphaerellaceae. Asteromella pistaciarum is lectotypified, described and illustrated and it is considered to represent a spermatial morph of Septoria pistaciarum. Multi-locus phylogenies based on two (ITS and LSU rDNA) and three (ITS, rpb2 and tub2) genomic loci revealed isolates of the canker pathogen to represent a new species of Liberomyces within the Delonicicolaceae (Xylariales), which is here described as Liberomyces pistaciae sp. nov. (Delonicicolaceae, Xylariales). The presence of this fungus in asymptomatic plants with apparently healthy woody tissues indicates that it also has a latent growth phase. This study improves the understanding of pistachio decline, but further studies are needed for planning effective disease management strategies and ensuring that the pathogen is not introduced into new areas with apparently healthy, but infected plants.

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Research Article Tue, 18 Sep 2018 11:28:22 +0300
Multilocus phylogeny reveals taxonomic misidentification of the Schizopora paradoxa (KUC8140) representative genome https://mycokeys.pensoft.net/article/28497/ MycoKeys 38: 121-127

DOI: 10.3897/mycokeys.38.28497

Authors: Javier Fernández-López, María P. Martín, Margarita Dueñas, M. Teresa Telleria

Abstract: Schizopora paradoxa, current name Xylodon paradoxus, is a white-rot fungus with certain useful biotechnological properties. The representative genome of Schizopora paradoxa strain KUC8140 was published in 2015 as part of the 1000 Fungal Genomes Project. Multilocus phylogenetic analyses, based on three nuclear regions (ITS, LSU and rpb2), confirmed a misidentification of S. paradoxa strain KUC8140 which should be identified as Xylodon ovisporus. This wrong identification explains the unexpected geographical distribution of S. paradoxa, since this species has a European distribution, whereas the strain KUC8140 was recorded from Korea, Eastern Asia.

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Short Communication Tue, 28 Aug 2018 13:25:04 +0300
Sulcispora supratumida sp. nov. (Phaeosphaeriaceae, Pleosporales) on Anthoxanthum odoratum from Italy https://mycokeys.pensoft.net/article/27729/ MycoKeys 38: 35-46

DOI: 10.3897/mycokeys.38.27729

Authors: Indunil C. Senanayake, Rajesh Jeewon, Erio Camporesi, Kevin D. Hyde, Yu-Jia Zeng, Sheng-Li Tian, Ning Xie

Abstract: Sulcispora is typified by S. pleurospora. We collected a sulcispora-like taxon on leaves of Anthoxanthum odoratum L. in Italy and obtained single ascospore isolates. Combined ITS, LSU, SSU and tef1 sequence analyses suggested that Sulcispora is placed in the family Phaeosphaeriaceae and a newly collected Sulcispora species is introduced here as S. supratumida sp. nov. Detailed descriptions and illustrations are provided for Sulcispora supratumida and it is compared with the type species, S. pleurospora.

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Research Article Tue, 7 Aug 2018 11:25:48 +0300
Entomopathogenic fungi in Portuguese vineyards soils: suggesting a ‘Galleria-Tenebrio-bait method’ as bait-insects Galleria and Tenebrio significantly underestimate the respective recoveries of Metarhizium (robertsii) and Beauveria (bassiana) https://mycokeys.pensoft.net/article/26970/ MycoKeys 38: 1-23

DOI: 10.3897/mycokeys.38.26970

Authors: Lav Sharma, Irene Oliveira, Laura Torres, Guilhermina Marques

Abstract: Entomopathogenic fungi (EPF) are the natural enemies of insect-pests. However, EPF recoveries can be influenced by the soil habitat-type(s) incorporated and/or the bait-insect(s) used. Galleria mellonella (GM) as bait-insect, i.e. ‘Galleria-bait’, is arguably the most common methodology, which is sometimes used solely, to isolate EPF from soils. Insect baiting using Tenebrio molitor (TM) has also been employed occasionally. Here 183 soils were used to estimate the functional diversity of EPF in Portuguese Douro vineyards (cultivated habitat) and adjacent hedgerows (semi-natural habitat), using the TM bait method. Moreover, to study the effect of insect baiting on EPF recovery, 81 of these 183 soil samples were also tested for EPF occurrences using the GM bait method. Twelve species were found in 44.26% ± 3.67% of the total of 183 soils. Clonostachys rosea f. rosea was found in maximum soils (30.05% ± 3.38%), followed by Beauveria bassiana (12.57% ± 2.37%), Purpureocillium lilacinum (9.29% ± 2.14%) and Metarhizium robertsii (6.01% ± 1.75%). Beauveria pseudobassiana (P < 0.001), C. rosea f. rosea (P = 0.006) and Cordyceps cicadae (P=0.023) were isolated significantly more from hedgerows, highlighting their sensitivities towards agricultural disturbances. Beauveria bassiana (P = 0.038) and M. robertsii (P = 0.003) were isolated significantly more using GM and TM, respectively. Principal component analysis revealed that M. robertsii was associated both with TM baiting and cultivated habitats, however, B. bassiana was slightly linked with GM baiting only. Ecological profiles of B. bassiana and P. lilacinum were quite similar while M. robertsii and C. rosea f. rosea were relatively distant and distinct. To us, this is the first report on (a) C. cicadae isolation from Mediterranean soils, (b) Purpureocillium lavendulum as an EPF worldwide; and (c) significant recoveries of M. robertsii using TM over GM. Overall, a ‘Galleria-Tenebrio-bait method’ is advocated to study the functional diversity of EPF in agroecosystems.

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Research Article Mon, 6 Aug 2018 09:34:52 +0300
Neodendryphiella, a novel genus of the Dictyosporiaceae (Pleosporales) https://mycokeys.pensoft.net/article/27275/ MycoKeys 37: 19-38

DOI: 10.3897/mycokeys.37.27275

Authors: Isabel Iturrieta-González, Josepa Gené, Josep Guarro, Rafael F. Castañeda-Ruiz, Dania García

Abstract: In a survey of soil and herbivore dung microfungi in Mexico and Spain, several dendryphiella-like species were found. Phylogenetic analyses based on ITS and LSU sequences showed that these fungi belonged to the family Dictyosporiaceae (Pleosporales) and represent an undescribed monophyletic lineage distant from Dendryphiella. Therefore, the genus Neodendryphiella is proposed to accommodate three new species, N. mali, N. michoacanensis and N. tarraconensis. The novel genus shares morphological features with Dendryphiella such as differentiated conidiophores and polytretic integrated conidiogenous cells, that produce acropetal branched chains of conidia. Neodendryphiella differs in the absence of nodulose conidiophores bearing conidiogenous cells with pores surrounded by a thickened and darkened wall, typical features in the conidiogenous apparatus of Dendryphiella. In addition, the phylogenetic and morphological analysis of several reference strains of different Dendryphiella species, available for comparison, support the proposal of D. variabilis sp. nov., which mainly differs from the other species of the genus by having conidia up to 7 septa and highlight that D. vinosa and D. infuscans are obscure species that require further taxonomic review.

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Research Article Thu, 26 Jul 2018 08:52:32 +0300
A new species of Rhodocybe sect. Rufobrunnea (Entolomataceae, Agaricales) from Italy https://mycokeys.pensoft.net/article/27094/ MycoKeys 36: 21-33

DOI: 10.3897/mycokeys.36.27094

Authors: Alfredo Vizzini, Renato Jonny Ferrari, Enrico Ercole, Alessandro Fellin

Abstract: Rhodocybe fumanellii is described from Italy as a new species based both on morphological and molecular nrITS/nrLSU data. It belongs in sect. Rufobrunnea and is characterised by massive tricholomatoid basidiomata with reddish-brown tinges, adnate and crowded lamellae, an enlarged stipe base with long rhizomorphs, long sinuose slender cheilocystidia, ellipsoid basidiospores and the presence of caulocystidia. Drawings of the main micromorphological features as well as a colour photograph of fresh basidiomata in situ are provided and its morphological relationships with allied species are discussed.

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Research Article Tue, 10 Jul 2018 08:57:25 +0300
Different diversification histories in tropical and temperate lineages in the ascomycete subfamily Protoparmelioideae (Parmeliaceae) https://mycokeys.pensoft.net/article/22548/ MycoKeys 36: 1-19

DOI: 10.3897/mycokeys.36.22548

Authors: Garima Singh, Francesco Dal Grande, Jan Schnitzler, Markus Pfenninger, Imke Schmitt

Abstract: Background: Environment and geographic processes affect species’ distributions as well as evolutionary processes, such as clade diversification. Estimating the time of origin and diversification of organisms helps us understand how climate fluctuations in the past might have influenced the diversification and present distribution of species. Complementing divergence dating with character evolution could indicate how key innovations have facilitated the diversification of species. Methods: We estimated the divergence times within the newly recognised subfamily Protoparmelioideae (Ascomycota) using a multilocus dataset to assess the temporal context of diversification events. We reconstructed ancestral habitats and substrate using a species tree generated in *Beast. Results: We found that the diversification in Protoparmelioideae occurred during the Miocene and that the diversification events in the tropical clade Maronina predate those of the extratropical Protoparmelia. Character reconstructions suggest that the ancestor of Protoparmelioideae was most probably a rock-dwelling lichen inhabiting temperate environments. Conclusions: Major diversification within the subtropical/tropical genus Maronina occurred between the Paleocene and Miocene whereas the diversifications within the montane, arctic/temperate genus Protoparmelia occurred much more recently, i.e. in the Miocene.

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Research Article Mon, 2 Jul 2018 11:59:58 +0300
Short-spored Subulicystidium (Trechisporales, Basidiomycota): high morphological diversity and only partly clear species boundaries https://mycokeys.pensoft.net/article/25678/ MycoKeys 35: 41-99

DOI: 10.3897/mycokeys.35.25678

Authors: Alexander Ordynets, David Scherf, Felix Pansegrau, Jonathan Denecke, Ludmila Lysenko, Karl-Henrik Larsson, Ewald Langer

Abstract: Diversity of corticioid fungi (resupinate Basidiomycota), especially outside the northern temperate climatic zone, remains poorly explored. Furthermore, most of the known species are delimited by morphological concepts only and, not rarely, these concepts are too broad and need to be tested by molecular tools. For many decades, the delimitation of species in the genus Subulicystidium (Hydnodontaceae, Trechisporales) was a challenge for mycologists. The presence of numerous transitional forms as to basidiospore size and shape hindered species delimitation and almost no data on molecular diversity have been available. In this study, an extensive set of 144 Subulicystidium specimens from Paleo- and Neotropics was examined. Forty-nine sequences of ITS nuclear ribosomal DNA region and 51 sequences of 28S nuclear ribosomal DNA region from fruit bodies of Subulicystidium were obtained and analysed within the barcoding gap framework and with phylogenetic Bayesian and Maximum likelihood approaches. Eleven new species of Subulicystidium are described based on morphology and molecular analyses: Subulicystidium boidinii, S. fusisporum, S. grandisporum, S. harpagum, S. inornatum, S. oberwinkleri, S. parvisporum, S. rarocrystallinum, S. robustius, S. ryvardenii and S. tedersooi. Morphological and DNA-evidenced borders were revised for the five previously known species: S. naviculatum, S. nikau, S. obtusisporum, S. brachysporum and S. meridense. Species-level variation in basidiospore size and shape was estimated based on systematic measurements of 2840 spores from 67 sequenced specimens. An updated identification key to all known species of Subulicystidium is provided.

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Research Article Wed, 27 Jun 2018 09:49:00 +0300
Diversity of polypores in the Dominican Republic: Pseudowrightoporia dominicana sp. nov. (Hericiaceae, Russulales) https://mycokeys.pensoft.net/article/25371/ MycoKeys 34: 35-45

DOI: 10.3897/mycokeys.34.25371

Authors: Alfredo Vizzini, Claudio Angelini, Cristiano Losi, Enrico Ercole

Abstract: The new species Pseudowrightoporia dominicana is described from the Dominican Republic based on morphological and molecular data (nrITS and nrLSU sequence analyses). It is mainly characterised by pileate basidiomata with a bright pinkish context and a di-trimitic hyphal system. Phylogenetically, it is sister to the African species P. gillesii and to the Asiatic P. japonica.

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Research Article Wed, 16 May 2018 08:58:47 +0300
A new species of Stamnaria (Leotiomycetes, Helotiales) from Western Siberia https://mycokeys.pensoft.net/article/23277/ MycoKeys 32: 49-63

DOI: 10.3897/mycokeys.32.23277

Authors: Danny Haelewaters, Nina V. Filippova, Hans-Otto Baral

Abstract: A new species of Stamnaria is described based on morphology and molecular data from a collection made in West Siberia. Stamnaria yugrana is differentiated by lanceolate, strongly protruding paraphyses and comparatively narrow, fusoid-clavate ascospores. The apothecia are urn-shaped due to a prominent and even collar as in S. persoonii. The species grows on fallen side branches of Equisetum sylvaticum, a rarely recorded host for Stamnaria. The authors formally describe the new species and provide colour illustrations. In addition, the literature is reviewed on previously described species of Stamnaria. Phylogenetic reconstruction of the Stamnaria lineage, based on the ITS ribosomal DNA, strongly supports the three currently recognised species: S. americana, S. persoonii and S. yugrana.

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Research Article Tue, 20 Mar 2018 10:06:32 +0200
A multi-gene phylogeny of Chlorophyllum (Agaricaceae, Basidiomycota): new species, new combination and infrageneric classification https://mycokeys.pensoft.net/article/23831/ MycoKeys 32: 65-90

DOI: 10.3897/mycokeys.32.23831

Authors: Zai-Wei Ge, Adriaana Jacobs, Else C. Vellinga, Phongeun Sysouphanthong, Retha van der Walt, Carmine Lavorato, Yi-Feng An, Zhu L. Yang

Abstract: Taxonomic and phylogenetic studies of Chlorophyllum were carried out on the basis of morphological differences and molecular phylogenetic analyses. Based on the phylogeny inferred from the internal transcribed spacer (ITS), the partial large subunit nuclear ribosomal DNA (nrLSU), the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-α (tef1) sequences, six well-supported clades and 17 phylogenetic species are recognised. Within this phylogenetic framework and considering the diagnostic morphological characters, two new species, C. africanum and C. palaeotropicum, are described. In addition, a new infrageneric classification of Chlorophyllum is proposed, in which the genus is divided into six sections. One new combination is also made. This study provides a robust basis for a more detailed investigation of diversity and biogeography of Chlorophyllum.

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Research Article Tue, 20 Mar 2018 08:54:49 +0200
The lichens of the Alps – an annotated checklist https://mycokeys.pensoft.net/article/23568/ MycoKeys 31: 1-634

DOI: 10.3897/mycokeys.31.23568

Authors: Pier Luigi Nimis, Josef Hafellner, Claude Roux, Philippe Clerc, Helmut Mayrhofer, Stefano Martellos, Peter O. Bilovitz

Abstract: This is the first attempt to provide an overview of the lichen diversity of the Alps, one of the biogegraphically most important and emblematic mountain systems worldwide. The checklist includes all lichenised species, plus a set of non- or doubtfully lichenised taxa frequently treated by lichenologists, excluding non-lichenised lichenicolous fungi. Largely based on recent national or regional checklists, it provides a list of all infrageneric taxa (with synonyms) hitherto reported from the Alps, with data on their distribution in eight countries (Austria, France, Germany, Liechtenstein, Monaco, Italy, Slovenia, Switzerland) and in 42 Operational Geographic Units, mostly corresponding to administrative subdivisions within the countries. Data on the main substrates and on the altitudinal distribution are also provided. A short note points to the main ecological requirements of each taxon and/or to open taxonomic problems. Particularly poorly known taxa are flagged and often provided with a short description, to attract the attention of specialists. The total number of infrageneric taxa is 3,163, including 117 non- or doubtfully lichenised taxa. The richness of the lichen biota fairly well corresponds with the percent of the Alpine area occupied by each country: Austria (2,337 taxa), Italy (2,169), France (2,028), Switzerland (1,835), Germany (1,168), Slovenia (890) and Lichtenstein (152), no lichen having ever been reported from Monaco. The number of poorly known taxa is quite high (604, 19.1% of the total), which indicates that, in spite of the Alps being one of the lichenologically most studied mountain systems worldwide, much work is still needed to reach a satisfactory picture of their real lichen diversity. Thirteen new combinations are proposed in the genera Agonimia, Aspicilia, Bagliettoa, Bellemerea, Carbonea, Lepra, Miriquidica, Polysporina, Protothelenella, Pseudosagedia and Thelidium.

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Monograph Mon, 12 Mar 2018 16:18:01 +0200
Three new species of Fomitiporella (Hymenochaetales, Basidiomycota) based on the evidence from morphology and DNA sequence data https://mycokeys.pensoft.net/article/23109/ MycoKeys 30: 73-89

DOI: 10.3897/mycokeys.30.23109

Authors: Xiao-Hong Ji, Josef Vlasák, Xue-Mei Tian, Yu-Cheng Dai

Abstract: Fomitiporella austroasiana, F. mangrovei and F. vietnamensis are described and illustrated as new species based on morphological characters and molecular evidence. They have annual to perennial, mostly resupinate basidiomata with grayish fresh pores, an indistinct subiculum, lack any kind of setae, have brownish, thick-walled basidiospores, and cause a white rot. The distinctive morphological characters of the new species and their related species are discussed. Phylogenies based on the nuclear ribosomal large subunit (28S) and the nuclear ribosomal ITS region show that these three new species form three distinct lineages in the Fomitiporella clade. A key to known species of Fomitiporella is given.

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Research Article Thu, 8 Mar 2018 11:51:07 +0200
An overview of the genus Coprotus (Pezizales, Ascomycota) with notes on the type species and description of C. epithecioides sp. nov. https://mycokeys.pensoft.net/article/22978/ MycoKeys 29: 15-47

DOI: 10.3897/mycokeys.29.22978

Authors: Ivana Kušan, Neven Matočec, Margita Jadan, Zdenko Tkalčec, Armin Mešić

Abstract: In a mycological research performed in the Sjeverni Velebit National Park, Croatia, a new species of Coprotus was discovered, described here as C. epithecioides. Along with the microscopic examination, phylogenetic analysis of the type material, based on ITS and LSU sequences, was performed in order to evaluate its relationship with the type species, C. sexdecimsporus. The type species was sequenced in this study for the first time, providing ITS and LSU sequences from two separate collections which displayed differences in macroscopic characters and content of paraphyses. An extended description of C. sexdecimsporus based on Croatian material is also provided. A worldwide identification key to the species assigned to the genus Coprotus is presented, along with a species overview, containing a data matrix. The phylogenetic position of Coprotus in the Boubovia-Coprotus clade within Pyronemataceae s.l. is discussed. Coprotus sexdecimsporus is also reported here as new to the Croatian mycobiota.

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Research Article Fri, 12 Jan 2018 11:36:30 +0200
Taxonomic novelties and new records of Fennoscandian crustose lichens https://mycokeys.pensoft.net/article/13375/ MycoKeys 25: 51-86

DOI: 10.3897/mycokeys.25.13375

Authors: Måns Svensson, Stefan Ekman, Jon T. Klepsland, Anders Nordin, Göran Thor, Gesa von Hirschheydt, Fredrik Jonsson, Tommy Knutsson, Mattias Lif, Toby Spribille, Martin Westberg

Abstract: We present taxonomic, distributional and ecological notes on Fennoscandian crustose lichens and lichenicolous fungi, based on new collections as well as revision of herbarium material. Two new combinations are proposed: Frutidella furfuracea comb. nov. for F. pullata and Puttea duplex comb. nov. for Fellhanera duplex. Lecidea byssoboliza, L. carneoglauca and Variolaria torta are all reduced to synonymy with Bacidia antricola, Bacidia invertens is synonymized with B. igniarii, B. atrolivida with Mycobilimbia tetramera, and Gyalidea fruticola with Thelenella pertusariella. A new description is provided for Micarea hylocomii. 25 species of lichens and lichenicolous fungi are reported as new to Finland, Norway and/or Sweden: Absconditella lignicola (Norway), Bacidia antricola (Norway), B. polychroa (Norway), B. pycnidiata (Sweden), Bacidina adastra (Sweden), Biatora veteranorum (Norway), Briancoppinsia cytospora (Finland), Catillaria scotinodes (Norway), Cliostomum subtenerum (Norway), Dirina fallax (Sweden), Fellhaneropsis almquistiorum (Norway), Gyalidea subscutellaris (Sweden), Lecania inundata (Norway), L. suavis (Norway), Micarea capitata (Norway), M. deminuta (Norway), M. hylocomii (Sweden), M. lynceola (Sweden), M. soralifera (Sweden), M. subconfusa (Sweden), Mycoblastus sanguinarioides (Finland, Sweden), Paralecia pratorum (Sweden), Puttea duplex (Sweden), Sarcogyne algoviae (Finland) and Toninia subnitida (Norway). Lectotypes are designated for Bacidia antricola, Lecidea byssoboliza, Lecidea carneoglauca, Lecidea subconfusa and Lecidea submoestula.

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Research Article Mon, 10 Jul 2017 08:50:08 +0300
Unexpected high species diversity among European stalked puffballs – a contribution to the phylogeny and taxonomy of the genus Tulostoma (Agaricales) https://mycokeys.pensoft.net/article/12176/ MycoKeys 21: 33-88

DOI: 10.3897/mycokeys.21.12176

Authors: Mikael Jeppson, Alberto Altes, Gabriel Moreno, R. Henrik Nilsson, Yolanda Loarce, Alfredo de Bustos, Ellen Larsson

Abstract: A three-gene data set was generated to explore species diversity and delimitations within the stalked puffballs (Tulostoma, Agaricales) in Europe. Data on species from other parts of the world were included for comparison of species concepts and distribution ranges. Sequence data from 26 type specimens are included. The phylogenetic analyses support Tulostoma as monophyletic. Eleven major clades, 37 minor clades, and 20 single branches were recovered and found to correspond to 30 described species and 27 species without scientific names. Five species are here described as new to science: Tulostoma calcareum, T. calongei, T. eckbladii, T. grandisporum, and T. pannonicum. In total we report 26 described, and 19 undescribed, species from Europe. An epitype for T. fimbriatum with ITS sequence data is selected to fix the name. The recovered tree topology was not in congruence with the current infrageneric classification of Tulostoma, suggesting that many of the morphological characters used for segregation of taxa are plesiomorphic or homoplasious. Spore ornamentation and hyphal structure of the peridium are found to be reliable characters for delimitation of species. The majority of the species occur in the dry, arid areas of southern and east central Europe but a few are shown to be restricted to humid temperate regions in the North. The study confirms that species with smooth or sub-smooth spores are restricted to dry and arid habitats whereas species with more strongly ornamented spores occur in humid habitats. Areas with steppe vegetation in Hungary and Spain are here identified as hot spots for Tulostoma species diversity.

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Research Article Mon, 24 Apr 2017 15:26:15 +0300
What do we learn from cultures in the omics age? High-throughput sequencing and cultivation of leaf-inhabiting endophytes from beech (Fagus sylvatica L.) revealed complementary community composition but similar correlations with local habitat conditions https://mycokeys.pensoft.net/article/11265/ MycoKeys 20: 1-16

DOI: 10.3897/mycokeys.20.11265

Authors: Abu Bakar Siddique, Anis Mahmud Khokon, Martin Unterseher

Abstract: Comparative simultaneous studies of environmental high-throughput sequencing (HTS) and cultivation of plant-associated fungi have rarely been conducted in the past years. For the present contribution, HTS and extinction culturing were applied for the same leaf samples of European beech (Fagus sylvatica) in order to trace both “real” environmental drivers as well as method-dependent signals of the observed mycobiomes. Both approaches resulted in non-overlapping community composition and pronounced differences in taxonomic classification and trophic stages. However, both methods revealed similar correlations of the fungal communities with local environmental conditions. Our results indicate undeniable advantages of HTS over cultivation in terms of revealing a good representation of the major functional guilds, rare taxa and biodiversity signals of leaf-inhabiting fungi. On the other hand our results demonstrate that the immense body of literature about cultivable endophytic fungi can and should be used for the interpretation of community signals and environmental correlations obtained from HTS studies and that cultivation studies should be continued at the highest standards, e.g. when sequencing facilities are not available or if such surveys are expanded into functional aspects with experiments on living isolates.

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Research Article Tue, 21 Feb 2017 09:28:39 +0200
Polypores and genus concepts in Phanerochaetaceae (Polyporales, Basidiomycota) https://mycokeys.pensoft.net/article/10153/ MycoKeys 17: 1-46

DOI: 10.3897/mycokeys.17.10153

Authors: Otto Miettinen, Viacheslav Spirin, Josef Vlasák, Bernard Rivoire, Soili Stenroos, David Hibbett

Abstract: We explored whether DNA-phylogeny-based and morphology-based genus concepts can be reconciled in the basidiomycete family Phanerochaetaceae. Our results show that macromorphology of fruiting bodies and hymenophore construction do not reflect monophyletic groups. However, by integrating micromorphology and re-defining genera, harmonization of DNA phylogeny and morphological genus concepts is possible in most cases. In the case of one genus (Phlebiopsis), our genetic markers could not resolve genus limits satisfactorily and a clear morphological definition could not be identified. We combine extended species sampling, microscopic studies of fruiting bodies and phylogenetic analyses of ITS, nLSU and rpb1 to revise genus concepts. Three new polypore genera are ascribed to the Phanerochaetaceae: Oxychaete gen. nov. (type Oxyporus cervinogilvus), Phanerina gen. nov. (type Ceriporia mellea), and Riopa (including Ceriporia metamorphosa and Riopa pudens sp. nov.). Phlebiopsis is extended to include Dentocorticium pilatii, further species of Hjortstamia and the monotypic polypore genus Castanoporus. The polypore Ceriporia inflata is combined into Phanerochaete. The identity of the type species of the genus Riopa, R. davidii, has been misinterpreted in the current literature. The species has been included in Ceriporia as a species of its own or placed in synonymy with Ceriporia camaresiana. The effort to properly define R. davidii forced us to study Ceriporia more widely. In the process we identified five closely related Ceriporia species that belong to the true Ceriporia clade (Irpicaceae). We describe those species here, and introduce the Ceriporia pierii group. We also select a lectotype and an epitype for Riopa metamorphosa and neotypes for Sporotrichum aurantiacum and S. aurantium, the type species of the anamorphic genus Sporotrichum, and recommend that teleomorphic Riopa is conserved against it.

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Research Article Thu, 8 Dec 2016 10:30:49 +0200
High habitat-specificity in fungal communities in oligo-mesotrophic, temperate Lake Stechlin (North-East Germany) https://mycokeys.pensoft.net/article/9646/ MycoKeys 16: 17-44

DOI: 10.3897/mycokeys.16.9646

Authors: Christian Wurzbacher, Norman Warthmann, Elizabeth Bourne, Katrin Attermeyer, Martin Allgaier, Jeff R. Powell, Harald Detering, Susan Mbedi, Hans-Peter Grossart, Michael T. Monaghan

Abstract: Freshwater fungi are a poorly studied ecological group that includes a high taxonomic diversity. Most studies on aquatic fungal diversity have focused on single habitats, thus the linkage between habitat heterogeneity and fungal diversity remains largely unexplored. We took 216 samples from 54 locations representing eight different habitats in the meso-oligotrophic, temperate Lake Stechlin in North-East Germany. These included the pelagic and littoral water column, sediments, and biotic substrates. We performed high throughput sequencing using the Roche 454 platform, employing a universal eukaryotic marker region within the large ribosomal subunit (LSU) to compare fungal diversity, community structure, and species turnover among habitats. Our analysis recovered 1027 fungal OTUs (97% sequence similarity). Richness estimates were highest in the sediment, biofilms, and benthic samples (189–231 OTUs), intermediate in water samples (42–85 OTUs), and lowest in plankton samples (8 OTUs). NMDS grouped the eight studied habitats into six clusters, indicating that community composition was strongly influenced by turnover among habitats. Fungal communities exhibited changes at the phylum and order levels along three different substrate categories from littoral to pelagic habitats. The large majority of OTUs (> 75%) could not be classified below the order level due to the lack of aquatic fungal entries in public sequence databases. Our study provides a first estimate of lake-wide fungal diversity and highlights the important contribution of habitat heterogeneity to overall diversity and community composition. Habitat diversity should be considered in any sampling strategy aiming to assess the fungal diversity of a water body.

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Research Article Fri, 21 Oct 2016 14:43:07 +0300
The Flora Mycologica Iberica Project fungi occurrence dataset https://mycokeys.pensoft.net/article/9765/ MycoKeys 15: 59-72

DOI: 10.3897/mycokeys.15.9765

Authors: Francisco Pando, Margarita Dueñas, Carlos Lado, María Teresa Telleria

Abstract: The dataset contains detailed distribution information on several fungal groups. The information has been revised, and in many times compiled, by expert mycologist(s) working on the monographs for the Flora Mycologica Iberica Project (FMI). Records comprise both collection and observational data, obtained from a variety of sources including field work, herbaria, and the literature. The dataset contains 59,235 records, of which 21,393 are georeferenced. These correspond to 2,445 species, grouped in 18 classes. The geographical scope of the dataset is Iberian Peninsula (Continental Portugal and Spain, and Andorra) and Balearic Islands. The complete dataset is available in Darwin Core Archive format via the Global Biodiversity Information Facility (GBIF).

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Data Paper Tue, 13 Sep 2016 11:29:58 +0300
Oomycete-specific ITS primers for identification and metabarcoding https://mycokeys.pensoft.net/article/9244/ MycoKeys 14: 17-30

DOI: 10.3897/mycokeys.14.9244

Authors: Taavi Riit, Leho Tedersoo, Rein Drenkhan, Eve Runno-Paurson, Harri Kokko, Sten Anslan

Abstract: Microbial metabarcoding studies using high throughput sequencing technologies generate unprecedented amounts of DNA sequence data and make it possible to determine not only the composition of the communities but also the underlying factors powering the evolution of these communities. Despite the potential of community level studies in helping to better understand the ecology of pathogens and to manage the losses caused by them, very few oomycete addressing metabarcoding studies have been carried out and with highly variable results. The aim of this study was to develop new oomycete-specific ITS region PCR primers with improved specificity for metabarcoding and identification of oomycetes. The modified ITS1oo and the newly developed ITS3oo primers show improved in silico specificity for oomycetes and when paired with the universal ITS4 successfully amplified the DNA from all eleven tested oomycete species from six genera. High throughput sequencing of 20 soil samples from forest nurseries and bordering areas, using the primer pair ITS1oo/ITS4, recovered more than 400 oomycete OTUs, which is a significant increase over previous studies, and indicates the ability of the new method to detect various oomycete groups from complex substrates. The average fraction of oomycete reads per soil samples was 32–36%, with a maximum of 69%. The recovered oomycete OTUs represented the groups Lagenidiales, Peronosporales, Pythiales and Saprolegniales, with Pythiales dominating in all samples. In addition, the new primers were successfully used in identifying pathogens directly from infected plant tissues with Sanger sequencing. The pathogen was identified to the species or genus level in four samples out of six. In conclusion, the developed oomycete-specific primers provide a reliable method for the identification and metabarcoding of oomycetes.

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Research Article Wed, 31 Aug 2016 15:01:34 +0300
Detection of signal recognition particle (SRP) RNAs in the nuclear ribosomal internal transcribed spacer 1 (ITS1) of three lineages of ectomycorrhizal fungi (Agaricomycetes, Basidiomycota) https://mycokeys.pensoft.net/article/8579/ MycoKeys 13: 21-33

DOI: 10.3897/mycokeys.13.8579

Authors: Magnus Alm Rosenblad, María P. Martín, Leho Tedersoo, Martin K. Ryberg, Ellen Larsson, Christian Wurzbacher, Kessy Abarenkov, R. Henrik Nilsson

Abstract: During a routine scan for Signal Recognition Particle (SRP) RNAs in eukaryotic sequences, we surprisingly found in silico evidence in GenBank for a 265-base long SRP RNA sequence in the ITS1 region of a total of 11 fully identified species in three ectomycorrhizal genera of the Basidiomycota (Fungi): Astraeus, Russula, and Lactarius. To rule out sequence artifacts, one specimen from a species indicated to have the SRP RNA-containing ITS region in each of these genera was ordered and re-sequenced. Sequences identical to the corresponding GenBank entries were recovered, or in the case of a non-original but conspecific specimen differed by three bases, showing that these species indeed have an SRP RNA sequence incorporated into their ITS1 region. Other than the ribosomal genes, this is the first known case of non-coding RNAs in the eukaryotic ITS region, and it may assist in the examination of other types of insertions in fungal genomes.

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Research Article Fri, 13 May 2016 09:59:17 +0300
New records of lichenized and lichenicolous fungi in Scandinavia https://mycokeys.pensoft.net/article/6670/ MycoKeys 11: 33-61

DOI: 10.3897/mycokeys.11.6670

Authors: Martin Westberg, Einar Timdal, Johan Asplund, Mika Bendiksby, Reidar Haugan, Fredrik Jonsson, Per Larsson, Göran Odelvik, Mats Wedin, Ana M. Millanes

Abstract: Fourteen species of lichenized or lichenicolous fungi are reported new to either Norway or Sweden or both countries. Several of these are rare and almost unknown. The reported species are: Acarospora insignis (new to Norway), A. pyrenopsoides (Norway, Sweden), A. versicolor (Norway), Calvitimela perlata (Sweden), Lecidea degeliana (Sweden), Nephroma helveticum (Sweden), Peltula placodizans (Norway), Phacographa protoparmeliae (Norway), Rhizocarpon pycnocarpoides (Norway, Sweden), Sarcogyne algoviae (Sweden), Sarcogyne hypophaeoides (Norway, Sweden), Tephromela grumosa (Norway), Tremella lobariacearum (Norway) and Tremella wirthii (Sweden). In addition Cladonia albonigra is confirmed from Sweden. Sarcogyne hypophaeoides is lectotypified and is also reported new to Austria.

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Research Article Fri, 13 Nov 2015 01:22:04 +0200
Transatlantic disjunction in fleshy fungi III: Gymnopus confluens https://mycokeys.pensoft.net/article/4700/ MycoKeys 9: 37-63

DOI: 10.3897/mycokeys.9.4700

Authors: Karen W. Hughes, Ronald H. Petersen

Abstract: Phylogeographic data indicate that DNA differences consistently exist between the North American and European allopatric populations of Gymnopus confluens. Conversely, pairing experiments show that collections from both populations were sexually compatible in vitro and detailed morphological examinations of numerous fresh and dried basidiomata do not produce qualitative differences. Percent ITS sequence divergence between Europe and North American collections of G. confluens was 3.25%. Species delineation metrics including Rosenberg’s PAB statistic, PID metrics, RRD (randomly distributed) and PTP (Poisson Tree Processes) gave mixed indications that North American and European populations were distinct at species rank. The North American populations are described as Gymnopus confluens subsp. campanulatus (Peck) R.H. Petersen.

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Research Article Fri, 3 Apr 2015 13:21:58 +0300
Buwchfawromyces eastonii gen. nov., sp. nov.: a new anaerobic fungus (Neocallimastigomycota) isolated from buffalo faeces https://mycokeys.pensoft.net/article/4799/ MycoKeys 9: 11-28

DOI: 10.3897/mycokeys.9.9032

Authors: Tony Martin Callaghan, Sabine Marie Podmirseg, Daniel Hohlweck, Joan Elizabeth Edwards, Anil Kumar Puniya, Sumit Singh Dagar, Gareth Wyn Griffith

Abstract: The novel anaerobic fungus Buwchfawromyces eastonii gen. nov., sp. nov., belonging to order Neocallimastigales (phylum Neocallimastigomycota) is described. Morphologically similar to Piromyces but genetically quite distinct, this fungus (isolate GE09) was first isolated from buffalo faeces in west Wales and then subsequently isolated from sheep, cattle and horse in the same area. Phylogenetic analysis of LSU and ITS sequence confirmed that B. eastonii isolates formed a distinct clade close to the polycentric Anaeromyces spp. The morphology of GE09 is monocentric with monoflagellate zoospores. However, the sporangial stalk (sporangiophore) is often distinctly swollen and the proximal regions of the rhizoidal system twisted in appearance.

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Research Article Wed, 4 Mar 2015 00:00:00 +0200
Tremella rhizocarpicola sp. nov. and other interesting lichenicolous Tremellales and Filobasidiales in the Nordic countries https://mycokeys.pensoft.net/article/4068/ MycoKeys 8: 31-41

DOI: 10.3897/mycokeys.8.8176

Authors: Ana Millanes, Paul Diederich, Martin Westberg, Tommy Knutsson, Mats Wedin

Abstract: New data on the diversity and geographical distribution of lichenicolous Tremellales and Filobasidiales in the Nordic countries is presented. One new species, Tremella rhizocarpicola, is formally described. Tremella pertusariae and T. protoparmeliae are reported as new to the Nordic countries, Syzygospora physciacearum is new to Iceland, Tremella rinodinae is new to Sweden, and T. caloplacae is new to Norway. Nine species are reported as new to a number of Swedish provinces, including Biatoropsis usnearum, Syzygospora bachmannii, S. physciacearum, Tremella caloplacae, T. cetrariicola, T. cladoniae, T. coppinsii, T. diploschistina, and T. hypogymniae.

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Research Article Tue, 16 Sep 2014 00:00:00 +0300
DNA barcoding and morphological studies reveal two new species of waxcap mushrooms (Hygrophoraceae) in Britain https://mycokeys.pensoft.net/article/1198/ MycoKeys 7: 45-62

DOI: 10.3897/mycokeys.7.5860

Authors: Antony Ainsworth, Paul Cannon, Bryn Dentinger

Abstract: Rigorous diagnostics and documentation of fungal species are fundamental to their conservation. During the course of a species-level study of UK waxcap (Hygrophoraceae) diversity, two previously unrecognized species were discovered. We describe Gliophorus europerplexus sp. nov. and G. reginae sp. nov., respectively orange–brown and purple–pink waxcap mushrooms, from nutrient-poor grasslands in Britain. Both share some morphological features with specimens assigned to Gliophorus (=Hygrocybe) psittacinus. However, analysis of sequences of the nuclear ITS DNA barcode region from these and related taxa confirms the phylogenetic distinctness of these lineages. Furthermore, we demonstrated that the holotype of Hygrophorus perplexus, a North American species morphologically resembling G. europerplexus, is phylogenetically divergent from all our collections. It is likely that further collections of G. europerplexus will be revealed by sequencing European material currently filed under G. perplexus and its synonyms. However, two such collections in the Kew fungarium yielded sequences that clustered together but were divergent from those of G. europerplexus, G. perplexus and G. psittacinus and may represent a further novel taxon. By contrast, G. reginae is morphologically distinct and can usually be recognized in the field by its purplish viscid pileus and relatively stout, flexuose, pale stipe. It is named to commemorate the diamond jubilee of Her Majesty Queen Elizabeth II in 2012 and the 60th anniversary of her coronation in 2013.

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Research Article Mon, 9 Sep 2013 00:00:00 +0300
Cortinarius bovarius (Agaricales), a new species from western North America https://mycokeys.pensoft.net/article/1200/ MycoKeys 7: 23-30

DOI: 10.3897/mycokeys.7.5182

Authors: Kare Liimatainen, Tuula Niskanen

Abstract: Cortinarius bovarius sp. nov., a conifer associated taxon growing on calcareous ground, is described from western North America. Phylogenetic relationships and species limits were investigated using rDNA ITS and nuclear rpb2 sequences, morphological and ecological data. The species belongs to section Bovini and its closest relative is European C. bovinus.

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Research Article Tue, 14 May 2013 00:00:00 +0300
DNA barcode identification of lichen-forming fungal species in the Rhizoplaca melanophthalma species-complex (Lecanorales, Lecanoraceae), including five new species https://mycokeys.pensoft.net/article/1201/ MycoKeys 7: 1-22

DOI: 10.3897/mycokeys.7.4508

Authors: Steven Leavitt, Fernando Fernández-Mendoza, Sergio Pérez-Ortega, Mohammad Sohrabi, Pradeep Divakar, Thorsten Lumbsch, Larry St. Clair

Abstract: Recent studies using sequence data from multiple loci and coalescent-based species delimitation have revealed several species-level lineages within the phenotypically circumscribed taxon Rhizoplaca melanophthalma sensu lato. Here, we formally describe five new species within this group, R. occulta, R. parilis, R. polymorpha, R. porterii, and R. shushanii, using support from the coalescent-based species delimitation method implemented in the program Bayesian Phylogenetics and Phylogeography (BPP) as the diagnostic feature distinguishing new species. We provide a reference DNA sequence database using the ITS marker as a DNA barcode for identifying species within this complex. We also assessed intraspecific genetic distances within the six R. melanophthalma sensu lato species. While intraspecific genetic distances within the five new species were less than or equal to the lowest interspecific pairwise comparison values, an overlap in genetic distances within the R. melanophthalma sensu stricto clade suggests the potential for additional phenotypically cryptic lineages within this broadly distributed lineage. Overall, our results demonstrate the potential for accurately identifying species within the R. melanophthalma group by using molecular-based identification methods.

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Research Article Thu, 9 May 2013 00:00:00 +0300
Biogeography and ecology of Cetraria aculeata, a widely distributed lichen with a bipolar distribution https://mycokeys.pensoft.net/article/1195/ MycoKeys 6: 33-53

DOI: 10.3897/mycokeys.6.3185

Authors: Christian Printzen, Stephanie Domaschke, Fernando Fernández-Mendoza, Sergiо Pérez-Ortega

Abstract: Ecological and historical biogeography of lichens have rarely been studied in a concerted effort, but both aspects have to be taken into consideration when explaining the distributional patterns of species. This review summarizes, partly preliminary, results from a series of studies on phylogeography, ecophysiology and symbiotic interactions of the lichen Cetraria aculeata. This species is not only widespread but also occupies a very wide ecological niche. Evidence suggests that Cetraria aculeata has evolved and diversified in the Northern Hemisphere and colonised the Southern Hemisphere from there. Genetic isolation of populations indicates the absence of ongoing long range dispersal and genetic exchange between geographically isolated populations. We observe a hitherto unrecognized genetic diversity that may indicate ecotypic differentiation and speciation processes. Mediterranean and Polar populations differ not only genetically, but also in ecophysiological properties. Ongoing common garden experiments will have to show whether genetically fixed adaptation or acclimation is responsible for these differences. The genetic structure of the photobiont is best explained by climatic differences between localities, but co-dispersal with the mycobiont plays an important role as well. Taken together, these results indicate that a photobiont switch in the past enabled C. aculeata to widen its ecological niche, with subsequent genetic isolation of populations. Photobiont switches may play a crucial role in speciation processes of lichens. A combination of ecophysiological and phylogeographic studies with experimental approaches is necessary to better understand the reaction of lichens to changing environmental conditions.

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Review Article Tue, 23 Apr 2013 00:00:00 +0300
Five species of Candelaria and Candelariella (Ascomycota, Candelariales) new to Switzerland https://mycokeys.pensoft.net/article/1185/ MycoKeys 3: 1-12

DOI: 10.3897/mycokeys.3.2864

Authors: Martin Westberg, Philippe Clerc

Abstract: Candelaria pacifica, Candelariella antennaria, C. boleana, C. granuliformis and C. xanthostigmoides are reported from Switzerland for the first time. Candelariella xanthostigmoides is also new to Europe. Candelariella aggregata, C. efflorescens, C. subdeflexa and C. viae-lactea are confirmed to occur in Switzerland. Candelariella antennaria is also reported new to Austria. Brief notes on their identification, ecology and distribution in Switzerland are given.

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Research Article Fri, 30 Mar 2012 00:00:00 +0300