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Abstract

Introduction

A word on the query and reference datasets

Guideline 1. It is simple to check that all query sequences represent the ITS region

Guideline 2. A single alignment step can assess the orientation of the query sequences

Guideline 3. PCR chimeras tend to lack full counterparts in the sequence databases and are therefore usually easy to spot through BLAST

Guideline 4. Sequences can be broken in other, puzzling ways; BLAST, again, will tell

Guideline 5. Taxonomic annotations should be verified before the sequences are used

Taking action on bad sequences in INSD / UNITE

Concluding remarks

Acknowledgements

References

Appendix