Research Article |
Corresponding author: Jiwen Xia ( zhenjunxue@126.com ) Academic editor: Nalin Wijayawardene
© 2022 Zhaoxue Zhang, Rongyu Liu, Shubin Liu, Taichang Mu, Xiuguo Zhang, Jiwen Xia.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Zhang Z, Liu R, Liu S, Mu T, Zhang X, Xia J (2022) Morphological and phylogenetic analyses reveal two new species of Sporocadaceae from Hainan, China. MycoKeys 88: 171-192. https://doi.org/10.3897/mycokeys.88.82229
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Species of Sporocadaceae have often been reported as plant pathogens, endophytes or saprophytes and are commonly isolated from a wide range of plant hosts. The isolated fungi were studied through a complete examination, based on multilocus phylogenies from combined datasets of ITS/tub2/tef1, in conjunction with morphological characteristics. Nine strains were isolated from Ficus microcarpa, Ilex chinensis and Schima superba in China which represented four species, viz., Monochaetia schimae sp. nov., Neopestalotiopsis haikouensis sp. nov., Neopestalotiopsis piceana and Pestalotiopsis licualicola. Neopestalotiopsis piceana was a new country record for China and first host record from Ficus macrocarpa. Pestalotiopsis licualicola was first report from Ilex chinensis in China.
Monochaetia, multigene phylogeny, Neopestalotiopsis, Pestalotiopsis
The family Sporocadaceae was established by Corda in 1842 (type genus: Sporocadus). Species of Sporocadaceae are endophytic, plant pathogenic or saprobic, and associated with a wide range of host plants (
To date, most phylogenetic studies addressing genera of Sporocadaceae have been based solely on ITS and LSU sequences (
The samples were collected from Hainan Province, China. The strains were isolated from diseased leaves of Ficus microcarpa, Ilex chinensis and Schima superba using surface disinfected tissue fragments (0.5 × 0.5 cm) taken from the margin of leaf lesions (
Genomic DNA was extracted from fungal mycelium grown on PDA using cetyltrimethylammonium bromide (CTAB) protocol as described in
PCR was performed using an Eppendorf Master Thermocycler (Hamburg, Germany). Amplification reactions were performed in a 50 μL reaction volume, which contained 25 μL Green Taq Mix (Vazyme, Nanjing, China), 2 μL of each forward and reverse primer (10 μM) (Tsingke, Beijing, China), and 2 μL template genomic DNA, to which distilled deionized water was added. PCR parameters were as follows: 94 °C for 5 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at a suitable temperature for 30 s, extension at 72 °C for 1 min and a final elongation step at 72 °C for 7 min. Annealing temperature was 55 °C for ITS, 54 °C for tub2, 52 °C for tef1. The PCR products were visualised on 1% agarose electrophoresis gel. Sequencing was done bi-directionally, conducted by the Tsingke Biotechnology Company Limited (Qingdao, China). Consensus sequences were obtained using MEGA 7.0 or MEGA-X (
Newly generated sequences in this study were aligned with additional related sequences downloaded from GenBank (Table
Species and GenBank accession numbers of DNA sequences used in this study. New sequences are in bold.
Species | Strain | Host/substrate | Country | GenBank accession number | Reference | ||
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ITS | tef1 | tub2 | |||||
Bartalinia robillardoides | CBS 122705 T | Leptoglossus occidentalis | Italy | LT853104 | LT853202 | LT853252 |
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Ciliochorella phanericola | MFLUCC 14-0984 T | Phanera purpurea | Thailand | KX789680 | – | KX789682 |
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MFLUCC 12-0310 | Phanera purpurea | Thailand | KF827444 | KF827477 | KF827478 |
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Monochaetia castaneae | CFCC 54354 = SM9-1 T | Castanea mollissima | China | MW166222 | MW199741 | MW218515 |
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SM9-2 | Castanea mollissima | China | MW166223 | MW199742 | MW218516 |
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M. dimorphospora | NBRC 9980 | Castanea pubinervis | Japan | LC146750 | – | – |
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M. ilicis | KUMCC 15-0520 T | Ilex sp. | China | KX984153 | – | – |
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CBS 101009 | Air | Japan | MH553953 | MH554371 | MH554612 |
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M. junipericola | CBS 143391 T | Juniperus communis | Germany | MH107900 | MH108021 | MH108045 |
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M. kansensis | PSHI2004Endo1030 | Cyclobalaopsis myrsinaefolia | China | DQ534044 | – | DQ534047 | Liu et al. 2006 |
PSHI2004Endo1031 | Cyclobalaopsis myrsinaefolia | China | DQ534045 | – | DQ534048 | Liu et al. 2006 | |
M. monochaeta | CBS 546.80 | Culture contaminant | Netherlands | MH554056 | MH554491 | MH554732 |
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CBS 199.82 T | Quercus pubescens | Italy | MH554018 | – | MH554694 |
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CBS 115004 | Quercus robur | Netherlands | AY853243 | MH554398 | MH554639 |
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M. quercus | CBS 144034 T | Quercus eduardi | Mexico | MH554171 | MH554606 | MH554844 |
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M. schimae | SAUCC212201 T | Schima superba | China | MZ577565 | OK104874 | OK104867 | This study |
SAUCC212202 | Schima superba | China | MZ577566 | OK104875 | OK104868 | This study | |
SAUCC212203 | Schima superba | China | MZ577567 | OK104876 | OK104869 | This study | |
M. sinensis | HKAS 10065 T | Quercus sp. | China | MH115995 | – | MH115999 |
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Neopestalotiopsis acrostichi | MFLUCC 17-1754 T | Acrostichum aureum | Thailand | MK764272 | MK764316 | MK764338 |
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N. alpapicalis | MFLUCC 17-2544 T | Rhizophora mucronata | Thailand | MK357772 | MK463547 | MK463545 |
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N. aotearoa | CBS 367.54 T | Canvas | New Zealand | KM199369 | KM199526 | KM199454 |
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N. asiatica | MFLUCC 12-0286 T | Unidentified tree | China | JX398983 | JX399049 | JX399018 |
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CFCC 54339 = SM32 | Castanea mollissima | China | MW166224 | MW199743 | MW218517 |
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N. brachiata | MFLUCC 17-1555 T | Rhizophora apiculata | Thailand | MK764274 | MK764318 | MK764340 |
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N. brasiliensis | COAD 2166 T | Psidium guajava | Brazil | MG686469 | MG692402 | MG692400 |
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CFCC 54341 = ZY4 | Castanea mollissima | China | MW166229 | MW199748 | MW218522 |
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ZY4-2D | Castanea mollissima | China | MW166230 | MW199749 | MW218523 |
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N. chiangmaiensis | MFLUCC 18-0113 T | Dead leaves | Thailand | – | MH388404 | MH412725 |
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N. chrysea | MFLUCC 12-0261 T | Pandanus sp. | China | JX398985 | JX399051 | JX399020 |
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N. clavispora | MFLUCC 12-0281 T | Magnolia sp. | China | JX398979 | JX399045 | JX399014 |
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N. cocoes | MFLUCC 15-0152 T | Cocos nucifera | Thailand | KX789687 | KX789689 | – | |
N. coffea-arabica | HGUP 4019 T | Coffea arabica | China | KF412649 | KF412646 | KF412643 |
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N. cubana | CBS 600.96 T | Leaf litter | Cuba | KM199347 | KM199521 | KM199438 |
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N. dendrobii | MFLUCC 14-0106 T | Dendrobium cariniferum | Chiang Rai, Thailand | MK993571 | MK975829 | MK975835 |
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N. egyptiaca | CBS 140162 T | Mangifera indica | Egypt | KP943747 | KP943748 | KP943746 |
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N. ellipsospora | MFLUCC 12-0283 T | Dead plant materials | China | JX398980 | JX399047 | JX399016 |
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N. eucalypticola | CBS 264.37 T | Eucalyptus globulus | – | KM199376 | KM199551 | KM199431 |
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N. foedans | CGMCC 3.9123 T | Mangrove plant | China | JX398987 | JX399053 | JX399022 |
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N. formicidarum | CBS 362.72 T | Dead ant | Ghana | KM199358 | KM199517 | KM199455 |
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CBS 115.83 | Plant debris | Cuba | KM199344 | KM199519 | KM199444 |
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N. hadrolaeliae | COAD 2637 T | Hadrolaelia jongheana | Minas Gerais, Brazil | MK454709 | MK465122 | MK465120 |
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N. haikouensis | SAUCC212271 T | Ilex chinensis | China | OK087294 | OK104877 | OK104870 | This study |
SAUCC212272 | Ilex chinensis | China | OK087295 | OK104878 | OK104871 | This study | |
N. honoluluana | CBS 114495 T | Telopea sp. | USA | KM199364 | KM199548 | KM199457 |
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N. iraniensis | CBS 137768 T | Fragaria ananassa | Iran | KM074048 | KM074051 | KM074057 | Ayoubi et al. 2016 |
N. javaensis | CBS 257.31 T | Cocos nucifera | Indonesia | KM199357 | KM199543 | KM199437 |
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N. macadamiae | BRIP 63737c T | Macadamia integrifolia | Australia | KX186604 | KX186627 | KX186654 |
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N. magna | MFLUCC 12-0652 T | Pteridium sp. | France | KF582795 | KF582791 | KF582793 |
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N. mesopotamica | CBS 336.86 T | Pinus brutia | Iraq | KM199362 | KM199555 | KM199441 |
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N. musae | MFLUCC 15-0776 T | Musa sp. | Thailand | KX789683 | KX789685 | KX789686 | |
N. natalensis | CBS 138.41 T | Acacia mollissima | South Africa | KM199377 | KM199552 | KM199466 |
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N. pandanicola | KUMCC 17-0175 T | Pandanaceae | China | – | MH388389 | MH412720 |
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N. pernambucana | URM 7148-01 T | Vismia guianensis | Brazil | KJ792466 | KU306739 | – | |
N. petila | MFLUCC 17-1738 T | Rhizophora mucronata | Thailand | MK764276 | MK764320 | MK764342 |
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N. phangngaensis | MFLUCC 18-0119 T | Pandanaceae | Thailand | MH388354 | MH388390 | MH412721 |
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N. piceana | CBS 394.48 T | Picea sp. | UK | KM199368 | KM199527 | KM199453 |
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CBS 254.32 | Cocos nucifera | Indonesia | KM199372 | KM199529 | KM199452 |
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SAUCC210112 | Ficus microcarpa | China | OK149224 | OK206436 | OK206434 | This study | |
SAUCC210113 | Ficus microcarpa | China | OK149225 | OK206437 | OK206435 | This study | |
N. protearum | CBS 114178 T | Leucospermum cuneiforme cv. “Sunbird” | Zimbabwe | JN712498 | KM199542 | KM199463 |
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N. rhizophorae | MFLUCC 17-1550 T | Rhizophora mucronata | Thailand | MK764278 | MK764322 | MK764344 |
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N. rosae | CBS 124745 | Paeonia suffruticosa | USA | KM199360 | KM199524 | KM199430 |
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CBS 101057 T | Rosa sp. | New Zealand | KM199359 | KM199523 | KM199429 |
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N. rosicola | CFCC 51992 T | Rosa chinensis | China | KY885239 | KY885243 | KY885245 | |
CFCC 51993 | Rosa chinensis | China | KY885240 | KY885244 | KY885246 | N |
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N. samarangensis | MFLUCC 12-0233 T | Syzygium samarangense | Thailand | JQ968609 | JQ968611 | JQ968610 |
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N. saprophytica | MFLUCC 12-0282 T | Magnolia sp. | China | KM199345 | KM199538 | KM199433 |
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N. sichuanensis | CFCC 54338 = SM15-1 T | Castanea mollissima | China | MW166231 | MW199750 | MW218524 |
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N. sonneratae | MFLUCC 17-1745 T | Sonneronata alba | Thailand | MK764280 | MK764324 | MK764346 |
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N. steyaertii | IMI 192475 T | Eucalytpus viminalis | Australia | KF582796 | KF582792 | KF582794 |
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N. surinamensis | CBS 450.74 T | soil under Elaeis guineensis | Suriname | KM199351 | KM199518 | KM199465 |
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N. thailandica | MFLUCC 17-1730 T | Rhizophora mucronata | Thailand | MK764281 | MK764325 | MK764347 |
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N. umbrinospora | MFLUCC 12-0285 T | unidentified plant | China | JX398984 | JX399050 | JX399019 |
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N. vitis | MFLUCC 15-1265 T | Vitis vinifera cv. “Summer black” | China | KU140694 | KU140676 | KU140685 |
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N. zimbabwana | CBS 111495 T | Leucospermum cunciforme cv. “Sunbird” | Zimbabwe | JX556231 | KM199545 | KM199456 |
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Nonappendiculata quercina | CBS 116061 T | Quercus suber | Italy | MH553982 | MH554400 | MH554641 |
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CBS 270.82 | Quercus pubescens | Italy | MH554025 | MH554459 | MH554701 |
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Pestalotiopsis australasiae | CBS 114126 T | Knightia sp. | New Zealand | KM199297 | KM199499 | KM199409 |
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P. australis | CBS 114193 T | Grevillea sp. | Australia | KM199332 | KM199475 | KM199383 |
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P. grevilleae | CBS 114127 T | Grevillea sp. | Australia | KM199300 | KM199504 | KM199407 |
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P. hollandica | CBS 265.33 T | Sciadopitys verticillata | The Netherlands | KM199328 | KM199481 | KM199388 |
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P. kenyana | CBS 442.67 T | Coffea sp. | Kenya | KM199302 | KM199502 | KM199395 |
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P. knightiae | CBS 114138 T | Knightia sp. | New Zealand | KM199310 | KM199497 | KM199408 |
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P. licualicola | HGUP4057 T | Licuala grandis | China | KC492509 | KC481684 | KC481683 |
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SAUCC210087 | Ilex chinensis | China | OK087323 | OK104879 | OK104872 | This study | |
SAUCC210088 | Ilex chinensis | China | OK087324 | OK104880 | OK104873 | This study | |
P. oryzae | CBS 353.69 T | Oryza sativa | Denmark | KM199299 | KM199496 | KM199398 |
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P. parva | CBS 278.35 | Leucothoe fontanesiana | – | KM199313 | KM199509 | KM199405 |
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P. portugalica | CBS 393.48 T | – | Portugal | KM199335 | KM199510 | KM199422 |
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P. spathuliappendiculata | CBS 144035 T | Phoenix canariensis | Australia | MH554172 | MH554607 | MH554845 |
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Pseudopestalotiopsis cocos | CBS 272.29 T | Cocos nucifera | Indonesia | KM199378 | KM199553 | KM199467 |
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Pse. elaeidis | CBS 413.62 T | Elaeis guineensis | Nigeria | MH554044 | MH554479 | MH554720 |
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Pse. indica | CBS 459.78 T | Rosa sinensis | India | KM199381 | KM199560 | KM199470 |
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Seiridium papillatum | CBS 340.97 T | Eucalyptus delegatensis | Australia | LT853102 | MH554468 | LT853250 |
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Seir. phylicae | CBS 133587 T | Phylica arborea | Tristan da Cunha | LT853091 | LT853188 | LT853238 |
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Nine strains of Sporocadaceae isolated from plant hosts from Hainan, China, were grown in culture and used for analyses of molecular sequence data. The combined dataset of ITS-tub2-tef1 has an aligned length of 2285 total characters (ITS: 1–638, tub2: 639–1558, tef1: 1559–2285) including gaps, of which 869 characters are constant, 292 variable and parsimony-uninformative, and 1124 parsimony-informative. For the BI and ML analyses, the substitution model GTR+G for ITS, HKY+I+G for tub2 and GTR+I+G for tef1 were selected and incorporated into the analyses. The MCMC analysis of the three concatenated genes run for 7,795,000 generations, resulting in 7796 trees. The ML tree topology confirmed the tree topologies obtained from the BI analyses, and therefore, only the ML tree is presented (Fig.
Phylogram of Sporocadaceae based on combined ITS, tub2 and tef1 sequences. The BI and ML bootstrap support values above 0.90 and 70% are shown at the first and second position, respectively. The tree is rooted to Bartalinia robillardoides (CBS 122705), ex-type or ex-epitype cultures are indicated in bold face. Strains from the current study are in red. Some branches were shortened according to the indicated mulipliers.
Bayesian posterior probability (≥ 0.90) and ML bootstrap support values (≥ 70%) are shown as first and second position above nodes. The 96 strains were assigned to 75 species clades based on the three gene loci phylogeny (Fig.
China, Hainan Province: East Harbour National Nature Reserve, on diseased leaves of Schima superba, 23 May 2021, Z.X. Zhang (holotype HSAUP212201; ex-type living culture SAUCC212201).
Name refers to the genus of the host plant Schima superba.
Leaf spots irregular, pale brown in centre, brown to tan at margin. Sexual morph not observed. Asexual morph on PDA: Conidiomata solitary, scattered, black, raising above surface of culture medium, subglobose, exuding black conidial droplets from central ostioles after 10 days in light at 25 °C. Conidiophores cylindrical, hyaline, smooth-walled. Conidiogenous cells 9.0–16.5 × 1.2–2.2 μm, phialidic, ampulliform, discrete, hyaline, smooth, thin-walled. Conidia 18–24 × 4.5–6.0 μm, mean ± SD = 20.5 ± 1.1 × 5.5 ± 0.4 μm, fusiform, tapering at both ends, 4-septate; apical cell 2.0–4.0 μm long, conical, hyaline and smooth-walled; three median cells doliiform, 12.5–15.5 μm long, mean ± SD = 14.2 ± 0.7 μm, olivaceous, rough-walled, upper second cell 3.8–5.3 μm long, upper third cell 3.4–5.0 μm long, upper fourth cell 4.4–5.4 μm long; basal cell 2.2–4.5 μm long, conical, hyaline and smooth-walled; apical appendage 7.0–12.5 μm long (mean = 9.2 μm), single, unbranched, central, tubular, filiform; basal appendage 2.5–5.0 μm long, single, unbranched tubular, filiform.
Colonies on PDA 39.0–45.0 mm in diameter after 15 days at 25 °C in darkness, growth rate 2.5–3.0 mm/day, irregularly circular, raised, dense surface with lobate edge, zonate in different sectors, light brown at the margin, brown at the centre; reverse brown at the margin, dark brown at the centre.
China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of Schima superba, paratype HSAUP212202, living culture SAUCC212202; on diseased leaves of Schima superba, paratype HSAUP212203, living culture SAUCC212203.
Monochaetia schimae is introduced based on the multi-locus phylogenetic analysis, with three isolates clustering separately in a well-supported clade (BI/ML = 0.99/96). Monochaetia schimae is phylogenetically close to M. castaneae from leaves of Castanea mollissima, M. ilicis from leaves of Ilex sp., and M. junipericola from twigs of Juniperus communis. However, Monochaetia schimae differs from M. castaneae by 148 nucleotides (11/463 in ITS, 89/743 in tub2 and 48/403 in tef1), from M. ilicis by 94 nucleotides (18/526 in ITS, 32/698 in tub2 and 44/456 in tef1), and from M. junipericola by 91 nucleotides (10/524 in ITS, 40/411 in tub2 and 41/304 in tef1). Furthermore, they are distinguished by hosts and conidial sizes (18.0–24.0 × 4.5–6.0 μm in M. schimae vs. 18.8–27.3 × 4.7–6.6 μm in M. castaneae vs. 20.0–27.0 × 5.0–8.0 μm in M. ilicis vs. 22.0–28.0 × 5.0–7.0 μm in M. junipericola). In morphology, Monochaetia castaneae differs from M. schimae by the colour of colonies (cinnamon vs. brown), Monochaetia ilicis differs from M. schimae by the colour of median cells (brown vs. olivaceous), and M. junipericola differs from M. schimae by longer conidiogenous cells (10.0–30.0 μm vs. 9.0–16.5 μm) (
China, Hainan Province, Haikou City: East Harbour National Nature Reserve, on diseased leaves of Ilex chinensis. 23 May 2021, Z.X. Zhang (holotype HSAUP212271; ex-type living culture SAUCC212271).
Named after the host location, Haikou City.
Leaf spots irregular, grey white in centre, brown to tan at margin. Sexual morph not observed. Asexual morph on PDA: Conidiomata globose to clavate, solitary or confluent, embedded or semi-immersed to erumpent, dark brown, exuding globose, dark brown to black conidial masses. Conidiophores indistinct, often reduced to conidiogenous cells. Conidiogenous cells discrete, subcylindrical to ampulliform, hyaline, 5.0–10.0 × 2.0–6.0 μm, apex 1.0–2.0 μm diam. Conidia fusoid, ellipsoid, straight to slightly curved, 4-septate, 16.0–22.0 × 4.5–7.0 μm, mean ± SD = 20.0 ± 1.8 × 5.5 ± 0.4 μm; basal cell conical with a truncate base, hyaline, rugose and thin-walled, 3.0–4.5 μm long; three median cells doliiform, 11.5–15.0 μm long, mean ± SD = 13.2 ± 1.0 μm, wall rugose, septa darker than the rest of the cell, second cell from the base pale brown, 3.5–5.5 μm long; third cell honey-brown, 4.0–6.0 μm long; fourth cell brown, 3.8–5.7 μm long; apical cell 2.5–5.5 μm long, hyaline, cylindrical to subcylindrical, thin- and smooth-walled; with 2–3 tubular apical appendages (mostly 3), arising from the apical crest, unbranched, filiform, 13.5–24.0 μm long, mean ± SD = 19.1 ± 3.5 μm; basal appendage 2.0–7.0 μm long, single, tubular, unbranched, centric.
Colonies on PDA occupying an entire 90 mm petri dish in 7 days at 25 °C in darkness, growth rate of 7.0–14.0 mm/day, edge undulate, white to grey white, with moderate aerial mycelium on the surface, with black, gregarious conidiomata; reverse similar in colour.
China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of Ilex chinensis, paratype HSAUP212272, living culture SAUCC212272.
Phylogenetic analysis of a combined three-gene ITS-tub2-tef1 showed that Neopestalotiopsis haikouensis formed an independent clade with full-supported (BI/ML = 1/100, Fig.
Leaf spots irregular, pale brown in centre, brown to tan at margin. Asexual morph on PDA: Conidiomata solitary, globose to clavate, semi-immersed, brown to black; exuding globose, dark brown to black conidial masses. Conidiophores reduced to conidiogenous cells. Conidiogenous cells discrete, ampulliform to lageniform, hyaline, smooth and thin walled, simple, 4.0–12.0 × 2.0–10.0 μm, apex 2.0–5.0 μm diam. Conidia ellipsoid to clavate, straight to slightly curved, 4-septate, 19.5–26.5 × 5.5–7.0 μm, mean ± SD = 22.7 ± 0.8 × 6.1 ± 0.4 μm; somewhat constricted at septa; basal cell obconic with truncate base, rugose and thin-walled, 2.7–5.0 μm long; three median cells 12.0–16.0 μm long, mean ± SD = 14.7 ± 0.9 μm, doliiform, verruculose, versicoloured, septa darker than the rest of the cell, second cell from base pale brown, 4.0–5.7 μm long; third cell dark brown, 3.5–5.2 μm long; fourth cell brown, 3.8–5.8 μm long; apical cell obconic, hyaline, thin and smooth-walled, 2.5–5.2 μm long; with 1–3 tubular apical appendages, arising from the apical crest, flexuous, unbranched, 21.0–32.0 μm long, mean ± SD = 24.8 ± 3.5 μm; basal appendage single, tubular, unbranched, centric, 2.7–6.5 μm long.
Colonies on PDA incubated at 25 °C in the dark with an average radial growth rate of 9.0–14.0 mm/day and occupying an entire 90 mm petri dish in 7 d, with edge undulate, whitish, aerial mycelium on surface, fruiting bodies black, concentric; reverse of culture yellow to pale brown.
China, Hainan Province: Five Fingers Group Scenic Area, 20 May 2021, Z.X. Zhang. On diseased leaves of Ficus microcarpa, HSAUP210112, living culture SAUCC210112; on diseased leaves of Ficus microcarpa, HSAUP210113, living culture SAUCC210113.
In the present study, two strains (SAUCC210112 and SAUCC210113) from symptomatic leaves of Ficus microcarpa were clustered with Neopestalotiopsis piceana clade (
Leaf spots irregular, pale brown in centre, brown to tan at margin. Asexual morph on PDA: Conidiomata solitary, scattered, black, raising above surface of culture medium, subglobose. Conidiophores cylindrical, hyaline, smooth-walled. Conidiophores often indistinct. Conidiogenous cells discrete, hyaline, simple, filiform, 5.5–10.0 μm long. Conidia 18.0–24.5 × 4.0–5.5 μm, mean ± SD = 20.5 ± 1.9 × 5.3 ± 0.3 μm, fusiform, straight to slightly curved, 4-septate, smooth, greyish brown; basal cell conical, hyaline, thin-walled, 2.8–6.0 μm long; with three median cells, dark brown, concolorous, septa and periclinal walls darker than the rest of the cell, together 11.5–16.0 μm long, mean ± SD = 13.2 ± 1.2 μm; second cell from base 3.4–5.5 μm; third cell 3.3–4.7 μm; fourth cell 3.5–5.1 μm; apical cell hyaline, conic to subcylindrical, 3.1–5.3 μm; with 1–3 tubular apical appendages (mostly 1) without knobs, arising from the apex of the apical cell, 10.0–20.5 μm long, mean ± SD = 16.0 ± 4.0 μm; basal appendage filiform, short.
Colonies on PDA reaching 70.0–80.0 mm diam after 7 d at 25 °C, growth rate 9.0–12.0 mm/day, edge entire, whitish to pale honey coloured, with sparse aerial mycelium on the surface, with black, gregarious conidiomata; reverse similar in colour.
China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of Ilex chinensis, HSAUP210087, living culture SAUCC210087; on diseased leaves of Ilex chinensis, HSAUP210088, living culture SAUCC210088.
In the present study, two strains (SAUCC210087 and SAUCC210088) from symptomatic leaves of Ilex chinensis were clustered to Pestalotiopsis licualicola clade (
Based on phylogeny and morphology, nine strains from three host species (Ficus microcarpa, Ilex chinensis and Schima superba) were described as well as two new species (Monochaetia schimae sp. nov. and Neopestalotiopsis haikouensis sp. nov.) and two known species (Neopestalotiopsis piceana and Pestalotiopsis licualicola). In the genus Monochaetia, most species were found on Fagaceae hosts, including Castanea pubinervis (Monochaetia dimorphospora), Castanea mollissima (Monochaetia castaneae), Quercus pubescens (Monochaetia monochaeta) and etc. In our study, the species of Monochaetia (M. schimae) was first reported from Schima superba (Theaceae). Ilex was widely grown as an evergreen tree all over the world and isolated many pathogens, endophytes or saprophytes (
As many pestalotioid species have overlapping morphological traits, sequence data is essential to resolve these three genera and introduce new species (
This work was jointly supported by the National Natural Science Foundation of China (nos. 31900014, U2002203, 31750001) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100704).
The combined ITS, tub2 and tef1 sequences
Data type: phylogenetic
Explanation note: The combined ITS, tub2 and tef1 sequences.