Research Article |
Corresponding author: Li Fan ( fanli@mail.cnu.edu.cn ) Academic editor: Rui-Lin Zhao
© 2022 Ning Mao, Jing-Chong Lv, Yu-Yan Xu, Tao-Yu Zhao, Li Fan.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Mao N, Lv J-C, Xu Y-Y, Zhao T-Y, Fan L (2022) Two new Clitocella species from North China revealed by phylogenetic analyses and morphological characters. MycoKeys 88: 151-170. https://doi.org/10.3897/mycokeys.88.80068
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Two new species of Clitocella are proposed based on morphological and phylogenetic investigations. Clitocella borealichinensis sp. nov. is closely related to C. orientalis but distinguished from the latter by its slightly smaller basidiospores and hyphae of pileipellis with pale brown to brown intracellular or parietal pigment. Clitocella colorata sp. nov. is closely related to C. popinalis and C. mundula in macromorphology but is differentiated from C. popinalis by its slightly smaller basidiospores and the difference in genetic profile, and from C. mundula by its relatively colorful pileus (white to yellowish white, grayish white to grayish brown, pink white). Phylogenetic analyses based on sequence data from five different loci (ITS, nrLSU, tef1, rpb2 and atp6) support the taxonomic position of the two new species in the genus Clitocella. The illustrations and descriptions for the new taxa are provided.
Entolomataceae, multigene, phylogeny, taxonomy
The genus Clitocella Kluting, T.J. Baroni & Bergemann (Entolomataceae, Agaricales), with C. popinalis (Fr.) Kluting, T.J. Baroni & Bergemann as the type species, was established in 2014 (
In China, the species diversity of Clitocella is scarce and only two species are described (
Collections were obtained and photographed in the field from Shanxi regions in China, and then dried in a fruit drier at 40–50 °C and deposited in BJTC herbarium (Capital Normal University, Beijing, China). The sizes of basidiomata (pileal width) used in this study are as follows: small: <30 mm; medium-sized: 30–50 mm; large: >50 mm. Standardised color values were obtained from ColorHexa (http://www.colorhexa.com/). Microscopic characters were observed in sections obtained from dry specimens mounted in 3% KOH, Congo Red, or Melzer’s reagent (
Dried basidiomata were crushed by shaking for 45 s at 30 Hz 2–4 times (Mixer Mill MM301, Retsch, Haan, Germany) in a 1.5 mL tube together with a 3 mm diam tungsten carbide ball. Total genomic DNA was extracted from the powdered basidiomata using NuClean Plant Genomic DNA Kit (CWBIO, China), following the manufacturer’s instructions. Primers ITS1F and ITS4 were employed for the ITS (
PCR reactions were implemented as follows: an initial denaturation at 94 °C for 5 min, then to 35 cycles of the following denaturation at 94 °C for 30 s, annealing at 52 °C for 45 s (ITS), 60 s (nrLSU), 72 °C for 1 min; and a final extension at 72 °C for 10 min. Amplification of rpb2 and tef1 sequences followed
Specimens used in molecular phylogenetic studies and their GenBank accession numbers. Newly generated sequences are in bold.
Species | Voucher | Locality | GenBank accession No. | ||||
---|---|---|---|---|---|---|---|
ITS | nrLSU | rpb2 | tef1 | atp6 | |||
Catathelasma ventricosum | DAOM221514 | USA | KP255469 | – | – | – | – |
Clitocella colorata | BJTC FM1593 | China | OL966940 | – | – | – | – |
Clitocella colorata | BJTC FM1594 | China | OL966941 | – | – | – | – |
Clitocella colorata | BJTC FM1891 | China | OL966944 | OL966955 | OL989914 | OL989918 | OL989924 |
Clitocella colorata | BJTC FM1892 | China | OL966945 | OL966956 | OL989915 | OL989919 | OL989925 |
Clitocella colorata | BJTC FM1952 | China | – | OL966958 | OL989916 | OL989920 | OL989926 |
Clitocella fallax | CBS 605.79 | – | AF357018 | – | – | – | – |
Clitocella fallax | CBS 129.63 | – | AF357017 | AF223166 | EF421018 | – | – |
Clitocella fallax | K(M): 116541 | Spain | – | – | KC816938 | KC816847 | KC816769 |
Clitocella fallax | O-F88953 | Norway | – | – | KC816936 | KC816845 | KC816767 |
Clitocella fallax | 25668OKM | USA | – | – | KC816937 | KC816846 | KC816768 |
Clitocella fallax | ME Noordeloos 1997173 | Italy | – | GQ289209 | GQ289275 | – | – |
Clitocella fallax | ME Noordeloos 200367 | Slovakia | – | GQ289210 | GQ289276 | – | – |
Clitocella mundula | 7161 TJB | USA | – | – | KC816952 | KC816862 | KC816782 |
‘Clitocella mundula’ | O-F19454 | Norway | – | – | KC816954 | KC816864 | KC816784 |
Clitocella mundula | O-F71544 | Norway | – | – | KC816950 | KC816860 | KC816780 |
‘Clitocella mundula’ | AFTOL-ID 521 | USA | – | – | KC816953 | KC816863 | KC816783 |
Clitocella mundula | 7115 TJB | USA | – | – | KC816951 | KC816861 | KC816781 |
Clitocella mundula | K(M): 164736 | UK | – | – | KC816949 | KC816859 | KC816779 |
‘Clitocella mundula’ | K(M): 49620 | UK | – | – | KC816948 | KC816858 | KC816778 |
Clitocella mundula | HMJAU 7274 | China | – | MN065724 | MN148161 | MN166272 | MN133781 |
Clitocella mundula | HMJAU 7275 | China | – | MN065723 | MN148160 | MN166271 | MN133780 |
Clitocella mundula | HMJAU 27014 | China | – | MN065722 | MN148159 | MN166270 | MN133779 |
Clitocella borealichinensis | BJTC FM1618 | China | OL966942 | OL966946 | OL989912 | – | OL989922 |
Clitocella borealichinensis | BJTC FM1781 | China | OL966943 | OL966957 | OL989913 | OL989917 | OL989923 |
Clitocella orientalis | HKAS 75548 | China | MN061333 | MN065727 | MN148164 | MN166275 | MN133784 |
Clitocella orientalis | HKAS 75664 | China | MN061332 | MN065726 | MN148163 | MN166274 | MN133783 |
Clitocella orientalis | HKAS 77899 | China | – | MN065725 | MN148162 | MN166273 | MN133782 |
Clitocella orientalis | HKAS 78876 | China | MN061334 | MN065729 | MN148166 | MN166277 | MN133786 |
Clitocella orientalis (Holotype) | HKAS 78763 | China | – | MN065728 | MN148165 | MN166276 | MN133785 |
Clitocella orientalis | BJTC FM1539 | China | – | OL966947 | OL989911 | OL989921 | – |
Clitocella popinalis | HBJU-550 | India | KU561066 | – | – | – | – |
Clitocella popinalis | CBS 481.50 | UK | FJ770397 | – | – | – | – |
Clitocella popinalis | KA12-1717 | Korea | KR673647 | – | – | – | – |
Clitocella popinalis | RA802-3b | USA | MK217434 | – | – | – | – |
Clitocella popinalis | Smith-2018 iNaturalist # 17340579 | USA | MK573922 | – | – | – | – |
Clitocella popinalis | K(M): 143166 | UK | – | – | KC816971 | KC816878 | KC816796 |
Clitocella popinalis | K(M): 167017 | UK | – | – | KC816972 | KC816879 | KC816797 |
Clitocella popinalis | O-F63376 | Norway | – | – | KC816974 | KC816880 | KC816799 |
Clitocella popinalis | 6378 TJB | Switzerland | – | – | KC816976 | KC816882 | KC816801 |
Clitocella popinalis | O-F105360 | Norway | – | – | KC816975 | KC816881 | KC816800 |
Clitocella popinalis | K(M): 146162 | UK | – | – | KC816970 | KC816877 | KC816795 |
‘Clitocella popinalis’ | MC2-TRENT | Italy | – | – | KC816973 | – | KC816798 |
‘Clitocella popinalis’ | ME Noordeloos 9867 | Austria | – | GQ289213 | GQ289280 | – | – |
Clitocella popinalis | TB6378 | USA | – | AF261285 | GU384654 | – | – |
Clitocella. Mundula | HMJAU 7275 | China | MN061331 | – | – | – | – |
Clitocella obscura | MK09051302 | Czech Republic | KX271753 | – | – | – | – |
Clitocella prunulus | G.v. Zanen F96065 | – | KC885965 | – | – | – | – |
Clitocella_termitophila | CORT014751 | Dominican Republic | – | – | MN893319 | – | – |
Clitopilus brunneiceps (Holotype) | HKAS 104510 | China | – | MN065684 | MN148123 | MN166234 | MN133737 |
Clitopilus yunnanensis (Holotype) | HKAS 104518 | China | – | MN065698 | MN148136 | MN166247 | MN133752 |
Clitopilus. Amarus | A. d. Haan 98031 | – | KC885963 | – | – | – | – |
Cltopilopsis albida (Holotype) | HKAS 104519 | China | – | MN065730 | MN148167 | MN166278 | MN133787 |
Lyophyllum decastes | Sundberg091007a | Japan | HM572548 | – | – | – | – |
Mycena pura | CBH371 | Denmark | KF913023 | – | – | – | – |
Rhodocybe mellea | CORT013885 | Dominican Republic | MN784992 | – | – | – | – |
Rhodocybe mellea | JBSD127402 | Dominican Republic | MN784993 | – | – | – | – |
Rhodocybe mellea | CORT014470 | Belize | MN784994 | – | – | – | – |
Rhodocybe mellea | NYBG815044 | Costa Rica | MN784995 | – | – | – | – |
The combined nrLSU-rpb2-tef1-atp6 dataset and ITS dataset were compiled to identify new species and to investigate their phylogenetic position in Clitocella. For the combined nrLSU-rpb2-tef1-atp6 dataset, Clitopilopsis albida S.P. Jian & Zhu L. Yang was chosen as outgroups for individual (nrLSU, rpb2, tef1, atp6) or combined analyses (
Maximum Likelihood (ML) was performed using RAxML 8.0.14 (
Trees were visualized with TreeView32 (
Twenty-eight sequences were newly generated from our six collections in this study. Two datasets, nrLSU-rpb2-tef1-atp6 combined dataset and ITS dataset were compiled to investigate the phylogenetic position of these Clitocella species. For the combined dataset, the phylogenetic trees based on individual loci (including nrLSU, rpb2, tef1, atp6) showed the almost same major clades (Suppl. material
Phylogeny derived from Maximum Likelihood analysis of the combined nrLSU-rpb2-tef1-atp6 dataset of Clitocella and related genera in the family Entolomataceae. Clitopilopsis albida was employed to root the tree as an outgroup. Numbers representing likelihood bootstrap support (BS≥ 70%, left) and significant Bayesian posterior probability (BPP≥ 0.95, right) are indicated above the nodes. New sequences are highlighted in bold.
The ITS dataset comprised 27 samples of 11 taxa and 662 characters. The topology of phylogenetic trees based on the ITS dataset generated from ML and BI analyses were almost identical and only the tree inferred from the ML analysis is shown (Fig.
Phylogeny derived from Maximum Likelihood analysis of the ITS sequences from Clitocella and related genera in the family Entolomataceae. Mycena pura was employed to root the tree as an outgroup. Numbers representing likelihood bootstrap support (BS≥ 70%, left) and significant Bayesian posterior probability (BPP≥ 0.95, right) are indicated above the nodes. New sequences are highlighted in bold.
borealichinensis, referring to north China as the place of origin.
China. Shanxi Province, Qinshui County, Lishan Mountain, 35°36.49'N, 112°11.7'E, alt. 1150m, 26 July 2021, on the ground in broad-leaved forest dominated by Quercus sp., N. Mao MNM340 (BJTC FM1781).
Clitocella borealichinensis is characterized by its clitocyboid basidiomata, globose to subglobose, occasionally broadly ellipsoid basidiospores, the absence of hymenial cystidia and clamp connection, and usually growing in broad-leaved forests. It is most similar to C. orientalis but differs from it by the slightly smaller basidiospores, non-gelatinized hyphae of pileipellis and stipitipellis with pale brown to brown intracellular or parietal pigment.
Basidiomata clitocyboid, small to medium-sized. Pileus 13–50 mm wide, low convex to plane convex when young, then slightly depressed at center; surface smooth, grayish white (#f2f2f2) to pale white (#ffffff), yellowish white (#ffcd9a); margin incurved, non-striate; context thin pale white, 1.0–1.2 mm thick. Lamellae decurrent, grayish white (#f2f2f2), pale yellow (#fff3e7), crowded, edges smooth, thin and fragile, lamellulae numerous and concolorous with lamellae. Stipe 20–32 × 2–8 mm, central to eccentric, occasionally lateral, cylindrical to subcylindrical, equal or sometimes slightly tapering at base, pale white (#ffffff), smooth or tomentose, usually with white rhizomorphs. Odor unrecorded. Taste not recorded. Chemical color reaction: not reacting with KOH 3% at pileus of dried specimens.
Basidiospores [60/2/2] (3.8–)4–5(–5.5) × 3.8–4.5 μm, Lm × Wm = 4.61 (± 0.42) × 4.06 (± 0.18), Q = 0.95–1.25 (Qav = 1.13 ± 0.10), hyaline, globose to subglobose, occasionally broadly ellipsoid in profile view, slightly angled in polar or face view with obscure minute pustules or bumps. Basidia 17–25 × 5–6(–7) μm, clavate, hyaline, four spored, rarely two spored; sterigmata 2–4 μm long. Lamellar trama more or less regular, composed of 3–8 μm wide hyaline hyphae, subhymenium consisting of filamentous hyphal segments. Lamellae edges fertile. Pleurocystidia and cheilocystidia absent. Pileipellis a cutis composed of more or less radially, loosely arranged, non-gelatinized, smooth, cylindrical hyphae, 2–6 μm wide and with pale brown to brown intracellular or parietal pigment; terminal hyphae subcylindric, narrowly clavate, occasionally irregular, 3–5 μm wide; subcutis made up of subparallel, compactly arranged, thin-walled, hyaline, smooth, cylindrical hyphae, 3–6 μm wide; pileal trama composed of interwoven, cylindrical hyphae, 2.5–10 μm wide. Stipitipellis a cutis composed of parallel, compactly arranged, thin-walled, non-gelatinized, hyaline hyphae, 2.5–6 μm wide. Stipititrama composed of interwoven, hyaline, cylindrical hyphae, 3–10 μm wide. Caulocystidia absent. Clamp connections absent.
Scattered or in groups on soil in broad-leaved (Quercus) forest, Shanxi province, China.
China. Shanxi province, Xia County, alt. 970m, 7 October 2020, N. Mao MNM172 (BJTC FM1618).
Clitocella borealichinensis is easily confused with C. orientalis, C. obscura (Pilát) Vizzini et al. and C. pallescens Silva-Filho & Cortez in morphology because they are all have white to grayish white pileus and decurrent lamellae. However, C. orientalis differs from C. borealichinensis by its viscid pileus and stipe when wet, gelatinized pileipellis and stipitipellis, and slightly larger basidiospores of (4–)4.5–6 × 4–5 μm (
Clitocella mundula and C. popinalis clustered with C. borealichinensis in our multilocus phylogeny (Fig.
colorata, referring to the colorful pileus.
China. Shanxi Province, Pu County, Wulushan Mountain, 36°33.2'N, 111°11.58'E, alt. 1740 m, 28 July 2021, on the ground in coniferous forest dominated by Pinus armandii Franch., N. Mao MNM292 (BJTC FM1891).
Clitocella colorata is characterized by its clitocyboid basidiomata, relatively colorful pileus (white to yellowish white, grayish white to grayish brown, pink white), globose or subglobose to broadly ellipsoid basidiospores, hyphae of pileipellis with pale yellow to yellowish brown intracellular or parietal pigment, the absence of hymenial cystidia and clamp connection. It is most similar to C. popinalis and C. mundula but differs from C. popinalis by its slightly smaller basidiospores, only appearing in the forest and genetic profile, and from C. mundula by its colorful pileus (white to yellowish white, grayish white to grayish brown, pink white).
Basidiomata clitocyboid, small to large. Pileus 20–62 mm wide, dry,convex to plano-convex, sometimes infundibuliform, with a shallow depression at the center; margin not striate, often enrolled or flat, sometimes slightly uplifted; surface white (#ffffff) to yellowish white (#ffffe7), grayish white (#f2f2f2) to grayish brown (#dba773), pink white (#fff3f5); context white (#ffffff) to grayish white (#f2f2f2), 1.0–1.5 mm thick. Lamellae decurrent, white (#ffffff) to yellowish white(#fff3e7), becoming yellowish brown (#e0b487) on drying, crowded, 1.0–2.0 mm deep, edges entire and concolorous, thin and fragile, lamellulae in 2–4 tiers of varying lengths. Stipe 22–42 × 4–10 mm, central, cylindrical, equal, pale white (#ffffff) to yellowish brown (#e0b487), smooth, usually with white rhizomorphs. Odor unrecorded. Taste not recorded. Chemical color reaction: pileal surface of dried samples negative with 3% KOH.
Basidiospores [100/5/2] (3.8–)4.5–5.5(–6.0) × (3.5–)4–4.8(–5.0) μm; Lm × Wm = 4.90 (± 0.44) × 4.29 (± 0.35), Q = 1.00–1.25 (Qav = 1.14 ± 0.09); hyaline, globose or subglobose to broadly ellipsoid in profile view, slightly angled in polar or face view with obscure minute pustules or bumps. Basidia 20–30 × (4.5–)5–6.5 μm, clavate, hyaline, with four spored, rarely two spored; sterigmata 2–3.5 μm long. Lamellar trama composed of subparallel, hyaline, cylindrical hyphae, 2.5–6 μm wide, subhymenium consisting of filamentous hyphal segments, 2–3.5 μm wide. Lamellae edges fertile. Pleurocystidia and cheilocystidia absent. Pileipellis a cutis composed of parallel, compactly arranged, non-gelatinized, smooth, cylindrical hyphae, 2–5 μm wide, with pale yellow to yellowish brown intracellular or parietal pigment; subcutis made up of interwoven, slightly loosely arranged, hyaline, smooth, cylindrical hyphae, 3–6.5 μm wide; pileal trama composed of parallel, compactly arranged, hyaline, cylindrical hyphae, 3–10 μm wide. Stipitipellis a cutis composed of parallel, compactly arranged, thin-walled, non-gelatinized, cylindrical hyphae, 2–5 μm wide, heavily or moderately encrusted with brown pigment. Stipititrama composed of parallel, compactly arranged, hyaline, cylindrical hyphae, 3–7 μm wide. Caulocystidia absent. Clamp connections absent.
Scattered or in groups on soil or rotten wood in coniferous (Pinus) or broad-leaved (Quercus) forest, Shanxi province, China.
China. Shanxi province, Pu County, Wulushan Mountains, alt. 1750m, 28 July 2021, N. Mao MNM293 (BJTC FM1892); Wenshui County, alt. 1760m, 30 July 2021, L. Fan CF1219 (BJTC FM1952); Xia County, alt. 931m, 6 October 2020, N. Mao MNM102 (BJTC FM1593); Xia County, alt. 931m, 6 October 2020, N. Mao MNM103 (BJTC FM1594).
Morphologically, Clitocella colorata is easily confused with C. mundula and C. popinalis. However, according to
Three species of Clitocella are confirmed from Shanxi Province, north China in this study. Of them, C. colorata is the most commonly encountered species, which distributes across the provincial area and grows in almost all kinds of forest. Clitocella orientalis and Clitocella borealichinensis are probably limited in southern Shanxi province, and they usually occur in the Quercus spp. forests.
ITS gene is rarely used in the species classification of Clitocella in previous studies because it contains many ambiguous sites. In the contrast, the partial sequences of three protein-coding genes (the atp6, rpb2 and tef1) are usually used to infer the phylogeny of Clitocella (
Interspecific variation and intraspecific variation of ITS in Clitocella species.
Species | Number (n) | Intraspecific variation (%) | Interspecific variation (%) |
---|---|---|---|
Clitocella colorata | 9 | < 1.6% | > 3.9% |
C. fallax | 3 | < 0.3% | > 11.8% |
C. mundula | 1 | – | > 6.0% |
C. borealichinensis | 2 | – | > 9.6% |
C. obscura | 1 | – | > 6.6% |
C. orientalis | 3 | < 0.9% | > 3.9% |
Interspecific variation and intraspecific variation of tef1 in Clitocella species.
Species | Number (n) | Intraspecific variation (%) | Interspecific variation (%) |
---|---|---|---|
Clitocella colorata | 4 | < 1.9% | > 4.1% |
C. fallax a | 1 | – | > 9.8% |
C. fallax b | 2 | < 0.1% | > 9.8% |
C. mundula | 6 | < 0.3% | > 7.5% |
‘C. mundula’c | 1 | – | > 4.7% |
C. borealichinensis | 1 | – | > 8.4% |
C. orientalis | 3 | < 0.1% | > 4.1% |
C. popinalis | 7 | – | > 4.7% |
Our molecular phylogenetic analysis (Fig.
Interspecific variation and intraspecific variation of rpb2 in Clitocella species.
Species | Number (n) | Intraspecific variation (%) | Interspecific variation (%) |
---|---|---|---|
Clitocella colorata | 4 | < 0.7% | > 1.7% |
C. fallax a | 1 | – | > 4.0% |
C. fallax b | 4 | < 0.1% | > 5.1% |
C. fallax c | 1 | – | > 4.0% |
C. mundula | 6 | < 2.1% | > 4.9% |
‘C. mundula’d | 1 | – | > 2.2% |
C. borealichinensis | 2 | – | > 5.5% |
C. orientalis | 6 | < 0.5% | > 1.7% |
C. popinalis | 9 | < 0.4% | > 2.2% |
C. termitophila | 1 | – | > 16.9% |
1 | Basidiomata clitocyboid | 2 |
– | Basidiomata pleurotoid |
C. termitophila
|
2 | Pileus surface gray, dark gray, pale yellow to yellowish brown, pigments present in pileipelli | 3 |
– | Pileus surface almost white to pastel gray, pigments absent in pileipellis | 8 |
3 | Basidiospores globose to subglobose | 4 |
– | Basidiospores ellipsoid | 7 |
4 | Pileus surface of dried samples with a positive KOH reaction | 5 |
– | Pileus surface of dried samples with a negative KOH reaction | 6 |
5 | Occurring in grassland systems |
C. popinalis
*( |
– | Occurring in forested systems |
C. mundula
*( |
6 | Pileus color with pink tinges | C. colorata * |
– | Pileus color without pink tinges | C. borealichinensis * |
7 | Pileus color with yellow tinges, basidiospores small, 5–8 × 3.5–5.5 μm |
C. himantiigena ( |
– | Pileus color without yellow tinges, basidiospores large, 7–9 × 5–6 μm |
C. ammophila ( |
8 | Basidiospores globose to subglobose or ovatae | 9 |
– | Basidiospores amygdaliform to ellipsoid | 11 |
9 | Basidia long, length > 40 μm |
C. nigrescens ( |
– | Basidia short, length < 28 μm | 10 |
10 | Pileus infundibuliform to plano-convex, basidiospores 4–5 × 3–4.5 μm |
C. pallescens ( |
– | Pileus convex to plane, basidiospores (4–)4.5–6 × 4–5 μm |
C. orientalis
* ( |
11 | Basidiospores small, 5–6.2 × 2.5–3.6 μm |
C. blancii ( |
– | Basidiospores large, 6.5–8 × 4–5 μm |
C. fallax
* ( |
We extend our appreciation to Dr. J.Z. Cao for collecting specimens and providing valuable suggestions. The study was supported by the National Natural Science Foundation of China (No. 31750001) and the Beijing Natural Science Foundation (No. 5172003).
Figure S1
Data type: JPG file
Explanation note: Phylogeny derived from Maximum Likelihood analysis of the nrLSU dataset of Clitocella and related genera in the family Entolomataceae. The bootstrap frequencies (> 70%) is shown on the supported branches. New species are highlighted in red.
Figure S2
Data type: JPG file
Explanation note: Phylogeny derived from Maximum Likelihood analysis of the rpb2 dataset of Clitocella and related genera in the family Entolomataceae. The bootstrap frequencies (> 70%) is shown on the supported branches. New species are highlighted in red.
Figure S3
Data type: JPG file
Explanation note: Phylogeny derived from Maximum Likelihood analysis of the tef1 dataset of Clitocella and related genera in the family Entolomataceae. The bootstrap frequencies (> 70%) is shown on the supported branches. New species are highlighted in red.
Figure S4
Data type: JPG file
Explanation note: Phylogeny derived from Maximum Likelihood analysis of the atp6 dataset of Clitocella and related genera in the family Entolomataceae. The bootstrap frequencies (> 70%) is shown on the supported branches. New species are highlighted in red.
ITS alignment
Data type: PHY file
Explanation note: The ITS dataset comprised 27 samples of 11 taxa and 662 characters.