Research Article |
Corresponding author: Aimin Pang ( ppam@tom.com ) Corresponding author: Heng Gui ( guiheng@mail.kib.ac.cn ) Academic editor: Rungtiwa Phookamsak
© 2021 Guang-Cong Ren, Dhanushka N. Wanasinghe, Jutamart Monkai, Peter E. Mortimer, Kevin D. Hyde, Jian-Chu Xu, Aimin Pang, Heng Gui.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Ren G-C, Wanasinghe DN, Monkai J, Mortimer PE, Hyde KD, Xu J-C, Pang A, Gui H (2021) Novel saprobic Hermatomyces species (Hermatomycetaceae, Pleosporales) from China (Yunnan Province) and Thailand. MycoKeys 82: 57-79. https://doi.org/10.3897/mycokeys.82.67973
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During our survey of the diversity of woody litter fungi in China and Thailand, three Hermatomyces species were collected from dead woody twigs of Dipterocarpus sp. (Dipterocarpaceae) and Ehretia acuminata (Boraginaceae). Both morphology and multigene analyses revealed two taxa as new species (Hermatomyces turbinatus and H. jinghaensis) and the remaining collections as new records of H. sphaericus. Hermatomyces turbinatus is characterized by 1) dimorphic conidia, having circular to oval lenticular conidia and 2) turbinate conidia consisting of two columns with two septa composed of 2–3 cells in each column. Hermatomyces jinghaensis is characterized by dimorphic conidia, having circular to oval lenticular conidia and clavate or subcylindrical to cylindrical conidia and consisting of one or two columns with 6–8 cells in each column. Phylogenetic analyses of combined LSU, ITS, tub2, tef1-α and rpb2 sequence data supports the placement of these new taxa within Hermatomycetaceae with high statistical support.
2 new species, hyphomycetes, phylogeny, taxonomy, woody litter fungi
Over the past few decades, the number of studies using a molecular-based approach to study microfungal diversity in the greater Mekong subregion (GMS) has increased rapidly, especially on freshwater and woody litter fungi from China (Yunnan Province) and Thailand (
Hermatomyces was introduced by
Based on morphological comparisons and phylogenetic affinities,
Our investigation led to the discovery of three Hermatomyces species, including two novel species, on dead woody-based substrates. Morphological illustrations and multi-gene phylogenetic analyses with ML, MP and BI of combined LSU, ITS, tub2, tef1-α and rpb2 sequence data are used to confirm the phylogenetic placement of the novel species within Hermatomyces.
Woody litter samples were collected from China (Yunnan Province) during the dry season (December 2019) and Thailand (Tak Province) during the wet season (August 2019). Samples were brought to the laboratory using plastic Ziploc bags. Fungal specimens were then examined using a stereomicroscope (Olympus SZ61, China). Pure cultures were obtained via single spore isolation on potato dextrose agar (PDA) following the methods described in
DNA extraction, amplification, sequencing, sequence alignment and phylogenetic analyses follow the methods of
GenBank accession numbers of sequences used for the phylogenetic analyses.
Organism | Strain number | GenBank accession numbers | Reference | ||||
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LSU | ITS | tub2 | tef1-α | rpb2 | |||
Anteaglonium globosum | ANM 925.2T | GQ221879 | NA | NA | GQ221925 | NA |
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A. parvulum |
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KU922915 | NA | NA | KU922921 | NA |
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Hermatomyces amphisporus |
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LR812664 | LR812664 | NA | NA | NA |
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H. amphisporus |
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NA | LR812663 | LR812674 | LR812658 | LR812669 |
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H. amphisporus |
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NA | LR812665 | LR812675 | LR812659 | LR812670 |
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H. amphisporus |
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LR812662 | LR812662 | LR812673 | LR812657 | LR812668 |
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H. amphisporus |
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LR812666 | LR812666 | LR812676 | LR812660 | LR812671 |
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H. amphisporus |
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LR812667 | LR812667 | LR812677 | LR812661 | LR812672 |
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H. bauhiniae |
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MK443378 | MK443382 | NA | MK443384 | MK443386 |
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H. biconisporus | KUMCC 17-0183T | MH260296 | MH275063 | NA | MH412771 | MH412755 |
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H. bifurcatus | CCF 5899 | LS398262 | LS398262 | LS398441 | LS398416 | LS398343 |
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H. bifurcatus | CCF 5900T | LS398263 | LS398263 | LS398442 | LS398417 | LS398344 |
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H. clematidis |
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MT214556 | MT310603 | NA | MT394735 | MT394684 |
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H. constrictus | CCF 5904T | LS398264 | LS398264 | LS398443 | LS398418 | LS398345 |
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H. indicus |
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KU764692 | KU144920 | NA | KU872754 | KU712488 |
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H. indicus |
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KU764693 | KU144921 | NA | KU872755 | KU712489 |
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H. indicus |
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KU764694 | KU144922 | NA | KU872756 | KU712490 |
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H. iriomotensis | KH 361T | LC194367 | LC194483 | NA | LC194394 | LC194449 |
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H. jinghaensis | HKAS 112167T | MW989519 | MW989495 | NA | MZ042642 | NA | This study |
H. krabiensis |
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KX525742 | KX525750 | NA | KX525758 | KX525754 |
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H. krabiensis (H. chiangmaiensis) |
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KY559394 | NA | NA | NA | NA |
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H. megasporus | CCF 5897 | NA | LS398265 | LS398444 | LS398419 | LS398346 |
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H. megasporus | CCF 5898T | LS398266 | LS398266 | LS398445 | LS398420 | NA |
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H. nabanheensis | KUMCC 16-0149T | KY766059 | KY766058 | NA | KY766061 | NA |
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H. pandanicola |
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KX525743 | KX525751 | NA | KX525759 | KX525755 |
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H. reticulatus | CCF 5893 | LS398267 | LS398267 | LS398446 | LS398421 | LS398347 |
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H. reticulatus (H. subiculosus) |
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KX259523 | KX259521 | NA | KX259527 | KX259529 |
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H. sphaericoides | CCF 5896 | NA | LS398271 | LS398448 | LS398425 | LS398351 |
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H. sphaericoides | CCF 5908T | LS398273 | LS398273 | LS398450 | LS398427 | LS398352 |
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H. sphaericoides | CCF 5907 | NA | LS398272 | LS398449 | LS398426 | NA |
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H. sphaericoides | CCF 5895 | LS398270 | LS398270 | LS398447 | LS398424 | LS398350 |
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H. sphaericus |
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LS398281 | LS398281 | LS398454 | LS398431 | LS398356 |
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H. sphaericus |
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NA | LS398283 | LS398455 | LS398432 | LS398357 |
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H. sphaericus | PRM 946201 | NA | LS398284 | LS398456 | LS398433 | LS398358 |
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H. sphaericus |
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NA | LS398275 | NA | NA | NA |
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H. sphaericus |
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NA | LS398286 | NA | NA | NA |
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H. sphaericus |
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NA | LS398278 | LS398453 | LS398430 | LS398355 |
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H. sphaericus |
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NA | LS398277 | LS398452 | LS398429 | LS398354 |
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H. sphaericus |
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NA | LS398279 | NA | NA | NA |
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H. sphaericus |
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NA | LS398280 | NA | NA | NA |
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H. sphaericus |
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NA | LS398285 | NA | NA | NA |
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H. sphaericus | KZP 462 | NA | LS398287 | LS398457 | LS398434 | LS398359 |
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H. sphaericus |
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NA | LS398276 | NA | NA | NA |
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H. sphaericus (H. chromolaenae) |
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KY559393 | NA | NA | NA | NA |
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H. sphaericus (H. saikhuensis) |
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KX525740 | KX525748 | NA | KX525756 | KX525752 |
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H. sphaericus (H. saikhuensis) |
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KX525741 | KX525749 | NA | KX525757 | KX525753 |
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H. sphaericus (H. tectonae) |
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KU764695 | KU144917 | NA | KU872757 | KU712486 |
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H. sphaericus (H. tectonae) |
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KU764696 | KU144918 | NA | KU872758 | NA |
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H. sphaericus (H. tectonae) |
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KU764697 | KU144919 | NA | NA | KU712487 |
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H. sphaericus |
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MW989516 | MW989492 | MZ042643 | MZ042639 | MZ042636 | This study |
H. sphaericus | KUMCC 20-0231 | MW989517 | MW989493 | MZ042644 | MZ042640 | MZ042637 | This study |
H. trangensis |
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KY790600 | KY790598 | NA | KY790606 | KY790604 |
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H. trangensis |
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KY790601 | KY790599 | NA | KY790607 | KY790605 |
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H. tucumanensis | CCF 5912 | LS398288 | LS398288 | LS398458 | LS398435 | LS398360 |
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H. tucumanensis | CCF 5913 | LS398289 | LS398289 | LS398459 | LS398436 | LS398361 |
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H. tucumanensis | CCF 5915 | LS398290 | LS398290 | LS398460 | LS398437 | LS398362 |
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H. turbinatus |
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MW989518 | MW989494 | MZ042645 | MZ042641 | MZ042638 | This study |
H. verrucosus | CCF 5903T | LS398292 | LS398292 | LS398462 | LS398439 | LS398364 |
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H. verrucosus | CCF 5892 | LS398291 | LS398291 | LS398461 | LS398438 | LS398363 |
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Representative species used in the phylogenetic analyses were selected based on previous publications (
ML was carried out in CIPRES Science Gateway v.3.3 (http://www.phylo.org/portal2/;
Bayesian analysis was executed in MrBayes v.3.2.2 (
Phylogenetic trees were visualized in FigTree v1.4.0 (http://tree.bio.ed.ac.uk/software/figtree/;
Ex-type strains are indicated with superscript “T”, and newly generated sequence is shown in bold. NA represents sequences that are unavailable in GenBank. Abbreviations:
ANM A.N. Miller;
CCF Culture Collection of Fungi, Charles University, Prague, Czech Republic;
HKAS The herbarium of Cryptogams Kunming Institute of Botany Academia Sinica;
KH K. Hirayama;
KUMCC Culture Collection of Kunming Institute of Botany, Kunming, China;
KZP O. Koukol;
PRM Herbarium of the National Museum, Prague, Czech Republic.
T1 Type of Hermatomyces thailandicus;
T2 Type of H. chiangmaiensis;
T3 Type of H. subiculosus;
T4 Type of H. chromolaenae;
T5 Type of H. saikhuensis;
T6 Type of H. tectonae.
The phylogenetic analysis was conducted using 57 strains in Hermatomycetaceae, and two outgroup taxa Anteaglonium globosum (ANM 925.2) and A. parvulum (
In the phylogenetic tree obtained from ML, MP and BI analysis (Fig.
Phylogenetic RAxML tree based on analysis of a combined LSU, ITS, tub2, tef1-α and rpb2 and dataset. Bootstrap support values for ML and MP equal to or higher than 75% and Bayesian PP equal to or greater than 0.95 are shown at nodes. Hyphens (--) represent support values less than 75% / 0.95 BYPP. The ex-type strains are in bold and the new isolate in this study is in blue bold. The tree is rooted with Anteaglonium globosum (ANM 925.2) and A. parvulum (
Referring to the turbinate shape of the conidia.
HKAS 112724.
Saprobic on woody litter of Dipterocarpus sp. (Dipterocarpaceae) Sexual morph Undetermined. Asexual morph Colonies on natural substrate forming sporodochial conidiomata, superficial, scattered, small groups, circular or oval, sterile mycelial outer zone enclosing a black-brown velvety margin, sparse, black sporulating center, shiny, glistening, circular or oval, conidia readily liberated when agitated. Mycelium superficial, branched, septate, hyaline to pale brown, 2–3 μm wide. Conidiophores 6–8 × 2–3 μm, micronematous, straight or flexuous, smooth, short, pale brown. Conidiogenous cells 3–5 × 2–3 μm, monoblastic, integrated, terminal, determinate, often arising directly on the superficial mycelium, subsphaerical, ovoid or ampulliform, hyaline to pale brown, smooth finely verruculose. Conidia dimorphic, solitary, smooth-walled. Lenticular conidia 24–30 × 17–21 μm (x = 27 × 20 μm, n = 20), 12–15 μm thick, thick-walled, circular to oval in front view, smooth, solitary, muriform, central cells dark brown to black, peripheral cells hyaline to pale brown, forming a weakly ring, sometimes slightly constricted at septa, obovoid or oblong in lateral view, arranged in 2 rows, a row of composed of 4–6 cells, end cells pale brown to hyaline, middle cells dark brown. Turbinate conidia turbinate, pyriform, 27–36 μm in length, 19–28 µm wide in broadest part of lower cells, (x = 32 × 23 μm, n = 20), asymmetrical with the upper cells smaller than lower cells, thick-walled, smooth, septate, constricted distinct at septa, consisting of two columns with two septa composed of 2–3 rectangular to globose cells in each column, usually upper part of terminal cells dark brown, becoming hyaline towards the lower side, two cells hyaline in the lower cells swollen with oil globules.
Hermatomyces turbinatus (HKAS 112724, holotype) a, b sporodochia on natural substrate c vertical section of sporodochium d conidiophores and conidiogenous cells e–h turbinate conidia i turbinate and mature lenticular conidia j–m mature lenticular conidia n germinated conidium o, p culture characters on PDA. Scale bars: 30 μm (c); 20 μm (d–n); 30 mm (o, p).
Dipterocarpus sp. (Thailand).
Colonies on PDA, reaching 30–40 mm diam., after 3 weeks at 25–30 °C, circular, convex with papillate and radially furrowed at the center, rough, labate, crenate edge, fluffy, dense, gray black, in reverse darkens at the center, pale brown to gray at edge.
Thailand, Tak Province. Ban Na Sam Ngao District, on woody litter of Dipterocarpus sp. (Dipterocarpaceae), 22 August 2019, G. C. Ren, TSY04 (HKAS 112724, holotype), ex-type living culture,
Hermatomyces turbinatus is introduced as a new species based on its distinct morphology, which is supported by phylogenetic analyses. In the phylogenetic analyses, H. turbinatus is distinct from extant species in this genus and formed a sister clade to H. nabanheensis with strong support (94% ML, 91% MP, 1.00 PP; Fig.
Species | Lenticular conidia size (μm) | Cylindrical / turbinate conidia feature | Host | Country | Reference | ||
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Shape | Length × width (μm) | Number of columns (cells) | |||||
Hermatomyces amphisporus | 27–36(–38) × 18–29(–31) | Cylindrical, pyriform or turbinate | 30‒38 × 20‒26 | 2(–4) (6–12 cells) | Cyathea sp., Sabal minor | Mexico, USA |
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H. bauhiniae | 25–36 × 15–20 | Cylindrical | 20–28 × 8–11 | 1 (2–3-septate) | Bauhinia variegata | Thailand |
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H. biconisporus | 28–34 × 15–25 | Cylindrical | 32–39 × 14.5–26 | 1–2 (3–4 cells) | Pandanus sp. | China |
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H. bifurcatus | (24–)30–36.5(–41) × (18–)21.5–26(–28) | Cylindrical | Apex: 7–16 × 7–12 Basal: 9–14 × 13–18.5 | 2 (2–3 cells) | Unknown | Panama |
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H. chromolaenae | 9.2–10.4 × 10.2–11.5 | NA | NA | NA | Chromolaena odorata | Thailand |
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H. clematidis | 30–45 × 24–31 | Cylindrical | 29–35 × 12–14 | 1–2 (5–6 cells) | Clematis sikkimensis | Thailand |
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H. constrictus | (22–)25.5–29.5(–32) × 19–23.5(–27.5) | Cylindrical | Lower cells: (20–)24–30.5(–37) × 12–17 Upper cells: (16–)20–26(–30) × 8–14 | 1 (2 cells) | Bauhinia cumanensis | Panama |
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H. dimorphus | 35‒55 × 15‒20 | Cylindrical | 15‒40 × 10‒15 | 4 (7 cells) | Unknown | India |
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H. indicus | 18‒30 × 11.5‒19 | Turbinate | 22.4‒35.4 × 11.4‒21.6 | 2 (6–7 cells) | Phoenix rupicola | India |
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H. iriomotensis | 30–36 × 20–27 | Cylindrical | 20.5–33 × 7–12.5 | 1–2 (3–7 cells) | Unknown | Japan |
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H. jinghaensis | 30–40 × 25–30 | Clavate, subcylindrical | 33–43 × 11–13 | 1–2 (6–8 cells) | Unknown | China | This study |
H. krabiensis | 24.3–32.5 × 12.1–21.3 | Cylindrical | 20.4–26.4 × 8.6–19.7 | 1–2 (2–3 cells) | Pandanus odorifer | Thailand |
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H. megasporus | (45–)49–56(–59) × (31–)37–46 | Cylindrical | (37–)49.5–60.5(67–) × 18–28(–32) | 2 ((5–)6–7(–10) cells) | Unknown | Panama |
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H. nabanheensis | 20.2–25.1 × 16.6–20.7 | Cylindrical | 15.3–26.8 × 12.1–18.2 | 1–2 (2–3 cells) | Pandanus sp. | China |
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H. pandanicola | 12–15.7 × 20–30.1 | Cylindrical | 13.2–20.6 × 8.9–11.9 | 2 (2 cells) | Pandanus odorifer | Thailand |
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H. reticulatus | 3–40(–45) × 25–34(–41) | NA | NA | NA | Unknown | Thailand, Panama |
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H. saikhuensis | 14.2–21.4 × 11.2–19.3 | NA | NA | NA | Pandanus odorifer | Thailand |
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H. sphaericoides | (20.5–)24.5–28(–31) × (20–)23–26(–29) | NA | NA | NA | Unknown | Panama |
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H. sphaericus ( |
(21–)24–29(–32.5) × (18–)21–27(–31.5) | NA | NA | NA | Various host plants | Tropical or subtropical |
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H. sphaericus | 27–29 × 26–28 | NA | NA | NA | Dipterocarpus sp., Ehretia acuminata | China, Thailand | This study |
H. tectonae | (23–)26–29(–33) × (19–)23–25(–28) | Cylindrical | (27–)31–32(–35) ×(21–)23 | 2 (6 cells) | Tectona grandis | Thailand |
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H. trangensis | 27.5‒35 × 25‒32.5 | NA | NA | NA | Arenga pinnata | Thailand |
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H. truncates | (26–)31.5–36.5(–37) × 22–27(–30) | Cylindrical | Lower cells: 14–22.5(–28) × 8.5–14.5 | 1 (2–3 cells) | Averrhoa carambola | Ghana, Panama | Koukol et al. (2019) |
Upper cells: 12–18(–30) × (6–)8–12.5 | |||||||
H. tucumanensis | (22–)27–35 × 18–25 | Obclavate or subcylindrical | (21–)23–26(–28.5) × 7–14 | 2 (3–6 cells) | Unknown | Panama |
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H. turbinatus | 24–30 × 17–21 | Turbinate | 27–36 × 19–28 | 2 (2–3 cells) | Dipterocarpus sp. | Thailand | This study |
H. uniseriatus | 27–36 × 15.5–24 | Cylindrical | 19–34 × 10–12.5 | 1 (3–4 cells) | Smilax campestris | Argentina |
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H. verrucosus | 23–30(–39) × 21–29.5 | NA | NA | NA | Unknown | Panama |
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The species epithet “jinghaensis” refers to the location where the species was collected.
HKAS 112167.
Saprobic on unidentified woody litter. Sexual morph Undetermined. Asexual morph Colonies on natural substrate forming sporodochial conidiomata, superficial, scattered, small groups, circular, sterile mycelial outer zone enclosing a black velvety margin, dense, thick, black sporulating center, shiny, glistening, circular or oval, conidia readily liberated when agitated. Mycelium superficial, branched, septate, hyaline to pale brown, 2–3 μm wide. Conidiophores 30–45 × 2–3 μm, mononematous, cylindrical, straight or flexuous, smooth, pale brown. Conidiogenous cells 4–6 × 2–3 μm, monoblastic, integrated, terminal, determinate, often arising directly on the superficial mycelium, cylindrical, ampulliform, hyaline to pale brown, smooth finely verruculose. Conidia dimorphic solitary, smooth-walled. Lenticular conidia 30–40 × 25–30 μm (x = 37 × 28 μm, n = 20), 21–25 μm thick, thick-walled, circular to oval in front view, smooth, solitary, muriform, central cells brown to dark brown, peripheral cells hyaline to subhyaline, forming a wide and distinct ring, sometimes slightly constricted at septa, obovoid or oblong in lateral view, central cells brown to dark brown, peripheral cells pale brown to brown. Cylindrical conidia 33–43 μm in length, 11–13 µm wide in broadest part of lower cells (x = 39 × 12 μm, n = 20), clavate or subcylindrical, straight or flexuous, septate, constricted distinct at the septa, with large guttules, consisting of one or two columns, each column with 6–8 cells, apical cell rectangular to globose, smooth, hyaline, smooth, basal cells acute, rectangular to cylindrical, pale brown.
Unidentified woody litter (China)
China, Yunnan Province, Xishuangbanna Dai Autonomous Prefecture, Jinghong, Jingha (21°78.06'N, 101°05.61'E), on unidentified woody litter, 19 December 2019, D.N. Wanasinghe, DW57 (HKAS 112167, holotype), no living culture.
Hermatomyces jinghaensis is introduced as a new species based on its distinct morphology and the phylogenetic results of a combined LSU, ITS, tub2, tef1-α and rpb2 dataset. Hermatomyces jinghaensis nested with H. clematidis and H. trangensis in a strongly supported monophyletic group (99% ML, 100% MP, 1.00 PP; Fig.
Saprobic on woody litter of Dipterocarpus sp. (Dipterocarpaceae) and Ehretia acuminata (Boraginaceae). Sexual morph Undetermined. Asexual morph Colonies on natural substrate forming sporodochial conidiomata, superficial, circular or irregular, scattered or crowded, consisting of a velvety, dense, annular, gray brown, sterile mycelial outer zone and a black, glistening, abundantly sporulating granulose center, with conidia readily liberated when agitated. Mycelium 2–2.5 μm wide, superficial, composed of a tightly network of branched, septate, smooth or finely verruculose, hyaline or pale brown hyphae. Conidiophores 10–13 × 2–4 μm (x = 12 × 3 μm, n = 10) micronematous, cylindrical or forked, smooth, hyaline or pale brown, often corresponding to conidiogenous cells. Conidiogenous cells 5–8 × 3–5 μm (x = 7 × 4 μm, n = 20), monoblastic, integrated, terminal, cylindrical, hyaline to pale brown, smooth or finely verruculose. Conidia of one type, 27–29 × 26–28 μm (x = 28 × 27 μm, n = 30) μm, 19–24 μm thick, solitary, lenticular, globose, subglobose in front view, muriform, smooth, central cells brown, dark brown, outer ring of peripheral cells narrow, pale brown to brown, often constricted at septa, disk-shaped in lateral view, consisting of two rows, each row with 4–6 cells, hyaline to light brown at lower and upper cells, middle cells brown to black brown.
Hermatomyces sphaericus (HKAS 112725) a, b colonies on the natural substrate c mycelia d–g young conidia h–k mature conidia (h–j surface view k thickness view) l germinated conidium m, n culture characters on PDA. Scale bars: 1000 μm (a); 200 μm (b); 20 μm (c–i, l); 30 μm (j, k); 3 cm (m, n).
Tropical and subtropical regions of Central and South America, Africa, Asia, Oceania and North America. The species were found as saprobes on Acanthaceae, Apocynaceae, Arecaceae, Asteraceae, Dipterocarpaceae, Euphorbiaceae, Fabaceae, Lamiaceae, Leguminosae, Mimosaceae, Nyctaginaceae, Oxalidaceae, Pandanaceae, Pinaceae, Rhamnaceae, and Sterculiaceae (
Colonies on PDA, reaching 35–40 mm diam., after 3 weeks at 25–30 °C, with circular, umbonate, fluffy, velvety, entire edge, a circular raised band, gray white, in reverse dark gray, black toward the center.
Thailand, Tak Province, Tha Song Yang District, on woody litter of Dipterocarpus sp. (Dipterocarpaceae), 22 August 2019, G. C. Ren, T903 (HKAS 112725), living culture,
The characters of our new strain of Hermatomyces sphaericus (KUMCC 20-0231,
This study introduces two new species of woody-based litter fungi; Hermatomyces jinghaensis from Yunnan, China and Hermatomyces turbinatus on Dipterocarpus sp. from Thailand. We also report for the first time two new records of H. sphaericus on Dipterocarpus sp. and Ehretia acuminata in China and Thailand.
Hermatomyces (Hermatomycetaceae) is different from other similar genera in its sporodochial conidiomata and in having one to two (lenticular and cylindrical conidia) unusual conidial types (
Hermatomyces sphaericus was introduced by
Species delineation in Hermatomyces, especially in the H. sphaericus clade, is subject to much controversy due to species inconsistency in morphological and phylogenetic status.
Hermatomyces had long been treated as “incertae sedis” within Ascomycota (
In this study, we combined two non-translated loci (LSU, ITS) and three protein-coding regions (tub2, tef1-α and rpb2) to carry out phylogenetic analysis for Hermatomyces species in order to validate phylogenetic placement of the taxa within Hermatomyces. In our phylogenetic analyses, H. tectonae, H. chromolaenae, H. biconisporus, H. pandanicola and H. saikhuensis grouped together with strains of H. sphaericus (
This work was supported by Open Research Fund Program of Science and Technology on Aerospace Chemical Power Laboratory (STACPL320181B04). We thank the support from the National Natural Science Foundation of China (NSFC21975066, NSFC21875061). We also would like to thank the Thailand Research Fund for the grant entitled Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion (No. RDG6130001). Dhanushka Wanasinghe thanks CAS President’s International Fellowship Initiative (PIFI) for funding his postdoctoral research (number 2021FYB0005), the Postdoctoral Fund from Human Resources and Social Security Bureau of Yunnan Province and the National Science Foundation of China and Chinese Academy of Sciences (grant no. 41761144055) for financial support.