Research Article |
Corresponding author: Zhan-Bo Liu ( zhanboliu@bjfu.edu.cn ) Academic editor: Maria-Alice Neves
© 2021 Ping Du, Tian-Xu Cao, Ying-Da Wu, Meng Zhou, Zhan-Bo Liu.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Du P, Cao T-X, Wu Y-D, Zhou M, Liu Z-B (2021) Two new species of Hymenochaetaceae on Dracaena cambodiana from tropical China. MycoKeys 80: 1-17. https://doi.org/10.3897/mycokeys.80.63997
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Two new wood-rotting fungi in the family Hymenochaetaceae, Fulvifomes dracaenicola sp. nov. and Hymenochaete dracaenicola sp. nov., are described and illustrated from tropical China based on morphological characteristics and molecular data. It is worth to mention that both of them grow on Dracaena cambodiana which is a kind of angiosperm tree distributed in tropical regions. F. dracaenicola is characterised by perennial, pileate, triquetrous basidioma with yellowish brown fresh pores which becoming honey yellow with silk sheening upon drying, a dimitic hyphal system in trama and monomitic in context, and subglobose basidiospores measuring 4.8–5 × 4–4.1 μm. H. dracaenicola is characterised by annual, resupinate basidioma with a clay buff hymenophore, a dimitic hyphal system, absence of tomentum and cortex, presence of subulate setae, absence of cystidia, presence of cystidioles and simple hyphidia, and oblong ellipsoid basidiospores measuring 5.2–5.8 × 2.5–2.8 µm. The phylogenetic analyses based on ITS + nLSU rDNA sequences confirm the placement of two new species respectively in Fulvifomes and Hymenochaete. Phylogenetically closely related species to the two new species are discussed.
Phylogenetic analysis, taxonomy, wood-rotting fungi
Fulvifomes Murrill (Hymenochaetaceae, Hymenochaetales) was erected in 1914 and typified by F. robiniae (Murrill) Murrill (
Hymenochaete Lév. (Hymenochaetaceae, Hymenochaetales) was erected in 1846 and typified by H. rubiginosa (Dicks.) Lév. (
During investigations on the diversity of wood-rotting fungi from China, five unknown specimens were collected from Hainan Province, and their morphology corresponds to the concepts of Fulvifomes and Hymenochaete. To confirm their affinity, phylogenetic analyses based on the ITS and nLSU rDNA sequences were carried out. Both morphological characteristics and molecular evidence demonstrated these five specimens represent two new species of Hymenochaetaceae, which we describe in the present paper.
Macro-morphological descriptions were based on field notes and dry herbarium specimens. Microscopic measurements and drawings were made from slide preparations of dried tissues stained with Cotton Blue and Melzer’s reagent following
A CTAB rapid plant genome extraction kit (Aidlab Biotechnologies Co., Ltd., Beijing, China) was used to extract total genomic DNA from dried specimens following the manufacturer’s instructions with some modifications (
Phylogenetic trees were constructed using ITS and nLSU rDNA sequences, and phylogenetic analyses were computed with maximum likelihood (ML), maximum parsimony (MP), and Bayesian inference (BI) methods. Sequences of Fulvifomes were adopted mainly from ITS + nLSU tree topologies established by
A list of species, specimens and GenBank accession numbers of sequences used in the phylogenetic analysis of Fulvifomes.
Taxa | Voucher | ITS | LSU |
---|---|---|---|
Fomitiporella caryophylli | CBS 448.76 | AY558611 | AY059021 |
Fulvifomes centroamericanus | JV 0611/III | KX960763 | KX960764 |
F. centroamericanus | JV 0611/8P | KX960757 | — |
F. dracaenicola | Dai 22093 | MW559799 | MW559804 |
F. dracaenicola | Dai 22097 | MW559800 | MW559805 |
F. fastuosus | LWZ 20140731-13 | KR905674 | KR905668 |
F. fastuosus | LWZ 20140718-29 | KR905673 | — |
F. fastuosus | Dai 18292 | MH390411 | MH390381 |
F. grenadensis | JV 1212/2J | KX960756 | — |
F. grenadensis | JV 1607/66 | KX960758 | — |
F. hainanensis | Dai 11573 | KC879263 | JX866779 |
F. halophilus | XG 4 | JX104705 | JX104752 |
F. halophilus | JV 1502/4 | MH390427 | MH390392 |
F. imbricatus | LWZ 20140728-16 | KR905677 | KR905670 |
F. imbricatus | LWZ 20140729-25 | KR905678 | — |
F. imbricatus | LWZ 20140729-26 | KR905679 | KR905671 |
F. indicus | Yuan 5932 | KC879261 | JX866777 |
F. indicus | O 25034 | KC879262 | KC879259 |
F. krugiodendri | JV 0904/1 | KX960762 | KX960765 |
F. krugiodendri | JV 0312/24.10J | KX960760 | KX960766 |
F. krugiodendri | JV 1008/21 | KX960761 | KX960767 |
F. merrillii | — | JX484013 | — |
F. nilgheriensis | URM 3028 | MH390431 | MH390384 |
F. nilgheriensis | PPT152 | MH048095 | MH048085 |
F. rimosus | M 2392655 | MH628255 | MH628017 |
F. robiniae | CBS 211.36 | AY558646 | AF411825 |
F. robiniae | — | EF088656 | — |
F. siamensis | XG 2 | JX104709 | JX104756 |
F. siamensis | Dai 18309 | MH390434 | MH390389 |
F. sp. | PM 950703-1 | EU035311 | — |
F. squamosus | CS385 | MF479268 | MF479265 |
F. squamosus | CS444 | MF479269 | MF479264 |
F. submerrillii | Dai 17911 | MH390405 | MH390371 |
F. submerrillii | Dai 17917 | MH390406 | MH390372 |
F. thailandicus | LWZ 20140731-1 | KR905672 | KR905665 |
F. xylocarpicola | MU 8 | JX104676 | JX104723 |
Inocutis rheades | — | AF237731 | — |
Inonotus hispidus | CBS 388.61 | AY558602 | — |
I. lloydii | Dai 10809 | MH390428 | MH390378 |
I. lloydii | Dai 9642 | MH390429 | MH390379 |
I. lloydii | Dai 11978 | MH390430 | MH390380 |
I. porrectus | CBS 296.56 | AY558603 | AY059051 |
I. rigidus | Dai 17496 | MH390432 | MH390398 |
I. rigidus | Dai 17507 | MH390433 | MH390399 |
Phellinotus neoaridus | URM 80362 | KM211294 | KM211286 |
P. piptadeniae | URM 80766 | KM211293 | KM211285 |
Phellinus laevigatus | CBS 122.40 | MH856059 | MH867554 |
P. populicola | CBS 638.75 | MH860960 | MH872729 |
Phylloporia crataegi | Dai 18133 | MH151191 | MH165865 |
P. ribis | CBS 579.50 | MH856765 | MN240818 |
A list of species, specimens and GenBank accession numbers of sequences used in the phylogenetic analysis of Hymenochaete.
Taxa | Voucher | ITS | LSU |
---|---|---|---|
Hydnoporia tabacina | He 390 | JQ279610 | JQ279625 |
Hymenochaete acerosa | He 338 | JQ279543 | JQ279657 |
H. adusta | He 207 | JQ279523 | KU975497 |
H. angustispora | Dai 17045 | MF370592 | MF370598 |
H. angustispora | Dai 17049 | MF370593 | MF370599 |
H. anomala | He 592 | JQ279566 | JQ279650 |
H. asetosa | Dai 10756 | JQ279559 | JQ279642 |
H. attenuata | He 28 | JQ279526 | JQ279633 |
H. bambusicola | He 4116 | KY425674 | KY425681 |
H. berteroi | He 1488 | KU975459 | KU975498 |
H. biformisetosa | He 1445 | KF908247 | KU975499 |
H. boddingii | MEH 66068 | MN030343 | MN030345 |
H. boddingii | MEH 69996 | MN030341 | MN030347 |
H. boddingii | MEH 66150 | MN030344 | MN030344 |
H. borbonica | CBS 731.86 | MH862026 | MH873716 |
H. cana | He 1305 | KF438169 | KF438172 |
H. cinnamomea | He 755 | JQ279548 | JQ279658 |
H. colliculosa | Dai 16427 | MF370595 | MF370602 |
H. colliculosa | Dai 16428 | MF370596 | MF370603 |
H. colliculosa | Dai 16429 | MF370597 | MF370604 |
H. conchata | MEH 70144 | MF373838 | — |
H. contiformis | He 1166 | KU975461 | KU975501 |
H. cruenta | He 766 | JQ279595 | JQ279681 |
H. cyclolamellata | Cui 7393 | JQ279513 | JQ279629 |
H. damicornis | URM 84261 | KC348466 | — |
H. damicornis | URM 84263 | KC348467 | — |
H. denticulata | He 1271 | KF438171 | KF438174 |
H. dracaenicola | Dai 22090 | MW559797 | MW559802 |
H. dracaenicola | Dai 22096 | MW559798 | MW559803 |
H. duportii | AFTOL ID666 | DQ404386 | AY635770 |
H. epichlora | He 525 | JQ279549 | JQ279659 |
H. floridea | He 536 | JQ279597 | JQ279683 |
H. fuliginosa | He 1188 | KU975465 | KU975506 |
H. fulva | He 640 | JQ279565 | JQ279648 |
H. globispora | He 911 | — | KU975508 |
H. huangshanensis | He 432 | JQ279533 | JQ279671 |
H. hydnoides | He 245 | JQ279590 | JQ279680 |
H. innexa | He 555 | JQ279584 | JQ279674 |
H. legeri | He 960 | KU975469 | KU975511 |
H. longispora | He 217 | JQ279537 | KU975514 |
H. luteobadia | He 8 | JQ279569 | KU975515 |
H. macrochloae | ARAN-Fungi 7079 | MF990738 | MF990743 |
H. megaspora | He 302 | JQ279553 | JQ279660 |
H. minor | He 933 | JQ279555 | JQ279654 |
H. minuscula | He 253 | JQ279546 | KU975516 |
H. murina | He 569 | JQ716406 | JQ716412 |
H. muroiana | He 405 | JQ279542 | KU975517 |
H. nanospora | He 475 | JQ279531 | JQ279672 |
H. ochromarginata | He 47 | JQ279579 | JQ279666 |
H. odontoides | Dai 11635 | JQ279563 | JQ279647 |
H. orientalis | He 4601 | KY425677 | KY425685 |
H. parmastoi | He 867 | JQ780063 | KU975518 |
H. paucisetigera | Cui 7845 | JQ279560 | JQ279644 |
H. quercicola | He 373 | KU975474 | KU975521 |
H. rhabarbarina | He 280 | JQ279574 | KY425688 |
H. rheicolor | Cui 8317 | JQ279529 | — |
H. rhododendricola | He 389 | JQ279577 | JQ279653 |
H. rubiginosa | He 1049 | JQ716407 | JQ279667 |
H. rufomarginata | He 1489 | KU975477 | KU975524 |
H. separabilis | He 460 | JQ279572 | JQ279655 |
H. setipora | Cui 6301 | JQ279515 | JQ279639 |
H. sharmae | CAL 1535 | KY929017 | KY929018 |
H. sharmae | 66088 | MK588753 | MK588836 |
H. spathulata | He 685 | JQ279591 | KU975529 |
H. sphaericola | He 303 | JQ279599 | JQ279684 |
H. sphaerospora | He 715 | JQ279594 | KU975531 |
H. subferruginea | Cui 8122 | JQ279521 | — |
H. subferruginea | He 1598 | KU975481 | — |
H. tasmanica | He 449 | JQ279582 | JQ279663 |
H. tongbiguanensis | He 1552 | KF908248 | KU975532 |
H. tenuis | He 779 | JQ279538 | JQ279641 |
H. tropica | He 574 | JQ279587 | JQ279675 |
H. ulmicola | He 864 | JQ780065 | KU975534 |
H. unicolor | He 468a | JQ279551 | JQ279662 |
H. verruculosa | Dai 17047 | — | MF370600 |
H. verruculosa | Dai 17052 | MF370594 | MF370601 |
H. villosa | He 537 | JQ279528 | JQ279634 |
H. xerantica | Cui 9209 | JQ279519 | JQ279635 |
H. yunnanensis | He 1447 | KU975486 | KU975538 |
Maximum parsimony analysis was applied to the ITS + nLSU dataset sequences. Approaches to phylogenetic analysis followed
MrModeltest 2.3 (
Branches that received bootstrap support for maximum likelihood (BS), maximum parsimony (BP), and Bayesian posterior probabilities (BPP) greater than 70% (BS), 50% (BP) and 0.95 (BPP) were considered as significantly supported, respectively. FigTree v1.4.2 (
The combined ITS + nLSU dataset included sequences from 50 specimens representing 31 species (Table
The phylogeny (Fig.
Phylogeny of Fulvifomes and related species by MP analysis based on combined ITS and nLSU rDNA sequences. Branches are labelled with maximum likelihood bootstrap > 70%, parsimony bootstrap proportions > 50%, and Bayesian posterior probabilities > 0.95, respectively. New species is in bold.
The combined ITS + nLSU dataset included sequences from 79 specimens representing 69 species (Table
The phylogeny (Fig.
Phylogeny of Hymenochaete and related species by ML analysis based on combined ITS and nLSU rDNA sequences. Branches are labelled with maximum likelihood bootstrap > 70%, parsimony bootstrap proportions > 50%, and Bayesian posterior probabilities > 0.95, respectively. New species is in bold.
Fulvifomes dracaenicola is characterised by perennial, pileate, triquetrous basidioma with yellowish brown fresh pores which becoming honey yellow with silk sheening upon drying, a dimitic hyphal system in trama and monomitic in context, subglobose basidiospores measuring 4.8–5 × 4–4.1 μm.
China. Hainan Province, Sanya, Daxiaodongtian Park, N18.299, E109.172, on living tree of Dracaena cambodiana, 15.XI.2020, Dai 22097 (
Dracaenicola (Lat.): referring to the species growing on Dracaena cambodiana.
Basidioma perennial, pileate, without odor or taste and woody hard when fresh, light in weight when dry. Pilei triquetrous, projecting up to 2.5 cm, 2.3 cm wide and 2.6 cm thick at base. Pileal surface yellowish brown to grayish brown when fresh, vinaceous brown when dry, encrusted, glabrous, zonate, uncracked, margin olivaceous brown. Pore surface yellowish brown when fresh, honey yellow with silk sheening when dry; sterile margin indistinct; pores circular, 5–7 per mm; dissepiments thin, entire. Context cinnamon buff to fawn, corky, often darker near the pileus surface, up to 1.4 cm thick, with a distinct crust (black line) near pileus surface at the basal area, partly with additional crust (black line) within context or above tubes. Tubes cinnamon buff to cinnamon, woody hard, up to 1.2 cm thick, tube layers distinctly stratified, individual tube layer up to 0.5 cm long.
Hyphal system dimitic in trama, monomitic in context; generative hyphae simple septate; tissues darkening but otherwise unchanged in KOH.
Generative hyphae apricot-orange to brownish-orange, thick-walled with a wide lumen, simple septate, unbranched, regularly arranged, 4.5–6 µm in diam.
Generative hyphae hyaline, thick-walled, simple septate, occasionally branched, 2–2.5 mm in diam; skeletal hyphae apricot-orange to brownish-orange, thick-walled to subsolid, unbranched, loosely interwoven, 3.5–4 mm in diam. Setae or setal hyphae absent; hymenium collapsed in the studied material, basidia and basidioles not seen.
Basidiospores subglobose with an apiculus, yellowish brown, thick-walled, smooth, IKI–, CB–, occasionally collapsed when mature, 4.8–5(–5.5) × 4–4.1 μm, L = 5.02 μm, W = 4.04 μm, Q = 1.22–1.25 (n = 90/3).
China. Hainan Province, Sanya, Daxiaodongtian Park, N18.299, E109.172, on rotten wood of living Dracaena cambodiana, 15.XI.2020, Dai 22093 (
Hymenochaete dracaenicola is characterised by annual, resupinate basidioma with a clay buff hymenophore, a dimitic hyphal system, absence of tomentum and cortex, subulate setae present in hyphal layer, absence of cystidia, presence of cystidioles and simple hyphidia, and oblong ellipsoid basidiospores measuring 5.2–5.8 × 2.5–2.8 µm.
China. Hainan Province, Sanya, Daxiaodongtian Park, N18.299, E109.172, on dead tree of Dracaena cambodiana, 15.XI.2020, Dai 22090 (
Dracaenicola (Lat.): referring to the species s growing on Dracaena cambodiana.
Basidioma annual, resupinate, adnate, not separable from substrate, hard corky, up to 7.5 cm long, 2 cm wide, and less than 0.1 mm thick at center. Hymenophore surface smooth or locally verruculose, clay buff, with some scattered crevices; margin cinnamon buff, up to 0.4 mm.
Hyphal system dimitic; generative hyphae infrequent, simple septate; skeletal hyphae dominant; tissues darkening but otherwise unchanged in KOH.
Tomentum and cortex absent; hyphal layer present. Generative hyphae infrequent, hyaline, thick-walled, simple septate, often branched, 1–2 µm in diam. Skeletal hyphae cinnamon to orange brown, thick-walled to subsolid, rarely branched, interwoven, 1.5–2.5 µm in diam.
Hyphae similar to those in hyphal layer. Setal layer present, thickening with age, with one to several rows of overlapping setae. Setae numerous, subulate with blunt to acute tips, orange brown to reddish brown, smooth, occasionally with a hyphal sheath, distinctly thick-walled, 30–57 × 6–10 µm, embedded or projecting up to 35 µm beyond the hymenium. Cystidia absent; cystidioles present, fusoid, hyaline, thin-walled, basally swollen, with a sharp or often hyphoid neck, 10–17 × 2.5–4 μm; Simple hyphidia present, scattered, thick-walled, 15–36 × 2–3.5 µm. Basidia subclavate to subcylindrical, with walls thickening toward the base, with four sterigmata and a basal simple septum, 17–23(–25) × 3.5–5 µm; basidioles similar to basidia but smaller.
Basidiospores oblong ellipsoid with an apiculus, hyaline, thin-walled, smooth, IKI–, CB–, occasionally bearing a guttule, (5–)5.2–5.8(–6.1) × 2.5–2.8 µm, L = 5.6 µm, W = 2.68 µm, Q = 2.03–2.15 (n = 60/2).
China. China. Hainan Province, Sanya, Daxiaodongtian Park, N18.299, E109.172, on fallen branch of Dracaena cambodiana, 15.XI.2020, Dai 22096 (
Fulvifomes dracaenicola and Hymenochaete dracaenicola were found in tropical regions of China. It is interesting that both species growing on Dracaena cambodiana.
Morphologically, Fulvifomes dracaenicola (5–7 per mm) shares similar pores with F. kawakamii (M.J. Larsen, Lombard & Hodges) T. Wagner & M. Fisch. (5–7 per mm,
Two specimens of Fulvifomes dracaenicola form a lineage with strong support (100% BP, 100% BS, 1.00 BPP, Fig.
Morphologically, to avoid redescribing the existed species, we review the monograph by
The research is supported by the National Natural Science Foundation of China (Project No. 31900019).