First record of the rare genus Typhrasa (Psathyrellaceae, Agaricales) from China with description of two new species

Sheng-Nan Wang; Ya-Ping Hu; Jun-Liang Chen; Liang-Liang Qi; Hui Zeng; Hui Ding; Guang-Hua Huo; Lin-Ping Zhang; Fu-Sheng Chen; Jun-Qing Yan

doi:10.3897/mycokeys.79.63700

Research Article

First record of the rare genus Typhrasa (Psathyrellaceae, Agaricales) from China with description of two new species

Sheng-Nan Wang^‡, Ya-Ping Hu^§, Jun-Liang Chen^|, Liang-Liang Qi^¶, Hui Zeng^#, Hui Ding^§, Guang-Hua Huo^‡, Lin-Ping Zhang^‡, Fu-Sheng Chen^‡, Jun-Qing Yan^‡

‡ Jiangxi Agricultural University, Nanchang, China

§ Institute of Environmental Sciences, Nanjing, China

| Science and Technology Research Center of Edible Fungi, Qingyuan, China

¶ Microbiology Research Institute, Guangxi Academy of Agriculture Sciences, Nanning, China

# Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, China

Corresponding author: Fu-Sheng Chen ( chenfush@hotmail.com )

Corresponding author: Jun-Qing Yan ( yanjunqing1990@126.com )

Academic editor: R. Henrik Nilsson

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Wang S-N, Hu Y-P, Chen J-L, Qi L-L, Zeng H, Ding H, Huo G-H, Zhang L-P, Chen F-S, Yan J-Q (2021) First record of the rare genus Typhrasa (Psathyrellaceae, Agaricales) from China with description of two new species. MycoKeys 79: 119-128. https://doi.org/10.3897/mycokeys.79.63700

Abstract

Typhrasa is a rare genus that comprises two species and that has previously been reported only from Europe and North America. The present study expands the geographical scope of the genus by describing two new species – T. polycystis and T. rugocephala – from subtropical China. The new species are supported by morphological characteristics and phylogenetic analyses (ITS, LSU and tef-1α). The new species have very similar morphological characteristics and are 98% similar in their ITS region. However, T. rugocephala has two types of long gills at the same time, rarely fusiform pleurocystidia with rostrum. Detailed descriptions, colour photos, illustrations and a key to related species are presented in this paper.

Keywords

Basidiomycota, macromycetes, morphology, phylogenetic analysis, taxonomy

Introduction

The genus Typhrasa Örstadius & E. Larss. was established in 2015. It is characterised by a hygrophanous cap, crowded gills with white edge, small-to-medium-sized spores, large hymenial cystidia with intracellular oily drops or globules and a hymeniderm or paraderm pileipellis (Örstadius et al. 2015). The genus includes two species, T. gossypina (Bull.) Örstadius & E. Larss. and T. nanispora Örstadius, Hauskn. & E. Larss., the former being previously reported occasionally from some countries in Europe, North America and Asia within the genus Psathyrella (Fr.) Quél (Smith 1972; Kits van Waveren 1985; Knudsen and Vesterholt 2012; Örstadius et al. 2015). In addition, Psathyrella delineata (Peck) A.H. Sm., P. canadensis A.H. Sm. and P. subtenacipes A.H. Sm. are also reported to have oily drops in their cystidia (Smith 1972; Kits van Waveren 1985) and seems to be a candidate for Typhrasa. However, P. delineata and P. canadensis were combined into T. gossypina, based on the morphology (Örstadius et al. 2015). During investigations in subtropical China during 2018–2020, Typhrasa was recorded for the first time in China with two unrecorded species, which were frequently collected. Based on morphological characters and phylogenetic analyses, they are described as new species in this paper.

Materials and methods

Morphological studies

Macromorphological characters and habitat details were noted from fresh, young to mature basidiomata (over five basidiomata for each species) in the field. The location of the collection point is marked on the map (Suppl. material 1: Fig. S1). Colour codes are from the Methuen Handbook of Colour (Kornerup and Wanscher 1978). Micromorphological characters were observed with a light microscope (Olympus BX53). Sections from dry specimens were observed in water, 5% aqueous potassium hydroxide (KOH) solution, 10% aqueous ammonia (NH₃·H₂O) solution and Melzer’s Reagent, separately. More than fifty basidiospores, cystidia and basidia in 5% aqueous KOH solution were measured under the microscope. Basidiospore measurements were recorded in front and profile view. The measurements and Q values are given as (a)b–c(d), in which “a” is the lowest value, “b–c” covers a minimum of 90% of the values and “d” is the highest value. “Q” represents the ratio of length to width of a spore (Bas 1969; Ge et al. 2017; Na and Bau 2019). Specimens are deposited in the Herbarium of Fungi, Jiangxi Agricultural University (HFJAU) and Herbarium of Mycology, Jilin Agricultural University (HMJAU).

DNA extraction and sequencing

DNA was extracted from dried specimens with the NuClean Plant Genomic DNA kit (CWBIO, China). Three regions (ITS, LSU and Tef-1α) were generated for the study, which were amplified with primers ITS1/ITS4 (White et al. 1990), LR0R/LR7 (Hopple and Vilgalys 1999) and EF983F/EF2218R (Örstadius et al. 2015), respectively. PCR was performed using a touchdown programme: 5 min at 95 °C; 1 min at 95 °C; 30 s at 65 °C (add -1 °C per cycle); 1 min at 72 °C; 15 cycles; 1 min at 95 °C; 30 s at 50 °C; 1 min at 72 °C; 20 cycles; and 10 min at 72 °C (Yan and Bau 2018). The DNA sequencing was done by Qing Ke Biotechnology Co. Ltd. (Wuhan City, China).

Data analyses

The ITS, LSU and Tef-1α datasets were assembled following Örstadius et al. (2015) and BLAST in GenBank. Sequences from a total of 24 taxa were analysed using five data partitions (ITS, LSU, Tef 1^st, Tef 2^nd and Tef 3^rd). The details are presented in Table 1. Sequences were aligned separately in MAFFT v.7 (Katoh and Standley 2013). The best-fit models of nucleotide evolution for ITS, LSU, Tef 1^st, Tef 2^nd and Tef 3^rd datasets (GTR+G, GTR+I, SYM, SYM and GTR+G, respectively) were obtained in MrModeltest v.2.3 (Nylander et al. 2008). Phylogenetic analysis was conducted using Bayesian Inference (BI) in MrBayes v.3.2.6 (Ronquist et al. 2012). Gaps were treated as missing data following Örstadius et al. (2015). Four Monte Carlo Markov Chains (MCMC) were run for five million generations, sampling every 100^th generation. The first 25% of trees were discarded as burn-in (Ronquist et al. 2012). The sequence alignment is deposited in TreeBASE (http://purl.org/phylo/treebase/phylows/study/TB2:S27860).

Table 1.

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CSV

XLSX

Sequences used in this study. Newly generated sequences are given in bold. Type material is indicated in the column Voucher.

Taxa	Voucher	Locality	ITS	LSU	tef-1α
Cystoagaricus hirtosquamulosus	Ramsholm800927	Finland	KC992945	KC992945	–
C. olivaceogriseus	WK 8/15/63-5 (MICH) Type	USA	KC992948	KC992948	–
C. sylvestris	LÖ191-92	Sweden	KC992949	KC992949	–
C. squarrosiceps	Laessoe44835	Ecuador	KC992950	–	–
C. strobilomyces	E. Nagasawa 9740		AY176347	AY176348	–
Kauffmania larga	LAS97-054	Sweden	DQ389695	DQ389695	–
K. larga	LÖ223-90	Sweden	DQ389694	DQ389694	KJ732824
Psathyrella delineata	CCB171	USA	KY744151
P. delineata	TMW02	USA	MF686534
P. delineata	MGW1406	USA	KY777378
Typhrasa gossypina	180524-H08	Korea	MN082538	–	–
T. gossypina	BRNM:705622	Austria	AM712293	–	–
T. gossypina	BRNM:705609	Czech	AM712292	–	–
T. gossypina	WU:25069	Austria	AM712294	–	–
T. gossypina	Schumacher024	Germany	KC992946	KC992946	KJ732825
T. nanispora	Barta980706 Type	Austria	KC992947	KC992947	–
T. polycystis	HFJAU1454 Type	China:Jiangxi	MW466538	MW466544	MW475280
T. polycystis	HFJAU1520	China:Fujian	MW466539	MW466545	MW475281
T. polycystis	HFJAU1349	China:Jiangxi	MW466540	–	–
T. rugocephala	HFJAU1467 Type	China:Zhejiang	MW466541	MW466546	MW475282
T. rugocephala	HFJAU1455	China:Zhejiang	MW466542	MW466547	MW475283
T. rugocephala	HFJAU1476	China:Zhejiang	MW466543	MW466548	–
Outgroup
Psathyrella oboensis	DED 8234 Type	SãoTomé	NR148107	–	–
P. pertinax	LO259-91 Neotype	Sweden	DQ389701	DQ389701	KJ732809

Results

Based on the BLAST results of the full length of the ITS region, two new species were found sharing less than 98.0% similarity with the known species of Typhrasa, respectively: 97% with T. gossypina and 92% with T. nanispora. The Bayesian analysis (Figure 1) comprised material from three major genera, all of which are identified as monophyletic with very strong support (BPP = 1), viz. Typhrasa, Cystoagaricus Singer and Kauffmania Örstadius & E. Larss., in agreement with the study published by Örstadius et al. (2015). Our collections of the two new Typhrasa species formed a joint, strongly supported clade (BPP = 1) and both new species received strong support as monophyletic (BPP ≥ 0.97). The collections of T. gossypina (Bull.) Örstadius & E. Larss. clustered together and appeared as a sister to the group consisting of the two new species.

Figure 1.

Phylogram generated by Bayesian Inference (BI) analysis, based on sequences of a concatenated dataset from three nuclear markers (ITS, LSU and tef-1α) rooted with Psathyrella spp. Bayesian posterior probabilities ≥ 0.95 are shown. ● indicates the newly-described species.

Taxonomy

Typhrasa polycystis J.Q Yan & S.N. Wang, sp. nov.

MycoBank No: 838482

Fig. 2

Diagnosis

Differs from Typhrasa gossypina by its smaller spores (7.1–8.2 × 4.3–5.1 μm).

Holotype

China. Jiulianshan National Nature Reserve, Jiangxi Province, 25 June 2020, Jun-Qing Yan, HFJAU1454.

Etymology

Referring to the characteristics of the pleurocystidia.

Description

Pileus 20–35 mm, extending hemispherically to expanded, plane with or without umbo, surface with slightly ridge-like folds or smooth, hygrophanous, brown (7D6–7C6), pale brown (6B6–6C6) at the margin. Veil distinct, fibrous or fluffy, white (7C1), markedly appendiculate at margin, falling off easily. Context thin and fragile, hygrophanous at pileus, about 3.0 mm at the centre. Gills 4.0–5.0 mm broad, moderately close, pale cinnamon (6C6–6D5) with a white (6C1) edge, adnexed. Stipe 30–45 mm long, 5.0–8.0 mm thick, white (6C1), hollow, pulverulent at apex, with fibrils and fluffy from pellicular veil remnants below, falling off easily.

Spores (6.9)7.1–8.2 × (4.2)4.3–5.1(5.2) μm, Q = (1.4)1.5–1.8(1.9), ellipsoid to oblong-ellipsoid, profile flattened on one side, 4.1–5.0(5.2) μm broad, smooth, reddish-brown (8C5–8C6) in water, yellow-brown (7D6–7D7) in 5% KOH or 10% NH₃·H₂O, becoming darker (7E4–7F4) in 5% KOH, germ pore small and indistinct, 1–2 guttulate, inamyloid. Basidia 21–24 × 7.5–8.5 μm, 4-spored, clavate, hyaline. Pleurocystidia 55–81(87) × 11–17(18) μm, variously shaped, often fusiform and lageniform, with rostrum; rarely fusiform with subacute to acute apex or cystiform with rostrum, with one or two large internal oily drops, oily drops colourless and distinct or indistinct in 5% KOH, glassy-yellow (5B6–5B7) and very distinct in Melzer’s Reagent. Cheilocystidia (24)30–54(58) × (9)10–15(16) μm, similar to pleurocystidia, abundant, rarely mixed with pyriform or clavate cells. Trama of gills consisting of parallel hyphae. Pileipellis a 2–3 cells deep layer of subglobose or pyriform cells which are 24–36 μm wide. Veil composed of hyphae 6.5–14.5 μm-broad, thin-walled and fawn (5A2-A3) hyphae in 5% KOH. Clamps present in trama of gills, hyphae of stipe and at the base of the basidia and cystidia.

Ecology and distribution

Saprotrophic, solitary to slightly caespitose on rotten hard wood or humus in mixed forests.

Other specimens examined

China. Jiulianshan National Nature Reserve, Jiangxi Province, 28 May 2018, Guang-Hua Huo, Lin-Ping Zhang, HFJAU1349. Wuyishan National Nature Reserve, Fujian Province, 27.748888°N, 117.7625°E, 761 alt., 12 June 2020, Liangliang Qi, Yupeng Ge, HFJAU1520, HMJAU58461.

Figure 2.

Basidiomata and microscopic features of Typhrasa polycystis a–c basidiomata d basidiospores e basidia f pleurocystidia g cheilocystidia. Scale bars: 20 mm (a–c); 10 μm (d–g).

Typhrasa rugocephala J.Q Yan & S.N. Wang, sp. nov.

MycoBank No: 838483

Fig. 3

Diagnosis

Differs from Typhrasa polycystis by having two types of long gills and rarely rostrum can be found in fusiform pleurocystidia.

Holotype

China. Baishanzu National Nature Reserve, Zhejiang Province, 27.734233°N, 119.186943°E, 1184 m alt. 28 June 2020, Sheng-Nan Wang, HFJAU1467.

Etymology

Referring to the surface of the pileus.

Description

Pileus 35–55 mm, spreading hemispherically to oblate with a slight umbo, surface with distinct ridge-like folds, hygrophanous, reddish-brown (8E5–8F6), pale brown (6D7–6C7) at the margin, drying tawny (7D6–7E6), striate, sometimes faintly, at margin. Veil distinct, fibrous or fluffy, white (7C1), markedly appendiculate at margin, falling off easily. Context thin and fragile, hygrophanous, about 2.5 mm at the centre. Gills 5.0–7.0 mm broad, moderately close; when young, dirty white (7B1), becoming cinnamon (7C6–7D5) with a white edge (7C1); two types of long gills arranged at intervals: A: adnate to slightly decurrent, B: emarginate- adnexed. Stipe 40–60 mm long, 5.0–10 mm thick, white (7C1), hollow, pulverulent at apex, with fibrils and fluffy from pellicular veil remnants below, falling off easily.

Spores (6.5)6.8–7.9(8.3) × 4.5–5.2(5.4) μm, Q = (1.3)1.4–1.7, ellipsoid to oblong-ellipsoid, profile flattened on one side, 4.1–5.0(5.2) μm broad, smooth, reddish-brown (8C6–8C7) in water, yellow-brown (7D6–7D7) in 5% KOH or 10% NH₃·H₂O, becoming darker (7E4–7E5) in 5% KOH, germ pore small and indistinct, 1–2 guttulate, inamyloid. Basidia 18–23 × 7.0–8.0 μm, 4-spored, clavate, hyaline. Pleurocystidia 42–68 × 13–17 μm, thin-walled, fusiform, apex obtuse to subacute, rarely cystiform with a short rostrum, with one or two large internal oily drops, oily drops colourless and distinct or indistinct in 5% KOH, glassy-yellow (5B6–5B7) and very distinct in Melzer’s Reagent. Cheilocystidia scanty, 33–48 × 10–15 μm, similar to pleurocystidia, few and scattered, mix with pyriform or clavate, 21–39 × 11–13 μm-sized cells. Trama of gills consisting of parallel hyphae. Pileipellis a 2–3 cells deep layer of subglobose or pyriform cells which are 18–32 μm wide. Veil composed of 5.4–8.4 μm-broad hyphae, thin-walled and fawn (5A2-A3) hyphae in 5% KOH. Clamps rare, but observed in trama of gills, hyphae of stipe and at the base of the basidia and cystidia.

Figure 3.

Basidiomata and microscopic features of Typhrasa rugocephala a–d basidiomata e basidiospores f basidia g pleurocystidia h cheilocystidia. Scale bars: 20 mm (a–d); 10 μm (e–h).

Ecology and distribution

Saprotrophic, solitary or gregarious on soil or humus in broad-leaved forests.

Other specimens examined

China. Baishanzu National Nature Reserve, Zhejiang Province, 24 June 2020, Ya-Ping Hu, HFJAU1476; 28 June 2020, Sheng-Nan Wang, HFJAU1455, HMJAU58462.

Discussion

Typhrasa was established by Örstadius et al. (2015), based on the main characters of having rostrate, hymenial cystidia with oily drops. Only T. gossypina and T. nanispora Örstadius, Hauskn. & E. Larss. were reported in that study. T. gossypina, as the type species of the genus, was, therefore, separated from Psathyrella (Fr.) Quél. This species can be separated from the two new species through its longer spores up to 9.0 μm long, 5.0–6.0 μm broad in front view and pleurocystidia often with a long rostrum (Kits van Waveren 1985; Örstadius et al. 2015). T. nanispora has smaller spores, 5.0–6.0 × 3.0–4.0 μm and can be thereby easily be distinguished (Örstadius et al. 2015). T. rugocephala is very easily confused with T. polycystis, but the former has two types of long gills arranged at intervals, scanty cheilocystidia and rarely rostrum can be found in fusiform pleurocystidia. In addition, P. subtenacipes are also reported to have oily drops in their cystidia (Smith 1972) and seems to be a candidate for Typhrasa, but study of type material is needed to settle this question. Morphologically, the spores of P. subtenacipes are up to 7.8–9.5 × 5.0–5.6 μm, significantly larger than the two new species (Smith et al. 1950; Smith 1972). A key to these related species is presented below:

Key to related species

1	Spores up to 9.5 μm long, 5.0–5.6 μm broad	*P. subtenacipes*
–	Not as above	2
2	Spores less than 6.0 μm long	*T. nanispora*
–	Spores over 6.0 μm long	3
3	Spores 7.0–9.0 μm long, 5.0–6.0 μm broad in front view	*T. gossypina*
–	Spores smaller, less than 8.0 μm long and 5.0 μm broad in front view	4
4	Long gills have two types concurrently: adnate to slightly decurrent and emarginate-adnexed, fusiform, apex obtuse to subacute, rarely cystiform with a short rostrum, cheilocystidia scanty	*T. rugocephala*
–	Gills adnexed, pleurocystidia variously-shaped, often fusiform and lageniform with rostrum	*T. polycystis*

Acknowledgements

This work is supported by the National Natural Science Foundation of China (31960008), Jiangxi Provincial Natural Science Foundation (20202BABL213041). The project was supported by the biodiversity investigation, observation and assessment programme (2019–2023) of the Ministry of Ecology and Environment of China (2110404); Central Public-Interest Scientific Institution Basal Research Fund (GYZX200203). Sincere thanks to the anonymous reviewers of the manuscript.

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Supplementary material

Supplementary material 1

Figure S1. Collection site of Typhrasa rugocephala and T. polycystis

Sheng-Nan Wang, Ya-Ping Hu, Jun-Liang Chen, Liang-Liang Qi, Hui Zeng, Hui Ding, Guang-Hua Huo, Lin-Ping Zhang, Fu-Sheng Chen, Jun-Qing Yan

Data type: image

This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.

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