Research Article
Print
Research Article
Two new species and a new record of yellow Cantharellus from tropical Quercus forests in eastern Mexico with the proposal of a new name for the replacement of Craterellus confluens
expand article infoLeticia Montoya, Mariana Herrera, Victor M. Bandala, Antero Ramos
‡ Instituto de Ecología A.C., Xalapa, Mexico
Open Access

Abstract

Two new species of yellow Cantharellus and a new record of Cantharellus tabernensis associated with tropical species of Quercus are presented, based on the taxonomic study of fresh specimens and in a phylogenetic analysis of transcription elongation factor 1-alpha (tef-1α) and the large subunit of the ribosome (nLSU) sequences. One of the new species proposed here, corresponds to a choice edible mushroom, which, in our molecular phylogeny, resulted in it being related to the group of species around C. lateritius and sister with Craterellus confluens type specimen. This latter is here formally transferred to Cantharellus and consequently a new name, Cantharellus furcatus, is proposed to replace the homonym Cantharellus confluens (Schwein.) Schwein. 1834 a later synonym of Byssomerulius corium. Detailed macroscopic and microscopic descriptions accompanied with illustrations and a taxonomic discussion are presented for each species.

Keywords

American Cantharellus, Craterellus, ectomycorrhizal mushrooms, Neotropical Cantharellus or chanterelles, oak, wild edible mushrooms

Introduction

In the American continent, especially from USA, new species of Cantharellus had been proposed, several of them look-alikes of the commonly cited C. cibarius Fr., C. cinnabarinus Fr. and C. lateritius (Berk.) Singer (Arora and Dunham 2008; Buyck et al. 2010, 2011, 2016a, b; Buyck and Hofstetter 2011; Foltz et al. 2013; Leacock et al. 2016; Thorn et al. 2017). Further explorations in tropical America are achieving also the discovery of undescribed species of the genus (e.g. Wartchow et al. 2012; Henkel et al. 2014; Nascimento et al. 2014; Buyck et al. 2016b; Herrera et al. 2018) as occurred also with Craterellus (Wilson et al. 2012).

Species delimitation in Cantharellus is often said to be hard to address, especially because of the overlap of phenotypic variation, including scarce microscopic morpho-anatomic taxonomically informative features. In such a sense, Buyck et al. (2014) explicitly defined that basidiomes of Cantharellus species “…under the microscope …exhibit a discouraging monotony…”. Studying Cantharellus specimens from Mexico, we have noted that the difficulty in revising early records is exacerbated by frequent incomplete data accompanying herbaria specimens. For or instance, there is poor or no information on features like hymenophore and color variations of basidiomes along their development or even by weathering effects. It is of primary importance then, to be able to count on accurate observations of specimens in fresh that lead to the characterization of their phenotypes and establish robust concepts for pertinent taxonomic conclusions. It is important even to count on data on the spatial/temporal distribution, and associated tree species.

In Cantharellus violaceovinosus (Herrera et al. 2018), for example, it was possible to document wide macromorphological and color information through a register of samples collected over more than five years, even in weekly explorations along three years sampling. Such a record allowed us to recognize its phenology between July-October in pure stands of Quercus oleoides, and found it less frequent in association with Q. glaucescens and Q. sapotifolia. In fact, such a record together with molecular information facilitated the distinction of C. violaceovinosus from other phenotypically similar species. Olariaga et al. (2015) informed about the identity of some taxa previously described solely based on colored or unpigmented variants, i.e., while in C. amethysteus (Quel.) Sacc., C. cibarius Fr., C. ferruginascens P.D. Orton, C. pallens Pilát and C. romagnesianus Eyssart. & Buyck, white specimens may occasionally occur, in C. cibarius and C. pallens orange forms can be found. Among other conclusions, these authors demonstrated that white forms of C. cibarius already described as varieties (var. inodorus Velen, f. pallidus R. Schulz) corresponded molecularly indeed to a single taxon, and C. gallaecicus (Blanco-Dios) Olariaga, lacking yellow-orange tones, is in fact the same as the orange-yellow to ochre-yellow C. romagnesianus (Olariaga et al. 2017).

Yellow chantherelles, such as Cantharellus cibarius Fr., C. lateritius (Berk.) Singer, C. odoratus (Schwein.) Fr. and Craterellus confluens Berk. & M.A. Curtis have been reported from different regions of Mexico (Berkeley 1867; Guzmán and Sampieri 1984; Guzmán 1985; Guevara et al. 2004; Pérez-Moreno et al. 2008; Garibay-Orijel et al. 2009; Kong et al. 2018; Corona-González 2019). Craterellus confluens was described by Berkeley (1867) from Mexico (Orizaba at central Veracruz state), in a locality relatively close to one of the current study sites in the Municipality of Zentla, Veracruz. Particularly from this latter region, an edible yellow chantherelle species common in the surrounding Quercus forest and even sold in popular markets, was previously reported under C. odoratus, considering it contaxic with Cr. confluens (Guzmán and Sampieri 1984; Guzmán 1985).

During a systematic multiyear sampling of basidiomes, as part of a project focused to study ectomycorrhizal fungi in tropical Quercus forests in eastern Mexico (Montoya et al. 2019a, b), we found coexisting three species of yellow Cantharellus. Two of these taxa are distinctive by having short-sized basidiomes with veined to gill-like folded hymenophore, while a third one, is distinctive by its medium-sized, moderately robust basidiomes, with smooth or at times rugulose hymenophore, this latter apparently corresponding to what was earlier reported as “C. odoratus”.

We report here the results of both, a morphological study of fresh specimens and a phylogenetic analysis of the transcription elongation factor 1-alpha (tef-1α) and the large subunit of the ribosome (nLSU) sequences obtained from our recent collections and those available in GeneBank. Three well-supported clades inferred in the phylogenetic tree, allowed us to recognize two new species and the record in Mexico of C. tabernensis, described from Southern Mississippi in USA (Feibelman et al. 1996). One of the new species here proposed, corresponds to the yellow Cantharellus with smooth hymenophore, which interestingly, in our phylogenetic analysis appears independent of Craterellus confluens (holotype), Cantharellus lateritius (holotype) and C. flavolateritius Buyck & V. Hofst. (paratype) sequences. Both macromorphological and color variation mentioned in the descriptions were recovered from fresh basidiomes through seven years of sampling. The monitoring of monodominant stands of three different species of tropical Quercus, allowed registering also, the putative ectomycorrhizal interaction of the studied species of Cantharellus.

Materials and methods

Sampling and morphological study

Cantharellus basidiomes were collected through a weekly sampling during June-October 2015–2018, with sporadic collections among 2009–2014, in tropical oak forests from Municipalities of both Zentla (837–850 m a.s.l.) and Alto Lucero (400–500 m a.s.l.) in central Veracruz (eastern Mexico). In these oak forests, Quercus oleoides is dominant, and even forming pure stands. In the Zentla locality, Q. glaucescens and Q. sapotifolia are also present, and form monodominant small stands. Descriptions are derived from recording the morpho-anatomical features of fresh samples, the records of color follow Kornerup and Wanscher (1978) (e.g. 4A4–8) and Munsell (1994) (e.g. 2.5Y 7/8–8/8). Basidiomes were dried in a hot air dehydrator (45 °C) for their preservation. Microscopic features were examined from desiccated specimens, measured in 3% KOH and stained with 1% Congo red or analyzed in Melzer´s solution. Thirty-five basidiospores per collection were measured in lateral view following Montoya et al. (2019b). In the descriptions X– denotes an interval of mean values of basidiospores length and width per collection in n collections, and Q– refers to the range of coef. Q (where Q is the average of the ratio of basidiospore length/basidiospore width in each collection). Line drawings were made with the aid of a drawing tube. Collections form part of XAL Herbarium (Thiers B. [continuously updated] Index Herbariorum: a global directory of public herbaria and associate staff. New York Botanical Garden`s Virtual Herbarium. http://sweetgum.nybg.org/science/ih/).

DNA extraction, PCR and sequencing

Genomic DNA was isolated from fresh material according to Cesar et al. (2018). We amplified the transcription elongation factor 1-alpha (tef-1α) using the pairs of primers tef-1F/tef-1R (Morehouse et al. 2003) and tef-1Fcanth/tef-1Rcanth (Buyck et al. 2014). We amplified the large subunit of ribosome (nLSU) using combinations of the pair of primers LR0R/LR7 (Vilgalys and Hesler 1990) and the pair of primers designed LRCA1(5'-GTTGCACTGTCCGAGTTGTA-3')/LRCA2(5'-AGACTGATGGCGAGGTATGA-3'). PCR was performed according to Herrera et al. (2018). A capillary sequencer, Genetic Analyzer 3730XL (Applied Biosystems), was used to obtain the sequences of the amplified PCR products. These sequences were assembled, edited, and deposited at GenBank database (Benson et al. 2017), the accession numbers are indicated in Table 1.

Table 1.

Cantharellus taxa: Fungal names, specimen vouchers, locations and GenBank accession numbers (for 28S and tef-1α). Newly sequenced collections in bold.

Taxa Voucher Locality LSU tef–1α
Cantharellus addaiensis BB 98.033 TYPE Tanzania KF294667 JX192992
Cantharellus afrocibarius BB 96.235 TYPE Zambia KF294668 JX192993
Cantharellus albidolutescens BB 08.070 TYPE Madagascar KF294646 JX192982
Cantharellus alborufescens BB 12.075 Italy KX929161 KX907243
BB 12.076 Italy KX907222 KX907244
Cantharellus altipes BB 07.019 TYPE USA KF294627 GQ914939
Cantharellus ambohitantelyensis BB 08.336 TYPE Madagascar KF294656 JX192989
Cantharellus amethysteus AH44796 TYPE Spain KR677550 KX828819
Cantharellus appalachiensis BB 07.123 USA KF294635 GQ914979
Cantharellus camphoratus TENN:F-38025 TYPE Canada KX896788
Cantharellus cerinoalbus AV 06.051 TYPE Malaysia KF294663
Cantharellus cibarius BIO10986 TYPE Sweden KR677539 KX828823
Cantharellus cinnabarinus BB 07.001 TYPE USA KF294624 GQ914985
Cantharellus coccolobae 1065/RC 11.25 TYPE Guadeloupe KX857089 KX857021
Cantharellus congolensis BB 98.039 Tanzania KF294609 JX193015
BB 98.058 Tanzania KF294673 JX192996
Cantharellus corallinus JJ MO-Canth-2 TYPE USA KX896776 KX857031
Cantharellus decolorans BB 08.278 TYPE Madagascar KF294654 GQ914968
Cantharellus enelensis 13.08.21.av02 TYPE Canada KX592712
Cantharellus ferruginascens BB 07.283 Slovakia KF294638 GQ914952
Cantharellus fistulosus DT 43 TYPE Tanzania KF294674 JX192992
Cantharellus flavolateritius Halling 6252 USA MT371334
JJ/NC-CANT-2 USA KX896783 KX857027
Cantharellus flavus C066WI TYPE USA JX030437
Cantharellus formosus SAR220712 Canada KR677553 KX828830
Cantharellus friesii AH44798 Spain KR677522 KX828831
Cantharellus garnierii RF32 PC TYPE New Caledonia AY392767
Cantharellus gracilis BB 98.234 TYPE Tanzania KF294612 JX192970
Cantharellus guyanensis 1501/MRG07 Guyane KX857094 KX857060
1517/MR Guyane KX857095 KX857061
Cantharellus hainanensis N.K. Zeng2289 TYPE China KY407524 KY407536
Cantharellus heinemannianus BB 96.307 TYPE Zambia KF294665
Cantharellus humidicolus BB 98.036 TYPE Tanzania KF294666 JX193005
Cantharellus ibityensis BB 08.196 TYPE Madagascar KF294650 GQ914980
Cantharellus isabellinus var. parvisporus BB 98.020 TYPE Tanzania KF294614 JX192972
Cantharellus iuventateviridis BP Looney 523 TYPE USA NG_060428 KX857047
Cantharellus lateritius TJ Baroni 8059F USA MT371335
TJ Baroni 8117L USA MT371336
BB 07.025 TYPE USA KF294633 GQ914959
Cantharellus lewisii BB 07.003 TYPE USA JN940597 GQ914962
Cantharellus lilacinopruinatus BB 07.221 Slovakia KF294637 GQ914951
Cantharellus miniatescens 1683/TH9870 Cameroon KX857108 KX857079
Cantharellus minor BB 07.002 USA KF294625 JX192978
BB 07.057 USA KF294632 JX192979
Cantharellus miomboensis BB 98.021 TYPE Tanzania KF294613 JX192971
Cantharellus pallens BB 09.441 Italy KX907218 KX907240
BB 12.082 Italy KX857092 KX857036
Cantharellus parvoflavus Montoya 5423 TYPE Mexico MT371337 MT449706
Herrera 204 Mexico MT371338 MT449707
Herrera 229 Mexico MT371339 MT449708
Cantharellus paucifurcatus BB 08.320 TYPE Madagascar KF294655 JX192988
Cantharellus phasmatis C073WI TYPE USA JX030426
Cantharellus platyphyllus BB 98.126 TYPE Tanzania KF294620 JX192975
Cantharellus platyphyllus subsp. bojeriensis BB 08.160 Madagascar KF294648 JX192984
Cantharellus pseudominimus JV 00.663 France KF294657 JX192991
Cantharellus quercophilus BB 07.097 TYPE USA KF294644 JX192981
Cantharellus romagnesianus AH44218 Spain KX828807 KX828836
Cantharellus roseofagetorum AH44789 TYPE Georgia NG_058962 KX828839
Cantharellus sebosus BB 08.234 TYPE Madagascar KF294652 JX192986
Cantharellus spectaculus C081WI TYPE USA JX030421 JX030414
Cantharellus splendens BB 96.306 TYPE Zambia KF294670
Cantharellus subalbidus BB 13.014B USA KX896782 KX857038
Cantharellus subamethysteus DS 06.218 TYPE Malaysia KF294664
Cantharellus subcyanoxanthus BB 00.1137 TYPE Madagascar JX192990
Cantharellus subincarnatus subsp. rubrosalmoneus BB 06.080 TYPE Madagascar KF294601 JX192962
Cantharellus symoensii BB 98.113 TYPE Tanzania KF294619 JX192974
Cantharellus tabernensis Herrera 120 Mexico MT371340 MT449709
Herrera 121 Mexico MT371341 MT449710
BB 07.056 TYPE USA KF294631 GQ914974
Cantharellus tanzanicus BB 98.040 TYPE Tanzania KF294622 JX192977
Cantharellus tenuithrix BB 07.125 TYPE USA JN940600 GQ914947
Cantharellus texensis BB 07.018 TYPE USA KF294626 GQ914988
Cantharellus tomentosus BB 98.060 TYPE Tanzania KF294672 JX192995
Cantharellus veraecrucis Herrera 142 Mexico MT371342
Herrera 58 Mexico MT371343 MT449711
Bandala 4505 TYPE Mexico MT371344 MT449712
Cantharellus violaceovinosus Corona 648 TYPE Mexico NG_064465 MF616521
Craterellus confluens Botteri 6 TYPE Mexico MT371345
Craterellus tubaeformis BB 07.293 Slovakia KF294640 GQ914989
Hydnum repandum BB 07.341 Slovakia KF294643 JX192980

Phylogenetic analysis

We constructed a concatenated dataset, using PhyDE v.0.9971 (Müller et al. 2010), with 19 sequences obtained here (nLSU and tef-1α) (Table 1), together with sequences of related taxonomic groups, and additionally taking as reference works on chantarelles by An et al. (2017), Buyck et al. (2014, 2016a, b, c, d), Herrera et al. (2018) and Olariaga et al. (2017). The dataset was aligned with MAFFT online service (Katoh et al. 2019), and the inconsistencies were corrected manually. Phylogenetic trees were generated according to Montoya et al. (2019a). The evolutionary model was calculated using the IQ-Tree 2.0-rc1 (Minh et al. 2020; Kalyaanamoorthy et al. 2017) and the best-fit model selected using the Bayesian Information Criterion (BIC), the Akaike Information Criterion (AIC) and corrected AIC. This later was used to generate a phylogenetic tree with the Maximum Likelihood (ML) method, with a Nearest Neighbour Interchange (NNI) heuristic, with the TIMe+I+G4 evolutionary model. A consensus tree was also generated calculating the Robinson-Foulds distance between the ML tree and the consensus tree, the branches being tested by means of Ultrafast Approach Bootstrap (UFBoot), SH-like approximate Likelihood Ratio Test (SH-aLRT), Approximate Bayes test (aBayes) and Bootstrap Standard (BS). Another phylogenetic tree (not shown) was also generated by Bayesian Inference (BI), using Mr Bayes v. 3.2.7 (Ronquist et al. 2012) according to Montoya et al. (2019a), with the previously calculated evolutionary model. The phylogenies from ML and BI analyses were displayed using FigTree v1.4.4 (Rambaut 2018). Only bootstrap values (BS) of ≥ 70% and Bayesian posterior probabilities (BPP) of ≥ 0.90 were considered and indicated on the tree branches (BS/BPP) of Fig. 1.

Figure 1. 

Phylogenetic relationships within Cantharellus species inferred from the combined nLSU (large subunit of the ribosome) and tef-1α (transcription elongation factor 1-alpha) sequences, by maximum likelihood method and Bayesian inference. The new species are indicated in bold letters. Bootstrap scores (only values ≥ 70) and Bayesian Posterior Probabilities (only values ≥ 0.90) are indicated above branches.

Results

We studied 78 specimens in the field (not all conserved) of Cantharellus species, each with basidiomes in different growth stages, most of them showing an annual fruiting pattern between August-October. We generated 19 new DNA sequences from eight fresh specimens and four from desiccated herbarium collections, twelve from nLSU and seven from tef-1α (Table 1). The built dataset includes a total of 80 sequences, using Craterellus tubaeformis and Hydnum repandum sequences as the outgroup (Table 1); the alignment is deposited in TreeBASE as 26146. In the inferred molecular phylogeny two groups of the produced sequences clustered in isolated position. One of them, the Cantharellus with smooth hymenophore, showed relationships with Craterellus confluens, Cantharellus lateritius and C. flavolateritius, and the other group appeared close to C. minor and C. romagnesianus (Fig. 1). Based on the distinctive morphological features and color variation of specimens of two clades, as well as their isolated position in the phylogeny obtained, we concluded that these Mexican specimens represent two distinct species, which are proposed here as new to science (described below). A third group of sequences clustered with strong support together with sequences of C. tabernensis type specimen (Fig. 1). Although Mexican samples, in contrast with the morphological description by Feibelman et al. (1996) that shows some minor differences (below discussed), all share the taxonomic distinctive characters to interpret them as being conspecific. In the classification proposed by Buyck et al. (2014) the Cantharellus with smooth hymenophore, clustered within subgenus Cantharellus and the other new species proposed here, together with C. tabernensis, within subgenus Parvocantharellus.

Taxonomy

Description of the new species

Cantharellus veraecrucis Bandala, Montoya & M. Herrera, sp. nov.

MycoBank No: 838105
Figs 2a, b, 3

Holotype

Mexico. Veracruz: Municipality of Zentla, around town of Zentla, 850 m a.s.l., gregarious on ground, under Quercus oleoides Schltdl. & Cham., 5 July 2012, Bandala 4505 (XAL).

Diagnosis

Differing from other related yellow Cantharellus species (subgenus Cantharellus) by the smooth hymenophore, often rugulose or with low, close, fine, irregular veins, pinkish-yellow, ellipsoid basidiospores 7–9 (–10.5) × (4.5–) 5–6.5 µm [Q–= 1.36–1.65], basidia (43–) 49–96 (–104) × 5–12 µm, pileipellis terminal hyphae 22–60 (–73) × 4–5.5 µm, subcylindrical, rarely subventricose, straight to moderately flexuous, wall ≤ 1 µm thick.

Figure 2. 

Basidiomes of Cantharellus species a, b C. veraecrucis (a Bandala 4505, holotype b Herrera 142) c, d C. parvoflavus (c Montoya 5423, holotype d Herrera 229) e, f C. tabernensis (e Herrera 120 f Herrera 131). Scale bars: 10 mm.

Gene sequences ex-holotype

nLSU MT371344; tef-1α MT449712.

Etymology

Referring to the locality of origin, in the State of Veracruz, Mexico.

Description

Pileus 20–80 (–100) mm diam, convex to plane convex, then more or less applanate and centrally depressed, becoming concave and finally broadly infundibuliform; involute margin when young, later incurved and becoming recurved or plane or uplifted in old specimens, not striate, at first entire, becoming variably lobed and undulate; surface dry, when young with appressed fibrils forming a moderately fine, squamulose surface especially at the center, smooth to glabrescent with age, yellow, light yellow (2.5Y 8/3, 7/12, 10YR 4–5/2), pale orange to bright yellow-orange (3A7–8, 4A4–8, 5A4–8, 2.5Y 7/8–8/8, 10YR 6/8, 7/6–8, 8/8) and even brownish-orange (5B7), at times light gray (10YR 7/1–2, 7.5YR 7/1, 4B2) at the center, orange-buff (5B5), salmon-orange to dirty peach-orange (6A6, 6B3, 6B5) or even brown (6E5). Hymenophore decurrent, smooth overall, often rugulose or with low, close, simple or forked, fine, irregular veins; paler than the pileus, light rose (10YR 8/2–3;7.5YR 7/3–4, 8/4, 5A2–4) when young although with age still preserving pinkish tints on a pale yellow (4A2–3), light yellow (10YR 8/3–4, 8/6, 2.5Y 8/4), light orange (6A3–4), or even egg yellow (4A8) ground. Stipe 10–75 × 6–21 mm, equal, tapering gradually downwards, somewhat sinuous or curved, central, occasionally somewhat eccentric, solid, glabrous to subtomentose, at times with age the surface becomes detached in scattered fibrils concolorous with hymenophore, whitish with yellow tinges (4A3–4), pale to bright yellow (4A6–8), orange (5A4), to orange-brown tinges (4A8, 4B7–8, 5B7) especially towards the base, often staining ochraceous or rusty orange color when handle; base in some specimens villous to finely villous under lens. Context fleshy, fibrous in stipe, concolorous with pileus or paler, yellowish-buff, odor agreeable fruity, faintly to peach or somewhat recalling butter; taste mild, fruity agreeable, finally somewhat bitter. KOH 3% negative, only somewhat orange on pileus, NH4OH 10% negative.

Figure 3. 

Cantharellus veraecrucis (Bandala 4505, holotype) a basidiospores b terminal elements of the pileipellis c basidia d longitudinal section of pileipellis. Scale bars: 5 μm (a); 10 μm (b, c); 25 μm (d).

Basidiospores 7–9 (–10.5) × (4.5–) 5–6.5 µm [X– = 7.8–8.9 × 5.3–6.1 µm, Q– = 1.36–1.65 (n = 12)], ellipsoid, smooth, thin-walled, hyaline, inamyloid. Basidia (43–) 49–96 (–104) × 5–12 µm, narrowly clavate to subcylindrical, with 2–5 sterigmata, thin-walled, hyaline, subhymenium composed of cylindrical hyphae 3–5 µm diam. Cystidia absent. Pileipellis a cutis composed of cylindrical hyphae 4–6 µm diam., intermingled in a compact arrangement, hyaline, yellowish colored in group; terminal hyphae 22–60 (–73) × 4–5.5 µm, subcylindrical, rarely subventricose, scattered, straight to moderately flexuous, smooth, hyaline, inamyloid, thick-walled (<1 µm thick). Pileus trama composed of cylindrical hyphae, 4–5 µm diam, slightly thick-walled (<1 µm thick), hyaline, some with weakly refringent contents. Hymenophoral trama composed of hyphae 4–5 µm diam, thin-walled, some with weakly refringent contents. Clamp connections present in all tissues.

Habitat

Solitary to gregarious, on soil, in tropical oak forest, in the studied sites it is recorded frequently in monodominant stands of Quercus oleoides, being less frequent in monodominant stands of Q. glaucescens Bonpl. or Q. sapotifolia Liebm.; fruiting in June-October at the coastal plain of central Veracruz State, east coast of Mexico.

Specimens examined

Mexico. Veracruz, Municipality of Zentla, Road Puentecilla-La Piña, 837 m a.s.l., 2 Jul 2009, Ramos 192, 193, 194; 21 Jun 2012, Herrera 23, 24, 28; 5 Jul 2012, Corona 649, 650, 653; 31 Jul 2012, Montoya 4887; 6 Nov 2013, Herrera 68. Around town of Zentla, 850 m a.s.l., 26 Jun 2013, Herrera 58, 59; 15 Jun 2016, Herrera 153, 154; 23 Jun 2016, Herrera 156; 6 Jul 2016, Herrera 175, 181, 183; 12 Jul 2016, Caro 71, Herrera 185, 186, 188, 190; 10 Aug 2016, Herrera 193; 5 Oct 2016, Melecio 16; 23 Oct 2018, Herrera 159; 12 Jul 2017, Montoya 5347; 21 Sep 2017, Garay 394, Garrido 88, 89; 20 Jun 2018, Herrera 232. Municipality of Alto Lucero, NE Mesa de Venticuatro, 450–500 m a.s.l., 17 Sep 2015, Herrera 140; 4 Sep 2018, Herrera 244. Jaguarundi Park, Coatzacoalcos 29 Sep 2015, Herrera 142, 143, 144, 145 (all at XAL).

Remarks

Cantharellus veraecrucis is distinguished by the basidiome colors, hymenophore smooth (or at times discontinuously rugulose) with pinkish tinges, and pileus surface with appressed fibrils. In some stage of development, it superficially might look like C. flavolateritius; this latter, however, according to Buyck et al. (2016a) exhibits bright yellow colors on pileus, the hymenophore is composed of radially oriented, low anastomosing veins,“… locally almost smooth…”, paler stipe (yellow to off-white), narrowly ellipsoid, somewhat phaseoliform basidiospores (7.1–) 7.2–7.88–8.5 (–10.0) × (4.0–) 4.2– 4.71–5.2 (–5.8) μm, Q = (1.4–) 1.5–1.69–1.8 (–2.1) and pileipellis terminal hyphae often rather short, clavulate or apically slightly inflated, rarely ellipsoid, mostly 20–50 (–70) μm long, sometimes more or less wavy-undulate in outline.

In our phylogenetic analysis, C. veraecrucis is related also with C. lateritius. This latter species exhibits pale to deep yellow or even apricot orange (Buyck et al. 2011) or bright orange or slightly pinkish orange colors (Petersen 1979). Buyck et al. (2011) with their field experience also cited that C. lateritius “… has an often excentrical, sometimes laterally compressed, short to long, more or less yellow stipe that can remain white at the base but is concolorous with the cap higher up, and it has an almost smooth to clearly veined often slightly pinkish tinted hymenophore (the senior author has never seen absolutely smooth specimens)...”. Based on our revision of the epitype of C. lateritius (Buyck 07.025 kept at PC, designated by Buyck and Hofstetter 2011), it microscopically differs from C. veraecrucis by the basidiospores shape (ellipsoid to slightly phaseoliform) and the terminal hyphae of the pileipellis, which are (19–) 21–60 (–70) × 5–11 µm, cylindrical to subclavate, tending to be wider than those of C. veraecrucis (Fig. 7).

The Asian Cantharellus hiananensis N.K. Zeng, Zhi Q. Liang & S. Jiang, appears related also to C. veraecrucis, but according to data by An et al. (2017), it differs from the Mexican species by its smaller basidiome size (pileus 25–55 mm diam., stipe 30–55 × 8–10 mm), paler hymenophore (cream to yellowish white), stipe usually hollow covered with tiny, yellow to pale yellowish brown scales, smaller, subcylindrical basidiospores [6–7.09–8 (–9) × (4–) 4.5–4.84–5 (–5.5) µm], and smaller basidia (50–70 × 7–10 µm), (4–) 5 (–6) -spored and pileipellis terminal hyphae 23–82 × 3–8 mm, narrowly clavate or subcylindrical, sometimes subfusiform, with obtuse apex.

Cantharellus veraecrucis represents a wild edible mushroom that is harvested for consumption and commercialization during the rainy season, in the study site and surroundings; it is known as “Oak mushroom”. After our systematic multiyear sampling of basidiomes in the forests studied, we could observe that C. veraecrucis is a frequent chanterelle, and shares the same habit preferences as C. violaceovinosus, recently described from the same region (Herrera et al. 2018).

Cantharellus parvoflavus M. Herrera, Bandala & Montoya, sp. nov.

MycoBank No: 838106
Figs 2c, d, 4

Holotype

Mexico. Veracruz: Municipality of Alto Lucero, NE Mesa de Venticuatro, 450–500 m a.s.l. gregarious, on ground, under Quercus oleoides Schltdl. & Cham., 2 Oct 2017, Montoya 5423 (XAL).

Diagnosis

Differing from other related Cantharellus species (subgenus Parvocantharellus) by the pileus surface with appressed fibrils at center, broadly ellipsoid basidiospores 6–9 (–9.5) × 4.5–5 µm [Q–= 1.52–1.57 (n=3)], pileipellis terminal hyphae (23–) 25–75 (–80) µm × (3.5–) 4–8 µm, mostly cylindrical, often subclaviform, subventricose or somewhat narrowly utriform.

Gene sequences ex-holotype

nLSU MT371337; tef-1α MT449706.

Etymology

Referring to a small, yellow chanterelle; from parvus (Lat.): small and flavus (Lat.): yellow

Description

Pileus 6–26 mm diam, subhemispheric in young, becoming convex to plane convex and centrally depressed, some finally irregularly infundibuliform; margin incurved when young, becoming inflexed to somewhat straight, undulate or irregular or more or less crenate, not or obscurely translucid striate; surface dry, with appressed fibrils at center when young, glabrous at remaining areas, with waxy appearance, bright yellow-orange (5A5–A8) with tiny white to light yellow scales in the center when young, paler at edge when young. Hymenophore decurrent or shortly decurrent, with gill-like folds up to 2 mm deep, subdistant to more frequently distant, at times forked, moderately thick with margin entire or often irregular or eroded, frequently intervenose, some specimens (especially towards the stipe) with irregular low and sinuous veins, often with lower irregular anastomosis among the folds, in some specimens the anastomosis occur practically in the whole hymenophore, while in others only at some areas, especially at pileus margin, with some short lamellulae-like folds, concolorous with the pileus. Stipe (10–) 15–42 × 2–6 mm, broadened towards the apex, somewhat fused, compressed at times or furrowed, solid but soon fistulous to hollow, glabrous, concolorous with pileus. Context fleshy, concolorous with pileus or somewhat paler, with waxy appearance, odor mild, agreeable; taste mild, agreeable.

Basidiospores 6–9 (–9.5) × 4.5–5 µm [X– = 7.6–7.8 × 4.9–5 µm, Q– = 1.52–1.57 (n = 3)], broadly ellipsoid, smooth, thin-walled, hyaline, inamyloid, with granular contents or refractive droplets. Basidia 50–83 (–89) × (6–) 7–10 µm, narrowly clavate to subcylindrical, with 2–5 sterigmata, thin-walled, hyaline; subhymenium composed of cylindrical hyphae 4–6 µm diam. Cystidia absent. Pileipellis composed of intermingled hyphae of 4–7 µm diam, cylindrical, hyaline, yellowish in group, terminal hyphae (23–) 25– 75 (–80) × (3.5–) 4–8 µm, mostly cylindrical, often subclaviform, subventricose or somewhat narrowly utriform, moderately straight to flexuous, inamyloid, thick-walled (<1 µm thick), smooth, hyaline. Pileus trama composed of cylindrical to inflated hyphae, 4–7 µm diam, slightly thick-walled (<1 µm thick), hyaline, some with weakly refringent contents. Hymenophoral trama composed of hyphae 4–5 µm diam, thin-walled, some with weakly refringent contents. Clamp connections present in all tissues.

Figure 4. 

Cantharellus parvoflavus (Montoya 5423, holotype) a basidiospores b Terminal elements of the pileipellis c basidia d longitudinal section of pileipellis. Scale bars: 5 μm (a); 10 μm (b, c); 25 μm (d).

Habitat

Solitary to gregarious, rare in the study area, on soil, in tropical oak forest, under Quercus oleoides, September-October, known in the coastal plain of central Veracruz State, east coast of Mexico.

Specimens examined

Mexico. Veracruz, Municipality of Alto Lucero, NE Mesa de Venticuatro, 392–433 m a.s.l., 27 Sep 2016, Herrera 204; 20 Oct 2017, Herrera 229 (all at XAL).

Remarks

The phylogenetic analysis supports (with high values of bootstrap and Bayesian posterior probabilities 100/1) the distinction of Cantharellus parvoflavus as a new species, sister to C. appalachiensis from USA. This latter species, besides their basidiomes being somewhat larger [pileus 10–50 mm/stipe 15–75 × 3–10 (–13) mm], are not distinctly yellow, only dingy yellow, usually dull brown, pale or yellowish-brown at margin, darker to brown on disc (Petersen and Ryvarden 1971; Bigelow 1978). Moreover, C. appalachiensis has wider broadly-ellipsoid basidiospores [(6.6–) 7.4–8.2 (–8.9) × (4.4–) 4.8–5.6 (–5.9) µm or (6–) 7.5–9 (–10.5) × (4–) 4.5–5.5 (–6) µm] and wider pileipellis hyphae (3–14.5 µm diam. or 9–14 µm diam.) (Petersen and Ryvarden 1971; Bigelow 1978).

Cantharellus parvoflavus is similar to yellow forms of C. minor, because they have a hygrophoroid appearance, but this latter is usually bright yellow orange to orange, fading to pale orange-buff or pale orange, with glabrous pileus surface, bigger, ellipsoid, slightly phaseoliform basidiospores (6–) 7.5–10 (–11.5) × (4–) 4.5–6 (–6.5) µm and pileipellis terminal elements subcylindrical to subventricose (Bigelow 1978; Buyck et al. 2010). Cantharellus romagnesianus is close to C. parvoflavus but it develops grey-brown colors in the pileus, its hymenophore has forked veins, often spaced, larger basidiospores [(8–) 9–11.5 (–12.5) × 4–6 (–6.5), Q = 1.71–2.28] and with different shape (Olariaga et al. 2017).

New record of Cantharellus tabernensis in Mexico

Cantharellus tabernensis Feib. & Cibula, Mycologia 88: 299 (1996)

Figs 2e, f, 5

Description

Pileus 10–30 mm diam, hemispheric to convex, becoming broadly conical to plane convex and faintly depressed in the disc, margin incurved when young, somewhat inflexed to straight with age or somewhat reflexed, not striate, not or faintly undulate or crenulate; hygrophanous, with dull appearance, some with greyish appressed fibrils at center and smooth at the margin when young, smooth to glabrescent with age; light yellow (2.5Y 8/6–8/8, 4A5). Hymenophore decurrent or shortly decurrent, with gills up to 3 mm deep, subdistant to more frequently distant, continuous, or forked at different levels, moderately thick; margin entire, at times with irregular anastomosis among folds, with short lamellulae-like folds; yellow to egg yellow (10YR 8/8) brighter than the pileus. Stipe (15–) 19–40 × 2–6 mm, central or at times slightly eccentric, equal, occasionally somewhat applanate, at times slightly fused or broader at base, solid to hollow, often furrowed especially below, hygrophanous, surface smooth, concolorous with the pileus; mycelium whitish to pale yellowish. Context 1–3 mm thick cream color to yellowish, odor mild, agreeable; taste mild, agreeable.

Basidiospores 6.5–8.5 × 4.5–5 µm [X– = 7.32–7.34 × 4.8–4.9 µm, X– = 1.49–1.52, (n = 2)], ellipsoid, smooth, thin-walled, hyaline, inamyloid, with granular contents or refractive droplets. Basidia (53–) 56–87 (–99) × 6–10 µm, narrowly clavate to subcylindrical, with 2–4 sterigmata, thin-walled, hyaline; subhymenium composed of cylindrical hyphae 3–5 µm diam. Cystidia absent. Pileipellis a cutis composed of hyphae 5–8 µm diam, intermingled in a compact arrangement, cylindrical, hyaline, inamyloid, with terminal hyphae cylindrical to somewhat subclavate, 62–75 × 6–10 µm, slightly thick-walled (<1 µm thick), smooth, hyaline, inamyloid, usually abundant. Pileus trama composed of cylindrical hyphae, 3–8 µm diam, slightly thick-walled (<1 µm thick), hyaline. Hymenophoral trama composed of hyphae 3–6 µm diam, thin-walled. Clamp connections present in all tissues.

Figure 5. 

Cantharellus tabernensis (Herrera 131) a basidiospores b basidia c Terminal elements of the pileipellis d longitudinal section of pileipellis. Scale bars: 5 μm (a); 10 μm (b, c); 25 μm (d).

Habitat

Solitary to gregarious, rare in the study area, on soil, in tropical oak forest, under Quercus oleoides and Q. sapotifolia, fruiting in June at the coastal plain of central Veracruz State, east coast of Mexico.

Specimens examined

Mexico. Veracruz, Municipality of Zentla, Road Puentecilla-La Piña, 837 m a.s.l.,11 Jun 2015, Herrera 120, 121;10 Sep 2015, Herrera 131 (all at XAL).

Remarks

In our phylogeny Mexican sequences of specimens Herrera 120 and 121 clustered (Fig. 1) with high values of Bootstrap and Bayesian posterior probabilities (96/0.99) with a sequence of the type specimen of Cantharellus tabernensis from U.S.A., produced by Buyck et al. (2014). The morphological description provided above includes both mentioned specimens, and in fact, in the most relevant characters, those specimens agree with the species. It should be mentioned, however, that the following features recorded in the description provided by Feibelman et al. (1996) were not observed in the Mexican material: pileus mat felted overall, often umbilicate, sometimes perforated, basidia 4–5–6 -spored and dark plasmatic pigment confined to clavate terminal cells of the surface hyphae at disc.

The record presented here of C. tabernensis, in its turn provides additional information on the species distribution. It is known from the mixed pine and hardwood forests, usually near Pinus elliotii Engelm., at the Gulf coastal plain in Texas, Mississippi, and Louisiana states in USA (Feibelman et al. 1996), and now C. tabernensis is known also in the tropical Quercus forest from Veracruz, in the coastal plain of Veracruz state in the Gulf of Mexico.

Proposal of a new name for the replacement of Craterellus confluens

Derived from the fact that Craterellus confluens was described by Berkeley (1867) from the Orizaba region in Veracruz (Mexico), later records of yellow chantherelles occurring in the Zentla region (north of Orizaba) were referred to in the past by Guzmán and Sampieri (1984) as “Cantharellus odoratus” following Corner (1966). This latter author introduced that C. lateritius and Cr. confluens were the same as Schweinitz (1822) described as Merulius odoratus. Burt (1914) mentioned particularly the macroscopic resemblance among the Cr. confluens isotype specimen in Schweinitz herbarium and the specimens of C. odoratus that he studied, thus he synonymized the former and pointed out that “…the type of Cr. confluens has the hymenium rugose-wrinkled, as is often the case in specimens of C. odoratus; its habit, dimensions, structure, coloration, and spores are quite those of C. odoratus…”. In the molecular phylogeny here generated (Fig. 1) Cr. confluens holotype specimen (Botteri 6, kept at K) is supported with high values of bootstrap and Bayesian posterior probabilities sister to C. veraecrucis here described (above), and both are closely related with C. lateritius (including a sequence of the type) and C. flavolateritius. Petersen (1979) after type studies considered indeed, separately C. lateritius, Cr. odoratus (Schwein.) Fr. and Cr. confluens, being a combination of characters such as clamps (present or not), basidiome colors and the leathery, funnel-shaped basidiocarps (with a hollow stipe), among other features, considered in the distinction of such taxa. Molecular studies have also shown that Schweinitz’s species belongs to Craterellus (i.e. Cr. odoratus) (Feibelman et al. 1997; Dahlman et al. 2000) and now, our analysis confirms (Fig. 1) that Cr. confluens holotype specimen belongs to Cantharellus, among the group of yellow species around C. lateritius. Buyck and Hofstetter (2011) suggested “…to refrain from using the name C. confluens any longer…”, but rather a new specific name in Cantharellus is required for such taxon because in Cantharellus the specific name is preoccupied by C. confluens (Schwein.) Schwein. 1834, i.e. Merulius confluens Schwein. 1822, a meruliod species (Burt 1917) member of Byssomerulius (Ginns 1975; Zmitrovich et al. 2006). Possibly Cr. confluens exhibits a rare occurrence in the site that we explored in the Zentla region or it has a more restricted occurrence in some other ecosystem, near or around the city of Orizaba, Veracruz. Considering the features of the fruitbodies (“…stem divided…”) mentioned by Berkeley (1867) in the diagnosis, we propose to replace the name as follows:

Cantharellus furcatus Bandala, Montoya & Ramos, nom. nov.

MycoBank No: 838107

Bas. Craterellus confluens Berk. & M.A. Curtis, J. Linn. Soc., Bot. 9: 423 (1867).

Syn. Cantharellus confluens (Berk. & M.A. Curtis) R.H. Petersen, Sydowia 32: 201 (1979) nom. illeg.

Non Cantharellus confluens (Schwein.) Schwein., Trans. Am. Phil. Soc., New Series 4: 153 (1834).

= Merulius confluens Schwein., Schr. Nat. Ges. Leipzig 1: 92 (66 in reprint) (1822).

= Byssomerulius corium (Pers.: Fr.) Parmasto, Eesti NSV Tead. Akad. Toim., ser Biol. 16: 383 (1967).

Holotype

Mexico. Veracruz, Orizaba. Botteri 6 [ex herb. M.J. Berkeley] KM 173247 (K).

Gene sequences ex-holotype

nLSU MT371345.

Etymology

From furcatus (Lat.): forked, referring to a bifurcation developed in the basidiome.

Remarks

Presumably having been separated from the entire collection, the holotype specimen consists of a single unipileate basidiome but the diagnostic feature mentioned by Berkeley (1867) “… stem divided above into numerous pilei…”, a feature practically not observed in close related species (C. flavolateritius, C. lateritius, C. veraecrucis) is present, as noted and depicted by Burt (1914), in the isotype collection at Farlow Herbarium (https://huh.harvard.edu/pages/farlow-herbarium-fh), and it is well-depicted and described for collections from SE USA studied by Petersen (1979). The particularity of producing multipileate basidiomes and/or with fused stipes, in combination with the smooth pileus surface, pileus and hymenophore predominantly orange colored (aurantiacus in the diagnosis) hymenophore rugulose, irregularly forking and anastomosing, rarely smooth, with yellow stipe and lacking pinkish shades (Petersen 1979), are the distinctive macroscopic features of C. furcatus.

The holotype specimen Botteri 6 (at K) of Cr. confluens was preserved in such a poor condition that it does not allow a proper rehydration of the tissues. The microscopic features recovered were: basidiospores of 7.5–8.5 × 5–6 µm (X– = 7.8 × 5.3 µm), Q– = 1.46, broadly ellipsoid to ellipsoid, some subglobose, somewhat flattened adaxially, smooth, hyaline, thin-walled, inamyloid. Pileipellis a cutis composed of cylindrical hyphae 5–7 µm diam, compactly arranged, hyaline, yellowish colored in group; terminal hyphae 36–57 × 8–12 µm, clavate to broadly clavate, scattered, smooth, hyaline, inamyloid, thin to thick-walled (<1 µm thick). Clamp connections present (Fig. 6). In the holotype Petersen (1979) registered basidiospores of 6.7–8.9 × 4.8–5.9 µm, Q– = 1.29–1.54 and of 7–10 × 5–6.3 µm, while in the isotype collection there is an annotation made in 1980 by Dr. H.E. Bigelow, describing basidiospores: 8–10 × 5.5–6.5 µm, ellipsoid or broadly ellipsoid or subglobose, smooth, inamyloid, basidia mostly collapsed, ± 41–52 × 6–7.5 µm, pileus with hyphae 4–10 µm diam, clamped, pigment apparently intracellular (https://huh.harvard.edu/pages/farlow-herbarium-fh).

Figure 6. 

Cantharellus furcatus (Botteri 6, holotype of Craterellus confluens) a basidiospores b terminal elements of the pileipellis. Scale bars: 5 μm (a); 10 μm (b).

Figure 7. 

Terminal elements of the pileipellis of Cantharellus species a, b C. lateritius (a Buyck 05.058 b Buyck 07.025 epitype) c C. veraecrucis (Bandala 4505, holotype). Scale bar: 10 μm.

Acknowledgements

This research was supported by CONACYT (CB 252431); we also acknowledge support given by CONACYT (225382) to the Laboratorio de Presecuenciación, Red Biodiversidad y Sistemática, INECOL. M. Herrera appreciates the scholarship grant (261413). We acknowledge PC staff for the loan of herbarium specimens as well as K for the loan of the Botteri collection and permission for its molecular study. We extend our appreciation to Dr. R.E. Halling (NYBG) and Dr. T.J. Baroni (SUNY, Cortland) for the loan of Cantharellus specimens from USA. Assistance in the field and laboratory was provided by D. Ramos (Instituto de Ecología, A.C.) and B. Pérez (INECOL) assisted us with some molecular procedures.

References

  • An D-Y, Liang Z-Q, Jiang S, Su M-S, Zeng N-K (2017) Cantharellus hiananensis, a new species with a smooth hymenophore from tropical China. Mycoscience 58: 438–444. https://doi.org/10.1016/j.myc.2017.06.004
  • Arora D, Dunham SM (2008) A new, commercially valuable chantharelle species, Cantharellus californicus sp. nov., associated with live oak in California. Economic Botany 62: 376–391. https://doi.org/10.1007/s12231-008-9042-7
  • Burt EA (1914) The Thelephoraceae of North America. II. Craterellus. Annals of the Missouri Botanical Garden 1(3): 327–350. https://doi.org/10.2307/2990079
  • Buyck B, Lewis DP, Eyssartier G, Hofstetter V (2010) Cantharellus quercophilus sp. nov. and its comparison to other small, yellow or brown American chanterelles. Cryptogamie, Mycologie 31(1): 17–33.
  • Buyck B, Hofstetter V (2011) The contribution of tef-1 sequences to species delimitation in the Cantharellus cibarius complex in the southeastern USA. Fungal Diversity 49(1): 35–46. https://doi.org/10.1007/s13225-011-0095-z
  • Buyck B, Cruaud C, Couloux A, Hofstetter V (2011) Cantharellus texensis sp. nov. from Texas, a Southern lookalike of C. cinnabarinus revealed by tef-1 sequence data. Mycologia 103(5): 1037–1046. https://doi.org/10.3852/10-261
  • Buyck B, Kauff F, Eyssartier G, Couloux A, Hofstetter V (2014) A multilocus phylogeny for worldwide Cantharellus (Cantharellales, Agaricomycetidae). Fungal Diversity 64(1): 101–121. https://doi.org/10.1007/s13225-013-0272-3
  • Buyck B, Oraliaga I, Justice J, Lewis D, Roody W, Hofstetter V (2016a) The dilemma of species recognition in the field when sequences data are not in phase with phenotypic variability. Cryptogamie, Mycologie 37(3): 367–389. https://doi.org/10.7872/crym/v37.iss3.2016.367
  • Buyck B, Moreau PA, Courteciusse R, Kong A, Roy M, Hofstetter V (2016b) Cantharellus coccolobae sp. nov. and Cantharellus garnierii, two tropical members of Cantharellus subg. Cinabarinus. Cryptogamie, Mycologie 37(3): 391–403. https://doi.org/10.7872/crym/v37.iss3.2016.391
  • Buyck B, Henkel TW, Dentinger BTM, Séné O, Hofstetter V (2016c) Multigene sequencing provides a suitable epitype, barcode sequences and a precise systematic position for the enigmatic, African Cantharellus miniatescens. Cryptogamie, Mycologie 37(3): 269–282. https://doi.org/10.7872/crym/v37.iss3.2016.269
  • Cesar E, Bandala VM, Montoya L, Ramos A (2018) A new Gymnopus species with rhizomorphs and its record as nesting material by birds (Tyrannidae) in the subtropical cloud forest from eastern Mexico. MycoKeys 42: 21–34. https://doi.org/10.3897/mycokeys.42.28894
  • Corner EJH (1966) A monograph of cantharelloid fungi. Oxford University Press, 255 pp.
  • Corona-González ShM (2019) Los hongos de los totonacos en Zongozotla, Puebla. Arqueología Mexicana, edición especial 87: 78–79.
  • Dahlman M, Danell E, Spatafora JW (2000) Molecular systematics of Craterellus: cladistic analysis of nuclear LSU rDNA sequence data. Mycological Research 104(4): 388–394. https://doi.org/10.1017/S0953756299001380
  • Feibelman TP, Doudrick RL, Cibulac WG, Bennett JW (1997) Phylogenetic relationships within the Cantharellaceae inferred from sequence analysis of the nuclear large subunit rDNA. Mycological Research 101(12): 1423–1430. https://doi.org/10.1017/S0953756297004115
  • Foltz MJ, Perez KE, Volk TJ (2013) Molecular phylogeny and morphology reveal three new species of Cantharellus within 20m of one another in western Wisconsin, USA. Mycologia 105(2): 447–461. https://doi.org/10.3852/12-181
  • Garibay-Orijel R (2009) Los nombres zapotecos de los hongos. Revista Mexicana de Micología 30: 43–61.
  • Ginns JH (1975) Merulius: s.s. and s.l., taxonomic disposition and identification of species. Canadian Journal Botany 54: 100–167. https://doi.org/10.1139/b76-014
  • Guevara G, Garza Ocañas F, Cázarez E (2004) Estudio del ITS nuclear en algunas especies del género Cantharellus de México. Ciencia UANL 7(3): 371–378.
  • Guzmán G (1985) Estudios sobre los Cantharelaceos de México (Fungi, Aphyllophorales). Biotica 10(4) 395–402.
  • Guzmán G, Sampieri A (1984) Nuevos datos sobre el hongo comestible Cantharellus odoratus en México. Boletín de la Sociedad Matemática Mexicana 19: 201–205.
  • Henkel T, Wilson AW, Aime MC, Dierks J, Uehling JW, Roy M, Schimann S, Wartchow F, Mueller GM (2014) Cantharellaceae of Guyana II: New species of Craterellus, new South American distribution records for Cantharellus guyanensis and Craterellus excelsus, and a key to the Neotropical taxa. Mycologia 106(2): 307–322. https://doi.org/10.3852/106.2.307
  • Kalyaanamoorthy S, Minh BQ, Wong TKF, Haeseler A, Jermiin LS (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods 14: 587–589. https://doi.org/10.1038/nmeth.4285
  • Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
  • Kong A, Montoya A, García-de Jesús S, Ramírez-Terrazo A, Andrade R, Ruan-Soto F, Rodríguez-Palma MM, Estrada-Torres A (2018) Hongos ectomicorrizógenos del Parque Nacional Lagunas de Montebello, Chiapas. Revista Mexicana de Biodiversidad 89: 741–756. https://doi.org/10.22201/ib.20078706e.2018.3.2527
  • Kornerup A, Wanscher JH (1978) Methuen Handbook of Colour (3rd edn). Methuen, London 252 pp.
  • Leacock PR, Riddell J, Wilson AW, Zhang R, Ning Ch, Mueller GM (2016) Cantharellus chicagoensis sp. nov. is supported by molecular and morphological analysis as a new yellow chanterelle in midwestern United States. Mycologia 108(4): 765–772. https://doi.org/10.3852/15-230
  • Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, von Haeseler A, Lanfear R (2020) IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology Evolution 37: 1530–1534. https://doi.org/10.1093/molbev/msaa015
  • Montoya L, Caro A, Ramos A, Bandala VM (2019a) Two new species of Lactifluus (Fungi, Russulales) from tropical Quercus forest in eastern Mexico. MycoKeys 59: 27–45. https://doi.org/10.3897/mycokeys.59.38359
  • Montoya L, Garay-Serrano E, Bandala VM (2019b) Two new species of Phylloporus (Fungi, Boletales) from tropical Quercus forests in eastern Mexico. MycoKeys 51: 107–123. https://doi.org/10.3897/mycokeys.51.33529
  • Morehouse EA, James TY, Ganley ARD, Vilgalys R, Berger L, Murphy PJ, Longcore JE (2003) Multilocus sequence typing suggests the chytrid pathogen of amphibians is a recently emerged clone. Molecular Ecology 12: 395–403. https://doi.org/10.1046/j.1365-294X.2003.01732.x
  • Müller J, Müller K, Neinhuis C, Quandt D (2010) PhyDE – Phylogenetic data editor, version 0.9971. Program distributed by the authors. http://www.phyde.de
  • Munsell soil Color Charts (1994) Munsell soil Color Charts. Macbeth, New Windsor, 10 pp. [9 pl.]
  • Olariaga I, Buyck B, Esteve-Raventós F, Hofstetter V, Manjón JL, Moreno G, Salcedo I (2015) Assessing the taxonomic identity of white and orange specimens of Cantharellus: occasional colour variants or independent species? Cryptogamie, Mycologie 36(4): 1–14. https://doi.org/10.7872/crym/v36.iss3.2015.287
  • Olariaga I, Moreno G, Manjón JL, Salcedo I, Hofstetter V, Rodríguez D, Buyck B (2017) Cantharellus (Cantharellales, Basidiomycota) revisited in Europe through a multigene phylogeny. Fungal Diversity 83(1): 263–292. https://doi.org/10.1007/s13225-016-0376-7
  • Pérez-Moreno J, Martínez-Reyes M, Yescas-Pérez A, Delgado-Alvarado A, Xoconostle-Cázares B (2008) Wild mushroom markets in Central Mexico and a case study at Ozumba. Economic Botany 62(3): 425–436. https://doi.org/10.1007/s12231-008-9043-6
  • Petersen RH, Ryvarden L (1971) Notes on cantharelloid fungi IV. Two new species of Cantharellus. Svensk Bot. Tidskr. 65: 399–405.
  • Petersen RH (1979) Notes on cantharelloid fungi X. Cantharellus confluens and C. lateritius, Craterellus odoratus and C. aureus. Sydowia 32: 198–208.
  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  • Thorn RG, Kim JI, Lebeur R, Voit A (2017) The golden chantherelles of Newfoundland and Labrador: a new species, a new record for North America, and a lost species rediscovered. Botany 95: 547–560. https://doi.org/10.1139/cjb-2016-0213
  • Vilgalys R, Hesler M (1990) Rapid genetic identification and mapping of enzimatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172(8): 4238–4246. https://doi.org/10.1128/JB.172.8.4238-4246.1990
  • Wilson A, Aime MC, Dierks J, Mueller G, Henkel T (2012) Cantharellaceae of Guyana I: new species, combinations and distribution records of Craterellus and a synopsis of known taxa. Mycologia 104(6): 1466–1477. https://doi.org/10.3852/11-412
  • Zmitrovich IV, Spirin WA, Wasser SP (2006) Variability of Byssomerulius corium in the Mediterranean. Mycotaxon 97: 83–90.