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Phylogeny- and morphology-based recognition of new species in the spider-parasitic genus Gibellula (Hypocreales, Cordycipitaceae) from Thailand
expand article infoWilawan Kuephadungphan, Kanoksri Tasanathai, Booppa Petcharad§, Artit Khonsanit, Marc Stadler|, J. Jennifer Luangsa-ard
‡ National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathum Thani, Thailand
§ Thammasat University, Pathumthani, Thailand
| Helmholtz Centre for Infection Research, Braunschweig, Germany
Open Access

Abstract

Thailand is known to be a part of what is called the Indo-Burma biodiversity hotspot, hosting a vast array of organisms across its diverse ecosystems. This is reflected by the increasing number of new species described over time, especially fungi. However, a very few fungal species from the specialized spider-parasitic genus Gibellula have ever been reported from this region. A survey of invertebrate-pathogenic fungi in Thailand over several decades has led to the discovery of a number of fungal specimens with affinities to this genus. Integration of morphological traits into multi-locus phylogenetic analysis uncovered four new species: G. cebrennini, G. fusiformispora, G. pigmentosinum, and G. scorpioides. All these appear to be exclusively linked with torrubiella-like sexual morphs with the presence of granulomanus-like asexual morph in G. pigmentosinum and G. cebrennini. A remarkably high host specificity of these new species towards their spider hosts was revealed, and for the first time, evidence is presented for manipulation of host behavior in G. scorpioides.

Keywords

Cordycipitaceae, Gibellula, spider specialist fungus, taxonomy

Introduction

To arthropodologists or even arachnologists, it is surprising that fungal pathogens of spiders seem to be generally neglected when the host can be completely overgrown by the pathogens to be unrecognizable as a spider. Nonetheless, this group of fungi has been known and studied for more than two centuries (Evans 2013). Recently, over 80 fungal species, mostly distributed in Cordycipitaceae, have been reported as spider pathogens (Shrestha et al. 2019). Among them, only Gibellula and Hevansia are obligate parasites of spiders whereas others appear to be natural enemies of different insects and do not show apparent host specificity.

Gibellula is well-known to be a specialized spider-parasitic genus widely distributed worldwide, mostly found in tropical regions (Shrestha et al. 2019). Originally, the type species, G pulchra (Sacc.) Cavara was known as Corethropsis pulchra Sacc. collected from Italy, recognized by producing primarily synnematous, aspergillus-like conidiophores with terminal vesicles, each gives rise to phialides produced on metulae (Saccardo 1877; Shrestha et al. 2019). After establishing a genus Gibellula Cavara in honor of Prof. Giuseppe Gibelli by Cavara (1894), a number of species in this genus were recorded across the world (Shrestha et al. 2019). Currently, nearly 40 species have been described and listed in the global fungal databases Index Fungorum (www.indexfungorum.org) and MycoBank (www.mycobank.org). According to the review of Shrestha et al. (2019), many of them including G arachnophila (Ditmar) Vuill., G arachnophila f. arachnophila (Ditmar) Vuill., G arachnophila f. macropus Vuill., G. aranearum P. Syd., G globosa Kobayasi & Shimizu, G globosostipitata Kobayasi & Shimizu, G haygarthii Van der Byl, G suffulta Speare and G tropicalis Sawada were synonymized with G pulchra whereas G arachnophila f. leiopus Vuill. ex. Maubl., G araneae Sawada and G perexigua (Kobayasi) Koval were synonymized with G leiopus (Vuill. ex Maubl.) Mains. In addition to these species, the identities of several other species reported in this genus still remain doubtful. Petch (1932) expressed uncertainty about the identities of G aspergilliformis (Rostr.) Vuill. and G phialobosia Penz. & Sacc. by pointing out that the narrow metulae and spherical conidia in chains present in G aspergilliformis and the flask-shaped phialides in the latter species were not common features of Gibellula. Moreover, description of G eximia Höhn. did not point to the genus. Since Gibellula is well-known as an obligate parasite of spiders, Mains (1950) reported that the assignment of G elegans Henn. to this genus might be erroneous, as this species is found occurring on locusts. According to Mains (1950), the description of G capillaris Morgan did not fit the concept of Gibellula and re-examination of the type specimen is unfortunately infeasible since it is no longer in a good condition. Tzean et al. (1997) doubted the identity of G araneicola Sawada that produces an isarioid morph instead of Gibellula. In the case of G. petchii Humber & Rombach, it is still unclear whether the species name should be retained or abandoned. Gibellula petchii Humber & Rombach was proposed to accommodate Cylindrophora aranearum Petch, which was originally described as the conidial state of Torrubiella albolanata Petch and later elevated to generic rank as a new genus, Granulomanus de Hoog & Samson (de Hoog 1978; Humber and Rombach 1987; Petch 1944). From the point of view of Humber and Rombach (1987), Granulomanus should be synonymized with Gibellula as it almost never occurs in the absence of Gibellula and/or its torrubiella-like sexual morph. Cylindrophora aranearum (≡Granulomanus aranearum (Petch) de Hoog & Samson) was henceforth synonymized with G. petchii. On the other hand, Samson and Evans (1992) argued that Granulomanus naturally occurs independently on spider hosts either with or without Gibellula. Thus, the genus should be retained as an independent asexually typified genus resulting in rejection of G. petchii. According to a recent taxonomic revision of the Cordycipitaceae, which was largely based on molecular data, several generic names including Granulomanus were suppressed (Kepler et al. 2017). Nevertheless, the taxonomic dilemma of G. petchii cannot yet be resolved owing to the lack of its sequence data. Based on these facts, only 17 species have been accepted in Gibellula (Shrestha et al. 2019).

Thailand is one of the most biodiverse countries in Southeast Asia and the BIOTEC culture collection has more than 700 Gibellula strains. Despite this number, only very few Gibellula species with distinct features could be recognized morphologically (Luangsa-ard et al. 2008, 2010). Gibellula gamsii is the most recently described species reported from Thailand (Kuephadungphan et al. 2019).

Our continuous survey of invertebrate-pathogenic fungi in Thailand for over two decades has led to the BIOTEC Bangkok Herbarium (BBH) and the BIOTEC Culture Collection (BCC) owning a very large herbaria, and culture collections, which greatly facilitates the exploration of existing species including Gibellula. Here, phylogeny within Gibellula species from the ribosomal internal transcribed spacer (ITS) regions analyzed prior to this study enabled recognition of four distinct clades. The morphological and multi-gene phylogenetic data confirm their identities as well as taxonomic placements. Herein, new species are described that are illustrated morphologically and phylogenetically and compared with other species in the same genus.

Materials and methods

Collection of fungal materials and isolation of pure cultures

Spiders parasitized by Gibellula spp. firmly attached on the underside of living leaves were collected from various locations throughout Thailand, mostly in the Northeastern region. The leaf bearing the parasitized spider was carefully detached from the tree, placed in a plastic box and transported to the laboratory for immediate isolation of a pure culture according to the protocols described by Kuephadungphan et al. (2014) and Mongkolsamrit et al. (2018). Briefly, the conidia located on the synnemata were gently swiped with small agar plugs of potato dextrose agar (PDA) which were then placed on a PDA plate. The conidia were allowed to germinate at 25 °C for a few days. Thereafter, each agar plug with actively growing mycelia was transferred to a fresh PDA plate where the fungus could readily grow for another 6–8 weeks. For specimens bearing sexual morphs, pure cultures were isolated by enabling ascospores from the perithecia to be discharged onto PDA plates and allowing them to grow at 25 °C for a certain amount of time depending on the growth rate of each individual strain. All cultures were required to be deposited in the BCC, Thailand while the fungal specimens were dried in an electric food dryer (50–55 °C) before being stored at the BBH, Thailand.

Morphological characterization

Microscopic characteristics were studied based on observation of synnemata and perithecia. Each of them was detached from the stroma and mounted on a microscope slide containing a drop of lactophenol cotton blue solution. Shapes and sizes of individual character were determined and measured according to Mongkolsamrit et al. (2018).

Identification of spider hosts

The mummified spiders were identified based on morphological characteristics. To better understand the host-pathogen relationship, posture of spider at attachment on leaf surface (touching or lifting), position of spider on the leaf (under or upper side), and leaf type (monocots or dicots) were herein recorded.

Molecular phylogenetic analyses

DNA extraction, PCR amplification of five DNA regions as well as purification of PCR products were conducted according to the protocols previously described by Kuephadungphan et al. (2019). The PCR amplicons were obtained using primers ITS1F (Gardes and Bruns 1993) and ITS4 (White et al. 1990) for nuc rDNA ITS1-5.8S-ITS2 (ITS barcode), LR5 or LR7 (Vilgalys and Hester 1990) and LROR (Bunyard et al. 1994) for the partial region of nuc 28SrDNA (LSU), 983F and 2218R (Rehner and Buckley 2005) for translation elongation factor 1-alpha (TEF1), RPB1-Ac and RPB1-Cr (Murata et al. 2014) for the largest subunit of RNA polymerase II (RPB1) and fRPB2-5F and fRPB2-7cR (Liu et al. 1999) for the second largest subunit of RNA polymerase II (RPB2).

DNA sequences were checked manually for ambiguous base calls and all sequences were assembled using BioEdit v.7.2.5 (Hall 1999; Hall et al. 2011). Sequence alignment was conducted using MAFFT 7.017 with G-INS as the algorithm and default settings used for gap opening and gap extension penalties (Katoh and Toh 2008). Manual adjustments were subsequently made in BioEdit. Concatenation of multiple loci was performed in GENEIOUS® 7.1.19 (http://www.geneious.com, Kearse et al. 2012).

Phylogenetic relationships were inferred using maximum likelihood (ML) with GTRCAT as the substitution model in RAXML 7.2.8 (Stamatakis 2006) and the rapid bootstrap analysis algorithm. Relative support for the branches was obtained from bootstrap analysis with 1,000 replicates. Bayesian analysis was performed according to Mongkolsamrit et al. (2019) using MRBAYES v.3.2.7 (Ronquist and Huelsenbeck 2003) on XSEDE via the online CIPRES Science gateway using SYM+G selected by MRMODELTEST 2.2 (Nylander 2004) as the best nucleotide substitution model. Posterior probabilities were performed by Markov Chain Monte Carlo Sampling (MCMC) in which four chains were run for 5,000,000 generations with a tree sampling frequency of 100 and a burn-in of 10% of the total run.

Results

Molecular phylogeny

The combined data set of 43 taxa (Table 1) comprised 4,325 characters including 680, 859, 917, 1,109 and 917 characters derived from ITS, LSU, TEF1, RPB1 and RPB2, respectively with Engyodontium aranearum as the outgroup. The multigene tree (Fig. 1) comprised seven different genera belonging to the family Cordycipitaceae including Akanthomyces, Beauveria, Blackwellomyces, Cordyceps, Engyodontium, Gibellula and Hevansia. The analyses showed the genera segregated corresponding to the recent phylogenetic classification of the Cordycipitaceae (Kepler et al. 2017; Kuephadungphan et al. 2019). The taxa of the new species were distributed in the Gibellula clade which was strongly supported (100%) and inferred as a monophyletic group. Gibellula pigmentosinum was found to be very close to Gibellula cf. alba by forming a strong supported clade together. Gibellula fusiformispora was inferred as the phylogenetic sister of G. cebrennini, whereas G scorpioiodes formed a distinct well-supported sister clade to these species.

Table 1.

List of taxa included in the phylogenetic analysis and their GenBank accession numbers. The isolates representing four new species and other sequences generated in this study are marked in bold.

Species Code GenBank accession numbers References
ITS LSU TEF1 RPB1 RPB2
Akanthomyces aculeatus HUA 772 KC519371 KC519370 KC519366 Sanjuan et al. 2014
A. aculeatus HUA 186145 MF416520 MF416465 Kepler et al. 2017
A. sabanensis ANDES-F 1014 KC633245 KC633248 KC875221 Chirivi-Salomon et al. 2015
A. sabanensis ANDES-F 1024T KC633232 KC875225 KC633266 KC633249 Chirivi-Salomon et al. 2015
Beauveria bassiana ARSEF 7518 HQ880762 HQ880975 HQ880834 HQ880906 Rehner et al. 2011
B. bassiana ARSEF 1564T NR111594 HQ880974 HQ880833 HQ880905 Rehner et al. 2011
Cordyceps militaris OSC 93623 JN049825 AY184966 DQ522332 DQ522377 AY545732 Kepler et al. 2012; Spatafora et al. 2007; Sung and Spatafora 2004
C. militaris ARSEF 5050 HQ880829 HQ881020 HQ880901 HQ880973 Rehner et al. 2011
C. farinosa CBS 111113T AY624181 MF416554 MF416499 MF416656 MF416450 Luangsa-ard et al. 2005; Kepler et al. 2017
C. javanica CBS 134.22T NR111172 NG059048 MF416504 MF416661 MF416455 Luangsa-ard et al. 2005; Kepler et al. 2017
C. javanica BCC 26304 MH532851 MH394660 MH521903 MH521825 MH521868 Helaly et al. 2019, this study
Blackwellomyces cardinalis OSC 93609T AY184962 DQ522325 DQ522370 DQ522422 Sung and Spatafora 2004; Spatafora et al. 2007
B. cardinalis OSC 93610 JN049843 AY184963 EF469059 EF469088 EF469106 Kepler et al. 2012; Sung and Spatafora 2004; Sung et al. 2007
Engyodontium aranearum CBS 309.85 JN036556 AF339526 DQ522341 DQ522387 DQ522439 Spatafora et al. 2007; Sung et al. 2001
E. aranearum CBS 658.80 LC092897 LC092916 Tsang et al. 2016
Hevansia novoguineensis CBS 610.80T MH532831 MH394646 MH521885 MH521844 Helaly et al. 2019; Mongkolsamrit et al. in press
H. novoguineensis NHJ 4314 EU369012 EU369051 EU369071 Johnson et al. 2009
H. novoguineensis NHJ 11923 EU369032 EU369013 EU369052 EU369072 Johnson et al. 2009
H. novoguineensis BCC 47881 JX192685 MH394650 MH521886 MH521807 MH521845 Helaly et al. 2017, this study
H. cinerea NHJ 3510 EU369009 EU369048 EU369070 Johnson et al. 2009
Gibellula cf. alba NHJ 11679 EU369016 EU369054 Johnson et al. 2009
G. cebrennini BCC 32072 MT477067 MT503326 This study
G. cebrennini BCC 39705 MH532874 MH394673 MH521895 MH521822 MH521859 This study
G. cebrennini BCC 53551 MT477068 MT503327 This study
G. cebrennini BCC 53605T MT477069 MT477062 MT503328 MT503321 MT503336 This study
G clavulifera var. alba ARSEF 1915T DQ518777 DQ522360 DQ522408 DQ522467 Spatafora et al. 2007
G. fusiformispora BCC 45076 MH532882 MH521823 MH521860 This study
G. fusiformispora BCC 56802T MT477070 MT477063 MT503329 MT503322 MT503337 This study
G. gamsii BCC 27968T MH152529 MH152539 MH152560 MH152547 Kuephadungphan et al. 2019
G. gamsii BCC 28797 MH152531 MH152541 MH152562 MH152549 MH152557 Kuephadungphan et al. 2019
G leiopus BCC 16025 MF416548 MF416492 MF416649 Kepler et al. 2017
G longispora NHJ 12014 EU369017 EU369055 EU369075 Johnson et al. 2009
G pulchra NHJ 10808 EU369035 EU369018 EU369056 EU369076 Johnson et al. 2009
G. pigmentosinum BCC 38246 MH532872 MH394672 MH521893 MH521800 MH521855 Helaly et al. 2019, this study
G. pigmentosinum BCC 39707 MH532875 MH394674 MH521894 MH521801 MH521856 Helaly et al. 2019, this study
G. pigmentosinum BCC 41203T MT477071 MT503330 MT503323 This study
G. pigmentosinum BCC 41870 MT477072 MT477064 MT503331 MT503324 This study
G. scorpioides BCC 13020 MT477073 MH394686 MH521901 MH521814 This study
G. scorpioides BCC 43298 MT477074 MH394677 MH521900 MH521816 MH521858 This study
G. scorpioides BCC 45127 MT477075 MT503332 This study
G. scorpioides BCC 47514 MT477076 MT503333 This study
G. scorpioides BCC 47530 MT477077 MT477065 MT503334 MT503338 This study
G. scorpioides BCC 47976T MT477078 MT477066 MT503335 MT503325 MT503339 This study
Figure 1. 

Phylogenetic tree inferred from a RAxML search of a concatenated alignment of ITS, LSU, TEF1, RPB1 and RPB2 showing the relationship among Gibellula and related genera. Bootstrap proportions/ Bayesian posterior probabilities ≥ 50% are provided above corresponding nodes; nodes with 100% support are shown as thick lines. The ex-type strains are marked with a superscript T (T) and the isolates reported in this study are bold.

Taxonomy

Gibellula cebrennini Tasanathai, Kuephadungphan & Luangsa-ard, sp. nov.

MycoBank No: 835113
Figure 2

Typification

Thailand, Nakhon Ratchasima, Khao Yai National Park, Mo Sing To Nature Trail; 14°711'N, 101°421'E; on Cebrenninus cf. magnus (Thomisidae, Araneae) attached to the underside of unidentified dicot leaf; 20 June 2012; K. Tasanathai, S. Mongkolsamrit, A. Khonsanit, W. Noisripoom, P. Srikitikulchai, K. Sansatchanon, R. Somnuk (Holotype no. BBH 35749, ex-type culture no. BCC 53604, isolated from ascospores and BCC 53605, isolated from conidia). GenBank (BCC 53605): ITS = MT477069, LSU = MT477062, TEF1 = MT503328, RPB1 = MT503321, RPB2 = MT503336.

Etymology

Refers to its spider host.

Description

Synnema arising from white to cream mycelial mat completely covering the spider host, cylindric, white to cream, slightly enlarged toward the sterile tip, consisting of multiseptate somewhat loosely bound longitudinal hyphae (Fig. 2a, c). Conidiophores scattered, arising from a network of hyphae loosely attached to the surface of the synnema, occasionally from a mycelium covering the host, (45–)95–139(–150) × (5–)5.5–7(–8) μm, verrucose, multiseptate, tapering abruptly to a short distinct neck, enlarging into a broadly ellipsoid to globose vesicle, (4.5–)5.5–7.5(–8.5) μm in diam (Fig. 2g). Metulae borne on vesicle, broadly obovoid to obovoid, (5–)6–7.5(–9) × (3–)4.5–6(–6.5) μm, bearing a group of narrowly obovoid phialides, thickened towards papillate apices, (4–)5.5–7.5(–9) × 1.5–2.5(–3.5) μm (Fig. 2h). Vesicle, metulae and phialides forming spherical heads, (23–)24–29.5(–33.5) μm in diam (Fig. 2h). Conidia fusiform, (4–)5.5–7.5(–9) × 1.5–2.5(–3.5) μm (Fig. 2i). Perithecia developed on subiculum of the host, arranged sparingly, occasionally crowded, superficial with a loose covering of cream mycelia, reddish yellow, ovoid, (1,150–)1,209–1,400(–1,411) × (375–)427–505(–575) μm (Fig, 2b, d). Asci over 600 μm long, (3.5–)4–5(–6) μm wide, ascus cap, (6–)7–8.5(–10) × (3.5–)4–4.5(–5) μm (Fig. 2e). Ascospores bacilliform, multiseptate, whole, over 570 μm long, 1–1.5 μm wide (Fig. 2f). Granulomanus-like asexual morph often occurring on the mycelial mat covering the host body. Polyblastic and irregularly shaped phialides developing multiple denticles, each bearing filiform conidium, (6–)7.5–10(–12) × 1–1.5 μm (Fig. 2j).

Figure 2. 

Gibellula cebrennini a fungus on spider (BBH 35749) b perithecia c a part of synnema showing conidiophores d perithecium e asci with apical apparatus f ascospores g conidiophore h conidial head i conidia j granulomanus-like asexual morph k colonies obverse and reverse on PDA at 25 °C after 28 days. Scale bars: 1 mm (d); 50 μm (e–f, g); 20 μm (h, j); 10 μm (i).

Culture characteristics

Colonies derived from conidia, on PDA slow-growing, attaining a diam of 1.4±0.05 cm in 4 weeks at 25 °C, white, velvety; reverse cream, becoming light brown with age toward center (Fig. 2k). Sporulation not observed.

Additional specimen examined

Thailand, Nakhon Ratchasima, Khao Yai National Park, Kong Kaeo Waterfall; 14°711'N, 101°421'E; on Araneida, underside of unidentified dicot leaf; 28 November 2006; K. Tasanathai, W. Chaygate, B. Thongnuch (BBH 18890, BCC 23863); Mo Sing To Nature Trail; 14°711'N, 101°421'E; on Cebrenninus cf. magnus, underside of unidentified dicot leaf; 18 June 2008; J. Luangsa-ard, K. Tasanathai, S. Mongkolsamrit, B. Thongnuch, P. Srikitikulchai, R. Ridkaew, W. Chaygate, R. Promharn (BBH 24673, BCC 32072). On Cebrenninus cf. magnus, underside of unidentified dicot leaf; 11 September 2009; K. Tasanathai, P. Srikitikulchai, S. Mongkolsamrit, T. Chohmee, R. Ridkaew (BBH 32685, BCC 39705 and BCC 39706). On Cebrenninus cf. magnus, underside of unidentified dicot leaf; 6 June 2012; K. Tasanathai, S. Mongkolsamrit, A. Khonsanit, W. Noisripoom, P. Srikitikulchai (BBH 32589, BCC 53551).

Gibellula fusiformispora Tasanathai, Kuephadungphan & Luangsa-ard, sp. nov.

MycoBank No: 835114
Figure 3

Typification

Thailand, Chiang Mai, Chiang Dao District, Ban Huathung; 19°420'N, 98°971'E; on Araneida attached to the underside of unidentified dicot leaf; 5 October 2012; K. Tasanathai, A. Khonsanit, W. Noisripoom, P. Srikitikulchai, R. Promharn (Holotype no. BBH 32918, ex-type culture no. BCC 56802, isolated from conidia) GenBank: ITS = MT477070, LSU = MT477063, TEF1 = MT503329, RPB1 = MT503322, RPB2 = MT503337.

Etymology

Refers to the fusiform part-spores.

Description

Spiders totally covered by the white to cream mycelial mat. A single synnema or synnemata in pairs cream to light brown, often darker than the mycelia covering the host, narrowing toward the apex and terminating in a swollen sterile tip with acute apex (Fig. 3a–c). Conidiophores arising laterally from the outer layer of synnemata, crowded, (23–)31–53(–83) × (4–)5.5–6.5(–7.5) μm, mostly verrucose, occasionally slightly roughed for very short conidiophores, abruptly narrowing to a slender apex and forming a globose to subglobose vesicle (Fig. 3c, i). Vesicles 6–7(–8) μm in diam, each bearing a number of metulae (Fig. 3j). Metulae obovoid to broadly obovoid, (7–)7.5–9(–10) × (4.5–)5–5.5(–6) μm (Fig. 3j). Phialides borne on metulae, narrowly obovoid, 7–8.5(–10) × 2–3 μm bearing fusiform to broadly fusiform conidia, (3.5–)4–5(–6) × 1.5–2(–2.5) μm (Fig. 3h, j). Conidial heads spherical, (31–)32–34.5(–37) μm in diam (Fig. 3j). Perithecia mostly appearing in pairs, ovoid, superficial with a loose covering of white to cream mycelia, reddish yellow, up to 1,000 μm in length, 320–350 μm in width (Fig. 3d–e). Asci 600–700 × 7–8 μm (Fig. 3f). Ascospores often disarticulating into part-spores. Part-spores fusiform, 12–15 × 2–3 μm (Fig. 3g). Granulomanus-like asexual morph absent.

Figure 3. 

Gibellula fusiformispora a fungus on a spider (BBH 38838) b fungus on a spider (BBH 32918) c synnemata (BBH 32918) d, e perithecia (BBH 38838) f ascus (BBH 38838) g ascospores (BBH 38838) h conidia (BBH 32918) i conidiophores showing conidial heads (BBH 32918) j conidial head bearing conidia (BCC 32918) k colonies obverse and reverse on PDA at 25 °C after 20 days. Scale bars: 250 μm (e); 100 μm (i); 50 μm (g); 20 μm (f, h, j).

Culture characteristics

Colonies derived from conidia, on PDA slow-growing, attaining a diam of 1.1±0.03 cm in 20 days at 25 °C, white, velvety; reverse cream, becoming light brown with age toward center (Fig. 3k). Sporulation not observed.

Additional specimen examined

Thailand, Chiang Mai, Chiang Dao District, Ban Huathung; 19°420'N, 98°971'E; on Deinopidae (Araneae) attached to the underside of unidentified monocot leaf; 23 September 2010; K. Tasanathai, P. Srikitikulchai, A. Khonsanit, K. Sansatchanon (BBH 38838, BCC 45076 and BCC 45077).

Notes

The sexual morph of G. fusiformispora is extremely close to Torrubiella ellipsoidea (Kobayasi and Shimizu 1982) in producing slightly curved fusiform part-spores of maximum 3 µm wide, whereas those of G. fusiformispora are almost two times wider. Considering the Gibellula conidial state, G. fusiformispora resembled G. cebrennini by forming synnema with sterile swollen tip, aspergillate conidiophores and fusiform conidia. However, G. fusiformispora can be easily recognized by having much shorter conidiophores, and the production of more than one synnema on the spider hosts.

Gibellula pigmentosinum Tasanathai, Kuephadungphan & Luangsa-ard, sp. nov.

MycoBank No: 835112
Figure 4

Typification

Thailand. Nakhon Ratchasima, Khao Yai National Park, Mo Sing To Nature Trail; 14°711'N, 101°421'E; on Storenomorpha sp., attached to underside of unidentified dicot leaf; 10 February 2010; K. Tasanathai, P. Srikitikulchai, S. Mongkolsamrit, T. Chohmee, R. Ridkaew, A. Khonsanit (Holotype no. BBH 28509, ex-type culture no. BCC 41203, isolated from ascospores and BCC 41204, isolated from conidia). GenBank (BCC 41203): ITS = MT477071, TEF1 = MT503330, RPB1 = MT503323.

Etymology

Refers to the capability of the fungus to produce pigmentosins.

Description

Spider host completely covered by white to yellowish-white mycelial mat. Synnemata solitary or in pairs, cylindrical, white, becoming yellowish-white at the base (Fig. 4a). Conidiophores arising along the entire length of the outer hyphae of synnemata and from the mycelia covering the host, crowded, smooth to verrucose, (55–)97.5–170(–226) × (5–)7–10(–12.5) µm, narrowing to a slender apex, and terminating in a swollen vesicle, metulae, phialides bearing conidia, forming a spherical conidial head (Fig. 4a, f). Conidial heads (25–)30–39(–45) µm diam (Fig. 4g). Vesicles mostly globose, (4.5–)5.5–9(–10) µm diam (Fig. 4g). Metulae borne on a vesicle, broadly obovoid, (5.5–)6–8(–10) × (3–)4–6(–7.5) µm (Fig. 4g), bearing phialides. Phialides obovoid to clavate, with a distinct short neck, (5–)5.5–8(–9) × 2–3(–4.5) µm (Fig. 4g). Conidia produced on a phialide, obovoid with an acute apex, (2.5–)3.5–5(–5.5) × 1–2(–3) µm (Fig. 4h). Perithecia produced on the mycelial mat on the head and body of the spider, scattered, superficial with loose mycelia covering only the bottom one-fourth of the perithecium, ovoid, reddish-yellow, (790–)882–1,117(–1,150) × 300–443(–475) μm (Fig. 4a, b). Asci cylindrical, 700–750 μm long, (4.5–)5–6(–7) μm wide, ascus cap (4–)5.5–6.5(–7) × 3.5–4(–5.5) μm (Fig. 4c). Ascospores filiform, multiseptate, arranged in parallel rows, (666–)670–727(–730) × 2–3 μm, often breaking into 128 part-spores (Fig. 4d). Part-spores bacilliform with apices rounded, (3.5–)4–7(–9) × 1–1.5(–3) μm (Fig. 4e). Granulomanus-like asexual morph occasionally present, forming irregularly branched hyphae bearing mono- or polyblastic phialides. Phialides irregularly in shape, mostly smooth, with one or more conspicuous denticles. A conidium borne on each denticle, long, filiform, (16–)16.5–21.5(–22.5) × 1–1.5 μm (Fig. 4i).

Figure 4. 

Gibellula pigmentosinum a fungus on spider (BBH 28509); b perithecia; c an ascus with an apical apparatus; d ascospore; e part-spores; f conidiophores; g conidial heads; h conidia; i granulomanus-like asexual morph; j colonies obverse and reverse on PDA at 25 °C after 28 days. Scale bars: 1 mm (b); 500 μm (d); 100 μm (f); 50 μm (g); 20 μm (c, i); 10 μm (e, h).

Culture characteristics

Colonies derived from ascospores, on PDA slow-growing, attaining a diam of 1.5±0.2 cm in 4 weeks at 25 °C, white, floccose; reverse light brown, darkening with age toward center (Fig. 4j). Sporulation not observed.

Additional specimens examined. Thailand, Nakhon Ratchasima, Khao Yai National Park, Mo Sing To Nature Trail; 14°711'N, 101°421'E; on Storenomorpha sp., underside of unidentified dicot leaf; 13 August 2009; K. Tasanathai, P. Srikitikulchai, S. Mongkolsamrit, T. Chohmee, R. Ridkaew (BBH 26516, BCC 38246 and BCC 38955); on Araneida, underside of unidentified dicot leaf; 11 September 2009; K. Tasanathai, P. Srikitikulchai, S. Mongkolsamrit, T. Chohmee, R. Ridkaew (BBH 27081, BCC 39707 and BCC 39708); on Storenomorpha sp., underside of unidentified dicot leaf; 7 April 2010; K. Tasanathai, S. Mongkolsamrit, T. Chohmee, A. Khonsanit, R. Ridkaew (BBH 28533, BCC 41870 and BCC 41871).

Notes

Gibellula pigmentosinum shares similarity with G pulchra (Mains 1950) in producing cylindric, yellowish white synnemata bearing aspergillate conidiophores with fusoid-ellipsoid conidia and superficial, reddish brown, ovoid perithecia containing bacilliform part-ascospores. The synnemata in G pulchra are more copious and sometimes more violaceous than in G. pigmentosinum. Remarkably, G. pigmentosinum distinctly differs from G pulchra in having a granulomanus-like conidial state.

Gibellula scorpioides Tasanathai, Khonsanit, Kuephadungphan & Luangsa-ard, sp. nov.

MycoBank No: 835115
Figure 5

Typification

Thailand, Nakhon Ratchasima, Khao Yai National Park, Mo Sing To Nature Trail, 14°711'N, 101°421'E; on Portia sp. attached to the underside of unidentified dicot leaf; 1 June 2011; K. Tasanathai, P. Srikitikulchai, S. Mongkolsamrit, A. Khonsanit, K. Sansatchanon, W. Noisripoom (Holotype no. BBH 31439, ex-type culture no. BCC 47975, isolated from ascospores and BCC 47976, isolated from conidia) GenBank (BCC 47976): ITS = MT477078, LSU = MT477066, TEF1 = MT503335, RPB1 = MT503325, RPB2 = MT503339.

Etymology

Refers to the outer appearance of the fungus resembling the posture of a scorpion.

Description

White to grayish- or brownish-white mycelial mat velvety, completely covering the spider host, firmly attaching the underside of living leaf by the mycelia covering its legs (Fig. 5a, b). Synnema solitary, arising from the posterior of the host abdomen, cylindrical, consisting of a compact bundle of parallel hyphae, 15–20 mm long with blunt tip. Conidiophores arising laterally from synnema, stout, smooth, mostly biverticillate, 20–29(–30) × 4 μm (Fig. 5d). Vesicles absent or hardly developed, bearing multiple metulae. Metulae obovoid, slightly broadening toward the base, (7–)9.5–12.5(–15) × (2–)3–5(–7) μm (Fig. 5e). A number of phialides borne on each metula, broadly cylindrical, abruptly tapering toward the apex, forming thickened distinct short neck, (9–)10–12.5(–14) × (2–)2.5–3.5(–4) μm, each bearing a conidium (Fig. 5e). Conidia fusiform, 5–7(–9) × (1.5–)2–3 μm (Fig. 5f). Sexual morph occasionally present. Perithecia occurring on the mycelial mat covering the host legs, occasionally on synnema particularly at base, superficial, mostly arranged in groups, ovoid, reddish yellow or light honey-brown, one-third immersed in the loose network of mycelia, 750–836(–870) × 310–361(–380) μm (Fig. 5c). Asci over 550 μm in length, (3–)4–5.5(–7) μm in width, ascus tip (4–)4.5–5 × 3–3.5(–4) μm (Fig. 5g, h). Ascospores often breaking into part-spores. Part-spores bacilliform, (9–)10–15(–22) × 1.5–2 μm (Fig. 5i). Granulomanus-like asexual morph absent.

Figure 5. 

Gibellula scorpioides a fungus on a spider (BBH 29669) b fungus on a spider (BBH 31439) c perithecia (BBH 31439) d conidiophores arising on synnema (BBH 29669) e penicillate conidiophore (BBH 29669) f conidia (BBH 29669) g asci (BBH 31439) h ascus with apical apparatus (BBH 31439) i ascospores (BBH 31439) j penicillate conidiophore produced on PDA k conidia on PDA l colonies obverse and reverse on PDA at 25 °C after 4 months. Scale bars: 500 μm (c); 50 μm (d, g); 20 μm (e, h–j); 10 μm (f, k).

Culture characteristics

Colonies derived from conidia, on PDA slow-growing, attaining a diam of 1.5±0.1 cm in 4 weeks at 25 °C, floccose, forming irregular margin, white, reverse cream, darkening toward center with age (Fig. 5l). Sporulation occurring after 3–4 months with the absence of synnema, forming a group of conidiophores, grey and scatter. Conidiophores biverticillate. Vesicles absent or hardly developed. Metulae obovoid, (10–)11–14.5(–16) × 3–5.5(–7) μm, each bearing cylindrical phialides, (10–)11.5–14(–16) × 3–4 μm. Conidia fusiform, 5–6(–7) × 3–3.5(–4) μm.

Additional specimens examined. Thailand, Chumphon, Phato District, Phato Watershed Conservation and Management Unit; 9°784'N, 98°699'E; on Portia sp., underside of unidentified dicot leaf; 10 March 2011; K. Tasanathai, P. Srikitikulchai, A. Khonsanit, K. Sansatchanon, D. Thanakitpipattana (BBH 30499, BCC 47530). Nakhon Ratchasima, Khao Yai National Park, Mo Sing To Nature Trail; 14°711'N, 101°421'E; on Portia sp., underside of unidentified dicot leaf; 1 June 2011; K. Tasanathai, P. Srikitikulchai, S. Mongkolsamrit, A. Khonsanit, K. Sansatchanon, W. Noisripoom (BBH 29669, BCC 43298).

Notes

The morphology of G. scorpioides appeared to be very close to G clavulifera var. clavulifera (Samson and Evans 1977), G clavulifera var. major (Tzean et al. 1997) and G clavulifera var. alba (Humber and Rombach 1987). The penicillate conidiophores were largely absent from the whip-like stroma in G clavulifera var. alba but distinctly present on a synnema of G. scorpioides. Based on a comparison of microscopic characteristics among G. scorpioides, varieties clavulifera, major and alba, the latter three were found to produce much longer conidiophores (up to 100 μm) than G. scorpioides (20–29(–30) × 4 μm) while the other characters such as metulae, phialides as well as conidia were considered to be not significantly different in both shape and size. Considering the presence of the torrubiella-like sexual morph, perithecia of G clavulifera var. alba were produced sparingly and separately on the host abdomen while those of G. scorpioides distinctly appeared in groups, only on the spider’s legs and basally on synnema. Nevertheless, an examination of additional specimens has led us to conclude that the sexual morph is not always present in G. scorpioides.

Discussion

A torrubiella-like sexual morph is well-known to be connected with Gibellula (Evans 2013; Kepler et al. 2017; Shrestha et al. 2019). Shrestha and colleagues (2019) recently reviewed spider-pathogenic fungi within Hypocreales including Gibellula where its sexual morph links are listed. Torrubiella globosa Kobayasi & Shimizu, Torrubiella globosostipitata Kobayasi & Shimizu, Torrubiella arachnophila var. pulchra Mains and Torrubiella gibellulae Petch were synonymized with G pulchra, species where their conidial and torrubiella-like sexual morphs often concurrently occur on the same substrates. Gibellula pigmentosinum appeared to be remarkably close to G pulchra in producing nearly identical microscopic characters in both shapes and sizes. Nonetheless, G. pigmentosinum distinctly differs from G pulchra in having a granulomanus-like conidial state. Considering its phylogenetic placement, G. pigmentosinum was significantly placed far from the taxon representing G pulchra which supported the morphological differences between them. Noticeably, G. pigmentosinum formed a very strongly supported clade together with Gibellula cf. alba. It is interesting that Gibellula cf. alba was not proposed as a species and it is unfortunate that the herbarium specimen of NHJ 11679 is no longer in a good condition. According to its placement in the RAxML/Bayesian tree inferred from multiple loci (Fig. 1), Gibellula cf. alba NHJ 11679 could unambiguously be assigned to G. pigmentosinum.

The morphological resemblance between G. cebrennini and G. fusiformispora as well as a multi-gene phylogenetic analysis indicate a very close relationship among these species. Moreover, they can be distinguished from each other by the length of conidiophores, the shape of ascospores as well as the presence of a granulomanus-like conidial state.

In nature, a torrubiella-like sexual morph may occur on spider hosts without the presence of Gibellula. It may be premature to assign the new species to Gibellula on the basis of sexual morph, when more than one asexually reproductive genus are known to be linked to a torrubiella-like sexual morph. Gibellula cebrennini and Akanthomyces thailandicus (Mongkolsamrit et al. 2018) are good examples of such a phenomenon. Based on an investigation of Thai specimens, G. cebrennini tended to produce torrubiella-like perithecia on the spider hosts in the absence of Gibellula and granulomanus-like asexual morphs, whereas A. thailandicus is an obligate parasite of spiders, of which only its torrubiella-like sexual morph has so far been recorded. These characteristics could lead to misidentification between these two species. The size and shape of part-spores are considered as the only morphological characters that have potential in species discrimination according to the evidence that G. cebrennini mostly produces longer bacilliform part-spores than A. thailandicus. In G. cebrennini and G. fusiformispora, it may also be difficult to discriminate them at first glance as the only distinguishing feature is the shape of their part-spores. These similiarities, and the occasional overlap in shape and size of morphological characters, were also demonstrated by Khonsanit et al. (2019) in the Ophiocordyceps irangiensis and O. myrmecophila species complex, by Mongkolsamrit et al. (2019) in Paraisaria phuwiangensis and P. yodhathaii, by Luangsa-ard et al. (2018) in Ophiocordyceps spp. with superficial perithecia, and Tasanathai et al. (2019) among termite pathogens in Ophiocordyceps. To improve species delimitation among closely related species with such very low morphological differentiation, integrative taxonomy combining a variety of data such as molecular, chemical, biogeographical, ecological characters, etc. is suggested to be very useful (Pante et al. 2015).

It has been over a half century since host specialization was suggested as one of the taxonomic criteria for parasitic fungi (Johnson 1968). In most cases of fungi that have a narrow host range or are restricted to a single host species, host specificity is considered as an important feature that can be used for identification at the species level (Vialle et al. 2013). In the case of invertebrate-pathogenic fungi, host specificity is usually taken into account mostly to evaluate their virulence and potential in terms of using them as biocontrol agents. For taxonomic purposes, Kobmoo and co-workers (2012) proved that host specificity has great potential for reflecting the divergent evolution of the ant-parasitic Ophiocordyceps unilateralis. Their success has therefore driven us to put effort for the first time to define the host species of Gibellula.

Bishop (1990), Hughes et al. (2016), and Savić et al. (2016) reported spider hosts of Gibellula distributed among 11 families consisting of Anyphaenidae, Agelinidae, Araneidae, Corinnidae, Linyphiidae, Pholcidae, Salticidae, Sparassidae, Theridiidae, Thomisidae, Zodariidae which represent approximately 10% of described families worldwide (World Spider Catalog 2020). Herein, three of those including Salticidae, Thomisidae and Zodariidae were found to be the hosts of G. scorpioides, G. cebrennini and G. pigmentosinum, respectively, whereas the family Deinopidae is reported here for the first time as a Gibellula host for G. fusiformispora.

According to the effort of putting toward species identification of spider hosts (Bristowe1930; Tikader 1965; Jocqué and Bosmans 1989; Deeleman-Reinhold 2001, 2009; Jocqué and Dippenaar-Schoeman 2007; Lehtinen et al. 2008; Benjamin 2011, 2016; Miller et al. 2012; Dhali et al. 2017; Jocqué et al. 2019), G. pigmentosinum, G. cebrennini as well as G. scorpioides were found to be exclusively specific to spider species with the exception of G. fusiformispora wherein only one specimen allowed us to identify the spider only to the family rank. Despite Gibellula being a well-known spider specialist, only Nentwig (1985), Hughes et al. (2016), and Savić et al. (2016) have ever indicated its hosts at genus or species ranks. In the current study, only the host of G. cebrennini could be identified to the species level as Cebrenninus cf. magnus, whereas hosts of G. pigmentosinum and G. scorpioides were assigned to Storenomorpha sp. (Zodariidae) and Portia sp. (Salticidae), respectively. One common problem is that the fungus tends to cover the host body completely, which can obscure the spider’s morphological features making identification infeasible. Tarsal claws and scopulae are important morphological features used to identify spider species (Wolff and Gorb 2012; Wolff et al. 2013; Labarque et al. 2017), and the morphology of spider feet was herein targeted and carefully studied. Since their legs appeared to be the only part that were slightly covered by fungal mycelia, it was thus considered to be the most significant character for distinguishing spider species infected by Gibellula. Host identification can be especially challenging for old fungal herbarium specimens that are dry and damaged. We suggest delivering specimens to arachnological taxonomists immediately after field work to allow the identification of spider hosts to species rank. Furthermore, as new species of spider continue to be described (e.g. Miller et al. 2012; Dhali et al. 2017; Jocqué et al. 2019), accurate taxonomy of spider hosts could be important for taxonomy of fungal pathogens on them.

It is notable that all seven host individuals used for identification in this study were found under a leaf. Portia sp. has a unique life history. These spiders are not only web invaders or cursorial hunters but are also web builders. Thus, they exist both on and off their own webs. Their webs are used for various activities including trapping, baiting, resting, molting, mating, oviposition, brooding (Jackson and Blest 1982; Jackson and Hallas 1986; Nelson and Jackson 2011a). They are day-active hunters and stay on their own webs at night (Barth 2002; Herland and Jackson 2004; Nelson and Jackson 2011b). Moreover, moribund web-building spiders infected by pathogenic fungi are presumed to stay motionless on their webs (Anotaux et al. 2016). This behaviour may promote growth and reproduction of G. scorpioides since spider silks possibly have antifungal properties (Tahir et al. 2015; Phartale et al. 2019). Another distinct feature of the spiders infected by G. scorpioides is its firm attachment to the substrate by only the mycelia growing over the tips of their legs allowing the host to sprawl and elevate their bodies upward. Interestingly, the host of G. scorpioides, Portia sp. is an araneophagic jumping spider that usually assumes such a posture during hunting (Forster and Murphy 1986). Furthermore, spiders are generally found dead with a posture of their legs flexed beneath their body (Pollard et al. 1987; Pizzi 2006; Starr and Taggart 2006). Such dead posture may also support growth and reproduction of G. scorpioides by keeping host from predation (Deeleman-Reinhold 2001; Honma et al. 2006), at least at the beginning stage of a fungal growth. From these observations, we believe that the fungus may influence the behaviour of the spider host by forcing it to stay firmly in place and assume an active posture during infection.

Since G. scorpioides can be cultured, it is possible to apply fungal spores to Portia spiders and study the spider-fungus interaction. It will be particularly interesting to investigate death sites, on or off web and death posture, resting or hunting postures between uninfected control and fungal infected spiders, which may give insight into behavioural manipulation before death by the fungus. Additionally, cultured G. scorpioides could be used to test the antifungal properties of Portia spider silk.

It is remarkable that our finding has revealed the high possibility to incorporate the host specificity in molecular and morphological criteria for classification and identification of Gibellula.

The biggest challenge for molecular phylogeny-based classification of Gibellula is the lack of reliable sequence data from type specimens. From sequences available in public databases, identities often appear erroneous, e.g. G. clavispora (Chen et al. 2016), G. curvispora (Han et al. 2013) and G shennongjiaensis (Zou et al. 2016) appeared to be closer to other ascomycetes than Gibellula based on ITS sequence data. In the past, no attempts were made to establish the described species as pure cultures, or the attempts failed, thus making molecular analysis impractical. The lack of sequence data from type strains of Gibellula makes it difficult to establish whether query sequences from new specimens represent new or rediscovered taxa.

Despite molecular phylogeny currently being the most powerful approach available in modern fungal classification and taxonomy (Ariyawansa et al. 2014), many attempts to incorporate alternative or polyphasic approaches, such as chemotaxonomy, have been made. This approach provides high-informative data to support morphological and molecular data for identifying fungal species, facilitates solving taxonomic problem as well as unraveling asexual morph-sexual morph links (Frisvad et al. 1990; Stadler et al. 2003; Helaly et al. 2018). As part of our ongoing research on taxonomy and secondary metabolite production of Thai invertebrate-pathogenic fungi, chemotaxonomy has been employed, resulting in the discovery of unprecedented secondary metabolites, including pigmentosin B from G. pigmentosinum and gibellamines from G. gamsii (Helaly et al. 2019; Kuephadungphan et al. 2019). These compounds were found to be species-specific and could be designated as chemotaxonomic markers for the species. However, it is premature to use such compounds as markers for Gibellula since the exploration of their secondary metabolite production is limited to only a few species. Chemotaxonomy must therefore be expanded to other taxa, in particular G. cebrennini, G. fusiformispora as well as G. scorpioides.

Acknowledgments

This work was supported by the National Center for Genetic Engineering and Biotechnology (BIOTEC) Platform Technology Management (Grant no. P19-50231), National Science and Technology Development Agency (NSTDA). This project has also received funding from the European Union’s Horizon 2020 Research and Innovation Staff Exchange (RISE) programme under the Marie Skłodowska-Curie grant agreement No 645701, Project acronym “GoMyTri”; lead beneficiaries JJL and MS. The National Park, Wildlife and Plant Conservation Department in Thailand is gratefully acknowledged for permission to conduct a study in the protected area. We are grateful to Dr. Philip James Shaw for the English editing and the reviewers whose comments and suggestions helped improve our manuscript.

References

  • Anotaux M, Toscani C, Leborgne R, Chaline N, Pasquet A (2016) Time till death affects spider mobility and web-building behavior during web construction in an orb-web spider. Current Zoology 62: 123–130. https://doi.org/10.1093/cz/zow001
  • Ariyawansa HA, Hawksworth DL, Hyde KD, Jones EG, Maharachchikumbura SS, Manamgoda DS, Thambugala KM, Udayanga D, Camporesi E, Daranagama A, Jayawardena R (2014) Epitypification and neotypification: guidelines with appropriate and inappropriate examples. Fungal Diversity 69: 5–91. https://doi.org/10.1007/s13225-014-0315-4
  • Barth FG (2002) Spider’s world: senses and behavior. Springer Verlag, Berlin, 394 pp.
  • Benjamin SP (2016) Revision of Cebrenninus Simon, 1887 with description of one new genus and six new species (Araneae: Thomisidae). Revue Suisse de Zoologie 123: 179–200.
  • Bishop L (1990) Entomophagous fungi as mortality agents of ballooning spiderlings. Journal of Arachnology 18: 237–238.
  • Cavara F (1894) Ulteriore contribuzione alla micologia lombarda. Atti dell’Ist Bot Univ e Lab Crittogamico Pavia 3: 313–350.
  • Chen WH, Han YF, Liang ZQ, Zou X (2016) Morphological traits, DELTA system, and molecular analysis for Gibellula clavispora sp. nov. from China. Mycotaxon 131: 111–121. https://doi.org/10.5248/131.111
  • Chirivi-Salomon JS, Danies G, Restrepo S, Sanjuan TI (2015) Lecanicillium sabanense sp. nov. (Cordycipitaceae) a new fungal entomopathogen of coccids. Phytotaxa 234: 63–74. https://doi.org/10.11646/phytotaxa.234.1.4
  • de Hoog GS (1978) Notes some fungicolous Hyphomycetes and their relatives. Persoonia 10: 33–81.
  • Deeleman-Reinhold CL (2001) Forest spiders of South East Asia: with a revision of the sac and ground spiders (Araneae: Clubionidae, Corinnidae, Liocranidae, Gnaphosidae, Prodidomidae and Trochanterriidae [sic]). Brill, Leiden, 591 pp.
  • Deeleman-Reinhold CL (2009) Spiny theridiids in the Asian tropics. Systematics, notes on behaviour and species richness (Araneae: Theridiidae: Chrysso, Meotipa). Contributions to Natural History 12: 403–436.
  • Dhali DC, Saha S, Raychaudhuri D (2017) Litter and ground dwelling spiders (Araneae: Arachnida) of reserve forests of Dooars, West Bengal. World Scientific News 63: 1–242.
  • Forster L, Murphy F (1986) Ecology and behaviour in Portia Schultzii, with notes on related Species (Araneae, Salticidae). The Journal of Arachnology 14: 29–42.
  • Hall T (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic acids symposium series 41: 95–98.
  • Hall T, Biosciences I, Carlsbad C (2011) BioEdit: an important software for molecular biology. GERF Bulletin of Bioscience 2: 60–61.
  • Han YF, Chen WH, Zou X, Liang ZQ (2013) Gibellula curvispora, a new species of Gibellula. Mycosystema 32: 777–780.
  • Helaly SE, Kuephadungphan W, Phainuphong P, Ibrahim MA, Tasanathai K, Mongkolsamrit S, Luangsa-ard JJ, Phongpaichit S, Rukachaisirikul V, Stadler M (2019) Pigmentosins from Gibellula sp. as antibiofilm agents and a new glycosylated asperfuran from Cordyceps javanica. Beilstein Journal of Organic Chemistry 15: 2968–2981. https://doi.org/10.3762/bjoc.15.293
  • Helaly SE, Kuephadungphan W, Phongpaichit S, Luangsa-ard JJ, Rukachaisirikul V, Stadler M (2017) Five unprecedented secondary metabolites from the spider parasitic fungus Akanthomyces novoguineensis. Molecules 22: 991–1001. https://doi.org/10.3390/molecules22060991
  • Helaly SE, Thongbai B, Stadler M (2018) Diversity of biologically active secondary metabolites from endophytic and saprotrophic fungi of the ascomycete order Xylariales. Natural Products Report 35: 992–1014. https://doi.org/10.1039/C8NP00010G
  • Herland DP, Jackson RR (2004) Portia perception: the umwelt of an araneophagic jumping spider. In: Prete FR (Ed.) Complex worlds from simpler nervous systems. London, England: MIT Press, 5–40.
  • Honma A, Oku S, Nishida T (2006) Adaptive significance of death feigning posture as a specialized inducible defence against gape-limited predators. Proceedings of the Royal Society B: Biological Sciences 273: 1631–1636. https://doi.org/10.1098/rspb.2006.3501
  • Hughes DP, Araújo J, Loreto R, Quevillon L, de Bekker C, Evans HC (2016) From So Simple a Beginning: The Evolution of Behavioral Manipulation by Fungi. In: Lovett B, St. Leger RJ (Eds) Genetics and molecular biology of entomopathogenic fungi (Advances in genetics), vol. 94. Cambridge: Academic Press, 1–33. https://doi.org/10.1016/bs.adgen.2016.01.004
  • Jackson RR, Blest AD (1982) The biology of Portia fimbriata, a web‐building jumping spider (Araneae, Salticidae) from Queensland: Utilization of webs and predatory versatility. Journal of Zoology 196: 255–293. https://doi.org/10.1111/j.1469-7998.1982.tb03504.x
  • Jackson RR, Hallas SEA (1986) Comparative biology of Portia africana, P. albimana, P. fimbriata, P. labiata, and P. shultzi, araneophagic, web-building jumping spiders (Araneae: Salticidae): Utilisation of webs, predatory versatility, and intraspecific interactions. New Zealand Journal of Zoology 13: 423–489. https://doi.org/10.1080/03014223.1986.10422978
  • Jocqué R, Bosmans R (1989) A revision of the genus Storenomorpha Simon (Araneae, Zodariidae). Spixiana 12: 125–134.
  • Jocqué R, Dippenaar-Schoeman AS (2007) Spider families of the world. Tervuren , Belgium: Royal Museum for Central Africa, 336 pp.
  • Jocqué R, Jocqué M, Stock W, Rin N, Henrard A (2019) The new Southeast Asian genus Cambonilla gen. nov. (Zodariidae, Araneae): ‘bisrepetitaplacent’. European Journal of Taxonomy 501: 1–24. https://doi.org/10.5852/ejt.2019.501
  • Johnson D, Sung GH, Hywel-Jones NL, Luangsa-ard JJ, Bischoff JF, Kepler RM, Spatafora JW (2009) Systematics and evolution of the genus Torrubiella (Hypocreales, Ascomycota). Mycological Research 113: 279–289. https://doi.org/10.1016/j.mycres.2008.09.008
  • Katoh K, Toh H (2008) Recent developments in the MAFFT multiple sequence alignment program. Briefings in Bioinformatics 9: 286–298. https://doi.org/10.1093/bib/bbn013
  • Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T (2012) Geneious Basic: an intergrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
  • Kepler RM, Luangsa-ard JJ, Hywel-Jones NL, Quandt C, Sung GH, Rehner SA, Aime MC, Henkel TW, Sanjuan T, Zare R, Chen M, Li Z, Rossman AY, Spatafora JW, Shrestha B (2017) A phylogenetically-based nomenclature for Cordycipitaceae (Hypocreales). IMA Fungus 8: 335–353. https://doi.org/10.5598/imafungus.2017.08.02.08
  • Kepler RM, Sung GH, Ban S, Nakagiri A, Chen M, Huang B, Li Z, Spatafora JW (2012) New teleomorph combinations in the entomopathogenic genus Metacordyceps. Mycologia 104: 182–197. https://doi.org/10.3852/11-070
  • Khonsanit A, Luangsa-ard JJ, Thanakitpipattana D, Kobmoo N, Piasai O (2019) Cryptic species within Ophiocordyceps myrmecophila complex on formicine ants from Thailand. Mycological progress 18: 147–161. https://doi.org/10.1007/s11557-018-1412-7
  • Kobayasi Y, Shimizu D (1982) Monograph of the genus Torrubiella. Bulletin of the National Science Museum (Tokyo) 8: 43–78.
  • Kobmoo N, Mongkolsamrit S, Tasanathai K, Thanakitpipattana D, Luangsa‐ard JJ (2012) Molecular phylogenies reveal host‐specific divergence of Ophiocordyceps unilateralis sensu lato following its host ants. Molecular Ecology 21: 3022–3031. https://doi.org/10.1111/j.1365-294X.2012.05574.x
  • Kuephadungphan W, Macabeo APG, Luangsa-ard JJ, Tasanathai K, Thanakitpipattana D, Phongpaichit S, Yuyama K, Stadler M (2019) Studies on the biologically active secondary metabolites of the new spider parasitic fungus Gibellula gamsii. Mycological Progress 18: 135–146. https://doi.org/10.1007/s11557-018-1431-4
  • Kuephadungphan W, Phongpaichit S, Luangsa-ard JJ, Rukachaisirikul V (2014) Antimicrobial activity of invertebrate-pathogenic fungi in the genera Akanthomyces and Gibellula. Mycoscience 55: 127–133. https://doi.org/10.1016/j.myc.2013.06.007
  • Labarque FM, Wolff JO, Michalik P, Griswold CE, Ramírez MJ (2017) The evolution and function of spider feet (Araneae: Arachnida): multiple acquisitions of distal articulations. Zoological Journal of the Linnean Society, 1–34. https://doi.org/10.1093/zoolinnean/zlw030
  • Lehtinen PT, Marusik YM (2008) A redefinition of Misumenops F. O. Pickard- Cambridge, 1900 (Araneae, Thomisidae) and review of the New World species. Bulletin of the British Arachnological Society 14: 173–198. https://doi.org/10.13156/100.014.0406
  • Luangsa-ard JJ, Tasanathai K, Mongkolsamrit S, Hywel-Jones NL (2008) Atlas of invertebrate-pathogenic fungi of Thailand volume 2. National Center of Genetic Engineering and Biotechnology, National Science and Technology Development: Thailand, 88 pp.
  • Luangsa-ard JJ, Tasanathai K, Mongkolsamrit S, Hywel-Jones NL (2010) Atlas of invertebrate-pathogenic fungi of Thailand volume 3. National Center of Genetic Engineering and Biotechnology, National Science and Technology Development: Thailand, 96 pp.
  • Luangsa-ard JJ, Tasanathai K, Thanakitpipattana D, Khonsanit A, Stadler M (2018) Novel and interesting Ophiocordyceps spp. (Ophiocordycipitaceae, Hypocreales) with superficial perithecia from Thailand. Studies in Mycology 89: 125–142. https://doi.org/10.1016/j.simyco.2018.02.001
  • Miller JA, Griswold CE, Scharff N, Rezáč M, Szűts T, Marhabaie M (2012) The velvet spiders: an atlas of the Eresidae (Arachnida, Araneae). Zookeys 195: 1–144. https://doi.org/10.3897/zookeys.195.2342
  • Mongkolsamrit S, Noisripoom W, Thanakitpipattana D, Wutikhun T, Spatafora JW, Luangsa-ard J (2018) Disentangling cryptic species with isaria-like morphs in Cordycipitaceae. Mycologia 110: 230–257. https://doi.org/10.1080/00275514.2018.1446651
  • Mongkolsamrit S, Noisripoom W, Arnamnart N, Lamlertthon S, Himaman W, Jangsantear P, Samson RA, Luangsa-ard JJ (2019) Resurrection of Paraisaria in the Ophiocordycipitaceae with three new species from Thailand. Mycological Progress 18: 1213–1230. https://doi.org/10.1007/s11557-019-01518-x
  • Mongkolsamrit S, Noisripoom W, Tasanathai K, Khonsanit A, Thanakitpipattana D, Himaman W, Kobmoo N, Luangsa-ard JJ (in press) Molecular phylogeny and morphology reveal cryptic species in Blackwellomyces and Cordyceps (Cordycipitaceae) from Thailand. Mycological Progress. https://doi.org/10.1007/s11557-020-01615-2
  • Murata N, Aoki T, Kusaba M, Tosa Y, Chuma I (2014) Various species of Pyricularia constitute a robust clade distinct from Magnaporthe salvinii and its relatives in Magnaporthaceae. Journal of General Plant Pathology 80: 66–72. https://doi.org/10.1007/s10327-013-0477-z
  • Nelson XJ, Jackson RR (2011a) Flexibility in the foraging strategies of spiders. In: Herberstein ME (Ed.) Spider behaviour: flexibility and versatility, Cambridge: Cambridge University Press, Cambridge, 31–56. https://doi.org/10.1017/CBO9780511974496.003
  • Nelson XJ, Jackson RR (2011b) Flexible use of anti-predator defences. In: Herberstein ME (Ed.) Spider behaviour: flexibility and versatility, Cambridge: Cambridge University Press, 99–126. https://doi.org/10.1017/CBO9780511974496.005
  • Nentwig W (1985) Parasitic fungi as a mortality factor of spiders. Journal of Arachnology 13: 272–274.
  • Nylander JAA (2004) MrModeltest v2. Progam distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden.
  • Petch T (1932) Gibellula. Annales Mycologici 30: 386–393.
  • Phartale NN, Kadam TA, Bhosale HJ, Karale MA, Garimella G (2019) Exploring the antimicrobial potential of Pardosa brevivulva silk. The Journal of Basic and Applied Zoology 80: 31. https://doi.org/10.1186/s41936-019-0102-6
  • Pollard SD, Macnab AM, Jackson RR (1987) Communication with chemicals: pheromones and spiders. In: Nentwig W (Ed.) Ecophysiology of spiders. Heidelberg, German: Springer Verlag, 133–141. https://doi.org/10.1007/978-3-642-71552-5_10
  • Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. https://doi.org/10.3852/mycologia.97.1.84
  • Rehner SA, Minnis AM, Sung GH, Luangsa-ard JJ, Devotto L, Humber RA (2011) Phylogeny and systematic of the anamorphic, entomopathogenic genus Beauveria. Mycologia 103: 1055–1073. https://doi.org/10.3852/10-302
  • Saccardo PA (1877) Fungi Italici. Michelia 1: 73–100.
  • Samson RA, Evans HC (1977) Notes on entomogenous fungi from Ghana IV. The genera Paecilomyces and Nomuraea. Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen: Series C 80: 128–134.
  • Sanjuan T, Tabima J, Restrepo S, Laessoe T, Spatafora JW, Franco-Malano AE (2014) Entomopathogens of Amazonian stick insects and locusts are members of the Beauveria species complex (Cordyceps sensu stricto). Mycologia 106: 260–275. https://doi.org/10.3852/13-020
  • Savić D, Grbić G, Bošković E, Hänggi A (2016) First records of fungi pathogenic on spiders for the Republic of Serbia Arachnologiche Mitteilungen/Arachnology Letters 52: 31–34. https://doi.org/10.5431/aramit5206
  • Shrestha B, Kubátová A, Tanaka E, Oh J, Yoon DH, Sung JM, Sung GH (2019) Spider-pathogenic fungi within Hypocreales (Ascomycota): their current nomenclature, diversity, and distribution. Mycological Progress 18: 983–1003. https://doi.org/10.1007/s11557-019-01512-3
  • Stadler M, Tichy H-V, Katsiou E, Hellwig V (2003) Chemotaxonomy of Pochonia and other conidial fungi with Verticillium-like anamorphs. Mycological Progress 2: 95–122. https://doi.org/10.1007/s11557-006-0048-1
  • Starr C, Taggart R (2006) Biology: the unity and diversity of life. Belmont, California: Wadsworth Publishing Company, 1056 pp.
  • Sung GH, Hywel-Jones NL, Sung JM, Luangsa-ard JJ, Shrestha B, Spatafora JW (2007) Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Study Mycology 57: 5–59. https://doi.org/10.3114/sim.2007.57.01
  • Sung GH, Spatafora JW, Zare R, Hodge KT, Gams W (2001) A revision of Verticillium sect. Prostrata. II. Phylogenetic analyses of SSU and LSU nuclear rDNA sequences from anamorphs and teleomorphs of the Clavicipitaceae. Nova Hedwigia 72: 311–328.
  • Tahir HM, Zaheer A, Yaqoob R (2015) Antifungal potential of silk recovered from Neoscona theisi (Araneae: Araneidae). Punjab University Journal of Zoology 30: 81–83.
  • Tasanathai K, Noisripoom W, Chaitika T, Khonsanit A, Hasin S, Luangsa-ard J (2019) Phylogenetic and morphological classification of Ophiocordyceps species on termites from Thailand. MycoKeys 56: 101–129. https://doi.org/10.3897/mycokeys.56.37636
  • Tikader BK (1965) On some new species of spiders of the family Thomisidae from India. Proceedings of the Indian Academy of Science 61: 277–289.
  • Tsang CC, Chan JF, Pong WM, Chen JH, Ngan AH, Cheung M, Lai CK, Tsang DN, Lau SK, Woo PC (2016) Cutaneous hyalohyphomycosis due to Parengyodontium album gen. et. comb. nov. Medical Mycology 54: 699–713. https://doi.org/10.1093/mmy/myw025
  • Vialle A, Feau N, Frey P, Bernier L, Hamelin RC (2013) Phylogenetic species recognition reveals host-specific lineages among poplar rust fungi. Molecular Phylogenetics and Evolution 66: 628–644. https://doi.org/10.1016/j.ympev.2012.10.021
  • Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/JB.172.8.4238-4246.1990
  • White TJ, Bruns TD, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds) PCR protocols: a guide to methods and applications. San Diego: Academic Press, 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  • World Spider Catalog (2020) World spider catalog. Version 21.0 Natural History Museum Bern, available online at http://wsc.nmbe.ch/
  • Zou X, Chen WH, Han YF, Liang ZQ (2016) A new species of the genus Gibellula. Mycosystema 35: 1161–1168.