Research Article |
Corresponding author: Ting-Chi Wen ( tingchiwen@yahoo.com ) Academic editor: Marc Stadler
© 2019 Yuan-Pin Xiao, Sinang Hongsanan, Kevin D. Hyde, Siraprapa Brooks, Ning Xie, Feng-Yao Long, Ting-Chi Wen.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Xiao Y-P, Hongsanan S , Hyde KD, Brooks S, Xie N, Long F-Y, Wen T-C (2019) Two new entomopathogenic species of Ophiocordyceps in Thailand. MycoKeys 47: 53-74. https://doi.org/10.3897/mycokeys.47.29898
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Ophiocordyceps is entomopathogenic and the largest studied genus in the family Ophiocordycipitaceae. Many species in this genus have been reported from Thailand. The first new species introduced in this paper, Ophiocordyceps globiceps, differs from other species based on its smaller perithecia, shorter asci and secondary ascospores and additionally, in parasitising fly species. Phylogenetic analyses of combined LSU, SSU, ITS, TEF1α and RPB1 sequence data indicate that O. globiceps forms a distinct lineage within the genus Ophiocordyceps as a new species. The second new species, Ophiocordyceps sporangifera, is distinguished from closely related species by infecting larvae of insects (Coleoptera, Elateridae) and by producing white to brown sporangia, longer secondary synnemata and shorter primary and secondary phialides. We introduce O. sporangifera based on its significant morphological differences from other similar species, even though phylogenetic distinction is not well-supported.
2 new taxa, Hypocreales , morphology, phylogenetic, taxonomy
The genus Ophiocordyceps was introduced by
Thailand is located in the tropical areas with a rich biodiversity (
Specimens were collected in The Mushroom Research Centre, Chiang Mai, Thailand, from soil and grass litter and taken to the laboratory. Fruiting bodies were examined using free hand sections under a stereomicroscope. Water-mounted slides were prepared for a microscope study and photographed under a compound microscope. Strains were isolated from single spores by using the protocol in
DNA was extracted from both dried specimens and cultures by using E.Z.N.A.TM Fungal DNA MiniKit (Omega Biotech, CA, USA), according to the manufacturers protocols. Universal known primers were used in PCR amplification; ITS4/ITS5 for internal transcribed spacer gene region (ITS), NS1/NS4 for partial small subunit ribosomal RNA gene region (SSU), LROR/LR5 for partial large subunit rDNA gene region (LSU) (
Sequence data were obtained from GenBank based on previous studies as listed in Table
Species | Insecta | Voucher | SSU | ITS | LSU | TEF1α | RPB1 | References |
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H. dipterigena | Diptera | NHJ12170.02 | GU723771 | GU797126 |
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O. acicularis | Coleoptera (larva) | OSC 110988 | EF468951 | EF468804 | EF468745 | EF468853 |
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O. agriotidis | Coleoptera (larva) | ARSEF 5692 | DQ522540 | JN049819 | DQ518754 | DQ522322 | DQ522368 |
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O. amazonica | Orthoptera (Acrididae imago) | Ophama2026 | KJ917562 | KJ917571 | KM411989 | KP212902 |
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O. annulata | Coleoptera | CEM 303 | KJ878915 | KJ878881 | KJ878962 | KJ878995 |
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O. aphodii | Coleoptera | ARSEF 5498 | DQ522541 | DQ518755 | DQ522323 |
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O. appendiculata | Coleoptera (larva) | NBRC 106960 | JN941728 | JN943326 | JN941413 | AB968577 | JN992462 |
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O. arborescens | Cossida (larva) | NBRC 105891 | AB968398 | AB968414 | AB968572 |
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O. australis | Hymenoptera (ant) | Ophaus992 | KC610785 | KC610766 | KC610731 | KF658663 |
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O. barnesii | Coleoptera (larva) | BCC28560 | EU408776 | EU408773 |
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O. brunneinigra | Hemiptera (Cicadellidae) | TBRC 8093 | MF614654 | MF614638 | MF614668 |
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O. brunneiperitheciata | Lepidoptera (larva) | TBRC 8100 | MF614658 | MF614643 |
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O. brunneipunctata | Coleoptera (Elateridae larva) | OSC 128576 | DQ522542 | DQ518756 | DQ522324 | DQ522369 |
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O. buquetii | Hymenoptera (Formicidae) | HMAS 199613 | KJ878939 | KJ878904 | KJ878984 | KJ879019 |
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O. citrina | Hemiptera | TNS F18537 | KJ878903 | KJ878983 |
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O. clavata | Coleoptera (larva) | NBRC 106962 | JN941726 | JN943328 | JN941415 | AB968587 | JN992460 |
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O. coccidiicola | Insect | NBRC 100682 | AB968404 | AB968419 | AB968583 |
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O. coccidiicola | Insect | HMAS199612 | KJ878917 | AB027377 | KJ878884 | KJ878965 | KJ878998 |
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O. coenomyia | Coenomyia (larva) | NBRC 108993 | AB968384 | AB968396 | AB968412 | AB968570 |
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O. communis | Coleoptera | NHJ 12581 | EF468973 | EF468831 | EF468775 |
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O. cossidarum | Lepidoptera (larva) | MFLU 17-0752 | MF398186 | MF398187 | MF928403 | MF928404 |
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O. crinalis | Lepidopteran (larva) | HIMGD17327 | EU149926 |
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O. curculionum | Coleoptera (adult Curculionidae) | OSC 151910 | KJ878918 | KJ878885 | KJ878999 |
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O. cylindrospora | Hymenoptera (adult wasp) | MFLU: 17-1961 | MG553651 | MG553635 | MG553652 |
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O. dipterigena | Diptera (adult fly) | MY621 | GU723764 | GU797126 |
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O. dipterigena | Diptera (adult fly) | MRCIF71 | EU573346 |
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O. dipterigena | Diptera (adult fly) | OSC 151912 | KJ878920 | KJ878887 | KJ878967 | KJ879001 |
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O. elongata | Lepidoptera (larva) | OSC 110989 | EF468808 | EF468748 | EF468856 |
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O. emeiensis | Lepidoptera (larva) | G96031 | AJ309347 |
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O. entomorrhiza | Lepidoptera | KEW 53484 | EF468954 | JN049850 | EF468809 | EF468749 | EF468857 |
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O. evansii | Hymenoptera (Pachycondylaharpax) | Ophsp 858 | KC610796 | KC610770 | KC610736 | KP212916 |
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O. forquignonii | Diptera (adult fly) | OSC 151908 | KJ878922 | KJ878889 | KJ879003 |
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O. formicarum | Camponotus (Ant) | BCMU CF 01 | AB222678 |
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O. formicarum | Camponotus (Ant) | BCMU CF 02 | AB222679 |
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O. formosana | Coleoptera (larva) | MFLU: 15-3888 |
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O. fulgoromorphila | Hemiptera (Fulgoridae adult) | Ophara717 | KC610794 | KC610760 | KC610729 | KF658676 |
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O. geometridicola | Lepidoptera (Geometridae) | TBRC 8095 | MF614648 | MF614632 | MF614663 |
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O. globiceps | Diptera (adult fly) | MFLUCC 18-0495 | MH725811 | MH725815 | MH725829 | MH727387 | This study | |
O. globiceps | Diptera (adult fly) | MFLU 18-0661 | MH725812 | NH725816 | MH725830 | MH727388 | This study | |
O. gracilis | Lepidoptera (larva) | EFCC 8572 | EF468956 | JN049851 | EF468811 | EF468751 | EF468859 |
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O. hemisphaerica | Diptera (adult fly) | FLOR 59525 | KX197233 |
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O. heteropoda | Hemiptera (cicada nymph) | OSC 106404 | AY489690 | AY489722 | AY489617 | AY489651 |
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O. irangiensis | Hymenoptera (adult ant) | OSC 128579 | EF469123 | EF469076 | EF469060 | EF469089 |
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O. issidarum | Hemiptera (adult) | MFLU:17-0751 | MF398185 | MF398188 |
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O. karstii | Hepialus (larva) | MFLU:15-3884 | KU854952 | KU854945 | KU854943 |
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O. konnoana | Coleoptera (larva) | EFCC 7315 | EF468959 | EF468753 | EF468861 |
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O. lanpingensis | Hepialus (larva) | YHOS0707 | KC417459 | KC417461 | KC417463 | KC417465 |
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O. lloydii | Hymenoptera (Camponotus) | OSC 151913 | KJ878924 | KJ878891 | KJ878970 | KJ879004 |
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O. longissima | Hemiptera (cicada nymph) | NBRC 108989 | AB968394 | AB968407 | AB968421 | AB968585 |
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O. macroacicularis | lepidopterans (larvae) | NBRC 105888 | AB968389 | AB968401 | AB968417 | AB968575 |
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O. melolonthae | Coleoptera (Scarabeidae larva) | OSC 110993 | DQ522548 | DQ518762 | DQ522331 | DQ522376 |
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O. multiperitheciata | Lepidoptera (larva) | BCC 69008 | MF614657 | MF614641 |
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O. myrmecophila | Hymenoptera (adult ant) | MFLU 16-2912 | MF351730 | MF351726 | MF372585 | MF372759 |
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O. myrmicarum | Formicidae (adult ant) | ARSEF11864 | KJ680150 | JX566973 | KJ680151 |
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O. neovolkiana | Coleoptera | OSC 151903 | KJ878930 | KJ878896 | KJ878976 | KJ879010 |
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O. nigra | Hemiptera | TNS 16252 | KJ878941 | KJ878906 | KJ878986 |
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O. nigrella | Lepidoptera (larva) | EFCC 9247 | EF468963 | JN049853 | EF468818 | EF468758 | EF468866 |
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O. nutans | Hemiptera (Pentatomidae adult) | OSC 110994 | DQ522549 | DQ518763 | DQ522333 | DQ522378 |
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O. odonatae | Odonata (Dragonfly) | TNS F18563 | D86055 | AB104725 |
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O. pauciovoperitheciata | Lepidoptera (larva) | TBRC 8106 | MF614652 | MF614633 |
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O. pseudoacicularis | Lepidoptera (larva) | TBRC 8102 | MF614646 | MF614630 | MF614661 |
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O. pulvinata | Hymenoptera (adult ant) | TNS-F 30044 | GU904208 | GU904209 | GU904210 |
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O. purpureostromata | Coleoptera | TNS F18430 | KJ878931 | KJ878897 | KJ878977 | KJ879011 |
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O. pseudolloydii | Formicidae (adult ant) | MFLU 15-1425 | MF351725 | MF372758 | MF372761 |
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O. ramosissimum | Lepidoptera (larva) | GZUHHN8 | KJ028012 | KJ028007 | KJ028014 | KJ028017 |
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O. ravenelii | Coleoptera (larva) | OSC 110995 | DQ522550 | DQ518764 | DQ522334 | DQ522379 |
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O. rhizoidea | Isoptera (adult termite) | NHJ 12529 | EF468969 | EF468824 | EF468765 | EF468872 |
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O. robertsii | Lepidoptera (Hepialidae larva) | KEW 27083 | EF468826 | EF468766 |
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O. rubiginosiperitheciata | Coleoptera (larva) | NBRC 106966 | JN941704 | JN943344 | JN941437 | AB968582 | JN992438 |
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O. sinensis | Lepidopteran pupa | EFCC7287 | EF468971 | JN049854 | F468767 | EF468874 |
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O. sobolifera | Hemiptera (cicada nymph) | NBRC 106967 | AB968395 | AB968409 | AB968422 | AB968590 |
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O. sp | FMF147 | KX197238 |
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O. sp | OSC 110997 | EF468976 | EF468774 | EF468879 |
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O. spataforae | Hemiptera (Fulgoridae) | NHJ 12525 | EF469125 | EF469078 | EF469063 | EF469092 |
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O. sphecocephala | Hymenoptera (adult wasp) | NBRC 101753 | JN941695 | JN943350 | JN941446 | AB968592 | JN992429 |
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O. sporangifera | Lepidoptera (Cossidae) | MFLUCC 18-0492 | MH725814 | MH725818 | MH725832 | MH727390 | MH727392 | This study |
O. sporangifera | Lepidoptera (Cossidae) | MFLU 18-0658 | MH725813 | MH725817 | MH725831 | MH727389 | MH727391 | This study |
O. stylophora | Coleoptera (Elateridae larva) | OSC 111000 | DQ522552 | JN049828 | DQ518766 | DQ522337 | DQ522382 |
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O. superficialis | Insect | MICH 36253 | EF468983 | EF468883 |
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O. thanathonensis | Hymenotera (adult ant) | MFU 16-29010 | MF882926 | MF850375 | MF850375 | MF872614 | MF872616 |
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O. tricentri | Hemiptera (Cercopidae) | NBRC 106968 | AB968393 | AB968410 | AB968423 | AB968593 |
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O. unilateralis | Hymenoptera (Camponotus) | OSC 128574 | DQ522554 | DQ518768 | DQ522339 | DQ522385 |
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O. variabilis | Diptera (larva) | OSC 111003 | EF468985 | EF468839 | EF468779 | EF468885 |
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O. xuefengensis | Lepidoptera (Hepialidae larva) | GZUH2012HN19 | KC631788 | KC631803 | KC631794 | KC631799 |
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O. yakusimensis | Hemiptera (cicada nymph) | HMAS 199604 | KJ878938 | KJ878902 | KJ879018 |
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T. inflatum | Coleoptera (larva) | OSC 71235 | EF469124 | JN049844 | EF469077 | EF469061 | EF469090 |
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T. ophioglossoides | Fungi (Elaphomyces sp.) | NBRC 106332 | JN941732 | JN943322 | JN941409 | JN992466 |
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Maximum likelihood trees (ML) were estimated by using the software RAxML 7.2.8 Black Box (
Phylogram of Ophiocordyceps globiceps and O. sporangifera generated from maximum likelihood (RAxML) analysis of ITS, SSU, LSU, RPB1 and TEF1α sequence data. Tolypocladium inflatum and T. ophioglossoides were used as outgroup taxon. Maximum likelihood bootstrap values greater than 75% and Bayesian posterior probabilities over 0.90 were indicated above the nodes. The new species are indicated in red.
Eighty-seven taxa (including the four with new sequence data) were included in the combined ITS, SSU, LSU, RPB1 and TEF1α dataset (Table
The specific epithet refers to the feature of the secondary hemispherical to globoid fertile head.
Sexual morph: Stromata 4–8 mm long × 0.5–1 mm diam., one or several from the host, stipitate, capitate, unbranched, cinnamon to yellow. Stipe 3.5–7.5 mm long, 0.2–0.5 mm diam., yellow, cylindrical, with a fertile apex. Fertile head 1–1.5 mm long, 1–1.2 mm diam., cinnamon to yellow, single, hemispherical to globoid. Perithecia 538–663 × 182–247 μm (x̄= 600 × 214 µm, n = 60), immersed, ovoid to elongated pyriform, thick-walled, vertical with the ostioles opening on the upper surface of the head. Peridium 17–22 µm (x̄ = 20 µm, n = 90) wide, hyaline, of textura porrecta to textura prismatica to textura angularis. Asci 373–454 × 5.7–8.2 μm (x̄ = 413 × 7 µm, n = 90), 8-spored, hyaline, filiform, with a thick apex. Apical cap 4.4–6.4 × 4.9–5.7 μm (x̄ = 5.4 × 5.3 µm, n = 60), thick, with a small channel in the centre. Ascospores 240–303 × 1.8–2.3 μm (x̄ = 272 × 2.1 µm, n = 60), filiform, hyaline, multiseptate. Secondary ascospores 4–5.4 × 1.2–1.9 μm (x̄ = 4.7 × 1.6 µm, n = 90) cylindrical to fusoid, 1-celled, straight, hyaline, smooth-walled. Asexual morph: Undetermined.
Ophiocordyceps globiceps (holotype MFLU 18–0661). a Habitat b Ascostroma emerging from infected fly c Host d Fertile head of ascostroma e Vertical section of the stroma f Section of ascomata g Peridium h, i Asci k Apical cap of asci l, q Part of ascospore m, n Secondary ascospores o Upper side of the culture p Reverse side of the culture. Scale bars: 1000 µm (b–d), 500 µm (e, f), 100 µm (h, i), 20 µm (g), 10 µm (k, l), 5 µm (m, n, q), 5 cm (o, p).
growing on PDA, reaching 5 cm diam., after 6 weeks at 25 °C, superficial cottony, whitened, loose, reverse yellow. After 10 weeks at 25 °C, reaching 6 cm diam., no conidiogenous structures observed.
THAILAND, Ranong, Tambon Khao Niwet, parasitise on fly (Muscidae, Diptera) 7 mm long, 3 mm wide, brown to dark brown, without hyphae on the surface, collected on the grass stem, 19 July 2015, YuanPin Xiao, (MFLU 18–0661, holotype, ex-type living culture, MFLUCC 18–0495); Chiang Mai, Thailand, on adult fly (Diptera), 6.5 mm long, 2.7 mm wide, brown to dark brown, without hyphae on the surface, collected on the grass, 19 July 2017, YuanPin Xiao, (MFLU 18–0662, paratypes, living culture MFLUCC 18–0496).
In the phylogenetic tree, Ophiocordyceps globiceps is closely related to O. dipterigena (Berk. & Broome) G.H. Sung, J.M. Sung, Hywel-Jones & Spatafor. (Thailand) and O. hemisphaerica Mafalda-Freire, Reck & Drechsler-Santos (Brazil), which infect flies (
We compared the new species with other Ophiocordyceps species which infect flies (Diptera) or are morphologically similar to O. globiceps (Table
Species | Location | Host | Stromata (mm) | Stipe (mm) | Fertile part (mm) | Perithecia (μm) | Asci (μm) | Ascospores (μm) | Part-spores (μm) | Reference |
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C. sakishimensis | Japan | Diptera | 6–7 long, cylindrical, white | 500 × 250–260, superficial, ovoid | 4–6 × 1, cylindrical |
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O. dipterigena (First record) | Sri Lanka | 5–10 × 1, pale | Cylindrical | Globose | 10 × 1.5 |
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O. dipterigena | Japan | Diptera | 5–8 long, 1–2 wide, 0.5–1 wide, orange-cinnamon or cinnamon-brown | 0.2–0.5 thick, orange-cinnamon to light yellow | Narrowly ovoid or conoid, 700–900 × 240–400, wall 15–25 thick | 480–600 long | Filiform, multiseptate | 6–12 × 1–1.5, cylindric or fusoid fragments |
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O. dipterigena | Thailand | Diptera | 4–10 long, pale cream-yellow to orange-brown | 1–1.5 high, 1.5–2.5 diam., terminal, disc-like to subglobose | 800–1000 × 200–300, narrowly ovoid to obclavate | 450–600 × 4–6, cylindrical | Filiform, breaking up into 64 part-spore | 6–12 × 1–1.5, cylindrical to fusiform |
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O. discoideicapitata | Japan | Diptera | 2.5–3.5 × 0.7–1.2, two | 3–4, discoid, laterally conical | 620–700 × 200–250, pyriform | 5–6 diam., filiform | 6–9 × 1, cylindrical, truncated |
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O. forquignonii | Diptera | 3-6 long, subfiliform, with a cylindrical apex | Cylindrical | Ellipsoid | Oval, 8 |
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O. globiceps | Thailand | Diptera | 4–8 long × 0.5–1 diam., unbranched, cinnamon to yellow, one or several from host | 3.5–7.5 long, 0.2–0.5 diam., cinnamon to yellow, cylindrical, with a fertile apex | 1–1.5 long, 1–1.2 diam., yellow, hemispherical to globoid | 538–663 × 182–247, ovoid to elongated pyriform | 373–454 × 5.7–8 | 240–303 × 1.8–2.3, filiform, hyaline, multiseptate | 4–5.4 × 1.2–1.9, cylindrical to fusoid | This study |
O. hemisphaerica | Brazil | Diptera (Muscidae) | 12–20 × 0.8–1, unbranched, brown to greyish-brown | 11–19 long, 0.8–1 wide, cylindrical, with a fertile apex | 1–1.2 long, 2–4 diam., hemispherical | 780–860 × 220–290, Obpyriform, slightly curved | 500–640 × 5–6 | Filiform, more than 52 septa | 7–10 × 1–1.5, cylindrical to unusually fusoid |
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O. lacrimoidis | Brazil | Diptera | 4–5 × 1, two, simple | 3–4 long, 1 wide, cylindrical, epidermal layer brown, medullar region white to cream | 1.2 long, 1.8–2.2 diam., discoid, pale to dark yellowish | 650–700 × 200–250, immersed, obpyriform, slightly curved | 350–450 × 5, narrow cylindrical | Filiform, as long as asci, hyaline, more than 56 septa | 8–14 × 2, cylindrical, hyaline |
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O. muscicola = C. muscicola | Brazil | Diptera | 9–13 × 0.5–1, two to six, rarely branched | 2–4 × 1–1.2, discoid | 850–920 × 230–300, pyriform | 550–700 × 5, filiform | 650–700 × 2, 64 part-spores | 11–14 × 2, terminal cylindrical, intermediates fusoids 8–10 × 1–2 |
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The specific epithet refers to the feature of the sporangium-bearing.
Sexual morph: Unknown. Asexual morph: Primary synnema 9–18 cm high 1–2 mm diam., arising from the head region of the larva, branching into 2–5, cylindrical, brown to deep brown, with small white fertile head on the top, not smooth. Fertile head 500–2000 µm long, 400–1000 µm diam., globose to subglobose, capitulum, white to brown, arising from the apical end of primary synnema, mess of sporangium on the surface. Sporangium 78–121 µm diam. (x̄ = 100 µm, n = 60), spherical, arising from the apical end of primary synnema, white colour when immature, becoming brown to dark brown after maturity, consisting of thick-walled cells. Secondary synnemata 1092–1937 × 21–34 µm, (x ‒= 1515 × 27 µm, n = 60), laterally from the primary synnema, brown to white, cylindrical, not smooth. Hyphae 1.8–2.8 µm wide (x̄ = 2.3 µm, n = 60), irregularly multi-septate, brown, cylindrical, smooth or rough, sometimes particularly expand. Phialides 25–40 × 1.3–2.5 µm (x̄ = 33 × 1.9 µm, n = 60), hirsutella-like, hyaline, solitary, unbranched, narrow slender, smooth. Conidia 6.7–9.8 × 2.5–3.8 µm (x̄ = 8.3 × 3.2 µm, n = 60), 1 cell, hyaline, subglobose to reniform, bound in mucilaginous spheres. Mucilaginous spheres 10.5–12.9 × 6.4–8.7 µm (x̄ = 11.7 × 7.5 µm, n = 60), composed of 1–12 conidia, hyaline, at phialide apex.
Ophiocordyceps sporangifera (holotype MFLU 18–0658). a Habitat b Synnemata on host surface c Host d, e Synnemata f Fertile head of primary synnema g Sporangium h Secondary synnema i Sporangium j, k, q Part of secondary synnema l Phialides m Conidia bound by deliquescing mucilaginous material n–p Conidia. Scale bars: 1 cm (c, d), 1000 µm (e), 200 µm (f, h, q), 100 µm (g, i), 50 µm (j), 20 µm (k, l), 10 µm (m–p).
growing on PDA, reaching 2 cm diam., after 4 weeks at 25 °C,with circular, dense mycelium on the surface. After 6 weeks, the colour of the colony gradually deepened from white to dark brown from the periphery to the centre, with complex fold as 4 circle rings, reverse white to yellow in colour, with ring. Synnemata was produced after 8 weeks. Most of the characters are the same as the fresh collection except phialides and mucilaginous spheres. Phialides 56–86 µm long (x̄ = 71 µm, n = 60), 3–5 µm wide at base (x̄ = 4 µm, n = 60), 1.4–2.2 µm at top (x̄ = 1.8 µm, n = 60), hirsutella-like, hyaline, solitary, unbranched, narrow slender, smooth, 1–4 septa, not observed on host. Mucilaginous spheres 10.5–15.9 × 8.2–14.7 µm (x̄ = 12.7 × 11.5 µm, n = 60), 1–4 conidia, hyaline to brown. Observation stopped after 10 weeks.
THAILAND, Chiang Mai, The Mushroom Research Centre, on dead larva of Elateridae, Coleoptera, 6.5 cm long 0.38 cm diam., brown to dark brown, with thallus inside (larva), 18 July 2015, YuanPin Xiao, (MFLU 18–0658, holotype); THAILAND, Chiang Mai, The Mushroom Research Centre, on dead larva of Elateridae, Coleoptera, 5.8 cm long 0.4 cm diam., brown to dark brown, with thallus inside (larva), 22 August 2015, YuanPin Xiao, (MFLU 18–0659, paratypes, ex-type living culture, MFLUCC 18–0492); THAILAND, Chiang Mai, Samoeng on larva insect of Elateridae, Coleoptera, 5.5 cm long 0.32 cm diam., brown to dark brown, with thallus inside (larva), 18 June 2017, YuanPin Xiao, (MFLU 18–0660, paratypes, living culture, MFLUCC 18–0493, MFLUCC 18–0494).
Ophiocordyceps sporangifera is closely related to O. myrmicarum D.R. Simmons & Groden in our phylogenetic tree (Fig.
Ophiocordyceps sporangifera (culture) MFLUCC 18–0492. a Upper side of the culture b Reverse side of the culture c, d Synnemata growing on PDA medium e, g Synnemata f Mycelium h–j Phialides k Conidia l–n Conidia form mucilaginous spheres. Scale bars: 1 cm (a, b), 5000 µm (c), 1000 µm (d), 500 µm (e), 100 µm (f, g), 50 µm (h–j), 10 µm (k–n).
Species | Ophiocordyceps myrmicarum | Ophiocordyceps sporangifera |
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Host | Myrmica rubra (Hymenoptera) | Elateridae, Coleoptera |
Primary synnemata | Whitish-yellow aging to rufous brown | 9–18 cm high 1–2 mm diam., brown to deep brown |
Secondary synnemata (µm) | Hyaline aging to rufous brown, up to 350 long, narrow (25) at base, common on agar but not observed on host | Brown to white, not smooth 1092–1937 × 21–34, arising from the all parts of the primary synnemata, observed on both of the host and agar |
Primary phialides (µm) | Subulate, hyaline or pigmented at base, 39.9–86.2 long, 3.6–5.4 wide at base | Slender, solitary, hyaline, unbranched, narrow, smooth, 25–40 × 1.3–2.5 |
Secondary phialides (µm) | Subulate, 27.2–47.0 long, 2.4–3.3 wide at base | Narrow slender, 56–86 long, 3–5 wide at base, 1.4–2.2 at top, 1–4 septa, common on culture but not observed on host |
Sporangium (µm) | No observed | 78–121 diam., spherical, white immature, brown after mature |
Conidia (µm) | 7.3–9.6 × 3.2–5.1 reniform to ovoid, bi-guttulate, aseptate | 6.7–9.8 × 2.5–3.8, subglobose to reniform |
Mucilaginous spheres (µm) | Composed of 1–4 conidia, hyaline to brown, at phialide apex | 10.5–12.9 × 6.4–8.7, composed of 1–12 conidia, hyaline on host, 1–4 conidia on culture, hyaline to brown on culture |
Reference |
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This study |
We introduce two new entomopathogenic species of Ophiocordyceps, one from Coleoptera (Elateridae) and the other from flies (Diptera). Morphological and phylogenetic analyses have provided insights to resolve generic delimitation (
Ophiocordyceps globiceps groups with H. dipterigena, O. dipterigena, Ophiocordyceps sp. and O. hemisphaerica in the phylogenetic tree with high bootstrap support, while four of these species are reported as fly (Diptera) parasitic fungi (
Ophiocordyceps sporangifera is an asexual morph species and groups with O. myrmicarum in the phylogenetic tree (Fig.
This work was jointly supported by the National Natural Science Foundation of China (No. 31460012 & 31760014) and the Science and Technology Foundation of Guizhou Province (No. [2016]2863 & [2017]5788). Yuanpin Xiao also thanks the future of specialist fungi in a changing climate: baseline data for generalist and specialist fungi associated with ants, Rhododendron species and Dracaena species (grant no: DBG6080013), Impact of climate change on fungal diversity and biogeography in the Greater Mekong Subregion (grant no: RDG6130001) and the Mushroom Research Foundation, Chiang Rai, Thailand for supporting this research.