Research Article |
Corresponding author: Sheng-Hua Wu ( shwu@mail.nmns.edu.tw ) Academic editor: Bao-Kai Cui
© 2018 Sheng-Hua Wu, Che-Chih Chen, Chia-Ling Wei.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Wu S-H, Chen C-C, Wei C-L (2018) Three new species of Phanerochaete (Polyporales, Basidiomycota). MycoKeys 41: 91-106. https://doi.org/10.3897/mycokeys.41.29070
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Phanerochaete canobrunnea, P. cystidiata and P. fusca are presented as new species, supported by morphological studies and two sets of phylogenetic analyses. The 5.8S+nuc 28S+rpb1 dataset shows the generic placement of the three species within the phlebioid clade of Polyporales. The ITS+nuc 28S dataset displays relationships for the new taxa within Phanerochaete s.s. Phanerochaete canobrunnea grew on angiosperm branches in subtropical Taiwan and is characterised by greyish brown hymenial surface, brown generative hyphae and skeletal hyphae and absence of cystidia. Phanerochaete cystidiata grew on angiosperm branches above 1000 m in montane Taiwan and SW Yunnan Province of China and is characterised by cream to yellowish hymenial surface and more or less encrusted leptocystidia. Phanerochaete fusca grew on angiosperm branches at 1700 m in Hubei Province of China and is characterised by dark brown hymenial surface, leptocystidia, brown subicular hyphae and colourless to brownish basidiospores.
China, corticioid fungi, multi-marker phylogeny, Phanerochaetaceae , Taiwan
The genus Phanerochaete P. Karst., typified by P. alnea (Fr.) P. Karst., belongs to the Polyporales of the Basidiomycota and encompasses, when taken in a broad sense (
Phanerochaete recently has been shown to be a polyphyletic group, containing members placed throughout the phlebioid clade of Polyporales (
The field survey of the corticioid fungi from Taiwan and mainland China conducted in 2014, 2015 and 2017, have revealed three new species of Phanerochaete s.s. presented herein, based on morphological and phylogenetic evidence.
Voucher specimens are deposited at the herbarium of National Museum of Natural Science of ROC (TNM). We used three mounting media for microscopic studies: 5% potassium hydroxide (KOH) with 1% phloxine was used for observation and measurements; Melzer’s reagent (IKI) was utilised to determine amyloidity and dextrinoidity and Cotton blue (CB) was utilised to check cyanophily. A standard method of measurement for microscopic characters follows
Dried specimens or mycelia were first ground into a fine powder using liquid nitrogen and a TissueLyser II (Qiagen, Hilden, Germany). DNA was then extracted using the Plant Genomic DNA Extraction Miniprep System (Viogene-Biotek Corp., New Taipei, Taiwan) according to the manufacturer’s instructions. The rDNA ITS1-5.8S-ITS2 (ITS) was amplified using primer pairs ITS1/ITS4 (
Species and sequences used in the phylogenetic analyses. Newly generated sequences are shown in bold.
Taxon | Strain/Specimen | ITS (contains 5.8S) | nuc 28S | rpb1 |
---|---|---|---|---|
Bjerkandera adusta | HHB-12826-Sp | KP134983 | KP135198 | KP134784 |
Byssomerulius corium | FP-102382 | KP135007 | KP135230 | KP134802 |
Candelabrochaete africana | FP-102987-Sp | KP135294 | KP135199 | KP134872 |
Ceraceomyces serpens | HHB-15692-Sp | KP135031 | KP135200 | KP134785 |
Ceriporia alachuana | FP-103881-Sp | KP135341 | KP135201 | KP134845 |
Ceriporia purpurea | KKN-223-Sp | KP135044 | KP135203 | KP134788 |
Efibula americana | FP-102165 | KP135016 | AY684165 | AY864873 |
Emmia lacerata | FP-55521-T | KP135024 | KP135202 | KP134805 |
Gloeoporus pannocinctus | L-15726-Sp | KP135060 | KP135214 | KP134867 |
Hydnophlebia chrysorhiza | FD-282 | KP135338 | KP135217 | KP134848 |
Hyphoderma litschaueri | FP-101740-Sp | KP135295 | KP135219 | KP134868 |
Hyphoderma mutatum | HHB-15479-Sp | KP135296 | KP135221 | KP134870 |
Hyphodermella rosae | FP-150552 | KP134978 | KP135223 | KP134823 |
Meruliopsis alnbostramineus | HHB-10729 | KP135051 | KP135229 | KP134787 |
Phaeophlebiopsis peniophoroides | FP-150577 | KP135417 | KP135273 | KP134813 |
Phanerochaete aculeata | Wu 880701-2 | – | GQ470636 | – |
Phanerochaete affinis | KHL11839 | EU118652 | EU118652 | – |
Phanerochaete alnea | OM8110 | KP135171 | – | – |
Phanerochaete arizonica | RLG-10248-Sp | KP135170 | KP135239 | KP134830 |
Phanerochaete australis | HHB-7105-Sp | KP135081 | KP135240 | KP134840 |
Phanerochaete bambusicola | Wu 0707-2 | MF399404 | MF399395 | LC314324 |
Phanerochaete brunnea | He1873 | KX212220 | KX212224 | – |
Phanerochaete burtii | HHB-4618 | KP135117 | KP135241 | KP134829 |
Phanerochaete calotricha | Vanhanen-382 | KP135107 | – | KP134826 |
Phanerochaete canobrunnea | CHWC 1506-17 | LC412093 | LC412102 | – |
CHWC 1506-39 | LC412094 | LC412103 | – | |
CHWC 1506-66 | LC412095 | LC412104 | – | |
Phanerochaete carnosa | HHB-9195-Sp | KP135129 | KP135242 | KP134831 |
Phanerochaete chrysosporium | HHB-6251-Sp | KP135094 | KP135246 | KP134842 |
Phanerochaete citrinosanguinea | FP-105385 | KP135100 | KP135234 | KP134824 |
Phanerochaete concrescens | LE < RUS>:287,008 | KP994375 | – | – |
Phanerochaete cumulodentata | H:6,033,465 | LN833868 | – | – |
VL212 | JF440574 | – | – | |
Phanerochaete cystidiata | GC 1708-358 | LC412096 | LC412101 | LC412107 |
Wu 1708-326 | LC412097 | LC412100 | LC412108 | |
Phanerochaete ericina | HHB-2288 | KP135167 | KP135247 | KP134834 |
Phanerochaete exilis | HHB-6988 | KP135001 | KP135236 | KP134799 |
Phanerochaete fusca | Wu 1409-161 | LC412098 | LC412105 | LC412109 |
Wu 1409-163 | LC412099 | LC412106 | LC412110 | |
Phanerochaete incarnata | WEI 16-078 | MF399407 | MF399398 | LC314327 |
Phanerochaete krikophora | HHB-5796-Sp | KP135164 | KP135268 | KP134837 |
Phanerochaete laevis | HHB-15519-Sp | KP135149 | KP135249 | KP134836 |
Phanerochaete livescens | FD-106 | KP135070 | KP135253 | KP134841 |
Phanerochaete magnoliae | HHB-9829-Sp | KP135089 | KP135237 | KP134838 |
Phanerochaete odontoidea | Wu 9310-8 | MF399408 | MF399399 | LC314328 |
Phanerochaete porostereoides | He1902 | KX212217 | KX212221 | – |
He1908 | KX212218 | KX212222 | – | |
Phanerochaete pseudomagnoliae | PP-25 | KP135091 | KP135250 | KP134839 |
Phanerochaete pseudosanguinea | FD-244 | KP135098 | KP135251 | KP134827 |
Phanerochaete rhodella | FD-18 | KP135187 | KP135258 | KP134832 |
Phanerochaete robusta | Wu 1109-69 | MF399409 | MF399400 | LC314329 |
Phanerochaete sacchari | Wu 880313-6 | – | GQ470654 | – |
Phanerochaete sanguinea | HHB-7524 | KP135101 | KP135244 | KP134825 |
Phanerochaete sanguineocarnosa | FD-359 | KP135122 | KP135245 | KP134828 |
Phanerochaete sordida | FD-241 | KP135136 | KP135252 | KP134833 |
Phanerochaete stereoides | VPCI207312 | KF291012 | – | – |
Wu 9708-118 | – | GQ470661 | – | |
Phanerochaete subceracea | FP-105974-R | KP135162 | KP135255 | KP134835 |
Phanerochaete subodontoidea | Wu 0106-35 | MF399411 | MF399402 | LC314331 |
Phanerochaete taiwaniana | Wu 0112-13 | MF399412 | MF399403 | LC314332 |
Phanerochaete thailandica | 2015_07 | MF467737 | – | – |
Phanerochaete velutina | Kotiranta21402 | KP135179 | – | – |
Phlebia centrifuga | HHB-9239-Sp | KP135380 | KP135262 | KP134844 |
Phlebia chrysocreas | HHB-6333-Sp | KP135358 | KP135263 | KP134861 |
Phlebia fuscoatra | HHB-10782-Sp | KP135365 | KP135265 | KP134857 |
Phlebia radiata | AFTOL-484 | AY854087 | AF287885 | AY864881 |
Phlebia uda | FP-101544-Sp | KP135361 | KP135232 | KP134859 |
Phlebiopsis gigantea | FP-70857-Sp | KP135390 | KP135272 | KP134821 |
Pirex concentricus | OSC-41587 | KP134984 | KP135275 | KP134843 |
Rhizochaete radicata | FD-123 | KP135407 | KP135279 | KP134816 |
Scopuloides rimosa | HHB-7042 | KP135350 | KP135282 | KP134853 |
Terana caerulea | FP-104073 | KP134980 | KP135276 | KP134865 |
We included two datasets for phylogenetic analyses. The 5.8S+nuc 28S+rpb1 was compiled for inferring generic classification of target species within the phlebioid clade of Polyporales. The ITS+nuc 28S was compiled for getting better resolutions on species level within Phanerochaete s.s. The selection of strains and species consulted
For both datasets, Maximum Likelihood (ML) and Bayesian Inference (BI) analyses were performed, respectively, using RAxML BlackBox (Stamatakis et al. 2014) and MrBayes v. 3.2.6 (
The 5.8S+nuc 28S+rpb1 dataset consisted of 58 sequences of 2481 characters including gaps, of which 931 sites were parsimony informative. The ITS+nuc 28S dataset consisted of 45 sequences of 2199 characters including gaps, of which 220 sites were parsimony informative. Topologies of phylogenetic trees of each dataset inferred from BI and ML methods were similar and, thus, only ML trees were shown (Figs
Phylogram inferred from Maximum likelihood analysis of the concatenated 5.8S+nuc 28S+rpb1 dataset of representative taxa in the phlebioid clade of Polyporales. Branches are labelled with Maximum likelihood bootstrap values ≥70% and Bayesian posterior probabilities ≥0.9. Studied taxa are shaded with greyish boxes. Scale bar = substitutions per site.
Phylogram inferred from Maximum likelihood analysis of the concatenated ITS+nuc 28S dataset of taxa in Phanerochaete s.s. Nodes are labelled with Maximum likelihood bootstrap values ≥70% and Bayesian Posterior probabilities ≥0.9. Studied taxa studied are shaded with greyish boxes. Scale bar = substitutions per site.
Phanerochaete canobrunnea is recognised by brown generative hyphae and brown skeletal hyphae, in combination with absence of cystidia.
TAIWAN. Nantou County: Yuchih Township, Lienhuachih, 23°55'N, 120°53'E, 715 m alt., on angiosperm branch, coll. W.C. Chen, C.C. Chen & C.L. Wei, 23 Jun 2015, CHWC 1506-17 (TNM F0029207).
From canus+brunneus (= greyish-brown), referring to the colour of the hymenial surface.
Basidiome resupinate, effuse, loosely adnate, membranaceous, 250–500 μm thick in section. Hymenial surface pale greyish-brown, slightly darkening in KOH, smooth, sometimes cracked; margin concolorous or brownish, slightly fibrillose or determinate.
Hyphal system dimitic; generative hyphae mostly simple-septate, single or double clamp connections occasionally present in subiculum. Subiculum fairly uniform, with fairly loose texture, 200–400 μm thick; generative hyphae interwoven, brown, more or less straight, moderately ramified, rarely encrusted, 4–9 (–11) μm diam., thin- to thick-walled, walls up to 1.5 μm thick, anastomoses occasional; skeletal hyphae interwoven, brown, more or less straight, un-ramified or ramified, 2–5 μm diam., usually subsolid or thick-walled, walls up to 1.5 μm, adventitious septa occasionally present. Hymenial layer thickening, with dense texture, 50–100 μm thick; hyphae more or less vertical, brownish to subcolourless, 3–6 μm diam., thin-walled. Cystidia lacking. Basidia subclavate to clavate, 15–25 × 5–6 μm, 4-sterigmate. Basidiospores ellipsoid to narrowly ellipsoid, adaxially flattened, smooth, thin-walled, IKI –, CB –, mostly 4.2–5.8 × 2.5–3 μm. [(4–) 4.5–5.8 (–6) × (2.5–) 2.7–3 (–3.2) μm, L = 5.10±0.54 μm, W = 2.86±0.18 μm, Q = 1.78 (n = 30) (CHWC 1506-17); (4–) 4.2–5 (–5.8) × (2.3–) 2.5–2.8 (–3) μm, L = 4.63±0.42 μm, W = 2.66±0.17 μm, Q = 1.75 (n = 30) (CHWC 1506-39)].
TAIWAN. Nantou County: Yuchih Township, Lienhuachih, 23°55'N, 120°53'E, 715 m alt., on angiosperm branch, coll. W.C. Chen, C.C. Chen & C.L. Wei, 23 Jun 2015, CHWC 1506-39 (TNM F0029217); CHWC 1506-66 (TNM F0029236).
Known from subtropical Taiwan.
Remarks. Amongst the few species in Phanerochaete having brown subicular hyphae, only P. canobrunnea and P. thailandica possess skeletal hyphae [described as “quasi-binding hyphae” in the protologue of P. thailandica,
Phanerochaete cystidiata is characterised by having a fibrillose margin of the basidiome and apically narrow or tapering leptocystidia that are more or less encrusted. Additionally, crystal masses are present in the hymenial layer.
TAIWAN. Nantou County: Aowanta, 23°57'N, 121°10'E, 1200 m alt., on angiosperm branch, coll. C.C. Chen, 28 Aug 2017, GC 1708-358 (TNM F0031801).
From cystidiatus, referring to the presence of cystidia of this species.
Basidiome resupinate, effuse, adnate, membranaceous, 120–250 (–330) μm thick in section. Hymenial surface creamish-yellow, brownish in KOH, smooth to occasionally slightly tuberculate (due to crystal masses in hymenial layer), sometimes cracked; margin whitish or concolorous, fibrillous to fimbriate, occasionally determinate.
Hyphal system monomitic; hyphae simple-septate, clamp connections rarely present in subiculum. Subiculum fairly uniform, with somewhat loose to fairly dense texture, usually very dense near the substrate, 70–150 μm thick; hyphae more or less horizontal, colourless, fairly straight, moderately ramified, occasionally strongly encrusted with crystals, 3–6 (–7) μm diam., with 0.8–1.5 μm thick walls, anastomoses occasional. Hymenial layer thickening, with fairly dense texture, 50–100 (–180) μm thick, occasionally stratified; hyphae more or less vertical, colourless, 2.5–5 μm diam., thin-walled. Crystal masses occasionally abundant in hymenial layer. Leptocystidia numerous, immersed or emergent, cylindrical, median part usually slightly swollen and slightly thick-walled, with narrow or tapering apices, sparsely to heavily encrusted, (35–) 40–60 × 4–5.5 μm. Basidia subclavate to narrowly clavate, usually guttulate when mature, 20–30 × 4.5–5.5 μm, 4-sterigmate. Basidiospores ellipsoid to narrowly ellipsoid, adaxially flattened, smooth, thin-walled, guttulate, IKI–, CB–, mostly 4–5.3 × 2.5–3 μm. [4–5 (–5.5) × (2.5–) 2.7–3 (–3.3) μm, L = 4.59±0.43 μm, W = 2.86±0.18 μm, Q = 1.61 (n = 30) (GC 1708-358); (4–) 4.2–5 (–5.5) × 2.5–3 (–3.2) μm, L = 4.72±0.40 μm, W = 2.79±0.20 μm, Q = 1.70 (n = 30) (Wu 1708-326)].
CHINA. Yunnan Province: Wenshan Zhuang and Miao Autonomous Prefecture, Maguan County, Dalishu Township, Lake, 23°07'04"N, 104°08'17"E, 1800 m alt., on angiosperm branch, coll. C.C. Chen, 7 Aug 2017, GC 1708-76 (TNM F0031803). TAIWAN. Nantou County: Aowanta, 23°57'N, 121°10'E, 1200 m alt., on angiosperm branch, coll. S.H. Wu, 28 Aug 2017, Wu 1708-326 (TNM F0031802).
Known from China (Yunnan Province) and Taiwan (type locality).
Phanerochaete ericina is the most closely related species (Figs
Phanerochaete fusca is characterised by smooth to tuberculate dark brown hymenial surface, monomitic hyphal system with brown subicular hyphae and leptocystidia with narrow or tapering apices. Additional diagnostic features: hyphae and cystidia usually with adventitious septa, subicular hyphae sometimes swollen at hyphal ends and basidia becoming thick-walled and brownish when old.
CHINA, Hubei Province: Shennongjia Forest Area, Wenshui Forest Farm, 31°44'N, 110°20'E, 1700 m alt., on angiosperm branch, coll. S.H. Wu, 19 Sep 2014, Wu 1409-161 (TNM F0029722).
From fuscus (= dark brown), referring to the colour of the hymenial surface.
Basidiome resupinate, effuse, adnate, membranaceous, 250–580 μm thick in section. Hymenial surface dark brown, slightly darkening in KOH, smooth to tuberculate, not cracked; margin concolorous, more or less separable, determinate.
Hyphal system monomitic; hyphae simple-septate, clamp connections rarely present in subiculum. Subiculum fairly uniform, with dense texture, 200–480 μm thick; hyphae more or less horizontal, brown, fairly straight, moderately ramified, usually swollen at hyphal ends, usually encrusted near subhymenium, (2.5–) 3–7 (–7.5) μm diam., with slightly thick to up to 2 μm thick walls, with small oily drops, usually with adventitious septa. Hymenial layer thickening, with dense texture, 50–100 μm thick; hyphae more or less vertical, brownish to subcolourless, 2.5–4 μm diam., slightly thick-walled. Leptocystidia numerous, originating from hymenial layer, projecting, cylindrical with narrow or tapering apices, sometimes encrusted, subcolourless to brownish, usually with 1 or 2 adventitious septa, 50–70 × 3.5–5.5 (–6) μm, with thin to up to 1 μm thick walls. Basidia clavate or occasionally narrowly clavate, subcolourless to brownish, sometimes with an adventitious septum, 22–50 × 5–6 μm, with thin to up to 1 μm thick walls, 4-sterigmate. Basidiospores narrowly ellipsoid to subcylindrical, adaxially slightly concave, smooth, thin- to slightly thick-walled, colourless to sometimes brownish, IKI –, CB –, mostly 5.7–7.3 × 3–3.5 μm. [(5.3–) 5.7–7.3 (–7.8) × (2.8–) 3–3.5 (–3.7) μm, L = 6.63±0.64 μm, W = 3.24±0.28 μm, Q = 2.05 (n = 30) (Wu 1409-161)].
CHINA. Hubei Province: Shennongjia Forest Area, Wenshui Forest Farm, 31°44'N, 110°20'E, 1700 m alt., on angiosperm branch, coll. S.H. Wu, 19 Sep 2014, Wu 1409-163 (TNM F0029723).
Known from China (Hubei Province).
Phanerochaete stereoides Sheng H. Wu resembles P. fusca in having brown subicular hyphae and leptocystidia. However, hymenial surface of the former is pale greyish-brown, while the latter is dark brown. Moreover, cystidia of P. stereoides are uniformly thin-walled and colourless, not with 1 or 2 adventitious septa. These two species are not closely related according to the phylogenetic analyses (Fig.
This study was financed by Ministry of Science and Technology of Taiwan (ROC) (Grant no 107-2621-B-178-002-MY3). The authors thank Dr. Xiang-Hua Wang (Kunming Institute of Botany, China) for providing help in the field trips in SW Yunnan Province, China. We are also grateful to Ms. Siou-Zhen Chen (TNM) for managing studied specimens and to Shin-Yi Ke (TNM) for the help in DNA sequencing work.