Research Article |
Corresponding author: Fang Wu ( fangwubjfu2014@yeah.net ) Academic editor: Alfredo Vizzini
© 2018 Tie-Zhi Liu, Qian Chen, Mei-Ling Han, Fang Wu.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Liu T-Z, Chen Q, Han M-L, Wu F (2018) Fomitiporia rhamnoides sp. nov. (Hymenochaetales, Basidiomycota), a new polypore growing on Hippophae from China. MycoKeys 36: 35-43. https://doi.org/10.3897/mycokeys.36.25986
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Based on morphology and phylogenetic analysis, Fomitiporia rhamnoides sp. nov. is described. It is characterised by perennial, pileate basidiomata, distinctly shining poroid surface, a zonate context, 11–13 pores per mm, parallel tramal hyphae and regularly arranged contextual hyphae, the presence of cystidioles, globose, hyaline, thick-walled, smooth, dextrinoid, strongly cyanophilous basidiospores measuring 5.8–7 × 5.4–6.5 µm and growing on Hippophae rhamnoides in northern China. Fomitiporia rhamnoides differs from other Fomitiporia species growing on Hippophae by its smaller pores (11–13 per mm vs. <10 per mm).
Hymenochaetaceae , taxonomy, wood-inhabiting fungi
Fomitiporia Murrill (Murrill 1907), typified by F. langloisii Murrill, is an important genus in Hymenochaetaceae because some species are pathogens of trees (
During investigations on wood-inhabiting fungi in northern China, in Hebei and Shanxi provinces, some specimens of a Fomitiporia species were collected on living Hippophae rhamnoides. They are characterised by distinctly small pores which make them different from other Fomitiporia species growing on Hippophae.
To understand their taxonomic placement, phylogenetic analysis was carried out based on the nuc rDNA regions of the 5.8S rDNA (ITS) and nuc 28S rDNA D1-D2 domains. Molecular analyses showed that the sampled specimens are clustered into a lineage representing an unknown species of Fomitiporia.
The studied specimens are deposited at the herbarium of the Institute of Microbiology, Beijing Forestry University (BJFC). The microscopic procedure follows
CTAB rapid plant genome extraction kit-DN14 (Aidlab Biotechnologies Co. Ltd, Beijing) was used to obtain PCR products from dried specimens according to the manufacturer’s instructions with some modifications. Two DNA gene fragments, ITS and 28S were amplified using respectively the primer pairs ITS5/ITS4 (
Sequences generated for this study and additional sequences downloaded from GenBank were aligned using BioEdit (
In the study, nuclear ribosomal RNA genes were used to determine the phylogenetic position of the new species. Phellinus uncisetus Robledo, Urcelay & Rajchenb. was designated as an outgroup following
Maximum parsimony analysis was applied to the combined dataset of ITS+28S sequences using PAUP* version 4.0b10 (
MrModeltest 2.3 (
The combined ITS+28S dataset includes 78 specimens and resulted in an alignment of 1737 characters, of which 1124 characters are constant, 98 are variable and parsimony-uninformative and 515 are parsimony-informative. Maximum parsimony analysis yielded 28 equally parsimonious trees (TL = 1515, CI = 0.549, HI = 0.451, RI = 0.813, RC = 0.446). The best model for the combined dataset, estimated and applied in the Bayesian analysis, is GTR+I+G, lset nst = 6, rates = invgamma; prset statefreqpr = dirichlet (1,1,1,1). Bayesian analysis and ML analysis resulted in a similar topology to MP analysis, with an average standard deviation of split frequencies = 0.007191 (BI). Therefore, only the MP tree was presented and BP, BS and BPP values simultaneously above 50%, 50% and 0.95, respectively, were indicated at the nodes (Fig.
Phylogenetic tree inferred from maximum parsimony (MP) analysis based on the combined dataset of ITS and 28S. Only maximum parsimony (BP), maximum likelihood (BS) and Bayesian posterior probabilities (BPP) greater than or equal to 50% (BP), 50% (BS) and 0.95 (BPP) are reported on the branches.
CHINA. Hebei Province, Zuolu County, Xiaowutai Nature Reserve, Shanjiankou, on living tree of Hippophae rhamnoides, 10.IX.2017, Dai 18091 (BJFC025621).
Rhamnoides (Lat.) refers to the species growing on Hippophae rhamnoides.
Basidiomata perennial, pileate, solitary or a few imbricated, hard corky and without odour or taste when fresh, woody hard and medium in weight when dry; pilei dimidiate to ungulate, triquetrous in section, projecting up to 5 cm, 7 cm wide and 2.5 cm thick at base; pileal surface yellowish-brown, greyish-brown to dark brown, concentrically sulcate, at first velutinate, becoming glabrous and slightly cracked with age; margin obtuse. Poroid surface clay-buff to yellowish-brown when fresh, becoming orange brown to snuff brown when dry, shining; sterile margin yellowish-brown, up to 3 mm wide; pores circular, 11–13 per mm, dissepiments entire. Context yellowish-brown, zonate, woody hard, up to 1.5 cm thick; tubes greyish-brown, paler than context, hard corky to brittle, up to 1 cm long, annual layers indistinct.
Hyphal system dimitic; generative hyphae simple septate; tissue darkening but otherwise unchanged in KOH.
Generative hyphae hyaline to pale yellow, thin- to slightly thick-walled, occasionally branched, frequently septate, 3–4 µm in diam., skeletal hyphae yellowish-brown, thick-walled with a wide lumen, unbranched, occasionally septate, straight, regularly arranged, 4.5–6 µm in diam.
Generative hyphae hyaline to pale yellowish, thin-walled, occasionally branched, frequently septate, 2–3 µm in diam., skeletal hyphae dominant, yellowish-brown, thick-walled with a wide lumen, unbranched, occasionally septate, straight, parallel along the tubes, 2.5–4 µm in diam. Setae absent; cystidioles present, more or less ventricose, hyaline, thin-walled, 12–20 × 3–6 μm; basidia subglobose to barrel-shaped, with four sterigmata and a simple septum at the base, 8–16 × 6–10 µm; basidioles dominant in hymenium, in shape similar to basidia, but slightly smaller; big rhomboid crystals present amongst hymenium.
Basidiospores globose, hyaline, thick-walled, smooth, dextrinoid in Melzer’s reagent, strongly CB+, (5.2–)5.8–7(–7.3) × (5–)5.5–6.5(–6.8) µm, L = 6.47 µm, W = 6.06 µm, Q = 1.06–1.08 (n=60/2).
CHINA. Hebei Province, Zuolu County, Xiaowutai Nature Reserve, Shanjiankou, on living tree of Hippophae rhamnoides, 10.IX.2017, Dai 18087 (BJFC025617), Dai 18088 (BJFC025618), Dai 18090 (BJFC025620), Dai 18100 (BJFC025630), Dai 18101 (BJFC25631). Shanxi Province, Zuoyun County, Santun, on living tree of Hippophae rhamnoides, 19.V.2017, Dai 17368 (BJFC024903), Dai 17369 (BJFC024904), Dai 17370 (BJFC024905).
Fomitiporia rhamnoides is characterised by its very small pores (11–13 per mm) and growing on Hippophae rhamnoides. It has the same sequences of Fomitiporia guoshangensis, an illegitimate name (art. 7, 8, 32A, code of nomenclature) also described based in Chinese collections (
Three species of Fomitiporia, F. hippophaëicola (H. Jahn) Fiasson & Niemelä, F. norbulingka B.K. Cui & Hong Chen, F. subhippophaëicola B.K. Cui & H. Chen, have been reported on Hippophae (
Fomitiporia rhamnoides resembles F. hippophaëicola, F. norbulingka and F. subhippophaëicola by sharing similar basidiomata and basidiospores, but it can be distinguished from these three species by smaller pores (11–13 per mm, vs. 5–7 per mm in F. hippophaëicola, 6–9 per mm in F. norbulingka, 8–10 per mm in F. subhippophaëicola). Phylogenetically, F. rhamnoides forms a single lineage and is closely related to F. norbulingka.
Fomes yasudae Lloyd was originally described from Japan on an angiosperm trunk (
We would like to express our deep thanks to Prof. Yu-Cheng Dai (Beijing Forestry University) who allowed us to study his specimens. This study was supported by the National Natural Science Foundation of China (Nos. 31700024 and 31760004).