Research Article |
Corresponding author: Alfredo Vizzini ( alfredo.vizzini@unito.it ) Academic editor: María P. Martín
© 2018 Alfredo Vizzini, Claudio Angelini, Cristiano Losi, Enrico Ercole.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Vizzini A, Angelini C, Losi C, Ercole E (2018) Diversity of polypores in the Dominican Republic: Pseudowrightoporia dominicana sp. nov. (Hericiaceae, Russulales). MycoKeys 34: 35-45. https://doi.org/10.3897/mycokeys.34.25371
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The new species Pseudowrightoporia dominicana is described from the Dominican Republic based on morphological and molecular data (nrITS and nrLSU sequence analyses). It is mainly characterised by pileate basidiomata with a bright pinkish context and a di-trimitic hyphal system. Phylogenetically, it is sister to the African species P. gillesii and to the Asiatic P. japonica.
Basidiomycota , Agaricomycetes , Caribbean Islands, Polypores, Phylogeny, Taxonomy
The genus Wrightoporia Pouzar, typified with W. lenta (Overh. & J. Lowe) Pouzar (
During the species diversity study of wood-inhabiting macromycetes in the Dominican Republic, a pileate Pseudowrightoporia was discovered. The aim of this investigation was to identify and to analyse the Pseudowrightoporia specimens using both morphological and molecular techniques.
Photographs of fresh basidiomata were taken in situ by a Nikon Coolpix 8400 digital camera and then dried, while the photos of the microscopical structures were obtained through a Olympus BH-2 light microscope and a Nikon D7100 digital camera. For microscopical analysis, tiny fragments from dried material were mounted in Melzer’s anionic reagent for testing amyloid and dextrinoid reactions of spores and other microscopical elements. All microscopic measurements were carried out with a ×1000 oil immersion objective. Basidiospores were measured from hymenophores of mature basidiomes, dimensions are given as: (minimum–) average minus standard deviation – average – average plus standard deviation (–maximum) of length × (minimum–) average minus standard deviation – average – average plus standard deviation (–maximum) of width; Q = (minimum–) average minus standard deviation – average – average plus standard deviation (–maximum) of the length/width ratio. Spore statistics were produced using R version 3.4.4 (
Genomic DNA was isolated from 10 mg of a dried voucher specimen (JBSD 127410), using the DNeasy Plant Mini Kit (Qiagen, Milan) according to the manufacturer’s instructions. Primers LR0R/LR6 (
Bayesian phylogram obtained from the combined nrITS-nrLSU sequence alignment of Russulales taxa selected according to
Sequences were checked and assembled with Geneious 5.3 (
Two phylogenetic analyses were performed: the first, based on a combined nrITS and nrLSU sequences dataset, to focus on the phylogenetic position of the new species in the Russulales (Russuloid clade); the second, based only on a nrITS dataset was restricted to the taxa closely related to P. dominicana according with the previous combined data analysis. Alignments were generated for each nrITS and nrLSU dataset using MAFFT (
Bayesian phylogram obtained from the nrITS sequence alignment of Pseudowrightoporia and Wrightoporiopsis species. Dentipellis coniferarum, D. fragilis and Hericium alpestre were used as outgroup taxa. Values for clades that are supported in either the Bayesian (posterior probabilities, BPP) and maximum likelihood (ML bootstrap percentage, MLB) analyses are indicated. BPP values (in bold) above 0.70 and MLB values above 50% are given above/below branches. The newly sequenced collection is in bold.
The combined nrITS and nrLSU data matrix comprised 118 sequences (including 117 from GenBank) and includes 2132 positions. The nrITS data matrix comprises a total of 25 sequences (including 24 from GenBank) and includes 687 positions. As both Bayesian and Maximum likelihood analyses produced comparable topologies, only the Bayesian trees with both BPP and MLB values are shown (Figs
Dominican Republic. La Vega (Province), Jarabacoa (Municipality), Montaña (Locality), 19°06'39"N, 70°37'57"W, on an unidentified live trunk of a deciduous tree, in a mixed mountain forest with several broadleaved species and pines (Pinus occidentalis), 17 December 2016, Claudio Angelini, (JBSD 127410, isotype ANGE 789).
The epithet refers to the country, The Dominican Republic, where this species was found.
Basidiomata annual, pileate, sessile, single or in small clusters, fibrous-tough (Fig.
Pileate, gregarious on a live trunk of deciduous tree, so far known only from the type locality.
All the phylogenetic analyses show P. dominicana to be a distinct lineage in the genus Pseudowrightoporia (Figs
Pseudowrightoporia gillesii and P. japonica are the species phylogenetically most closely related to P. dominicana (Figs
Amongst the morphologically most similar species to P. dominicana, Wrightoporia dimidiata A. David & Rajchenb. from Asia (Singapore) is distinguished by a hymenophore with 3–4 pores per mm, dimitic hyphal system, spores measuring 3.5–4 × 3 µm and presence of cystidiola, gloeocystidia and gloeopleurous hyphae (
We are grateful to Leif Ryvarden (University of Oslo) for his valuable comments and suggestions on the new species and to D. Jean Lodge (USDA Forest Service, Luquillo, Puerto Rico) for improving the English text. We also wish to thank Ricardo G. García, Francisco Jiménez, Brígido Peguero, Yuley E. Piñeyro and Alberto Veloz (Jardín Botánico Nacional Dr. Rafael M. Moscoso, Santo Domingo, Dominican Republic) for their interest and encouragement to study the fungi of the Dominican Republic and for their active cooperation in putting at our disposal herbarium material of this institution.