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An updated checklist of the lichens of St. Eustatius, Netherlands Antilles
expand article infoAndre Aptroot, Michael Stech§
‡ ABL Herbarium, Baarn, Netherlands
§ Naturalis Biodiversity Center, Leiden, Netherlands
Open Access

Abstract

In the course of a multi-taxon biodiversity inventory for the island of St. Eustatius, lichens were collected from 11 plots representing different vegetation types. From these collections, 126 lichen species are reported, 54 of which are new reports for St. Eustatius. Most species could be identified to species level based on morphological and chemical characters. In a few cases, mtSSU DNA sequences were generated for a preliminary molecular identification and future phylogenetic studies. In total, 263 identified lichen species are currently known from St. Eustatius, as well as some additional genera with yet unidentified species and lichenicolous fungi.

Keywords

Biodiversity inventory, lichens, mtSSU, St. Eustatius

Introduction

Sint Eustatius is a small island (21 km2) in the northern Leeward Islands part of the West Indies. It is one of the six islands of the Netherlands Antilles and, since 2010, a special municipality of the Netherlands. Sint Eustatius is roughly divided into three parts, the Northern Hills, the urbanised central area (‘Cultuurvlakte’) and the southern part dominated by the steep dormant volcano The Quill (600 m elev.). Although the whole island of St. Eustatius has been heavily impacted by human activities, the northern and southern parts are nowadays designated as National Parks with varied vegetation types especially on the slopes of The Quill. The latter comprise, for example, thorny woodlands, deciduous to evergreen seasonal forests, dry evergreen forest, montane thickets and elfin woodland (Stoffers 1956). A re-classification of the vegetation of St. Eustatius, based on cluster analysis of sample plots, resulted in 13 vegetation types characterised by different combinations of individual vascular plant species (Freitas et al. 2014).

Just as in almost all other groups of organisms, lichens are most diverse in the tropics (Sipman and Aptroot 2001). On St. Eustatius, they are commonly present on various substrates, including tree bark and twigs, siliceous rock, limestone, dead wood and living leaves. Despite their abundance, the lichen flora of St. Eustatius is still incompletely known. The authors are aware of only nine publications citing in total 14 lichen species from St. Eustatius, viz. Phyllopsora corallina (Eschw.) Müll. Arg. and P. parvifoliella (Nyl.) Müll. Arg. (Brako 1991), Anisomeridium excellens (Müll. Arg.) R.C. Harris (Harris 1995), Syncesia glyphysoides (Fée) Tehler (Tehler 1997), Pseudopyrenula subnudata Müll. Arg. (Harris 1998, as P. diluta var. degenerans Vain.), Stirtonia neotropica Aptroot, described based on material from St. Eustatius and Costa Rica (Aptroot 2009), Syncesia subintegra Sipman, described based on material from St. Eustatius, as well as S. farinacea (Fée) Tehler, S. glyphysoides and S. graphica (Fr.) Tehler (Sipman 2009), Roccella gracilis Bory (Aptroot and Schumm 2011), Dirina paradoxa (Fée) Tehler (Tehler et al. 2013), as well as Astrothelium bicolor (Taylor) Aptroot & Lücking, A. phlyctaena (Fée) Aptroot & Lücking and Pseudopyrenula subnudata Müll. Arg. (Aptroot and Lücking 2016).

The main source of information about the lichen flora of St. Eustatius is the online portal ‘Plants and Lichens of St. Eustatius’ (Boom et al. 2009). It contains a list with 209 lichen species, based on specimens collected by H. Sipman and W.R. Buck in 2008, identified by H. Sipman and mostly hosted in B (some in NY). In addition, some identified specimens are present in various other herbaria, some of which can be searched online. For instance, the database of BR cites the following identified specimens from St. Eustatius, collected by R. Hensen in 1991 and identified by the first author: Megalaria bengalensis Jagadeesh Ram & Aptroot, Porina mastoidea (Ach.) Müll. Arg. and Sticta xanthotropa (Kremp.) D.J. Galloway.

In 2015, a plot-based, multi-taxon biodiversity inventory of St. Eustatius was carried out by Naturalis Biodiversity Center, the European Invertebrate Survey (EIS) and different Dutch non-governmental organisations, together with St. Eustatius National Parks Foundation (STENAPA) and students from different Dutch universities. Here, the lichen records of that inventory are reported and an updated checklist of the lichens known from St. Eustatius is presented.

Materials and methods

As part of a multi-taxon inventory, lichens were collected on St. Eustatius from 11 plots (25 m × 25 m) in different main vegetation types according to Freitas et al. (2014). Two plots (H1, H2) were situated in the Northern Hills area, eight (M1−M5, M7−M9) on and around The Quill in the southern part of the island and one (U1) in the central urban area. Details concerning the location and vegetation characteristics of the plot locations and the multi-taxon sampling approach are described in van Andel et al. (2016). Lichens were collected using a knife or hammer and chisel and subsequently air-dried and stored in paper bags.

Specimens were observed and identified by the first author using an Olympus SZX7 stereomicroscope and an Olympus BX50 compound microscope with interference contrast, connected to a Nikon Coolpix digital camera. Sections were mounted in tap water, in which all measurements were also taken. The chemistry of selected specimens was investigated by thin-layer chromatography (Orange et al. 2001), using solvent A.

DNA analysis based on mitochondrial ribosomal small subunit (mtSSU) sequences was carried out for ten unidentifiable or provisionally identified specimens of good quality (indicated in Table 1). Although the nuclear ribosomal ITS region is the generally accepted fungal DNA barcode locus (Schoch et al. 2012), mtSSU was chosen since more mtSSU than ITS sequences have yet been published for several of the genera or families to which the respective specimens putatively belong.

Genomic DNA was extracted using the NucleoMag 96 Plant kit (Macherey-Nagel) on the KingFisher Flex Purification System (ThermoFisher Scientific). The mtSSU region was PCR-amplified following Zoller et al. (1999) in terms of primers (mrSSU1/mrSSU3R) and the PCR protocol. PCR products were purified and sequenced at BaseClear B.V. (www.baseclear.com) using the amplification primers. Sequences were assembled and edited using Geneious v8.1.8 (Biomatters Ltd.) and subjected to a BLAST search (Altschul et al. 1990) against the GenBank database (megablast; considering, where possible, BLAST results with E value of 0.0 and query cover >90 %). Sequences are available in GenBank under accession numbers MH028639MH028646.

To compile an updated list of the lichens of St. Eustatius, literature and internet sources were exhaustively consulted for previous reports and previous collectors were contacted for additional information.

Lichenised and lichenicolous fungi recorded in 11 plots on St. Eustatius. Species names in earlier publications are indicated in brackets. Author names are given in Table 2. Plots H1 and H2 are situated in the Northern Hills, M2−M9 on and around the volcano The Quill in the southern part of the island and U1 in the central urban area. Substrates occupied by each taxon are indicated per plot; b: bark, k: limestone, l: leaves, r: siliceous rock, s: soil, w: wood. Asterisks indicate first records for St. Eustatius (asterisks in brackets indicate additional taxa that are not yet identified to species level). Black dots (•) indicate specimens from which DNA was extracted.

Taxon H1 H2 M1 M2 M3 M4 M5 M7 M8 M9 U1
(*)Acanthothecis sp. • b
Alyxoria culmigena (Opegrapha herbarum) r w b
*Alyxoria varia b b b b
*Amandinea multispora b
*Anisomeridium subprostans b
Anisomeridium tamarindi b
(*)Anisomeridium sp. corticate c. pycnidia • b
*Anisomeridium terminatum b
Arthonia antillarum b b
Arthonia caribaea b
Arthonia conferta b b b b b b b
Arthonia cyrtodes b
Arthonia minuta b b
*Arthonia parantillarum b b
Arthothelium macrothecum b b
*Bacidia medialis b, r r
(*)Bacidia sp. apotheciate • b, w
(*)Bacidia sp. sorediate • b
Bactrospora denticulata b b b b b
*Bactrospora jenikii b
*Bogoriella annonacea b b
*Brigantiaea leucoxantha b
Buellia dejungens r r r r r
*Buellia griseovirens w w
Buellia mamillana (Buellia glaziouana) r
Caloplaca leptozona r r
*Caloplaca obscurella w
Coenogonium linkii b b
*Coenogonium saepincola w
Coenogonium strigosum b
Coniocarpon cinnabarinum (Arthonia cinnabarina) b
*Crespoa carneopruinata b, r
*Cresponea flava r
*Cryptothecia punctosorediata b
Cryptothecia striata b b, r
(*)Cryptothecia sp. isidiate • b
(*)Cryptothecia sp. sterile • b
*Dactylospora saxatilis (lichenicolous on Pertusaria praetervisa) r
Dichosporidium nigrocinctum b, r b, r
*Dictyomeridium amylosporum b b
Diorygma hieroglyphicum b
Diorygma poitaei b
*Diorygma pruinosum b
*Endocarpon pallidulum r r r, k r, s r
Enterographa pallidella b b
Enterographa subserialis b
Flakea papillata b b, r, w b, r r
Glyphis scyphulifera b
Graphis caesiella b
*Graphis cincta b
Graphis dendrogramma b b
*Graphis librata b
Gyalolechia bassiae (Caloplaca bassiae) b b, r
*Hafellia curatellae b
Hyperphyscia adglutinata b b, r b
Lathagrium neglectum (Collema neglectum) b
Lecanora legalloana r r r r r
*Lecanora leproplaca b b b
Lecanora leprosa r
Lecanora prosecha r r r r r r
Lecanora sulfurescens r
*Lepraria finkii b
*Leprocollema nova-caledonianum w
Letrouitia domingensis b
Malmidea piperis (Malcolmiella piperis) b
*Malmidea psychotrioides b
Malmidea vinosa (Malcolmiella vinosa) b
Mazosia carnea (Mazosia ocellata) b b
(*)Melaspilea sp. (lichenicolous on Pyrenula dissimulans) b
*Microtheliopsis uleana l
*Mycoporum eschweileri b b b b b
Nyungwea anguinella (Enterographa anguinella) b
*Opegrapha astraea b b
*Opegrapha lithyrgiza r
*Opegrapha quintana b
(*)Opegrapha sp. k
Peltula bolanderi r
Peltula obscurans r r r
Pertusaria coccopoda r r
Pertusaria praetervisa r r
*Pertusaria texana b
Pertusaria xanthodes b
*Phaeographis crispata b
Phaeographis dendritica b
*Phyllopeltula corticola b
Phyllopsora corallina b b, r r
Physcia atrostriata b
*Physcia erumpens b
*Physcia integrata b b
Physcia sorediosa b
*Porina conspersa b, r r
Porina epiphylla l
Porina internigrans b b
*Porina leptalea r
Porina nucula b, r
*Porina rubentior l
Porina tetracerae b b r
*Porina thaxteri l
(*)Psorotichia cf. americana r
*Pyrenopsis antillarum r
*Pyrenula adacta b b b b
Pyrenula breutelii (Pyrenula macularis) b b
Pyrenula cocoes b b b b b
*Pyrenula cruenta b
*Pyrenula dissimulans b b b b b
Pyrenula nitidula b
Pyxine cocoes b, r r r r b
*Ramalina stoffersii r
*Rinodina antillarum r
*Rinodina colobinoides b b
Rinodina pyxinoides r r r r r r
Sarcographa cf. tricosa b
Squamulea subsoluta (Caloplaca subsoluta) r r r r
*Staurolemma dussii b
*Sticta xanthotropa r r
*Stigmatochroma gerontoides w
(*)Stigmidium cf. schaereri k
*Strigula decipiens r
*Strigula phaea b, r
Strigula smaragdula l
*Syncesia decussans b
*Thelenella luridella r r r
(*)Thelidium cf. decipiens k
(*)Verrucaria cf. dolosa r
*Verrucaria nigrescens r
(*)Wetmoreana cf. appressa r r

Results and discussion

In total, 126 lichen species (and one identifiable lichenicolous fungus) were found in 243 collections (Table 1). The vast majority (113 species) could be identified to species level based on morphological and chemical characters, even though no identification book exists for any region nearby. However, many species have been described from other islands in the Caribbean, which can be expected to have many species in common. These were often already described in the 19th century and partly never studied again, but illustrations of their types are increasingly available. The authors also had access to various unpublished sources, such as the unpublished keys, descriptions and specimen citation (by H. Sipman) that was the basis of the internet checklist of St. Eustatius lichens and keys to the lichens from Puerto Rico (Harris 1989) and Guadeloupe (Øvstedal 2010), the latter with many illustrations of type and other specimens.

Somewhat to the authors’ surprise, as many as 54 (almost 50 %) of the identified species are new records for St. Eustatius. This includes mostly relatively common and widespread tropical or Neotropical species, but also some rare species, notably Staurolemma dussii (Vain.) P.M. Jørg. & Henssen, which was so far only known from its type from Guadeloupe. Furthermore, it is remarkable that Cresponea flava (Vain.) Egea & Torrente was found on siliceous rock. The presence of so many additional species within the limited surface area of the plots, totalling 6875 m2 (0.03% of the total island surface), suggests that the exploration of the lichen flora of St. Eustatius has not yet been exhaustive. However, no clearly undescribed species were found in the material and the number of species described based on material from St. Eustatius remains low with two, viz. Stirtonia neotropica (Aptroot 2009) and Syncesia subintegra (Sipman 2009).

Several specimens could not be identified with certainty in the present material but represent additional species (and in several cases additional genera). These are, for instance, Lichinaceae and Verrucariaceae, of which the taxonomy of the tropical taxa is incompletely known. Rather than describing them as new, they were listed with the name of the species that is morphologically most similar, preceded by “cf”. The BLAST results from the mtSSU sequences obtained from eight of these specimens in most cases allowed preliminary insights into their phylogenetic position.

The sequence of the Anisomeridium specimen with only conidia from St. Eustatius receives the highest BLAST hits with other representatives of the Monoblastiaceae in Nelsen et al. (2009, 2011), viz. Anisomeridium ubianum (Vain.) R.C. Harris, A. cf. willeyanum (R.C. Harris) R.C. Harris, Megalotremis verrucosa (Makhija & Patw.) Aptroot and Trypetheliopsis kalbii (Lücking & Sérus.) Aptroot. The low sequence identities of 86−93% clearly indicate that the St. Eustatius specimen belongs to another species in that family, but too few mtSSU sequences are yet available for a more precise molecular identification.

In the Graphidaceae, the top five BLAST hits for the specimen of Acanthothecis sp. were all with Acanthothecis peplophora (M. Wirth & Hale) E. Tripp & Lendemer specimens (97% identity), whereas the identity with the sequence of the type species of Acanthothecis, A. hololeucoides (Nyl.) Staiger & Kalb, was only 89%. The specimen from St. Eustatius thus most probably does not belong to Acanthothecis s.str., but may represent a species of ‘Acanthothecis 2’ in the Carbacanthographis clade (cf. Rivas Plata et al. 2013, Medeiros et al. 2017). The Sarcographa cf. tricosa specimen received BLAST hits of 97% identity with Sarcographina glyphiza (Nyl.) Kr.P. Singh & D.D. Awasthi and Pallidogramme chlorocarpoides (Nyl.) Staiger, Kalb & Lücking, both situated in the Graphioideae tribe Graphidae p.p. clade of Rivas Plata et al. (2013). However, another GenBank sequence of P. chlorocarpoides, as well as several species of other genera of same clade, were 96 % identical, including the single other specimen of S. tricosa in GenBank (but not the species of the Sarcographa s.str. clade sensu Rivas Plata et al. 2013). The identity of the St. Eustatius specimen thus remains ambiguous based on the presently available mtSSU sequence data.

Both the apotheciate and sorediate Bacidia specimens are closest to sequences of species of the Toninia-Bacidia p.p. clade in Miadlikowska et al. (2014), the former to Toninia sedifolia (Scop.) Timdal (94 % identity) and the latter to Bacidia californica S. Ekman and B. phacodes Körb. (88−89 % identity), respectively. Consequently, they do not belong to Bacidia s.str., which forms a separate clade (including the type species, B. rosella (Pers.) De Not.) in Miadlikowska et al. (2014).

In the Verrucariaceae, Verrucaria was resolved as polyphyletic and Thelidium mixed with Polyblastia, Staurothele p.p. and Verrucaria p.p. (Polyblastia clade) in molecular phylogenetic reconstructions (Gueidan et al. 2007, Muggia et al. 2010, Thüs et al. 2011). The sequence of the Thelidium cf. decipiens specimen from St. Eustatius, however, is closest to the Catapyrenium-Placidiopsis-Verrucaria p.p. (V. caerulea DC., V. praetermissa (Trevis.) Anzi) clade (Muggia et al. 2010) with sequence identities of 96−97 %. The placement of the Verrucaria cf. dolosa specimen is more difficult to assess, since its sequence shows lower similarities of 92−94 % to representatives of different Verrucariceae genera, such as Agonimia, Bagliettoa and Verrucaria spp.

Finally, the mtSSU sequence of the Wetmoreana cf. appressa specimen from St. Eustatius is difficult to interpret, since it matches more closely with sequences of the Xanthorioideae (sequence identity 97−99 %) than with Teloschistoideae, in which Wetmoreana is placed (e.g. Arup et al. 2013).

The lichen flora of St. Eustatius can be characterised as lowland, relatively dry Caribbean. As can be seen from Table 1, most species were found on one substratum type, but some are less specialised. Also, there is a marked difference between the lichens of the different plots and the three main areas on St. Eustatius (Northern Hills, central urban area, The Quill). However, the authors refrain from performing statistical comparisons of the lichen diversity between plots, since the number of plots per main area differs and is still low and the sampling strategy was devised by specialists of other organism groups. Nevertheless, the lichen data will be useful for an island-wide, plot-based comparison of diversity amongst all organism groups sampled during the 2015 inventory.

In Table 2, an updated checklist is presented of the lichens of St. Eustatius, citing only identified species, but based on all available sources and with their taxonomy (nomenclature and sometimes species concept) updated where necessary. According to this list, a total of 263 species are currently known from St. Eustatius. As a side effect of revising the existing records, one record becomes questionable, viz. Myriostigma candidum Kremp., which is not known from the Neotropics. It is intended to continue the exploration of the lichens of this island in the near future.

Updated checklist of the lichens of St. Eustatius.

Species References
Lichens
Acarospora chrysops (Tuck.) H.Magn. Boom et al. (2009) as Acarospora dissipata H.Magn.
Alyxoria culmigena (Lib.) Ertz Boom et al. (2009) as Opegrapha herbarum Mont., present study
Alyxoria ochrocheila (Nyl.) Ertz & Tehler Boom et al. (2009) as Opegrapha ochrocheila Nyl.
Alyxoria varia (Pers.) Ertz & Tehler present study
Amandinea efflorescens (Müll. Arg.) Marbach Boom et al. (2009)
Amandinea multispora (Kalb & Vězda) Marbach present study
Amandinea prospersa (Nyl.) Elix & H. Mayhofer Boom et al. (2009) as Buellia prospersa (Nyl.) Riddle
Anisomeridium americanum (A.Massal.) R.C. Harris Boom et al. (2009)
Anisomeridium excellens (Müll. Arg.) R.C. Harris Boom et al. (2009), Harris (1995)
Anisomeridium subprostans (Nyl.) R.C. Harris present study
Anisomeridium tamarindi (Fée) R.C. Harris Boom et al. (2009), present study
Anisomeridium terminatum (Nyl.) R.C. Harris present study
Anisomeridium tuckerae (R.C. Harris) R.C. Harris Boom et al. (2009)
Arthonia antillarum (Fée) Nyl. Boom et al. (2009), present study
Arthonia caribaea (Ach.) A. Massal. Boom et al. (2009), present study
Arthonia conferta (Fée) Nyl. Boom et al. (2009), present study
Arthonia cyanea Müll. Arg. Boom et al. (2009)
Arthonia cyrtodes Nyl. Boom et al. (2009), present study
Arthonia minuta Vain. Boom et al. (2009), present study
Arthonia parantillarum Aptroot present study
Arthothelium macrothecum (Fée) A. Massal. Boom et al. (2009), present study
Astrothelium bicolor (Taylor) Aptroot & Lücking Boom et al. (2009) as Trypethelium nitidiusculum (Nyl.) R.C. Harris, Aptroot & Lücking (2016)
Astrothelium phlyctaena (Fée) Aptroot & Lücking Boom et al. (2009) as Trypethelium ochroleucum (Eschw.) Nyl., Aptroot & Lücking (2016)
Bacidia medialis (Tuck.) Zahlbr. present study
Bactrospora denticulata (Vain.) Egea & Torrente Boom et al. (2009), present study
Bactrospora jenikii (Vězda) Egea & Torrente present study
Bactrospora myriadea (Fée) Egea & Torrente Boom et al. (2009)
Baculifera intermedioides Marbach Boom et al. (2009)
Blastenia brittonii Zahlbr. Boom et al. (2009) as Caloplaca brittonii (Zahlbr.) ined.
Bogoriella annonacea (Müll. Arg.) Aptroot & Lücking present study
Brigantiaea leucoxantha (Spreng.) R. Sant. & Hafellner present study
Brownliella cinnabarina (Ach.) S.Y. Kondr., Kärnefelt, A. Thell, Elix, J.Kim, A.S.Kondr. & J.-S.Hur Boom et al. (2009) as Caloplaca cinnabarina (Ach.) Zahlbr.
Buellia boergesenii Imshaug Boom et al. (2009)
Buellia dejungens (Nyl.) Vain. Boom et al. (2009), present study
Buellia griseovirens (Turner & Borrer ex Sm.) Almb. present study
Buellia mamillana (Tuck.) W.A. Weber Boom et al. (2009) as Buellia glaziouana (Kremp.) Müll. Arg., present study
Buellia posthabita (Nyl.) Zahlbr. Boom et al. (2009)
Bulbothrix scortella (Nyl.) Hale Boom et al. (2009)
Bulbothrix suffixa (Stirt.) Hale Boom et al. (2009)
Byssoloma leucoblepharum (Nyl.) Vain. Boom et al. (2009)
Caloplaca diplacia (Ach.) Riddle Boom et al. (2009)
Caloplaca leptozona (Nyl.) Zahlbr. Boom et al. (2009), present study
Caloplaca obscurella (J. Lahm) Th. Fr. present study
Canoparmelia martinicana (Nyl.) Elix & Hale Boom et al. (2009)
Carbacanthographis triphoroides (M. Wirth & Hale) Lücking Boom et al. (2009)
Chapsa cinchonarum (Fée) Frisch Boom et al. (2009)
Chrysothrix xanthina (Vain.) Kalb Boom et al. (2009)
Cladonia corymbites Nyl. Boom et al. (2009)
Cladonia didyma (Fée) Vain. Boom et al. (2009)
Cladonia subradiata (Vain.) Sandst. Boom et al. (2009)
Coccocarpia palmicola (Spreng.) Arv. & D.J. Galloway Boom et al. (2009)
Coccocarpia pellita (Ach.) Müll. Arg. Boom et al. (2009)
Coenogonium interpositum Nyl. Boom et al. (2009)
Coenogonium leprieurii (Mont.) Nyl. Boom et al. (2009)
Coenogonium linkii Ehrenb. Boom et al. (2009), present study
Coenogonium saepincola Aptroot, Sipman & Lücking present study
Coenogonium strigosum Rivas Plata, Lücking & Chaves Boom et al. (2009), present study
Coenogonium subdilutum (Malme) Lücking, Aptroot & Sipman Boom et al. (2009)
Coniocarpon cinnabarinum DC. Boom et al. (2009) as Arthonia cinnabarina (DC.) Wallr., present study
Cratiria lauricassiae (Fée) Marbach Boom et al. (2009)
Crespoa carneopruinata (Zahlbr.) Lendemer & B.P. Hodk. present study
Cresponea flava (Vain.) Egea & Torrente present study
Cresponea leprieurii (Mont.) Egea & Torrente Boom et al. (2009)
Cresponea proximata (Nyl.) Egea & Torrente Boom et al. (2009)
Cryptolechia carneolutea (Tuck.) A. Massal. Boom et al. (2009)
Cryptothecia megalocarpa (Müll. Arg.) R. Sant. Boom et al. (2009)
Cryptothecia punctosorediata Sparrius present study
Cryptothecia striata G. Thor Boom et al. (2009), present study
Dichosporidium nigrocinctum (Ehrenb.) G. Thor Boom et al. (2009), present study
Dictyomeridium amylosporum (Vain.) Aptroot, M.P. Nelsen & Lücking present study
Diorygma hieroglyphicum (Pers.) Staiger & Kalb Boom et al. (2009), present study
Diorygma poitiaei (Fée) Kalb, Staiger & Elix Boom et al. (2009), present study
Diorygma pruinosum (Eschw.) Kalb, Staiger & Elix present study
Diploschistes actinostomus (Ach.) Zahlbr. Boom et al. (2009)
Diploschistes aeneus (Müll. Arg.) Lumbsch Boom et al. (2009)
Diploschistes prominens (Vain.) Lumbsch Boom et al. (2009)
Dirina paradoxa (Fée) Tehler Boom et al. (2009) as Dirina approximata subsp. hioramii (B. de Lesd.) Tehler, Tehler et al. (2013)
Dirinaria aegialita (Ach.) B.J. Moore Boom et al. (2009)
Endocarpon pallidulum (Nyl.) Nyl. present study
Enterographa compunctula (Nyl.) Redinger Boom et al. (2009)
Enterographa multilocularis (Müll. Arg.) Sparrius Boom et al. (2009)
Enterographa pallidella (Nyl.) Redinger Boom et al. (2009), present study
Enterographa perez-higaredae Herrera-Camp. & Lücking Boom et al. (2009)
Enterographa sipmanii Sparrius Boom et al. (2009)
Enterographa subserialis (Nyl.) Redinger Boom et al. (2009), present study
Eremothecella microcephalica Sipman Boom et al. (2009)
Fellhanera santessonii Barillas & Lücking Boom et al. (2009)
Fissurina dumastii Fée Boom et al. (2009)
Flakea papillata O.E. Erikss. Boom et al. (2009), present study
Glyphis cicatricosa Ach. Boom et al. (2009)
Glyphis scyphulifera (Ach.) Staiger Boom et al. (2009), present study
Graphis caesiella Vain. Boom et al. (2009), present study
Graphis chondroplaca (Redinger) Lücking Boom et al. (2009)
Graphis cincta (Pers.) Aptroot present study
Graphis dendrogramma Nyl. Boom et al. (2009), present study
Graphis furcata Fée Boom et al. (2009)
Graphis glaucescens Fée Boom et al. (2009)
Graphis librata C. Knight present study
Graphis lineola Ach. Boom et al. (2009)
Graphis tenella Ach. Boom et al. (2009)
Graphis tenellula Vain. Boom et al. (2009)
Gyalectidium filicinum Müll. Arg. Boom et al. (2009)
Gyalolechia bassiae (Ach.) Søchting, Frödén & Arup ex Ahti Boom et al. (2009) as Caloplaca bassiae (Ach.) Zahlbr., present study
Hafellia bahiana (Malme) Sheard Boom et al. (2009)
Hafellia curatellae (Malme) Marbach present study
Herpothallon aurantiacoflavum (B. de Lesd.) Aptroot, Lücking & G.Thor Boom et al. (2009)
Heterodermia albicans (Pers.) Swinscow & Krog Boom et al. (2009)
Heterodermia galactophylla (Tuck.) W.L. Culb. Boom et al. (2009)
Heterodermia lutescens (Kurok.) Follmann Boom et al. (2009)
Heterodermia obscurata (Nyl.) Trevis. Boom et al. (2009)
Heterodermia squamulosa (Degel.) W.L. Culb. Boom et al. (2009)
Heterodermia verrucifera (Kurok.) W.A. Weber Boom et al. (2009)
Hyperphyscia adglutinata (Flörke) H. Mayrhofer & Poelt Boom et al. (2009), present study
Hyperphyscia minor (Fée) D.D. Awasthi Boom et al. (2009)
Lathagrium neglectum (Degel.) Otálora, P.M. Jørg. & Wedin Boom et al. (2009) as Collema neglectum Degel., present study
Lecanactis epileuca (Nyl.) Tehler Boom et al. (2009)
Lecanora galactiniza Nyl. Boom et al. (2009)
Lecanora legalloana Elix & Øvstedal Boom et al. (2009), present study
Lecanora leproplaca Zahlbr. present study
Lecanora leprosa Fée Boom et al. (2009), present study
Lecanora prosecha Ach. Boom et al. (2009), present study
Lecanora sulfurescens Fée Boom et al. (2009), present study
Leiorreuma exaltatum (Mont. & Bosch) Staiger Boom et al. (2009)
Lepraria finkii (B. de Lesd.) R.C. Harris present study
Leprocollema novacaledonianum A.L. Sm. present study
Leptogium austroamericanum (Malme) C.W. Dodge Boom et al. (2009)
Leptogium azureum (Sw.) Mont. Boom et al. (2009)
Leptogium cyanescens (Rabenh.) Körb. Boom et al. (2009)
Leptogium marginellum (Sw.) Gray Boom et al. (2009)
Letrouitia domingensis (Pers.) Hafellner & Bellem. Boom et al. (2009), present study
Leucodecton bisporum (Nyl.) Sipman & Lücking Boom et al. (2009)
Leucodecton compunctum (Ach.) A. Massal. Boom et al. (2009)
Loflammia gabrielis (Müll. Arg.) Vezda Boom et al. (2009)
Malmidea piperis (Spreng.) Kalb, Rivas Plata & Lumbsch Boom et al. (2009) as Malcolmiella piperis (Spreng.) Kalb & Lücking, present study
Malmidea psychotrioides (Kalb & Lücking) Kalb, Rivas Plata & Lumbsch present study
Malmidea vinosa (Eschw.) Kalb, Rivas Plata & Lumbsch Boom et al. (2009) as Malcolmiella vinosa (Eschw.) Kalb & Lücking, present study
Mazosia carnea (Eckfelt) Aptroot & M. Cáceres Boom et al. (2009) as Mazosia ocellata (Nyl.) R.C. Harris, present study
Mazosia phyllosema (Nyl.) Zahlbr. Boom et al. (2009)
Megalaria bengalensis Jagadeesh Ram & Aptroot Hensen (BR)
Melanotrema meiospermum (Nyl.) Frisch Boom et al. (2009)
Microtheliopsis uleana Müll. Arg. present study
Mycoporum eschweileri (Müll. Arg.) R.C. Harris present study
Myriostigma candidum Kremp. Boom et al. (2009) as Cryptothecia candida (Kremp.) R. Sant.: incorrect report
Myriotrema myriotremoides (Nyl.) Hale Boom et al. (2009)
Myriotrema olivaceum Fée Boom et al. (2009)
Nyungwea anguinella (Nyl.) Aptroot Boom et al. (2009) as Enterographa anguinella (Nyl.) Redinger, present study
Ocellularia depressa (Mont.) Hale Boom et al. (2009)
Ocellularia interposita (Nyl.) Hale Boom et al. (2009)
Ocellularia terebrata (Ach.) Müll. Arg. Boom et al. (2009)
Opegrapha astraea Tuck. present study
Opegrapha lithyrgiza Vain. present study
Opegrapha quintana Redinger present study
Pannaria prolificans Vain. Boom et al. (2009)
Parmotrema crinitum (Ach.) M. Choisy Boom et al. (2009)
Parmotrema endosulphureum (Hillmann) Hale Boom et al. (2009)
Parmotrema praesorediosum (Nyl.) Hale Boom et al. (2009)
Parmotrema tinctorum (Nyl.) Hale Boom et al. (2009)
Parmotrema ultralucens (Krog) Hale Boom et al. (2009)
Peltula bolanderi (Tuck.) Wetmore Boom et al. (2009), present study
Peltula obscurans (Nyl.) Gyeln. Boom et al. (2009), present study
Pertusaria coccopoda Vain. Boom et al. (2009), present study
Pertusaria leioplacella Nyl. Boom et al. (2009)
Pertusaria praetervisa Vain. Boom et al. (2009), present study
Pertusaria texana Müll. Arg. present study
Pertusaria xanthodes Müll. Arg. Boom et al. (2009), present study
Phaeographis crispata Kalb & Matthes-Leicht present study
Phaeographis dendritica (Ach.) Müll. Arg. Boom et al. (2009), present study
Phaeographis intricans (Nyl.) Staiger Boom et al. (2009)
Phaeographis scalpturata (Ach.) Staiger Boom et al. (2009)
Phyllopeltula corticola (Büdel & R. Sant.) Kalb present study
Phyllopsora chlorophaea (Müll. Arg.) Zahlbr. Boom et al. (2009)
Phyllopsora corallina (Eschw.) Müll. Arg. Brako (1991), Boom et al. (2009), present study
Phyllopsora glaucescens Timdal Boom et al. (2009)
Phyllopsora parvifoliella (Nyl.) Müll. Arg. Brako (1991), Boom et al. (2009)
Physcia atrostriata Moberg Boom et al. (2009), present study
Physcia crispa Nyl. Boom et al. (2009)
Physcia erumpens Moberg present study
Physcia integrata Moberg present study
Physcia sinuosa Moberg Boom et al. (2009)
Physcia sorediosa (Vain.) Lynge Boom et al. (2009), present study
Physcia tenuis Moberg Boom et al. (2009)
Platythecium colliculosum (Mont.) Staiger Boom et al. (2009)
Platythecium leiogramma (Nyl.) Staiger Boom et al. (2009)
Polymeridium quinqueseptatum (Nyl.) R.C. Harris Boom et al. (2009)
Porina conspersa Malme present study
Porina epiphylla (Fée) Fée Boom et al. (2009), present study
Porina internigrans (Nyl.) Müll. Arg. Boom et al. (2009), present study
Porina leptalea (Durieu & Mont.) A.L. Sm. present study
Porina mastoidea (Ach.) Müll. Arg. Hensen (BR), Boom et al. (2009)
Porina nitidula Müll. Arg. Boom et al. (2009)
Porina nucula Ach. Boom et al. (2009), present study
Porina octomera (Müll. Arg.) F.Schill. Boom et al. (2009)
Porina rubentior (Stirt.) Müll. Arg. present study
Porina tetracerae (Ach.) Müll. Arg. Boom et al. (2009), present study
Porina thaxteri R. Sant. present study
Pseudochapsa dilatata (Müll. Arg.) Parnmen, Lücking & Lumbsch Boom et al. (2009) as Chapsa dilatata (Müll. Arg.) Kalb
Pseudopyrenula subgregaria Müll. Arg. Boom et al. (2009)
Pseudopyrenula subnudata Müll. Arg. Harris (1998) as Pseudopyrenula diluta (Fée) Müll. Arg. var. degenerans Vain, Boom et al. (2009) as Pseudopyrenula diluta, Aptroot & Lücking (2016)
Pyrenopsis antillarum Vain. present study
Pyrenula adacta Fée present study
Pyrenula astroidea (Fée) R.C. Harris Boom et al. (2009)
Pyrenula bahiana Malme Boom et al. (2009) as Pyrenula concatervans (Nyl.) R.C. Harris
Pyrenula breutelii (Müll. Arg.) Aptroot Boom et al. (2009) as Pyrenula macularis (Zahlbr.) R.C. Harris, present study
Pyrenula cinerea Zahlbr. Boom et al. (2009)
Pyrenula cocoes Müll. Arg. Boom et al. (2009), present study
Pyrenula confinis (Nyl.) R.C. Harris Boom et al. (2009)
Pyrenula cruenta (Mont.) Vain. present study
Pyrenula dissimulans (Müll. Arg.) R.C. Harris present study
Pyrenula duplicans (Nyl.) Aptroot Boom et al. (2009)
Pyrenula leucostoma Ach. Boom et al. (2009)
Pyrenula mamillana (Ach.) Trevis. Boom et al. (2009) as Pyrenula xyloides (Eschw.) Müll. Arg.
Pyrenula massariospora (Starbäck) R.C. Harris Boom et al. (2009)
Pyrenula microtheca R.C. Harris Boom et al. (2009)
Pyrenula nitidula (Bres.) R.C. Harris Boom et al. (2009), present study
Pyrenula septicollaris (Eschw.) R.C. Harris Boom et al. (2009)
Pyxine cocoes (Sw.) Nyl. Boom et al. (2009), present study
Ramalina anceps Nyl. Boom et al. (2009)
Ramalina complanata (Sw.) Ach. Boom et al. (2009)
Ramalina dendroides (Nyl.) Nyl. Boom et al. (2009)
Ramalina furcellata (Ach.) Zahlbr. Boom et al. (2009)
Ramalina stoffersii Sipman present study
Rinodina antillarum Vain. present study
Rinodina colobinoides (Nyl.) Müll. Arg. present study
Rinodina pyxinoides Vain. Boom et al. (2009), present study
Roccella gracilis Bory Boom et al. (2009), Aptroot and Schumm (2011)
Roccellographa circumscripta (Leight.) Ertz & Tehler Boom et al. (2009) as Peterjamesia circumscripta (Taylor) D. Hawksw.
Sarcographa heteroclita (Mont.) Zahlbr. Boom et al. (2009)
Sarcographa labyrinthica (Ach.) Müll. Arg. Boom et al. (2009)
Sarcographa ramificans (Kremp.) Staiger Boom et al. (2009)
Sarcographa tricosa (Ach.) Müll. Arg. Boom et al. (2009), present study
Sclerophyton elegans Eschw. Boom et al. (2009)
Sclerophyton trinidadense Sparrius Boom et al. (2009)
Sporopodium phyllocharis (Mont.) A. Massal. Boom et al. (2009)
Squamulea subsoluta (Nyl.) Arup, Søchting & Frödén Boom et al. (2009) as Caloplaca subsoluta (Nyl.) Zahlbr., present study
Staurolemma dussii (Vain.) P.M. Jørg. & Henssen present study
Stegobolus auberianus (Mont.) Frisch & Kalb Boom et al. (2009)
Stegobolus granulosus (Tuck.) Frisch Boom et al. (2009)
Stegobolus subcavatus (Nyl.) Frisch Boom et al. (2009)
Sticta xanthotropa (Kremp.) D.J. Galloway Hensen (BR), present study
Stigmatochroma gerontoides (Stirt.) Marbach present study
Stirtonia neotropica Aptroot Aptroot (2009)
Strigula decipiens (Malme) P.M. McCarthy present study
Strigula macrospora Vain. Boom et al. (2009)
Strigula nemathora Mont. Boom et al. (2009)
Strigula obducta (Müll. Arg.) R.C. Harris Boom et al. (2009)
Strigula phaea (Ach.) R.C. Harris present study
Strigula smaragdula Fr. Boom et al. (2009), present study
Synalissa lichinella Vain. Boom et al. (2009)
Syncesia decussans (Nyl.) Tehler present study
Syncesia farinacea (Fée) Tehler Boom et al. (2009), Sipman (2009)
Syncesia glyphysoides (Fée) Tehler Tehler (1997), Boom et al. (2009), Sipman (2009)
Syncesia graphica (Fr.) Tehler Boom et al. (2009), Sipman (2009)
Syncesia subintegra Sipman Boom et al. (2009), Sipman (2009)
Teloschistes flavicans (Sw.) Norman Boom et al. (2009) as Teloschistes flavicans var. crocea (Ach.) Müll. Arg.
Thalloloma hypoleptum (Nyl.) Staiger Boom et al. (2009)
Thelenella luridella (Nyl.) H. Mayrhofer present study
Thelotrema porinoides Mont. & Bosch Boom et al. (2009)
Toninia submexicana B. de Lesd. Boom et al. (2009)
Trapelia coarctata (Sm.) M. Choisy Boom et al. (2009)
Usnea baileyi (Stirt.) Zahlbr. Boom et al. (2009)
Varicellaria velata (Turner) I. Schmitt & Lumbsch Boom et al. (2009)
Verrucaria nigrescens Pers. present study
Xanthoparmelia succedans Elix & J. Johnst. Boom et al. (2009)
Lichenicolous fungus
Dactylospora saxatilis (Schaer.) Hafellner (lichenicolous on Pertusaria praetervisa) present study
Additional genera (species uncertain)
Acanthothecis sp. present study
Bacidina sp. present study
Melaspilea sp. (lichenicolous fungus) present study
Psorotichia sp. present study
Stigmidium sp. (lichenicolous fungus) present study
Thelidium sp. present study
Wetmoreana sp. present study

Acknowledgements

This research was funded by Naturalis Biodiversity Center. Fieldwork was co-organized by T. van Andel, B. van der Hoorn and J. Miller (Naturalis) and facilitated by the Caribbean Netherlands Science Institute (CNSI), St. Eustatius National Parks (STENAPA), DCNA Island Conservationists H. Madden and A. van Zanten and field guide C. Gibbs. Students E. Haber, T. Huijts, C. Posthouwer, T. Verheijden, R. Vogel and S. Zwartsenberg of the Leiden University course Tropical Biodiversity and Field Methods as well as R. Butôt (Naturalis) contributed to collecting. Student participation was facilitated by the Alberta Mennega Stichting and the Van Eeden Fonds. Koos Biesmeijer facilitated the Naturalis Caribbean programme and participated in the fieldwork. Marlon Murray and the St. Eustatius Historical Foundation allowed us to sample the urban plot. Molecular lab work was carried out by R. Butôt. We are indebted to H. Sipman (Berlin) for providing a copy of the unpublished keys, descriptions and specimen citation that was the basis for the internet checklist by Boom et al. (2009).

References

  • Andel T van, Hoorn B van der, Stech M, Bantjes Arostegui S, Miller J (2016) A quantitative assessment of the vegetation types on the island of St. Eustatius, Dutch Caribbean. Global Ecology and Conservation 7: 59–69. https://doi.org/10.1016/j.gecco.2016.05.003
  • Aptroot A, Schumm F (2011) Fruticose Roccellaceae, an anatomical-microscopical atlas and guide with a worldwide key and further notes on some crustose Roccellaceae or similar lichens. Books on demand, Norderstedt.
  • Brako L (1991) Phyllopsora (Bacidiaceae). Flora Neotropica Monograph 55: 1‒66.
  • Freitas JA de, Rojer AC, Nijhof BSJ, Debrot AO (2014) A landscape ecological vegetation map of Sint Eustatius (Lesser Antilles). Den Helder.
  • Gueidan C, Roux C, Lutzoni F (2007) Using a multigene phylogenetic analysis to assess generic delineation and character evolution in Verrucariaceae (Verrucariales, Ascomycota). Mycological Research 111: 1145‒1168. https://doi.org/10.1016/j.mycres.2007.08.010
  • Harris RC (1989) Working keys to the lichen forming fungi of Puerto Rico. Tropical lichen workshop, Presented at Catholic University of Puerto Rico. [Published and distributed by the author]
  • Harris RC (1995) More Florida lichens. [Published and distributed by the author]
  • Harris RC (1998) A preliminary revision of Pseudopyrenula Müll. Arg. (lichenized Ascomycetes, Trypetheliaceae) with a redisposition of the names previously assigned to the genus. In: Glenn MG, Harris RC, Dirig R, Cole MS (Eds) Lichenographia Thomsoniana: North American Lichenology in Honor of John W. Thomson. Mycotaxon Ltd., Ithaca, New York, 133‒148.
  • Medeiros ID, Kraichak E, Lücking R, Mangold A, Lumbsch HT (2017) Assembling a Taxonomic Monograph of Tribe Wirthiotremateae (Lichenized Ascomycota: Ostropales: Graphidaceae). Fieldiana Life and Earth Sciences 9: 1‒31. https://doi.org/10.3158/2158-5520-16.9.1
  • Miadlikowska J, Kauff F, Högnabba F, Oliver JC, Molnár K, Fraker E, Gaya E, Hafellner J, Hofstetter V, Gueidan C, Otálora MAG, Hodkinson B, Kukwa M, Lücking R, Björk C, Sipman HJM, Burgaz AR, Thell A, Passo A, Myllys L, Goward T, Fernández-Brime S, Hestmark G, Lendemer J, Lumbsch HT, Schmull M, Schoch CL, Sérusiaux E, Maddison DR, Arnold AE, Lutzoni F, Stenroos S (2014) A multigene phylogenetic synthesis for the class Lecanoromycetes (Ascomycota): 1307 fungi representing 1139 infrageneric taxa, 317 genera and 66 families. Molecular Phylogenetics and Evolution 79: 132–168. https://doi.org/10.1016/j.ympev.2014.04.003
  • Muggia L, Gueidan C, Grube M (2010) Phylogenetic placement of some morphologically unusual members of Verrucariales. Mycologia 102: 835–846. https://doi.org/10.3852/09-153
  • Nelsen MP, Lücking R, Grube M, Mbatchou JS, Muggia L, Rivas Plata E, Lumbsch HT (2009) Unravelling the phylogenetic relationships of lichenised fungi in Dothideomyceta. Studies in Mycology 64: 135–144. https://doi.org/10.3114/sim.2009.64.07
  • Nelsen MP, Lücking R, Mbatchou JS, Andrew CJ, Spielmann AA, Lumbsch HT (2011) New insights into relationships of lichen-forming Dothideomycetes. Fungal Diversity 51: 155–162. https://doi.org/10.1007/s13225-011-0144-7
  • Orange A, James PW, White FJ (2001) Microchemical Methods for the Identification of Lichens. British Lichen Society, London.
  • Øvstedal DO (2010) The lichens of Guadeloupe, French Antilles. Bergen Museum, Bergen.
  • Rivas Plata E, Parnmen S, Staiger B, Mangold A, Frisch A, Weerakoon G, Hernández MJE, Cáceres MES, Kalb K, Sipman HJM, Common RS, Nelsen MP, Lücking R, Lumbsch HT (2013) A molecular phylogeny of Graphidaceae (Ascomycota, Lecanoromycetes, Ostropales) including 428 species. In: Boonpragob K, Crittenden P, Lumbsch HT (Eds) Lichens: from genome to ecosystems in a changing world. MycoKeys 6: 55–94. https://doi.org/10.3897/mycokeys.6.3482
  • Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences of the USA 109: 6241–6246. https://doi.org/10.1073/pnas.1117018109
  • Sipman HJM, Aptroot A (2001) Where are the missing lichens? Mycological Research 105: 1433‒1439.
  • Stoffers AL (1956) The vegetation of the Netherlands Antilles. Mededelingen van het Botanisch Museum van het Herbarium van de Rijksuniversiteit Utrecht 135: 1‒142. [+ 28 pl]
  • Tehler A (1997) Syncesia (Arthoniales, Euascomycetidae). Flora Neotropica Monograph 74: 1‒48.
  • Thüs H, Muggia L, Pérez-Ortega S, Favero-Longo SE, Joneson S, O’Brien H, Nelsen MP, Duque-Thüs R, Grube M, Friedl T, Brodie J, Andrew CJ, Lücking R, Lutzoni F, Gueidan C (2011) Revisiting photobiont diversity in the lichen family Verrucariaceae (Ascomycota). European Journal of Phycology 46: 399–415. https://doi.org/10.1080/09670262.2011.629788
  • Zoller S, Scheidegger C, Sperisen C (1999) PCR primers for the amplification of mitrochondrial small subunit ribosomal DNA of lichen-forming Ascomycetes. The Lichenologist 31: 511‒516. https://doi.org/10.1006/lich.1999.0220