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Research Article
Morphological and phylogenetic analyses reveal two new species of Camporesiomyces (Tubeufiaceae, Tubeufiales) from terrestrial habitats in China
expand article infoSong Bai‡, Fang Wang‡, Su-Ran Wan‡, Xiao-Kang Lv‡, Li-Jun Chen‡, Rong Wu‡, Jian Ma‡§
‡ Guizhou Industry Polytechnic College, Guiyang, China
§ School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang, China

Corresponding author: Li-Jun Chen ( clj102128@163.com )

Corresponding author: Jian Ma ( yanmajian@163.com )

Academic editor: Nattawut Boonyuen
© 2025 Song Bai, Fang Wang, Su-Ran Wan, Xiao-Kang Lv, Li-Jun Chen, Rong Wu, Jian Ma.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Bai S, Wang F, Wan S-R, Lv X-K, Chen L-J, Wu R, Ma J (2025) Morphological and phylogenetic analyses reveal two new species of Camporesiomyces (Tubeufiaceae, Tubeufiales) from terrestrial habitats in China. MycoKeys 124: 177-192. https://doi.org/10.3897/mycokeys.124.168385
Open Access

Abstract

During a survey of saprobic fungi in karst landscapes of Guizhou Province, China, fresh specimens were collected from decaying wood in terrestrial habitats. Phylogenetic analyses of a combined dataset (ITS, LSU, tef1-α and rpb2) along with morphological characteristics support the introduction of two novel species, Camporesiomyces qizhifengensis and C. yusheensis. Detailed descriptions, illustrations and phylogenetic evidence are provided to substantiate their taxonomic placement. Additionally, a checklist of currently accepted Camporesiomyces species supported by molecular data is included. This is the first report of Camporesiomyces in Guizhou, adding to the regional biodiversity of karst habitats and highlighting the ecological importance of these unique environments. The findings underscore the need for continued exploration of fungal diversity in underexplored regions.

Key words:

Asexual morph, Dothideomycetes, phylogeny, saprobic fungi, taxonomy, two new species

Introduction

Karst landscapes are primarily shaped by the dissolution of soluble rocks, such as limestone, dolomite and gypsum, through the action of groundwater or surface water. These landscapes span the provinces of Chongqing, Guangxi, Guizhou and Yunnan in China (Yang et al. 2023; Ma et al. 2024a, 2024b; Wang et al. 2024a, 2024b). Amongst them, Guizhou Province, with 62% of its land area (109,100 km2) covered by karst landscapes, is known for its rich biodiversity (Liu et al. 2018, 2020; Yang et al. 2023). Previous studies have explored the diversity, taxonomy and phylogeny of fungi in karst regions, primarily focusing on the development of freshwater fungal communities, with limited reports on the discovery of terrestrial fungi (Ma et al. 2022, 2023a, 2023b, 2023c, 2024a, 2024b; Calabon et al. 2022, 2023; Xiao et al. 2023; Xu et al. 2025).

Camporesiomyces was established by Hyde et al. (2020) to accommodate the type species, C. mali, along with two new combinations (C. patagonicus and C. vaccinii), based on morphological comparisons and phylogenetic analyses of combined ITS, LSU, tef1-α and rpb2 sequences. Previously, Camporesiomyces patagonicus and C. vaccinii were classified as Acanthostigma patagonicum and Helicoma vaccinii, respectively (Carris 1989; Sánchez et al. 2012; Hyde et al. 2020). Amongst them, Camporesiomyces vaccinii (≡ Helicoma vaccinii) was initially described by Carris (1989), with subsequent records from Peru and China in terrestrial habitats by Matsushima (1993) and Zhao et al. (2007), respectively. Tsui and Berbee (2006) were the first to provide ITS and LSU sequence data for Helicoma vaccinii (CBS 216.90), though without a morphological description. Later, CBS 216.90 was initially identified as Helicosporium vaccinii (Boonmee et al. 2014; Brahmanage et al. 2017; Chaiwan et al. 2017; Lu et al. 2017a). Following morphological comparisons to other asexual helicosporous species, Lu et al. (2018b) re-identified CBS 216.90 as Helicoma vaccinii, now C. vaccinii. Recently, Han et al. (2025) introduced three new species of Camporesiomyces: C. bhatii, C. coffeae and C. puerensis, which were isolated from dead branches of Coffea arabica and Coffea liverica in terrestrial habitats in Yunnan Province, China. Currently, six species are accepted within the genus Camporesiomyces (Han et al. 2025).

Morphologically, species of Camporesiomyces exhibit both asexual and sexual morphs, with two distinct asexual morphs (Carris 1989; Matsushima 1993; Zhao et al. 2007; Hyde et al. 2020; Han et al. 2025). The sexual morph is characterised by multi-loculate, black, subglobose to conical ascomata, bitunicate, fissitunicate, cylindrical asci and narrowly fusiform, hyaline, multi-septate ascospores (Hyde et al. 2020). The asexual morphs are characterised as follows: type 1) macronematous, mononematous, branches or unbranched, septate conidiophores, monoblastic or polyblastic, denticulate conidiogenous cells, and helicoid, solitary, guttulate, septate, hyaline conidia (Carris 1989; Matsushima 1993; Zhao et al. 2007; Hyde et al. 2020); and type 2) macronematous, mononematous, erect, solitary, unbranched septate, conidiophores, polyblastic, integrated, denticulate, terminal conidiogenous cells and acrogenous, solitary, cylindrical, obclavate or fusiform, septate, guttulate conidia (Han et al. 2025).

In this study, four asexual isolates, representing two distinct taxa, were obtained from decaying wood in terrestrial habitats in Qizhifeng Forest Park and Yushe National Forest Park, Guizhou Province. Based on morphological characteristics, illustrations and phylogenetic analyses using Maximum Likelihood and Bayesian Inference of combined ITS, LSU, tef1-α and rpb2 sequence data, two novel species are introduced, namely, Camporesiomyces qizhifengensis and C. yusheensis.

Materials and methods

Sample collection, examination and isolation

Decaying wood samples were collected in November 2024 from Liupanshui City, Guizhou Province, south-western China. Fresh samples were transported to the laboratory in plastic bags with the collection details, including localities, habitats and dates (Rathnayaka et al. 2024). The microscopic features were examined and photographed using a stereomicroscope (SMZ-168, Nikon, Japan) and an ECLIPSE Ni compound microscope (Nikon, Tokyo, Japan) with a Canon 90D digital camera. Measurements were made using Tarosoft (R) Image Frame Work software. Photo-plates were made using Adobe Photoshop CC 2019 (Adobe Systems, USA).

Single conidium isolates were done on PDA (potato dextrose agar) plates following the methods described by Chomnunti et al. (2014) and Senanayake et al. (2020) and the germinated conidia were aseptically transferred to fresh PDA plates. Morphological characteristics of fungal mycelium on PDA, including colony colour, hyphal shape and growth dimensions, were recorded. Dried fungal specimens were deposited in the Herbarium of Kunming Institute of Botany, Chinese Academy of Sciences (Herb. HKAS) in Kunming, China and Herbarium of Guizhou Academy of Agriculture Sciences (Herb. GZAAS), Guiyang, China. Pure cultures were preserved in the Guizhou Culture Collection, China (GZCC), Guiyang, China. The MycoBank numbers were obtained as described in https://www.mycobank.org/.

DNA extraction, PCR amplification and sequencing

Fresh fungal mycelium was scraped from colonies grown on PDA plates and transferred to a 1.5 ml microcentrifuge tube using a sterilised lancet for genomic DNA extraction. Genomic DNA was extracted using the Biospin Fungus Genomic DNA Extraction Kit (BioFlux, China). The following primer pairs were used to amplify specific gene regions: ITS5/ITS4 for the internal transcribed spacer (ITS; White et al. 1990), LR0R/LR5 for the large ribosomal subunit (LSU; Vilgalys and Hester 1990), EF1-983F/EF1-2218R for translation elongation factor 1-α (tef1-α; Rehner and Buckley 2005) and fRPB2-5F/fRPB2-7cR for RNA polymerase II second largest subunit (rpb2; Liu et al. 1999). DNA preparation was conducted in a 25 μl mixture, which included 1 μl of DNA, 1 μl of each forward and reverse primer and 22 μl of 1.1× T3 Super PCR Mix (Qingke Biotech, Chongqing, China). Polymerase chain reaction (PCR) was performed using the cycling conditions described by Ma et al. (2024a). The PCR products were purified and sequenced with the same primers at Beijing Tsingke Biotechnology Co., Ltd.

Phylogenetic analyses

The newly-obtained sequences were quality-checked and assembled using BioEdit v.7.0.5.3 (Hall 1999) and SeqMan v.7.0.0 (DNASTAR, Madison, WI, USA; Swindell and Plasterer 1997), respectively. The sequences used in this study were retrieved from GenBank (Table 1; https://www.ncbi.nlm.nih.gov/). Sequence matrices for each gene were aligned using MAFFT v.7.473 (https://mafft.cbrc.jp/alignment/server/; Katoh et al. (2019)). Each gene dataset was trimmed using trimAl v.1.2rev59 software (Capella-Gutiérrez et al. 2009). A concatenated sequence dataset was generated using SequenceMatrix-Windows-1.7.8 software (Vaidya et al. 2011).

Table 1.
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Taxa used in this study and their GenBank accession numbers.

Taxon Strain GenBank Accession Numbers References
ITS LSU tef1-α rpb2
Acanthohelicospora aurea GZCC 16-0060 KY321323 KY321326 KY792600 MF589911 Lu et al. (2017a)
Acanthostigma chiangmaiensis MFLUCC 10-0125T JN865209 JN865197 KF301560 N/A Boonmee et al. (2014)
Acanthostigma perpusillum UAMH 7237 AY916492 AY856892 N/A N/A Tsui et al. (2006)
Berkleasmium aquaticum MFLUCC 17-0049T KY790444 KY790432 KY792608 MF535268 Lu et al. (2017b)
Berkleasmium fusiforme MFLUCC 17-1978T MH558693 MH558820 MH550884 MH551007 Lu et al. (2018b)
Boerlagiomyces macrospora MFLUCC 12-0388 KU144927 KU764712 KU872750 N/A Doilom et al. (2017)
Botryosphaeria agaves MFLUCC 10-0051 JX646790 JX646807 N/A N/A Liu et al. (2012)
Botryosphaeria dothidea CBS 115476 KF766151 DQ678051 DQ767637 DQ677944 Slippers et al. (2013)
Camporesiomyces bhatii GMBCC 1120T PQ763360 PQ842543 PV388894 PV388888 Han et al. (2025)
Camporesiomyces bhatii GMBCC 1125 PQ763361 PQ842544 PV388895 PV388889 Han et al. (2025)
Camporesiomyces coffeae GMBCC 1130T PQ763358 PQ842545 PV388896 PV388890 Han et al. (2025)
Camporesiomyces coffeae GMBCC 1131 PQ763359 PQ842546 PV388897 PV388891 Han et al. (2025)
Camporesiomyces mali KUMCC 19-0216T NR_169709 NG_075312 MN794018 N/A Hyde et al. (2020)
Camporesiomyces patagonicus BBB MVB 573 JN127358 JN127359 N/A N/A Hyde et al. (2020)
Camporesiomyces puerensis GMBCC 1113T PQ763356 PQ842541 PV388892 PV388886 Han et al. (2025)
Camporesiomyces puerensis GMBCC 1114 PQ763357 PQ842542 PV388893 PV388887 Han et al. (2025)
Camporesiomyces qizhifengensis GZCC 25-0638T PX111185 PX111192 PX102609 N/A In this study
Camporesiomyces qizhifengensis GZCC 25-0639 PX111186 PX111193 PX102610 N/A In this study
Camporesiomyces vaccinii CBS 216.90 MH862204 MH873889 N/A N/A Hyde et al. (2020)
Camporesiomyces yusheensis GZCC 25-0636T PX111183 PX111190 PX102607 PX102601 In this study
Camporesiomyces yusheensis GZCC 25-0637 PX111184 PX111191 PX102608 PX102602 In this study
Chlamydotubeufia cylindrica MFLUCC 16-1130T MH558702 MH558830 MH550893 MH551018 Lu et al. (2018b)
Chlamydotubeufia huaikangplaensis MFLUCC 10-0926T JN865210 JN865198 N/A N/A Boonmee et al. (2011)
Dematiohelicomyces helicosporus MFLUCC 16-0213T KX454169 KX454170 KY117035 MF535258 Hyde et al. (2016)
Dematiohelicosporum guttulatum MFLUCC 17-2011T MH558705 MH558833 MH550896 MH551021 Lu et al. (2018b)
Dematiotubeufia chiangraiensis MFLUCC 10-0115T JN865200 JN865188 KF301551 N/A Boonmee et al. (2011)
Helicangiospora lignicola MFLUCC 11-0378T KF301523 KF301531 KF301552 N/A Boonmee et al. (2014)
Helicoarctatus aquaticus MFLUCC 17-1996T MH558707 MH558835 MH550898 MH551024 Lu et al. (2018b)
Helicohyalinum aquaticum MFLUCC 16-1131T KY873625 KY873620 KY873284 MF535257 Lu et al. (2018b)
Helicohyalinum infundibulum MFLUCC 16-1133T MH558712 MH558840 MH550903 MH551029 Lu et al. (2018b)
Helicoma guttulatum MFLUCC 16-0022T KX454171 KX454172 MF535254 MH551032 Hyde et al. (2016)
Helicoma hongkongense MFLUCC 17-2005 MH558716 MH558843 MH550907 MH551033 Lu et al. (2018b)
Helicosporium acropleurogenum CGMCC 3.25563T PP626574 PP639430 PP596333 PP596460 Ma et al. (2024b)
Helicosporium aquaticum MFLUCC 17-2008T MH558733 MH558859 MH550924 MH551049 Lu et al. (2018b)
Helicosporium brunneisporum CGMCC 3.25542T PP626577 PP639433 PP596336 PP596463 Ma et al. (2024b)
Helicosporium changjiangense GZCC 22-2113T PP626578 PP639434 PP596337 PP596464 Ma et al. (2024b)
Helicosporium flavisporum MFLUCC 17-2020T MH558734 MH558860 MH550925 MH551050 Lu et al. (2018b)
Helicosporium ramosiphorum CGMCC 3.25541T PP626576 PP639432 PP596335 PP596462 Ma et al. (2024b)
Helicosporium rubrum MFLUCC 24-0090T PQ098477 PQ098514 PQ490681 PQ490675 Peng et al. (2025)
Helicosporium setiferum MFLUCC 17-1994T MH558735 MH558861 MH550926 MH551051 Tsui et al. (2006)
Helicosporium sexuale MFLUCC 16-1244T MZ538503 MZ538537 MZ567082 MZ567111 Boonmee et al. (2011)
Helicotubeufia hydei MFLUCC 17-1980T MH290021 MH290026 MH290031 MH290036 Liu et al. (2018)
Helicotubeufia jonesii MFLUCC 17-0043T MH290020 MH290025 MH290030 MH290035 Liu et al. (2018)
Muripulchra aquatica MFLUCC 15-0249T KY320532 KY320549 N/A N/A Luo et al. (2017)
Neoacanthostigma fusiforme MFLUCC 11-0510T KF301529 KF301537 N/A N/A Boonmee et al. (2014)
Neochlamydotubeufia fusiformis MFLUCC 16-0016T MH558740 MH558865 MH550931 MH551059 Lu et al. (2018b)
Neohelicomyces acropleurogenus CGMCC 3.25549T PP626594 PP639450 PP596351 PP596478 Ma et al. (2024b)
Neohelicomyces aquaticus MFLUCC 16-0993T KY320528 KY320545 KY320561 MH551066 Luo et al. (2017)
Neohelicosporium acrogenisporum MFLUCC 17-2019T MH558746 MH558871 MH550937 MH551069 Lu et al. (2018b)
Neohelicosporium aquaticum MFLUCC 17-1519T MF467916 MF467929 MF535242 MF535272 Lu et al. (2018a)
Neomanoharachariella xizangensis KUNCC 23-15799T OR803724 OR803722 OR813978 OR813975 Yang et al. (2024)
Parahelicomyces quercus MFUCC 17-0895T MK347720 MK347934 MK360077 MK434906 Hsieh et al. (2021)
Parahelicomyces talbotii MFLUCC 17-2021T MH558765 MH558890 MH550957 MH551091 Hsieh et al. (2021)
Tubeufia guttulata GZCC 23-0404T OR030841 OR030834 OR046678 OR046684 Ma et al. (2023c)
Tubeufia hainanensis GZCC 22-2015T OR030842 OR030835 OR046679 OR046685 Ma et al. (2023c)
Zaanenomyces moderatricis-academiae CPC 41273T OK664723 OK663762 N/A OK651167 Crous et al. (2023)
Zaanenomyces versatilis CPC 41224T OK664730 OK663769 N/A N/A Crous et al. (2023)

Note: “T” indicates ex-type strains. Newly-generated sequences are in bold. “N/A” indicates the unavailable data in GenBank.

Maximum Likelihood (ML) analysis was performed using IQ-TREE web server (http://iqtree.cibiv.univie.ac.at/) with the best-fit substitution model automatically selected based on the Bayesian Information Criterion (BIC) (Nguyen et al. 2015). Bayesian Inference (BI) analysis was conducted by using MrBayes on XSEDE (3.2.7a) via CIPRES (Stamatakis 2014). The aligned FASTA file was converted to a Nexus format file using AliView (Daniel et al. 2010). The optimal substitution model for each dataset was determined using MrModelTest v.2.3 (Nylander et al. 2008). The posterior probabilities (BYPP) were determined, based on Bayesian Markov Chain Monte Carlo (BMCMC) sampling (Huelsenbeck and Ronquist 2001). Four simultaneous Markov chains were run for 10,000,000 generations and trees were sampled every 1,000th generation. The burn-in phase was set at 25% and the remaining trees were used for calculating posterior probabilities (BYPP).

Phylogenetic trees were visualised using FigTree v.1.4.4 and subsequently edited using Adobe Illustrator CC 2019 (v.23.1.0; Adobe Systems, USA).

Phylogenetic results

The phylogenetic positions of the four novel strains were determined through multi-locus phylogenetic analysis. The concatenated sequence matrix comprised 3,401 characters (ITS: 1–583, LSU: 584–1,444, tef1-α: 1,445–2,356, and rpb2: 2,357–3,401) across 57 taxa. Fig. 1 shows the best-scoring Maximum Likelihood (ML) tree with a final log-likelihood value of -33524.456.

Figure 1. 

Phylogenetic tree generated from the ML analysis, based on the combined ITS, LSU, tef1-α and rpb2 sequence data. Bootstrap support values of ML equal to or greater than 75% and Bayesian posterior probabilities (PP) equal to or greater than 0.95 are given near the nodes as ML/BYPP, respectively. The Maximum Likelihood (ML) and Bayesian Inference (BI) analyses yielded similar tree topologies. Hyphen (“-”) indicates a value lower than 75% for ML and a posterior probability lower than 0.95 for BI. Botryosphaeria agaves (MFLUCC 10-0051) and B. dothidea (CBS 115476) were selected as outgroups (Lu et al. 2018b). Ex-type strains are denoted with “T” and newly-obtained isolates are in bold black fonts.

Based on concatenated phylogenetic analysis of ITS, LSU, tef1-α and rpb2 loci (Fig. 1), our isolates represent two distinct species of Camporesiomyces, forming well-supported clades separate from known taxa. Isolates GZCC 25-0638 and GZCC 25-0639 formed a well-supported clade (ML = 94%, BYPP = 1.00) sister to C. patagonicus (BBB MVB 573). Additionally, isolates GZCC 25-0636 and GZCC 25-0637 formed a distinct lineage with C. bhatii (GMBCC 1120 and GMBCC 1125) with 90% ML and 1.00 BYPP support.

Taxonomy

Camporesiomyces qizhifengensis Song Bai, Rong Wu & Jian Ma, sp. nov.

MycoBank No: 904188
Fig. 2.

Etymology.

‘‘qizhifengensis” refers to the ‘‘Qizhifeng Forest Park” where the holotype was collected.

Holotype.

HKAS 128896.

Description.

Saprobic on decaying wood in a terrestrial habitat. Sexual morph Not seen. Asexual morph hyphomycetous. Colonies on natural substratum superficial, effuse, scattered or aggregated, hairy, yellow. Mycelium partly superficial, partly immersed, composed of branched, septate, guttulate, smooth-walled, hyaline to brown hyphae. Conidiophores 65–122 × 4–6 μm (x̄ = 88 × 5.2 μm, n = 25), macronematous, mononematous, erect, solitary, smooth or occasionally verruculose, cylindrical, dark brown, paler towards apex, slightly flexuous, unbranched, 4–11-septate, sometimes slightly constricted at septa. Conidiogenous cells 10–18 × 2.8–6 μm (x̄ = 14.5 × 3.7 μm, n = 25), polyblastic, integrated, terminal, determinate, cylindrical, slightly tapering, conspicuously denticulate at conidial secession, subhyaline to pale brown. Conidia 11–16.2 × 3.9–5.2 μm (x̄ = 13.3 × 4.6 μm, n = 30), acrogenous, solitary, cylindrical, obclavate or fusiform, 0–4-septate, guttulate, subhyaline to yellowish-brown, slightly constricted at septa.

Figure 2. 

Camporesiomyces qizhifengensis (HKAS 128896, holotype). a–c. Colonies on the host surface; d–h. Conidiophores and conidiogenous cells; i–p. Conidia; q. Germinated conidium; r, s. Colonies on PDA from below and above after 39 days of incubation at room temperature. Scale bars: 20 μm (d–h); 5 μm (i–q).

Culture characteristics.

Conidia germinating on PDA within 18 hours, producing germ tubes from apices. Colony on PDA reaching 3 cm diam. after 39 days at room temperature (approximately 25 °C), circular or irregular, umbonate, with undulate margin, brown to dark brown, reverse pale brown to brown.

Material examined.

China • Guizhou Province, Liupanshui City, Dashan Town, Qizhifeng Forest Park, on decaying wood in a terrestrial habitat, 27 November 2024, Xia Tang, LQ05 (HKAS 128896, holotype; GZAAS 25–0669, isotype), ex-type living culture GZCC 25–0638; • Ibid., LQ09 (GZAAS 25–0666, paratype), living culture GZCC 25–0639.

Notes.

Camporesiomyces qizhifengensis (HKAS 128896) exhibits morphological similarities to C. coffeae, particularly in conidiophore and conidial morphology (Han et al. 2025). However, this species differs from C. coffeae in having longer conidiophores (up to 122 μm vs. 43–97 μm) and shorter conidia (11–16.2 μm vs. 20–50 μm) (Han et al. 2025). Our multi-gene phylogenetic analysis strongly supports that our isolates (GZCC 25–0638 and GZCC 25–0639) form a sister clade to C. patagonicus, with 94% ML and 1.00 BYPP support (Fig. 1). Additionally, base pair comparison between C. qizhifengensis and C. patagonicus reveals 14/322 bp differences in ITS (4.3%, including six gaps) and 6/613 bp differences in LSU (0.9%, without gaps), further supporting their distinction as separate species. The sexual state of C. patagonicus is the only known form of this species (Sánchez et al. 2012; Hyde et al. 2020; Ma et al. 2024b). Therefore, based on both the multi-gene phylogenetic analysis and morphological differences, we introduce Camporesiomyces qizhifengensis as a new species.

Camporesiomyces yusheensis Song Bai, Rong Wu & Jian Ma, sp. nov.

MycoBank No: 904189
Fig. 3.

Etymology.

‘‘yusheensis” refers to the ‘‘Yushe National Forest Park” where the holotype was collected.

Holotype.

HKAS 128898.

Description.

Saprobic on decaying wood in a terrestrial habitat. Sexual morph Not seen. Asexual morph hyphomycetous. Colonies on natural substratum superficial, effuse, scattered or aggregated, hairy, yellow at apex. Mycelium partly superficial, partly immersed, composed of branched, septate, guttulate, smooth-walled, hyaline to brown hyphae. Conidiophores 51–97 × 3.7–5.8 μm (x̄ = 69 × 4.6 μm, n = 30), macronematous, mononematous, erect, solitary, smooth or occasionally verruculose, cylindrical, dark brown, paler towards apex, slightly flexuous, unbranched, 4–12-septate, sometimes slightly constricted at septa. Conidiogenous cells 9.6–17.8 × 3.3–4.1 μm (x̄ = 13.4 × 3.6 μm, n = 25), polyblastic, integrated, terminal, determinate, cylindrical, slightly tapering, conspicuously denticulate at conidial secession, subhyaline to pale brown. Conidia 15.4–23 × 4.5–6.8 μm (x̄ = 19.3 × 5.3 μm, n = 35), acrogenous, solitary, cylindrical, obclavate or fusiform, 0–4-septate, mostly 3–4-septate, slightly flexuous, guttulate, brown to yellowish-brown.

Figure 3. 

Camporesiomyces yusheensis (HKAS 128898, holotype). a, b. Colonies on the host surface; c–f. Conidiophores and conidiogenous cells; g–n. Conidia; o. Germinated conidium; p, q. Colonies on PDA from above and below after 41 days of incubation at room temperature. Scale bars: 20 μm (c–f); 10 μm (g–j); 5 μm (k–o).

Culture characteristics.

Conidia germinating on PDA within 15 hours, producing germ tubes from apices. Colony on PDA reaching 3.2 cm diam. after 41 days at room temperature (approximately 25 °C), circular or irregular, umbonate, with undulate margin, pale brown to black, reverse black.

Material examined.

China • Guizhou Province, Liupanshui City, Shuicheng County, Yushe National Forest Park, on decaying wood in a terrestrial habitat, 27 November 2024, Xia Tang, LS53 (HKAS 128898 holotype; GZAAS 25–0667, isotype), ex-type living culture GZCC 25–0636; Ibid., LS63 (GZAAS 25–0668, paratype), living culture GZCC 25–0637.

Notes.

In the phylogenetic analyses (Fig. 1), Camporesiomyces yusheensis formed a sister clade to C. bhatii with 90% ML and 1.00 BYPP support. However, a comparison of nucleotides in the ITS, LSU, tef1-α and rpb2 sequence between C. yusheensis and C. bhatii, revealed nucleotide differences of 25/494 bp (5.1%, including five gaps), 4/855 bp (0.5%, without gaps), 61/910 bp (6.7%, without gaps) and 87/958 bp (9.1%, without gaps), respectively. Moreover, C. yusheensis differs from C. bhatii by its shorter conidiogenous cells (9.6–17.8 μm vs. up to 21.7 μm) and shorter conidia (15.4–23 μm vs. up to 30 μm) (Han et al. 2025). Therefore, based on ITS, LSU, tef1-α and rpb2 sequence data and morphological characteristics, we introduce Camporesiomyces yusheensis as a new species.

Discussion

Prior to this study, six species were recognised within Camporesiomyces and, with the addition of our two newly-described species, the genus now comprises eight species (Carris 1989; Matsushima 1993; Tsui and Berbee 2006; Zhao et al. 2007; Sánchez et al. 2012; Lu et al. 2018b; Hyde et al. 2020; Han et al. 2025). All Camporesiomyces species are found in terrestrial habitats, with primary distributions in Guizhou, Jilin and Yunnan Provinces of China and additional records from Argentina, China, Peru and the USA. They occur as saprobes on Coffea arabica, C. liberica, Malus halliana, Nothofagus alpina, palma and Vaccinium elliotii and decaying wood in terrestrial habitats.

Based on DNA molecular evidence, some helicosporous genera within Tubeufiaceae exhibit a wide range of conidial morphologies in their asexual reproductive forms (Lu et al. 2018b; Ma et al. 2024b; Han et al. 2025). For example, Tubeufia is characterised by muriform, dorsiventrally curved, coiled, ovate or ellipsoid, globose to subglobose and ovoid to irregular conidia, while Camporesiomyces is characterised by cylindrical, obclavate or fusiform or helicoid conidia (Carris 1989; Matsushima 1993; Zhao et al. 2007; Lu et al. 2018b; Ma et al. 2024b; Hyde et al. 2020; Han et al. 2025). This morphological diversification may be explained by molecular stasis, whereby genetic sequences remain relatively conserved while species undergo morphological adaptations in response to environmental pressures.

Morphologically, Camporesiomyces vaccinii resembles species of Helicoma, particularly in conidiophore and conidial features (Lu et al. 2018b; Ma et al. 2024b). However, C. vaccinii can be distinguished from Helicoma species by its long basal cell, which tapers towards the narrow base of the conidia (Carris 1989; Matsushima 1993; Zhao et al. 2007; Hyde et al. 2020; Han et al. 2025). Additionally, C. vaccinii differs from other asexual species of Camporesiomyces by possessing helicoid conidia (Carris 1989; Matsushima 1993; Zhao et al. 2007; Hyde et al. 2020; Han et al. 2025).

Acknowledgements

We would like to thank Shaun Pennycook (Manaaki Whenua Landcare Research, New Zealand) for his valuable suggestions on the fungal nomenclature.

Additional information

Conflict of interest

The authors have declared that no competing interests exist.

Ethical statement

No ethical statement was reported.

Use of AI

No use of AI was reported.

Funding

This work was supported by Guizhou Industry Polytechnic College Faculty-level Research Project (Grant No. 2024ZK18), the Science and Technology Planning Project of Guizhou Province (Grant No. Qian Ke He Ji Chu ZK [2022] Zhong Dian 025), High-Level Talent Initial Funding of Guizhou Industry Polytechnic College (Grant No. 2023-RC-01).

Author contributions

Morphological data, photo-plates and phylogenetic analyzes were completed by Song Bai and Jian Ma. The original draft was written by Song Bai and Jian Ma, and Fang Wang, Su-Ran Wan , Xiao-Kang Lv, Li-Jun Chen , Rong Wu revised the paper.

Author ORCIDs

Song Bai https://orcid.org/0000-0002-1972-2834

Fang Wang https://orcid.org/0000-0002-3341-6788

Su-Ran Wan https://orcid.org/0009-0000-1164-6921

Xiao-Kang Lv https://orcid.org/0009-0006-7254-8747

Li-Jun Chen https://orcid.org/0009-0004-8562-875X

Rong Wu https://orcid.org/0000-0002-4946-8806

Jian Ma https://orcid.org/0009-0008-1291-640X

Data availability

All of the data that support the findings of this study are available in the main text.

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