Corresponding author: Yong-Jun Wu (
Academic editor: Huzefa Raja
Two new species,
Tong S-Q, Peng L, Wu Y-J (2023)
In this study, seven strains of
The purified isolates were transferred to potato dextrose agar (
Total DNA was extracted from each of the new isolates using the BioTeke Fungus Genomic DNA Extraction kit (DP2032, BioTeke, Beijing, China) according to the manufacturer’s instructions. According to
Strains included in the present study.
Species | Strains |
|
|
|
|
|
---|---|---|---|---|---|---|
|
CBS 407.66 T |
|
|
|||
|
CBS 831.97 |
|
||||
|
MFLU 18-1225 T |
|
|
|
||
|
CBS 146824 T |
|
|
|||
|
CBS 750.69 T |
|
||||
|
CBS 993.69 |
|
|
|||
|
CBS 101148 T |
|
|
|||
|
CBS 866.73 T |
|
|
|||
|
CBS 756.69 T |
|
|
|||
|
CBS 101149 T |
|
|
|||
|
CBS 111656 |
|
|
|||
|
CBS 758.69 |
|
|
|||
|
CBS 207.65 |
|
||||
|
CBS 215.69 |
|
||||
|
MFLUCC 14-0397 T |
|
|
|||
|
CBS 144.62 T |
|
|
|||
|
CBS 401.65 |
|
|
|||
|
CBS 384.96 T |
|
|
|||
|
CGMCC 3.20954 T |
|
|
|
|
|
|
CBS 139050 T |
|
|
|||
|
CBS 587.73 T |
|
||||
|
UAMH 9995 T |
|
|
|||
|
CBS 147.81 T |
|
|
|||
|
CBS 596.70 T |
|
|
|||
|
CBS 316.72 |
|
|
|||
|
CBS 112868 T |
|
||||
|
CBS 113889 |
|
||||
|
CBS 109069 |
|
|
|||
|
CBS 991.69 |
|
|
|||
|
CBS 840.68 T |
|
|
|||
|
CGMCC 3.20955 T |
|
|
|
|
|
|
GZUIFR 22.037 |
|
|
|
|
|
|
GZUIFR 22.038 |
|
|
|
|
|
|
CBS 390.73 |
|
|
|||
|
CBS 768.69 T |
|
|
|||
|
CBS 212.69 T |
|
||||
|
ACCC 39305 T |
|
|
|||
|
CBS 139051 T |
|
|
|||
|
FMR 10363 |
|
|
|||
|
CBS 381.70A |
|
|
|||
|
CBS 310.59 T |
|
||||
|
CBS 101694 |
|
||||
|
CBS 157.70 T |
|
|
|||
|
CBS 181.27 T |
|
|
|||
|
CBS 189.70 |
|
||||
|
CBS 560.73 T |
|
||||
|
CBS 782.69 T |
|
||||
|
CBS 784.69 |
|
||||
|
CBS 124.42 T |
|
|
|
||
|
A101 |
|
|
|
|
|
|
A130 |
|
|
|
|
|
E102 |
|
|
|
|
|
|
|
CBS 136.33 T |
|
|
|
||
|
CBS 138820 T |
|
||||
|
CBS 127532 T |
|
|
|||
|
CBS 130360 T |
|
|
|||
|
CBS 130361 |
|
|
|||
|
CBS 989.69 T |
|
||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
M139 |
|
|
|||
|
M190 |
|
|
|||
|
M140 |
|
||||
|
CBS 318.91 T |
|
|
|||
|
CBS 149.62 T |
|
||||
|
CBS 530.68 T |
|
||||
|
CBS 127350 T |
|
|
|
|
|
|
CBS 120.40 T |
|
|
|
||
|
CBS 134.56 T |
|
||||
|
ATCC 22087 T |
|
|
|||
|
CBS 430.66 T |
|
|
|
||
|
CBS 230.31 |
|
||||
|
CBS 737.84 T |
|
|
|||
|
ATCC 22102 T |
|
||||
|
CBS 110348 T |
|
|
|
||
|
CBS 150.62 T |
|
||||
|
CBS 142.62 T |
|
|
|||
|
CBS 114491 T |
|
|
|
||
|
CBS 356.86 T |
|
|
|
||
|
CBS 137990 T |
|
|
|||
|
CBS 100582 |
|
|
|
||
|
CBS 425.67 T |
|
|
|
||
|
CBS 749.69 T |
|
|
|
||
|
CBS 346.70 T |
|
|
|
||
|
CBS 243.59 T |
|
|
|||
|
AR 2812 |
|
|
|
||
|
CBS 129.64 T |
|
|
|||
|
CBS 123.78T |
|
|
|||
|
DAOM 208997 |
|
|
|||
|
ATCC 36477 |
|
|
Notes: “T” stands for Ex-type strains. New isolates are in bold and blue.
In this study, we utilized sequence data mainly from recent publications (
The best-fit substitution model was selected using the corrected Akaike information criterion, in ModelFinder (
Ninety-five isolates (including the seven with new sequence data) were included in our dataset (Table
Phylogram generated from maximum likelihood analysis based on
Referring to the type strain isolated from the rhizosphere soil of
Guiyang City, Guizhou Province, China
Culture characteristics (14 days at 25 °C) – Colonies on
Guiyang City, Guizhou Province, China
Guiyang City, Guizhou Province, China.
In a phylogenetic tree based on
Morphology of
Referring to the country where this fungus was first isolated.
Guiyang City, Guizhou Province, China
Culture characteristics (14 days at 25 °C) – Colonies on
Guiyang City, Guizhou Province, China
Guiyang City, Guizhou Province, China.
Phylogenetic and morphological data (Figs
Morphology of
Traditionally, a polyphasic approach based on morphology, physiology, biochemistry, or reactions to chemical tests, has been used to differentiate species (
Members of the genus
In summary, seven isolates of
This study was financially supported by Guizhou Provincial Science and Technology Projects (Qian Ke He Zhi Cheng-ZK[2022] General 172, and ZK[2021] General 262), and Guizhou Provincial Institutions of higher learning Engineering Center Projects (Qian Jiao He KY[2021]006). We appreciate Charlesworth for English-language editing of the whole manuscript.