Corresponding authors: Qi-Rui Li (
Academic editor: Chitrabhanu Bhunjun
Several micro fungi were gathered from bamboo and palm in Guizhou Province, China. In morphology, these taxa resemble
Hu HM, Liu LL, Zhang X, Lin Y, Shen XC, Long SH, Kang JC, Wijayawardene NN, Li QR, Long QD (2022) New species and records of
Ascomycetous taxa on bamboo and palm are commonly observed with immersed ascomata (
The family
In this study, several specimens of bamboo and palm were collected from Guizhou Province. Based on their morphology and phylogeny, two new species and two new records from China are herein reported. Full descriptions, photo plates of macro-and micro-morphological characteristics and a phylogenetic tree to show the phylogenetic placement of the new records and the new species are provided.
From 2021 to 2022, fresh materials were collected from bamboo and palms in forests and nature reserves of Guizhou Province, China, and returned to the lab in paper or plastic bags. Samples were treated and examined with the method described by
Isolations were made by single spore isolation (
The OMEGA E.Z.N.A. Fungal Genomic DNA Extraction Kit (D3390, Guangzhou Feiyang Bioengineering Co., Ltd, China) was used to extract genomic DNA from fresh fungal mycelium, according to the manufacturer’s instructions. The extracted DNA was stored at –20 °C.
ITS5/ITS4 (
Taxa of
Species | Strain number | GenBank Accession number | References | ||||
---|---|---|---|---|---|---|---|
ITS | LSU | SSU | |||||
|
RS10 |
|
NA | NA | NA | NA |
|
|
RS9 |
|
NA | NA | NA | NA |
|
|
NA |
|
|
|
NA |
|
|
|
NA |
|
|
NA | NA |
|
|
|
NA |
|
|
|
|
|
|
|
|
|
NA | NA | NA |
|
|
|
NA |
|
NA | NA | NA |
|
|
|
NA |
|
|
NA | NA | Unpublished | |
|
NA |
|
|
NA | NA |
|
|
|
|
|
NA | NA | NA |
|
|
|
NA |
|
NA | NA | NA |
|
|
|
NA |
|
|
NA | NA |
|
|
|
|
|
NA |
|
|
|
|
|
NA |
|
|
NA | NA | Unpublished | |
|
NA |
|
|
|
|
|
|
|
|
|
NA | NA | NA |
|
|
|
CBS138003 |
|
|
NA | NA | NA |
|
|
CBS137976 |
|
|
NA | NA | NA |
|
|
|
|
|
NA | NA |
|
|
|
NA |
|
|
NA | NA |
|
|
|
UAMH11631 (ET) |
|
|
NA | NA | NA | Unpublished |
|
HKUCC6349 (ET) | NA |
|
|
NA | NA | Unpublished |
|
LC0555 |
|
|
|
NA | NA |
|
|
Co108 |
|
|
NA | NA | NA |
|
|
Co117 |
|
|
NA | NA | NA |
|
|
Co126 (ET) |
|
|
NA | NA | NA |
|
|
PE 0063-1a | NA |
|
NA | NA | NA |
|
|
|
|
NA | NA | NA | Unpublished | |
|
|
|
NA | NA | NA | Unpublished | |
|
CM AT-018 | NA |
|
NA | NA | NA | Unpublished |
|
WM10.89 | NA |
|
NA | NA | NA | Unpublished |
|
|
|
NA | NA | NA |
|
|
|
UME 31411 | NA |
|
|
NA | NA |
|
|
NA |
|
|
|
|
|
|
|
NA |
|
|
NA | NA |
|
|
|
|
|
|
NA | NA |
|
|
|
|
NA | NA | NA | NA | Unpublished | |
|
NA | NA |
|
|
NA |
|
|
|
|
NA | NA | NA | NA |
|
|
|
NA |
|
|
|
|
|
|
|
|
|
|
NA | NA |
|
|
|
UME 31430 | NA |
|
|
NA | NA | Unpublished |
|
HHUF 29920 |
|
|
|
NA | NA |
|
|
MFLU15-0393 |
|
|
|
NA | NA | Li et al. (2015) |
|
NA |
|
|
NA | NA |
|
|
|
NA |
|
|
|
|
|
|
|
LT2 |
|
NA | NA | NA | NA | Voglmayr et al. (2017) |
|
NA |
|
|
|
|
|
|
|
NA |
|
|
|
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
NA | NA |
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
|
|
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
NA | NA |
|
|
|
|
NA |
|
|
NA | NA |
|
|
|
NA |
|
NA | NA |
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
NA | NA |
|
|
|
|
NA |
|
NA | NA |
|
|
|
|
NA |
|
|
|
|
|
|
|
NA |
|
|
NA |
|
|
|
|
NA |
|
|
|
|
|
|
|
NA |
|
|
|
|
|
|
|
MBU (ET) |
|
NA | NA | NA | NA |
|
|
|
|
NA | NA | NA |
|
|
|
|
|
NA | NA | NA | Hernandez et al. (2016) | |
|
FMR6682 | NA | NA |
|
NA | NA |
|
|
NA |
|
|
NA | NA | Mapook et al. (2016) | |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
NTUCC 17-007 | NA |
|
|
NA | NA | Ariyaw et al. (2018) |
|
NA |
|
|
NA | NA |
|
|
|
|
|
NA |
|
|
|
|
|
|
|
NA | NA | NA |
|
|
|
|
|
NA | NA | NA |
|
|
|
NA |
|
|
|
|
|
|
|
NA |
|
|
NA | NA |
|
|
|
FIH 151 |
|
|
NA | NA | NA |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
FIH 018 |
|
|
NA | NA | NA |
|
|
|
|
|
NA | NA |
|
|
|
|
|
|
NA | NA |
|
|
|
|
|
NA | NA | NA | Unpublished | |
|
NA |
|
|
NA | NA |
|
|
|
NA |
|
|
NA |
|
Samarak and Hyde (2019) | |
|
|
|
NA |
|
|
|
|
|
|
NA | NA | NA | NA |
|
|
|
|
|
NA | NA | NA |
|
|
|
|
|
NA |
|
|
|
|
|
|
NA |
|
|
|
|
|
|
|
|
NA | NA |
|
Unpublished | |
|
SAJ-0021 (ET) |
|
|
|
NA | NA | Unpublished |
|
|
|
NA | NA | NA |
|
|
|
It 1200 |
|
|
NA | NA | NA |
|
|
PC1 (ET) |
|
NA | NA | NA | NA |
|
|
|
|
NA |
|
NA |
|
|
|
|
|
NA | NA | NA |
|
|
|
|
|
NA | – | – | Unpublished | |
|
|
|
NA | NA | NA | Unpublished | |
|
|
|
NA | NA | NA |
|
|
|
|
|
NA |
|
|
|
|
|
|
|
NA | NA | NA |
|
|
|
NA |
|
|
|
|
|
|
|
|
|
NA |
|
|
|
|
|
|
|
NA |
|
|
|
|
|
|
NA | NA | NA | NA |
|
|
|
|
|
NA |
|
|
Unpublished | |
|
NA |
|
NA | NA | NA | Unpublished | |
|
MUT 5329 (HT) |
|
|
NA |
|
|
|
|
MUT5369 (HT) |
|
|
NA |
|
|
|
|
|
|
NA | NA | NA |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
NA | NA |
|
|
|
|
|
|
NA |
|
|
|
|
|
MUT 5503 (HT) | NA |
|
NA |
|
|
|
|
GMBC0009 (HT) |
|
|
NA |
|
NA | Unpublished |
|
NA |
|
NA |
|
|
|
|
|
RS12 |
|
NA | NA | NA | NA |
|
|
RS13 |
|
NA | NA | NA | NA |
|
|
|
|
NA |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
NA | NA | NA |
|
|
|
NA |
|
NA |
|
|
|
|
|
|
|
NA | NA |
|
|
|
|
|
NA | NA | NA | NA |
|
|
|
|
|
NA | NA |
|
Unpublished | |
|
|
|
NA | NA | NA |
|
|
|
|
|
NA | NA | NA |
|
|
|
|
|
NA | NA | NA | Unpublished | |
|
NA |
|
NA |
|
|
|
|
|
|
|
NA | NA | NA |
|
|
|
NA |
|
|
NA | NA |
|
|
|
|
NA | NA | NA | NA | Crous et al. (2007) | |
|
|
|
NA | NA | NA |
|
|
|
|
NA | NA |
|
|
|
|
|
NFCCl 4215 |
|
|
NA |
|
|
|
|
NFCCl 4213 |
|
|
NA |
|
|
|
|
|
|
NA |
|
|
|
|
|
NA |
|
|
|
|
|
|
|
|
|
NA | NA | NA | Senanayake et al. (2014) | |
|
BRIP 56959 (ET) |
|
|
NA | NA | NA |
|
|
|
NA | NA | NA | NA |
|
|
|
|
NA | NA | NA | NA |
|
|
|
NA |
|
|
NA | NA |
|
Notes: Type specimens are marked with HT (holotype), ET (epitype); NA: No sequence is available in GenBank; newly generated sequences are indicated in bold.
All sequences used for phylogenetic analysis were downloaded from the GenBank, based on published literature and the highest hit rate of ITS in the GenBank database. Sequence data for the construction of the phylogenetic trees are listed in Table
Three phylogenetic trees for each genus and their related genera were provided.
The dataset for Fig.
RAxML tree of
The dataset for Fig.
RAxML tree of
The alignment for Fig.
RAxML tree of
The four species in this study were
see
China, Guizhou Province, the campus of Guizhou Medical University (
China, Guizhou Province, the campus of Guizhou Medical University (
There are three
GMB0316.
In reference to the host,
Ascospores germinated on
China, Guizhou Province, Guiyang Huaxi National Urban Wetland Park (
China, Guizhou Province, Guiyang Huaxi National Urban Wetland Park (
Morphologically,
see
China, Guizhou Province, Guiyang Huaxi National Urban Wetland Park (
China, Guizhou Province, Guiyang Huaxi National Urban Wetland Park (
Phylogenetic analyses of the alignment combining ITS, LSU,
GMB0315.
In reference to the host,
Ascospores germinated on
China Guizhou Province, Long gong scenic spot (
China, Guizhou Province, Long gong scenic spot (
In this study, two new species and two new records associated with bamboo and palm were introduced based on phylogenetic relationships of combined ITS, LSU, SSU,
There are a large number of fungi associated with bamboo and palm in China (
This research was supported by the National Natural Science Foundation of China (31960005 and 32000009); the Fund of the Science and Technology Foundation of Guizhou Province ([2020]1Y059); Guizhou Province Ordinary Colleges and Universities Youth Science and Technology Talent Growth Project [2021]154.