Corresponding authors: Yong Wang (
Academic editor: Thorsten Lumbsch
Wang S-Y, Wang Y, Li Y (2022)
The fungal genus
In a recent research program, we have carried out a survey of micro-fungi associated with
Fallen leaves of
Pure cultures were grown on
Fresh mycelia were scraped from the
Sequences used in this study (Table
Taxa used for molecular phylogenetic analyses and their GenBank accession numbers. Newly generated sequences are in bold. (T) = ex-holotype strain, (ET) = ex-epitype strain, (NT) = ex-neotype strain.
Species | Strain number | Host | Country | GenBank Accession number | ||
---|---|---|---|---|---|---|
|
|
|
||||
|
CBS 140692T | Man, bronchoalveolar lavage fluid | USA |
|
|
|
|
CPC 11526 | Acacia mangium | Thailand |
|
|
|
|
CBS 140685T | Man, bronchoalveolar lavage fluid | USA |
|
|
|
|
CBS 117483 | – | USA |
|
|
|
|
CPC 22272 | Indoor air sample, ship | USA |
|
|
|
|
CBS 101367 | Soil | Brazil |
|
|
|
|
CBS 112388 | Air, indoor environment | Germany |
|
|
|
|
CBS 113738 |
|
USA |
|
|
|
|
CBS 386.64T |
|
Taiwan |
|
|
|
|
CBS 119542 |
|
Japan |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
– |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
MFLUCC 18-1559T |
|
China |
|
|
– |
|
MFLUCC 18-1557 |
|
China |
|
|
– |
|
CBS 125991T | Soil, near the terracotta army | China |
|
|
|
|
CBS 126351 | Indoor air | Venezuela |
|
|
|
|
|
|
|
|
|
|
|
CBS 125996T | South Africa |
|
|
|
|
|
CPC 12216 |
|
Germany |
|
|
|
|
CPC 14247 | USA |
|
|
|
|
|
CPC 13870 |
|
South Africa |
|
|
|
|
DTO 323-E4 | Indoor air | China |
|
|
|
|
CPC 22297 | Indoor air sample | USA |
|
|
|
|
CBS 125994T |
|
South Korea |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
– |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
CBS 125995ET | USA |
|
|
|
|
|
CPC 12795 | Polynesia |
|
|
|
|
|
CBS 126359 | USA |
|
|
|
|
|
CPC 10882 |
|
South Korea |
|
|
|
|
CPC 10538 | Mozambique |
|
|
|
|
|
DTO 323-C5 | Indoor air | China |
|
|
|
|
CPC 13252 | Rock | Australia |
|
|
|
|
CPC 13732 |
|
Laos |
|
|
|
|
CPC 14196 | Laos |
|
|
|
|
|
CPC 11609T | Man, bronchoalveolar lavage fluid | USA |
|
|
|
|
CBS 126364 |
|
Australia |
|
|
|
|
CBS 125997T | Russia |
|
|
|
|
|
CBS 189.54T | Man, mycosis | Brazil |
|
|
|
|
CBS 797.97T | Indoor environment | Netherlands |
|
|
|
The Maximum Likelihood (
Morphological and phylogenetically related species were analyzed using the genealogical consistency phylogenetic species identification (
DNA sequences used in this study (Table
Maximum Likelihood (RAxML) tree from the combined analysis of
The DNA base differences between our strains and related taxa in the three gene regions. Asterisks (*) denote our material.
Species | Strain number | Gene region and alignment positions | ||
---|---|---|---|---|
– | – | – | ||
0 | 3 | 0 | ||
|
MFLUCC 18-1559T | 0 | 29 | n/a |
|
MFLUCC 18-1557 | 0 | 29 | n/a |
|
CBS 112388 | 0 | 18 | 13 |
|
CBS 113738 | 0 | 16 | 13 |
|
CBS 101367 | 1 | 16 | 13 |
|
|
|
||
|
CBS 125995ET | – | – | – |
0 | 3 | 5 | ||
0 | 15 | n/a | ||
0 | 2 | 1 | ||
1 | 9 | 3 | ||
|
|
|
||
– | – | – | ||
|
CBS 125996T | 0 | 26 | 7 |
|
CPC 13870 | 0 | 22 | 7 |
|
DTO 323-E4 | 0 | 13 | 5 |
|
CPC 12216 | 0 | 2 | 5 |
|
CPC 14247 | 0 | 2 | 5 |
|
|
|||
– | – | – | ||
0 | 3 | 2 | ||
|
CBS 112388 | 1 | 29 | 14 |
|
CBS 113738 | 1 | 27 | 14 |
|
CBS 101367 | 2 | 27 | 14 |
The parameters of
Strain number |
|
Bayesian | |||||||
---|---|---|---|---|---|---|---|---|---|
|
PT |
|
|
|
|
Model | Unique site patterns | ASDSF | |
400 | 300 | 0.7475 | 0.8648 | 0.6464 | 0.2525 | Division 1 = 54 Division 2 = 99 Division 3 = 154 | 0.009875 | ||
281 | 2 | 0.8505 | 0.8817 | 0.7499 | 0.1495 | Division 1 = 30 Division 2 = 75 Division 3 = 128 | 0.009961 |
Maximum Likelihood (RAxML) tree from the combined analysis of
The pairwise homoplasy index (
In this section, we introduced two new species and report two new substrate records.
China, Guizhou Province, Guiyang, Huaxi district, plantation forest of
Saprobic on fallen leaves of
guizhouense , in reference to the type location (Guizhou Province), where the fungus was isolated.
China, Guizhou Province, Guiyang, Huaxi district, plantation forest of
Saprobic on fallen leaves of
China, Guizhou Province, Guiyang, Huaxi district, plantation forest of
Saprobic on fallen leaves of
China, Guizhou Province, Guiyang, Huaxi district, plantation forest of
Saprobic on fallen leaves of
In this paper, we revealed four
Sequences retrieved from
Our five strains pertain to two known species, viz.,
This research was supported by the following sources: The Open Project of Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education) (MOELP-201801), and Postgraduate Education Innovation Program in Guizhou Province (YJSKYJJ [2021] 058). We thank Profs. Mohammed Amin Uddin Mridha and Eric HC McKenzie for improving the quality of our manuscript.