Corresponding authors: Bo Zhang (
Academic editor: Bao-Kai Cui
In this contribution to the genus
Zhao G-P, Hu J-J, Tuo Y-L, Li Y, Zhang B (2022) Two new species of
In China, species diversity, taxonomy and phylogeny of macrofungi have been investigated in recent years, many new species having been discovered (
During a recent survey of macrofungi in northern China, we discovered some exciting and novel species of
This paper is principally based on materials collected by the senior author and collaborators over the past four years in northern China and specimens have been deposited at the
Photographs and descriptions of macroscopic morphological characteristics were made from fresh materials in the field. The colours correspond to the “Flora of British fungi: colour identification chart” (Royal Botanic Garden 1969). Collections were dried in an oven at 45 °C and rehydrated with 95% alcohol. All microscopic observations and measurements were made in ammoniacal Congo red with a 5% aqueous potassium hydroxide (
Genomic DNA was extracted from dried or fresh material stored in desiccant silica gel. The extraction method followed the nuclear Plant Genomic DNA Kit (Kangwei Century Biotechnology Company Limited, Beijing, China). The amplified segments were the nr
This study is based on around 25 specimens, including two outgroups (
Information on the specimens that were used in the phylogenetic analyses. Sequences that were newly generated in this study are indicated in black bold. T: Type.
Taxon | Voucher number | Country | GenBank accession number | References | |
---|---|---|---|---|---|
nr |
|||||
|
HGASMF013581 (T) | China |
|
– |
|
|
HGASMF0110046 | China |
|
|
|
|
CAL1624 (T) | India |
|
– |
|
|
TH9232 (T) | Guyana |
|
– |
|
|
MCA1313 | Guyana |
|
– |
|
|
MCA1070 | Guyana |
|
– |
|
|
TH9203 | Guyana |
|
– |
|
|
Yuan13878 (T) | China |
|
– |
|
|
Yuan 14776 (T) | China |
|
|
|
|
Yuan 14779 | China |
|
|
|
|
MH17001 | USA |
|
– | Genbank |
|
TH9075 (T) | Guyana |
|
– |
|
|
TH8999 | Guyana |
|
– |
|
|
TH9264 | Guyana |
|
– |
|
|
|
|
|
|
|
|
|
|
– |
|
|
|
HbO53302 | Norway |
|
– |
|
|
UPSF11801 | USA |
|
– |
|
|
MCA3107 | Guyana |
|
– |
|
|
TH7515 | Guyana |
|
– |
|
|
AFTOL286 | USA |
|
– | Genbank |
|
UPSF11794 | USA |
|
– |
|
|
PUN3884 (T) | India |
|
– |
|
|
MSR7 | India |
|
|
|
|
MSR8 | India |
|
|
|
|
CAL 1625 (T) | India |
|
– |
|
|
UPSF11789 | Sweden |
|
– |
|
|
UPSF11790 | Sweden |
|
– |
|
|
UPSF11791 | Spain |
|
– |
|
|
GDGM46432 | China |
|
– |
|
|
GDGM48105 (T) | China |
|
– |
|
|
GDGM49495 | China |
|
– |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
UPSF11794 | USA |
|
– |
|
|
TH9205 (T) | Guyana |
|
– |
|
|
MCA3186 | Guyana |
|
– |
|
|
CAL1533 (T) | India |
|
– |
|
|
CAL1534 | India |
|
– |
|
|
TH9220 (T) | Guyana |
|
– |
|
|
MCA3124 | Guyana |
|
– |
|
|
MAC1750 | Guyana |
|
– |
|
|
TH9204 (T) | Guyana |
|
– |
|
|
UPSF11793 | Sweden |
|
– |
|
|
BB 07.293 | Slovakia |
|
|
|
|
BIO 10986 (T) | Sweden |
|
|
|
|
FD3281 | Switzerland |
|
– | Genbank |
Sequences were assembled and edited using the software package Sequencher 5.4.6 (Gene Codes Corp., USA). Alignment of sequence data was performed with MUSCLE in MEGA 7.0.21 (
Seven new DNA sequences (3 nr
China. Jilin Province, Jilin City, Jiaohe County, Lafashan National Forest Part, Red Leaves Canyon, alt. 802.5 m,
Differs from other
Microscopic characteristics of
Scattered or gregarious in small clusters on the ground in coniferous and angiosperm mixed forests.
China. Jilin Province, Baishan City, Fusong County, Quanyang Town, alt. 780.4 m,
Differs from other
Microscopic characteristics of
Scattered to gregarious on the ground in a coniferous and angiosperm mixed forest.
1 | Clamp connections present |
|
– | Clamp connections absent |
|
2 | Stipe golden yellow to orangish-yellow |
|
– | Stipe grey |
|
3 | Pileus perforate to the base of the stipe |
|
– | Pileus not perforate |
|
4 | Basidiomata not brown, absolutely light colour |
|
– | Basidiomata greyish-brown to blackish-brown |
|
5 | Pileus white |
|
– | Pileus yellow |
|
6 | Pileus very small, usually < 10 mm in diam |
|
– | Pileus large, usually > 90 mm in diam |
|
7 | Basidiospores 10–15 μm long, average length > 11 µm |
|
– | Basidiospores 6–12 μm long, average length < 11 µm |
|
8 | Hymenophore smooth to slightly folded |
|
– | Hymenophore with well-developed veins or gill-folds |
|
9 | Basidia long, up to 106 µm long |
|
– | Basidia short |
|
10 | Basidia with 2 sterigmata |
|
– | Basidia with 2–4 sterigmata |
|
11 | Basidiospores broad, up to 11.5 μm wide |
|
– | Basidiospores 2–4 μm wide |
|
12 | Pileus surface smooth |
|
– | Pileus surface often with darker brown raised scales |
|
13 | Margin dirty white |
|
– | Margin not dirty white |
|
In our study, two new species formed a sister relationship with
However,
This study is funded by the National Natural Science Foundation of China (No. 31970020), the Scientific and Technological Tackling Plan for the Key Fields of Xinjiang Production and Construction Corps (No. 2021AB004), the National Key R & D of the Ministry of Science and Technology (2019YFD1001905-33), Research on the creation of excellent edible mushroom resources and high quality & efficient ecological cultivation technology in Jiangxi Province (20212BBF61002) and the National Key R & D of Ministry of Science and Technology (2018YFE0107800).