Corresponding author: Jiwen Xia (
Academic editor: Nalin Wijayawardene
Species of
Zhang Z, Liu R, Liu S, Mu T, Zhang X, Xia J (2022) Morphological and phylogenetic analyses reveal two new species of Sporocadaceae from Hainan, China. MycoKeys 88: 171–192.
The family
To date, most phylogenetic studies addressing genera of
The samples were collected from Hainan Province, China. The strains were isolated from diseased leaves of
Genomic DNA was extracted from fungal mycelium grown on
PCR was performed using an Eppendorf Master Thermocycler (Hamburg, Germany). Amplification reactions were performed in a 50 μL reaction volume, which contained 25 μL Green Taq Mix (Vazyme, Nanjing, China), 2 μL of each forward and reverse primer (10 μM) (Tsingke, Beijing, China), and 2 μL template genomic DNA, to which distilled deionized water was added. PCR parameters were as follows: 94 °C for 5 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at a suitable temperature for 30 s, extension at 72 °C for 1 min and a final elongation step at 72 °C for 7 min. Annealing temperature was 55 °C for
Newly generated sequences in this study were aligned with additional related sequences downloaded from GenBank (Table
Species and GenBank accession numbers of DNA sequences used in this study. New sequences are in bold.
Species | Strain | Host/substrate | Country | GenBank accession number | Reference | ||
---|---|---|---|---|---|---|---|
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CBS 122705 T |
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Italy |
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MFLUCC 14-0984 T |
|
Thailand |
|
– |
|
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MFLUCC 12-0310 |
|
Thailand |
|
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CFCC 54354 = SM9-1 T |
|
China |
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SM9-2 |
|
China |
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NBRC 9980 |
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Japan |
|
– | – |
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KUMCC 15-0520 T | China |
|
– | – |
|
|
CBS 101009 | Air | Japan |
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|
|
|
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CBS 143391 T |
|
Germany |
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PSHI2004Endo1030 |
|
China |
|
– |
|
Liu et al. 2006 |
PSHI2004Endo1031 |
|
China |
|
– |
|
Liu et al. 2006 | |
|
CBS 546.80 | Culture contaminant | Netherlands |
|
|
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CBS 199.82 T |
|
Italy |
|
– |
|
|
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CBS 115004 |
|
Netherlands |
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CBS 144034 T |
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Mexico |
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HKAS 10065 T | China |
|
– |
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MFLUCC 17-1754 T |
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Thailand |
|
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MFLUCC 17-2544 T |
|
Thailand |
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CBS 367.54 T | Canvas | New Zealand |
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MFLUCC 12-0286 T | Unidentified tree | China |
|
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CFCC 54339 = SM32 |
|
China |
|
|
|
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MFLUCC 17-1555 T |
|
Thailand |
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COAD 2166 T |
|
Brazil |
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CFCC 54341 = ZY4 |
|
China |
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ZY4-2D |
|
China |
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MFLUCC 18-0113 T | Dead leaves | Thailand | – |
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MFLUCC 12-0261 T | China |
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MFLUCC 12-0281 T | China |
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MFLUCC 15-0152 T |
|
Thailand |
|
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– |
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HGUP 4019 T |
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China |
|
|
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CBS 600.96 T | Leaf litter | Cuba |
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MFLUCC 14-0106 T |
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Chiang Rai, Thailand |
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|
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CBS 140162 T |
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Egypt |
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MFLUCC 12-0283 T | Dead plant materials | China |
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CBS 264.37 T |
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– |
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CGMCC 3.9123 T | Mangrove plant | China |
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CBS 362.72 T | Dead ant | Ghana |
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CBS 115.83 | Plant debris | Cuba |
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COAD 2637 T |
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Minas Gerais, Brazil |
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CBS 114495 T | USA |
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CBS 137768 T |
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Iran |
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Ayoubi et al. 2016 |
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CBS 257.31 T |
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Indonesia |
|
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BRIP 63737c T |
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Australia |
|
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MFLUCC 12-0652 T | France |
|
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CBS 336.86 T |
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Iraq |
|
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MFLUCC 15-0776 T | Thailand |
|
|
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CBS 138.41 T |
|
South Africa |
|
|
|
|
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KUMCC 17-0175 T |
|
China | – |
|
|
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URM 7148-01 T |
|
Brazil |
|
|
– |
|
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MFLUCC 17-1738 T |
|
Thailand |
|
|
|
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MFLUCC 18-0119 T |
|
Thailand |
|
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CBS 394.48 T | UK |
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CBS 254.32 |
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Indonesia |
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CBS 114178 T | Zimbabwe |
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MFLUCC 17-1550 T |
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Thailand |
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CBS 124745 |
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USA |
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CBS 101057 T | New Zealand |
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||
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CFCC 51992 T |
|
China |
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CFCC 51993 |
|
China |
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|
N |
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MFLUCC 12-0233 T |
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Thailand |
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MFLUCC 12-0282 T | China |
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|
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CFCC 54338 = SM15-1 T |
|
China |
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MFLUCC 17-1745 T |
|
Thailand |
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IMI 192475 T |
|
Australia |
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CBS 450.74 T | soil under |
Suriname |
|
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MFLUCC 17-1730 T |
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Thailand |
|
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MFLUCC 12-0285 T | unidentified plant | China |
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MFLUCC 15-1265 T | China |
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CBS 111495 T | Zimbabwe |
|
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CBS 116061 T |
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Italy |
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CBS 270.82 |
|
Italy |
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CBS 114126 T | New Zealand |
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CBS 114193 T | Australia |
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CBS 114127 T | Australia |
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CBS 265.33 T |
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The Netherlands |
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CBS 442.67 T | Kenya |
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CBS 114138 T | New Zealand |
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HGUP4057 T |
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China |
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CBS 353.69 T |
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Denmark |
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CBS 278.35 |
|
– |
|
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CBS 393.48 T | – | Portugal |
|
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CBS 144035 T |
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Australia |
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CBS 272.29 T |
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Indonesia |
|
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CBS 413.62 T |
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Nigeria |
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CBS 459.78 T |
|
India |
|
|
|
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CBS 340.97 T |
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Australia |
|
|
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CBS 133587 T |
|
Tristan da Cunha |
|
|
|
|
Isolates marked with “T” are ex-type or ex-epitype strains.
Nine strains of
Phylogram of
Bayesian posterior probability (≥ 0.90) and
China, Hainan Province: East Harbour National Nature Reserve, on diseased leaves of
Name refers to the genus of the host plant
Leaf spots irregular, pale brown in centre, brown to tan at margin. Sexual morph not observed. Asexual morph on
Colonies on
China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of
China, Hainan Province, Haikou City: East Harbour National Nature Reserve, on diseased leaves of
Named after the host location, Haikou City.
Leaf spots irregular, grey white in centre, brown to tan at margin. Sexual morph not observed. Asexual morph on
Colonies on
China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of
Phylogenetic analysis of a combined three-gene
Leaf spots irregular, pale brown in centre, brown to tan at margin. Asexual morph on
Colonies on
China, Hainan Province: Five Fingers Group Scenic Area, 20 May 2021, Z.X. Zhang. On diseased leaves of
In the present study, two strains (SAUCC210112 and SAUCC210113) from symptomatic leaves of
Leaf spots irregular, pale brown in centre, brown to tan at margin. Asexual morph on
Colonies on
China, Hainan Province: East Harbour National Nature Reserve, 23 May 2021, Z.X. Zhang. On diseased leaves of
In the present study, two strains (SAUCC210087 and SAUCC210088) from symptomatic leaves of
Based on phylogeny and morphology, nine strains from three host species (
As many pestalotioid species have overlapping morphological traits, sequence data is essential to resolve these three genera and introduce new species (
This work was jointly supported by the National Natural Science Foundation of China (nos. 31900014, U2002203, 31750001) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100704).
The combined
phylogenetic
The combined