Corresponding author: ChengLin Hou (
Academic editor: María P. Martín
Three new species,
Zhou H, Cheng GQ, Sun XM, Cheng RY, Zhang HL, Dong YM, Hou CL (2022) Three new species of
In previous studies of
Currently, there are 25 recognised species in
Yanshan Mountains are located in North China and have a warm temperate continental monsoon climate, with higher plant diversity. The dominant plants include
Collections were obtained and photographed in the field from Yanshan Mountains in China from 2017 to 2020. The collected specimens were dehydrated with a dryer (Dorrex) at 50 °C and the specimens were deposited in the
DNA extraction was achieved by the M5 Plant Genomic DNA Kit [Mei5 Biotechnology, Co., Ltd, China]. The purified DNA was dissolved in 1 × TE buffer and stored at – 20 °C for later use. The PCR amplifications were performed in Bio-Rad S1000 TM Thermal Cycler [Bio-Rad Laboratories, Inc, USA]. The primer sets ITS1/ITS4 (
The generated raw reads of the DNA sequences were used to obtain consensus sequences using SeqMan v.7.1.0 in the DNASTAR Lasergene Core Suite software (DNASTAR Inc., Madison, WI, USA). All sequences were aligned using MAFFT v.6 (
Sequences information used in the phylogenetic analysis in this study.
Taxa | Voucher | Locality | ITS | LSU |
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GLM-F116094 | Kenya |
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– | – | – |
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DED8340 | Sao Tome |
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– | – | – |
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79478 (TAA) Type | Turkmenistan |
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– | – |
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SZMC-NL-2347 | – |
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SFSU DED 8339 | Sao Tome |
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– | – | – |
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FP1R4 | USA |
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– | – | – |
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MP2R2 | USA |
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– | – | – |
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LAS73030 Neotype | Sweden |
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– | – |
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CLUF302 Type | Italy |
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Pegler2133 (K) | Sri Lanka |
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– | – | – |
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GLM-F126263 Type | Germany |
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– | – |
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LÖ138-01 (UPS) | Sweden |
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Sharp20863 (MICH) Type | USA |
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– | – |
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HMJAU5148 | China: Jilin |
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UES2918 Type | Mexico |
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– |
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HMJUA37867 | China: Jilin |
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HMJAU37877 | China: Chongqing |
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HMJAU37807 Type | China: Henan |
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HMJAU37808 | China: Henan |
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HFJAU1014 | China: Jiangxi |
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– | – | – |
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HFJAU1274 | China: Jiangxi |
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– | – | – |
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HFJAU1488 | China:Anhui |
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– | – | – |
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– | – |
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HMJAU37801 Type | China: Hubei |
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HMJAU37916 | China: Henan |
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HMJAU37811 Type | China: Jilin |
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HMJAU37913 | China: Jilin |
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GB:LO55-12 | – |
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– | – |
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HFJAU1515 | China: Fujian |
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– |
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Chiarello 07-10-2013 | – |
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– | – | – |
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TL9035 (C) | Ecuador |
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– |
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ADK4162 (BR) | Togo |
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– | – |
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BAB-4773 | India |
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– | – | – | |
BAB-5172 | India |
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– | – | – | |
BAB-4748 | India |
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– | – | – | |
BAB-4747 | India |
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– | – | – | |
BAB-5202 | India |
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– | – | – | |
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LÖ237-04 | Sweden |
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Notes: The new generated sequences are emphasised in bold.
Maximum Parsimony analysis was performed by a heuristic search option of 1000 random-addition sequences with a tree bisection and reconnection (
Maximum Likelihood analysis was performed with a GTR site substitution model (
The combined alignment and phylogenetic tree were submitted on TreeBASE (
For the ITS-LSU-
Multi-gene phylogenetic tree obtained from the Bayesian analysis. Numbers above branches are Bayesian posterior probability (pp) values, Maximum Likelihood bootstrap (MLB) and Maximum parsimony bootstrap (
Based on the results, six specimens were assigned to three branches and were described as three new species. The three new species (
China, Beijing, Changping District, Beitaizi Village,
Basidiomata and microscopic features of
Pileus 20–60 mm, flabellate, flattening with age, hygrophanous, slightly dirty white (#e3dac9) to pale brown (#deb887). Veil white (#ffffff), fibrils in young, evanescent. Context 1.0–2.0 mm broad at centre, same colour as pileus. Lamellae sparsely to moderately, adnate, slightly dirty white (#e3dac9) to champagne (#fad6a5), edge white (#ffffff) as spores mature. Stipes 50–130 × 3–6 mm, smooth, fibrils on the base, cornsilk (#f0ead6) to white (#ffffff).
Basidiospores 5.8–8.2 × 3.3–5.4 μm, Q = 1.4–2.0, ellipsoid to long ellipsoid, ovoid to ellipsoid, partly triangular at base, dark brown (#b8860b) to brown (#b06500) in water, smooth, abundant, multi-guttules, often with germ pore. Basidia 17–31 × 5.8–7.5 μm, short clavate, hyaline, 4-spored. Cheilocystidia 22–35 (40) × 8–11 (15) μm, irregular utriform or claviform, apex obtuse or broadly obtuse or often subcapitate, rarely with deposits. Pileipellis consists of 2–3 cells deep layer of irregular subglobose cell, irregular oval, (18) 20–27μm broad.
. Clumped on the ground with rich humus in broad-leaved forests or broad-leaved shrubs.
China, Beijing, Changping District, Tailing,
Basidiomata and microscopic features of
China, Tianjin, Jizhou District, Sanjiebei, 40.227984°N, 117.43354°E, alt. 235 m, 17 Aug 2019, coll. X.Y. Shen, H. Zhou and R.T. Zhang,
Pileus 5–20 mm, campanulate to conical, smooth, fibrils in young, evanescent, brown (#b06500) to golden brown (#996515). Veil white (#ffffff), fibrils in young, evanescent. Context 0.2–0.5 mm broad at centre, same colour as pileus. Lamellae moderately to normally, adnate, slightly dirty white (#e3dac9) to white (#ffffff), edge white (#ffffff) as spores mature. Stipes 20–60 × 1–3 mm, smooth, fibrils on the base, cornsilk (#f0ead6) to white (#ffffff).
Basidiospores 5.5–6.7 × 3.2–4.5 μm, Q = 1.4–2.0, ellipsoid to ovoid, pale cream (#fffff0) to pale lemon (#fffacd) in water, smooth, multi-guttules, germ pore absent. Basidia 18–27 × 5–10 μm, short clavate, hyaline, 4-spored. Cheilocystidia 21–28 (30) × 8–12 (15) μm, utriform or claviform, apex obtuse or broadly obtuse or often subcapitate, rarely with deposits. Trama of gills irregular. Pileipellis consists of irregular subglobose cell, irregular oval or long oval, (13) 16–25 μm broad.
Clumped on the ground with rich humus in broad-leaved forests or broad-leaved shrubs.
China, Tianjin, Jizhou District, Huangyaguan Great Wall,
China, Beijing, Changping District, Sidaohe Village,
Basidiomata and microscopic features of
Pileus 5–25 mm, hemispherical to conical, hygrophanous,
Basidiospores 6.0–7.0 × 3.2–4.5 μm, Q = 1.4–1.9, ellipsoid, floral white (#fffaf0) to dark yellow (#eedc82) in water, smooth, abundant, multi-guttules, germ pore absent. Basidia 15–20 × 5–8 μm, short clavate, hyaline, 4-spored. Cheilocystidia 17–27 (31) × 7–11 (13) μm, utriform, apex obtuse or broadly obtuse or often subcapitate, rarely with deposits. Trama of gills irregular. Pileipellis consisted of irregular subglobose cell, irregular oval or long oval, (22) 25–32 μm broad.
Clumped on the ground with rich humus in deciduous broad-leaved or deciduous coniferous forests.
China, Beijing, Yanqing District, Yudu Mountain,
In this study, three new species were identified by morphology and phylogeny. It is very interesting that the three new species
Except for morphological differences, the three new species in this study can also be distinguished by sequence similarity.
According to the research of
It is considered that the natural growth of
This study was supported by the Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment, China. (2019HJ2096001006) and National Natural Science Foundation of China (No. 31870629).