The most common substrates associated with the top 50 most wanted fungi at the phylum, class, and order level. Each fungal sequence was assigned to substrate according to its GenBank entry (or underlying publication as applicable). The major substrates included soil (light blue), living plants (blue), mycorrhiza (orange), dust (green), lichen (dark blue), dead wood (red), and other (grey). To improve readability, rare substrates (<3 occurrences) were merged into the ‘other’ category.