Corresponding author: Yong Li (
Academic editor: N Wijayawardene
Jiang N, Voglmayr H, Piao C-G, Li Y (2021) Two new species of
Species of
In the present study,
The fresh specimens of cankered branches were sampled from
Genomic DNA was extracted from colonies grown on cellophane-covered
Genes used in this study with
|
|
|
|
|
ITS1/ITS4 | (95 °C: 30 s, |
|
|
CAL228F/CAL737R | (95 °C: 15 s, |
|
|
CYLH3F/H3-1b | (95 °C: 30 s, |
|
|
EF1-728F/EF1-986R | (95 °C: 15 s, |
|
|
T1(Bt2a)/Bt2b | (95 °C: 30 s, |
Glass & Donaldson 1995; |
The quality of the amplified nucleotide sequences was checked and the sequences assembled using SeqMan v.7.1.0. Reference sequences were retrieved from the National Center for Biotechnology Information (
The phylogenetic analyses of the combined gene regions were performed using Maximum Likelihood (
Isolates and GenBank accession numbers used in the phylogenetic analyses of
Species | Strain | Host | Origin | GenBank accession numbers | ||||
---|---|---|---|---|---|---|---|---|
|
|
|
|
|
||||
|
CBS 129521 |
|
Australia |
|
|
|
|
|
|
MFLUCC 17-0956 |
|
Italy |
|
|
NA |
|
|
|
|
China |
|
|
|
|
NA | |
|
|
China |
|
|
|
|
NA | |
|
PSCG 047 |
|
China |
|
|
|
|
|
|
LC6161 |
|
China |
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
CBS 495.72 |
|
Canada |
|
|
|
|
|
|
CBS 114015 |
|
South Africa |
|
|
|
|
|
|
STE-U 2660 |
|
France | NA |
|
NA |
|
NA |
|
CBS 126679 |
|
Portugal |
|
|
|
|
|
|
CBS 720.97 |
|
East Africa |
|
|
|
|
|
|
CBS 111592 |
|
Austria |
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
IFRDCC 3051 |
|
China |
|
NA | NA | NA | NA |
|
DP0482 |
|
Austria |
|
|
|
|
|
|
CBS 161.64 |
|
India |
|
|
|
|
|
|
CBS 114979 |
|
Hong Kong |
|
|
|
|
|
|
MFLUCC 12-0299a | Unknown | Thailand |
|
|
NA |
|
|
|
CBS 136967 |
|
Chile |
|
|
NA |
|
|
|
CBS 117169 |
|
South Africa |
|
|
|
|
|
|
CBS 111886 |
|
Australia |
|
|
|
|
|
|
CBS 146457 |
|
Australia |
|
NA | NA |
|
|
|
CBS 136972 |
|
Italy |
|
|
|
|
|
|
CBS 122.21 |
|
USA |
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
BRIP 54792 |
|
Australia |
|
NA | NA |
|
|
|
SBen914 |
|
USA |
|
|
NA |
|
NA |
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China | KX0246554 |
|
|
KX0246556 |
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
ZJUD62 |
|
China |
|
NA |
|
|
|
|
ZJUD47 |
|
China |
|
NA |
|
|
|
|
CBS 143347 |
|
Czech Republic |
|
|
|
|
|
|
CBS 133183 |
|
Brazil |
|
|
|
|
|
|
URM7485 |
|
Brazil |
|
|
NA |
|
|
|
SAUCC194.92 |
|
China |
|
|
|
|
|
|
CPC 19740 |
|
South Africa |
|
NA | NA | NA | NA |
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
CPC 21916 |
|
South Africa |
|
NA | NA |
|
NA |
|
CBS 127268 |
|
Croatia |
|
|
|
|
|
|
|
China |
|
|
|
NA |
|
|
|
|
China |
|
|
|
NA |
|
|
|
CBS 454.81 |
|
Greece |
|
|
|
|
|
|
BRIP 54884m |
|
Australia |
|
NA | NA |
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
PSCG 435 |
|
China |
|
|
|
|
|
|
SAUCC194.35 |
|
China |
|
|
|
|
|
|
MFLUCC 17-1023 |
|
Italy |
|
|
NA |
|
|
|
|
China |
|
NA |
|
|
|
|
|
|
China |
|
NA |
|
|
|
|
|
CPC 27302 |
|
South Africa |
|
NA |
|
NA |
|
|
AR3405 |
|
USA |
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
CGMCC 3.15224 |
|
China |
|
|
|
|
|
|
CGMCC 3.15225 |
|
China |
|
|
NA |
|
NA |
|
MFLU 17-2770 |
|
Thailand |
|
|
NA |
|
|
|
LC3083 |
|
China |
|
NA |
|
|
NA |
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
CGMCC 3.20096 | Unknown | China |
|
|
|
|
|
|
CBS 124654 |
|
Turkey |
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
CBS 162.33 |
|
USA |
|
|
|
|
|
|
CAA 823 |
|
Portugal |
|
|
|
|
|
|
DAOM 42078 |
|
Canada |
|
NA |
|
|
|
|
CBS 117499 |
|
South Africa |
|
|
|
|
|
|
CBS 122676 |
|
South Africa |
|
|
|
|
|
|
FAU461 |
|
Italy |
|
|
NA |
|
|
|
CPC 21169 |
|
South Africa |
|
NA | NA | NA | NA |
|
ZJUD89 |
|
China |
|
NA |
|
|
|
|
MFLUCC 17-1015 |
|
Italy |
|
NA | NA |
|
|
|
CBS 146453 |
|
Australia |
|
NA | NA |
|
|
|
LC4802 |
|
China |
|
|
|
|
|
|
CGMCC 3.17084 |
|
China |
|
NA | NA |
|
|
|
CBS 133811 |
|
Brazil |
|
|
|
|
|
|
CBS 146.46 |
|
Netherlands |
|
|
|
|
|
|
CBS 121004 |
|
USA |
|
|
|
|
|
|
CGMCC 3.17081 |
|
China |
|
NA | NA |
|
|
|
|
China |
|
|
|
|
|
|
|
CBS 139.27 |
|
USA |
|
|
|
|
|
|
CBS 143349 |
|
United Kingdom |
|
|
|
|
|
|
AR5193 |
|
Germany |
|
|
|
|
|
|
|
China |
|
NA |
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
NA |
|
|
NA | |
|
|
China |
|
NA |
|
|
|
|
|
|
China |
|
NA |
|
|
|
|
|
MAFF 625034 |
|
Japan | NA |
|
|
NA |
|
|
AR5211 |
|
France |
|
|
|
|
|
|
CGMCC 3.17089 |
|
China |
|
NA | NA |
|
|
|
MFLUCC 17-0963 |
|
Italy |
|
|
NA |
|
|
|
DAOM 695742 |
|
Canada |
|
NA | NA |
|
|
|
MFLUCC 16-0113 |
|
China |
|
NA |
|
|
|
|
CBS 144.27 |
|
USA |
|
|
|
|
|
|
CBS 587.79 |
|
Japan |
|
|
|
|
|
|
CBS 338.89 |
|
Yugoslavia |
|
|
|
|
|
|
MFLU 17-0646 |
|
United Kingdom |
|
|
NA |
|
|
|
CBS 132525 |
|
China |
|
NA | NA | NA | NA |
|
CBS 111553 |
|
Spain |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
BRIP 54781 |
|
Australia |
|
|
NA |
|
NA |
|
|
China |
|
|
NA |
|
NA | |
|
|
China |
|
|
NA |
|
NA | |
|
PSCG 051 |
|
China |
|
|
|
|
|
|
CGMCC 3.17087 |
|
China |
|
|
NA |
|
|
|
CBS 180.91 |
|
USA |
|
|
|
|
|
|
Unknown | China |
|
|
|
|
|
|
|
Unknown | China |
|
|
|
|
|
|
|
MFLUCC 12-0542a | Unknown | Thailand |
|
|
NA |
|
|
|
BRIP 55657a |
|
Australia |
|
NA | NA |
|
|
|
SAUCC194.84 |
|
China |
|
|
|
|
|
|
JZB320087 |
|
China |
|
|
NA |
|
|
|
BRIP 54025 |
|
Australia | NA | NA | NA |
|
|
|
CGMCC 3.20100 | Unknown | China |
|
|
|
|
|
|
CBS 592.81 |
|
Serbia |
|
|
|
|
|
|
SAUCC194.77 |
|
China |
|
|
|
|
|
|
CPC 26215 |
|
France |
|
|
|
|
|
|
SAUCC194.85 |
|
China |
|
|
|
|
|
|
CBS 145.26 |
|
USA |
|
|
NA |
|
|
|
CBS 143351 |
|
Spain |
|
|
|
|
|
|
CBS 115448 |
|
China |
|
|
|
|
|
|
JZB320123 |
|
China |
|
|
NA |
|
|
|
LC6754 |
|
China |
|
|
|
|
|
|
CBS 133813 |
|
Brazil | NA |
|
|
|
|
|
CBS 133812 |
|
Brazil |
|
|
|
|
|
|
CGMCC 3.20092 | Unknown | China |
|
|
NA |
|
|
|
CPC 22549 |
|
Zambia |
|
NA | NA | NA |
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
BRIP 54033 |
|
Australia | NA | NA | NA |
|
NA |
|
BRIP 54031 |
|
Australia | NA | NA | NA | NA |
|
|
CGMCC 3.20101 | Unknown | China |
|
|
|
|
|
|
BRIP 54900 |
|
Australia |
|
NA | NA |
|
NA |
|
SAUCC194.22 |
|
China |
|
|
|
|
|
|
CGMCC 3.15175 |
|
China |
|
|
NA |
|
|
|
FAU599 |
|
USA |
|
|
|
|
|
|
CBS 194.36 |
|
Canada |
|
|
|
|
|
|
CBS 123212 |
|
Portugal |
|
|
|
|
|
|
SAUCC194.36 |
|
China |
|
|
|
|
|
|
CBS 146455 |
|
Australia |
|
NA | NA |
|
|
|
BRIP 55064a |
|
Australia |
|
NA | NA |
|
|
|
CGMCC 3.15181 |
|
China |
|
NA | NA |
|
|
|
CAA 734 |
|
Portugal |
|
|
|
|
|
|
BRIP 54256 |
|
Australia |
|
NA | NA |
|
|
|
CBS 133185 |
|
Brazil |
|
|
|
|
|
|
CPC 21896 |
|
South Africa |
|
NA | NA | NA |
|
|
SAUCC194.111 |
|
China |
|
|
|
|
|
|
SAUCC194.55 |
|
China |
|
|
|
|
|
|
CBS 435.87 |
|
Indonesia |
|
|
|
|
|
|
BRIP 54884e |
|
Australia |
|
NA | NA |
|
|
|
CGMCC 3.20097 | Unknown | China |
|
|
|
|
|
|
CGMCC 3.20098 | Unknown | China |
|
|
|
|
|
|
BRIP 54736j |
|
Australia |
|
NA | NA |
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
CBS 129519 |
|
Australia |
|
|
|
|
|
|
CBS 109490 |
|
USA |
|
|
|
|
|
|
MFLUCC 14-1136 |
|
Thailand |
|
|
NA |
|
|
|
BRIP 54801 |
|
Australia |
|
NA | NA |
|
|
|
CBS 127269 |
|
Croatia |
|
|
|
|
|
|
CPC 26217 |
|
France |
|
NA | NA | NA |
|
|
LC3166 |
|
China |
|
NA |
|
|
|
|
ZJUD93 |
|
China |
|
NA |
|
|
|
|
CGMCC 3.17093 |
|
China |
|
|
NA |
|
|
|
CBS 133186 |
|
Brazil |
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
MFLUCC 17-0607 |
|
Thailand |
|
NA | NA | NA |
|
|
CBS 133184 |
|
Brazil |
|
|
|
|
|
|
CPC 22729 |
|
Zambia |
|
NA | NA | NA |
|
|
PSCG 035 |
|
China |
|
|
|
|
|
|
BRIP 54847 |
|
Australia |
|
NA | NA |
|
|
|
CPC 19183 |
|
Netherlands |
|
NA | NA | NA | NA |
|
CPC 27480 |
|
Malaysia |
|
NA |
|
NA |
|
|
LC3215 |
|
China |
|
NA | NA |
|
|
|
CBS 109745 |
|
Austria |
|
|
|
|
|
|
CBS 151.73 |
|
Netherlands |
|
|
NA | NA | NA |
|
MFLUCC 16-0105 |
|
China |
|
|
NA |
|
|
|
AR4203 |
|
USA |
|
|
|
|
|
|
CAA 817 |
|
Portugal |
|
|
|
|
|
|
LC6155 |
|
Japan |
|
|
|
|
|
|
SAUCC194.72 |
|
China |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
CBS 101339 |
|
Dominican Republic |
|
|
|
|
|
|
CBS 176.77 |
|
Iraq |
|
|
|
|
|
|
MFLU 15-3228 |
|
Italy |
|
|
NA |
|
|
|
CPC 21634 |
|
South Africa |
|
NA | NA |
|
|
|
CPC 21638 |
|
South Africa |
|
NA | NA | NA |
|
|
MFLUCC 10-0580a |
|
Thailand |
|
|
NA |
|
|
|
SAUCC194.112 |
|
China |
|
|
|
|
|
|
CAA483 |
|
Portugal |
|
|
|
|
|
|
CPC 26646 |
|
South Africa |
|
|
|
|
|
|
CBS 133182 |
|
Brazil |
|
|
|
|
|
|
MFLUCC 16-0997 |
|
Italy | NA | NA | NA |
|
NA |
|
CPC 18191 |
|
South Africa |
|
NA | NA | NA | NA |
|
MFLUCC 17-2658 |
|
United Kingdom |
|
|
NA | NA |
|
|
COAD 2914 |
|
Brazil |
|
|
NA |
|
NA |
|
CAA 762 |
|
Portugal |
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
AR3422 |
|
Austria |
|
|
NA |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
CBS 116311 |
|
South Africa |
|
|
|
|
|
|
BRIP 54669b |
|
Australia |
|
NA | NA |
|
|
|
BRIP 54825 |
|
Australia |
|
NA | NA |
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
CBS 133181 |
|
Brazil |
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
MFLU 15-1279 |
|
Italy |
|
|
NA |
|
|
|
CBS 296.67 |
|
Netherlands |
|
|
|
|
|
|
CBS 146456 |
|
Australia |
|
NA | NA | NA |
|
|
|
China |
|
|
NA |
|
|
|
|
|
China |
|
|
NA |
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
BRIP 55665a |
|
Australia |
|
NA | NA |
|
|
|
|
China |
|
|
|
|
NA | |
|
|
China |
|
|
|
|
NA | |
|
MFLUCC 10-0573a |
|
Thailand | NA |
|
NA |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
FAU635 |
|
USA |
|
|
|
|
|
|
CPC 24951 |
|
Spain |
|
NA |
|
NA |
|
|
PSCG 383 |
|
China |
|
|
|
|
|
|
CBS 136969 |
|
Italy |
|
|
|
|
|
|
CBS 370.54 |
|
Italy |
|
|
|
|
|
|
ZJUD95 |
|
China |
|
NA |
|
|
|
|
KUMCC 17-0151 | Unknown | China |
|
NA | NA |
|
|
|
KUMCC 17-0153 | Unknown | China |
|
NA | NA |
|
|
|
CBS 464.90 |
|
South Africa |
|
|
|
|
|
|
MFLUCC 16-0117 |
|
China |
|
NA | NA |
|
|
|
MFLUCC 12-0777 |
|
Thailand |
|
|
NA |
|
|
|
MFLUCC 13-0471 |
|
Thailand |
|
|
NA |
|
|
|
MFLUCC 12-0767 |
|
China |
|
|
|
|
|
|
CBS 133180 |
|
Brazil |
|
|
|
|
|
|
CGMCC 3.15183 |
|
China |
|
NA | NA |
|
NA |
|
MFLUCC 10-0576a |
|
Thailand |
|
|
NA |
|
NA |
|
MFLUCC 12-0033 |
|
Thailand |
|
NA | NA |
|
NA |
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
MFLUCC 17-1051 |
|
Italy |
|
|
NA |
|
|
|
CBS 534.93 |
|
Australia |
|
|
|
|
|
|
BRIP 62248a |
|
Australia |
|
NA | NA |
|
|
|
FAU656T |
|
USA |
|
|
|
|
|
|
|
China |
|
|
|
|
NA | |
|
|
China |
|
|
|
|
NA | |
|
LC6624 | Unknown | China |
|
NA |
|
|
|
|
ZJUD52 |
|
China |
|
NA |
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
CBS 160.32 |
|
USA |
|
|
|
|
|
|
CBS 137985 |
|
Zambia |
|
NA | NA | NA |
|
|
BRIP 57887a |
|
Australia |
|
NA | NA |
|
|
|
LC4421 |
|
China |
|
NA |
|
|
|
|
|
China |
|
|
|
|
|
|
|
|
China |
|
|
|
|
|
|
|
JZB320071 |
|
China |
|
|
NA |
|
|
|
CMW 40748 |
|
South Africa |
|
NA | NA | NA |
|
|
LC6707 |
|
China |
|
NA |
|
|
|
|
Unknown | China |
|
|
|
|
|
|
|
Unknown | China |
|
|
|
|
|
|
|
LC6168 | Unknown | China |
|
|
|
|
|
|
PSCG 031 |
|
China |
|
NA |
|
|
|
|
CBS 121124 |
|
NA |
|
|
|
|
|
Note: NA, not applicable. Strains in this study are marked in bold.
The five-gene sequence dataset (
Phylogram of
With reference to
Conidiomata pycnidial, discoid, immersed in bark, scattered, erumpent through the bark surface, with a solitary locule. Locule 800–1250 μm diam., undivided. Conidiophores 22–68.5 × 1.5–3 μm (av. = 39.8 × 2.2 μm, n = 50), cylindrical, attenuate towards the apex, hyaline, slightly brown at base, phialidic, unbranched, straight or slightly curved. Alpha conidia (5.8–)7.1–8.9(–11.2) × (1.5–)1.8–2.2(–2.7) μm (av. = 7.9 × 2.0 μm, n = 50), L/W = 3.2–4.7 (av. = 3.8, n = 50), hyaline, aseptate, subcylindrical with a nearly rounded apex, multi-guttulate, sometimes acute at both ends. Beta conidia not observed.
Colonies are initially white with fluffy aerial mycelium, becoming dirty white after 2 weeks, and conidiomata are randomly distributed with orange conidial drops oozing out of the ostioles.
NETHERLANDS. Utrecht City, on branches of
Name from “
Conidiomata pycnidial, conical, immersed in bark, scattered, erumpent through the bark surface, with a solitary locule. Locule 450–700 μm diam., undivided. Conidiophores 6.5–25 × 1.5–4 μm (av. = 15.4 × 2.4 μm, n = 50), cylindrical, attenuate towards the apex, hyaline, slightly brown, phialidic, unbranched, slightly curved. Alpha conidia (9.2–)10.1–12.3(–13.5) × (3.8–)4.2–4.9(–5.2) μm (av. = 11.5 × 4.5 μm, n = 50), L/W = 2.0–3.2 (av. = 2.5, n = 50), hyaline, aseptate, fusiform to oval, multi-guttulate, acute at both ends. Beta conidia not observed.
Colonies are initially white, aerial mycelium turning grey at edges of plate, yellowish pigmentation developing in centre, conidiomata not produced until 2 weeks.
NETHERLANDS. Utrecht City, on branches of
In this study, branch-inhabiting
Phylogenetic analyses were conducted based on a combined DNA sequence matrix of five loci (
The utility of host association for
This research was funded by the National Microbial Resource Center of the Ministry of Science and Technology of the People’s Republic of China (NMRC-2021-7).