Corresponding author: Guo Y. Zhou (
Academic editor: R. Phookamsak
Tea-oil tree (
Yang Q, Tang J, Zhou GY (2021) Characterization of
Tea-oil tree,
Diseases are a major constraint to
Species identification criteria in
The classification of
Leaves of
Agar plugs (6 mm diam.) were taken from the edge of actively growing cultures on
Genomic DNA was extracted from colonies grown on cellophane-covered
The quality of the amplified nucleotide sequences was checked and combined using SeqMan v.7.1.0 and reference sequences were retrieved from the National Center for Biotechnology Information (
The phylogenetic analyses of the combined gene regions were performed using Maximum Likelihood (
Isolates and GenBank accession numbers used in the phylogenetic analyses of
Species | Isolate | Host | Location | GenBank accession numbers | ||||
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ITS |
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MFLUCC 17-0956 |
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Italy |
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NA |
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CFCC 52554 |
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China |
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NA |
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CFCC 52556 |
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China |
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CBS 146.46 | Netherlands |
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CBS 126679 |
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Portugal |
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CBS 111592 |
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Austria |
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CGMCC 3.17533 |
|
China |
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NA | NA |
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CBS 161.64 |
|
India |
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CBS 114979 |
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Hong Kong |
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MFLUCC 12-0299 | Unknown dead leaf | Thailand |
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NA |
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CGMCC 3.17248 |
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China |
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NA |
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CFCC 52584 |
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China |
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CPC 28262 |
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Czech Republic |
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CBS 139.27 | USA |
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CFCC 52565 |
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China |
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BRIP 54884m |
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Australia |
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NA | NA |
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SAUCC194.35 |
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China |
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CFCC 52569 | China |
|
NA |
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CGMCC 3.15224 |
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China |
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CGMCC 3.15225 | China |
|
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NA |
|
NA | |
|
MFLU 17-2770 |
|
Thailand |
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NA |
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CFCC 52571 |
|
China |
|
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CBS 136.25 | Unknown |
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CBS 117499 |
|
South Africa |
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ZJUD89 |
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China |
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NA |
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BRIP 66524 | South Africa |
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NA | NA |
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CBS 133811 |
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Brazil |
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AR5193 | Germany |
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BRIP 54781 |
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Australia |
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NA | NA |
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CFCC 52582 |
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China |
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NA |
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NA |
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MAFF 246408 | Japan |
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CGMCC 3.17087 |
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China |
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NA |
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CFCC 53087 | Unknown | China |
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MFLUCC 12-0542a | Unknown dead leaf | Thailand |
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NA |
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China |
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NA |
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AR5211 |
|
France |
|
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SAUCC194.85 |
|
China |
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China |
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NA |
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CFCC 52586 |
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China |
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BRIP 54900 |
|
Australia |
|
NA | NA |
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MFLUCC 17-0963 | Italy |
|
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NA |
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BRIP 57892a |
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Australia |
|
NA | NA |
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BRIP 54736j |
|
Australia |
|
NA | NA |
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MFLUCC 16-0113 |
|
China |
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NA |
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CBS 129519 | Australia |
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CBS 144.27 | USA |
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CBS 113470 |
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Korea |
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CGMCC 3.17531 |
|
China |
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NA |
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CGMCC 3.17093 | China |
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NA |
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MFLU 18-0006 | Thailand |
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NA | NA | NA |
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BRIP 54847 |
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Australia |
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NA | NA |
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CBS 141329 |
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Malaysia |
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NA |
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NA |
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CGMCC 3.17532 |
|
China |
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NA |
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CBS 151.73 |
|
Netherlands |
|
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MFLUCC 16-0105 |
|
China |
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NA |
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CBS 101339 |
|
Dominican Republic |
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CBS 462.69 |
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Spain |
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CBS 338.89 |
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Yugoslavia |
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CBS 143770 |
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South Africa |
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CFCC 53103 |
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China |
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CBS 133181 |
|
Brazil |
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MFLU 15-1279 |
|
Italy |
|
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NA |
|
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BRIP 66528 | South Africa |
|
NA | NA | NA |
|
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CFCC 51634 |
|
China |
|
|
|
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MFLUCC 10-573a | Thailand |
|
NA | NA |
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FAU635 |
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USA |
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PSCG |
|
China |
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CBS 136969 |
|
Italy |
|
|
|
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|
ICMP20663 |
|
China |
|
NA |
|
|
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|
MFLU 17-1197 | on dead wood | China |
|
NA | NA |
|
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|
CBS 464.90 |
|
New Zealand |
|
|
|
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MFLUCC 16-0117 |
|
China |
|
NA | NA |
|
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MFLUCC 12-0777 |
|
Thailand |
|
|
NA |
|
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MFLUCC 13-0471 |
|
Thailand |
|
|
NA |
|
|
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MFLUCC 12-0767 |
|
Thailand |
|
|
NA |
|
|
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CBS 133180 |
|
Brazil |
|
|
|
|
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CFCC 51999 |
|
China |
|
|
|
|
|
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BRIP 62248a |
|
Australia |
|
NA | NA |
|
|
|
CFCC 52592 |
|
China |
|
|
|
|
NA |
|
CGMCC 3.17569 |
|
China |
|
NA |
|
|
|
CFCC 52594 |
|
China |
|
|
|
|
|
|
|
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|
China |
|
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NA |
|
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CGMCC 3.18282 |
|
China |
|
NA |
|
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CGMCC 3.18289 | China |
|
|
|
|
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CBS 121124 | China |
|
|
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|
|
Note: NA, not applicable. Strains in this study are marked in bold.
The five-gene sequence dataset (ITS,
Phylogram of
Continued
Distinguished from the phylogenetically closely-related species,
Named after the host species,
Asexual morph:
Culture incubated on
China. Hunan Province: Zhuzhou City, on leaves of
Three isolates representing
Asexual morph:
Culture incubated on
China. Hunan Province: Zhuzhou City, on leaves of
Distinguished from its phylogenetically closely-related species,
In reference to the Hunan province, from where the fungus was first collected.
Asexual morph:
Culture incubated on
China. Hunan Province: Zhuzhou City, on leaves of
Three isolates representing
Sexual morph:
Culture incubated on
China. Hunan Province: Zhuzhou City, on leaves of
In this study, an important oil-tea tree species,
The expanding cultivation of
According to the USDA Fungal–host interaction database, there are two records of
As the species concept of
This study is financed by the Research Foundation of Education Bureau of Hunan Province, China (Project No.: 19B608) and the introduction of talent research start-up fund project of