Corresponding authors: Hai-Xia Ma (
Academic editor: Thorsten Lumbsch
Three new species of
Pan X-Y, Song Z-K, Qu Z, Liu T-D, Ma H-X (2022) Three new
Species of
Hainan Province (
Voucher specimens are deposited in the Fungarium of the Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences (
A modified cetyltrimethylammonium bromide (
Species, specimens and GenBank accession number of sequences used in this study. New species and sequences are set in bold.
Taxon | Substrate / Origin | Specimen No. | GenBank No. | Reference | ||
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ITS | TUB | RPB2 | ||||
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termite nests / China Taiwan | HAST 623 |
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wood / Guadeloupe | HAST 570 |
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trunk / China Taiwan | HAST 94042903 |
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dead leaves / Guadeloupe | HAST 529 |
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bark / China Taiwan | HAST 90080804 |
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bark / China Taiwan | HAST 89041211 |
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wood / China Taiwan | HAST 93082814 |
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termite nests / China Taiwan | HAST 95052001 |
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bamboo culm / China Taiwan | HAST 95070101 |
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bamboo culm / Thailand | JDR 162 |
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bark / USA | JDR 256 |
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bark / China Taiwan | HAST 90112623 |
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leaves of |
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– | – |
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termite nests / China Taiwan | HAST 720 |
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wood / New Zealand | PDD 600 |
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termite nests / China Taiwan | HAST 664 |
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wood / French | HAST 786 |
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wood / China |
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– | – |
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bark / France | HAST 398 |
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log / Russian Far East | HAST 477 | – |
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pod / Thailand | JDR 189 |
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termite nests / China Taiwan | HAST 658 |
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bark / China Taiwan | HAST 92092013 |
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fallen leaves and petioles of |
HMJAU 22818 |
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– | – |
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herbaceous stem / Iran | GUM 1052 |
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– | – |
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termite nests / French West Indies | HAST 491 |
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fruit / French West Indies | HAST 431 |
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wood / China Taiwan | HAST 479 |
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termite nests / China Taiwan | HAST 641 |
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wood / Belgium | HAST 152 |
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wood / China Taiwan | HAST 95082001 |
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leaf debris / Sweden | CBS 122617 |
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– | – |
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fruit of |
HAST 553 |
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termite nests / China Taiwan | HAST 725 |
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HAST 92042501 |
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wood / Martinique | HAST 419 |
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bark / China Taiwan | HAST 95072910 |
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fruits of |
HAST 93090701 |
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wood / China |
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– | – |
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wood / Iran | IRAN 16582 F |
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– | – |
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petioles and infructescence of |
JDR 148 |
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wood / USA | JDR 259 |
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dead branch / French West | HAST 520 |
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termite nests / China Taiwan | HAST 653 |
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fallen seeds / USA | JDR 859 |
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fruits / Germany | LZ 2010-502 |
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– | – |
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fruits / New Zealand | PDD 604 |
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dead leaves / French West | HAST 528 |
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trunk / China Taiwan | HAST 91122401 |
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wood / USA | JDR 1012 |
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Stump / Germany | M:M-0125909 |
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– | – |
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log of |
HAST 920 |
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bark / China Taiwan | HAST 92092023 |
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fallen leaves / China Taiwan | HAST 90071613 |
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fallen leaves of |
JDR 258 |
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branch / China | HAST 304 |
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leaf litter or wood / Korea | KA12-0530 |
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– | – |
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leaf litter or wood / Korea | KA13-1324 |
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– | – |
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leaf litter or wood / Korea | KA13-1325 |
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– | – |
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twigs / USA | HAST 94080508 |
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bark / China Taiwan | HAST 88113002 |
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wood / Iran | GUM 1151 |
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– | – |
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bark / France | HAST 383 |
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– |
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– / Puerto Rico | ATCC 28093 |
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– |
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The molecular phylogeny was inferred from a combined dataset of ITS, TUB and RPB2 sequences. The sequences retrieved from open databases originated from
A combined matrix of ITS-RPB2-TUB and ITS-exons of TUB and RPB2 were used to construct phylogenetic analysis by two methods including maximum likelihood (
This study used genetic sequences of 57 species, including 69 ITS sequences, 57 TUB sequences and 54 RPB2 sequences. We applied two tree construction methods to improve the reliability of the results.
After the alignment sequence was adjusted using MAFFT, the ITS alignment, shown in BioEdit 7.0.5, consisted of 778 character positions, 2219 in the TUB alignment and 1241 in the RPB2 alignment. After curing, the constructed multigene alignment (
The analysis results show that the phylogenetic tree, generated by
Phylogenetic tree of
Differs from
China. Hainan Province, Lingshui County, Diaoluoshan Natural Reserve, on fallen leaves of
“
China. Hainan Province, Lingshui County, Diaoluoshan Natural Reserve, on fallen leaves of
Differs from
China. Hainan Province, Lingshui County, Diaoluoshan Natural Reserve, on fallen leaves of
“
China. Hainan Province, Lingshui County, Diaoluoshan Natural Reserve, on fallen leaves of
Differs from
China. Hainan Province, Lingshui County, Diaoluoshan Natural Reserve, on fallen leaves of
“
China. Hainan Province, Lingshui County, Diaoluoshan Natural Reserve, on fallen leaves of
We included ten
Until now, ten taxa,
1 | Stromata with rounded fertile apices |
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– | Stromata with acute sterile apices |
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2 | Stromata associated with leaves and petioles of |
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– | Stromata associated with leaves of |
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3 | Stipes tomentose |
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– | Stipes glabrous |
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4 | Fertile part subglobose |
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– | Fertile part not subglobose |
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5 | Stromata cylindrical |
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– | Stromata filiform |
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6 | Ascospores (5.5–)6–8 × 3–3.5(–4) µm |
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– | Ascospores length > 8.5 µm |
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7 | Stromata with conspicuous perithecial contours, ascospores (11.5–)12.5–14.5(–15) × 5.5–8 µm |
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– | Stromata with inconspicuous perithecial contours, ascospores (8.5–)9–11 × 4–6 µm |
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8 | Ascospores 16.5–20(–21.5) × 4–5(–6) µm |
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– | Ascospores length < 16.5 µm |
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9 | Stromata associated with leaves of |
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– | Stromata associated with leaves of |
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The authors thank Prof. Yu-Ming Ju (Institute of Plant and Microbial Biology, Academia Sinica, Taiwan, China) for suggestions on the manuscript. This study was supported by the National Natural Science Foundation of China (no. 31770023, 31972848, U1803232). We are also grateful to the Key Research and Development Program of Hainan (ZDYF2020062) and Hainan Basic and Applied Research Project for Cultivating High-Level Talents (2019RC305).