Corresponding author: Zefen Yu (
Academic editor: R. Phookamsak
Members of
Yang XQ, Ma SY, Peng ZX, Wang ZQ, Qiao M, Yu Z (2021) Diversity of
The genus
Species of
During our investigation of endophytic fungal diversity of aquatic plants in southwest China, among 1697 acquired strains, 112 strains belonging to
Samples were collected from Yunnan, Guizhou, Sichuan provinces, Chongqing and Tibet from 2014 to 2017. The dominant hosts are
Pure cultures were deposited in the
Actively growing mycelium was scraped off from the surface of the culture and transferred to 2 ml Eppendorf micro-centrifuge tubes. Total genomic DNA was extracted follow the protocol of
Species | Strain no. | GenBank accession no. | |||
---|---|---|---|---|---|
LSU |
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||
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CBS 379.77 |
|
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– | – |
|
CBS 113362T |
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CBS 131740 |
|
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|
CBS 131741T |
|
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|
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|
3F17 |
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3F24 |
|
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|
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3F42 |
|
|
|
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|
3F43 |
|
|
|
|
|
CBS 131739 |
|
|
– | – | |
CBS 137.37 |
|
|
– | – | |
|
|
|
|
|
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|
CBS 116708T |
|
|
|
|
|
|
|
|
|
|
|
CGMCC 3.19658T |
|
|
– | – |
CGMCC 3.19659 |
|
|
– | – | |
CGMCC 3.19660 |
|
|
– | – | |
|
CBS 144925 |
|
|
– | – |
|
CBS 423.66T |
|
|
– | – |
|
KUMCC 18-0181 |
|
|
– | – |
|
CBS 489.96T |
|
|
|
|
CBS 409.95 |
|
|
– |
|
|
|
CGMCC 3.19656T |
|
|
– | – |
CGMCC 3.19657 |
|
|
– | – | |
|
CGMCC 3.15078T |
|
|
|
|
CGMCC 3.15079 |
|
|
|
|
|
|
NJM 0662T | – |
|
– | – |
NJM 0665 | – |
|
– | – | |
|
CBS 131744 |
|
|
|
– |
CBS 131745T |
|
|
|
– | |
|
|
|
|
– | |
|
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|
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|
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CBS139623T |
|
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|
CBS 139624 |
|
|
– | – | |
|
CBS131742T |
|
|
|
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|
CBS 131743 |
|
|
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|
CBS 131861T |
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|
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|
|
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|
ACCC 39144 |
|
|
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|
ACCC39145T |
|
|
|
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|
ACCC 39146 |
|
|
|
|
|
|
CBS 144924T |
|
|
– | – |
JW 13006 |
|
|
– | – | |
JW 62006 |
|
|
– | – |
Strains and sequences generated in this study are emphasized in bold face. Tex-type cultures.
The
Maximum-likelihood (
A total of 112 strains were identified as members of
Phylogenetic tree inferred from a Bayesian analysis based on
The four Bayesian trees derived from the single-gene sequence alignments (LSU,
Phylogenetic tree of
Latin,
Colony on
Colonies on
China, Yunnan Province, Kunming, The Dian Lake,
Although the phylogenetic analyses showed that our isolate
Latin,
Colony on
Colonies on
China, Sichuan Province, Daofu,
In the phylogenetic tree, the closest species to
Colony on
Colonies on
China, Sichuan Province, Baiyu,
Colony on
Colonies on
China, Yunnan Province, Erhai,
Sexual reproduction of
In this study, among 129 sample sites in Yunnan and Sichuan province,
Secondly, the separation frequency of
This study revealed
This work was financed by the National Natural Science Foundation Program of P.R. China (31770026, 31760012). We are grateful to two reviewers for critically reviewing the manuscript and for providing helpful suggestions to improve this paper.
Figures S1–S4
phylogenetic tree