Corresponding author: Yan-Feng Han (
Academic editor: T. Lumbsch
Chen W-H, Han Y-F, Liang J-D, Tian W-Y, Liang Z-Q (2020) Morphological and phylogenetic characterisations reveal three new species of
The genus
Three infected insect specimens were collected during a survey of entomogenous fungi in south-western China. Morphological and molecular phylogenetic analyses suggested that these isolates represented three new species, which are described here as
Three fungus-infected insect specimens were collected from Xishui County (
Macroscopic and microscopic morphological characteristics of the fungi were examined and growth rates determined from
DNA extraction was carried out in accordance with
The DNA sequences, generated in this study, were assembled and edited using Lasergene software (version 6.0 DNASTAR). Generated
Taxa included in the phylogenetic analyses.
Species | Strain No. | GenBank Accession No. | |||
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RPB1 |
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HUA 772 |
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CBS 402.78 |
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NHJ 6709 |
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CBS 541.81 |
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TBRC 7242 |
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TBRC 7243 |
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TBRC 7244 |
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CBS 101247 |
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TBRC 7247 |
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TBRC 7248 |
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TBRC 7249 |
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TBRC 7245 |
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TBRC 7246 |
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BCC 16819 |
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OSC111002 |
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TBRC 7250 |
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TBRC 7251 |
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TBRC 7252 |
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P.C. 546 |
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HUA 179219 |
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QCNE 186726 |
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ARSEF 1564 |
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ARSEF 617 |
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BCC 16585 |
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ARSEF 2567 |
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MCA 1557 |
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QCNE 186272 |
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QCNE 186714 |
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HUA 179217 |
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HUA 179218 |
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ARSEF 7760 |
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ARSEF 3405 |
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ARSEF 5689 |
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ARSEF 5718 |
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OSC 93609 |
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OSC 93610 |
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NBRC 101409 |
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NBRC 101410 |
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CBS 729.73 |
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CBS 107.73 |
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EFCC 5690 |
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EFCC 8260 |
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TBRC 7253 |
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TBRC 7254 |
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TBRC 7255 |
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TBRC 7256 |
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TBRC 7257 |
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CBS 152.83 |
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TBRC 7258 |
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CBS 153.83 |
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OSC 111004 |
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ARSEF 5691 |
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NHJ 12623 |
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TBRC 7274 |
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CBS 110.73 |
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CBS 111113 |
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CBS 107.10 |
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CBS 244.31 |
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CBS 375.70 |
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CBS 337.52 |
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CBS 134.22 |
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TBRC 7259 |
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TBRC 7260 |
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TBRC 7261 |
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TBRC 7262 |
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ARSEF 7218 |
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ARSEF 8058 |
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EFCC 5886 |
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TBRC 7263 |
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TBRC 7264 |
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OSC 93623 | ||||
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TBRC 7275 |
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TBRC 7276 |
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EFCC 5197 |
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EFCC 5693 |
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EGS 38.165 |
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EGS 38.166 |
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NHJ 10627 |
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NHJ 10684 |
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ARSEF 4358 |
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CBS 116719 |
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ARSEF 5413 |
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CBS 102184 |
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BCC 28612 |
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ARSEF 5135 |
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OSC 111007 |
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TBRC 7265 |
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TBRC 7266 |
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TBRC 7267 |
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CBS 309.85 |
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NHJ 12014 |
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ARSEF 1915 |
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NHJ 10788 |
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NHJ 13158 |
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NHJ 10808 |
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NHJ 5401 |
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NHJ 7859 |
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NHJ 3510 |
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BCC 41864 |
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NHJ 4314 |
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NHJ 10469 |
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NHJ 11923 |
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NHJ 13117 |
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NHJ 13161 |
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P.C. 602 |
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CBS 350.85 |
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CBS 726.73a |
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CBS 164.70 |
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CBS 101270 |
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CBS 363.86 |
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CBS 532.81 |
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CBS 284.36 |
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CBS 431.87 |
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CBS 240.32 |
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CBS 262.58 |
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TBRC 7271 |
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TBRC 7272 |
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TBRC 7273 |
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TBRC 7915 |
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TBRC 7916 |
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TBRC 7270 |
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CBS 116.25 |
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CBS 101267 |
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CBS 704.86 |
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CBS 101237 |
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Maximum Likelihood (
The phylogenetic tree of
Phylogenetic relationships of the genus
Analysis 1:
Analysis 2:
Phylogenetic relationships between the genus
Differs from
China, Guizhou Province, Xishui County (
Colonies on
Snout beetle, family
Xishui County, Guizhou Province, China.
Referring to its insect host, order
Morphological comparison of three new species with other
Species | Morphological characteristics | Reference | ||
---|---|---|---|---|
Phialide (μm) | Conidia (μm) | Hosts/substrates | ||
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5–8 × 2 | ovate to lemon-shaped | soil, lepidopterous pupa |
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2.3–2.5(–3) × 1.5–1.8 | ||||
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cylindrical to ellipsoidal | fusiform | lepidopterous larvae |
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(5–)5.5–8.5(–13) × 2–3 | (2–)2.5–3.5(–4) × (1–)1.5(–2) | |||
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cylindrical | short fusiform | lepidopterous larvae |
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(4–)6.5–10(–12) × (1–)1.5–2(–3) | (2–)3(–3.5) × 1.5–2 | |||
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cylindrical to ellipsoidal | fusiform, ellipsoidal or subglobose | snout beetle | this study |
5.4–9.7 × 1.2–1.8 | 1.7–2.5 × 1.2–1.8 | |||
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cylindrical | fusiform to ovoid | bee | this study |
6.5–10.6 × 1.2–2.0 | 1.9–2.5 × 1.5–2.1 | |||
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ellipsoidal | fusiform to subglobose | lepidopterous pupa | this study |
5.2–8.5(–13.1) × 1.1–1.7 | 2.0–2.5 × 1.2–2.0 |
Differs from
China, Guizhou Province, Xishui County, at
Colonies on
Bee, family
Xishui County, Guizhou Province, China.
Referring to its insect host, order
Differs from
China, Guizhou Province, Rongjiang County (
Colonies on
Pupa, order
Rongjiang County, Guizhou Province, China
Referring to its insect host, order
Phylogenetic analyses, based on the combined datasets of (
The evolutionary dynamics of fungi and their hosts are usually described either by co-evolution or by host shifts. Shifts often occur to new hosts that are evolutionarily distant, but which occupy a common ecological niche (
In the present study, a four loci phylogenetic analysis showed that
This work was supported by the National Natural Science Foundation of China (Grant No. 31860002), High-level Innovative Talents Training Object in Guizhou Province (No. Qiankehepingtairencai [2020]6005), Science and Technology Foundation of Guizhou Province (No. Qiankehejichu [2020]1Y060), National Survey of Traditional Chinese Medicine Resources (No. Caishe [2017]66, 216) and Engineering Research Center of General Higher Education in Guizhou Province (Qianjiaohe (2015) 337). We also thank Lesley Benyon, PhD, from Liwen Bianji, Edanz Group China (