Corresponding authors: Fang Wu (
Academic editor: Alfredo Vizzini
In this study, taxonomic and phylogenetic analyses of
Du P, Wu F, Tian X-M (2020) Three new species of
Corda established the genus
During recent studies on wood-inhabiting fungi in China, samples morphologically belonging to
The samples were evaluated and submitted at the Institute of Microbiology herbaria of
Genomic DNA was isolated from the dried specimens using the CTAB rapid plant genome extraction kit from Aidlab Biotechnologies (Beijing, China), as per provided guidelines with few alterations. The ITS5 and ITS4 primers were used (
Phylogenetic analyses were applied to the combined ITS+nLSU dataset. Sequences generated in this study were aligned with additional sequences downloaded from GenBank (Table
Information for the sequences used in this study.
Species | Specimen no. | Locality | GenBank accession no. | |
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ITS | nLSU | |||
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HHB 4100-Sp | United States |
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KH Larsson 11977 | Sweden |
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LE 247382 | Russia |
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Miettinen 2312 | Finland |
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Miettinen X1080 | Sweden |
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Miettinen 7429 | Finland |
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Labrecque & Labbé 372 | Canada |
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MUAF 888 | Czech Republic |
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Niemelä 2752 | Canada |
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MW 331 | Canada |
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BRNM 710170 | Czech Republic |
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Cui10525 | China |
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Yuan 9654 | China |
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Dai 15253 | China |
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Dai 12045 | China |
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Niemelä 7691 | Finland |
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Dai 17540 | China |
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Dai 17679 | China |
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Spirin 2957 | Russia |
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KH Larsson 11848 | Sweden |
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Miettinen 13852 | Indonesia |
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Miettinen 2954 | Indonesia |
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Dai 19138 | China |
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Miettinen 2091 | Russia |
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Nuñez 1065 | Japan |
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Niemelä 8246 | Finland |
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KH Larsson 13238b | Estonia |
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Spirin 2652 | Russia |
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Dai 21107 | China |
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Dai 21108 | China |
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KH Larsson 11903 | Sweden |
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M Kulju 1004 | Finland |
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Miettinen X460 | Australia |
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Miettinen 10026 | China |
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Dai 19344 | China |
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Dai 19345 | China |
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K Hjortstam 18286 | Sweden |
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B Seitzman 2008-100 | USA |
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Miettinen 11038 | Finland |
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Dai 15709 | China |
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L 13763sp | Canada |
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Bureid 110510 | Norway |
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Saarenoksa 10195 | Finland |
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Ryvarden 47033 | Estonia |
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KH Larsson 11905 | Sweden |
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Spirin 2189 | Russia |
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Spirin 2367 | Russia |
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KH Larsson 11902 | Sweden |
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GB 1195 | Sweden |
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KH Larsson 13849 | France |
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KH Larsson 12316 | United States |
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Miettinen 8065 | Finland |
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Spirin 2116 | Russia |
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Meijer et al. 3637 | Brazil |
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|
New sequences are shown in bold.
The dataset included 54 fungal collections representing 48 species. The best model for the dataset estimated and applied in the
The phylogeny of three new species illustrated by Bayesian Inference tree and other taxa according to the combined ITS+nLSU dataset. Labelling of branches is done with
Refers to the species being collected in the south of China.
Annual, resupinate, soft corky, without odour or taste when fresh, corky when dried, 7 cm length, 4 cm width and 0.4 mm thick at centre. Pore surface white when fresh, cream to buff-yellow when dried; margin distinct, white and nearly 1 mm width; pores round to angular, 9–11 per mm; dissepiments thin, entire. Subiculum cream, paler than tubes, corky when dried, nearly 0.1 mm thick. Tubes concolorous with pore surface, corky, nearly 0.3 mm length.
Basidiomata of
Hyphal system dimitic; generative hyphae with clamp connections, skeletal hyphae
Dominated by skeletal hyphae; generative hyphae hyaline, thin- to fairly thick walled, rarely branched, 2–3.5 µm in diam.; skeletal hyphae thick-walled with a wide to narrow lumen, flexuous, unbranched, gelatinised, interwoven, 3–4 µm in diam.
Trama dominated by skeletal hyphae; generative hyphae hyaline, thin- to fairly thick walled, rarely branched, 2–3 µm in diam.; skeletal hyphae thick-walled with a wide to narrow lumen, unbranched, more or less straight, subparallel amongst the tube, 2.5–3.8 µm in diam. Skeletocystidia clavate, thick-walled, originated from trama, apex covered with crystals, embedded amongst trama and dissepiments or projecting into hymenium, 30–40 × 6–8 µm; smaller skeletocystidia clavate, thick-walled, 14–18 × 5–6 µm. Basidia barrel-shaped, bearing four sterigmata and a basal clamp connection, 7–8 × 4–4.5 µm; basidioles in shape similar to basidia, but smaller.
Basidiospores smooth, ellipsoid, thin-walled, hyaline,
China, Yunnan Province, Jinghong, Virgin Forest Park, on fallen bamboo, 17.VI.2017 Dai 17540 (holotype, BJFC025072, isotype in
Microscopic assessment of
Refers to the species growing on
Annual, resupinate, coriaceous, without odour or taste when fresh, hard corky when dried, 30 cm length, 3 cm width and 1 mm thick. Pore surface flesh-pink when fresh, pink to salmon when dried; margin distinct, white and nearly 3 mm width; pores round to angular, 6–8 per mm; dissepiments thin, entire. Subiculum buff, paler than tubes, corky when dried, nearly 0.5 mm thick. Tubes concolorous with pore surface, corky, nearly 0.5 mm length.
Basidiomata of
Hyphal system dimitic; generative hyphae with clamp connections, skeletal hyphae
Dominated by skeletal hyphae; generative hyphae hyaline, thin-walled, unbranched, 2–3 µm in diam.; skeletal hyphae thick-walled to subsolid, flexuous, unbranched, gelatinised, interwoven, 2.5–4 µm in diam.
Trama dominated by skeletal hyphae; generative hyphae hyaline, thin-walled, rarely branched, 2–3 µm in diam.; skeletal hyphae thick-walled to subsolid, unbranched, flexuous, more or less gelatinised, interwoven, 2.5–3.5 µm in diam. Skeletocystidia clavate, thick-walled, originated from trama, apex covered with crystals, embedded amongst trama and dissepiments or projecting into hymenium, 22–45 × 6–8 µm. Basidia clavate, bearing four sterigmata and a basal clamp connection, 8–11 × 4–4.6 µm; basidioles in shape similar to basidia, but smaller.
Basidiospores ellipsoid, hyaline, thin-walled, smooth,
China, Chongqing, Nanchuan County, Jinfoshan Forest Park, on dead tree of
Microscopic assessment of
Refers to the species similar to
Annual, resupinate, coriaceous, without odour or taste when fresh, hard corky when dried, 8 cm length, 3 cm width and 1.5 mm thick. Pore surface pale salmon when fresh, brownish-vinaceous when dried; margin indistinct to almost lacking; pores round to angular, 10–12 per mm; dissepiments thin to fairly thick, entire. Subiculum vinaceous, darker than pores, hard corky when dried, nearly 0.3 mm thick. Tubes vinaceous, distinctly darker than pore surface, rigid, nearly 1.2 mm length.
Basidiomata of
Hyphal system dimitic; generative hyphae with clamp connections and simple septa, skeletal hyphae
Dominated by skeletal hyphae; generative hyphae hyaline, thin- to fairly thick-walled, frequently branched, 2.5–3 µm in diam.; skeletal hyphae thick-walled with a wide to narrow lumen, flexuous, occasionally branched, more or less gelatinised, interwoven, 2–4 µm in diam.
Trama dominated by skeletal hyphae; generative hyphae hyaline, thin- to fairly thick-walled, frequently branched, with both simple septa and clamp connections, simple septa especially common at dissepiment edge, 2–3.2 µm in diam.; skeletal hyphae thick-walled with a wide to narrow lumen, rarely branched, flexuous, more or less gelatinised, interwoven, 2.5–3.5 µm in diam. Skeletocystidia clavate, thick-walled, originated from trama, apex covered with crystals, embedded amongst trama and dissepiments or projecting into hymenium, 35–50 × 6–9 µm. Fusoid cystidioles present, 8–14 × 3.5–2.5 µm; basidia clavate, bearing four sterigmata and a basal clamp connection, 10–12 × 4–5 µm; basidioles in shape similar to basidia, but smaller.
Basidiospores mostly lunate, hyaline, thin-walled, smooth, sometimes with one or two small guttules,
China, Yunnan Province, Yongping County, Baitaishan Forest Park, on rotten angiosperm wood, 7.XI.2018 Dai 19345 (holotype, BJFC027813, isotype in
Microscopic structures of
Phylogenetically,
Three new species of
The research was supported by the National Natural Science Foundation of China (Project Nos. 31970014 and 31900019), Chongqing Education Commission Project (KJQN201901427) and Shandong Provincial Universities Outstanding Youth Innovation and Technology Program (2019KJE003). We express our gratitude to Prof. Yu-Cheng Dai (