Corresponding author: Tolgor Bau (
Academic editor: M. P. Martín
Species of the genus
Liu H-Y, Li Y-C, Bau T (2020) New species of
The genus
During field investigation of
Specimens were described and photographed in the field and deposited in the
Protocols for DNA extraction, PCR, sequencing and sequence alignment followed those in
19 sequences (10 of
Specimens used in molecular phylogenetic study and their GenBank accession numbers.
Species | Voucher | Locality | Accession | Reference | |
---|---|---|---|---|---|
|
|
||||
|
Li1215 |
|
|
|
|
|
Li1224 |
|
|
|
|
|
LC_LJW237 |
|
|
|
|
|
Li993 |
|
|
|
|
|
RH7247 | Costa Rica |
|
– |
|
|
RH7189 | Costa Rica |
|
– |
|
|
Wu445 |
|
|
|
|
|
Cui47 |
|
|
|
|
|
BD210 | USA |
|
– |
|
|
snBoth | USA |
|
|
|
|
Halling10162 | USA |
|
|
|
|
Wu317 |
|
|
|
|
|
Tyni1 | Japan |
|
– |
|
|
FHMU2045 | Southern China |
|
|
|
|
FHMU2800 | Southern China |
|
|
|
|
201/97 | USA |
|
– |
|
|
Halling10163 | USA |
|
|
|
|
161/97 | USA |
|
– |
|
|
Zeng647 | Southern China |
|
|
|
|
FHMU375 | Southern China |
|
|
|
|
Zeng668 |
|
– |
|
|
|
96/97 | USA |
|
– |
|
|
22/97 | USA |
|
– |
|
|
116/96 | USA |
|
– |
|
|
57/97 | USA |
|
– |
|
|
Zeng1299 |
|
|
|
|
|
Zeng1278 |
|
|
|
|
|
Zeng569 | Southern China |
|
– |
|
|
LJ258 |
|
|
|
|
|
LJ260 |
|
|
|
|
CAL_F_1397 | India |
|
– | GenBank | |
|
HY56 |
|
– |
|
|
|
CFMR:DR-1035 | Dominican Republic |
|
– |
|
|
CFMR:BZ-2386 | Belize |
|
– |
|
|
Li1951 |
|
|
|
|
|
Li1073 |
|
|
|
|
|
HMJAU4637 |
|
|
|
|
|
Liang521 |
|
|
|
|
|
LF690 | Southern China |
|
|
Li et al. 2014 |
|
Li915 |
|
|
Li et al. 2014 |
Sequences obtained in this study are shown in bold.
The combined nuclear dataset (
Maximum likelihood phylogenetic tree of
China. Yunnan Province: Jingdong County, Ailaoshan National Nature Reserve, alt. 2500 m, 14 July 2008, Y.C. Li 1215 (holotype:
Habitat of the new
Basidiomata small to medium-sized. Pileus 3–5 cm in diameter, hemispherical to applanate, surface dry, densely subtomentose, black (4F3) to blackish (4E2) in the center and gray (3D1) or yellowish-gray (3C2-3) towards margin, context 2.5 cm thick in the center of the pileus, pallid gray (2D1) to cream (2C3-4), unchanging when bruised. Hymenophore adnate or slightly depressed around apex of stipe; pores angular, tubes up to 11 mm long, 0.3–1 mm wide, white (2B1) when young and yellowish (2A2) in age, becoming brownish-yellow (5C7-8) when injured. Stipe 4–6 × 0.8–1.2 cm, clavate to flexuous, solid; surface dry, blackish to gray, prominently and coarsely reticulate over the upper 1/3; context white (2A1) in the upper part and yellowish to cream yellow downwards, unchanging when injured; basal mycelium white (2A1). Taste and odor indistinct.
Basidia 26–38 × 6–10 μm, clavate, thin-walled, 4-spored, hyaline to yellowish in KOH. Basidiospores [60/3/2] (7)8–10.5(11) × 3–4.5(5) μm [Q = (1.89) 2–3.33 (3.67), Qm = 2.52 ± 0.42], subfusiform and inequilateral in side view with shallow suprahilar depression, elongate fusoid or narrowly oblong in ventral view, slightly thick-walled (up to 0.5 μm), brownish to yellowish-brown in KOH, olive-brown to brown in Melzer’s reagent, smooth. Hymenophoral trama boletoid; hyphae cylindrical, 3.5–9 μm wide, hyaline to yellowish in KOH, yellowish to brownish-yellow in Melzer’s reagent. Cheilo- and pleurocystidia 26–55 × 6–10 μm, abundant, subfusiform to fusiform, thin-walled, with yellowish-brown contents, surface without encrustations. Caulocystidia forming the reticulum over the stipe surface, similar to cheilo- and pleurocystidia. Pileipellis a trichoderm about 280 μm thick, composed of more or less vertically arranged, slightly interwoven, brown to dark brown hyphae, 5–15 μm wide; terminal cells 45–111 × 9–15 μm, narrowly clavate to subcylindrical or subfusiform, sometimes narrowly mucronate, rostrate, slightly thick-walled (up to 0.5 μm), hyaline to yellowish in KOH, yellowish to brownish-yellow in Melzer’s reagent. Pileal trama composed of thin- to slightly thick-walled (up to 0.5 μm) hyphae, 5–11 μm wide, hyaline to yellowish in KOH, yellowish to brownish-yellow in Melzer’s reagent. Clamp connections absent in all tissues.
Microscopic features of
Solitary on the ground in forests dominated by plants in the family
China. Yunnan Province: Jingdong County, Ailaoshan National Nature Reserve, alt. 2500 m, 14 July 2008, Y.C. Li 1224 (
In the phylogenetic analysis (Fig.
China. Liaoning Province: Anshan City, Qianshan, alt. 400 m, 25 Aug 2015, J. Li 260 (holotype:
Microscopic features of
Basidiomata medium-sized. Pileus 6–7.2 cm in diameter, subhemispherical to applanate, sometimes convex; surface tomentose, grayish-brown (5D2-3) to brown (5E4), rimose when dry, always cracked into small squamules on grayish (4B1) to whitish (2A1) background; context 1–2 cm thick in the center of pileus, white (1A1), unchanging when injured. Hymenophore adnate, sometimes slightly depressed around apex of stipe; pores angular, 0.3–1 mm wide, tubes up to 14 mm long, yellow (4C3-4) to grayish-yellow (4B2-3), unchanging when injured. Stipe 6–8 × 1.1–1.5 cm, subcylindrical, solid; surface dry, pale yellow at apex, blackish-yellow towards the base, entirely covered with moderately developed reticulum; context white to cream in the upper part and yellowish to yellow downwards, unchanging when injured; basal mycelium yellow. Taste and odor indistinct.
Basidia 21–27 × 9–11 μm, clavate, thin-walled, four-spored; sterigmata 4–5 μm long. Basidiospores [40/2/2] (9) 10.0–13.5 (–14.0) × (3) 4.0–5.0 (–5.5) μm, Q = (2.25–) 2.5–3.25 (–3.42), Qm = 2.88 ± 0.32, subfusiform to ellipsoid, slightly thick-walled (up to 0.5 μm), hyaline to yellowish in KOH, olive-brown to yellowish-brown in Melzer’s reagent, smooth. Hymenophoral trama boletoid. Cheilo- and pleurocystidia 35–56 × 7–12 μm, abundant, subfusiform to fusiform, thin-walled, with yellowish-brown to brown contents, without encrustations. Pileipellis a subcutis, 100–120 μm thick, composed of thin-walled filamentous hyphae 4–7 μm wide, with subcylindrical to clavate terminal cells 33–72 × 4–6 μm, sometimes with subacute apex, colorless to pale yellowish-brown in KOH, yellow-brown to brownish in Melzer’s reagent. Pileal trama composed of thin-walled hyphae 4–9 μm wide, colorless to pale yellowish-brown in KOH, yellow-brown to brownish in Melzer’s reagent. Clamp connections absent in all tissues.
Solitary on the ground in mixed forests dominated by plants in the families
China. Liaoning Province: Anshan City, Qianshan, alt. 400 m, 25 Aug 2015, J. Li 258 (
Nine species of
1 | Hymenophore bright yellow to brownish-yellow, stipe yellow to orange-yellow, mycelium on the base of stipe yellow to brownish-yellow |
|
– | Hymenophore whitish to grayish white, stipe black to blackish or grayish-black, mycelium on the base of stipe whitish to grayish-white |
|
2 | Pileus grayish-brown to brown without olivaceous tinge, context in pileus white to grayish-white |
|
– | Pileus yellow-brown to olive-brown, context in pileus yellow to pale yellow |
|
3 | Basidiomata medium-sized to large, pileus up to 15 cm in diameter, basidiospores 9–13 × 4–5 μm, cheilo- and pleurocystidia 30–60 × 6–10 μm |
|
– | Basidiomata small to medium-sized, pileus up to 8 cm in diameter, basidiospores 8–11 × 3.5–4 μm, cheilo- and pleurocystidia 20–46 × 4.5–7 μm |
|
4 | Context in the stipe white to grayish-white with olivaceous tinge |
|
– | Context in the stipe white to grayish-white with grayish-yellow tinge |
|
5 | Hymenophore white when young, lilac to purplish when old, basidiospores 9–11 × 4–5 μm |
|
– | Hymenophore white to grayish-white without lilac to purplish tinge, basidiospores relatively small 8–10.5 × 3.5–4 μm |
|
6 | Stipe entirely reticulate |
|
– | Stipe without reticulum or with reticulum restricted to the upper part |
|
7 | Pileus brown to blackish-brown, basidiospores 9.5–11 × 4–4.5 μm, pileipellis hyphae up to 8 μm wid |
|
– | Pileus grayish-brown to grayish-black, basidiospores slightly narrower 9–12 × 3.5–4 μm, pileipellis hyphae broad up to 13 μm wide |
|
8 | Pileus pale brown to grayish-brown, stipe without reticulum, basidiospores 10–12.5 × 4.5–5 μm, pleurocystidia 50–80 × 9–14.5 μm |
|
– | Pileus black to blackish, stipe covered with reticulum over the upper 1/3, basidiospores much smaller 8–10.5 × 3–4.5 μm, pleurocystidia relatively small 26–55 × 6–10 μm |
|
The authors are indebted to Dr. Jing Li (Kunming Institute of Botany, Chinese Academy of Sciences) for her kind help in collecting the specimens. They are grateful to Prof. Roy E. Halling (the New York Botanical Garden) for providing American specimens. The anonymous reviewers are acknowledged for their valuable comments and suggestions. This study was supported by the Funds of the National Natural Science Foundation of China (31570025, 31750001, 31872618), the Youth Innovation Promotion Association, CAS (2016348), the Key Research Program of Frontier Sciences of CAS (QYZDY-SSW-SMC029-5) and the Ten Thousand Talents Program of Yunnan (YNWR-QNBJ-2018-125).