Corresponding author: Boris Armel Olou (
Academic editor: M.-A. Neves
Autors (2020) Diversity of
The genus
Since the first formal description of the genus
Previous studies on
For Benin, seven species of
Additional to these known species in Benin, we recently found a putatively new species of
Our study thus aims to report the diversity of
A total of 37 specimens of
At least one sequence per specimen was generated for each morphotype except for the morphotype named
Taxa names with collection details and GenBank accession numbers of all sequences of
Species name | Voucher or strain | Origin | GenBank N° | Reference | ||||
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FP11801 |
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FP105043sp | USA: Mississippi |
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HHB4626sp | USA |
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FP105679sp | USA/Georgia |
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HHB6551 | USA/Florida |
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FP105038sp | USA: Mississippi |
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HHB9942sp | USA |
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Dai6847 |
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unpublished | |||||
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MUCL:40167 | Malawi |
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Dollinger 629 | USA/Florida |
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unpublished | |||||
DMC814 | Cameroon |
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unpublished | ||||
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Dai 14386 | China |
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unpublished | ||
Cui-7096 |
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unpublished | |||||
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FP106793sp | USA/Mississippi |
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TJV93_213sp | USA/Mississippi |
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AJ177 | USA: Florida |
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UZ526_17 | Malaysia |
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unpublished | |||||
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FP103976sp | USA: FLorida |
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FP106037T | USA |
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PR1133 | Puerto Rico |
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FPRI10 | Philippines |
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FP150762 | Belize |
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DMC811 | Cameroon |
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unpublished | ||||
CBS 158.35 |
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DMC815 | Cameroon |
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unpublished | |||
L11664sp | England |
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DMC341 | Cameroon |
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unpublished | |||
RLG5133T | USA: New York |
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145295(O) |
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unpublished | ||||
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DMC346 | Cameroon |
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unpublished | |||
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CBS 109427 | Taiwan |
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LIP:GUY09-110 | French Guiana |
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Dai6865 |
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unpublished | |||||
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BCC 33266 | Thailand |
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unpublished | ||||
Yuan5493 |
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Wei1653 |
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unpublished | ||||
Li286 |
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OH189sp | Venezuela |
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PRSC82 | Puerto Rico |
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BRFM<FRA>:1368 | Martinique |
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Dai6782 |
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unpublished | |||||
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Philippines |
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CBS:453.76 | India |
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HHB13445sp | USA/Michigan |
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Dai2005 | China |
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unpublished | ||||
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DMC360 | Cameroon |
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unpublished | |||
DMC817 | Cameroon |
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DMC816 | Cameroon |
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FP103050sp | USA/Florida |
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DMC370 | Cameroon |
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unpublished | |||
Cui 11040 | China |
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BKW004 | Ghana |
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FP101414sp | USA/Wisconsin |
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BCC26408 | Thailand |
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unpublished | ||||
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BCC27595 |
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unpublished | ||||
FRI437T |
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FPRI390 | Philippines |
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OH271sp | Venezuela |
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M0138339 | Papua New Guinea |
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PRSC95 | Puerto Rico |
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BCC 36861 | Thailand |
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unpublished | ||||
8R_1_2 | Thailand |
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CBS:614.73 | Sri Lanka |
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BJFC12724 | China |
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unpublished | |||
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LIP:GUY08-156 | French Guiana |
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BC1 | Finland |
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LIP:GUY08-167 | French Guiana |
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FP102529sp | USA/Wisconsin |
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Dai 10729 | China |
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unpublished | ||||
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FP135156sp | USA/New York |
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FP71974R | USA/Tennessee |
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The rows referring to sequences generated in this study are written in bold.
Macromorphology of
Macro-morphological descriptions were based on fresh and dried herbarium specimens. Microstructures are described using dried herbarium specimens. Fine sections through the basidiomata were prepared for observation using a razor blade under a stereomicroscope Leica EZ4 and mounted in 5% aqueous solution of potassium hydroxide (KOH) mixed with 1% aqueous solution of Phloxine. Melzer’s reagent (to test for dextrinoid or amyloid reactions), Cotton Blue (to test for cyanophilic reaction) were used and then examined at a magnification of 1000× using a Leica DM500 light microscope. Measurements were done with the software “Makroaufmaßprogramm” from Jens Rüdigs (
All alignments and phylogenetic trees generated in this study are available in TreeBASE under this link:
The phylogenetic trees generated from individual gene regions
Crossection of the hymenium at the base of a pore of
BENIN. Atlantic province, dry dense forest of Pahou in Ouidah,
Basidiomata probably perennial, sessile, pileate, applanate, semicircular, up to 13 cm long and 8 cm wide, up to 2.5 cm thick at the base, coriaceous to woody and hard when dry, without odour or taste when fresh. Pileus surface dull, glabrous, and whitish, zonate, margin thick, obtuse. Pore surface whitish, daedaleoid. Context whitish, thin (1–1.5 mm), homogeneous, without black lines.
Hyphal system trimitic, generative hyphae hyaline branched with clamp connections, thin-walled, 1.5–2.0 μm in diameter, acyanophilous; skeletal hyphae solid to thick-walled, hyaline, non-septate, 3–4 μm in diameter, totally dominating in the context, acyanophilous, tissues unchanged in KOH, unbranched; binding hyphae very common in both the context and trama, hyaline, thick-walled, acyanophilous, and much branched.
Cystidia absent, but the branches of the binding hyphae may easily be mistaken for thick-walled cystidia in the hymenium unless a careful examination is undertaken. Hyphal pegs present, especially at the base of pores, and regular, 25–30 μm long.
Basidia 12–15 × 3–5 μm, clavate, tetrasterigmatic, sterigmata 3 μm long; Basidioles numerous, similar in shape to basidia but slightly smaller than basidia, up to 4 μm in diameter.
Basidiospores broadly ellipsoid, hyaline, thin-walled, smooth, usually with one guttule each, negative in Melzer’s reagent, acyanophilous, (2.9)3.2–4.6(4.9) × 2.1–2.8(2.9) μm, L = 3.88 μm, W = 2.48 μm; Q = (1.17)1.24–1.91(2.05), Q = 1.57.
Saprotrophic, on dead part of living tree
BENIN. Atlantic province, dry dense forest of Pahou/ Ouidah, leg. Boris A. Olou, on dead wood of
In Benin, seven species of
To place specimens of
Our phylogenetic analyses from
Previously the phylogenetic resolution of
The specimens from Benin identified as members of the
Specimens belonging to the
The sequences belonging to the new species named
The research grant “Bi-nationally Supervised Doctoral Degrees” from the German Academic Exchange Services (DAAD) allowed for the stay of Boris Armel Olou in Germany while carrying out this study.
Names, voucher numbers, and substrates of specimens of
species data
phylogeny data
Support values are given as
phylogeny data
Support values are given as
phylogeny data
Support values given as