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Phylogram generated from maximum likelihood analysis based on combined SSU, LSU, TEF and RPB1 sequence data. Bootstrap values for maximum likelihood (ML, left) and maximum parsimony (MP, right) equal to or greater than 50% and Bayesian posterior probabilities (BYPP, middle) equal to or greater than 0.90 are placed nearby the note. The newly generated sequences are indicated in red bold.
Phylogram generated from maximum likelihood analysis based on ITS sequence data. Bootstrap values for maximum likelihood (ML, left) and maximum parsimony (MP, right) equal to or greater than 50% and Bayesian posterior probabilities (BYPP, middle) equal to or greater than 0.90 are placed nearby the branches, respectively. The newly generated sequences are indicated in red bold and the type species are highlighted in black bold.
Simplicillium formicae (from HKAS 102459, holotype) a superficial hyphae associated with the ant host b–e flask-shaped synnemata f–k phialides bearing conidia l–p conidia. Scale bars: 1000 µm (a); 500 µm (b); 100 µm (d); 30 µm (e, f); 15 µm (j, k); 10 µm (l–p) (e stained with cotton blue solution).
Simplicillium formicae (MFLUCC 18–1379, ex-type living culture) a upper and reverse view of cultures on PDA after 30 days e–g phialides indicated with black arrow c, d, h–j conidial mass on the tip of phialides k–m conidia. Scale bars: 10 µm (c, d, f, g); 20 µm (e); 3 µm (h–j); 1 µm (k–m) (e–j stained with cotton blue solution).
Simplicillium lanosoniveum (a–f from HKAS 102447, g–r from MFLUCC 18–1385) a host (Ophiocordyceps unilateralis); b, c hyphae associated with host indicated with black arrows g, h upper and reverse view of cultures on PDA after 40 days incubation i–l conidial mass on the tip of phialides m–o phialides bearing conidia p–r conidia. Scale bars: 15 µm (i–m); 10 µm (d–f, n, o); 3 µm (p–r) (i, l–n stained with cotton blue solution).
Ophiocordyceps unilateralis (from HKAS 102447) a stroma emerging from host b ascomata on stroma c host (Formicidae) d, e decayed perithecia. Scale bars: 500 µm (b, c); 300 µm (d); 100µm (e).