Corresponding author: Jianchu Xu (
Academic editor: Bao-Kai Cui
Luangharn T, Karunarathna SC, Mortimer PE, Hyde KD, Xu J (2019) Additions to the knowledge of
The genus
Most members of
In Thailand, several
Three specimens of
Morphological characteristics were determined following the methodology described by
Dried internal tissues of the fruiting bodies were used to extract DNA by using the Biospin Fungus Genomic DNA Extraction Kit (BioFlux), following the manufacturer’s instructions. Total reaction mixtures (25 μl) contained 9.5 μl ddH2O, 12.5 μl of PCR master mix, 1 μl of DNA template and 1 μl of each primer (10 μM). The primers used in PCR amplification were: ITS4/ITS5 for internal transcribed spacer gene region (
Sequence data, retrieved from GenBank based on previous studies, are listed in Table
Details of the taxa used in the phylogenetic analysis of this study. The newly generated sequences are in bold.
Fungal species | Voucher | GenBank accession no. | References | |||
---|---|---|---|---|---|---|
|
|
|
RPB2 | |||
|
Cui 13817 |
|
– |
|
|
|
|
Cui 14578 |
|
– |
|
– |
|
|
Dai 12588 |
|
– |
|
– |
|
|
WD 2028 |
|
|
|
|
|
WD 2085 |
|
– |
|
|
|
|
|
DMC 322 |
|
– | – | – |
|
DMC 513 |
|
– | – | – |
|
|
|
Dai16336 |
|
– |
|
|
|
Dai16337 |
|
– |
|
|
|
|
Dai16338 |
|
– |
|
|
|
|
Dai16339 |
|
– |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
ASL799 |
|
|
– | – |
|
PMC126 |
|
|
– | – |
|
|
|
Dai 15970 |
|
– |
|
|
|
Dai 15971 |
|
– |
|
|
|
|
|
Dai 11995 |
|
– |
|
|
|
Yuan 6337 |
|
– |
|
|
|
|
Cui 13982 |
|
– |
|
|
|
|
|
Dai 15601 | – | – | – |
|
|
GDGM 44303 |
|
– | – | – |
|
|
GDGM 40200 |
|
– | – | – |
|
|
|
JV 1008/31 |
|
– |
|
|
|
JV 1008/32 |
|
– |
|
|
|
|
|
K175217 |
|
– |
|
|
|
Cui 14404 |
|
– |
|
|
|
|
Cui 14405 |
|
– |
|
|
|
|
|
Wu1006-38 |
|
– |
|
|
|
Cui 14342 |
|
– |
|
|
|
|
|
LIP SWMart 08-55 |
|
– | – | – |
|
LIP SWMart 08-44 |
|
– | – | – |
|
|
|
UMN7-3 GHA |
|
|
– | – |
|
UMN7-4 GHA |
|
|
– | – |
|
|
|
CWN 04670 |
|
– |
|
|
|
Dai 9447 |
|
– |
|
|
|
|
|
Cui 13918 |
|
– |
|
|
|
|
HMAS86599 |
|
– | – |
|
GenBank |
CBS 194.76 |
|
– |
|
– |
|
|
|
|
– | – | – |
|
|
|
– | – | – |
|
||
|
JV1209/9 |
|
– |
|
- |
|
JV 1209/27 |
|
– |
|
|
|
|
|
HMAS 42798 |
|
– | – | – |
|
CGMCC 5.2175 |
|
– | – | – |
|
|
|
Wei5327 |
|
|
|
|
|
|
Yuan 3490 |
|
– |
|
– |
|
Dai 16434 |
|
– |
|
|
|
|
|
CBS428.84 |
|
– | – | – |
|
|
Dai 16809 |
|
– |
|
|
|
Wei5032 |
|
|
– | – |
|
|
|
FL-02 |
|
– |
|
|
|
FL-03 |
|
– |
|
|
|
|
|
Cui 9011 |
|
– |
|
– |
|
|
TC-02 |
|
– |
|
|
|
MrModeltest v. 2.3 was used to determine the best-fitting substitution model for each single gene partition and the concatenated dataset for Bayesian analyses (
The phylogenetic analyses included 56 taxa (including the three new sequence data) and the tree was inferred from the combined
Phylogram of
Solitary on
THAILAND, Surat Thani Province, Phanom District, Khao Sok national park,
Morphology of
THAILAND, Nakhon Si Thammarat Province, Khanom District, solitary on stump of
The species epithet “
THAILAND, Nakhon Si Thammarat Province, Khanom District, solitary on stump of
Morphological characteristics of
In this study, we describe a new species of
Our findings are consistent with
Micro-morphological characteristics of the context layers of both Guangdong and Thai
According to our phylogenetic analyses (Fig.
We appreciate the kind support given by the Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China; Key Laboratory for Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China and Centre for Mountain Futures (CMF), Kunming Institute of Botany, Kunming 650201, Yunnan, China. We thank the Germplasm Bank of Wild Species, Kunming Institute of Botany, Kunming 650201, Yunnan, China for enabling our molecular laboratory work. Kasiphat Limsakul and Wilawan Punyaboon are acknowledged for their invaluable assistance. Samantha C. Karunarathna thanks the CAS President’s International Fellowship Initiative (PIFI) for funding his postdoctoral research (No. 2018PC0006) and the National Science Foundation of China (NSFC) for funding this work under the project code 31750110478. Peter E. Mortimer thanks the National Science Foundation of China (NSFC) project codes 41761144055 and 41771063 and the South East Asia Biodiversity Resources Institute, CAS, project code Y4ZK111B01. The authors also thank William A. Julian for his editing contributions.