Corresponding author: Wen-Long Zhang (
Academic editor: Cecile Gueidan
Three new species isolated from sugarcane rhizosphere in China, namely
Xie L, Chen Y-L, Long Y-Y, Zhang Y, Liao S-T, Liu B, Qin L-P, Nong Q, Zhang W-L (2019) Three new species of
The genus
All soil samples were collected from the 5–15 cm deep sugarcane rhizosphere by five sampling methods in Guangxi province, China. Fungal isolations were obtained by using Chinese cabbage as a baiting plant, as described by
The genomic DNA was extracted from mycelium grown on PDB (potato dextrose broth) at 28 °C for 10 d using the Prepman Ultra Sample Preparation Reagent Protocol (Applied Biosystems, California, USA). The large subunit ribosomal RNA gene (
Taxa with GenBank accession numbers for
|
|
|
|||
|
|
|
|
||
|
M1 |
|
|
|
|
|
TD2 |
|
|
|
|
|
NN1 |
|
|
|
|
|
LA3 |
|
|
|
|
|
DX4 |
|
|
|
|
|
TD17 |
|
|
|
|
|
M8 |
|
|
|
|
|
CGMCC 14938 |
|
|
|
NS |
|
CGMCC 14939 |
|
|
|
NS |
|
CGMCC 14940 |
|
|
|
NS |
|
MFLUCC 15-0992 |
|
|
|
NS |
|
MFLUCC 17-2349 |
|
|
|
NS |
|
KUMCC 16-0067 |
|
|
|
|
|
CBS 127884 | NS |
|
|
|
|
CBS 138740 | NS |
|
|
|
|
CBS 139032 |
|
|
|
|
|
CBS 132040 |
|
|
|
|
|
MFLUCC 15-0989 |
|
|
|
NS |
|
CBS 132078 |
|
|
|
|
|
ICMP 15131 |
|
|
|
|
|
A 464-3 | NS |
|
|
|
Notes: NS No data in GenBank.
Bayesian analyses of the same aligned four kinds of rDNA sequences dataset were conducted with MrBayes v. 3.1.2 (
The species is named for Nanning City, the type locality.
CHINA. Guangxi: Nanning City, Datang Town.
Colony reached 22 mm diameter on
The new species
In sugarcane rhizosphere soil of southern China.
CHINA. Guangxi: Nanning City, Datang Town.
The species is named for Baise City, the type locality.
CHINA. Guangxi: Baise City, Tiandong County, Silin Town.
Colony reached 14 mm diameter on medium after 2 weeks at 28 °C, grey-white to grey, circular, flat growth, less aerial hyphae, regular edge of colony. Hyphae light yellow-green to light yellow-brown, septate. Conidiophores yellow-brown, mostly stubby, 0–2-branched, 0–8-septate, straight or flexuous, 3–12 × 2–6 μm (7 ± 2 × 4 ± 1 μm,
The new species
In sugarcane rhizosphere soil of southern China.
CHINA. Guangxi: Baise City, Tiandong County, Silin Town.
The epithet “
CHINA. Guangxi: Chongzuo City, Daxin County, Lanxu Village.
Colony reached 15 mm diameter on medium after 2 weeks at 28 °C, greywhite to grey, circular, flat growth, less aerial hyphae, regular edge of colony. Hyphae light yellow to yellow-brown, septate. Conidiophores yellow-brown, mostly stubby, 0–2-branched, 0–6-septate, straight or flexuous, 3–30×2–4 μm (10 ± 7 × 3 ± 1 μm,
The new species
In sugarcane rhizosphere soil of southern China.
CHINA. Guangxi: Nanning City, Long’an County, Natong Town.
To determine the phylogenetic positions of the three new species,
Bayesian tree based on the combined
The genus
This work was supported by China National Natural Science Foundation (No. 31460016), Guangxi Natural Science Foundation (No. 2015GXNSFBA139083), and Basic Scientific Research Special Project of Guangxi Academy of Agricultural Sciences (No. 2015YT80).