Phylogeny and species delimitations in the entomopathogenic genus Beauveria (Hypocreales, Ascomycota), including the description of B. peruviensis sp. nov.

Abstract The genus Beauveria is considered a cosmopolitan anamorphic and teleomorphic genus of soilborne necrotrophic arthropod-pathogenic fungi that includes ecologically and economically important species. Species identification in Beauveria is difficult because of its structural simplicity and the lack of distinctive phenotypic variation. Therefore, the use of multi-locus sequence data is essential to establish robust species boundaries in addition to DNA-based species delimitation methods using genetic distance, coalescent, and genealogical concordance approaches (polyphasic approaches). In this regard, our study used multilocus phylogeny and five DNA-based methods to delimit species in Beauveria using three molecular makers. These polyphasic analyses allowed for the delimitation of 20–28 species in Beauveria, confirming cryptic diversity in five species (i.e. B. amorpha, B. bassiana, B. diapheromeriphila, and B. pseudobassiana) and supporting the description of B. peruviensis as a new taxon from northeastern Peru. The other five species were not evaluated as they did not have enough data (i.e. B. araneola, B. gryllotalpidicola, B. loeiensis, B. medogensis, and B. rudraprayagi). Our results demonstrate that the congruence among different methods in a polyphasic approach (e.g. genetic distance and coalescence methods) is more likely to show reliably supported species boundaries. Among the methods applied in this study, genetic distance, coalescent approaches, and multilocus phylogeny are crucial when establishing species boundaries in Beauveria.


Introduction
Around 1800, a silkworm disease called "calcine", "real del segno" or "muscardine" was causing great trouble in Italy and France (Redaelli and Visocchi 1940). Experiments developed by Agostino Bassi in Mariago, Italy showed that a parasitic fungus produced this disease (Redaelli and Visocchi 1940). Balsamo (1835) confirmed this discovery and concluded that the incrustation and white efflorescence, which covered the body of a dead silkworm, were a fungus of the genus Botrytis. He first named this species Botrytis paradoxa Balsamo and later Botrytis bassiana Balsamo (Balsamo 1835). Then, this species was transferred to its own genus and Beauveria Vuillemin was established on the basis of B. bassiana Vuillemin as the type species (Vuillemin 1912).
The genus Beauveria is considered a cosmopolitan genus of soilborne necrotrophic arthropod-pathogenic fungi that includes ecologically and economically important species (Rehner et al. 2011, Kepler et al. 2017, Chen et al. 2018. Morphologically, Beauveria genus have been characterized asexually by having conidiogenous cells arising from short, often one-celled, more or less swollen stalk cells, often in dense clusters, or scattered or in whorls from undifferentiated hyphae; they consist of a globose to fusiform basal part, and a geniculate, denticulate rachis. Conidia one-celled, hyaline, smooth, thin-walled, globose to ellipsoidal ( de Hoog 1972). The sexual morphs form stromata solitary, paired or gregarious, unbranched, fleshy texture, fertile area apical, cylindrical to clavate, yellowish to orange; perithecia partially immersed, in longitudinal section oval to ovoid; and asci hyaline with cylindrical and filiform ascospores (Kepler et al. 2017).
Based on the end of dual nomenclature for different morphs of the same fungus in 2011 (McNeill et al. 2012), Kepler et al. (2017) phylogenetically established the genetic boundaries in Cordycipitaceae regardless of life-stage or the associated morphological differences. One of the most significant changes was the recognition of Beauveria as a genus separate from Cordyceps. Although direct links between species of Beauveria and cordyceps-like sexual morphs have been demonstrated from molecular data and culture-based experiments (Shimazu et al. 1988, Li et al. 2001, Huang et al. 2002, Shrestha et al. 2014, their respective type species are not congeneric (Kepler et al. 2017). Thereby, the clade composed of Beauveria currently includes the traditional species known from asexual morphs, as well as several taxa previously described for sexual morphs in Cordyceps (Sanjuan et al. 2014, Kepler et al. 2017. Initially, Beauveria was delimitated based on diagnostic features, and three species were recognized, i.e., B. bassiana, B. brongniartii and B. alba (Limber) Saccas ( de Hoog, 1972). New additions were included by de Hoog and Rao (1975), Samson and Evans (1982), Bissett and Widden (1986) and Rehner et al. (2006). Molecular analyses confirmed the monophyly and placement of seven species of Beauveria within Cordycipitaceae Buckley 2005, Sung et al. 2007). More recent molecular studies based on multilocus phylogenetic analysis that included the Bloc nuclear intergenic region, internal transcribed spacer (ITS), translation elongation factor-1α (TEF), and RNA polymerase II largest subunit (RPB1) and second largest subunit (RPB2) demonstrated that Beauveria is composed of 26 species (Rehner et al. 2011, Sanjuan et al. 2014, Kepler et al. 2017, Chen et al. 2018. These phylogenetic studies also revealed that the most commonly reported species, namely, B. bassiana and B. brongniartii, encompass cryptic lineages with worldwide distributions (Rehner et al. 2006, Ghikas et al. 2010. Although morphologically distinctive as a genus, species identification in Beauveria, especially in the conidiogenic state, is difficult because of its structural simplicity and lack of distinctive phenotypic variation. Thus, numerous registered mycoinsecticide formulations based on B. bassiana and B. brongniartii that are extensively used for the control of insect pests worldwide (Faria and Wraight 2007) are not likely based on these species (Rehner et al. 2006).
In the Amazonian region, a total of five species have been reported (Rehner et al. 2011, Sanjuan et al. 2014 (Sanjuan et al. 2014). Additionally, two species of Beauveria were reported from Peru: B. amorpha Samson & Evans and B. bassiana, but only the former has been confirmed by molecular analysis while the latter is extensively used in coffee rust programs to control the expansion of the coffee borer (Rehner et al. 2011).
Given the problems with species delimitation in fungi using morphology, molecular data are becoming the standard for delimiting species and testing their traditional boundaries (Rehner et al. 2011). The recognition of distinct clades in gene trees as species is likely to be misleading in understanding the evolutionary history of taxa (Lu et al. 2016). Therefore, the use of multi-locus sequence data is essential to establish robust species boundaries (Lumbsch and Leavitt 2011). Most researchers, however, did not carefully examine the species boundaries but simply recognized distinct clades in singlegene trees as separate species (Stewart et al. 2014). Estimating the species tree and species delimitation using genetic distance (e.g. automated barcode gap discovery algorithm, ABGD; and statistical parsimony, SPN), coalescent (e.g. generalized mixed Yule coalescent, GMYC; and Bayesian phylogenetics and phylogeography, BPP), and genealogical concordance (genealogical concordance phylogenetic species recognition, GCPSR) methods have proven very useful and have been used for a range of animal and plant taxa (Liu et al. 2016). These methods have otherwise not been used much in fungi, especially in studies of pathogenic fungi (Millanes et al. 2014, Liu et al. 2015. Therefore, the use of several methodologies and data sets to delimit species is recommended, and subsequently, the achievement of congruent results across the methods is likely to prove most useful for framing reliably supported species boundaries (Carstens et al. 2013).
In this study, we analyzed species of the newly circumscribed genus Beauveria, including an unreported species isolated from coffee farms in northeastern Peru, based on morphological observations, phylogenetic inferences, and DNA-species delimitation meth-ods. Three nuclear molecular markers (Bloc, rpb1, and tef1) were used to examine their phylogenetic relationships and to assess species boundaries within the genus Beauveria.

Collection of specimens and isolation
Fungal strains were isolated from infected coffee borers (Hypothenemus hampei) obtained from infected coffee berries according to Gerónimo-Torres et al. (2016). They were collected during July and August 2017 from three districts in the province of Rodriguez de Mendoza, Amazonas, Peru (Fig. 1). Briefly, infected coffee berries were preserved at 5 °C until coffee borers were recovered from them. The coffee borers with signs of fungal infection were cleaned superficially in 0.5% sodium hypochlorite solution and rinsed with sterile distilled water. Then, insects were placed in a humid chamber (90% RH and 25 °C) for 8 days to allow the growth of the entomopathogenic fungus. Once visible mycelia appeared on the borers under observations with a stereo microscope (Nikon SMZ18, Tokyo, Japan), these were transferred to a Petri-dish containing potato dextrose agar (PDA; Merck, Darmstadt, Germany).

Identification of isolates
Fifty-five fungal strains were incubated as monosporic cultures on PDA at 25 °C for 15 days. Morphological characterization of the fungus was performed as described by Rehner and Buckley (2005). Microscope observations were made from fungal mycelia and other structures stained with methylene blue (0.1-0.5%). Photomicrographs were taken under an inverted microscope (IX83; Olympus, Tokyo, Japan) with an integrated camera (Nikon D810, Tokyo, Japan). Fungal strains were deposited as semisolid and dry material in the herbarium of Toribio Rodriguez de Mendoza National University (UT), Peru.

Molecular phylogenetic analyses
Genomic DNA was extracted from semisolid PDA cultures using the NucleoSpin Plant II Kit (Macherey-Nagel, Düren, Germany), following the manufacturer's instructions. Three genes were sequenced, i.e., Bloc, rpb1, and tef1. Each gene was amplified using polymerase chain reaction (PCR) with MasterMix (Promega, Wisconsin, USA) in the following reaction mixture: 10 ng of DNA and 0.25-0.5 pmol of forward and reverse primers for a total volume of 10 μl. The PCR protocols and primer combinations for Bloc (B5.1F, B5.4F, B3.1R, B3.3R), rpb1(RPB1A, RPB1A_VH6R, RPB1B_VH6Fa, RPB1B_G2R), and tef1 (983F, 1567RintB) followed Rehner et al. (2011). The se- quences of the forward and reverse strands were determined commercially by Macrogen Inc. (Macrogen, Seoul, Korea). New Bloc, rpb1, and tef1 sequences were deposited in GenBank (Table 1). These sequences and others obtained from GenBank were initially aligned with Muscle algorithms (Thompson et al. 1994) and were adjusted manually with MEGA6 software (Tamura et al. 2013).  The phylogeny was based on concatenated data combining Bloc, rpb1, and tef1 (101 sequences, Table 1). Selection of the best-fitting nucleotide substitution model was conducted using the program PartitionFinder (Lanfear et al. 2012) with three partitions (Bloc, rpb1, and tef1). The best partition strategy and model of sequence evolution were selected based on the Bayesian Information Criterion (BIC). The general time reversible nucleotide substitution model with a gamma distribution and a proportion of invariable sites (GTR + Γ + I) was selected for all partitions. Maximum likelihood (ML) analyses were conducted with the RAxML HPC-AVX program (Stamatakis 2014) implemented in the raxmlGUI 1.3.1 interface (Silvestro and Michalak 2012) using a GTRGAMMAI model with 1000 bootstrap replications. Bayesian inference (BI) was performed with MrBayes v. 3.2.6 software (Ronquist et al. 2012) using Metropolis-coupled MCMC and the GTR + Γ + I model. We conducted two runs each with four chains (three hot and one cold) for 10,000,000 generations, sampling trees every 1,000 generations. We plotted likelihood vs. generation using the Tracer Version v. 1.6 program (Rambaut et al. 2014) to reach a likelihood plateau and set the burn-in value.

DNA-based species delimitation
Although 26 species have been molecularly confirmed in Beauveria (Rehner et al. 2011, Kepler et al. 2017, Chen et al. 2018, only 21 of these species and Beauveria sp. Shenoy were not used due to abundant missing data and short sequences for the three markers (e.g. ~731 bp for rpb1 and ~720 bp for tef1).
To perform the GMYC delimitation method, an ultrametric tree was constructed in BEAST v.2.0.2 (Drummond et al. 2012), relying on the uncorrelated lognormal relaxed clock, the GTR + Γ + I model, and a coalescent tree prior. Bayesian Markov chain Monte Carlo was run for 50 million generations, and trees and parameters were sampled every 1000 generations. Log files were visualized in Tracer v.1.6 (Rambaut et al. 2014) for assessing the stationary state of parameters on the basis of the value of estimate-effective sample size (ESS). After removing 25% of trees as burn-in, the remaining trees were used to generate a single summarized tree in TreeAnnotator v.2.0.2 (part of the BEAST v.2.0.2 package) as an input file for GMYC analyses. The GMYC analyses with a single threshold model were performed in R (R Development Core Team, http://www.R-project.org) under the 'splits' package using the 'gmyc' function (R-Forge, http://r-forge.r-project.org/projects/splits/).
To validate the outcomes of single locus species delimitation, a multilocus BPP was applied using the program BP&P v.2.0 (Rannala and Yang 2003, Yang and Rannala 2010, Liu et al. 2015. The three-gene data (Bloc, rpb1, and tef1) were used as input for BPP under the A11 model (A11: species delimitation = 1, species tree = 1). Specimens were a priori assigned to species based only on the minimum number of species from the results of the phylogenetic analysis. The guide tree derived from the three-gene ML analysis was used. Five variables (ε1~ε5) were automatically fine-tuned following the instructions of BP&P Yang 2003, Yang andRannala 2010). The prior distribution of θ and τ could have influenced the posterior probabilities for different models (Yang and Rannala 2010). Analyses were run with three different prior combinations (Leaché and Fujita 2010). Each analysis was run three times to confirm consistency between runs. Two independent MCMC analyses were run for 100,000 generations with the 'burn-in' = 20,000. GCPSR was implemented by identifying independent evolutionary lineages (IELs) and by exhaustive subdivision of strains into phylogenetic species. The criteria used to identify IELs and exhaustive subdivision were the same as those used by Brankovics et al. (2018). These were implemented using Perl scripts developed by Brankovics et al. (2018) and available at GitHub (https://github.com/b-brankovics/GCPSR).

Molecular phylogeny
In the phylogeny of Beauveria species, the analyzed data matrix included 1592 base pairs (bp) for Bloc, 2890 bp rpb1, and 1181 bp for tef1 of 101 individuals. Phylogenetic trees obtained from ML and BI analyses confirmed the robustly supported monophyly of the genus Beauveria (Fig. 2). The tree topologies for the individual genes (tef1, Bloc, and rpb1) did not show congruence (Suppl. material 1: Figs S1-S3). These trees showed topological differences, especially in the clades composed of B. asiatica / B. majiangensis and by B. bassiana / B. staphylinidicola / Beauveria sp. from Peru. Although the individual gene trees did not show congruence with the combined data, the latter resolved these clades, suggesting conspecificity in the first clade and sister relationship in the second. Moreover, the multilocus phylogeny showed well-supported clades in both the ML and BI analyses except in B. lii, B. majiangensis, and B. staphylinidicola. The genetic divergence comparisons showed that the minimum threshold (p-distance) to distinguish genetic species in Beauveria was 1.3%, 0.4%, and 0.2% for Bloc, rpb1, and tef1, respectively, as occurred between B. australis and B. asiatica.( Table 2).  x = non recognized as species, -= not evaluated, * = posterior probabilities higher or equal than 0.53, *** =highly significant

Species delimitation
The species-delimitation methods based on genetic distance (ABGD, SPN), coalescence (GMYC, BPP), and genealogical concordance (GCPSR) showed incongruent results for the three genes (Fig. 2, Table 3). Among these methods, the highest number of species was delimited in the GMYC analysis for the Bloc gene, whereas conservative results were observed in BPP. The species delimitations by SPN and GCPSR have inadequate and contradictory results. The genetic distance method based on the barcode gap (ABGD) found similar species numbers for Bloc, rpb1, and tef1, differing only in the species recognized in the clades B. asiatica / B. majiangensis and B. bassiana / B. staphylinidicola / Beauveria sp. from Peru. In the former clade, there were 3, 1 and 2 species for Bloc, rpb1, and tef1; whereas in the latter clade, there were 7, 1, and 5 species for Bloc, rpb1, and tef1. The GMYC identified relatively conserved results in RPB1 (28) and tef1 (26) and plenty of species in the Bloc data set (63). This high number of species for the Bloc data set is a consequence of the splitting of the main clades into different species but lacking significance (Suppl. material 1: Table S1, Fig. S4). Regarding the multi-locus coalescent species validation (BPP), the highest posterior probabilities for Bloc, rpb1, and tef1 were found by recognizing 16 species based on the results from the phylogenetic analysis and single species delimitation methods (Suppl. material 1: Table S2). Conversely, the BPP analyses with the maximum number of species (39 and 62) were not used based on the inadequate results from SPN and GCPSR. Although there were incongruent results among different methods, the conservative results from species delimitation methods (ABGD and GMYC) and phylogenetic analysis suggest that Beauveria is composed of 20-28 and 26 species, respectively. These results also suggest that the clade composed of B. asiatica / B. majiangensis, B. diapheromeriphila, and B. bassiana / B. staphylinidicola / Beauveria sp. from Peru were genetically composed of more than one species. Our analysis also revealed that Beauveria sp. from Peru was supported as a distinct species by ABGD (Bloc gene), GMYC, BPP, and phylogeny. Thereby, the description of Beauveria sp. as a new species is proposed. Diagnosis. Species very similar morphologically to Beauveria bassiana, but differing in the sister phylogenetic relationship with this species (Fig. 2). The sequence divergence between B. peruviensis and B. bassiana is 3.5-4.1% for Bloc, 0.3-0.5% for rpb1, and 0.2-0.4% for tef1. B. peruviensis is occurring in coffee plantations located in the middle altitudes of the Amazon region of Peru. Type. PERU. Amazonas: Prov. Rodríguez de Mendoza, Dist. Huambo, latitude -6.469, longitude -77.376, elev. 1642 m, entomopathogenic, 08 Nov. 2017, G. Ángulo, UTRP19 (holotype: UFV5609; isotype: ARSEF14196).
Distribution. This species is widely spread on coffee plantations in the middle altitudes of the Amazon region in northeastern Peru.
Ecology. B. peruviensis was isolated from coffee borers (Hypothenemus hampei) obtained from coffee grains. Only the asexual stage was found.
Etymology. The specific epithet 'peruviensis' is derived from the country where the samples were collected. Notes. Beauveria peruviensis is practically indistinguishable in morphology to other Beauveria species. The shape and size of the conidia and the colony color of B. peruviensis among other morphological features have been observed in B. bassiana, B. kipukae, B. pseudobassiana, and B. varroae (Rehner et al. 2011). The lack of diagnostic morphological features to distinguish Beauveria peruviensis was overcome by delimiting this species with DNA-based methodologies.

Discussion
Accurate species identification within the entomopathogenic fungi Beauveria is crucial for disease control and prevention (Lu et al. 2016). This genus has recently been circumscribed, and its taxonomy has been updated with new combinations and the description of new species based mainly on multi-locus phylogenies in the absence of diagnostic features that delimit species (Sanjuan et al. 2014, Shrestha et al. 2014, Kepler et al. 2017, 2018. In addition to phylogenies, other methodologies and data sets to delimit species are recommended to establish well-supported boundaries among species (Carstens et al. 2013) because most researchers simply recognize distinct clades in either single-or multi-locus trees as species (Stewart et al. 2014). In this regard, our study used phylogeny and five DNA-based methods to delimit species in Beauveria using three molecular makers. Although incongruence among some of these methods was observed in our analyses, a genetic distance (ABGD), a coalescence method (BPP), and the multilocus phylogeny strongly supported 20-28 different species, including the new species B. peruviensis from Peru.
The use of multi-locus sequence data is essential to establish robust species boundaries (Lumbsch and Levitt 2011), and our results for Beauveria showed well-supported clades, although it resulted in incongruence to the single locus phylogenies (Suppl. material 1: Figs S1-S3). This conflict can be a result of incomplete lineage sorting, horizontal gene transfer, gene duplication and loss, hybridization, or recombination (Degnan and Rosenberg 2009). This study cannot determine which of these scenarios are occurring in Beauveria; nevertheless, it serves as a baseline for investigating causes of gene tree discordance that can be identified by further analyses at the genomic level (Patterson et al. 2006, Lu et al. 2016). According to our multilocus phylogeny, 22 of the 26 molecularly confirmed species in Beauveria were recognized. Previous studies have delimited B. araneola, B. gryllotalpidicola, B. loeiensis, B. medogensis, and B. rudraprayagi as valid species on the basis of their phylogenies (Agrawal et al. 2014, Imoulan et al. 2016); however, our study did not include these sequences because they have abundant missing data, and thus, their status was not evaluated. These species would require further revision to be recognized as supported lineage within the genus Beauveria.
Regarding the genetic distance methods, the ABGD showed similar results when delimiting Beauveria species to those from the multilocus phylogeny. The additional putative species in ABGD is mainly due to the split of B. bassiana. This confirms that B. bassiana encompasses cryptic lineages as proposed initially by Rehner et al. (2011). Therefore, the original B. bassiana should be the clade that includes the specimen from the type locality, namely, Italy (Vuillemin 1912). Additionally, these results delimited B. majiangensis and B. asiatica as different lineages, although the multilocus analysis showed low support. B. majiangensis needs further analysis with additional and longer sequences to confirm its status because one or only a few individuals often fail to represent the species as a whole (Davis andNixon 1992, Walsh 2000). On the other hand, the SPN method showed conflicting results among the Bloc, RPB1, and tef1 loci, leading to incorrect inferences. The number of species inferred by SPN greatly matched the number of Linnaean species in mitochondrial markers (e.g., COI) (Hart and Sunday 2007). Therefore, our nuclear markers due to indels might generate many reticulations that allow inadequate species delimitation in our data (Paradis 2018).
In the coalescence methods, although 6 species were not included in the BPP analysis due to the lack of their Bloc sequences, this method supports the conservative results obtained from the multilocus phylogeny. BPP supported the status of 16 species (posterior probabilities higher than 0.52), which are not high supportive, but these probabilities are not supportive at all when splitting or merging species in the BPP analysis (Suppl. material 1: Table S2). Zhang et al. (2011) found that the correct species model was inferred with a high posterior probability with only one or two loci when 5 or 10 sequences were sampled from each population or with 50 loci when only one sequence was sampled, and they also demonstrated that the migration rate might affect these results. This suggests that further analysis might need to increase the number of sequences per locus among different populations of species of Beauveria and assess their migration rate to obtain supportive delimitations. Moreover, the highly significant results obtained from the GMYC method for the tef1 and rpb1 loci partially support the ABGD and multilocus analyses. The additional number of putative species in the GMYC analyses, as occurred with the ABGD, is due to the presence of more than one lineage in B. amorpha, B. bassiana, B. diapheromeriphila, and B. pseudobassiana confirming cryptic diversity (Rehner et al. 2011). The performance in empirical studies of the ABGD and GMYC tends to under-and oversplit species, respectively (Luo et al. 2018). However, our results suggest that GMYC and ABGD are appropriate for determining cryptic diversity in Beauveria by splitting well-supported clades from the multi-locus phylogeny.
Regarding B. peruviensis, ABGD (Bloc), SPN (Bloc), GMYC, BPP, and the phylogenetic analyses support this species as a different lineage from B. bassiana and B. staphylinidicola. Additionally, the genetic divergence between B. peruviensis and these species is higher than the minimum threshold observed in species of Beauveria (Table 2). In our study, Beauveria peruviensis showed morphological indistinctiveness to other Beauveria species that produce globose/subglobose/ellipsoid conidia. Additionally, B. peruviensis conidia is also similar in size to other Beauveria, especially B. bassiana. Previously, Rehner et al. (2011) noted that B. bassiana is hardly distinguishable from other species of Beauveria. The lack of diagnostic morphological features to delimit species in Beauveria was overcome by the application of molecular methods in fungal taxonomy. The segregation of B. peruviensis from B. bassiana and B. staphylinidicola confirmed that phylogenetic diversity and DNAspecies delimitation methods discover taxa within morphologically defined species (Goldstein et al. 2000, Lu et al. 2016. Ecologically, the segregation of B. peruviensis from B. bassiana and B. staphylinidicola is supported by the specificity of B. peruviensis to the coffee borer from Amazon and the well-supported lineage in the phylogenetic analysis that might indicate the presence of a barrier in gene flow in nature (Van Valen 1976, Lu et al. 2016. Recently, polyphasic approaches have been used to reflect the natural classification of species within many important fungal genera (Aveskamp et al. 2010, Milic et al. 2012, Lu et al. 2016. These approaches frequently incorporate morphological and phylogenetic analyses and metabolomics, but few of them use genetic distance and coalescent methods (Lu et al. 2016). The use of polyphasic analysis, including DNA-based delimitation methods, allowed the establishment of boundaries among species of morphologically conserved genera such as Beauveria and thus provided support for the description of new taxa (e.g., B. peruviensis) or validated the taxonomic uncertain of others (e.g., B. majiangensis). Although more recent methods avoid arbitrary cut-offs (Knowles and Carstens 2007), our results demonstrate that the congruence among this method and other methods used in a polyphasic approach (e.g., genetic distance, coalescence methods) are more likely to prove reliably supported species boundaries (Carstens et al. 2013). Among the methods applied in this study, ABGD, GMYC, BPP, and multilocus phylogeny are crucial when establishing species boundaries in Beauveria.