Corresponding author: Shu-Yan Liu (
Academic editor: N. Wijayawardene
A powdery mildew (
Qiu P-L, Braun U, Li Y, Liu S-Y (2019)
The family
In 2018, leaves of
In May 2018,
Genome DNA was extracted using chasmothecia of
The newly obtained sequence data (28S rDNA, including domains D1 and D2 and ITS, including the 5.8S rDNA) from two powdery mildew specimens on
Vouchers, hosts and GenBank accession numbers of the sequences used in this study.
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MUMH 171 |
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1326 |
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MUMH 2741 |
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1335 |
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MUMH 3620 |
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1335 |
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MUMH 0002 |
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1317 |
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HMJAU91777 |
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671 |
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637 | This study | |||||
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HMJAU91771 |
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670 |
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637 | This study | |||||
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666 |
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636 | ||||||
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666 |
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636 | ||||||
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MUMH 2193 |
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1403 |
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MUMH 2484 |
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1355 |
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MUMH 4644 |
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1314 |
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MUMH 0087 |
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1362 |
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MUMH 0514 |
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1361 |
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MUMH 4651 |
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1350 |
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TPU 1745 |
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1276 |
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MUMH 2987 |
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552 |
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754 | ||||||
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MUMH 412 |
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1516 |
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MUMH 0099 |
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1351 |
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MUMH 2576 |
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1321 | ||
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MUMH 4642 |
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1356 |
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MUMH 0071 |
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1249 |
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MUMH 0066 |
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1344 |
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A phylogenetic tree was obtained from the combined data using the maximum-parsimony (MP) method in PAUP 4.0. The MP analysis was performed with the heuristic search option using the tree-bisection-reconstruction (TBR) algorithm with 100 random sequence additions to find the global optimum tree. The gaps were treated as missing data. The bootstrap analysis (1000 replications) was used for testing the strength of the internal branches of the resulting trees (
The alignments of ITS and 28S rDNA sequences obtained from the two specimens examined are identical to each other. A total of 22 combined sequence data, including sequences from
Maximum parsimony phylogram of
Named after the host genus,
Differs from all known
CHINA. Beijing City, Baihua Mountain, on leaves of
Forming distinct white colonies, very small and dense, covering both sides of the leaves, causing discolourations of entire leaves or even malformations. Mycelium amphigenous, effuse and persistent. Hyphal appressoria distinctly lobed, solitary (Figure
Morphology of
On
For taxonomic purposes within the genus
The phylogenetic analysis revealed that
Morphological comparison of
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18.6‒35.6 × 10.2‒14.1 | 54.7‒171.0 (‒234.7) | 71.0‒100.0 | 6‒14 | mycelioid | 4‒6 | 5‒8 | colourless |
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‒ † | ‒ | (85–) 95–120 | 10–40 | mycelioid | 4–8 | 6–8 | yellowish |
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35–45 × 17–23 | 80–110 | 90–130 | 18–36 | mycelioid | 6–16 | 2–3 | colourless |
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25–35 (–40) × (16.5–) 17.5–20 (–22) | 50–75 | 70–150 | 4–16 | dichotomous | 2–6 | 4–8 | colourless |
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‒ | ‒ | 120–225 | 19–40 (–48) | circinate | (5–) 6–12 (–21) | (4–) 5–8 | colourless |
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26–33 × 13–18 | ‒ | 75–110 | 5–20 | mycelioid | (3–) 4–5 (–6) | 5–8 | colourless |
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27–38 × 14–18 | up to 90 | 80–120 | 7–22 | circinate | 6–13 | 4–5 | colourless |
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average 26.5 × 14 | 45–80 | average 120 | 5–23 | circinate | (3–) 4–9 (–11) | (2–) 5–7 (–8) | yellowish |
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(18–) 25–40 (–45) × (9–) 12–19 (–22) | 40–130 (–175) | ‒ | ‒ | ‒ | ‒ | ‒ | ‒ |
† “‒” means no related information
The authors sincerely acknowledge Prof. Susumu Takamatsu for reading the full text, for critical comments and for corrections. We appreciate the laboratories of Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi and College of Plant Protection in Jilin Agricultural University, Changchun, China. This work was supported by the National Natural Science Foundation of China [grant programme numbers 31670022, 31470153].