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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Material and methods
    • DNA extractions
    • Amplification, normalisation and pooling
    • Library preparation, sequencing and SMRT Portal primary analysis
    • Molecular identification and validation
  • Results
    • Amplifications
    • Sequencing
    • Sequence identity
    • Validation
  • Discussion
    • Technical considerations for high-throughput DNA extraction and amplicon library preparation
    • Long Amplicon Analysis and the recovery of high-quality sequences for the target lichenised fungal species
  • Conclusion
  • Acknowledgment
  • References
  • Supplementary material
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