Corresponding author: Jichuan Kang (
Academic editor: T. Lumbsch
Two new endophytic species,
Zhou S, Qiao L, Jayawardena RS, Hyde KD, Ma X, Wen T, Kang J (2019) Two new endophytic
Endophytic fungi were isolated from different parts of
In this study, we introduce two novel species,
Fresh healthy plant samples (leaves, stems and roots) of
Each part of the plant was surface sterilised to eliminate epiphytic microorganisms. The samples were washed thoroughly in running tap water, followed by immersion in 70% (v/v) ethanol for 3 min to sterilise the surfaces, then rinsed with sterilised distilled water for 1 min. Samples were dried on sterilised filter paper and then placed in 3% hydrogen peroxide for 7 min, washed in sterilised distilled water and dried on a sterilised filter paper again. Each plant tissue was then cut into small cubes (0.5 × 0.5 cm) using a sterilised blade. The cubes were placed on potato dextrose agar (
The type specimens are deposited in
Genomic DNA was extracted from fresh fungal mycelia using the BIOMIGA Fungus Genomic DNA Extraction Kit (GD2416, Biomiga, USA), following the manufacturer’s instructions. DNA samples were stored at -20 °C until used for polymerase chain reaction (
Sequence data of the four loci were blasted in the GenBank database and all top hits, including the corresponding type sequences, were retrieved (Table
Taxa used for phylogenetic analyses in the study.
Species name | Isolate No.b | GenBank Accession No. | |||
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TUB | ||
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AR3920 |
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–a | – | – |
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LC1041 |
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SXC151* |
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AGMy0254* |
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CAUG17* |
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ICMP18579* |
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– |
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– | – | |
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– |
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C1266.1 |
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– |
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– | |
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CAUOS2* |
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– | – | – | |
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L58 |
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Notes: New strains are in bold. * ex-type strains.
Maximum Likelihood (
For Bayesian Inference (
Isolates were grown on
Four hundred and forty endophytic fungi were isolated from different parts of
Phylogenetic analysis of four loci (
Phylogram generated from Maximum Likelihood (RAxML) analysis based on combined
Representatives of complexes and species in
‘
Endophytic fungus in root of
Colonies on
CHINA, Hunan Province, Jishou City (
China, Hunan Province, Jishou City (
‘
Endophytic in leaves and stems of
Cultures on
Colonies on
Cultures on
CHINA, Guizhou province, Tongren (
Morphological features and genes sequence data are recognised as a basis for describing new species, but sometimes morphological features of
The composition of endophytic microorganisms may depend on the plant age, tissue, host type and time of isolation (
This work was funded by grants of the National Natural Science Foundation of China (NSFC Grants nos. 31670027 &31460011 & 30870009). Sixuan Zhou thanks Dr. Shaun Pennycook, Prof. Jiangming Lv, Yongzhong Lu and Jianfei Gao for their help.