Most likely tree based on maximum likelihood phylogenetic inference showing the placement of Tuber incognitum within the Puberulum clade. Bootstrap values ≥ 70% are labelled above nodes. Nodes with posterior probabilities ≥ 99% are blacked. Holotype collections are labelled. The phylogeny is rooted with species belonging to the Maculatum clade. Scale bar corresponds to the mean number of nucleotides substitutions per site.