Map of the Kruger National Park (KNP) in South Africa. The arrows indicate the location of the four research “supersites” (adapted from Smit et al. 2013). Sampling site is indicated with a black star. The inset shows the location of the KNP within South Africa, indicated by a grey box.

Maximum-likelihood (ML) phylogram obtained from combined EF-1α, RPB1 and RPB2 sequences of 18 strains belonging to the Fusarium buharicum (FBSC), Fusarium tricinctum (FTSC) and Fusarium lateritium (FLSC) species complexes. Numbers on the nodes are ML bootstrap values above 70% and Bayesian posterior probability values above 0.95. Branch lengths are proportional to distance. Ex-type strains are indicated with ^T. Strains corresponding to new species described here are shown in bold.

Maximum-likelihood (ML) phylogram obtained from combined CAL, EF-1α, RPB1, RPB2 and TUB sequences of 48 strains belonging to the Fusarium fujikuroi (FFSC) and Fusarium oxysporum (FOSC) species complexes. Numbers on the nodes are ML bootstrap values above 70% and Bayesian posterior probability values above 0.95. Branch lengths are proportional to distance. Ex-type, ex-neotype and ex-paratype strains are indicated with ^{T, NT} and ^PT, respectively. Strains corresponding to new species described here are shown in bold.

Maximum-likelihood (ML) phylogram obtained from combined RPB1 and RPB2 sequences of 35 strains belonging to the Fusarium sambucinum (FSAMSC) and Fusarium fujikuroi (FFSC) species complexes. Numbers on the nodes are ML bootstrap values above 70% and Bayesian posterior probability values above 0.95. Branch lengths are proportional to distance. Ex-type strains are indicated with ^T. Strains corresponding to new species described here are shown in bold.

Fusarium convolutans sp. nov. A–D Colonies on PDA, SNA, OA and CMA, respectively, after 7 d at 24 °C in the dark E–IConidiophores, phialides and conidia J–MChlamydosporesN–P Sterile hyphal projections Q Conidia. Scale bars: 20 μm (E, F); 5 μm (G–I); 10 μm (J–Q).

Fusarium fredkrugeri sp. nov. A–D Colonies on PDA, SNA, OA and CMA, respectively, after 7 d at 24 °C in the dark E–GSporodochia formed on the surface of carnation leaves H–N Aerial conidiophores, phialides and conidia O, P Aerial conidia Q Sporodochial conidiophores and phialides R Sporodochial conidia. Scale bars: 100 μm (E–G); 10 μm (H–R).

Fusarium transvaalense sp. nov. A–D Colonies on PDA, SNA, OA and CMA, respectively, after 7 d at 24 °C in the dark E Pustule-like growth on OA F, GSporodochia formed on the surface of carnation leaves H–L Aerial conidiophores phialides and conidia M Aerial conidia N, OChlamydosporesP Sporodochial conidiophores and phialides Q Sporodochial conidia. Scale bars: 2 mm (E); 20 μm (F–J); 5 μm (K); 10 μm (L–Q).