Corresponding author: Yu Pei Tan (
Academic editor: C. Gueidan
Several unidentified specimens of
Tan YP, Crous PW, Shivas RG (2018) Cryptic species of
Species delimitation within
In Australia, 64 species of
In this study, 17 unidentified isolates of
Unidentified isolates of
|
Isolate no.1 |
|
Location | GenBank accession numbers2 | ||
---|---|---|---|---|---|---|
ITS |
|
|
||||
|
CBS 136.29 T |
|
USA |
|
|
|
|
CBS 294.61 T | air | Brazil |
|
|
– |
|
CBS 154.34 T | unknown | Indonesia |
|
|
|
|
CBS 317.86 | unknown | Japan |
|
|
|
|
BRIP 16080 T | unknown | India |
|
|
|
|
MFLUCC 10-0703 T |
|
Thailand |
|
|
|
|
UTHSC 08-3414 T |
|
USA |
|
|
– |
|
MFLUCC 10-0711 T | Thailand |
|
|
|
|
|
BRIP 12044 T |
|
Australia |
|
|
|
|
BRIP 12521 T |
|
Australia |
|
|
|
|
BRIP 16732 T |
|
USA |
|
|
|
|
BRIP 10972 T |
|
Australia |
|
|
|
BRIP 15854 |
|
Australia |
|
|
|
|
|
BRIP 12942 T |
|
Australia |
|
|
|
BRIP 12555 |
|
Australia |
|
|
|
|
|
IMI 164633 T |
|
India |
|
|
– |
|
MFLUCC 11-0422 | unknown |
Thailand |
|
|
|
|
BRIP 12522 T |
|
Australia |
|
|
|
|
CBS 186.50 | Soil | India |
|
|
|
|
CBS 246.49 T |
|
USA |
|
|
|
|
CBS 135941 T |
|
India |
|
|
– |
|
CPC 28829 T |
|
Thailand |
|
|
|
|
UTHSC 07-2764 T |
|
USA |
|
|
– |
|
BRIP 24170 | air | Australia |
|
|
|
BRIP 24261 T |
|
Australia |
|
|
|
|
|
BRIP 61680b |
|
Australia |
|
|
|
|
CBS 192.29 T |
|
Japan |
|
|
|
|
BRIP 13066 T |
|
Australia |
|
|
|
|
BRIP 13524 T | Indonesia |
|
|
|
|
|
CBS 419.78 | Netherlands |
|
|
– | |
|
CPC 28810 T |
|
Thailand |
|
|
|
|
BRIP 12846 T |
|
Australia |
|
|
|
|
CBS 193.62T | air | Pakistan |
|
|
|
|
CBS 189.48 | Indonesia |
|
|
– | |
|
BRIP 12538 T |
|
Australia |
|
|
|
|
CBS 187.50 |
|
Indonesia |
|
|
|
|
CBS 210.79 | Romania |
|
|
– | |
|
BRIP 23186 T |
|
Australia |
|
|
|
|
BRIP 57412 T |
|
Australia |
|
|
|
|
BRIP 11987 T |
|
USA |
|
|
|
|
BRIP 14579 T |
|
India |
|
|
|
|
CBS 284.91 T |
|
Australia |
|
|
|
|
CBS 136985 T |
|
USA |
|
|
|
|
CBS 156.60 T | air | USA |
|
|
|
|
CBS 102.42 T | soil | France |
|
|
|
|
CBS 334.64 |
|
USA |
|
|
– |
|
CBS 630.82 T |
|
Solomon Islands |
|
|
– |
|
BRIP 14530 T |
|
Australia |
|
|
|
|
CBS 137.29 |
|
Japan |
|
– |
|
|
BRIP 12259 T |
|
Australia |
|
|
|
|
CBS 730.96 T |
|
USA |
|
|
|
|
CBS 131274 T |
|
USA |
|
|
|
|
BRIP 12900 T |
|
Australia |
|
|
|
BRIP 13983 |
|
Australia |
|
|
|
|
|
CBS 197.29 T |
|
Japan |
|
|
|
|
CBS 144.63 T | USA |
|
|
|
|
|
BRIP 12919 T |
|
Ghana |
|
|
|
|
IMI 129790 T |
|
India |
|
|
|
|
BRIP 11983 T | soil | Algeria |
|
|
|
|
CPC 28800 T |
|
Thailand |
|
|
|
|
CBS 160.58 |
|
USA |
|
|
|
|
CBS 169.53 T |
|
Vietnam |
|
|
|
|
CBS 470.90 T |
|
Australia |
|
|
|
|
CBS 156.35 T | air | Indonesia |
|
|
|
|
CBS 308.67 T |
|
South Africa |
|
|
|
|
BRIP 14642 T |
|
Australia |
|
|
|
|
CBS 350.90 T |
|
Australia |
|
|
|
|
CBS 190.48 T |
|
Canada |
|
|
|
|
BRIP 27703b T |
|
Australia |
|
|
|
|
BRIP 14541 T |
|
USA |
|
|
|
|
CBS 143.64 T |
|
India |
|
|
|
|
CBS 376.65 T |
|
UK |
|
|
|
|
CPC 28808 T |
|
Thailand |
|
|
|
|
UTHSC 09-2092 T |
|
USA |
|
|
– |
|
UTHSC 08-3458 |
|
USA |
|
|
– |
|
BRIP 13165 T |
|
Australia |
|
|
|
|
BRIP 4358 T | air | Australia |
|
|
|
|
BRIP 4371 T |
|
Australia |
|
|
|
|
CBS 624.68 T |
|
USA |
|
|
|
|
BRIP 12554 T |
|
Australia |
|
|
|
|
CBS 149.71 | unknown | Nigeria |
|
|
– |
|
CBS 222.96T | soil | Papua New Guinea |
|
|
|
|
BRIP 15900 T |
|
Australia |
|
|
|
|
CBS 274.52 | soil | Spain |
|
|
|
|
BRIP 23040b T |
|
Australia |
|
|
|
|
CBS 656.74 T | soil | Egypt |
|
|
|
|
CBS 173.55 |
|
USA |
|
|
– |
|
BRIP 12375 T |
|
Australia |
|
|
|
|
BRIP 14834 T |
|
India |
|
|
|
|
ATCC 44764 T |
|
Japan |
|
|
|
|
CBS 146.63 T |
|
India |
|
|
|
|
CBS 221.52 T |
|
Vietnam |
|
|
– |
|
CPC 28815 T |
|
Thailand |
|
|
|
|
CBS 537.75 |
|
New Zealand |
|
|
– |
|
CBS 150.63 |
|
India |
|
|
|
|
BRIP 14817 T |
|
Australia |
|
|
|
BRIP 17068b |
|
Australia |
|
|
|
|
BRIP 17439 |
|
Australia |
|
|
|
1ATCC: American Type Culture Collection, Manassas, Virginia, USA; BRIP: Queensland Plant Pathology Herbarium, Brisbane, Australia; CBS: Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands; CPC: cultures of Pedro Crous, housed at Westerdijk Fungal Biodiversity Institute; ICMP: International Collection of Microorganisms for Plants, Auckland, New Zealand; IMI: International Mycological Institute, CABI-Bioscience, Egham, United Kingdom; MFLUCC: Mae Fah Luang University Culture Collection, Chiang Rai, Thailand; UTHSC: Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas, USA.
TEx-type isolates.
GenBank accessions derived from this study are shown in
Isolates were grown on
Unique fixed nucleotide positions were used to characterise and describe two cryptic species (see applicable species notes). For each of the cryptic species that was described, the closest phylogenetic neighbour was selected (Fig.
Phylogenetic tree based on maximum likelihood analysis of the combined multilocus alignment. RAxML bootstrap values (bs) greater than 70% and Bayesian posterior probabilities (pp) greater than 0.7 are given at the nodes (bs/pp). Novel species names are highlighted in blue. Ex-type isolates are marked with a T. The outgroup is
Approximately 800 bp of the ITS region, 598 bp of the partial region of the
Australia, Queensland, Beaudesert, from leaf spot on
In recognition of Dr Dean R. Beasley, an Australian plant pathologist, for his dedication and numerous innovative contributions to the curation and promotion of the Queensland Plant Pathology Herbarium (BRIP).
Australia, Queensland, Atherton, from leaf spot on
Australia, Queensland, Beerburrum, from blackened inflorescence of
Named after the town Beerburrum, where the holotype was collected.
Australia, Queensland, Beerburrum, New South Wales, Yetman, blackened inflorescence of
India, Poona, from leaves of
Multilocus phylogenetic analyses placed the ex-type culture of
Australia, Queensland, Eudlo, from rotted fruit of
Named after Dr Lindel (Lindy) M. Coates, an Australian plant pathologist in recognition of her contributions to the study of post-harvest fruit pathology.
Australia, New South Wales, Alstonville, isolated from the air in a mango orchard, 11 Mar. 1991,
Australia, Queensland, Brisbane, from leaf spot on
Named after Dr Robert (Bob) Chester Colbran (1926–2010), an Australian nematologist and Director of the Plant Pathology Branch, Queensland Department of Primary Industries, in recognition of his significant contributions to Australian plant pathology.
Only one other species,
Australia, New South Wales, Yetman, from inflorescence on
Named after
Australia, Queensland, from mouldy grain of
Named after Dr Kenneth G. Pegg AM (member of the Order of Australia), in celebration of his 60 years of dedication to plant pathology in Australia and to thank him for his generous mentorship.
Australia, Queensland, Lamington National Park, from
Named after the locality, Lamington National Park, where the holotype was collected.
Australia, Victoria, Hopetoun, from
Named after the collector, Martin Mebalds, in recognition of his contributions to Australian plant pathology and biosecurity.
Australia, New South Wales, Tweed Heads, from necrotic leaf on
The multilocus phylogenetic analyses showed that
Australia, Northern Territory, Daly Waters, from leaf spot on
Named after Ron A. Peterson, an Australian plant pathologist, in recognition of his contributions to tropical plant pathology.
The multilocus phylogenetic analyses placed
Australia, Queensland, Warwick, from leaf spot on
Named after Gregory (Greg) J. Platz, in recognition of his contributions to Australian cereal plant pathology for the past 30 years, as well as his prowess as an international and Queensland rugby league footballer.
The multilocus phylogenetic analyses indicated
Australia, Queensland, Brisbane, isolated from air, 22 Jun. 1963,
Named after Dr Robert (Bob) G. Rees, an Australian plant pathologist, in recognition of his extensive contributions to cereal pathology.
The multilocus phylogenetic analyses indicated
Australia, Queensland, Musselbrook Reserve, leaf of
Named after
Based on multilocus phylogenetic analyses,
Australia, Queensland, Torres Strait, Warraber Island, from leaf spot on
Named after the locality, Warraber Island in the Torres Straits, where the specimen was collected.
Multilocus phylogenetic analyses placed
Although the ITS locus is the universal barcode marker for fungi (
Eight
It is not known whether the species described here are pathogens, endophytes or saprobes. It is also unclear as to whether these species are native or introduced.
The authors wish to thank Dr John L. Alcorn (former curator of the BRIP) for his support, as well as acknowledge his foresight in preserving the cultures examined in this study.