Corresponding authors: Bao-Kai Cui (
Academic editor: D. Begerow
Xing J-H, Sun Y-F, Han Y-L, Cui B-K, Dai Y-C (2018) Morphological and molecular identification of two new
Some
The examined specimens were deposited in the herbarium of the Institute of Microbiology, Beijing Forestry University (
The following abbreviations are used:
The CTAB rapid plant genome extraction kit-DN14 (Aidlab Biotechnologies Co. Ltd., Beijing, China) was used to extract total genomic DNA from dried specimens according to the manufacturer’s instructions with some modifications (
Species, specimens, geographic origin and GenBank accession numbers of sequences used in this study.
Species name | Voucher no. | Geographic origin | GenBank accession numbers | References | ||
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Cui 13817 (holotype) | Fujian, China | this study | |||
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Cui 14578 | Guangdong, China | – | this study | ||
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Cui 16340 | Guangxi, China | – | – | this study | |
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Dai 12588 (holotype) | Durban, South Africa |
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– |
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WD 2028 | Japan |
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WD 2085 | Japan |
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Dai 16336 (holotype) | Guangdong, China | this study | |||
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Dai 16337 | Guangdong, China | this study | |||
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Dai 16338 | Guangdong, China | this study | |||
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Dai 16339 | Guangdong, China | this study | |||
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CBS 100131 | NC, USA |
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CBS 100132 | NC, USA |
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CBS 139793 (type) | Pretoria, South Africa |
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– | – |
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CMW 43670 | Pretoria, South Africa |
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– | – |
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Dai 16431 | South Africa | this study | |||
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CBS 139792 (type) | Pretoria, South Africa |
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– | – |
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Dai 15970 | Africa |
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Dai 15971 | Africa |
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Dai 11995 | Yunnan, China |
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Yuan 6337 | Guangxi, China | this study | |||
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Cui 13982 | Guangxi, China | this study | |||
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GDGM 44489 | Xizang, China |
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– | – |
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GDGM 44490 | Xizang, China |
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– | – |
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Dai 15601 | Xizang, China |
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Wu 1006-38 (holotype) | Hubei, China |
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Cui 14342 | Sichuan, China | this study | |||
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Cui 14375 | Sichuan, China | this study | |||
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JV 1008/31 | USA |
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this study | ||
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JV 1008/32 | USA |
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this study | ||
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K 175217 | UK, Europe |
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Cui 14404 | Sichuan, China | this study | |||
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Cui 14405 | Sichuan, China | this study | |||
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LIP SW-Mart08-44 | Martinica |
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– | – |
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LIP SW-Mart08-55 (type) | Martinica |
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– | – |
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He 2240 | USA | this study | |||
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CWN 04670 | Taiwan, China |
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Dai 9447 | Hainan, China |
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– |
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Cui 14373 | Sichuan, China | this study | |||
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SPC9 | Brazil |
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– | – |
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60119011 | Brazil | – | – | this study | |
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URM 83346 | Brazil |
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– | – |
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Cui 13918 | Hainan, China | this study | |||
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Cui 13880 | Hainan, China | this study | |||
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Cui 14443 | Hainan, China | this study | |||
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Cui 14444 | Hainan, China | this study | |||
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Rivoire 4150 | France, Europe |
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– | – |
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CBS 194.76 | Netherlands, Europe |
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– |
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HKAS 58053 (type) | Cameroon, Africa |
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– | – |
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HKAS 58054 | Cameroon, Africa |
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– | – |
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HKAS 58055 | Cameroon, Africa |
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– | – |
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Dai 15785 | Shandong, China | this study | |||
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Dai 15787 | Shandong, China | this study | |||
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Dai 15791 | Shandong, China | this study | |||
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Wei 5327 | Hainan, China |
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this study | |
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Cui 13835 | Hainan, China | this study | |||
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He 1232 | Guangxi, China |
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this study | ||
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Yuan 3490 | Yunnan, China |
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– |
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Dai 16434 | Hainan, China | this study | |||
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Dai 12751b | CT, USA |
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Cui 14110 | Jilin, China | this study | |||
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Cui 14112 | Jilin, China | this study | |||
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CBS 219.36 | Philippines |
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– | – |
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Wei 5032 | Hainan, China |
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– | – |
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Dai 16809 | Thailand | this study | |||
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FL-02 | FL, USA |
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FL-03 | FL, USA |
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Outgroup | ||||||
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Cui 9011 | Guangdong, China |
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TC-02 | Vietnam |
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– |
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* Newly generated sequences for this study.
Bold names = new species.
Besides the sequences generated from this study, other reference sequences were selected from GenBank for phylogenetic analyses. Sequences were aligned in MAFFT 6 (
Phylogenetic analyses in this study followed the approach of
The combined
CHINA. Fujian Prov., Pingtan County, on living tree of
Phylogeny of the new
Basidiomata of
Microscopic structures of
Microscopic structures of
A white rot.
CHINA. Guangdong Prov., Maoming, Dianbai, on living trees of
CHINA. Guangdong Prov. Zhanjiang, Dianbai, on living tree of
a white rot.
CHINA. Guangdong Prov., Zhanjiang, Dianbai, on living trees of
The two new
Morphological differences between the two new
Species | Pileal surface | Context | Cuticle cells | Shape of basidiospores | Size of basidiospores |
---|---|---|---|---|---|
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reddish brown to dark brown | homogeneous, black melanoid band present | thin-walled, septate | almond-shaped | (8–)9–10.5(–11) × (3.5–)4–5 µm (with the turgid vesicular appendix excluded) (8–)9–11.3(–12) × (3.8–)4–5.2 µm (with the turgid vesicular appendix included) |
|
reddish brown | not fully homogeneous, black melanoid band absent | thick-walled to subsolid, non-septate | ellipsoid | (8–)8.5–9 (–10) × (4.2–)5.5–6.5(–7) µm (with the turgid vesicular appendix excluded)(8.3–)9–10.2(–11.5) × (4.5–)5–6(–7) µm (with the turgid vesicular appendix included) |
In the phylogenetic tree inferred from
In the phylogenetic tree, we obtained
In conclusion, both morphology and phylogeny inferred from the combined
The research was financed by the National Natural Science Foundation of China (Project No. 31670016) and the Fundamental Research Funds for the Central Universities (Project No. 2016ZCQ04).